Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0009004

Physical Location & Seq

Genomic Coordinates Reverse (-)

12320321 .. 12327767

Gene Structure 7447 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00090620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2877 bp

ATGTATATAGCCACAGCCTCTTCCTCTGCCTCGGCTCTTCTCAGAGCTTCTAGGGCACGGTTCTTCTCCTCCTCCACTTCCACCTTGTCCAGAACCTTCTTGGCCGCGCCGCCCAAGCCTTCGTCGCATAGCTTCACCGCTCAGAGGCTTCTCAGCTCCTCGGCCGCCACTCGGTGGAGCCACGGCGTTCATTGGCGCGCTCCCTACACGCTTCGCTCTCAGATCAGAGCCATCGCTCCCGTCCTCGAACGCGTCCAGCGCAGGATCGCCACCATGGCTCCTGAGAGTCATCCATTCAAGGAGCATTTGACAGCTCTTCCCAAGCCAGGAGGCGGCGAGTATGGGAAGTTCTATAGCCTTACTTCACTGAAAGATCCGAGGATCGATAAGTTACCATACTCTATCAGAATCCTGCTTGAATCTGCTATTCGTAATTGTGACAACTTCCAAGTAAAGAAGGAAGATGTTGAGAAAATTCTTGACTGGGAAAATACTGCTCCAAAGCAAGTTGAAATTCCTTTCAAGCCTGCTCGTGTACTCTTGCAGGACTTTACAGGAGTGCCAGCTGTGGTTGACCTTGCTGTAATGCGTGATGCTATGAACAATCTTGGCAGTGATTCTAACAAGATCAATCCCTTGGTTCCTGTTGACCTTGTTGTTGATCACTCTGTTCAAGTCGATGTTGCAAGATCAGAAAATGCAGTGCAGGCAAATATGGAGCTAGAATTCCAGAGAAACAGGGAGAGATTTGCTTTTCTTAAATGGGGTTCTACCGCTTTCCAAAATATGCTTGTTGTTCCACCTGGTTCTGGCATTGTTCATCAGGTCAATCTTGAATATCTTGGACGGGTTGTTTTCAACACTAATGGCTTGCTGTACCCCGACAGTGTGGTTGGAACTGATTCCCATACAACCATGATTGATGGTTTGGGAGTAGCTGGCTGGGGAGTTGGGGGTATTGAAGCAGAAGCTACTATGCTTGGTCAGCCCATGAGCATGGTGTTGCCTGGAGTTGTTGGGTTCAAGTTATCTGGAAAGTTGAACAATGGTGTTACTGCTACCGACTTGGTCTTGACTGTCACACAAATGCTGAGGAAGCATGGAGTTGTGGGAAAATTTGTTGAGTTTTATGGGGGTGGTATGGGTGAACTGTCGTTAGCCGACAGGGCTACTATTGCCAATATGTCTCCTGAATATGGTGCAACCATGGGGTTCTTCCCAGTAGATCATGTTACTTTGCAATATCTCAAATTGACTGGTAGAAGTGATGAAACTGTGGCAATGATTGAAGGTTATCTTCGGGCAAATAAATTGTTTGTTGACTATAATGAGCCTGAATCAGAAAGAGTTTACTCATCTTACCTGGAATTGGACCTTTCACAAGTTGAGCCCTGTATTTCAGGACCAAAGAGACCTCATGATCGCGTGACCTTGAAAGATATGAAAGCCGATTGGCATGCCTGTCTTGATAACAAGGTCGGGTTCAAGGGATTTGCTATACCAAAAGAGGTACAAGACAAAGAGGTGAAGTTTTCATTCCATGGGCAGCCAGCACAACTTAAGCATGGTAGTGTTGTCATTGCTGCAATCACAAGCTGCACCAATACATCAAACCCAAGTGTCATGCTTGGGGCTGCTCTTGTTGCAAAAAAGGCCTGTGAACTTGGTCTACGGGGTTTCAATATTGTTGGATATGGATGCACGACATGCATTGGAAATTCAGGGGATTTAGATGAAAGTGTTGCTTCTGTCATAGCTGACAATGATATAGTAGCAGCTGCTGTGCTTTCAGGAAACAGGAACTTTGAGGGCCGTGTACATCCATTGACAAGGGCTAATTACCTTGCCTCACCTCCTTTAGTGGTTGCTTATGCCCTGGCTGGCACGGTTGACATTGATTTCGATAAGGAGCCAATTGGAACAGGCAAGGATGGTAAGAGCGTCTATTTCAGGGATATCTGGCCATCTTCAGAAGAGATTGCACAGGTTGTGCAATCCAGCGTGTTGCCAGAAATGTTCAGAAGCACTTATGAGTCTATTACTAAAGGCAACCCCATGTGGAATCAGCTGTCGGTTCCAGAAACCAAACTGTACTCTTGGGACCCTAACTCAACCTATATTCATGAGCCCCCATACTTCAAGGGCATGACCATGGATCCTCCTGGTGCTCATGGTGTGAAGGATGCCTACTGCCTGTTAAACTTTGGTGACAGTATTACAACAGATCACATCTCCCCAGCTGGAAGCATCCACAAGGACAGTCCTGCTGCAAAGTACCTTCTTGAACGCGGAGTGGATCGCAAGGACTTCAATTCCTATGGAAGTCGTCGTGGCAATGATGAAGTGATGGCAAGAGGAACTTTTGCCAATATTCGCCTTGTCAACAAGCTATTGAATGGAGAAGTGGGTCCCAAAACAGTTCACATTCCTTCCGGAGAGAAACTCTCTGTGTTTGATGCAGCAACGAAATACAAGTCTGCTGGGCAGGACACCATTGTTTTGGCTGGTGCTGAGTATGGAAGTGGAAGCTCCAGGGATTGGGCTGCCAAGGGCCCAATGTTACTGGGTGTTAAAGCAGTGATTGCTAAAAGTTTTGAGAGAATCCATCGCAGTAATTTGGTGGGTATGGGTATTATTCCACTTTGTTTCAAAGCTGGTGAGGATGCAGATACACTAGGATTGACTGGTCATGAACGTTATACCATTGACCTCCCTAGCAATATTAGTGAGATAAAGCCTGGCCAAGATGTGACTGTCACCACGGACAGTGGAAAGTCTTTTGTATGCACTGTGCGCTTCGACACTGAGGTGGAATTGGCCTACTTCAACCATGGAGGGATTCTTCCATATGTGATAAGGAACCTGAGCAAGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001101 GO:0003674 GO:0003824 GO:0003994 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005829 GO:0006082 GO:0006101 GO:0006102 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031090 GO:0031667 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0033993 GO:0035639 GO:0036094 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048856 GO:0050896 GO:0071704 GO:0071944 GO:0072350 GO:0090351 GO:0097159 GO:0097305 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1901700 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Aconitase Aconitase_C

Protein Analysis

958

Amino Acids

104.42

Weight (kDa)

6.78

Isoelectric Point (pI)

34.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aconitase	PF00330	157 - 557	2.3e-126	Aconitase family (aconitate hydratase)
Aconitase	PF00330	559 - 628	1.1e-19	Aconitase family (aconitate hydratase)
Aconitase_C	PF00694	757 - 885	4.7e-41	Aconitase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G05710
fragaria_vesca	FvH4_6g43190
malus_domestica	MD09G1108800.v1.1 MD17G1096700.v1.1
prunus_persica	Prupe.3G217300_v2.0.a1 Prupe.3G217300_v2.0.a1
pyrus_communis	pycom09g03120 pycom17g09260
rosa_chinensis	RchiOBHm_Chr2g0159291
rosa_laevigata	RLG00000021152
rosa_multiflora	Rmu_sc0004800.1_g000001 Rmu_sc0007806.1_g000010 Rmu_sc0010590.1_g000001
rosa_roxburghii	Rroxscaffold_2G00090620
rosa_rugosa	Rorug02G0475700
rosa_samantha	Rh2AG542100 Rh2BG554700 Rh2CG524800 Rh2DG564400 Rh5AG522200
rosa_wichuraiana	Rw2G044840 Rw5G048460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	174, 2538
AccBSI	CCGCTC	1 cut(s)	140
AccI	GTMKAC	1 cut(s)	1669
AccII	CGCG	5 cut(s)	107, 198, 252, 1425, 2289
AccIII	TCCGGA	1 cut(s)	2432
AciI	CCGC	7 cut(s)	105, 110, 138, 165, 333, 774, 2289
AclI	AACGTT	1 cut(s)	2695
AclWI	GGATC	6 cut(s)	272, 368, 389, 2150, 2163, 2304
AcoI	YGGCCR	4 cut(s)	102, 162, 1961, 2740
AcsI	RAATTY	5 cut(s)	474, 513, 725, 1115, 1717
AcuI	CTGAAG	1 cut(s)	1953
AdeI	CACNNNGTG	1 cut(s)	174
AfaI	GTAC	6 cut(s)	537, 878, 1512, 1818, 2093, 2276
AfiI	CCNNNNNNNGG	9 cut(s)	144, 171, 174, 326, 332, 809, 1196, 1369, 2538
AflII	CTTAAG	1 cut(s)	1559
AflIII	ACRYGT	1 cut(s)	250
AjnI	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1875, 2161, 2531, 2737
AleI	CACNNNNGTG	1 cut(s)	2807
Alw21I	GWGCWC	1 cut(s)	2170
Alw26I	GTCTC	2 cut(s)	1191, 1405
AlwI	GGATC	6 cut(s)	272, 368, 389, 2150, 2163, 2304
AlwNI	CAGNNNCTG	1 cut(s)	1781
Aor13HI	TCCGGA	1 cut(s)	2432
AoxI	GGCC	8 cut(s)	102, 162, 1653, 1810, 1961, 2551, 2740, 2817
ApaI	GGGCCC	1 cut(s)	2555
ApeKI	GCWGC	9 cut(s)	1546, 1583, 1596, 1634, 1775, 1778, 2267, 2459, 2543
ApoI	RAATTY	5 cut(s)	474, 513, 725, 1115, 1717
ArsI	GACNNNNNNTTYG	4 cut(s)	1883, 1915, 2459, 2491
Asp700I	GAANNNNTTC	1 cut(s)	901
AspLEI	GCGC	5 cut(s)	109, 198, 200, 261, 2796
AspS9I	GGNCC	7 cut(s)	1372, 1403, 1810, 2101, 2408, 2551, 2552
AsuHPI	GGTGA	7 cut(s)	127, 1157, 1537, 1842, 2219, 2669, 2749
AvaII	GGWCC	4 cut(s)	1372, 1403, 2101, 2408
BaeGI	GKGCMC	2 cut(s)	58, 2555
BalI	TGGCCA	2 cut(s)	1963, 2742
BamHI	GGATCC	1 cut(s)	2155
BanII	GRGCYC	3 cut(s)	1392, 2130, 2555
BarI	GAAGNNNNNNTAC	2 cut(s)	2171, 2203
BauI	CACGAG	1 cut(s)	531
Bbv12I	GWGCWC	1 cut(s)	2170
BbvCI	CCTCAGC	1 cut(s)	1091
BbvI	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1765, 1787, 2254, 2471, 2530
BccI	CCATC	6 cut(s)	239, 917, 1925, 1972, 2341, 2613
BceAI	ACGGC	2 cut(s)	199, 1797
BcgI	CGANNNNNNTGC	2 cut(s)	1439, 1473
BciT130I	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
BclI	TGATCA	1 cut(s)	661
BcoDI	GTCTC	2 cut(s)	1191, 1405
BfaI	CTAG	4 cut(s)	51, 722, 2675, 2715
BfmI	CTRYAG	1 cut(s)	352
BfrI	CTTAAG	1 cut(s)	1559
BglI	GCCNNNNNGGC	2 cut(s)	275, 1166
Bme1390I	CCNGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
Bme18I	GGWCC	4 cut(s)	1372, 1403, 2101, 2408
BmgT120I	GGNCC	7 cut(s)	1372, 1403, 1810, 2101, 2408, 2551, 2552
BmrFI	CCNGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
BmrI	ACTGGG	3 cut(s)	493, 1214, 2573
BmsI	GCATC	6 cut(s)	583, 1688, 2173, 2256, 2446, 2653
BmuI	ACTGGG	3 cut(s)	493, 1214, 2573
BplI	GAGNNNNNCTC	4 cut(s)	2427, 2459, 2429, 2461
BpmI	CTGGAG	2 cut(s)	1029, 2515
Bpu10I	CCTNAGC	2 cut(s)	1091, 2864
Bsa29I	ATCGAT	1 cut(s)	384
BsaI	GGTCTC	1 cut(s)	1405
BsaWI	WCCGGW	1 cut(s)	2432
BsaXI	ACNNNNNCTCC	2 cut(s)	262, 292
Bsc4I	CCNNNNNNNGG	9 cut(s)	144, 171, 174, 326, 332, 809, 1196, 1369, 2538
Bse1I	ACTGG	5 cut(s)	488, 1220, 1261, 2568, 2689
Bse3DI	GCAATG	3 cut(s)	1287, 1578, 2341
BseAI	TCCGGA	1 cut(s)	2432
BseBI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
BseCI	ATCGAT	1 cut(s)	384
BseGI	GGATG	7 cut(s)	289, 1703, 1819, 1936, 2188, 2247, 2668
BseLI	CCNNNNNNNGG	9 cut(s)	144, 171, 174, 326, 332, 809, 1196, 1369, 2538
BseMI	GCAATG	3 cut(s)	1287, 1578, 2341
BseMII	CTCAG	9 cut(s)	55, 155, 166, 233, 273, 1082, 2502, 2796, 2855
BseNI	ACTGG	5 cut(s)	488, 1220, 1261, 2568, 2689
BsePI	GCGCGC	1 cut(s)	196
BseRI	GAGGAG	3 cut(s)	58, 61, 148
BseSI	GKGCMC	2 cut(s)	58, 2555
BseX3I	CGGCCG	1 cut(s)	162
BseXI	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1765, 1787, 2254, 2471, 2530
BseYI	CCCAGC	3 cut(s)	942, 2236, 2480
BsgI	GTGCAG	2 cut(s)	725, 1582
Bsh1236I	CGCG	5 cut(s)	107, 198, 252, 1425, 2289
Bsh1285I	CGRYCG	1 cut(s)	165
BshFI	GGCC	8 cut(s)	104, 164, 1655, 1812, 1963, 2553, 2742, 2819
BshVI	ATCGAT	1 cut(s)	384
BsiEI	CGRYCG	1 cut(s)	165
BsiHKAI	GWGCWC	1 cut(s)	2170
BsiSI	CCGG	1 cut(s)	2433
BslFI	GGGAC	2 cut(s)	2114, 2394
BslI	CCNNNNNNNGG	9 cut(s)	144, 171, 174, 326, 332, 809, 1196, 1369, 2538
BsmAI	GTCTC	2 cut(s)	1191, 1405
BsmFI	GGGAC	2 cut(s)	2114, 2394
BsnI	GGCC	8 cut(s)	104, 164, 1655, 1812, 1963, 2553, 2742, 2819
Bso31I	GGTCTC	1 cut(s)	1405
Bsp120I	GGGCCC	1 cut(s)	2551
Bsp1286I	GDGCHC	5 cut(s)	58, 1392, 2130, 2170, 2555
Bsp13I	TCCGGA	1 cut(s)	2432
Bsp1407I	TGTACA	1 cut(s)	1816
Bsp19I	CCATGG	5 cut(s)	273, 1206, 1540, 2151, 2830
BspACI	CCGC	7 cut(s)	105, 110, 138, 165, 333, 774, 2289
BspANI	GGCC	8 cut(s)	104, 164, 1655, 1812, 1963, 2553, 2742, 2819
BspCNI	CTCAG	9 cut(s)	54, 154, 165, 232, 274, 1083, 2503, 2797, 2856
BspDI	ATCGAT	1 cut(s)	384
BspEI	TCCGGA	1 cut(s)	2432
BspFNI	CGCG	5 cut(s)	107, 198, 252, 1425, 2289
BspHI	TCATGA	3 cut(s)	1417, 2122, 2689
BspPI	GGATC	6 cut(s)	272, 368, 389, 2150, 2163, 2304
BspQI	GCTCTTC	2 cut(s)	42, 321
BspTI	CTTAAG	1 cut(s)	1559
BspTNI	GGTCTC	1 cut(s)	1405
BsrBI	CCGCTC	1 cut(s)	140
BsrDI	GCAATG	3 cut(s)	1287, 1578, 2341
BsrGI	TGTACA	1 cut(s)	1816
BsrI	ACTGG	5 cut(s)	488, 1220, 1261, 2568, 2689
BssHII	GCGCGC	1 cut(s)	196
BssSI	CACGAG	1 cut(s)	531
BssT1I	CCWWGG	7 cut(s)	273, 636, 1206, 1540, 2151, 2547, 2830
Bst2BI	CACGAG	1 cut(s)	531
Bst2UI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
Bst6I	CTCTTC	4 cut(s)	25, 42, 321, 1968
BstAFI	CTTAAG	1 cut(s)	1559
BstAPI	GCANNNNNTGC	2 cut(s)	1707, 2582
BstAUI	TGTACA	1 cut(s)	1816
BstDEI	CTNAG	9 cut(s)	41, 141, 152, 219, 282, 1091, 2511, 2805, 2864
BstDSI	CCRYGG	7 cut(s)	181, 273, 1206, 1540, 2151, 2760, 2830
BstF5I	GGATG	7 cut(s)	289, 1703, 1819, 1936, 2188, 2247, 2668
BstFNI	CGCG	5 cut(s)	107, 198, 252, 1425, 2289
BstHHI	GCGC	5 cut(s)	109, 198, 200, 261, 2796
BstMAI	GTCTC	2 cut(s)	1191, 1405
BstMCI	CGRYCG	1 cut(s)	165
BstNI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
BstNSI	RCATGY	2 cut(s)	1460, 1710
BstSCI	CCNGG	8 cut(s)	325, 802, 1006, 1362, 1875, 2161, 2531, 2737
BstSFI	CTRYAG	1 cut(s)	352
BstSLI	GKGCMC	2 cut(s)	58, 2555
BstUI	CGCG	5 cut(s)	107, 198, 252, 1425, 2289
BstV1I	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1765, 1787, 2254, 2471, 2530
BstX2I	RGATCY	2 cut(s)	373, 2155
BstXI	CCANNNNNNTGG	2 cut(s)	997, 2500
BstYI	RGATCY	2 cut(s)	373, 2155
BstZI	CGGCCG	1 cut(s)	162
Bsu15I	ATCGAT	1 cut(s)	384
BsuRI	GGCC	8 cut(s)	104, 164, 1655, 1812, 1963, 2553, 2742, 2819
BsuTUI	ATCGAT	1 cut(s)	384
BtgI	CCRYGG	7 cut(s)	181, 273, 1206, 1540, 2151, 2760, 2830
BtgZI	GCGATG	2 cut(s)	217, 2591
BtsCI	GGATG	7 cut(s)	289, 1703, 1819, 1936, 2188, 2247, 2668
BtsI	GCAGTG	3 cut(s)	619, 708, 2583
BtsIMutI	CAGTG	8 cut(s)	365, 619, 708, 892, 2583, 2773, 2787, 2802
CaiI	CAGNNNCTG	1 cut(s)	1781
CciI	TCATGA	3 cut(s)	1417, 2122, 2689
CfoI	GCGC	5 cut(s)	109, 198, 200, 261, 2796
Cfr13I	GGNCC	7 cut(s)	1372, 1403, 1810, 2101, 2408, 2551, 2552
ClaI	ATCGAT	1 cut(s)	384
CseI	GACGC	2 cut(s)	241, 1930
CsiI	ACCWGGT	1 cut(s)	802
Csp6I	GTAC	6 cut(s)	536, 877, 1511, 1817, 2092, 2275
CviQI	GTAC	6 cut(s)	536, 877, 1511, 1817, 2092, 2275
DdeI	CTNAG	9 cut(s)	41, 141, 152, 219, 282, 1091, 2511, 2805, 2864
DraIII	CACNNNGTG	1 cut(s)	174
EaeI	YGGCCR	4 cut(s)	102, 162, 1961, 2740
EagI	CGGCCG	1 cut(s)	162
Eam1104I	CTCTTC	4 cut(s)	25, 42, 321, 1968
EarI	CTCTTC	4 cut(s)	25, 42, 321, 1968
EclXI	CGGCCG	1 cut(s)	162
Eco130I	CCWWGG	7 cut(s)	273, 636, 1206, 1540, 2151, 2547, 2830
Eco147I	AGGCCT	1 cut(s)	1655
Eco24I	GRGCYC	3 cut(s)	1392, 2130, 2555
Eco31I	GGTCTC	1 cut(s)	1405
Eco32I	GATATC	1 cut(s)	1957
Eco47I	GGWCC	4 cut(s)	1372, 1403, 2101, 2408
Eco52I	CGGCCG	1 cut(s)	162
Eco57I	CTGAAG	1 cut(s)	1953
EcoO109I	RGGNCCY	3 cut(s)	2101, 2408, 2551
EcoRI	GAATTC	1 cut(s)	725
EcoRII	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1875, 2161, 2531, 2737
EcoRV	GATATC	1 cut(s)	1957
EcoT14I	CCWWGG	7 cut(s)	273, 636, 1206, 1540, 2151, 2547, 2830
EcoT22I	ATGCAT	1 cut(s)	1712
EcoT38I	GRGCYC	3 cut(s)	1392, 2130, 2555
ErhI	CCWWGG	7 cut(s)	273, 636, 1206, 1540, 2151, 2547, 2830
FalI	AAGNNNNNCTT	6 cut(s)	1281, 1313, 1729, 1761, 2344, 2376
FaqI	GGGAC	2 cut(s)	2114, 2394
FauNDI	CATATG	1 cut(s)	2848
FbaI	TGATCA	1 cut(s)	661
FblI	GTMKAC	1 cut(s)	1669
FokI	GGATG	7 cut(s)	276, 1710, 1806, 1943, 2195, 2234, 2675
FriOI	GRGCYC	3 cut(s)	1392, 2130, 2555
FspBI	CTAG	4 cut(s)	51, 722, 2675, 2715
GlaI	GCGC	5 cut(s)	108, 197, 199, 260, 2795
GsaI	CCCAGC	3 cut(s)	946, 2240, 2484
GsuI	CTGGAG	2 cut(s)	1029, 2515
HaeIII	GGCC	8 cut(s)	104, 164, 1655, 1812, 1963, 2553, 2742, 2819
HapII	CCGG	1 cut(s)	2433
HgaI	GACGC	2 cut(s)	241, 1930
HhaI	GCGC	5 cut(s)	109, 198, 200, 261, 2796
Hin6I	GCGC	5 cut(s)	107, 196, 198, 259, 2794
HinP1I	GCGC	5 cut(s)	107, 196, 198, 259, 2794
HincII	GTYRAC	5 cut(s)	574, 649, 1321, 1891, 2383
HindII	GTYRAC	5 cut(s)	574, 649, 1321, 1891, 2383
HpaII	CCGG	1 cut(s)	2433
HphI	GGTGA	7 cut(s)	127, 1157, 1537, 1842, 2219, 2669, 2749
Hpy99I	CGWCG	2 cut(s)	127, 2331
HpyAV	CCTTC	7 cut(s)	106, 129, 451, 1283, 2173, 2288, 2439
HpyCH4IV	ACGT	1 cut(s)	2695
HpyF3I	CTNAG	9 cut(s)	41, 141, 152, 219, 282, 1091, 2511, 2805, 2864
HpySE526I	ACGT	1 cut(s)	2695
HspAI	GCGC	5 cut(s)	107, 196, 198, 259, 2794
KflI	GGGWCCC	2 cut(s)	2101, 2408
Kpn2I	TCCGGA	1 cut(s)	2432
Ksp22I	TGATCA	1 cut(s)	661
LguI	GCTCTTC	2 cut(s)	42, 321
Lsp1109I	GCAGC	9 cut(s)	1558, 1570, 1583, 1621, 1765, 1787, 2254, 2471, 2530
LweI	GCATC	6 cut(s)	583, 1688, 2173, 2256, 2446, 2653
MabI	ACCWGGT	1 cut(s)	802
MaeI	CTAG	4 cut(s)	51, 722, 2675, 2715
MaeII	ACGT	1 cut(s)	2695
MbiI	CCGCTC	1 cut(s)	140
MfeI	CAATTG	1 cut(s)	1914
MflI	RGATCY	2 cut(s)	373, 2155
MhlI	GDGCHC	5 cut(s)	58, 1392, 2130, 2170, 2555
MlsI	TGGCCA	2 cut(s)	1963, 2742
MluI	ACGCGT	1 cut(s)	250
MluNI	TGGCCA	2 cut(s)	1963, 2742
MlyI	GAGTC	2 cut(s)	295, 2042
MmeI	TCCRAC	2 cut(s)	874, 1669
Mox20I	TGGCCA	2 cut(s)	1963, 2742
Mph1103I	ATGCAT	1 cut(s)	1712
MroI	TCCGGA	1 cut(s)	2432
MroXI	GAANNNNTTC	1 cut(s)	901
MscI	TGGCCA	2 cut(s)	1963, 2742
MseI	TTAA	4 cut(s)	759, 1560, 2198, 2571
MslI	CAYNNNNRTG	5 cut(s)	995, 1085, 1569, 2150, 2807
Msp20I	TGGCCA	2 cut(s)	1963, 2742
MspA1I	CMGCKG	4 cut(s)	566, 1778, 2068, 2240
MspCI	CTTAAG	1 cut(s)	1559
MspI	CCGG	1 cut(s)	2433
MspR9I	CCNGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
MunI	CAATTG	1 cut(s)	1914
MvaI	CCWGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
MvnI	CGCG	5 cut(s)	107, 198, 252, 1425, 2289
NcoI	CCATGG	5 cut(s)	273, 1206, 1540, 2151, 2830
NdeI	CATATG	1 cut(s)	2848
NmeAIII	GCCGAG	2 cut(s)	11, 140
NmuCI	GTSAC	6 cut(s)	437, 1078, 1426, 2207, 2749, 2755
NsiI	ATGCAT	1 cut(s)	1712
NspI	RCATGY	2 cut(s)	1460, 1710
OliI	CACNNNNGTG	1 cut(s)	2807
PaeI	GCATGC	1 cut(s)	1460
PagI	TCATGA	3 cut(s)	1417, 2122, 2689
PauI	GCGCGC	1 cut(s)	196
PceI	AGGCCT	1 cut(s)	1655
PciSI	GCTCTTC	2 cut(s)	42, 321
PdmI	GAANNNNTTC	1 cut(s)	901
PfeI	GAWTC	8 cut(s)	408, 419, 617, 902, 1337, 2062, 2601, 2839
PflFI	GACNNNGTC	1 cut(s)	1067
PflMI	CCANNNNNTGG	2 cut(s)	174, 2538
PleI	GAGTC	2 cut(s)	294, 2041
PpsI	GAGTC	2 cut(s)	294, 2041
PpuMI	RGGWCCY	2 cut(s)	2101, 2408
Psp1406I	AACGTT	1 cut(s)	2695
Psp5II	RGGWCCY	2 cut(s)	2101, 2408
Psp6I	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1875, 2161, 2531, 2737
PspFI	CCCAGC	3 cut(s)	942, 2236, 2480
PspGI	CCWGG	8 cut(s)	325, 802, 1006, 1362, 1875, 2161, 2531, 2737
PspOMI	GGGCCC	1 cut(s)	2551
PspPI	GGNCC	7 cut(s)	1372, 1403, 1810, 2101, 2408, 2551, 2552
PspPPI	RGGWCCY	2 cut(s)	2101, 2408
PsrI	GAACNNNNNNTAC	4 cut(s)	332, 364, 1653, 1685
PstNI	CAGNNNCTG	1 cut(s)	1781
PsuI	RGATCY	2 cut(s)	373, 2155
PsyI	GACNNNGTC	1 cut(s)	1067
PteI	GCGCGC	1 cut(s)	196
PvuII	CAGCTG	4 cut(s)	566, 1778, 2068, 2240
RsaI	GTAC	6 cut(s)	537, 878, 1512, 1818, 2093, 2276
RsaNI	GTAC	6 cut(s)	536, 877, 1511, 1817, 2092, 2275
RseI	CAYNNNNRTG	5 cut(s)	995, 1085, 1569, 2150, 2807
SapI	GCTCTTC	2 cut(s)	42, 321
SaqAI	TTAA	4 cut(s)	759, 1560, 2198, 2571
Sau96I	GGNCC	7 cut(s)	1372, 1403, 1810, 2101, 2408, 2551, 2552
SchI	GAGTC	2 cut(s)	295, 2042
ScrFI	CCNGG	8 cut(s)	327, 804, 1008, 1364, 1877, 2163, 2533, 2739
SduI	GDGCHC	5 cut(s)	58, 1392, 2130, 2170, 2555
SexAI	ACCWGGT	1 cut(s)	802
SfaNI	GCATC	6 cut(s)	583, 1688, 2173, 2256, 2446, 2653
SfcI	CTRYAG	1 cut(s)	352
SinI	GGWCC	4 cut(s)	1372, 1403, 2101, 2408
SmiMI	CAYNNNNRTG	5 cut(s)	995, 1085, 1569, 2150, 2807
SmlI	CTYRAG	1 cut(s)	1559
SmoI	CTYRAG	1 cut(s)	1559
SphI	GCATGC	1 cut(s)	1460
SseBI	AGGCCT	1 cut(s)	1655
SsiI	CCGC	7 cut(s)	105, 110, 138, 165, 333, 774, 2289
SspI	AATATT	3 cut(s)	1684, 2371, 2722
SspMI	CTAG	4 cut(s)	51, 722, 2675, 2715
StuI	AGGCCT	1 cut(s)	1655
StyD4I	CCNGG	8 cut(s)	325, 802, 1006, 1362, 1875, 2161, 2531, 2737
StyI	CCWWGG	7 cut(s)	273, 636, 1206, 1540, 2151, 2547, 2830
TaiI	ACGT	1 cut(s)	2698
TaqI	TCGA	5 cut(s)	246, 384, 678, 1902, 2799
TatI	WGTACW	3 cut(s)	535, 1816, 2091
TauI	GCSGC	4 cut(s)	107, 112, 167, 336
TfiI	GAWTC	8 cut(s)	408, 419, 617, 902, 1337, 2062, 2601, 2839
Tru1I	TTAA	4 cut(s)	759, 1560, 2198, 2571
Tru9I	TTAA	4 cut(s)	759, 1560, 2198, 2571
TscAI	CASTG	8 cut(s)	372, 619, 708, 892, 2583, 2773, 2794, 2809
TseFI	GTSAC	6 cut(s)	437, 1078, 1426, 2207, 2749, 2755
TseI	GCWGC	9 cut(s)	1546, 1583, 1596, 1634, 1775, 1778, 2267, 2459, 2543
Tsp45I	GTSAC	6 cut(s)	437, 1078, 1426, 2207, 2749, 2755
TspGWI	ACGGA	1 cut(s)	2777
TspRI	CASTG	8 cut(s)	372, 619, 708, 892, 2583, 2773, 2794, 2809
Tth111I	GACNNNGTC	1 cut(s)	1067
Van91I	CCANNNNNTGG	2 cut(s)	174, 2538
Vha464I	CTTAAG	1 cut(s)	1559
VpaK11BI	GGWCC	4 cut(s)	1372, 1403, 2101, 2408
XapI	RAATTY	5 cut(s)	474, 513, 725, 1115, 1717
XceI	RCATGY	2 cut(s)	1460, 1710
XcmI	CCANNNNNNNNNTGG	1 cut(s)	97
XmiI	GTMKAC	1 cut(s)	1669
XmnI	GAANNNNTTC	1 cut(s)	901
XspI	CTAG	4 cut(s)	51, 722, 2675, 2715
Zsp2I	ATGCAT	1 cut(s)	1712

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.