Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009004

Physical Location & Seq

Genomic Coordinates Reverse (-)

8193005 .. 8199754

Gene Structure 6750 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1096700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2979 bp

ATGTATATAAACACAGCCTCCTCCGCTGCCTCCTCCCTCCTCAGAGCTTCTAGGGCTCGATTTTCCTCCTCCTCCGCTGTTTCCAGAACCTTCGTCTCTTCGCCCCTCAGGCCTTCGCACCACCACTTCGCCTCCCACCGCCTCCTCAGCTCCTCCTCCGCCGCTCGCTCCTTCCCTCGCTGGAGCCACGGCGGTGTCCATTGGCGCTCCTCCTACACCCTCCGCTCCCAGATCAGAGCCGTCGCTCCCGTCATCGAACAGTTTCAGCGCAAGATCGCTTCCATGGCTTCCGAAAATCCTTTCAAGCCAAATTTGACAAGTCTTCCCAAGCCCGGAGGTGGCGAGTTTGGAAAATTCTACAGCCTTCCTTCTCTGAACGACCCGAGGATTGATAGGTTACCGTATTCTATCAGAATCCTTCTGGAATCTGCTATTCGTAATTGCGATAACTTCCAAGTCAAGAAGGAGGATGTTGAGAAAATTCTTGATTGGGAAAAGACTGCCCCAAAGCAAGTCGAAATTCCTTTCAAGCCTGCTCGTGTGCTCTTGCAGGACTTCACGGGAGTTCCAGCTGTGGTCGACCTTGCTTGTATGCGTGATGCTATGAACAAACTTGGGAGTGATTCTAAGAAGATCAATCCTTTGGTTCCTGTAGACCTTGTTATTGATCATTCAGTTCAAGTTGACGTTGCAAGATCAGCAAATGCAGTGCAGGCCAATATGGATCTTGAATTCCAGAGAAATAAGGAGAGATTCGCTTTTCTTAAATGGGGTTCTACTGCGTTCCATAATATGCTTGTTGTTCCACCTGGATCTGGCATTGTTCATCAGGTTAATCTTGAATATCTTGGACGGGTTGTTTTCAACAATGATGGCTTACTCTACCCTGATAGTGTGGTTGGAACTGATTCCCATACAACCATGATTGACGGGTTAGGAGTTGCTGGATGGGGGGTTGGAGGTATTGAAGCCGAAGCTACTATGCTTGGCCAGCCAATGAGCATGGTGTTGCCTGGTGTTGTCGGGCTCAAGTTATCTGGAAGGTTAAACAATGGTGTTACTGCTACTGACTTGGTTTTGACTGTCACACAAATACTGAGGAAGCATGGAGTTGTTGGAAAATTTGTTGAATTCTATGGCGATGGTATGGGTGAACTATCATTAGCTGACAGGGCAACTATTGCCAATATGTCCCCTGAGTATGGTGCAACCATGGGCTTCTTCCCTGTTGATCATGTTACTTTACAGTATCTCAAATTGACTGGTAGAAGTGATGAGACTGTGTCAATGATTGAAAGTTATCTGCGGGCAAATAAATTGTTTGTTGACTATAATGAGCCTCAAACAGAACGAGCATACTCATCTTACCTAGAATTGAACCTTTCAGAAGTCGAGCCCTGTATCTCAGGACCTAAGAGACCTCATGACCGTGTGACTTTGAAAGACATGAAATCTGATTGGCATGCTTGTCTTGATAACAAGGTTGGGTTCAAGGGATTTGCTATACCAAAAGAGGTGCAAAACAAAGTGGCAAAGTTTCAATTCGATGGACAGCCAGCAGAGCTTAAGCATGGTAGTGTTGTGATTGCTGCAATCACAAGTTGCACCAATACATCAAACCCAAGTGTCATGCTTGGAGCTGCTCTCGTTGCAAAGAAGGCTAGTGAACTTGGTCTACAGGTCAAGCCATGGGTTAAAACAAGTCTTGCCCCGGGTTCTGGAGTTGTTACAAAATATTTGCTACAGAGCGGATTGCAAAAGTATTTTGATCAACAAGGTTTCAATATTGTTGGATATGGGTGCACAACATGCATTGGGAATTCAGGGGATCTGCACGAAAGTGTTGCTTCTGCTATATCGGAAAATGACATTGTAGCAGCTGCTGTGCTCTCTGGAAACAGGAATTTTGAGGGCCGTGTTCATCCATTGACAAGAGCTAATTACCTTGCCTCTCCTCCTTTAGTGGTTGCTTATGCCCTTGCTGGAACGGTTGACATTGACTTTGATACGGAGCCAATTGGAACAGGGAAGGATGGTAAGAGCGTCTATTTCAGGGATATCTGGCCGTCCACAGAGGAGATTGCTGAGGTAGTGCAATCCAGCGTGTTGCCAGATATGTTCAAGAGCACTTACGAGTCAATTACTAAGGGCAACCCCATTTGGAATCAGCTATCAGTTCCCGAATCCAAACTGTACTCATGGGACCCTAATTCAACCTACATTCATGAGCCCCCATACTTCAAGGATATGACCATGTATCCTCCTGGTTCTCATGGAGTGAAGGATGCGTACTGCTTGTTGAATTTTGGTGACAGTATTACAACTGATCACATCTCCCCAGCGGGAAGCATCAACAAGGACAGTCCTGCAGCCAAGTACCTTCTTGAGCGTGGGGTGGACCGCAAAGACTTCAATTCTTATGGAAGTCGTCGTGGCAATGATGAAGTTATGGCCAGGGGAACTTTTGCCAATATCCGCCTCGTTAACAAGCTTTTGAATGGGGAAGTAGGGCCTAAAACAGTTCACATTCCTACCGGGGAGAAACTCTTTGTCTTTGATGCAGCAACTAGATACAAGGCTGATGGGCACGACACTATTGTAATAGCCGGAGCCGAGTATGGAAGTGGAAGCTCCAGGGATTGGGCCGCCAAGGGCCCAATGCTATTGGGCGTTAAAGCAGTGATTGCTAAAAGTTTCGAGAGAATCCATCGCAGTAATTTGGTGGGGATGGGTATCATTCCTCTTTGTTTCAAGGCCGGTGAGGATGCAGACACACTAGGATTGACTGGTCACGAACGGTATACCATTGACCTCCCAAGCAACATTAGTGAGATTAAACCTGGCCAAGATGTGACCGTCACAACGGACAACGGAAAAGCTTTCACCTGCACTGTTCGCTTTGACACTGAGGTGGAACTGGAGTACTTCAACCACGGAGGCATTCTTCAATACGTTATCAGGAACCTAAGCAAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031090 GO:0031667 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048856 GO:0050896 GO:0071704 GO:0071944 GO:0072350 GO:0090351 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

993

Amino Acids

108.47

Weight (kDa)

7.12

Isoelectric Point (pI)

38.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	159 - 662	4.6e-168	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	791 - 919	1e-40	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05710
fragaria_vesca	FvH4_6g43190
malus_domestica	MD09G1108800.v1.1 MD17G1096700.v1.1
prunus_persica	Prupe.3G217300_v2.0.a1 Prupe.3G217300_v2.0.a1
pyrus_communis	pycom09g03120 pycom17g09260
rosa_chinensis	RchiOBHm_Chr2g0159291
rosa_laevigata	RLG00000021152
rosa_multiflora	Rmu_sc0004800.1_g000001 Rmu_sc0007806.1_g000010 Rmu_sc0010590.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090620
rosa_rugosa	Rorug02G0475700
rosa_samantha	Rh2AG542100 Rh2BG554700 Rh2CG524800 Rh2DG564400 Rh5AG522200
rosa_wichuraiana	Rw2G044840 Rw5G048460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2894
Acc36I	ACCTGC	1 cut(s)	2894
AccB7I	CCANNNNNTGG	1 cut(s)	2640
AccBSI	CCGCTC	3 cut(s)	164, 225, 1749
AccI	GTMKAC	4 cut(s)	579, 654, 1675, 2801
AclWI	GGATC	3 cut(s)	732, 820, 1836
AcoI	YGGCCR	4 cut(s)	988, 2063, 2451, 2842
AfaI	GTAC	4 cut(s)	2195, 2291, 2378, 2924
AfiI	CCNNNNNNNGG	6 cut(s)	332, 338, 815, 1202, 1718, 2640
AflII	CTTAAG	1 cut(s)	1565
AjnI	CCWGG	6 cut(s)	808, 1012, 2263, 2453, 2633, 2839
AleI	CACNNNNGTG	3 cut(s)	192, 1839, 2909
Alw21I	GWGCWC	4 cut(s)	546, 1805, 1890, 2129
Alw26I	GTCTC	3 cut(s)	100, 1271, 1411
Alw44I	GTGCAC	1 cut(s)	1801
AlwI	GGATC	3 cut(s)	732, 820, 1836
AlwNI	CAGNNNCTG	1 cut(s)	1883
Ama87I	CYCGRG	2 cut(s)	382, 1711
ApaI	GGGCCC	1 cut(s)	2657
ApaLI	GTGCAC	1 cut(s)	1801
ApeKI	GCWGC	7 cut(s)	26, 1589, 1640, 1877, 1880, 2369, 2561
ArsI	GACNNNNNNTTYG	2 cut(s)	1985, 2017
Asp700I	GAANNNNTTC	2 cut(s)	261, 907
AspLEI	GCGC	2 cut(s)	207, 270
AspS9I	GGNCC	8 cut(s)	1409, 1912, 2203, 2398, 2510, 2643, 2653, 2654
AsuC2I	CCSGG	4 cut(s)	333, 1712, 1713, 2536
AsuHPI	GGTGA	4 cut(s)	1163, 2321, 2771, 2875
AvaI	CYCGRG	2 cut(s)	382, 1711
AvaII	GGWCC	3 cut(s)	1409, 2203, 2398
AxyI	CCTNAGG	1 cut(s)	107
BaeGI	GKGCMC	3 cut(s)	1805, 2589, 2657
BalI	TGGCCA	3 cut(s)	990, 2453, 2844
BanII	GRGCYC	5 cut(s)	58, 1029, 1398, 2232, 2657
BarI	GAAGNNNNNNTAC	2 cut(s)	2273, 2305
BauI	CACGAG	1 cut(s)	537
BbsI	GAAGAC	1 cut(s)	314
Bbv12I	GWGCWC	4 cut(s)	546, 1805, 1890, 2129
BbvCI	CCTCAGC	2 cut(s)	146, 2085
BbvI	GCAGC	7 cut(s)	13, 1576, 1627, 1867, 1889, 2381, 2573
BccI	CCATC	8 cut(s)	866, 942, 1136, 1541, 2027, 2576, 2715, 2722
BceAI	ACGGC	4 cut(s)	205, 224, 1899, 2050
BcgI	CGANNNNNNTGC	2 cut(s)	1826, 1860
BciT130I	CCWGG	6 cut(s)	810, 1014, 2265, 2455, 2635, 2841
BciVI	GTATCC	1 cut(s)	2268
BclI	TGATCA	4 cut(s)	667, 1231, 1768, 2326
BcnI	CCSGG	4 cut(s)	333, 1712, 1713, 2536
BcoDI	GTCTC	3 cut(s)	100, 1271, 1411
BfaI	CTAG	5 cut(s)	51, 1370, 1662, 2568, 2777
BfmI	CTRYAG	5 cut(s)	358, 651, 1676, 1742, 2367
BfoI	RGCGCY	1 cut(s)	208
BfrI	CTTAAG	1 cut(s)	1565
BfuAI	ACCTGC	1 cut(s)	2894
BfuI	GTATCC	1 cut(s)	2268
BglI	GCCNNNNNGGC	1 cut(s)	109
BisI	GCNGC	9 cut(s)	27, 162, 1590, 1641, 1878, 1881, 2370, 2562, 2646
BlsI	GCNGC	9 cut(s)	28, 163, 1591, 1642, 1879, 1882, 2371, 2563, 2647
BmcAI	AGTACT	1 cut(s)	2924
Bme18I	GGWCC	3 cut(s)	1409, 2203, 2398
BmeT110I	CYCGRG	2 cut(s)	382, 1711
BmgT120I	GGNCC	8 cut(s)	1409, 1912, 2203, 2398, 2510, 2643, 2653, 2654
BmiI	GGNNCC	8 cut(s)	185, 648, 2013, 2204, 2205, 2611, 2655, 2963
BmsI	GCATC	5 cut(s)	589, 2275, 2358, 2548, 2755
BpiI	GAAGAC	1 cut(s)	314
BplI	GAGNNNNNCTC	2 cut(s)	1629, 1661
BpmI	CTGGAG	4 cut(s)	202, 1740, 2617, 2939
Bpu10I	CCTNAGC	3 cut(s)	146, 2085, 2966
BpuEI	CTTGAG	2 cut(s)	1013, 2405
BpuMI	CCSGG	4 cut(s)	333, 1712, 1713, 2536
BsaBI	GATNNNNATC	1 cut(s)	2732
BsaI	GGTCTC	1 cut(s)	1411
BsaXI	ACNNNNNCTCC	3 cut(s)	32, 116, 146
Bsc4I	CCNNNNNNNGG	6 cut(s)	332, 338, 815, 1202, 1718, 2640
Bse118I	RCCGGY	1 cut(s)	2756
Bse1I	ACTGG	3 cut(s)	1267, 2791, 2922
Bse21I	CCTNAGG	1 cut(s)	107
Bse3DI	GCAATG	1 cut(s)	2443
Bse8I	GATNNNNATC	1 cut(s)	2732
BseBI	CCWGG	6 cut(s)	810, 1014, 2265, 2455, 2635, 2841
BseGI	GGATG	7 cut(s)	475, 953, 1921, 2038, 2290, 2733, 2770
BseJI	GATNNNNATC	1 cut(s)	2732
BseLI	CCNNNNNNNGG	6 cut(s)	332, 338, 815, 1202, 1718, 2640
BseMI	GCAATG	1 cut(s)	2443
BseMII	CTCAG	8 cut(s)	55, 121, 160, 1088, 1188, 1419, 2076, 2898
BseNI	ACTGG	3 cut(s)	1267, 2791, 2922
BseSI	GKGCMC	3 cut(s)	1805, 2589, 2657
BseXI	GCAGC	7 cut(s)	13, 1576, 1627, 1867, 1889, 2381, 2573
BseYI	CCCAGC	1 cut(s)	2338
BsgI	GTGCAG	3 cut(s)	731, 1817, 2872
BsiHKAI	GWGCWC	4 cut(s)	546, 1805, 1890, 2129
BsiHKCI	CYCGRG	2 cut(s)	382, 1711
BsiSI	CCGG	5 cut(s)	333, 1712, 2535, 2607, 2757
BslFI	GGGAC	2 cut(s)	1177, 2216
BslI	CCNNNNNNNGG	6 cut(s)	332, 338, 815, 1202, 1718, 2640
BsmAI	GTCTC	3 cut(s)	100, 1271, 1411
BsmBI	CGTCTC	1 cut(s)	100
BsmFI	GGGAC	2 cut(s)	1177, 2216
BsmI	GAATGC	1 cut(s)	2940
Bso31I	GGTCTC	1 cut(s)	1411
BsoBI	CYCGRG	2 cut(s)	382, 1711
Bsp120I	GGGCCC	1 cut(s)	2653
Bsp19I	CCATGG	3 cut(s)	282, 1212, 1688
BspCNI	CTCAG	8 cut(s)	54, 120, 159, 1089, 1189, 1418, 2077, 2899
BspHI	TCATGA	2 cut(s)	1423, 2224
BspLI	GGNNCC	8 cut(s)	185, 648, 2013, 2204, 2205, 2611, 2655, 2963
BspMAI	CTGCAG	1 cut(s)	2371
BspMI	ACCTGC	1 cut(s)	2894
BspPI	GGATC	3 cut(s)	732, 820, 1836
BspTI	CTTAAG	1 cut(s)	1565
BspTNI	GGTCTC	1 cut(s)	1411
BsrBI	CCGCTC	3 cut(s)	164, 225, 1749
BsrDI	GCAATG	1 cut(s)	2443
BsrFI	RCCGGY	1 cut(s)	2756
BsrI	ACTGG	3 cut(s)	1267, 2791, 2922
BssAI	RCCGGY	1 cut(s)	2756
BssNAI	GTATAC	1 cut(s)	2802
BssSI	CACGAG	1 cut(s)	537
BssT1I	CCWWGG	4 cut(s)	282, 1212, 1688, 2649
Bst1107I	GTATAC	1 cut(s)	2802
Bst2BI	CACGAG	1 cut(s)	537
Bst2UI	CCWGG	6 cut(s)	810, 1014, 2265, 2455, 2635, 2841
Bst6I	CTCTTC	1 cut(s)	103
BstAFI	CTTAAG	1 cut(s)	1565
BstAPI	GCANNNNNTGC	3 cut(s)	1181, 1809, 2684
BstC8I	GCNNGC	8 cut(s)	166, 534, 714, 992, 1308, 1464, 1557, 2972
BstDSI	CCRYGG	5 cut(s)	187, 282, 1212, 1688, 2932
BstEII	GGTNACC	1 cut(s)	396
BstF5I	GGATG	7 cut(s)	475, 953, 1921, 2038, 2290, 2733, 2770
BstH2I	RGCGCY	1 cut(s)	208
BstHHI	GCGC	2 cut(s)	207, 270
BstMAI	GTCTC	3 cut(s)	100, 1271, 1411
BstNI	CCWGG	6 cut(s)	810, 1014, 2265, 2455, 2635, 2841
BstNSI	RCATGY	2 cut(s)	1466, 1812
BstPI	GGTNACC	1 cut(s)	396
BstSFI	CTRYAG	5 cut(s)	358, 651, 1676, 1742, 2367
BstSLI	GKGCMC	3 cut(s)	1805, 2589, 2657
BstV1I	GCAGC	7 cut(s)	13, 1576, 1627, 1867, 1889, 2381, 2573
BstV2I	GAAGAC	1 cut(s)	314
BstX2I	RGATCY	3 cut(s)	724, 812, 1828
BstYI	RGATCY	3 cut(s)	724, 812, 1828
BstZ17I	GTATAC	1 cut(s)	2802
Bsu36I	CCTNAGG	1 cut(s)	107
BsuI	GTATCC	1 cut(s)	2268
BtgI	CCRYGG	5 cut(s)	187, 282, 1212, 1688, 2932
BtgZI	GCGATG	2 cut(s)	1155, 2693
BtsCI	GGATG	7 cut(s)	475, 953, 1921, 2038, 2290, 2733, 2770
BtsI	GCAGTG	2 cut(s)	714, 2685
BtsIMutI	CAGTG	4 cut(s)	714, 2685, 2889, 2904
BveI	ACCTGC	1 cut(s)	2894
Cac8I	GCNNGC	8 cut(s)	166, 534, 714, 992, 1308, 1464, 1557, 2972
CaiI	CAGNNNCTG	1 cut(s)	1883
CciI	TCATGA	2 cut(s)	1423, 2224
CfoI	GCGC	2 cut(s)	207, 270
Cfr10I	RCCGGY	1 cut(s)	2756
Cfr13I	GGNCC	8 cut(s)	1409, 1912, 2203, 2398, 2510, 2643, 2653, 2654
Cfr9I	CCCGGG	1 cut(s)	1711
CseI	GACGC	1 cut(s)	2032
Csp6I	GTAC	4 cut(s)	2194, 2290, 2377, 2923
CviQI	GTAC	4 cut(s)	2194, 2290, 2377, 2923
EaeI	YGGCCR	4 cut(s)	988, 2063, 2451, 2842
Eam1104I	CTCTTC	1 cut(s)	103
EarI	CTCTTC	1 cut(s)	103
EciI	GGCGGA	2 cut(s)	148, 2465
Eco130I	CCWWGG	4 cut(s)	282, 1212, 1688, 2649
Eco147I	AGGCCT	1 cut(s)	112
Eco24I	GRGCYC	5 cut(s)	58, 1029, 1398, 2232, 2657
Eco31I	GGTCTC	1 cut(s)	1411
Eco32I	GATATC	1 cut(s)	2059
Eco47I	GGWCC	3 cut(s)	1409, 2203, 2398
Eco81I	CCTNAGG	1 cut(s)	107
Eco88I	CYCGRG	2 cut(s)	382, 1711
Eco91I	GGTNACC	1 cut(s)	396
EcoO109I	RGGNCCY	4 cut(s)	1409, 2203, 2510, 2653
EcoO65I	GGTNACC	1 cut(s)	396
EcoRI	GAATTC	3 cut(s)	731, 1130, 1819
EcoRII	CCWGG	6 cut(s)	808, 1012, 2263, 2453, 2633, 2839
EcoRV	GATATC	1 cut(s)	2059
EcoT14I	CCWWGG	4 cut(s)	282, 1212, 1688, 2649
EcoT22I	ATGCAT	1 cut(s)	1814
EcoT38I	GRGCYC	5 cut(s)	58, 1029, 1398, 2232, 2657
ErhI	CCWWGG	4 cut(s)	282, 1212, 1688, 2649
Esp3I	CGTCTC	1 cut(s)	100
FalI	AAGNNNNNCTT	2 cut(s)	1831, 1863
FaqI	GGGAC	2 cut(s)	1177, 2216
FauI	CCCGC	2 cut(s)	1299, 2335
FbaI	TGATCA	4 cut(s)	667, 1231, 1768, 2326
FblI	GTMKAC	4 cut(s)	579, 654, 1675, 2801
Fnu4HI	GCNGC	9 cut(s)	27, 162, 1590, 1641, 1878, 1881, 2370, 2562, 2646
FokI	GGATG	7 cut(s)	482, 960, 1908, 2045, 2297, 2740, 2777
FriOI	GRGCYC	5 cut(s)	58, 1029, 1398, 2232, 2657
Fsp4HI	GCNGC	9 cut(s)	27, 162, 1590, 1641, 1878, 1881, 2370, 2562, 2646
FspBI	CTAG	5 cut(s)	51, 1370, 1662, 2568, 2777
GlaI	GCGC	2 cut(s)	206, 269
GluI	GCNGC	9 cut(s)	27, 162, 1590, 1641, 1878, 1881, 2370, 2562, 2646
GsaI	CCCAGC	1 cut(s)	2342
GsuI	CTGGAG	4 cut(s)	202, 1740, 2617, 2939
HaeII	RGCGCY	1 cut(s)	208
HapII	CCGG	5 cut(s)	333, 1712, 2535, 2607, 2757
HgaI	GACGC	1 cut(s)	2032
HhaI	GCGC	2 cut(s)	207, 270
Hin6I	GCGC	2 cut(s)	205, 268
HinP1I	GCGC	2 cut(s)	205, 268
HincII	GTYRAC	5 cut(s)	580, 685, 1327, 1993, 2485
HindII	GTYRAC	5 cut(s)	580, 685, 1327, 1993, 2485
HindIII	AAGCTT	2 cut(s)	2489, 2877
HinfI	GANTC	9 cut(s)	414, 425, 623, 753, 908, 2135, 2164, 2183, 2703
HpaI	GTTAAC	1 cut(s)	2485
HpaII	CCGG	5 cut(s)	333, 1712, 2535, 2607, 2757
HphI	GGTGA	4 cut(s)	1163, 2321, 2771, 2875
Hpy188I	TCNGA	8 cut(s)	44, 236, 292, 375, 413, 1387, 1456, 1861
Hpy99I	CGWCG	2 cut(s)	245, 2433
HpyCH4IV	ACGT	2 cut(s)	687, 2952
HpySE526I	ACGT	2 cut(s)	687, 2952
HspAI	GCGC	2 cut(s)	205, 268
KflI	GGGWCCC	1 cut(s)	2203
Ksp22I	TGATCA	4 cut(s)	667, 1231, 1768, 2326
KspAI	GTTAAC	1 cut(s)	2485
Lsp1109I	GCAGC	7 cut(s)	13, 1576, 1627, 1867, 1889, 2381, 2573
LweI	GCATC	5 cut(s)	589, 2275, 2358, 2548, 2755
MaeI	CTAG	5 cut(s)	51, 1370, 1662, 2568, 2777
MaeII	ACGT	2 cut(s)	687, 2952
MbiI	CCGCTC	3 cut(s)	164, 225, 1749
MboII	GAAGA	5 cut(s)	90, 314, 643, 1213, 2936
MfeI	CAATTG	1 cut(s)	2016
MflI	RGATCY	3 cut(s)	724, 812, 1828
MlsI	TGGCCA	3 cut(s)	990, 2453, 2844
MluNI	TGGCCA	3 cut(s)	990, 2453, 2844
MlyI	GAGTC	1 cut(s)	2144
MmeI	TCCRAC	4 cut(s)	880, 937, 1096, 1771
Mox20I	TGGCCA	3 cut(s)	990, 2453, 2844
Mph1103I	ATGCAT	1 cut(s)	1814
MroXI	GAANNNNTTC	2 cut(s)	261, 907
MscI	TGGCCA	3 cut(s)	990, 2453, 2844
MseI	TTAA	8 cut(s)	765, 834, 1046, 1566, 1695, 2484, 2673, 2835
MslI	CAYNNNNRTG	5 cut(s)	192, 1428, 1575, 1839, 2909
Msp20I	TGGCCA	3 cut(s)	990, 2453, 2844
MspA1I	CMGCKG	5 cut(s)	26, 77, 572, 1880, 2342
MspCI	CTTAAG	1 cut(s)	1565
MspI	CCGG	5 cut(s)	333, 1712, 2535, 2607, 2757
MunI	CAATTG	1 cut(s)	2016
Mva1269I	GAATGC	1 cut(s)	2940
MvaI	CCWGG	6 cut(s)	810, 1014, 2265, 2455, 2635, 2841
NciI	CCSGG	4 cut(s)	333, 1712, 1713, 2536
NcoI	CCATGG	3 cut(s)	282, 1212, 1688
NlaIV	GGNNCC	8 cut(s)	185, 648, 2013, 2204, 2205, 2611, 2655, 2963
NmeAIII	GCCGAG	1 cut(s)	2638
NmuCI	GTSAC	6 cut(s)	1084, 1432, 2309, 2789, 2851, 2857
NsiI	ATGCAT	1 cut(s)	1814
NspI	RCATGY	2 cut(s)	1466, 1812
OliI	CACNNNNGTG	3 cut(s)	192, 1839, 2909
PaeI	GCATGC	1 cut(s)	1466
PagI	TCATGA	2 cut(s)	1423, 2224
PaqCI	CACCTGC	1 cut(s)	2894
PceI	AGGCCT	1 cut(s)	112
PctI	GAATGC	1 cut(s)	2940
PdmI	GAANNNNTTC	2 cut(s)	261, 907
PfeI	GAWTC	8 cut(s)	414, 425, 623, 753, 908, 2164, 2183, 2703
PflFI	GACNNNGTC	1 cut(s)	1282
PflMI	CCANNNNNTGG	1 cut(s)	2640
PkrI	GCNGC	9 cut(s)	28, 163, 1591, 1642, 1879, 1882, 2371, 2563, 2647
PleI	GAGTC	1 cut(s)	2143
PpsI	GAGTC	1 cut(s)	2143
PpuMI	RGGWCCY	2 cut(s)	1409, 2203
Psp5II	RGGWCCY	2 cut(s)	1409, 2203
Psp6I	CCWGG	6 cut(s)	808, 1012, 2263, 2453, 2633, 2839
PspEI	GGTNACC	1 cut(s)	396
PspFI	CCCAGC	1 cut(s)	2338
PspGI	CCWGG	6 cut(s)	808, 1012, 2263, 2453, 2633, 2839
PspN4I	GGNNCC	8 cut(s)	185, 648, 2013, 2204, 2205, 2611, 2655, 2963
PspOMI	GGGCCC	1 cut(s)	2653
PspPI	GGNCC	8 cut(s)	1409, 1912, 2203, 2398, 2510, 2643, 2653, 2654
PspPPI	RGGWCCY	2 cut(s)	1409, 2203
PsrI	GAACNNNNNNTAC	2 cut(s)	1659, 1691
PstI	CTGCAG	1 cut(s)	2371
PstNI	CAGNNNCTG	1 cut(s)	1883
PsuI	RGATCY	3 cut(s)	724, 812, 1828
PsyI	GACNNNGTC	1 cut(s)	1282
PvuII	CAGCTG	2 cut(s)	572, 1880
RsaI	GTAC	4 cut(s)	2195, 2291, 2378, 2924
RsaNI	GTAC	4 cut(s)	2194, 2290, 2377, 2923
RseI	CAYNNNNRTG	5 cut(s)	192, 1428, 1575, 1839, 2909
SalI	GTCGAC	1 cut(s)	578
SaqAI	TTAA	8 cut(s)	765, 834, 1046, 1566, 1695, 2484, 2673, 2835
SatI	GCNGC	9 cut(s)	27, 162, 1590, 1641, 1878, 1881, 2370, 2562, 2646
Sau96I	GGNCC	8 cut(s)	1409, 1912, 2203, 2398, 2510, 2643, 2653, 2654
ScaI	AGTACT	1 cut(s)	2924
SchI	GAGTC	1 cut(s)	2144
SfaNI	GCATC	5 cut(s)	589, 2275, 2358, 2548, 2755
SfcI	CTRYAG	5 cut(s)	358, 651, 1676, 1742, 2367
SinI	GGWCC	3 cut(s)	1409, 2203, 2398
SmaI	CCCGGG	1 cut(s)	1713
SmiMI	CAYNNNNRTG	5 cut(s)	192, 1428, 1575, 1839, 2909
SmlI	CTYRAG	3 cut(s)	1028, 1565, 2384
SmoI	CTYRAG	3 cut(s)	1028, 1565, 2384
SphI	GCATGC	1 cut(s)	1466
SseBI	AGGCCT	1 cut(s)	112
SspI	AATATT	2 cut(s)	1736, 1786
SspMI	CTAG	5 cut(s)	51, 1370, 1662, 2568, 2777
StuI	AGGCCT	1 cut(s)	112
StyI	CCWWGG	4 cut(s)	282, 1212, 1688, 2649
TaiI	ACGT	2 cut(s)	690, 2955
TaqI	TCGA	7 cut(s)	58, 255, 516, 579, 1392, 1545, 2697
TatI	WGTACW	2 cut(s)	2193, 2922
TauI	GCSGC	2 cut(s)	164, 2648
TfiI	GAWTC	8 cut(s)	414, 425, 623, 753, 908, 2164, 2183, 2703
Tru1I	TTAA	8 cut(s)	765, 834, 1046, 1566, 1695, 2484, 2673, 2835
Tru9I	TTAA	8 cut(s)	765, 834, 1046, 1566, 1695, 2484, 2673, 2835
TscAI	CASTG	4 cut(s)	714, 2685, 2896, 2911
TseFI	GTSAC	6 cut(s)	1084, 1432, 2309, 2789, 2851, 2857
TseI	GCWGC	7 cut(s)	26, 1589, 1640, 1877, 1880, 2369, 2561
Tsp45I	GTSAC	6 cut(s)	1084, 1432, 2309, 2789, 2851, 2857
TspDTI	ATGAA	6 cut(s)	620, 815, 1463, 1910, 2213, 2457
TspGWI	ACGGA	4 cut(s)	2024, 2879, 2886, 2949
TspMI	CCCGGG	1 cut(s)	1711
TspRI	CASTG	4 cut(s)	714, 2685, 2896, 2911
Tth111I	GACNNNGTC	1 cut(s)	1282
Van91I	CCANNNNNTGG	1 cut(s)	2640
Vha464I	CTTAAG	1 cut(s)	1565
VneI	GTGCAC	1 cut(s)	1801
VpaK11BI	GGWCC	3 cut(s)	1409, 2203, 2398
XceI	RCATGY	2 cut(s)	1466, 1812
XmaI	CCCGGG	1 cut(s)	1711
XmiI	GTMKAC	4 cut(s)	579, 654, 1675, 2801
XmnI	GAANNNNTTC	2 cut(s)	261, 907
XspI	CTAG	5 cut(s)	51, 1370, 1662, 2568, 2777
ZrmI	AGTACT	1 cut(s)	2924
Zsp2I	ATGCAT	1 cut(s)	1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.