Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0013012

Physical Location & Seq

Genomic Coordinates Reverse (-)

77840602 .. 77854017

Gene Structure 13416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG548700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7419 bp

ATGGGCGATGGAGGAGTCGCATGCATGCCTTTGCAGCATAATATCATGGACAGGTTTCCAATTCCGGAGAAGACTGCGCTTTGCGGAGGCAATACTAATACTAATGCTACTACTACTACTACCACCGCTAACAATGGCTTCAATTCCAAGCCGGTTGTTAAGAAGAAGGTAATGAAGATCAAGAAGAGGATTGTCAAGAAGCCCAAGGCAAAAAGCAACAGTAACGTAGAGTCGGGGAAGAGTGAGCTGGGATTGGTTAAAGGTGAGAAGAGTGATACTACTAAGGAAGCAGAGAAGAGTGGTGCCAAGGAAGCAGCCGAGAAGAGTGGTGTGAAGGAAGCCGAGAAGGGTGCTGTAAAGGAAGCTGAGAACAGTGCTGCTCAGCAAGTTGAGAACGTCGAAAATGGTGAGGATAAGAAGGAGGAAGTGGAAGAGGGTGAATTGGGGACTTTGAATGGTGAATTTGTTCCGGACAAGTGGCGGAGAAGCGAGGTTGAGAAGGAGGAGATTGGGGGTGAGAAATGGAGGAGAAGCGAGGTGGAGAAGGGAGAGTCTTTTTCTGGGAAATGGCGTAGAGGTGATGCAGAGAAGGCAGATATATATCCGGAGAAGAGTAGAAATAGTGAAGCTGAATTCGGCTCGTGGAGACCACCCAAGGATGAAATTGAAAGAGGTGAGTATATTCCAGATAGATGGCAAAAAGGAGAGCTGGCCAGAGATGACTACAGTAACCGAAAAATGCCCGCCAGGTACGATATGGGTGGGAGGGGCAGGGGAAGGGGCTGGAAATTTGAGTCCCCTTCTGGCAAGTATCCAAATGATGACGGGTTTAGAAGGAAAGAATATGGTAGAGGTGGGGGTCAGCACAGCAAGAGTACTTTCAGGTGGGATAGTGGCCAGGAGAGGAATACAAGGATCAGTTCCAAAATTGTTGATGATGAGGGTGTCTACAAGAATGAGTACAGCAGCGAGTACAGCAATGGTAAGTATCAAGCAAAGGATTACTCTTCTGTTAATCGGTTGAAGAGGTATGGTCCTGATTCAAATAGTAGTGAGCGGAAGCACTATGGAGATTATGGGGATTATGCGGGTTTAAAAAGTCGCAAGCTTTCTGATGAAAGTAATCGCTCTGCTCACTCTGAGCATTATTCACACCGTTCTGGGGAGAGATCTTATCGAAATCCTTCTTTTAGACCAGCATCAGACAAGTACTCTTCCAGGCACTATGAACCTGCTTTGTCTTCCAGAGTGGTTTATGACAGGCATGGGCGAAGTCCAGGTCTCTCTGAGCGGTCCCCACGTGACAGGTCCCGGTATTATGATCACCTGGACCGGAGTCCAGTGCGCCGTGAGAGATCTCCATATGATCTAGAGAGGTCCCCTTATGGCCGTGAGAAATCACCATATGGTCGTGAGAAATCTCCATATGTTCGTGATAAATCTCCATATCTTCGTGACAGATCTCCATATGTTCGCGACAAGTCTCCATATGCTCGCGAGAAGTCACCATATGGACGTGAAAGATCTCCATATGTTCGTGACAGATCTCCTTATGTTCTGGAGAGATCTCCATATGAACGGAGCCGTAATTATGATCACAGAAACCGCAGTTTGTCTCCACAAGATCGACCTAGGTACCATGATCGCAGGGATCACACCCCAAACTATTTGGAGCGGTCACCACGTGGTGACCGGGGTAGAGCTTCTAGTTATAGAGGTACAGGCCGCAAAAGTGGAGCAAGTGATAGGCGCAACTCCCAATATAAAGCGCACGAAGATAAGCTGGTGCAGAAGGATCCCACGGGAACGGACTCAAATTCCTCTGCCAAAGAATGTCAGGATAGAAGCAGTGTTCTTGACATTAATGGAACTGTGGAGACAAACGCCAATTCTGAGTCTCACAAAGAAGAACCGTCTCAAAGTCCAAATATAAATTGCAAAGAAACTTCTCATATTAGTGCAGCCCCTCCTGAAGAGCTGCCTTCTATGGAAGAAGATATGGATATATGCGACACACCACCACATGTCCCGGTGATTGCTGATTCATCCACAGGGAAATGGTTTTACCTTGATTATTATGGTGTGGAATGTGGGCCTTCCAAATTATGTGAACTTAAAGCACTTGTTGAAGAAGGAGCTCTGATGTCTGATCACATGGTCAAGCACTCAGACAGTGATAGGTGGGTAACTGTTGAAAATGCAGTTTCGCCATTGATTACCGTAAATTTTCCATCCATTGTATCGGACTCGATAACTGGACTAGTAAGCCCTCCAGAAGCTCCTGGCAATCTATTGGCAGATATTGGACATACTGGGCACTATGGTACTCAGAGTGGGAGTTTGCCGGGGTTGTGTGCAGATGTTGCTTCTGAGCCTCTAGAAGATTTGCACATTGAAGAAAGGGTTGGGGCTCTGATGGAAGGTTTAACAGTTATTCCTGGCAGGGAACTTGAAGCTGTTGGAGAAGTTCTGCAAATGTCATTTGAATCTGCTCAACTGGAAGTATGGGGAAACAGTGAAGGTCTCTCTCAAGGTCATATCGGTGAACAGAATGATCAGAAAACTGAGGAACCACGATTTTCTGACATTAAAATGAAAGAAGCAGCAGAAATAGGGTTGACTTTACCCTCTGACAAAGATGCTTTTGCTTGTGGTGATTCCGGTGACTGGTTTTCTGGTCGATGGTCATGCAAGGGTGGTGATTGGAAAAGGAACGAAGAAGGTGTGCAAGATGGGTCTTCTAGAAAGAAACTTGTTGTCAATGATGGTTTCCCATTATGCCAGATGTCAAAATCTGGGTATGAAGACCCTCGATGGCAAAGAAAAGATGAACTGTATTATCCTTCACAGAACAGAAGGCTTGATCTACCTACTTGGGCTTTTTCATGTCCAGATGATGCTAATAGAGTGTCTCAAAGTAAGCCAACTGTTATAAAAGGAGTAAAAGGAACTATCCTTCCGGTAGTCAAGATAAATGCATGTGTTGTAAAAGACCACGGTTCATTTGTTTCTGAGCCGCGGATAAAAGCACGAGGAATGGAAAGGCATCCTTCGAGGTCTGCTCGGTCATATTCCGCAAGTAGTGATGGCAAGAGATCATCTGGCGATGGTGATTCTCAAATGAAACCAGTAGGTGACCGAGGCTCAAAGGGATCTTCCAAATGCGTTTCCTCCATTAAAATCCCCAAAGACCGTATTGGCACAGTTGATGACTTGCAATTGCATTTGGGAGACTGGTACTACCTTGATGGTGCTGGGCATGAACGAGGACCTTCATCATTCTCTGAGCTACAGGCCTTAGTTGATCAAGGAGTGATCCTGAAACATAGCAGTGTGTTCCGGAAATTTGATAAAGTCTGGGTTCCAGTTACCTCTGCCATTGAGACTTCTGATGCTACCAAAATGAATAAGGAGAAGAACAGAACCTCAAGTAATTTTTCAGGGCAATCTCAAAGTGTTACACTTGCAGAATCAAAAACAAATTTGAGTTGGTTGCAGAATCTGCACCCTCAGTTCATTGGTTATACATGCGGAAAGCTTCATGAATTGGTGATGAAATCTTATAAAAGTCGGGAGTTTGCTGCAGCCATAAATGAGGTTTTAGATCCATGGATTAATGCAAGGCAGCCAAAAAAAGAGTTGGACAAGCACATGTACTGGAAAGCAGACGGTGATGCACGTTCTTCTAAAAGAACTCGGTTGCTGGTTGATGAAAGTGAAGAAGACTATGACGCAGGAGAGGACTTGCAAACAATAGAAAAGGATGAATCCGCATTTGAGGATCTAATTGGTAATGCTTCTTTCTTCAGAGAAGACGGCTTGAGTTATGGATCAGAGATGGCAAGCTGGGGTTTATTGGATGGACATGTGCTGGCACGGGTCTTTCATTTTTTAAGATTAGACATGAAATCTCTTACCATTGCTTCTCTGACTTGTAAGCACTGGAGAGCTGCGGTCAGTTTTTACAAGGATATTTCAAGACAGGTAGACTTGTCATCCTTGGGTCCCAACTGCACCGACTCCATGATTGTGAAGATTATGAGTGGTTATGGCAAAGAGAAGATTAATTCTATGGTTCTAATTGGTTGCACAAACATCACTCCCCACACTCTTGAAGAGATTCTTGGTTCACTTCCTTGTCTATCTACCATAGATATTAGGGGTTGCAACCAGTTTGGTGAGTTGGTCATTAAATTTCAGAACCTGAACTGGATTAAGAGCCGAAGCTCACGTGGTACAAAGATACATGATGAATCCAATTCAAAATTAAGGAGTCTGAAATATATCAGTGAGAAATCTTCTTATGTTTCGAGAAGTAAGGTTCTGGGTAATGATATGGATGATTTTAGTGAACTCAAAGTGTACTTTGATAGTGTGGATAAGAGAGACTCAGCTAATCAATCATTCCGGGGAAGTTTATACAAACGTTCAAAACTATTTGATGCTAGACGGTCCTCATCCATTCTATCTAGGGATGCTCGTATGAGGCGGTTGTCCATTAAGAAATCAGAAAATGGTTATAAGAGGATGGAGGAATTTGTTGCTTCAAGTCTGAAGGACATCATGAAGGAGAATACATCTGATTTCTTTGTACCCAAGGTTGCTGAAATTCAAGATAGAATGAGAAATGGGTATTACATTCGCCGAGGATTGAGTTCTGTGAAGGAGGATATCAGTCGAATGTGCAGGGATGCAATTAAAGCAAAGAATCGTGGTGAAGCTGGAGATATTAATCACCTGAATCACATTATTAATTTATTTATACAACTTGCCACACGTTTGGAAGGGGCTTCCAAGTCTTCTGATGCAAGGGATGAGCTGATCAAGTCGTGGGAAGATGATACATTTGCAGGGGTTTCTTCTGCCTCCAAGGGTAAAAAGAAGCTCAATAAGACATCTGAAAGGAAGTTGAGGAGTAATGGTTCCTTTATGAATGGTAGTGTGGATAATGGAGAATATGCATCTGATCGAGAAATCAGAAGGCGTTTATCCAAGTTGAATAAAAAATCTATGGATTCAGAAAGTGAAACATCTGATGATCTTGATAGGTCATCTGAAGATAGCAAGAGCAATACTGAGAGTACTGCCTCGGATACAGAAAGTGACTTGGAATCTGGGTCACAGAGTCAACCTGGGCAATCAACAGCAGATGGATGCTTAGCTCATGATGAGGGTTCAGATTCTGTGACTGATGATCGTGAGTGGGGAGCTCGCATGACCAAATCAAGCTTGGTTCCTCCTGTTACGAGAAAATATGAAGTCATTCATGAATATGTTATTGTATCGCATGAAGAGGATGTAAAGCGGAAGATGCAGGTATCTTTGCCAGATGACTATGTTGAGAAGCTTTATTCGCAGAAGAATGGAACTGAGGAGTCTGATATGGAACTTCCTGAAGTCAAGGACTACAAGCCCAGGAAAATGCTTGGAGATGAGGTTCTAGAGCAAGAGGTTTATGGAATTGATCCCTACAGCCATAATCTTTTACTTGATTCCATGCCAGAGGAGTTGGATTGGCCTCTTTTAGAAAAGCATCTGTTTATAGAAGATGTGCTTCTTCGCACCCTGAATAAGCAAGTTAGACACTTTACTGGTACCGGAACTACTCCCATGATCTATCCTTTGCGGCCCGTTGTTGAAGATATTCGGAAAACTGCGGAAGAGGATAGTGATATTCGAACTGTGAGAATGTGCCAGGGTATTTTGAAGGCCATAGACAGTCGCCCTGATGATAAATATGTTGCTTATAGAAAGGGGCTCGGCGTCGTTTGCAACAAAGAAGAAGGTTTTGGAGAAGAAGATTTTGTTGTGGAATTTTTGGGGGAGGTATATCCTGTTTGGAAATGGTATGAGAAGCAAGATGGGATTCGATCTCTGCAGAAAAACAGTAAAGATCCAGCTCCGGAATTTTACAACATTTATCTTGAAAGGCCCAAGGGTGATGCTGATGGGTATGATTTAGTTGTTGTTGATGCCATGCATAAAGCAAACTATGCAAGTAGAATTTGTCACTCGTGCCGACCTAATTGTGAAGCAAAAGTTACTGCTGTCGATGGTCGTTACCAGATCGGTATCTACACTGTACGTAAAATTCAATATGGTGAAGAGATCACATTTGACTACAACTCTGTTACGGAGAGTAAGGAAGAATATGAAGCATCAGTTTGTTTGTGTGGCAGTCAAGTTTGCCGGGGAAGTTACCTGAATTTGACAGGCGAAGGAGCATTCCTGAAGGTGCTGAAGGATTGGCATGGAATTCTGGATCGTCATCATCTAATGCTGGAAGCTTGCGAGTTAAATTCAGTATCTGAGGAGGATTATCTTGACCTGGGAAGAGCTGGTTTAGGCAATTGTTTGCTTGGTGGGTTGCCAGATTGGGTGATTGCATATTCAGCTCGTTTGGTGAGGTTCATAAACTTCGAAAGAACAAAGCTTCCTGAGGTGATTTTAAAGCACAACTTAGAAGAAAAGAGGAAGTATTTCTCAGATATATGTCTTGAGGTTGAGAAGAGTGACGCGGAGGTTCAGGCTGAGGGTGTGTTCAACCAAAGGCTTCAGAATTTGGCTGTTACTCTTGACAAGGTGAGGTATGTTATGAGCTCTGTATTTGGCAACCCAAAGAATGCTCCACCTCCACTGGAGAGGTTAAGTCCAGAAGAAGCTGTTGGCTTTGTATGGAAGGGAGAAGGATCAGTTGTTGAGGAACTCCTTCAGAGCATGGCTCCTCATGTGGAGGAACAATTGCTAAATGATCTCAAGTCCAAGATGCTTGCTCATGATCCATCGAGTTCAGATGACATTTGGAAAGAACTTAAAAGATCGTTACTATGGTTGAGAGATGAGGTCCGGAATCTTCCATGTACATACAAGTCTCGGCATGACGCTGCTGCTGACTTGATCCATATTTATGCATACACAAGGTGCTACATTAGAATACGGGAATACAAATCAGTAACTTCACCGCCGGTTTACATTAGTCCACTTGACTTGGGCCCCAACTACACTAACAAATCGGGGGCAGATTTTCAGGAGTATTGCAAGACTTACGGGGAGAATTATTGTTTAGGGCAGCTATTTTTTTGGTATAATCAGTCTAATGCAGAGCCAGATTGTAGTATTGCTAGAGCAAGTAGGGGTTGCTTATCATTACCTGATATCGGTTCCTTCTATGCCAAGATTTCGAAACCTACACGGCAGCGTGTTTATGGTCCAAAGGCTGTCAAATTTATGCTTTCAAGGATGGAGAAGCAGCCCCAAAAACCTTGGCCCAAGGATCGAATATGGTCATTTAATAGCTCCCCAAAAGTTCTTGGCAGTCCAATGCTAGATGCTGTTGTGAATAACTCTTTCTTGGACAAAGAAATGTTGCATTGGTTAAAGCACAGACCTGCAATATATCAAGCTATGTGGGATCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0006325 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008757 GO:0009791 GO:0009908 GO:0009987 GO:0010228 GO:0016043 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018024 GO:0018193 GO:0018205 GO:0019538 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034968 GO:0036211 GO:0042054 GO:0042800 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0051276 GO:0051568 GO:0061458 GO:0071704 GO:0071840 GO:0090567 GO:0099402 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SET

Protein Analysis

2472

Amino Acids

279.82

Weight (kDa)

6.6

Isoelectric Point (pI)

53.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GYF_ATXR3	PF25531	685 - 732	2.1e-29	Histone-lysine N-methyltransferase ATXR3, GYF domain
GYF_ATXR3	PF25531	1077 - 1108	7.9e-08	Histone-lysine N-methyltransferase ATXR3, GYF domain
SET	PF00856	1834 - 2042	1.2e-12	SET domain
SDG2_C	PF19633	2053 - 2472	8.9e-237	Protein SET DOMAIN GROUP 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15180
fragaria_vesca	FvH4_6g43760
malus_domestica	MD09G1103200.v1.1 MD17G1091000.v1.1
prunus_persica	Prupe.3G222600_v2.0.a1
pyrus_communis	pycom09g02660 pycom17g08780
rosa_chinensis	RchiOBHm_Chr2g0160501
rosa_laevigata	RLG00000021218
rosa_multiflora	Rmu_sc0000674.1_g000001
rosa_roxburghii	Rroxscaffold_2G00089780
rosa_rugosa	Rorug02G0483400 Rorug02G0483400
rosa_samantha	Rh2AG548700 Rh2BG562400 Rh2CG533000 Rh2DG571100
rosa_wichuraiana	Rw2G045430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2933, 3564, 4521
Acc36I	ACCTGC	3 cut(s)	1240, 5305, 7396
Acc65I	GGTACC	2 cut(s)	1635, 5594
AccB1I	GGYRCC	3 cut(s)	302, 1635, 5594
AccBSI	CCGCTC	3 cut(s)	1057, 1291, 1675
AccI	GTMKAC	2 cut(s)	948, 3987
AccII	CGCG	4 cut(s)	1476, 1497, 3019, 6518
AccIII	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
AclI	AACGTT	1 cut(s)	4426
AcoI	YGGCCR	3 cut(s)	711, 895, 1387
AcuI	CTGAAG	9 cut(s)	1992, 3790, 4574, 5076, 5415, 6251, 6260, 6539, 6695
AcvI	CACGTG	3 cut(s)	1301, 1685, 4232
AcyI	GRCGYC	1 cut(s)	5763
AdeI	CACNNNGTG	3 cut(s)	1685, 1688, 6921
AfiI	CCNNNNNNNGG	3 cut(s)	1162, 2667, 7157
AflIII	ACRYGT	4 cut(s)	2023, 3651, 3865, 4775
AhlI	ACTAGT	1 cut(s)	2260
AjiI	CACGTC	1 cut(s)	1517
AjuI	GAANNNNNNNTTGG	8 cut(s)	2388, 2420, 4107, 4139, 5772, 5804, 6648, 6680
AloI	GAACNNNNNNTCC	8 cut(s)	2941, 2973, 4906, 4938, 5421, 5453, 6418, 6450
Alw21I	GWGCWC	3 cut(s)	2140, 5212, 6602
AlwNI	CAGNNNCTG	4 cut(s)	3502, 4204, 5183, 6308
Aor13HI	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
ApaI	GGGCCC	1 cut(s)	6995
ArsI	GACNNNNNNTTYG	2 cut(s)	1264, 1296
AseI	ATTAAT	5 cut(s)	1863, 3615, 4065, 4731, 4752
Asp700I	GAANNNNTTC	7 cut(s)	465, 1183, 4119, 5262, 5643, 6479, 6708
Asp718I	GGTACC	2 cut(s)	1635, 5594
AspA2I	CCTAGG	1 cut(s)	1631
AspLEI	GCGC	4 cut(s)	79, 1347, 1752, 1771
AsuC2I	CCSGG	6 cut(s)	1312, 1694, 2030, 2346, 4409, 6191
AsuII	TTCGAA	3 cut(s)	5677, 6420, 7181
AvrII	CCTAGG	1 cut(s)	1631
AxyI	CCTNAGG	1 cut(s)	6438
BaeGI	GKGCMC	2 cut(s)	2319, 6995
BaeI	ACNNNNGTAYC	2 cut(s)	1619, 1652
BalI	TGGCCA	2 cut(s)	713, 897
BamHI	GGATCC	1 cut(s)	1795
BanI	GGYRCC	3 cut(s)	302, 1635, 5594
BanII	GRGCYC	6 cut(s)	2140, 2413, 5212, 5760, 6602, 6995
BauI	CACGAG	3 cut(s)	640, 3030, 6013
BbrPI	CACGTG	3 cut(s)	1301, 1685, 4232
BbsI	GAAGAC	7 cut(s)	77, 1233, 2730, 2811, 3729, 3819, 4791
Bbv12I	GWGCWC	3 cut(s)	2140, 5212, 6602
BbvCI	CCTCAGC	1 cut(s)	6531
BceAI	ACGGC	5 cut(s)	1332, 1374, 1569, 3832, 7208
BcgI	CGANNNNNNTGC	4 cut(s)	332, 366, 2670, 2704
BciVI	GTATCC	2 cut(s)	822, 5086
BclI	TGATCA	6 cut(s)	1321, 1594, 2149, 2554, 3304, 4821
BcnI	CCSGG	6 cut(s)	1312, 1694, 2030, 2346, 4409, 6191
BcuI	ACTAGT	1 cut(s)	2260
BfmI	CTRYAG	5 cut(s)	724, 3290, 3582, 5470, 5876
BfuAI	ACCTGC	3 cut(s)	1240, 5305, 7396
BfuI	GTATCC	2 cut(s)	822, 5086
BglII	AGATCT	6 cut(s)	1169, 1355, 1460, 1523, 1544, 1565
BlnI	CCTAGG	1 cut(s)	1631
BlpI	GCTNAGC	2 cut(s)	381, 5158
BmcAI	AGTACT	3 cut(s)	877, 1211, 5083
BmgBI	CACGTC	1 cut(s)	1517
BmrI	ACTGGG	1 cut(s)	2322
BmuI	ACTGGG	1 cut(s)	2322
BoxI	GACNNNNGTC	1 cut(s)	1335
BpiI	GAAGAC	7 cut(s)	77, 1233, 2730, 2811, 3729, 3819, 4791
BplI	GAGNNNNNCTC	4 cut(s)	3046, 3078, 6706, 6738
BpmI	CTGGAG	5 cut(s)	1580, 2256, 3964, 4743, 6659
Bpu10I	CCTNAGC	1 cut(s)	6531
Bpu1102I	GCTNAGC	2 cut(s)	381, 5158
Bpu14I	TTCGAA	3 cut(s)	5677, 6420, 7181
BpuEI	CTTGAG	5 cut(s)	2514, 3412, 3841, 6518, 6740
BpuMI	CCSGG	6 cut(s)	1312, 1694, 2030, 2346, 4409, 6191
BsaAI	YACGTR	4 cut(s)	1301, 1685, 4232, 6086
BsaBI	GATNNNNATC	4 cut(s)	600, 4731, 6071, 6267
BsaHI	GRCGYC	1 cut(s)	5763
BsaI	GGTCTC	3 cut(s)	640, 1286, 2528
BsaXI	ACNNNNNCTCC	4 cut(s)	4906, 4936, 5421, 5451
Bsc4I	CCNNNNNNNGG	3 cut(s)	1162, 2667, 7157
Bse118I	RCCGGY	2 cut(s)	151, 6964
Bse21I	CCTNAGG	1 cut(s)	6438
Bse3DI	GCAATG	2 cut(s)	985, 3918
Bse8I	GATNNNNATC	4 cut(s)	600, 4731, 6071, 6267
BseAI	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
BseJI	GATNNNNATC	4 cut(s)	600, 4731, 6071, 6267
BseLI	CCNNNNNNNGG	3 cut(s)	1162, 2667, 7157
BseMI	GCAATG	2 cut(s)	985, 3918
BseRI	GAGGAG	8 cut(s)	27, 518, 541, 4927, 5387, 5519, 6326, 6714
BseSI	GKGCMC	2 cut(s)	2319, 6995
BseYI	CCCAGC	3 cut(s)	247, 3254, 3846
BsgI	GTGCAG	6 cut(s)	1808, 1980, 2376, 3488, 3997, 4705
Bsh1236I	CGCG	4 cut(s)	1476, 1497, 3019, 6518
BshNI	GGYRCC	3 cut(s)	302, 1635, 5594
BsiHKAI	GWGCWC	3 cut(s)	2140, 5212, 6602
BslFI	GGGAC	7 cut(s)	460, 781, 1279, 1294, 1363, 2012, 3990
BslI	CCNNNNNNNGG	3 cut(s)	1162, 2667, 7157
BsmBI	CGTCTC	1 cut(s)	1920
BsmFI	GGGAC	7 cut(s)	460, 781, 1279, 1294, 1363, 2012, 3990
BsmI	GAATGC	2 cut(s)	6224, 6628
Bso31I	GGTCTC	3 cut(s)	640, 1286, 2528
Bsp119I	TTCGAA	3 cut(s)	5677, 6420, 7181
Bsp120I	GGGCCC	1 cut(s)	6991
Bsp1286I	GDGCHC	7 cut(s)	2140, 2319, 2413, 5212, 5760, 6602, 6995
Bsp13I	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
Bsp1407I	TGTACA	1 cut(s)	6860
Bsp1720I	GCTNAGC	2 cut(s)	381, 5158
Bsp19I	CCATGG	1 cut(s)	3608
Bsp68I	TCGCGA	2 cut(s)	1476, 1497
BspEI	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
BspFNI	CGCG	4 cut(s)	1476, 1497, 3019, 6518
BspHI	TCATGA	5 cut(s)	3541, 4563, 5164, 5266, 6775
BspMAI	CTGCAG	2 cut(s)	3586, 5880
BspMI	ACCTGC	3 cut(s)	1240, 5305, 7396
BspQI	GCTCTTC	2 cut(s)	1968, 6328
BspT104I	TTCGAA	3 cut(s)	5677, 6420, 7181
BspT107I	GGYRCC	3 cut(s)	302, 1635, 5594
BspTNI	GGTCTC	3 cut(s)	640, 1286, 2528
BsrBI	CCGCTC	3 cut(s)	1057, 1291, 1675
BsrDI	GCAATG	2 cut(s)	985, 3918
BsrFI	RCCGGY	2 cut(s)	151, 6964
BsrGI	TGTACA	1 cut(s)	6860
BssAI	RCCGGY	2 cut(s)	151, 6964
BssNI	GRCGYC	1 cut(s)	5763
BssSI	CACGAG	3 cut(s)	640, 3030, 6013
Bst2BI	CACGAG	3 cut(s)	640, 3030, 6013
BstACI	GRCGYC	1 cut(s)	5763
BstAPI	GCANNNNNTGC	3 cut(s)	2363, 3502, 5993
BstAUI	TGTACA	1 cut(s)	6860
BstBAI	YACGTR	4 cut(s)	1301, 1685, 4232, 6086
BstBI	TTCGAA	3 cut(s)	5677, 6420, 7181
BstDSI	CCRYGG	4 cut(s)	1800, 2995, 3017, 3608
BstEII	GGTNACC	3 cut(s)	1677, 1688, 3134
BstFNI	CGCG	4 cut(s)	1476, 1497, 3019, 6518
BstHHI	GCGC	4 cut(s)	79, 1347, 1752, 1771
BstNSI	RCATGY	7 cut(s)	24, 28, 2027, 2982, 3531, 3655, 3869
BstPAI	GACNNNNGTC	1 cut(s)	1335
BstPI	GGTNACC	3 cut(s)	1677, 1688, 3134
BstSFI	CTRYAG	5 cut(s)	724, 3290, 3582, 5470, 5876
BstSLI	GKGCMC	2 cut(s)	2319, 6995
BstSNI	TACGTA	1 cut(s)	6086
BstUI	CGCG	4 cut(s)	1476, 1497, 3019, 6518
BstV2I	GAAGAC	7 cut(s)	77, 1233, 2730, 2811, 3729, 3819, 4791
BstXI	CCANNNNNNTGG	2 cut(s)	693, 4780
Bsu36I	CCTNAGG	1 cut(s)	6438
BsuI	GTATCC	2 cut(s)	822, 5086
BtgI	CCRYGG	4 cut(s)	1800, 2995, 3017, 3608
BtgZI	GCGATG	2 cut(s)	21, 3120
BtrI	CACGTC	1 cut(s)	1517
BtsI	GCAGTG	2 cut(s)	1855, 3337
BtuMI	TCGCGA	2 cut(s)	1476, 1497
BveI	ACCTGC	3 cut(s)	1240, 5305, 7396
CaiI	CAGNNNCTG	4 cut(s)	3502, 4204, 5183, 6308
CciI	TCATGA	5 cut(s)	3541, 4563, 5164, 5266, 6775
CfoI	GCGC	4 cut(s)	79, 1347, 1752, 1771
Cfr10I	RCCGGY	2 cut(s)	151, 6964
Cfr42I	CCGCGG	1 cut(s)	3020
CseI	GACGC	4 cut(s)	3740, 5752, 6524, 6890
CspCI	CAANNNNNGTGG	3 cut(s)	6969, 7004, 7388
DraI	TTTAAA	2 cut(s)	1095, 6450
DraIII	CACNNNGTG	3 cut(s)	1685, 1688, 6921
EaeI	YGGCCR	3 cut(s)	711, 895, 1387
EciI	GGCGGA	1 cut(s)	496
Ecl136II	GAGCTC	3 cut(s)	2138, 5210, 6600
Eco105I	TACGTA	1 cut(s)	6086
Eco147I	AGGCCT	1 cut(s)	3296
Eco24I	GRGCYC	6 cut(s)	2140, 2413, 5212, 5760, 6602, 6995
Eco31I	GGTCTC	3 cut(s)	640, 1286, 2528
Eco32I	GATATC	2 cut(s)	4672, 7156
Eco53kI	GAGCTC	3 cut(s)	2138, 5210, 6600
Eco57I	CTGAAG	9 cut(s)	1992, 3790, 4574, 5076, 5415, 6251, 6260, 6539, 6695
Eco72I	CACGTG	3 cut(s)	1301, 1685, 4232
Eco81I	CCTNAGG	1 cut(s)	6438
Eco91I	GGTNACC	3 cut(s)	1677, 1688, 3134
EcoICRI	GAGCTC	3 cut(s)	2138, 5210, 6600
EcoO109I	RGGNCCY	5 cut(s)	1308, 1377, 3269, 4004, 6992
EcoO65I	GGTNACC	3 cut(s)	1677, 1688, 3134
EcoRI	GAATTC	2 cut(s)	632, 6255
EcoRV	GATATC	2 cut(s)	4672, 7156
EcoT22I	ATGCAT	5 cut(s)	26, 2980, 4963, 5982, 6913
EcoT38I	GRGCYC	6 cut(s)	2140, 2413, 5212, 5760, 6602, 6995
Esp3I	CGTCTC	1 cut(s)	1920
FalI	AAGNNNNNCTT	8 cut(s)	3034, 3066, 4107, 4139, 5538, 5570, 6443, 6475
FaqI	GGGAC	7 cut(s)	460, 781, 1279, 1294, 1363, 2012, 3990
FauI	CCCGC	2 cut(s)	751, 1081
FauNDI	CATATG	8 cut(s)	1363, 1405, 1426, 1468, 1489, 1510, 1531, 1573
FbaI	TGATCA	6 cut(s)	1321, 1594, 2149, 2554, 3304, 4821
FblI	GTMKAC	2 cut(s)	948, 3987
FriOI	GRGCYC	6 cut(s)	2140, 2413, 5212, 5760, 6602, 6995
GlaI	GCGC	4 cut(s)	78, 1346, 1751, 1770
GsaI	CCCAGC	3 cut(s)	251, 3258, 3850
GsuI	CTGGAG	5 cut(s)	1580, 2256, 3964, 4743, 6659
HgaI	GACGC	4 cut(s)	3740, 5752, 6524, 6890
HhaI	GCGC	4 cut(s)	79, 1347, 1752, 1771
Hin1I	GRCGYC	1 cut(s)	5763
Hin6I	GCGC	4 cut(s)	77, 1345, 1750, 1769
HinP1I	GCGC	4 cut(s)	77, 1345, 1750, 1769
HincII	GTYRAC	2 cut(s)	2619, 5129
HindII	GTYRAC	2 cut(s)	2619, 5129
HindIII	AAGCTT	6 cut(s)	1106, 3536, 5227, 5345, 6285, 6431
Hpy99I	CGWCG	2 cut(s)	401, 5768
Hsp92I	GRCGYC	1 cut(s)	5763
HspAI	GCGC	4 cut(s)	77, 1345, 1750, 1769
KflI	GGGWCCC	1 cut(s)	4004
Kpn2I	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
KpnI	GGTACC	2 cut(s)	1639, 5598
Ksp22I	TGATCA	6 cut(s)	1321, 1594, 2149, 2554, 3304, 4821
KspI	CCGCGG	1 cut(s)	3020
LguI	GCTCTTC	2 cut(s)	1968, 6328
MbiI	CCGCTC	3 cut(s)	1057, 1291, 1675
MfeI	CAATTG	3 cut(s)	3218, 6349, 6740
MhlI	GDGCHC	7 cut(s)	2140, 2319, 2413, 5212, 5760, 6602, 6995
MlsI	TGGCCA	2 cut(s)	713, 897
MluNI	TGGCCA	2 cut(s)	713, 897
MmeI	TCCRAC	3 cut(s)	2440, 3621, 5490
Mox20I	TGGCCA	2 cut(s)	713, 897
Mph1103I	ATGCAT	5 cut(s)	26, 2980, 4963, 5982, 6913
MroI	TCCGGA	6 cut(s)	64, 469, 604, 3339, 5902, 6846
MroXI	GAANNNNTTC	7 cut(s)	465, 1183, 4119, 5262, 5643, 6479, 6708
MscI	TGGCCA	2 cut(s)	713, 897
MslI	CAYNNNNRTG	7 cut(s)	1956, 2541, 2591, 3330, 4028, 5271, 6906
Msp20I	TGGCCA	2 cut(s)	713, 897
MspA1I	CMGCKG	1 cut(s)	3019
MunI	CAATTG	3 cut(s)	3218, 6349, 6740
Mva1269I	GAATGC	2 cut(s)	6224, 6628
MvnI	CGCG	4 cut(s)	1476, 1497, 3019, 6518
NciI	CCSGG	6 cut(s)	1312, 1694, 2030, 2346, 4409, 6191
NcoI	CCATGG	1 cut(s)	3608
NdeI	CATATG	8 cut(s)	1363, 1405, 1426, 1468, 1489, 1510, 1531, 1573
NmeAIII	GCCGAG	5 cut(s)	343, 367, 4670, 5739, 6853
NruI	TCGCGA	2 cut(s)	1476, 1497
NsiI	ATGCAT	5 cut(s)	26, 2980, 4963, 5982, 6913
NspI	RCATGY	7 cut(s)	24, 28, 2027, 2982, 3531, 3655, 3869
NspV	TTCGAA	3 cut(s)	5677, 6420, 7181
PaeI	GCATGC	2 cut(s)	24, 28
PagI	TCATGA	5 cut(s)	3541, 4563, 5164, 5266, 6775
PceI	AGGCCT	1 cut(s)	3296
PciI	ACATGT	3 cut(s)	2023, 3651, 3865
PciSI	GCTCTTC	2 cut(s)	1968, 6328
PctI	GAATGC	2 cut(s)	6224, 6628
PdmI	GAANNNNTTC	7 cut(s)	465, 1183, 4119, 5262, 5643, 6479, 6708
PmaCI	CACGTG	3 cut(s)	1301, 1685, 4232
PmlI	CACGTG	3 cut(s)	1301, 1685, 4232
Ppu21I	YACGTR	4 cut(s)	1301, 1685, 4232, 6086
PpuMI	RGGWCCY	4 cut(s)	1308, 1377, 3269, 4004
PscI	ACATGT	3 cut(s)	2023, 3651, 3865
PshAI	GACNNNNGTC	1 cut(s)	1335
PshBI	ATTAAT	5 cut(s)	1863, 3615, 4065, 4731, 4752
PsiI	TTATAA	3 cut(s)	2933, 3564, 4521
Psp124BI	GAGCTC	3 cut(s)	2140, 5212, 6602
Psp1406I	AACGTT	1 cut(s)	4426
Psp5II	RGGWCCY	4 cut(s)	1308, 1377, 3269, 4004
PspCI	CACGTG	3 cut(s)	1301, 1685, 4232
PspEI	GGTNACC	3 cut(s)	1677, 1688, 3134
PspFI	CCCAGC	3 cut(s)	247, 3254, 3846
PspOMI	GGGCCC	1 cut(s)	6991
PspPPI	RGGWCCY	4 cut(s)	1308, 1377, 3269, 4004
PstI	CTGCAG	2 cut(s)	3586, 5880
PstNI	CAGNNNCTG	4 cut(s)	3502, 4204, 5183, 6308
RruI	TCGCGA	2 cut(s)	1476, 1497
RseI	CAYNNNNRTG	7 cut(s)	1956, 2541, 2591, 3330, 4028, 5271, 6906
SacI	GAGCTC	3 cut(s)	2140, 5212, 6602
SacII	CCGCGG	1 cut(s)	3020
SapI	GCTCTTC	2 cut(s)	1968, 6328
ScaI	AGTACT	3 cut(s)	877, 1211, 5083
SduI	GDGCHC	7 cut(s)	2140, 2319, 2413, 5212, 5760, 6602, 6995
SfcI	CTRYAG	5 cut(s)	724, 3290, 3582, 5470, 5876
Sfr303I	CCGCGG	1 cut(s)	3020
SfuI	TTCGAA	3 cut(s)	5677, 6420, 7181
SgrBI	CCGCGG	1 cut(s)	3020
SmiMI	CAYNNNNRTG	7 cut(s)	1956, 2541, 2591, 3330, 4028, 5271, 6906
SmlI	CTYRAG	5 cut(s)	2529, 3427, 3820, 6497, 6755
SmoI	CTYRAG	5 cut(s)	2529, 3427, 3820, 6497, 6755
SnaBI	TACGTA	1 cut(s)	6086
SpeI	ACTAGT	1 cut(s)	2260
SphI	GCATGC	2 cut(s)	24, 28
SseBI	AGGCCT	1 cut(s)	3296
SstI	GAGCTC	3 cut(s)	2140, 5212, 6602
StuI	AGGCCT	1 cut(s)	3296
TaqII	GACCGA	2 cut(s)	3054, 3153
TatI	WGTACW	8 cut(s)	875, 960, 972, 1209, 3654, 4362, 5081, 6860
TauI	GCSGC	3 cut(s)	1728, 3019, 5629
TspGWI	ACGGA	3 cut(s)	1594, 1823, 6149
VspI	ATTAAT	5 cut(s)	1863, 3615, 4065, 4731, 4752
XbaI	TCTAGA	4 cut(s)	1369, 2377, 2741, 5440
XceI	RCATGY	7 cut(s)	24, 28, 2027, 2982, 3531, 3655, 3869
XcmI	CCANNNNNNNNNTGG	2 cut(s)	754, 5227
XmaJI	CCTAGG	1 cut(s)	1631
XmiI	GTMKAC	2 cut(s)	948, 3987
XmnI	GAANNNNTTC	7 cut(s)	465, 1183, 4119, 5262, 5643, 6479, 6708
ZrmI	AGTACT	3 cut(s)	877, 1211, 5083
Zsp2I	ATGCAT	5 cut(s)	26, 2980, 4963, 5982, 6913

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.