Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0009004

Physical Location & Seq

Genomic Coordinates Forward (+)

79384133 .. 79391433

Gene Structure 7301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG564400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGCGTGATGCTATGAACAATCTTGGCAGTGATTCTAACAAGATCAATCCCTTGGTTCCTGTTGATCTTGTTGTTGATCACTCCGTTCAAGTTGATGTTGCAAGATCAGAAAATGCAGTGCAGGCAAATATGGAGCTAGAATTCCAGAGAAACCGGGAGAGATTTGCTTTTCTTAAATGGGGTTCTACTGCTTTCCAAAATATGCTTGTTGTTCCACCTGGTTCTGGCATTGTTCATCAGGTCAATCTTGAATATCTTGGACGGGTTGTTTTCAACACTAATGGCTTGCTGTACCCCGACAGTGTGGTTGGAACTGATTCCCATACAACCATGATTGATGGTTTGGGAGTAGCTGGCTGGGGAGTTGGGGGTATTGAAGCAGAAGCTACTATGCTTGGTCAGCCCATGAGCATGGTGTTGCCTGGAGTTGTTGGGTTCAAGTTATCTGGAAAGTTGAACAATGGTGTTACTGCTACTGACTTGGTCTTGACTGTCACACAAATGCTGAGGAAGCATGGAGTTGTGGGAAAATTTGTTGAGTTTTATGGGGGTGGTATGGGTGAACTGTCGTTAGCCGACAGGGCTACTATTGCCAATATGTCTCCTGAATATGGTGCAACCATGGGGTTCTTCCCAGTAGATCATGTTACTTTACAATATCTCAAATTGACTGGTAGAAGCGATGAAACTGTGGCAATGATTGAAGGTTATCTTCGGGCAAATAAATTGTTTGTTGACTATAATGAGCCTGAATCAGAAAGAGTTTACTCATCTTACCTGGAATTGGACCTTTCACAAGTTGAGCCCTGTATTTCAGGACCAAAGAGACCTCATGATCGCGTGACCTTGAAAGATATGAAAGCTGACTGGCATGCCTGTCTTGATAACAAGGTTGGGTTCAAGGGATTTGCTATACCAAAAGAGGTACAAGACAAAGAGGTGAAGTTTTCATTCCATGGGCAGCCAGCAGAACTTAAGCATGGTAGTGTTGTCATTGCTGCAATCACAAGCTGCACCAATACATCAAACCCAAGTGTCATGCTTGGGGCTGCTCTTGTTGCAAAAAAGGCCTGCGAACTTGGTCTACGGGTCAAGCCATGGGTAAAAACAAGTCTTGCCCCGGGTTCTGGAGTTGTTACAAAATATCTGCTCCAGAGTGGGTTGCAAAAGTATCTAAATGAACAGGGTTTCAATATTGTTGGATATGGATGCACGACATGCATTGGAAATTCAGGGGATTTAGATGAAACTGTTGCTTCTGTCATAGCCGACAATGACGTAGTAGCAGCTGCTGTGCTTTCTGGAAACAGGAACTTTGAGGGCCGTGTACATCCATTGACAAGGGCTAATTACCTTGCCTCACCTCCTTTAGTGGTTGCTTATGCCCTGGCTGGCACGGTTGACATTGATTTCGATAAGGAGCCAATTGGAACAGGCAAGGATGGTAAGAGCGTCTATTTTAGGGATATCTGGCCATCTTCAGAAGAGATTGCACAGGTTGTGCAATCCAGCGTGTTGCCAGAAATGTTCAGAAGCACTTATGAGTCTATTACTAAAGGCAACCCCATGTGGAATCAGCTGTCGGTTCCAGAAACCAAACTGTACTCTTGGGACCCTAACTCAACCTATATTCATGAGCCCCCATACTTCAAGGGCATGACCATGGATCCTCCTGGTGCTCATGGTGTGAAGGATGCCTACTGCCTGTTAAACTTTGGTGACAGTATTACAACAGATCACATCTCCCCAGCTGGAAGCATCCACAAGGATAGTCCTGCTGCAAAGTACCTTCTTGAACGTGGAGTGGATCGCAAGGACTTCAATTCCTATGGAAGTCGTCGTGGCAATGATGAAGTGATGGCAAGAGGAACTTTTGCCAATATTCGCCTTGTCAACAAGCTATTGAATGGAGAAGTGGGTCCTAAAACAGTTCACATTCCTTCCGGAGAGAAACTCTCTGTGTTCGATGCTGCAACGAAATACAAGTCTGCTGGGCAGGACACCATTGTTTTGGCTGGTGCTGAGTATGGAAGTGGAAGCTCCAGGGATTGGGCTGCCAAGGGCCCAATGTTGTTGGGTGTTAAAGCAGTGATTGCTAAAAGTTTTGAGAGAATCCATCGCAGTAATTTGGTGGGTATGGGTATTGTTCCACTTTGTTTCAAAGCTGGTGAGGATGCAGATACACTAGGATTGACTGGTCATGAACGTTATACCATTGACCTCCCTAGCAATATTAGTGAGATAAAGCCTGGCCAAGATGTGACTGTCACCACGGACAGTGGAAAGTCTTTTGTATGCACTGTGCGCTTTGACACTGAGGTGGAATTGGCTTACTTCAACCATGGAGGTATTCTTCCATATGTGATAAGGAACCTGAGCAAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031090 GO:0031667 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048856 GO:0050896 GO:0071704 GO:0071944 GO:0072350 GO:0090351 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

795

Amino Acids

86.23

Weight (kDa)

5.89

Isoelectric Point (pI)

30.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	1 - 465	4.3e-157	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	594 - 722	7.1e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05710
fragaria_vesca	FvH4_6g43190
malus_domestica	MD09G1108800.v1.1 MD17G1096700.v1.1
prunus_persica	Prupe.3G217300_v2.0.a1 Prupe.3G217300_v2.0.a1
pyrus_communis	pycom09g03120 pycom17g09260
rosa_chinensis	RchiOBHm_Chr2g0159291
rosa_laevigata	RLG00000021152
rosa_multiflora	Rmu_sc0004800.1_g000001 Rmu_sc0007806.1_g000010 Rmu_sc0010590.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090620
rosa_rugosa	Rorug02G0475700
rosa_samantha	Rh2AG542100 Rh2BG554700 Rh2CG524800 Rh2DG564400 Rh5AG522200
rosa_wichuraiana	Rw2G044840 Rw5G048460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2049
AccI	GTMKAC	1 cut(s)	1084
AccII	CGCG	1 cut(s)	840
AccIII	TCCGGA	1 cut(s)	1943
AclI	AACGTT	1 cut(s)	2206
AclWI	GGATC	3 cut(s)	1661, 1674, 1815
AcoI	YGGCCR	2 cut(s)	1472, 2251
AcsI	RAATTY	3 cut(s)	140, 530, 1228
AcuI	CTGAAG	1 cut(s)	1464
AfaI	GTAC	5 cut(s)	293, 927, 1329, 1604, 1787
AfiI	CCNNNNNNNGG	5 cut(s)	224, 611, 784, 1127, 2049
AflII	CTTAAG	1 cut(s)	974
AjnI	CCWGG	7 cut(s)	217, 421, 777, 1386, 1672, 2042, 2248
AleI	CACNNNNGTG	1 cut(s)	2318
Alw21I	GWGCWC	1 cut(s)	1681
Alw26I	GTCTC	2 cut(s)	606, 820
AlwI	GGATC	3 cut(s)	1661, 1674, 1815
AlwNI	CAGNNNCTG	1 cut(s)	1292
Ama87I	CYCGRG	1 cut(s)	1120
Aor13HI	TCCGGA	1 cut(s)	1943
AoxI	GGCC	5 cut(s)	1068, 1321, 1472, 2062, 2251
ApaI	GGGCCC	1 cut(s)	2066
ApeKI	GCWGC	9 cut(s)	961, 998, 1011, 1049, 1286, 1289, 1778, 1970, 2054
ApoI	RAATTY	3 cut(s)	140, 530, 1228
ArsI	GACNNNNNNTTYG	4 cut(s)	1394, 1426, 1970, 2002
Asp700I	GAANNNNTTC	1 cut(s)	316
AspLEI	GCGC	1 cut(s)	2307
AspS9I	GGNCC	7 cut(s)	787, 818, 1321, 1612, 1919, 2062, 2063
AsuC2I	CCSGG	3 cut(s)	155, 1121, 1122
AsuHPI	GGTGA	6 cut(s)	572, 952, 1353, 1730, 2180, 2260
AvaI	CYCGRG	1 cut(s)	1120
AvaII	GGWCC	4 cut(s)	787, 818, 1612, 1919
BaeGI	GKGCMC	1 cut(s)	2066
BalI	TGGCCA	2 cut(s)	1474, 2253
BamHI	GGATCC	1 cut(s)	1666
BanII	GRGCYC	3 cut(s)	807, 1641, 2066
BarI	GAAGNNNNNNTAC	2 cut(s)	1682, 1714
Bbv12I	GWGCWC	1 cut(s)	1681
BbvCI	CCTCAGC	1 cut(s)	506
BbvI	GCAGC	9 cut(s)	973, 985, 998, 1036, 1276, 1298, 1765, 1957, 2041
BccI	CCATC	5 cut(s)	332, 1436, 1483, 1852, 2124
BceAI	ACGGC	1 cut(s)	1308
BciT130I	CCWGG	7 cut(s)	219, 423, 779, 1388, 1674, 2044, 2250
BclI	TGATCA	1 cut(s)	76
BcnI	CCSGG	3 cut(s)	155, 1121, 1122
BcoDI	GTCTC	2 cut(s)	606, 820
BfaI	CTAG	3 cut(s)	137, 2186, 2226
BfrI	CTTAAG	1 cut(s)	974
BglI	GCCNNNNNGGC	1 cut(s)	581
BisI	GCNGC	9 cut(s)	962, 999, 1012, 1050, 1287, 1290, 1779, 1971, 2055
BlsI	GCNGC	9 cut(s)	963, 1000, 1013, 1051, 1288, 1291, 1780, 1972, 2056
Bme18I	GGWCC	4 cut(s)	787, 818, 1612, 1919
BmeT110I	CYCGRG	1 cut(s)	1120
BmgT120I	GGNCC	7 cut(s)	787, 818, 1321, 1612, 1919, 2062, 2063
BmiI	GGNNCC	9 cut(s)	57, 1422, 1587, 1613, 1614, 1668, 1920, 2064, 2372
BmrI	ACTGGG	1 cut(s)	629
BmsI	GCATC	5 cut(s)	1199, 1684, 1767, 1957, 2164
BmuI	ACTGGG	1 cut(s)	629
BplI	GAGNNNNNCTC	4 cut(s)	1938, 1970, 1940, 1972
BpmI	CTGGAG	4 cut(s)	444, 1136, 1149, 2026
Bpu10I	CCTNAGC	2 cut(s)	506, 2375
BpuMI	CCSGG	3 cut(s)	155, 1121, 1122
BsaI	GGTCTC	1 cut(s)	820
BsaWI	WCCGGW	1 cut(s)	1943
Bsc4I	CCNNNNNNNGG	5 cut(s)	224, 611, 784, 1127, 2049
Bse1I	ACTGG	4 cut(s)	635, 676, 872, 2200
Bse3DI	GCAATG	3 cut(s)	702, 993, 1852
BseAI	TCCGGA	1 cut(s)	1943
BseBI	CCWGG	7 cut(s)	219, 423, 779, 1388, 1674, 2044, 2250
BseGI	GGATG	6 cut(s)	1214, 1330, 1447, 1699, 1758, 2179
BseLI	CCNNNNNNNGG	5 cut(s)	224, 611, 784, 1127, 2049
BseMI	GCAATG	3 cut(s)	702, 993, 1852
BseMII	CTCAG	4 cut(s)	497, 2013, 2307, 2366
BseNI	ACTGG	4 cut(s)	635, 676, 872, 2200
BseSI	GKGCMC	1 cut(s)	2066
BseXI	GCAGC	9 cut(s)	973, 985, 998, 1036, 1276, 1298, 1765, 1957, 2041
BseYI	CCCAGC	3 cut(s)	357, 1747, 1991
BsgI	GTGCAG	2 cut(s)	140, 997
Bsh1236I	CGCG	1 cut(s)	840
BshFI	GGCC	5 cut(s)	1070, 1323, 1474, 2064, 2253
BsiHKAI	GWGCWC	1 cut(s)	1681
BsiHKCI	CYCGRG	1 cut(s)	1120
BsiSI	CCGG	3 cut(s)	154, 1121, 1944
BslFI	GGGAC	1 cut(s)	1625
BslI	CCNNNNNNNGG	5 cut(s)	224, 611, 784, 1127, 2049
BsmAI	GTCTC	2 cut(s)	606, 820
BsmFI	GGGAC	1 cut(s)	1625
BsnI	GGCC	5 cut(s)	1070, 1323, 1474, 2064, 2253
Bso31I	GGTCTC	1 cut(s)	820
BsoBI	CYCGRG	1 cut(s)	1120
Bsp120I	GGGCCC	1 cut(s)	2062
Bsp1286I	GDGCHC	4 cut(s)	807, 1641, 1681, 2066
Bsp13I	TCCGGA	1 cut(s)	1943
Bsp1407I	TGTACA	1 cut(s)	1327
Bsp143I	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
Bsp19I	CCATGG	5 cut(s)	621, 955, 1097, 1662, 2341
BspANI	GGCC	5 cut(s)	1070, 1323, 1474, 2064, 2253
BspCNI	CTCAG	4 cut(s)	498, 2014, 2308, 2367
BspEI	TCCGGA	1 cut(s)	1943
BspFNI	CGCG	1 cut(s)	840
BspHI	TCATGA	3 cut(s)	832, 1633, 2200
BspLI	GGNNCC	9 cut(s)	57, 1422, 1587, 1613, 1614, 1668, 1920, 2064, 2372
BspPI	GGATC	3 cut(s)	1661, 1674, 1815
BspTI	CTTAAG	1 cut(s)	974
BspTNI	GGTCTC	1 cut(s)	820
BsrDI	GCAATG	3 cut(s)	702, 993, 1852
BsrGI	TGTACA	1 cut(s)	1327
BsrI	ACTGG	4 cut(s)	635, 676, 872, 2200
BssMI	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
BssT1I	CCWWGG	7 cut(s)	51, 621, 955, 1097, 1662, 2058, 2341
Bst2UI	CCWGG	7 cut(s)	219, 423, 779, 1388, 1674, 2044, 2250
Bst6I	CTCTTC	1 cut(s)	1479
BstAFI	CTTAAG	1 cut(s)	974
BstAPI	GCANNNNNTGC	2 cut(s)	1218, 2093
BstAUI	TGTACA	1 cut(s)	1327
BstC8I	GCNNGC	8 cut(s)	123, 287, 355, 873, 966, 1072, 1393, 2381
BstDEI	CTNAG	4 cut(s)	506, 2022, 2316, 2375
BstDSI	CCRYGG	6 cut(s)	621, 955, 1097, 1662, 2271, 2341
BstF5I	GGATG	6 cut(s)	1214, 1330, 1447, 1699, 1758, 2179
BstFNI	CGCG	1 cut(s)	840
BstHHI	GCGC	1 cut(s)	2307
BstKTI	GATC	9 cut(s)	45, 67, 79, 107, 643, 838, 1669, 1738, 1810
BstMAI	GTCTC	2 cut(s)	606, 820
BstMBI	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
BstNI	CCWGG	7 cut(s)	219, 423, 779, 1388, 1674, 2044, 2250
BstNSI	RCATGY	2 cut(s)	875, 1221
BstSLI	GKGCMC	1 cut(s)	2066
BstUI	CGCG	1 cut(s)	840
BstV1I	GCAGC	9 cut(s)	973, 985, 998, 1036, 1276, 1298, 1765, 1957, 2041
BstX2I	RGATCY	1 cut(s)	1666
BstXI	CCANNNNNNTGG	2 cut(s)	412, 2011
BstYI	RGATCY	1 cut(s)	1666
BsuRI	GGCC	5 cut(s)	1070, 1323, 1474, 2064, 2253
BtgI	CCRYGG	6 cut(s)	621, 955, 1097, 1662, 2271, 2341
BtgZI	GCGATG	2 cut(s)	696, 2102
BtsCI	GGATG	6 cut(s)	1214, 1330, 1447, 1699, 1758, 2179
BtsI	GCAGTG	3 cut(s)	34, 123, 2094
BtsIMutI	CAGTG	7 cut(s)	34, 123, 307, 2094, 2284, 2298, 2313
Cac8I	GCNNGC	8 cut(s)	123, 287, 355, 873, 966, 1072, 1393, 2381
CaiI	CAGNNNCTG	1 cut(s)	1292
CciI	TCATGA	3 cut(s)	832, 1633, 2200
CfoI	GCGC	1 cut(s)	2307
Cfr13I	GGNCC	7 cut(s)	787, 818, 1321, 1612, 1919, 2062, 2063
Cfr9I	CCCGGG	1 cut(s)	1120
CseI	GACGC	1 cut(s)	1441
CsiI	ACCWGGT	1 cut(s)	217
Csp6I	GTAC	5 cut(s)	292, 926, 1328, 1603, 1786
CviQI	GTAC	5 cut(s)	292, 926, 1328, 1603, 1786
DdeI	CTNAG	4 cut(s)	506, 2022, 2316, 2375
DpnI	GATC	9 cut(s)	44, 66, 78, 106, 642, 837, 1668, 1737, 1809
DpnII	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
EaeI	YGGCCR	2 cut(s)	1472, 2251
Eam1104I	CTCTTC	1 cut(s)	1479
EarI	CTCTTC	1 cut(s)	1479
Eco130I	CCWWGG	7 cut(s)	51, 621, 955, 1097, 1662, 2058, 2341
Eco147I	AGGCCT	1 cut(s)	1070
Eco24I	GRGCYC	3 cut(s)	807, 1641, 2066
Eco31I	GGTCTC	1 cut(s)	820
Eco32I	GATATC	1 cut(s)	1468
Eco47I	GGWCC	4 cut(s)	787, 818, 1612, 1919
Eco57I	CTGAAG	1 cut(s)	1464
Eco88I	CYCGRG	1 cut(s)	1120
EcoO109I	RGGNCCY	3 cut(s)	1612, 1919, 2062
EcoRI	GAATTC	1 cut(s)	140
EcoRII	CCWGG	7 cut(s)	217, 421, 777, 1386, 1672, 2042, 2248
EcoRV	GATATC	1 cut(s)	1468
EcoT14I	CCWWGG	7 cut(s)	51, 621, 955, 1097, 1662, 2058, 2341
EcoT22I	ATGCAT	1 cut(s)	1223
EcoT38I	GRGCYC	3 cut(s)	807, 1641, 2066
ErhI	CCWWGG	7 cut(s)	51, 621, 955, 1097, 1662, 2058, 2341
FalI	AAGNNNNNCTT	4 cut(s)	696, 728, 1855, 1887
FaqI	GGGAC	1 cut(s)	1625
FauNDI	CATATG	1 cut(s)	2359
FbaI	TGATCA	1 cut(s)	76
FblI	GTMKAC	1 cut(s)	1084
Fnu4HI	GCNGC	9 cut(s)	962, 999, 1012, 1050, 1287, 1290, 1779, 1971, 2055
FokI	GGATG	6 cut(s)	1221, 1317, 1454, 1706, 1745, 2186
FriOI	GRGCYC	3 cut(s)	807, 1641, 2066
Fsp4HI	GCNGC	9 cut(s)	962, 999, 1012, 1050, 1287, 1290, 1779, 1971, 2055
FspBI	CTAG	3 cut(s)	137, 2186, 2226
GlaI	GCGC	1 cut(s)	2306
GluI	GCNGC	9 cut(s)	962, 999, 1012, 1050, 1287, 1290, 1779, 1971, 2055
GsaI	CCCAGC	3 cut(s)	361, 1751, 1995
GsuI	CTGGAG	4 cut(s)	444, 1136, 1149, 2026
HaeIII	GGCC	5 cut(s)	1070, 1323, 1474, 2064, 2253
HapII	CCGG	3 cut(s)	154, 1121, 1944
HgaI	GACGC	1 cut(s)	1441
HhaI	GCGC	1 cut(s)	2307
Hin6I	GCGC	1 cut(s)	2305
HinP1I	GCGC	1 cut(s)	2305
HincII	GTYRAC	3 cut(s)	736, 1402, 1894
HindII	GTYRAC	3 cut(s)	736, 1402, 1894
HinfI	GANTC	6 cut(s)	32, 317, 752, 1544, 1573, 2112
HpaII	CCGG	3 cut(s)	154, 1121, 1944
HphI	GGTGA	6 cut(s)	572, 952, 1353, 1730, 2180, 2260
Hpy166II	GTNNAC	8 cut(s)	563, 736, 766, 1085, 1328, 1402, 1894, 1933
Hpy188I	TCNGA	4 cut(s)	109, 757, 1483, 1532
Hpy8I	GTNNAC	8 cut(s)	563, 736, 766, 1085, 1328, 1402, 1894, 1933
Hpy99I	CGWCG	1 cut(s)	1842
HpyAV	CCTTC	4 cut(s)	698, 1684, 1799, 1950
HpyCH4IV	ACGT	3 cut(s)	1278, 1798, 2206
HpyF3I	CTNAG	4 cut(s)	506, 2022, 2316, 2375
HpySE526I	ACGT	3 cut(s)	1278, 1798, 2206
HspAI	GCGC	1 cut(s)	2305
KflI	GGGWCCC	1 cut(s)	1612
Kpn2I	TCCGGA	1 cut(s)	1943
Ksp22I	TGATCA	1 cut(s)	76
Kzo9I	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
LmnI	GCTCC	4 cut(s)	133, 1155, 1420, 2045
Lsp1109I	GCAGC	9 cut(s)	973, 985, 998, 1036, 1276, 1298, 1765, 1957, 2041
LweI	GCATC	5 cut(s)	1199, 1684, 1767, 1957, 2164
MabI	ACCWGGT	1 cut(s)	217
MaeI	CTAG	3 cut(s)	137, 2186, 2226
MaeII	ACGT	3 cut(s)	1278, 1798, 2206
MaeIII	GTNAC	8 cut(s)	466, 493, 646, 841, 1135, 1718, 2260, 2266
MalI	GATC	9 cut(s)	44, 66, 78, 106, 642, 837, 1668, 1737, 1809
MboI	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
MboII	GAAGA	5 cut(s)	622, 704, 1470, 1496, 2345
MfeI	CAATTG	1 cut(s)	1425
MflI	RGATCY	1 cut(s)	1666
MhlI	GDGCHC	4 cut(s)	807, 1641, 1681, 2066
MlsI	TGGCCA	2 cut(s)	1474, 2253
MluNI	TGGCCA	2 cut(s)	1474, 2253
MlyI	GAGTC	1 cut(s)	1553
MmeI	TCCRAC	2 cut(s)	289, 1180
Mox20I	TGGCCA	2 cut(s)	1474, 2253
Mph1103I	ATGCAT	1 cut(s)	1223
MroI	TCCGGA	1 cut(s)	1943
MroXI	GAANNNNTTC	1 cut(s)	316
MscI	TGGCCA	2 cut(s)	1474, 2253
MseI	TTAA	4 cut(s)	174, 975, 1709, 2082
MslI	CAYNNNNRTG	5 cut(s)	410, 500, 984, 1661, 2318
Msp20I	TGGCCA	2 cut(s)	1474, 2253
MspA1I	CMGCKG	3 cut(s)	1289, 1579, 1751
MspCI	CTTAAG	1 cut(s)	974
MspI	CCGG	3 cut(s)	154, 1121, 1944
MunI	CAATTG	1 cut(s)	1425
MvaI	CCWGG	7 cut(s)	219, 423, 779, 1388, 1674, 2044, 2250
MvnI	CGCG	1 cut(s)	840
NciI	CCSGG	3 cut(s)	155, 1121, 1122
NcoI	CCATGG	5 cut(s)	621, 955, 1097, 1662, 2341
NdeI	CATATG	1 cut(s)	2359
NdeII	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
NlaIV	GGNNCC	9 cut(s)	57, 1422, 1587, 1613, 1614, 1668, 1920, 2064, 2372
NmuCI	GTSAC	5 cut(s)	493, 841, 1718, 2260, 2266
NsiI	ATGCAT	1 cut(s)	1223
NspI	RCATGY	2 cut(s)	875, 1221
OliI	CACNNNNGTG	1 cut(s)	2318
PaeI	GCATGC	1 cut(s)	875
PagI	TCATGA	3 cut(s)	832, 1633, 2200
PceI	AGGCCT	1 cut(s)	1070
PdmI	GAANNNNTTC	1 cut(s)	316
PfeI	GAWTC	5 cut(s)	32, 317, 752, 1573, 2112
PflFI	GACNNNGTC	1 cut(s)	482
PflMI	CCANNNNNTGG	1 cut(s)	2049
PkrI	GCNGC	9 cut(s)	963, 1000, 1013, 1051, 1288, 1291, 1780, 1972, 2056
PleI	GAGTC	1 cut(s)	1552
PpsI	GAGTC	1 cut(s)	1552
PpuMI	RGGWCCY	2 cut(s)	1612, 1919
Psp1406I	AACGTT	1 cut(s)	2206
Psp5II	RGGWCCY	2 cut(s)	1612, 1919
Psp6I	CCWGG	7 cut(s)	217, 421, 777, 1386, 1672, 2042, 2248
PspFI	CCCAGC	3 cut(s)	357, 1747, 1991
PspGI	CCWGG	7 cut(s)	217, 421, 777, 1386, 1672, 2042, 2248
PspN4I	GGNNCC	9 cut(s)	57, 1422, 1587, 1613, 1614, 1668, 1920, 2064, 2372
PspOMI	GGGCCC	1 cut(s)	2062
PspPI	GGNCC	7 cut(s)	787, 818, 1321, 1612, 1919, 2062, 2063
PspPPI	RGGWCCY	2 cut(s)	1612, 1919
PsrI	GAACNNNNNNTAC	2 cut(s)	1068, 1100
PstNI	CAGNNNCTG	1 cut(s)	1292
PsuI	RGATCY	1 cut(s)	1666
PsyI	GACNNNGTC	1 cut(s)	482
PvuII	CAGCTG	3 cut(s)	1289, 1579, 1751
RsaI	GTAC	5 cut(s)	293, 927, 1329, 1604, 1787
RsaNI	GTAC	5 cut(s)	292, 926, 1328, 1603, 1786
RseI	CAYNNNNRTG	5 cut(s)	410, 500, 984, 1661, 2318
SaqAI	TTAA	4 cut(s)	174, 975, 1709, 2082
SatI	GCNGC	9 cut(s)	962, 999, 1012, 1050, 1287, 1290, 1779, 1971, 2055
Sau3AI	GATC	9 cut(s)	42, 64, 76, 104, 640, 835, 1666, 1735, 1807
Sau96I	GGNCC	7 cut(s)	787, 818, 1321, 1612, 1919, 2062, 2063
SchI	GAGTC	1 cut(s)	1553
SduI	GDGCHC	4 cut(s)	807, 1641, 1681, 2066
SexAI	ACCWGGT	1 cut(s)	217
SfaNI	GCATC	5 cut(s)	1199, 1684, 1767, 1957, 2164
SinI	GGWCC	4 cut(s)	787, 818, 1612, 1919
SmaI	CCCGGG	1 cut(s)	1122
SmiMI	CAYNNNNRTG	5 cut(s)	410, 500, 984, 1661, 2318
SmlI	CTYRAG	1 cut(s)	974
SmoI	CTYRAG	1 cut(s)	974
SphI	GCATGC	1 cut(s)	875
SseBI	AGGCCT	1 cut(s)	1070
SspI	AATATT	3 cut(s)	1195, 1882, 2233
SspMI	CTAG	3 cut(s)	137, 2186, 2226
StuI	AGGCCT	1 cut(s)	1070
StyI	CCWWGG	7 cut(s)	51, 621, 955, 1097, 1662, 2058, 2341
TaiI	ACGT	3 cut(s)	1281, 1801, 2209
TaqI	TCGA	2 cut(s)	1413, 1965
TatI	WGTACW	2 cut(s)	1327, 1602
TfiI	GAWTC	5 cut(s)	32, 317, 752, 1573, 2112
Tru1I	TTAA	4 cut(s)	174, 975, 1709, 2082
Tru9I	TTAA	4 cut(s)	174, 975, 1709, 2082
TscAI	CASTG	7 cut(s)	34, 123, 307, 2094, 2284, 2305, 2320
TseFI	GTSAC	5 cut(s)	493, 841, 1718, 2260, 2266
TseI	GCWGC	9 cut(s)	961, 998, 1011, 1049, 1286, 1289, 1778, 1970, 2054
Tsp45I	GTSAC	5 cut(s)	493, 841, 1718, 2260, 2266
TspGWI	ACGGA	2 cut(s)	73, 2288
TspMI	CCCGGG	1 cut(s)	1120
TspRI	CASTG	7 cut(s)	34, 123, 307, 2094, 2284, 2305, 2320
Tth111I	GACNNNGTC	1 cut(s)	482
Van91I	CCANNNNNTGG	1 cut(s)	2049
Vha464I	CTTAAG	1 cut(s)	974
VpaK11BI	GGWCC	4 cut(s)	787, 818, 1612, 1919
XapI	RAATTY	3 cut(s)	140, 530, 1228
XceI	RCATGY	2 cut(s)	875, 1221
XmaI	CCCGGG	1 cut(s)	1120
XmiI	GTMKAC	1 cut(s)	1084
XmnI	GAANNNNTTC	1 cut(s)	316
XspI	CTAG	3 cut(s)	137, 2186, 2226
Zsp2I	ATGCAT	1 cut(s)	1223

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.