Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp03

Orthogroup ID

OG0009004

Physical Location & Seq

Genomic Coordinates Forward (+)

21942882 .. 21950028

Gene Structure 7147 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.3G217300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2991 bp

ATGTATATAAACACAGCCTCTTCATCTGCCTCCTCGCTTCTCAGAGCTTCTAGGGCTCGCCTTTTCTCCTCTTCTTCTTCCTCTTCTCCTATCTCTAGAACCTTCGCTGGTTCGCCGCTCAAGCCTTCTCACCATCACTTCGCCTCTCACCGCTTCCTCAGCTCCTCCTCTGCGGTTCGCTCTTTCTCTCGCTGGAGCCACGGCGGTGTCCATTGGCGCTCCCCCTACACGCTTCGGTCTCAGATCAGAGCCGTCGCTCCCGTAATCGAACAGTTCCAGCGCAAGATGGCTTCCATGGCTTCTGAAAACCCTTTCAAGGCAAATTTGACAAGTCTTCCCAAGCCCGGAGGTGGTGAGTTTGGAAAATTCTACAGCCTTCCCTCTCTGAACGACCCCAGGATCGATAGGTTACCATACTCCATCAGAATCCTTCTTGAATCTGCTATTCGTAATTGTGACAATTTCCAAGTCAAGAAGGAGGATGTTGAGAAAATTCTTGATTGGGAAAAGACTGCCCCGAAGCAAGTTGAAATTCCTTTCAAGCCTGCTCGTGTACTATTGCAGGACTTTACGGGAGTTCCAGCTGTGGTCGATCTTGCTTGTATGCGTGATGCTATGAACAAACTTGGGAGCGATTCCGACAAGATCAATCCTTTGGTTCCTGTTGATCTTGTTATTGATCATTCAGTTCAAGTCGACGTTGCAGGATCAGCAAATGCCGTGCAGGCCAATATGGACCTTGAATTCCAGAGAAACAGAGAGAGATTCGCTTTTCTTAAATGGGGGTCCAATGCTTTCCACAATATGCTTGTTGTTCCGCCTGGTTCTGGCATTGTTCATCAGGTTAATCTTGAATATCTTGGACGGGTTGTTTTCAACACTGATGGCTTACTCTACCCTGATAGCGTGGTTGGAACTGATTCCCACACAACCATGATTGATGGGTTAGGAGTTGCTGGCTGGGGAGTTGGAGGTATTGAAGCTGAAGCTACTATGCTTGGCCAGCCTATGAGCATGGTGTTGCCTGGAGTTGTTGGGTTCAAGTTATCTGGAAAATTAAACAATGGTGTTACTGCTACTGACTTGGTTTTGACTGTCACACAAATACTGAGGAAGCATGGAGTTGTTGGAAAGTTTGTTGAATTCTATGGGGAAGGTATGGGTGAGCTATCATTAGCTGACAGGGCCACTATTGCCAATATGTCCCCTGAATATGGTGCAACCATGGGGTTCTTCCCTGTAGACCATGTTACTTTACAGTATCTCAAATTGACTGGTAGAAGTGAGGAGACTGTGTCAATGATTGAAAGTTATCTCCGGGCAAATAAATTGTTTGTTGACTATAATGAGCCTCAGTCAGAAAGAGTGTATTCATCATACCTAGAATTGAACCTTTCGGAAGTCGAGCCCTGTATGTCTGGACCTAAGAGACCTCACGATCGTGTGCCTTTGAAAGACATGAAAGTTGATTGGCATGCATGTCTTGACAACAAGGTTGGGTTCAAGGGATTTGCTATACCAAAAGAGGTGCAAGACAAGGTGGCGAAGTTTTCATTCCATGGACAGCCGGCAGAGCTTAAGCATGGTAGTGTTGTGATTGCTGCAATCACAAGCTGCACCAATACATCAAACCCAAGTGTCATGCTTGGGGCTGCCCTTGTTGCAAAAAAGGCTAGTGAACTTGGTCTACAGGTTAAGCCATGGGTGAAAACTAGTCTTGCCCCGGGTTCCGGAGTTGTTACAAAATATTTGCTCAAGAGTGGATTGCAAAAGTATTTTGATCAACAAGGTTTTCATATTGTTGGATATGGGTGCACAACATGCATTGGGAATTCAGGGGACTTAGATGAAACTGTTGCTTCTGCTATAGCAGAAAATGACATTGTTGCAGCTGCTGTGCTCTCTGGAAACAGAAATTTTGAGGGTCGTGTTCATCCATTGACAAGAGCTAATTACCTTGCCTCTCCTCCTTTAGTGGTTGCTTATGCCCTTGCTGGAACAGTTGACATTGACTTTGATAAGGAGCCAATTGGAACAGGGAAGGATGGTAAGAGCGTTTATTTCAGGGATATCTGGCCATCTACTGAGGAGATTGCGGAGGTAGTACAATCAAGTGTGTTGCCAGATATGTTCAGAAGCACTTATGAGTCTATTACTAAGGGCAACCCCACGTGGAATGAGCTATCAGTTACAGATTCCAAACTTTACTCTTGGGACCCTAATTCAACCTACATTCATGAGCCCCCATATTTCAAGGGTATGACCATGGATCCACCTGGCGCAAAGGGAGTGAAGGATGCATACTGCCTGTTGAATTTTGGTGACAGTATTACAACAGATCACATCTCCCCAGCAGGAAGCATCCACAAGGACAGTCCTGCAGCAAAATACCTTCTTGAGCGTGGGGTGGATCGCAAAGACTTCAACTCTTACGGTAGTCGTCGTGGCAATGATGAAGTCATGGCAAGGGGAACCTTTGCCAATATCCGCATTGTTAACAAGCTTTTGAATGGAGAAGTGGGCCCTAAAACAGTTCACATTCCTACAGGAGAGAAACTCTATGTGTTTGATGCAGCAACAAGATACAAGGCTGATGGGCATGACACCATTGTACTGGCTGGAGCTGAGTATGGAAGTGGAAGCTCTAGGGATTGGGCTGCCAAGGGTCCAATGCTATTGGGTGTTAAAGCGGTTATTGCTAAAAGTTTCGAGAGAATCCATCGTAGTAATTTGGTGGGGATGGGTATTGTTCCACTTTGTTTCAAGGCCGGCGAGGATGCTGATACACTAGGATTGACTGGTCATGAACGTTATACCATCGACCTCCCTAGCAGCATTAGTGAGATAAAACCCGGCCAAGATGTGACTGTCACTACAGACAATGGGAAATCTTTCACCTGCACTGTACGCTTTGACACTGAGGTGGAATTGGCTTATTTCAACCACGGAGGTATTCTTCATTATGTGATCAGGAACCTGAGTAAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031090 GO:0031667 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048856 GO:0050896 GO:0071704 GO:0071944 GO:0072350 GO:0090351 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

997

Amino Acids

108.7

Weight (kDa)

6.98

Isoelectric Point (pI)

38.0

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05710
fragaria_vesca	FvH4_6g43190
malus_domestica	MD09G1108800.v1.1 MD17G1096700.v1.1
prunus_persica	Prupe.3G217300_v2.0.a1 Prupe.3G217300_v2.0.a1
pyrus_communis	pycom09g03120 pycom17g09260
rosa_chinensis	RchiOBHm_Chr2g0159291
rosa_laevigata	RLG00000021152
rosa_multiflora	Rmu_sc0004800.1_g000001 Rmu_sc0007806.1_g000010 Rmu_sc0010590.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090620
rosa_rugosa	Rorug02G0475700
rosa_samantha	Rh2AG542100 Rh2BG554700 Rh2CG524800 Rh2DG564400 Rh5AG522200
rosa_wichuraiana	Rw2G044840 Rw5G048460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2906
Acc36I	ACCTGC	1 cut(s)	2906
AccBSI	CCGCTC	1 cut(s)	118
AccI	GTMKAC	3 cut(s)	696, 1242, 1687
AccIII	TCCGGA	1 cut(s)	1730
AciI	CCGC	8 cut(s)	116, 151, 173, 204, 818, 2096, 2488, 2690
AclI	AACGTT	1 cut(s)	2809
AclWI	GGATC	5 cut(s)	407, 715, 2264, 2277, 2418
AcoI	YGGCCR	3 cut(s)	1000, 2075, 2854
AcsI	RAATTY	9 cut(s)	322, 365, 492, 531, 743, 1142, 1831, 1915, 2314
AcuI	CTGAAG	1 cut(s)	1005
AcvI	CACGTG	1 cut(s)	2172
AfaI	GTAC	4 cut(s)	555, 2106, 2613, 2907
AfiI	CCNNNNNNNGG	6 cut(s)	316, 344, 350, 827, 1214, 1730
AflII	CTTAAG	1 cut(s)	1577
AhlI	ACTAGT	1 cut(s)	1712
AjnI	CCWGG	4 cut(s)	395, 820, 1024, 2275
AleI	CACNNNNGTG	3 cut(s)	204, 1440, 2921
Alw21I	GWGCWC	2 cut(s)	1817, 1902
Alw26I	GTCTC	3 cut(s)	243, 1283, 1423
Alw44I	GTGCAC	1 cut(s)	1813
AlwI	GGATC	5 cut(s)	407, 715, 2264, 2277, 2418
AlwNI	CAGNNNCTG	1 cut(s)	1895
Ama87I	CYCGRG	1 cut(s)	1723
Aor13HI	TCCGGA	1 cut(s)	1730
AoxI	GGCC	7 cut(s)	726, 1000, 1185, 2075, 2521, 2766, 2854
ApaI	GGGCCC	1 cut(s)	2525
ApaLI	GTGCAC	1 cut(s)	1813
ApeKI	GCWGC	9 cut(s)	1601, 1614, 1652, 1889, 1892, 2381, 2573, 2657, 2832
ApoI	RAATTY	9 cut(s)	322, 365, 492, 531, 743, 1142, 1831, 1915, 2314
ArsI	GACNNNNNNTTYG	2 cut(s)	1997, 2029
Asp700I	GAANNNNTTC	2 cut(s)	919, 2891
AspLEI	GCGC	3 cut(s)	219, 282, 2282
AspS9I	GGNCC	8 cut(s)	736, 786, 1185, 1421, 2215, 2521, 2522, 2666
AsuC2I	CCSGG	5 cut(s)	345, 1319, 1724, 1725, 2853
AsuHPI	GGTGA	7 cut(s)	122, 140, 365, 1175, 1717, 2333, 2887
AvaI	CYCGRG	1 cut(s)	1723
AvaII	GGWCC	5 cut(s)	736, 786, 1421, 2215, 2666
BaeGI	GKGCMC	2 cut(s)	1817, 2525
BalI	TGGCCA	2 cut(s)	1002, 2077
BamHI	GGATCC	1 cut(s)	2269
BanII	GRGCYC	4 cut(s)	58, 1410, 2244, 2525
BarI	GAAGNNNNNNTAC	2 cut(s)	2285, 2317
BauI	CACGAG	1 cut(s)	549
BbrPI	CACGTG	1 cut(s)	2172
BbsI	GAAGAC	1 cut(s)	326
Bbv12I	GWGCWC	2 cut(s)	1817, 1902
BbvCI	CCTCAGC	1 cut(s)	158
BbvI	GCAGC	9 cut(s)	1588, 1601, 1639, 1879, 1901, 2393, 2585, 2644, 2844
BceAI	ACGGC	3 cut(s)	217, 236, 704
BciT130I	CCWGG	4 cut(s)	397, 822, 1026, 2277
BclI	TGATCA	3 cut(s)	679, 1780, 2967
BcnI	CCSGG	5 cut(s)	345, 1319, 1724, 1725, 2853
BcoDI	GTCTC	3 cut(s)	243, 1283, 1423
BcuI	ACTAGT	1 cut(s)	1712
BfaI	CTAG	8 cut(s)	51, 96, 1382, 1674, 1713, 2646, 2789, 2829
BfmI	CTRYAG	7 cut(s)	370, 1239, 1688, 1866, 2379, 2544, 2874
BfoI	RGCGCY	1 cut(s)	220
BfrI	CTTAAG	1 cut(s)	1577
BfuAI	ACCTGC	1 cut(s)	2906
BglI	GCCNNNNNGGC	1 cut(s)	725
Bme1390I	CCNGG	9 cut(s)	345, 397, 822, 1026, 1319, 1724, 1725, 2277, 2853
Bme18I	GGWCC	5 cut(s)	736, 786, 1421, 2215, 2666
BmeT110I	CYCGRG	1 cut(s)	1723
BmgT120I	GGNCC	8 cut(s)	736, 786, 1185, 1421, 2215, 2521, 2522, 2666
BmrFI	CCNGG	9 cut(s)	345, 397, 822, 1026, 1319, 1724, 1725, 2277, 2853
BmsI	GCATC	5 cut(s)	601, 2287, 2370, 2560, 2767
BpiI	GAAGAC	1 cut(s)	326
BplI	GAGNNNNNCTC	2 cut(s)	2541, 2573
BpmI	CTGGAG	3 cut(s)	214, 1047, 2640
Bpu10I	CCTNAGC	1 cut(s)	158
BpuEI	CTTGAG	3 cut(s)	104, 1739, 2417
BpuMI	CCSGG	5 cut(s)	345, 1319, 1724, 1725, 2853
Bsa29I	ATCGAT	1 cut(s)	402
BsaAI	YACGTR	1 cut(s)	2172
BsaI	GGTCTC	2 cut(s)	243, 1423
BsaWI	WCCGGW	1 cut(s)	1730
BsaXI	ACNNNNNCTCC	2 cut(s)	1280, 1310
Bsc4I	CCNNNNNNNGG	6 cut(s)	316, 344, 350, 827, 1214, 1730
Bse118I	RCCGGY	2 cut(s)	1567, 2768
Bse1I	ACTGG	3 cut(s)	1279, 2619, 2803
Bse3DI	GCAATG	1 cut(s)	2455
BseAI	TCCGGA	1 cut(s)	1730
BseBI	CCWGG	4 cut(s)	397, 822, 1026, 2277
BseCI	ATCGAT	1 cut(s)	402
BseGI	GGATG	7 cut(s)	487, 1933, 2050, 2302, 2361, 2745, 2782
BseLI	CCNNNNNNNGG	6 cut(s)	316, 344, 350, 827, 1214, 1730
BseMI	GCAATG	1 cut(s)	2455
BseMII	CTCAG	9 cut(s)	55, 172, 254, 1100, 1367, 2076, 2616, 2910, 2969
BseNI	ACTGG	3 cut(s)	1279, 2619, 2803
BseRI	GAGGAG	7 cut(s)	22, 58, 154, 157, 1301, 1956, 2102
BseSI	GKGCMC	2 cut(s)	1817, 2525
BseXI	GCAGC	9 cut(s)	1588, 1601, 1639, 1879, 1901, 2393, 2585, 2644, 2844
BseYI	CCCAGC	2 cut(s)	960, 2350
BsgI	GTGCAG	3 cut(s)	743, 1600, 2884
Bsh1285I	CGRYCG	1 cut(s)	1441
BshFI	GGCC	7 cut(s)	728, 1002, 1187, 2077, 2523, 2768, 2856
BshVI	ATCGAT	1 cut(s)	402
BsiEI	CGRYCG	1 cut(s)	1441
BsiHKAI	GWGCWC	2 cut(s)	1817, 1902
BsiHKCI	CYCGRG	1 cut(s)	1723
BsiSI	CCGG	7 cut(s)	345, 1318, 1568, 1724, 1731, 2769, 2853
BslFI	GGGAC	3 cut(s)	1189, 1853, 2228
BslI	CCNNNNNNNGG	6 cut(s)	316, 344, 350, 827, 1214, 1730
BsmAI	GTCTC	3 cut(s)	243, 1283, 1423
BsmFI	GGGAC	3 cut(s)	1189, 1853, 2228
BsnI	GGCC	7 cut(s)	728, 1002, 1187, 2077, 2523, 2768, 2856
Bso31I	GGTCTC	2 cut(s)	243, 1423
BsoBI	CYCGRG	1 cut(s)	1723
Bsp120I	GGGCCC	1 cut(s)	2521
Bsp1286I	GDGCHC	6 cut(s)	58, 1410, 1817, 1902, 2244, 2525
Bsp13I	TCCGGA	1 cut(s)	1730
Bsp19I	CCATGG	5 cut(s)	294, 1224, 1558, 1700, 2265
BspACI	CCGC	8 cut(s)	116, 151, 173, 204, 818, 2096, 2488, 2690
BspANI	GGCC	7 cut(s)	728, 1002, 1187, 2077, 2523, 2768, 2856
BspCNI	CTCAG	9 cut(s)	54, 171, 253, 1101, 1366, 2077, 2617, 2911, 2970
BspDI	ATCGAT	1 cut(s)	402
BspEI	TCCGGA	1 cut(s)	1730
BspHI	TCATGA	2 cut(s)	2236, 2803
BspMAI	CTGCAG	1 cut(s)	2383
BspMI	ACCTGC	1 cut(s)	2906
BspPI	GGATC	5 cut(s)	407, 715, 2264, 2277, 2418
BspTI	CTTAAG	1 cut(s)	1577
BspTNI	GGTCTC	2 cut(s)	243, 1423
BsrBI	CCGCTC	1 cut(s)	118
BsrDI	GCAATG	1 cut(s)	2455
BsrFI	RCCGGY	2 cut(s)	1567, 2768
BsrI	ACTGG	3 cut(s)	1279, 2619, 2803
BssAI	RCCGGY	2 cut(s)	1567, 2768
BssSI	CACGAG	1 cut(s)	549
BssT1I	CCWWGG	6 cut(s)	294, 1224, 1558, 1700, 2265, 2661
Bst2BI	CACGAG	1 cut(s)	549
Bst2UI	CCWGG	4 cut(s)	397, 822, 1026, 2277
Bst6I	CTCTTC	3 cut(s)	25, 76, 88
BstAFI	CTTAAG	1 cut(s)	1577
BstAPI	GCANNNNNTGC	1 cut(s)	1821
BstBAI	YACGTR	1 cut(s)	2172
BstC8I	GCNNGC	8 cut(s)	58, 546, 726, 958, 1004, 1476, 1569, 2770
BstDSI	CCRYGG	7 cut(s)	199, 294, 1224, 1558, 1700, 2265, 2944
BstEII	GGTNACC	1 cut(s)	408
BstF5I	GGATG	7 cut(s)	487, 1933, 2050, 2302, 2361, 2745, 2782
BstH2I	RGCGCY	1 cut(s)	220
BstHHI	GCGC	3 cut(s)	219, 282, 2282
BstMAI	GTCTC	3 cut(s)	243, 1283, 1423
BstMCI	CGRYCG	1 cut(s)	1441
BstNI	CCWGG	4 cut(s)	397, 822, 1026, 2277
BstNSI	RCATGY	3 cut(s)	1478, 1482, 1824
BstPI	GGTNACC	1 cut(s)	408
BstSCI	CCNGG	9 cut(s)	343, 395, 820, 1024, 1317, 1722, 1723, 2275, 2851
BstSFI	CTRYAG	7 cut(s)	370, 1239, 1688, 1866, 2379, 2544, 2874
BstSLI	GKGCMC	2 cut(s)	1817, 2525
BstV1I	GCAGC	9 cut(s)	1588, 1601, 1639, 1879, 1901, 2393, 2585, 2644, 2844
BstV2I	GAAGAC	1 cut(s)	326
BstX2I	RGATCY	1 cut(s)	2269
BstXI	CCANNNNNNTGG	1 cut(s)	2614
BstYI	RGATCY	1 cut(s)	2269
Bsu15I	ATCGAT	1 cut(s)	402
BsuRI	GGCC	7 cut(s)	728, 1002, 1187, 2077, 2523, 2768, 2856
BsuTUI	ATCGAT	1 cut(s)	402
BtgI	CCRYGG	7 cut(s)	199, 294, 1224, 1558, 1700, 2265, 2944
BtsCI	GGATG	7 cut(s)	487, 1933, 2050, 2302, 2361, 2745, 2782
BtsIMutI	CAGTG	3 cut(s)	879, 2901, 2916
BveI	ACCTGC	1 cut(s)	2906
Cac8I	GCNNGC	8 cut(s)	58, 546, 726, 958, 1004, 1476, 1569, 2770
CaiI	CAGNNNCTG	1 cut(s)	1895
CciI	TCATGA	2 cut(s)	2236, 2803
CfoI	GCGC	3 cut(s)	219, 282, 2282
Cfr10I	RCCGGY	2 cut(s)	1567, 2768
Cfr13I	GGNCC	8 cut(s)	736, 786, 1185, 1421, 2215, 2521, 2522, 2666
Cfr9I	CCCGGG	1 cut(s)	1723
ClaI	ATCGAT	1 cut(s)	402
Csp6I	GTAC	4 cut(s)	554, 2105, 2612, 2906
CviQI	GTAC	4 cut(s)	554, 2105, 2612, 2906
EaeI	YGGCCR	3 cut(s)	1000, 2075, 2854
Eam1104I	CTCTTC	3 cut(s)	25, 76, 88
EarI	CTCTTC	3 cut(s)	25, 76, 88
EciI	GGCGGA	1 cut(s)	807
Eco130I	CCWWGG	6 cut(s)	294, 1224, 1558, 1700, 2265, 2661
Eco24I	GRGCYC	4 cut(s)	58, 1410, 2244, 2525
Eco31I	GGTCTC	2 cut(s)	243, 1423
Eco32I	GATATC	1 cut(s)	2071
Eco47I	GGWCC	5 cut(s)	736, 786, 1421, 2215, 2666
Eco57I	CTGAAG	1 cut(s)	1005
Eco72I	CACGTG	1 cut(s)	2172
Eco88I	CYCGRG	1 cut(s)	1723
Eco91I	GGTNACC	1 cut(s)	408
EcoO109I	RGGNCCY	2 cut(s)	2215, 2522
EcoO65I	GGTNACC	1 cut(s)	408
EcoRI	GAATTC	3 cut(s)	743, 1142, 1831
EcoRII	CCWGG	4 cut(s)	395, 820, 1024, 2275
EcoRV	GATATC	1 cut(s)	2071
EcoT14I	CCWWGG	6 cut(s)	294, 1224, 1558, 1700, 2265, 2661
EcoT22I	ATGCAT	3 cut(s)	1480, 1826, 2302
EcoT38I	GRGCYC	4 cut(s)	58, 1410, 2244, 2525
ErhI	CCWWGG	6 cut(s)	294, 1224, 1558, 1700, 2265, 2661
FaqI	GGGAC	3 cut(s)	1189, 1853, 2228
FbaI	TGATCA	3 cut(s)	679, 1780, 2967
FblI	GTMKAC	3 cut(s)	696, 1242, 1687
FokI	GGATG	7 cut(s)	494, 1920, 2057, 2309, 2348, 2752, 2789
FriOI	GRGCYC	4 cut(s)	58, 1410, 2244, 2525
FspBI	CTAG	8 cut(s)	51, 96, 1382, 1674, 1713, 2646, 2789, 2829
GlaI	GCGC	3 cut(s)	218, 281, 2281
GsaI	CCCAGC	2 cut(s)	964, 2354
GsuI	CTGGAG	3 cut(s)	214, 1047, 2640
HaeII	RGCGCY	1 cut(s)	220
HaeIII	GGCC	7 cut(s)	728, 1002, 1187, 2077, 2523, 2768, 2856
HapII	CCGG	7 cut(s)	345, 1318, 1568, 1724, 1731, 2769, 2853
HhaI	GCGC	3 cut(s)	219, 282, 2282
Hin6I	GCGC	3 cut(s)	217, 280, 2280
HinP1I	GCGC	3 cut(s)	217, 280, 2280
HincII	GTYRAC	4 cut(s)	697, 1339, 2005, 2497
HindII	GTYRAC	4 cut(s)	697, 1339, 2005, 2497
HindIII	AAGCTT	1 cut(s)	2501
HinfI	GANTC	8 cut(s)	426, 437, 635, 765, 920, 2147, 2195, 2715
HpaI	GTTAAC	1 cut(s)	2497
HpaII	CCGG	7 cut(s)	345, 1318, 1568, 1724, 1731, 2769, 2853
HphI	GGTGA	7 cut(s)	122, 140, 365, 1175, 1717, 2333, 2887
Hpy99I	CGWCG	3 cut(s)	257, 701, 2445
HpyAV	CCTTC	9 cut(s)	112, 135, 386, 440, 469, 1148, 2035, 2287, 2402
HpyCH4IV	ACGT	3 cut(s)	699, 2171, 2809
HpySE526I	ACGT	3 cut(s)	699, 2171, 2809
HspAI	GCGC	3 cut(s)	217, 280, 2280
KflI	GGGWCCC	1 cut(s)	2215
Kpn2I	TCCGGA	1 cut(s)	1730
KroI	GCCGGC	2 cut(s)	1567, 2768
KroNI	GCCGGC	2 cut(s)	1569, 2770
Ksp22I	TGATCA	3 cut(s)	679, 1780, 2967
KspAI	GTTAAC	1 cut(s)	2497
LmnI	GCTCC	7 cut(s)	167, 195, 224, 262, 630, 2023, 2621
Lsp1109I	GCAGC	9 cut(s)	1588, 1601, 1639, 1879, 1901, 2393, 2585, 2644, 2844
LweI	GCATC	5 cut(s)	601, 2287, 2370, 2560, 2767
MaeI	CTAG	8 cut(s)	51, 96, 1382, 1674, 1713, 2646, 2789, 2829
MaeII	ACGT	3 cut(s)	699, 2171, 2809
MbiI	CCGCTC	1 cut(s)	118
MboII	GAAGA	8 cut(s)	12, 63, 66, 69, 75, 326, 1225, 2948
MfeI	CAATTG	1 cut(s)	2028
MflI	RGATCY	1 cut(s)	2269
MhlI	GDGCHC	6 cut(s)	58, 1410, 1817, 1902, 2244, 2525
MlsI	TGGCCA	2 cut(s)	1002, 2077
MluNI	TGGCCA	2 cut(s)	1002, 2077
MlyI	GAGTC	1 cut(s)	2156
MmeI	TCCRAC	5 cut(s)	663, 892, 949, 1108, 1783
Mox20I	TGGCCA	2 cut(s)	1002, 2077
Mph1103I	ATGCAT	3 cut(s)	1480, 1826, 2302
MroI	TCCGGA	1 cut(s)	1730
MroNI	GCCGGC	2 cut(s)	1567, 2768
MroXI	GAANNNNTTC	2 cut(s)	919, 2891
MscI	TGGCCA	2 cut(s)	1002, 2077
MseI	TTAA	7 cut(s)	777, 846, 1058, 1578, 1695, 2496, 2685
MslI	CAYNNNNRTG	5 cut(s)	204, 932, 1440, 1587, 2921
Msp20I	TGGCCA	2 cut(s)	1002, 2077
MspA1I	CMGCKG	2 cut(s)	584, 1892
MspCI	CTTAAG	1 cut(s)	1577
MspI	CCGG	7 cut(s)	345, 1318, 1568, 1724, 1731, 2769, 2853
MspR9I	CCNGG	9 cut(s)	345, 397, 822, 1026, 1319, 1724, 1725, 2277, 2853
MunI	CAATTG	1 cut(s)	2028
MvaI	CCWGG	4 cut(s)	397, 822, 1026, 2277
NaeI	GCCGGC	2 cut(s)	1569, 2770
NciI	CCSGG	5 cut(s)	345, 1319, 1724, 1725, 2853
NcoI	CCATGG	5 cut(s)	294, 1224, 1558, 1700, 2265
NgoMIV	GCCGGC	2 cut(s)	1567, 2768
NmuCI	GTSAC	5 cut(s)	455, 1096, 2321, 2863, 2869
NsiI	ATGCAT	3 cut(s)	1480, 1826, 2302
NspI	RCATGY	3 cut(s)	1478, 1482, 1824
OliI	CACNNNNGTG	3 cut(s)	204, 1440, 2921
PaeI	GCATGC	1 cut(s)	1478
PagI	TCATGA	2 cut(s)	2236, 2803
PaqCI	CACCTGC	1 cut(s)	2906
PdiI	GCCGGC	2 cut(s)	1569, 2770
PdmI	GAANNNNTTC	2 cut(s)	919, 2891
PfeI	GAWTC	7 cut(s)	426, 437, 635, 765, 920, 2195, 2715
PflFI	GACNNNGTC	1 cut(s)	1294
Ple19I	CGATCG	1 cut(s)	1441
PleI	GAGTC	1 cut(s)	2155
PmaCI	CACGTG	1 cut(s)	2172
PmlI	CACGTG	1 cut(s)	2172
PpsI	GAGTC	1 cut(s)	2155
Ppu21I	YACGTR	1 cut(s)	2172
PpuMI	RGGWCCY	1 cut(s)	2215
Psp1406I	AACGTT	1 cut(s)	2809
Psp5II	RGGWCCY	1 cut(s)	2215
Psp6I	CCWGG	4 cut(s)	395, 820, 1024, 2275
PspCI	CACGTG	1 cut(s)	2172
PspEI	GGTNACC	1 cut(s)	408
PspFI	CCCAGC	2 cut(s)	960, 2350
PspGI	CCWGG	4 cut(s)	395, 820, 1024, 2275
PspOMI	GGGCCC	1 cut(s)	2521
PspPI	GGNCC	8 cut(s)	736, 786, 1185, 1421, 2215, 2521, 2522, 2666
PspPPI	RGGWCCY	1 cut(s)	2215
PsrI	GAACNNNNNNTAC	2 cut(s)	1671, 1703
PstI	CTGCAG	1 cut(s)	2383
PstNI	CAGNNNCTG	1 cut(s)	1895
PsuI	RGATCY	1 cut(s)	2269
PsyI	GACNNNGTC	1 cut(s)	1294
PvuI	CGATCG	1 cut(s)	1441
PvuII	CAGCTG	2 cut(s)	584, 1892
RsaI	GTAC	4 cut(s)	555, 2106, 2613, 2907
RsaNI	GTAC	4 cut(s)	554, 2105, 2612, 2906
RseI	CAYNNNNRTG	5 cut(s)	204, 932, 1440, 1587, 2921
SalI	GTCGAC	1 cut(s)	695
SaqAI	TTAA	7 cut(s)	777, 846, 1058, 1578, 1695, 2496, 2685
Sau96I	GGNCC	8 cut(s)	736, 786, 1185, 1421, 2215, 2521, 2522, 2666
SchI	GAGTC	1 cut(s)	2156
ScrFI	CCNGG	9 cut(s)	345, 397, 822, 1026, 1319, 1724, 1725, 2277, 2853
SduI	GDGCHC	6 cut(s)	58, 1410, 1817, 1902, 2244, 2525
SfaNI	GCATC	5 cut(s)	601, 2287, 2370, 2560, 2767
SfcI	CTRYAG	7 cut(s)	370, 1239, 1688, 1866, 2379, 2544, 2874
SinI	GGWCC	5 cut(s)	736, 786, 1421, 2215, 2666
SmaI	CCCGGG	1 cut(s)	1725
SmiMI	CAYNNNNRTG	5 cut(s)	204, 932, 1440, 1587, 2921
SmlI	CTYRAG	4 cut(s)	119, 1577, 1754, 2396
SmoI	CTYRAG	4 cut(s)	119, 1577, 1754, 2396
SpeI	ACTAGT	1 cut(s)	1712
SphI	GCATGC	1 cut(s)	1478
SsiI	CCGC	8 cut(s)	116, 151, 173, 204, 818, 2096, 2488, 2690
SspI	AATATT	1 cut(s)	1748
SspMI	CTAG	8 cut(s)	51, 96, 1382, 1674, 1713, 2646, 2789, 2829
StyD4I	CCNGG	9 cut(s)	343, 395, 820, 1024, 1317, 1722, 1723, 2275, 2851
StyI	CCWWGG	6 cut(s)	294, 1224, 1558, 1700, 2265, 2661
TaiI	ACGT	3 cut(s)	702, 2174, 2812
TaqI	TCGA	7 cut(s)	267, 402, 591, 696, 1404, 2709, 2820
TaqII	GACCGA	1 cut(s)	225
TatI	WGTACW	3 cut(s)	553, 2104, 2611
TauI	GCSGC	1 cut(s)	118
TfiI	GAWTC	7 cut(s)	426, 437, 635, 765, 920, 2195, 2715
Tru1I	TTAA	7 cut(s)	777, 846, 1058, 1578, 1695, 2496, 2685
Tru9I	TTAA	7 cut(s)	777, 846, 1058, 1578, 1695, 2496, 2685
TscAI	CASTG	3 cut(s)	886, 2908, 2923
TseFI	GTSAC	5 cut(s)	455, 1096, 2321, 2863, 2869
TseI	GCWGC	9 cut(s)	1601, 1614, 1652, 1889, 1892, 2381, 2573, 2657, 2832
Tsp45I	GTSAC	5 cut(s)	455, 1096, 2321, 2863, 2869
TspGWI	ACGGA	1 cut(s)	2961
TspMI	CCCGGG	1 cut(s)	1723
TspRI	CASTG	3 cut(s)	886, 2908, 2923
Tth111I	GACNNNGTC	1 cut(s)	1294
Vha464I	CTTAAG	1 cut(s)	1577
VneI	GTGCAC	1 cut(s)	1813
VpaK11BI	GGWCC	5 cut(s)	736, 786, 1421, 2215, 2666
XapI	RAATTY	9 cut(s)	322, 365, 492, 531, 743, 1142, 1831, 1915, 2314
XbaI	TCTAGA	1 cut(s)	95
XceI	RCATGY	3 cut(s)	1478, 1482, 1824
XmaI	CCCGGG	1 cut(s)	1723
XmiI	GTMKAC	3 cut(s)	696, 1242, 1687
XmnI	GAANNNNTTC	2 cut(s)	919, 2891
XspI	CTAG	8 cut(s)	51, 96, 1382, 1674, 1713, 2646, 2789, 2829
Zsp2I	ATGCAT	3 cut(s)	1480, 1826, 2302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.