Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009004

Physical Location & Seq

Genomic Coordinates Forward (+)

71579675 .. 71587166

Gene Structure 7492 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G044840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2973 bp

ATGTATATAGCCACAGCCTCTTCCTCTGCCTCGGCTCTCCTCAGAGCTTCTAGGGCACGGTTCTTCTCCTCCTCCACTTCCACCTTGTCCAGAACCTTCTTGGCCGCGCCGCCCAAGCCTTCGTCGCATAGCTTCACCGCTCAGAGGCTTCTCAGCTCCTCGGCCGCCACTCGGTGGAGCCACGGCGTCCATTGGCGCGCTCCCTACACGCTTCGCTCTCAGATCAGAGCCATCGCTCCCGTCCTCGAACGCGTCCAGCGCAGGATCGCTACCATGGCTCCTGAGAGTCATCCATTCAAGGAGCATTTGACAACTCTTCCCAAGCCAGGAGGCGGCGAGTATGGGAAGTTCTATAGCCTTACTTCACTGAAAGATCCGAGGATCGATAAGTTACCGTACTCTATCAGAATCCTCCTTGAATCTGCTATTCGTAATTGTGACAACTTCCAAGTAAAGAAGGAAGATGTTGAGAAAATTCTTGACTGGGAAAATACTGCTCCAAAGCAAGTTGAAATTCCTTTCAAGCCTGCTCGTGTACTCTTGCAGGACTTTACAGGAGTGCCAGCTGTGGTTGACCTTGCTGTAATGCGTGATGCTATGAACGATCTTGGCAGTGATTCTAACAAGATCAATCCCTTGGTGCCTGTTGATCTTGTTGTTGATCACTCCGTTCAAGTCGATGTTGCAAGATCAGAAAATGCAGTGCAGGCAAATATGGAGCTAGAATTCCAGAGAAACCGGGAGAGATTTGCTTTTCTTAAATGGGGTTCTACTGCTTTCCAAAATATGCTTGTTGTTCCACCTGGTTCTGGCATTGTTCATCAGGTCAATCTTGAATATCTTGGACGGGTTGTTTTCAACACTAATGGCTTGCTGTACCCCGACAGTGTGGTTGGAACTGATTCCCATACAACCATGATTGATGGTTTGGGAGTAGCTGGCTGGGGAGTTGGGGGTATTGAAGCAGAAGCTACTATGCTTGGTCAGCCCATGAGCATGGTGTTGCCTGGAGTTGTTGGGTTCAAGTTATCTGGAAAGTTGAACAATGGTGTTACTGCTACTGACTTGGTCTTGACTGTCACACAAATGTTGAGGAAGCATGGAGTTGTGGGAAAATTTGTTGAGTTTTATGGGGGTGGTATGGGTGAACTGTCGTTAGCCGACAGGGCTACTATTGCCAATATGTCTCCTGAATATGGTGCAACCATGGGGTTCTTCCCAGTAGATCATGTTACTTTACAATATCTCAAATTGACTGGCAGAAGCGATGAAACTGTGGCTATGATTGAAGGTTATCTTCGGGCAAATAAATTGTTTGTTGACTATAATGAGCCTGAATCAGAAAGAGTTTACTCATCTTACCTGGAATTGGACCTTTCACAAGTTGAGCCCTGTATTTCAGGACCAAAGAGGCCTCATGATCGCGTGACCTTGAAAGATATGAAAGCTGACTGGCATGCCTGTCTTGATAACAAGGTTGGGTTCAAGGGATTTGCTATACCAAAAGAGGTACAAGACAAAGAGGTGAAGTTTTCATTCCATGGGCAGCCAGCAGAACTTAAGCATGGTAGTGTTGTCATTGCTGCAATCACAAGCTGCACCAATACATCAAACCCAAGTGTCATGCTTGGGGCTGCTCTTGTTGCAAAAAAGGCCTGCGAACTTGGTCTACGGGTCAAGCCATGGGTAAAAACAAGTCTTGCCCCGGGTTCTGGAGTTGTTACAAAATATCTGCTCCAGAGTGGGTTGCAAAAGTATCTAAATGAACAAGGTTTCAATATTGTTGGATATGGATGCACGACATGCATTGGAAATTCAGGGGATTTAGATGAAAGTGTTGCTTCTGTCATAGCCGACAATGACATAGTAGCAGCTGCTGTGCTTTCTGGAAACAGGAACTTTGAGGGCCGTGTACATCCATTGACAAGGGCTAATTACCTTGCCTCACCTCCTTTAGTGGTTGCTTATGCCCTGGCTGGCACGGTTGACATTGATTTCGATAAGGAGCCAATTGGAATAGGCAAGGATGGTAAGAGCGTCTATTTCAGGGATATCTGGCCATCTTCAGAAGAGATTGCACAGGTTGTGCAATCCAGCGTGTTGCCAGAAATGTTCAGAAGCACTTATGAGTCTATTACTAAAGGCAACCCCATGTGGAATCAGCTGTCGGTTCCAGAAACCAAACTGTACTCTTGGGACCCTAACTCAACCTATATTCATGAGCCCCCATACTTCAAGGGCATGACCATGGATCCTCCTGGTGCTCATGGTGTGAAGGATGCCTACTGCCTGCTAAACTTTGGTGACAGTATTACAACAGATCACATCTCCCCAGCTGGAAGCATCCACAAGGACAGTCCTGCTGCAAAGTACCTTCTTGAACGTGGAGTGGATCGCAAGGACTTCAATTCCTATGGAAGTCGTCGTGGCAATGATGAAGTGATGGCAAGAGGAACTTTTGCCAATATTCGCCTTGTCAACAAGCTATTGAATGGAGAAGTGGGTCCTAAAACAGTTCACATTCCTTCTGGAGAGAAACTCTCTGTGTTCGATGCAGCAACAAAATACAAGTCTGCTGGGCAGGACACCATTGTTTTGGCTGGTGCTGAGTATGGAAGTGGAAGCTCCAGGGATTGGGCTGCCAAGGGCCCAATGTTGTTGGGTGTTAAAGCAGTGATTGCTAAAAGTTTTGAGAGAATCCATCGCAGTAATTTGGTGGGTATGGGTATTATTCCACTTTGTTTCAAAGCTGGTGAGGATGCAGATACACTAGGATTGACTGGTCATGAACGTTATACCATTGACCTCCCTAGCAATATTAGTGAGATAAAGCCTGGCCAAGATGTGACTGTCACCACGGACAGTGGAAAGTCTTTTGTATGCACTGTGCGCTTCGACACTGAGGTGGAATTGGCTTACTTCAACCATGGAGGTATTCTTCCATATGTGATAAGGAACCTGAGCAAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031090 GO:0031667 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048856 GO:0050896 GO:0071704 GO:0071944 GO:0072350 GO:0090351 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

990

Amino Acids

107.97

Weight (kDa)

6.92

Isoelectric Point (pI)

34.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	157 - 660	1.8e-168	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	789 - 917	4.9e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05710
fragaria_vesca	FvH4_6g43190
malus_domestica	MD09G1108800.v1.1 MD17G1096700.v1.1
prunus_persica	Prupe.3G217300_v2.0.a1 Prupe.3G217300_v2.0.a1
pyrus_communis	pycom09g03120 pycom17g09260
rosa_chinensis	RchiOBHm_Chr2g0159291
rosa_laevigata	RLG00000021152
rosa_multiflora	Rmu_sc0004800.1_g000001 Rmu_sc0007806.1_g000010 Rmu_sc0010590.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090620
rosa_rugosa	Rorug02G0475700
rosa_samantha	Rh2AG542100 Rh2BG554700 Rh2CG524800 Rh2DG564400 Rh5AG522200
rosa_wichuraiana	Rw2G044840 Rw5G048460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	640
AccB7I	CCANNNNNTGG	2 cut(s)	174, 2634
AccBSI	CCGCTC	1 cut(s)	140
AccI	GTMKAC	1 cut(s)	1669
AccII	CGCG	4 cut(s)	107, 198, 252, 1425
AciI	CCGC	5 cut(s)	105, 110, 138, 165, 333
AclI	AACGTT	1 cut(s)	2791
AclWI	GGATC	6 cut(s)	272, 368, 389, 2246, 2259, 2400
AcoI	YGGCCR	4 cut(s)	102, 162, 2057, 2836
AcsI	RAATTY	5 cut(s)	474, 513, 725, 1115, 1813
AcuI	CTGAAG	1 cut(s)	2049
AcyI	GRCGYC	1 cut(s)	186
AdeI	CACNNNGTG	1 cut(s)	174
AfaI	GTAC	7 cut(s)	398, 537, 878, 1512, 1914, 2189, 2372
AflII	CTTAAG	1 cut(s)	1559
AflIII	ACRYGT	1 cut(s)	250
AjnI	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1971, 2257, 2627, 2833
AleI	CACNNNNGTG	1 cut(s)	2903
Alw21I	GWGCWC	1 cut(s)	2266
Alw26I	GTCTC	1 cut(s)	1191
AlwI	GGATC	6 cut(s)	272, 368, 389, 2246, 2259, 2400
AlwNI	CAGNNNCTG	1 cut(s)	1877
Ama87I	CYCGRG	1 cut(s)	1705
AoxI	GGCC	8 cut(s)	102, 162, 1412, 1653, 1906, 2057, 2647, 2836
ApaI	GGGCCC	1 cut(s)	2651
ApeKI	GCWGC	9 cut(s)	1546, 1583, 1596, 1634, 1871, 1874, 2363, 2555, 2639
ApoI	RAATTY	5 cut(s)	474, 513, 725, 1115, 1813
ArsI	GACNNNNNNTTYG	4 cut(s)	1979, 2011, 2555, 2587
Asp700I	GAANNNNTTC	1 cut(s)	901
AspLEI	GCGC	5 cut(s)	109, 198, 200, 261, 2892
AspS9I	GGNCC	7 cut(s)	1372, 1403, 1906, 2197, 2504, 2647, 2648
AsuC2I	CCSGG	3 cut(s)	740, 1706, 1707
AsuHPI	GGTGA	7 cut(s)	127, 1157, 1537, 1938, 2315, 2765, 2845
AvaI	CYCGRG	1 cut(s)	1705
AvaII	GGWCC	4 cut(s)	1372, 1403, 2197, 2504
BaeGI	GKGCMC	2 cut(s)	58, 2651
BalI	TGGCCA	2 cut(s)	2059, 2838
BamHI	GGATCC	1 cut(s)	2251
BanI	GGYRCC	1 cut(s)	640
BanII	GRGCYC	3 cut(s)	1392, 2226, 2651
BarI	GAAGNNNNNNTAC	2 cut(s)	2267, 2299
BauI	CACGAG	1 cut(s)	531
Bbv12I	GWGCWC	1 cut(s)	2266
BbvI	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1861, 1883, 2350, 2567, 2626
BccI	CCATC	6 cut(s)	239, 917, 2021, 2068, 2437, 2709
BceAI	ACGGC	2 cut(s)	199, 1893
BciT130I	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1973, 2259, 2629, 2835
BclI	TGATCA	1 cut(s)	661
BcnI	CCSGG	3 cut(s)	740, 1706, 1707
BcoDI	GTCTC	1 cut(s)	1191
BfaI	CTAG	4 cut(s)	51, 722, 2771, 2811
BfmI	CTRYAG	1 cut(s)	352
BfrI	CTTAAG	1 cut(s)	1559
BglI	GCCNNNNNGGC	1 cut(s)	1166
Bme18I	GGWCC	4 cut(s)	1372, 1403, 2197, 2504
BmeT110I	CYCGRG	1 cut(s)	1705
BmgT120I	GGNCC	7 cut(s)	1372, 1403, 1906, 2197, 2504, 2647, 2648
BmrI	ACTGGG	2 cut(s)	493, 1214
BmsI	GCATC	6 cut(s)	583, 1784, 2269, 2352, 2542, 2749
BmuI	ACTGGG	2 cut(s)	493, 1214
BplI	GAGNNNNNCTC	4 cut(s)	2523, 2555, 2525, 2557
BpmI	CTGGAG	5 cut(s)	1029, 1721, 1734, 2550, 2611
Bpu10I	CCTNAGC	1 cut(s)	2960
BpuMI	CCSGG	3 cut(s)	740, 1706, 1707
Bsa29I	ATCGAT	1 cut(s)	384
BsaHI	GRCGYC	1 cut(s)	186
BsaXI	ACNNNNNCTCC	2 cut(s)	262, 292
Bse1I	ACTGG	5 cut(s)	488, 1220, 1261, 1457, 2785
Bse3DI	GCAATG	2 cut(s)	1578, 2437
BseBI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1973, 2259, 2629, 2835
BseCI	ATCGAT	1 cut(s)	384
BseGI	GGATG	7 cut(s)	289, 1799, 1915, 2032, 2284, 2343, 2764
BseMI	GCAATG	2 cut(s)	1578, 2437
BseMII	CTCAG	8 cut(s)	55, 155, 166, 233, 273, 2598, 2892, 2951
BseNI	ACTGG	5 cut(s)	488, 1220, 1261, 1457, 2785
BsePI	GCGCGC	1 cut(s)	196
BseRI	GAGGAG	4 cut(s)	29, 58, 61, 148
BseSI	GKGCMC	2 cut(s)	58, 2651
BseX3I	CGGCCG	1 cut(s)	162
BseXI	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1861, 1883, 2350, 2567, 2626
BseYI	CCCAGC	3 cut(s)	942, 2332, 2576
BsgI	GTGCAG	2 cut(s)	725, 1582
Bsh1236I	CGCG	4 cut(s)	107, 198, 252, 1425
Bsh1285I	CGRYCG	1 cut(s)	165
BshFI	GGCC	8 cut(s)	104, 164, 1414, 1655, 1908, 2059, 2649, 2838
BshNI	GGYRCC	1 cut(s)	640
BshVI	ATCGAT	1 cut(s)	384
BsiEI	CGRYCG	1 cut(s)	165
BsiHKAI	GWGCWC	1 cut(s)	2266
BsiHKCI	CYCGRG	1 cut(s)	1705
BsiSI	CCGG	2 cut(s)	739, 1706
BslFI	GGGAC	1 cut(s)	2210
BsmAI	GTCTC	1 cut(s)	1191
BsmFI	GGGAC	1 cut(s)	2210
BsnI	GGCC	8 cut(s)	104, 164, 1414, 1655, 1908, 2059, 2649, 2838
BsoBI	CYCGRG	1 cut(s)	1705
Bsp120I	GGGCCC	1 cut(s)	2647
Bsp1286I	GDGCHC	5 cut(s)	58, 1392, 2226, 2266, 2651
Bsp1407I	TGTACA	1 cut(s)	1912
Bsp19I	CCATGG	6 cut(s)	273, 1206, 1540, 1682, 2247, 2926
BspACI	CCGC	5 cut(s)	105, 110, 138, 165, 333
BspANI	GGCC	8 cut(s)	104, 164, 1414, 1655, 1908, 2059, 2649, 2838
BspCNI	CTCAG	8 cut(s)	54, 154, 165, 232, 274, 2599, 2893, 2952
BspDI	ATCGAT	1 cut(s)	384
BspFNI	CGCG	4 cut(s)	107, 198, 252, 1425
BspHI	TCATGA	3 cut(s)	1417, 2218, 2785
BspPI	GGATC	6 cut(s)	272, 368, 389, 2246, 2259, 2400
BspT107I	GGYRCC	1 cut(s)	640
BspTI	CTTAAG	1 cut(s)	1559
BsrBI	CCGCTC	1 cut(s)	140
BsrDI	GCAATG	2 cut(s)	1578, 2437
BsrGI	TGTACA	1 cut(s)	1912
BsrI	ACTGG	5 cut(s)	488, 1220, 1261, 1457, 2785
BssHII	GCGCGC	1 cut(s)	196
BssNI	GRCGYC	1 cut(s)	186
BssSI	CACGAG	1 cut(s)	531
BssT1I	CCWWGG	8 cut(s)	273, 636, 1206, 1540, 1682, 2247, 2643, 2926
Bst2BI	CACGAG	1 cut(s)	531
Bst2UI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1973, 2259, 2629, 2835
Bst6I	CTCTTC	3 cut(s)	25, 321, 2064
BstACI	GRCGYC	1 cut(s)	186
BstAFI	CTTAAG	1 cut(s)	1559
BstAPI	GCANNNNNTGC	2 cut(s)	1803, 2678
BstAUI	TGTACA	1 cut(s)	1912
BstDEI	CTNAG	8 cut(s)	41, 141, 152, 219, 282, 2607, 2901, 2960
BstDSI	CCRYGG	8 cut(s)	181, 273, 1206, 1540, 1682, 2247, 2856, 2926
BstF5I	GGATG	7 cut(s)	289, 1799, 1915, 2032, 2284, 2343, 2764
BstFNI	CGCG	4 cut(s)	107, 198, 252, 1425
BstHHI	GCGC	5 cut(s)	109, 198, 200, 261, 2892
BstMAI	GTCTC	1 cut(s)	1191
BstMCI	CGRYCG	1 cut(s)	165
BstNI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1973, 2259, 2629, 2835
BstNSI	RCATGY	2 cut(s)	1460, 1806
BstSFI	CTRYAG	1 cut(s)	352
BstSLI	GKGCMC	2 cut(s)	58, 2651
BstUI	CGCG	4 cut(s)	107, 198, 252, 1425
BstV1I	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1861, 1883, 2350, 2567, 2626
BstX2I	RGATCY	2 cut(s)	373, 2251
BstXI	CCANNNNNNTGG	2 cut(s)	997, 2596
BstYI	RGATCY	2 cut(s)	373, 2251
BstZI	CGGCCG	1 cut(s)	162
Bsu15I	ATCGAT	1 cut(s)	384
BsuRI	GGCC	8 cut(s)	104, 164, 1414, 1655, 1908, 2059, 2649, 2838
BsuTUI	ATCGAT	1 cut(s)	384
BtgI	CCRYGG	8 cut(s)	181, 273, 1206, 1540, 1682, 2247, 2856, 2926
BtgZI	GCGATG	3 cut(s)	217, 1281, 2687
BtsCI	GGATG	7 cut(s)	289, 1799, 1915, 2032, 2284, 2343, 2764
BtsI	GCAGTG	3 cut(s)	619, 708, 2679
BtsIMutI	CAGTG	8 cut(s)	365, 619, 708, 892, 2679, 2869, 2883, 2898
CaiI	CAGNNNCTG	1 cut(s)	1877
CciI	TCATGA	3 cut(s)	1417, 2218, 2785
CfoI	GCGC	5 cut(s)	109, 198, 200, 261, 2892
Cfr13I	GGNCC	7 cut(s)	1372, 1403, 1906, 2197, 2504, 2647, 2648
Cfr9I	CCCGGG	1 cut(s)	1705
ClaI	ATCGAT	1 cut(s)	384
CseI	GACGC	3 cut(s)	175, 241, 2026
CsiI	ACCWGGT	1 cut(s)	802
Csp6I	GTAC	7 cut(s)	397, 536, 877, 1511, 1913, 2188, 2371
CviQI	GTAC	7 cut(s)	397, 536, 877, 1511, 1913, 2188, 2371
DdeI	CTNAG	8 cut(s)	41, 141, 152, 219, 282, 2607, 2901, 2960
DraIII	CACNNNGTG	1 cut(s)	174
EaeI	YGGCCR	4 cut(s)	102, 162, 2057, 2836
EagI	CGGCCG	1 cut(s)	162
Eam1104I	CTCTTC	3 cut(s)	25, 321, 2064
EarI	CTCTTC	3 cut(s)	25, 321, 2064
EclXI	CGGCCG	1 cut(s)	162
Eco130I	CCWWGG	8 cut(s)	273, 636, 1206, 1540, 1682, 2247, 2643, 2926
Eco147I	AGGCCT	2 cut(s)	1414, 1655
Eco24I	GRGCYC	3 cut(s)	1392, 2226, 2651
Eco32I	GATATC	1 cut(s)	2053
Eco47I	GGWCC	4 cut(s)	1372, 1403, 2197, 2504
Eco52I	CGGCCG	1 cut(s)	162
Eco57I	CTGAAG	1 cut(s)	2049
Eco88I	CYCGRG	1 cut(s)	1705
EcoO109I	RGGNCCY	3 cut(s)	2197, 2504, 2647
EcoRI	GAATTC	1 cut(s)	725
EcoRII	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1971, 2257, 2627, 2833
EcoRV	GATATC	1 cut(s)	2053
EcoT14I	CCWWGG	8 cut(s)	273, 636, 1206, 1540, 1682, 2247, 2643, 2926
EcoT22I	ATGCAT	1 cut(s)	1808
EcoT38I	GRGCYC	3 cut(s)	1392, 2226, 2651
ErhI	CCWWGG	8 cut(s)	273, 636, 1206, 1540, 1682, 2247, 2643, 2926
FalI	AAGNNNNNCTT	6 cut(s)	1281, 1313, 1825, 1857, 2440, 2472
FaqI	GGGAC	1 cut(s)	2210
FauNDI	CATATG	1 cut(s)	2944
FbaI	TGATCA	1 cut(s)	661
FblI	GTMKAC	1 cut(s)	1669
FokI	GGATG	7 cut(s)	276, 1806, 1902, 2039, 2291, 2330, 2771
FriOI	GRGCYC	3 cut(s)	1392, 2226, 2651
FspBI	CTAG	4 cut(s)	51, 722, 2771, 2811
GlaI	GCGC	5 cut(s)	108, 197, 199, 260, 2891
GsaI	CCCAGC	3 cut(s)	946, 2336, 2580
GsuI	CTGGAG	5 cut(s)	1029, 1721, 1734, 2550, 2611
HaeIII	GGCC	8 cut(s)	104, 164, 1414, 1655, 1908, 2059, 2649, 2838
HapII	CCGG	2 cut(s)	739, 1706
HgaI	GACGC	3 cut(s)	175, 241, 2026
HhaI	GCGC	5 cut(s)	109, 198, 200, 261, 2892
Hin1I	GRCGYC	1 cut(s)	186
Hin6I	GCGC	5 cut(s)	107, 196, 198, 259, 2890
HinP1I	GCGC	5 cut(s)	107, 196, 198, 259, 2890
HincII	GTYRAC	4 cut(s)	574, 1321, 1987, 2479
HindII	GTYRAC	4 cut(s)	574, 1321, 1987, 2479
HinfI	GANTC	9 cut(s)	286, 408, 419, 617, 902, 1337, 2129, 2158, 2697
HpaII	CCGG	2 cut(s)	739, 1706
HphI	GGTGA	7 cut(s)	127, 1157, 1537, 1938, 2315, 2765, 2845
Hpy99I	CGWCG	2 cut(s)	127, 2427
HpyAV	CCTTC	7 cut(s)	106, 129, 451, 1283, 2269, 2384, 2535
HpyCH4IV	ACGT	2 cut(s)	2383, 2791
HpyF3I	CTNAG	8 cut(s)	41, 141, 152, 219, 282, 2607, 2901, 2960
HpySE526I	ACGT	2 cut(s)	2383, 2791
Hsp92I	GRCGYC	1 cut(s)	186
HspAI	GCGC	5 cut(s)	107, 196, 198, 259, 2890
KflI	GGGWCCC	1 cut(s)	2197
Ksp22I	TGATCA	1 cut(s)	661
Lsp1109I	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1861, 1883, 2350, 2567, 2626
LweI	GCATC	6 cut(s)	583, 1784, 2269, 2352, 2542, 2749
MabI	ACCWGGT	1 cut(s)	802
MaeI	CTAG	4 cut(s)	51, 722, 2771, 2811
MaeII	ACGT	2 cut(s)	2383, 2791
MbiI	CCGCTC	1 cut(s)	140
MboII	GAAGA	9 cut(s)	12, 55, 308, 473, 1207, 1289, 2055, 2081, 2930
MfeI	CAATTG	1 cut(s)	2010
MflI	RGATCY	2 cut(s)	373, 2251
MhlI	GDGCHC	5 cut(s)	58, 1392, 2226, 2266, 2651
MlsI	TGGCCA	2 cut(s)	2059, 2838
MluI	ACGCGT	1 cut(s)	250
MluNI	TGGCCA	2 cut(s)	2059, 2838
MlyI	GAGTC	2 cut(s)	295, 2138
MmeI	TCCRAC	2 cut(s)	874, 1765
Mox20I	TGGCCA	2 cut(s)	2059, 2838
Mph1103I	ATGCAT	1 cut(s)	1808
MroXI	GAANNNNTTC	1 cut(s)	901
MscI	TGGCCA	2 cut(s)	2059, 2838
MseI	TTAA	3 cut(s)	759, 1560, 2667
MslI	CAYNNNNRTG	5 cut(s)	995, 1085, 1569, 2246, 2903
Msp20I	TGGCCA	2 cut(s)	2059, 2838
MspA1I	CMGCKG	4 cut(s)	566, 1874, 2164, 2336
MspCI	CTTAAG	1 cut(s)	1559
MspI	CCGG	2 cut(s)	739, 1706
MunI	CAATTG	1 cut(s)	2010
MvaI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1973, 2259, 2629, 2835
MvnI	CGCG	4 cut(s)	107, 198, 252, 1425
NciI	CCSGG	3 cut(s)	740, 1706, 1707
NcoI	CCATGG	6 cut(s)	273, 1206, 1540, 1682, 2247, 2926
NdeI	CATATG	1 cut(s)	2944
NmeAIII	GCCGAG	2 cut(s)	11, 140
NmuCI	GTSAC	6 cut(s)	437, 1078, 1426, 2303, 2845, 2851
NsiI	ATGCAT	1 cut(s)	1808
NspI	RCATGY	2 cut(s)	1460, 1806
OliI	CACNNNNGTG	1 cut(s)	2903
PaeI	GCATGC	1 cut(s)	1460
PagI	TCATGA	3 cut(s)	1417, 2218, 2785
PauI	GCGCGC	1 cut(s)	196
PceI	AGGCCT	2 cut(s)	1414, 1655
PdmI	GAANNNNTTC	1 cut(s)	901
PfeI	GAWTC	7 cut(s)	408, 419, 617, 902, 1337, 2158, 2697
PflFI	GACNNNGTC	1 cut(s)	1067
PflMI	CCANNNNNTGG	2 cut(s)	174, 2634
PleI	GAGTC	2 cut(s)	294, 2137
PpsI	GAGTC	2 cut(s)	294, 2137
PpuMI	RGGWCCY	2 cut(s)	2197, 2504
Psp1406I	AACGTT	1 cut(s)	2791
Psp5II	RGGWCCY	2 cut(s)	2197, 2504
Psp6I	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1971, 2257, 2627, 2833
PspFI	CCCAGC	3 cut(s)	942, 2332, 2576
PspGI	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1971, 2257, 2627, 2833
PspOMI	GGGCCC	1 cut(s)	2647
PspPI	GGNCC	7 cut(s)	1372, 1403, 1906, 2197, 2504, 2647, 2648
PspPPI	RGGWCCY	2 cut(s)	2197, 2504
PsrI	GAACNNNNNNTAC	4 cut(s)	332, 364, 1653, 1685
PstNI	CAGNNNCTG	1 cut(s)	1877
PsuI	RGATCY	2 cut(s)	373, 2251
PsyI	GACNNNGTC	1 cut(s)	1067
PteI	GCGCGC	1 cut(s)	196
PvuII	CAGCTG	4 cut(s)	566, 1874, 2164, 2336
RsaI	GTAC	7 cut(s)	398, 537, 878, 1512, 1914, 2189, 2372
RsaNI	GTAC	7 cut(s)	397, 536, 877, 1511, 1913, 2188, 2371
RseI	CAYNNNNRTG	5 cut(s)	995, 1085, 1569, 2246, 2903
SaqAI	TTAA	3 cut(s)	759, 1560, 2667
Sau96I	GGNCC	7 cut(s)	1372, 1403, 1906, 2197, 2504, 2647, 2648
SchI	GAGTC	2 cut(s)	295, 2138
SduI	GDGCHC	5 cut(s)	58, 1392, 2226, 2266, 2651
SexAI	ACCWGGT	1 cut(s)	802
SfaNI	GCATC	6 cut(s)	583, 1784, 2269, 2352, 2542, 2749
SfcI	CTRYAG	1 cut(s)	352
SinI	GGWCC	4 cut(s)	1372, 1403, 2197, 2504
SmaI	CCCGGG	1 cut(s)	1707
SmiMI	CAYNNNNRTG	5 cut(s)	995, 1085, 1569, 2246, 2903
SmlI	CTYRAG	1 cut(s)	1559
SmoI	CTYRAG	1 cut(s)	1559
SphI	GCATGC	1 cut(s)	1460
SseBI	AGGCCT	2 cut(s)	1414, 1655
SsiI	CCGC	5 cut(s)	105, 110, 138, 165, 333
SspI	AATATT	3 cut(s)	1780, 2467, 2818
SspMI	CTAG	4 cut(s)	51, 722, 2771, 2811
StuI	AGGCCT	2 cut(s)	1414, 1655
StyI	CCWWGG	8 cut(s)	273, 636, 1206, 1540, 1682, 2247, 2643, 2926
TaiI	ACGT	2 cut(s)	2386, 2794
TaqI	TCGA	6 cut(s)	246, 384, 678, 1998, 2550, 2895
TatI	WGTACW	3 cut(s)	535, 1912, 2187
TauI	GCSGC	4 cut(s)	107, 112, 167, 336
TfiI	GAWTC	7 cut(s)	408, 419, 617, 902, 1337, 2158, 2697
Tru1I	TTAA	3 cut(s)	759, 1560, 2667
Tru9I	TTAA	3 cut(s)	759, 1560, 2667
TscAI	CASTG	8 cut(s)	372, 619, 708, 892, 2679, 2869, 2890, 2905
TseFI	GTSAC	6 cut(s)	437, 1078, 1426, 2303, 2845, 2851
TseI	GCWGC	9 cut(s)	1546, 1583, 1596, 1634, 1871, 1874, 2363, 2555, 2639
Tsp45I	GTSAC	6 cut(s)	437, 1078, 1426, 2303, 2845, 2851
TspGWI	ACGGA	2 cut(s)	658, 2873
TspMI	CCCGGG	1 cut(s)	1705
TspRI	CASTG	8 cut(s)	372, 619, 708, 892, 2679, 2869, 2890, 2905
Tth111I	GACNNNGTC	1 cut(s)	1067
Van91I	CCANNNNNTGG	2 cut(s)	174, 2634
Vha464I	CTTAAG	1 cut(s)	1559
VpaK11BI	GGWCC	4 cut(s)	1372, 1403, 2197, 2504
XapI	RAATTY	5 cut(s)	474, 513, 725, 1115, 1813
XceI	RCATGY	2 cut(s)	1460, 1806
XcmI	CCANNNNNNNNNTGG	1 cut(s)	97
XmaI	CCCGGG	1 cut(s)	1705
XmiI	GTMKAC	1 cut(s)	1669
XmnI	GAANNNNTTC	1 cut(s)	901
XspI	CTAG	4 cut(s)	51, 722, 2771, 2811
Zsp2I	ATGCAT	1 cut(s)	1808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.