MD17G1263500.v1.1

zinc-RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010929
Physical Location & Seq
Forward (+)
32444644 .. 32445892
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1263500.v1.1.491

Sequence Viewer

Length: 681 bp
ATGAGCAGAAGTACCAACAATACCTATTCTCTTCCTACTCCTCCTCGTCCTTATCTTACCTATCGTGAGCCGTTTGATCTCTACTTCGGGAATTTTGAGGACGATTTTGGTGAATGGGCTTCTGGGATCTCTGGTAGTCCCTTGTATGATTTTCAAGCAACTCAAGTAACCAGAACAGGTGCGTCGGATTCAAGGCGACCATTTGAGGGAACGCTGGCGGTTGCAAGACCTCTTGGCTTAGCAGGTTCAGCAGCAGCACCAGGTCCCCGAGTTCATATTCTGGTCCTTGGCGATGAATTGGCACGACAGGCAGACCCAAGGGCTAGACAGATGGTCGGTAACCAATGGAATACGGCCGCCCCAGGACTTGAAGCAGACTCCAGATTGACCCATAACGAGCAAAAGAAGGCGTTGCAGAAGCTGAAGAAAGAGATATACAATCCTTTACGGAGGAGAATATCGCAGAGAGTGAACTTGTATTACAGAGACATAGCTGTGAAGGAGAAGGAAATGGAGAATGATGAAAATGGCAAGAGGTGTCCCATTTGCTTGGATGACTTTGAGCCCAAACAAGAGGTTATGCTCACTCCTTGTAAGCATATGTTTCATGAAGAATGCATTGTGCCTTGGGTGAAGAGCAATGGTCGTTGCCCAGTATGTAGGTTTTCTATATCTGAGTGA

Protein Analysis

227

Amino Acids

25.67

Weight (kDa)

8.19

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 178 - 225 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 179 - 221 7.4e-16 Ring finger domain
zf-C3HC4_2 PF13923 179 - 220 9.7e-11 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 179 - 221 7e-09 zinc-RING finger domain
zf-rbx1 PF12678 179 - 221 3.4e-08 RING-H2 zinc finger domain
zf-RING_11 PF17123 180 - 207 3.3e-12 RING-like zinc finger
zf-C3HC4 PF00097 180 - 220 6.9e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 180 - 218 3.7e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58720 AT3G58720 AT3G58720 AT3G58720
fragaria_vesca FvH4_6g27790
malus_domestica MD09G1234400.v1.1 MD17G1263500.v1.1
prunus_persica Prupe.3G112600_v2.0.a1
pyrus_communis pycom09g15270 pycom17g26370
rosa_chinensis RchiOBHm_Chr2g0131091
rosa_laevigata RLG00000019166
rosa_multiflora Rmu_sc0003629.1_g000064
rosa_roxburghii Rroxscaffold_2G00113210
rosa_rugosa Rorug02G0294900
rosa_samantha Rh2AG347200 Rh2BG354700 Rh2CG333400 Rh2DG372600
rosa_wichuraiana Rw2G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 233
AciI CCGC 2 cut(s) 218, 357
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 3 cut(s) 155, 192, 371
AhdI GACNNNNNGTC 1 cut(s) 332
AjnI CCWGG 2 cut(s) 259, 361
AluBI AGCT 2 cut(s) 421, 494
AluI AGCT 2 cut(s) 421, 494
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 421
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 2 cut(s) 251, 254
ApoI RAATTY 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 263, 283
AsuHPI GGTGA 2 cut(s) 122, 643
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 2 cut(s) 263, 283
BanII GRGCYC 1 cut(s) 567
BbvI GCAGC 2 cut(s) 263, 266
BccI CCATC 1 cut(s) 325
BceAI ACGGC 2 cut(s) 55, 369
BciT130I CCWGG 2 cut(s) 261, 363
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 233
BisI GCNGC 3 cut(s) 252, 255, 357
BlpI GCTNAGC 1 cut(s) 238
BlsI GCNGC 3 cut(s) 253, 256, 358
Bme1390I CCNGG 2 cut(s) 261, 363
Bme18I GGWCC 2 cut(s) 263, 283
BmeRI GACNNNNNGTC 1 cut(s) 332
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 2 cut(s) 263, 283
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 2 cut(s) 261, 363
BmrI ACTGGG 1 cut(s) 647
BmuI ACTGGG 1 cut(s) 647
BpmI CTGGAG 1 cut(s) 364
Bpu1102I GCTNAGC 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 147
BsaJI CCNNGG 4 cut(s) 286, 317, 361, 626
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 653
Bse3DI GCAATG 1 cut(s) 646
BseBI CCWGG 2 cut(s) 261, 363
BseDI CCNNGG 4 cut(s) 286, 317, 361, 626
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 646
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 1 cut(s) 653
BseRI GAGGAG 3 cut(s) 30, 33, 466
BseX3I CGGCCG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 263, 266
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 357
BsiHKCI CYCGRG 1 cut(s) 267
BslFI GGGAC 3 cut(s) 123, 249, 525
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 3 cut(s) 123, 249, 525
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 567
Bsp143I GATC 2 cut(s) 76, 126
Bsp1720I GCTNAGC 1 cut(s) 238
BspACI CCGC 2 cut(s) 218, 357
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 1 cut(s) 667
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 265
BspMI ACCTGC 1 cut(s) 233
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 646
BsrI ACTGG 1 cut(s) 653
BssECI CCNNGG 4 cut(s) 286, 317, 361, 626
BssMI GATC 2 cut(s) 76, 126
BssT1I CCWWGG 3 cut(s) 286, 317, 626
Bst2UI CCWGG 2 cut(s) 261, 363
Bst6I CTCTTC 2 cut(s) 36, 629
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 2 cut(s) 238, 675
BstEII GGTNACC 1 cut(s) 338
BstF5I GGATG 1 cut(s) 559
BstKTI GATC 2 cut(s) 79, 129
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 76, 126
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 2 cut(s) 248, 308
BstNI CCWGG 2 cut(s) 261, 363
BstPI GGTNACC 1 cut(s) 338
BstSCI CCNGG 2 cut(s) 259, 361
BstV1I GCAGC 2 cut(s) 263, 266
BstX2I RGATCY 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 550
BstYI RGATCY 1 cut(s) 126
BstZI CGGCCG 1 cut(s) 354
BsuRI GGCC 1 cut(s) 356
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 559
BveI ACCTGC 1 cut(s) 233
Cac8I GCNNGC 1 cut(s) 216
CaiI CAGNNNCTG 1 cut(s) 421
CciI TCATGA 1 cut(s) 607
Cfr13I GGNCC 2 cut(s) 263, 283
CseI GACGC 1 cut(s) 171
CsiI ACCWGGT 1 cut(s) 259
Csp6I GTAC 1 cut(s) 12
CviAII CATG 1 cut(s) 608
CviJI RGCY 8 cut(s) 70, 119, 237, 323, 356, 421, 494, 565
CviKI_1 RGCY 8 cut(s) 70, 119, 237, 323, 356, 421, 494, 565
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 2 cut(s) 238, 675
DpnI GATC 2 cut(s) 78, 128
DpnII GATC 2 cut(s) 76, 126
DriI GACNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 354
EagI CGGCCG 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 36, 629
Eam1105I GACNNNNNGTC 1 cut(s) 332
EarI CTCTTC 2 cut(s) 36, 629
EclXI CGGCCG 1 cut(s) 354
Eco130I CCWWGG 3 cut(s) 286, 317, 626
Eco24I GRGCYC 1 cut(s) 567
Eco47I GGWCC 2 cut(s) 263, 283
Eco52I CGGCCG 1 cut(s) 354
Eco57I CTGAAG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 267
Eco91I GGTNACC 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 338
EcoRII CCWGG 2 cut(s) 259, 361
EcoT14I CCWWGG 3 cut(s) 286, 317, 626
EcoT22I ATGCAT 1 cut(s) 620
EcoT38I GRGCYC 1 cut(s) 567
ErhI CCWWGG 3 cut(s) 286, 317, 626
FaeI CATG 1 cut(s) 611
FaqI GGGAC 3 cut(s) 123, 249, 525
FatI CATG 1 cut(s) 607
FauNDI CATATG 1 cut(s) 600
Fnu4HI GCNGC 3 cut(s) 252, 255, 357
FokI GGATG 1 cut(s) 566
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 3 cut(s) 252, 255, 357
FspBI CTAG 1 cut(s) 324
GluI GCNGC 3 cut(s) 252, 255, 357
GsuI CTGGAG 1 cut(s) 364
HaeIII GGCC 1 cut(s) 356
HgaI GACGC 1 cut(s) 171
Hin1II CATG 1 cut(s) 611
HinfI GANTC 2 cut(s) 188, 377
HphI GGTGA 2 cut(s) 122, 643
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 2 cut(s) 187, 676
Hpy188III TCNNGA 4 cut(s) 65, 88, 381, 608
Hpy8I GTNNAC 1 cut(s) 472
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 3 cut(s) 400, 493, 499
HpyCH4V TGCA 3 cut(s) 224, 415, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 308
HpyF3I CTNAG 2 cut(s) 238, 675
Hsp92II CATG 1 cut(s) 611
Kzo9I GATC 2 cut(s) 76, 126
LguI GCTCTTC 1 cut(s) 629
Lsp1109I GCAGC 2 cut(s) 263, 266
MabI ACCWGGT 1 cut(s) 259
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 166, 338
MalI GATC 2 cut(s) 78, 128
MboI GATC 2 cut(s) 76, 126
MboII GAAGA 4 cut(s) 23, 436, 623, 646
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 2 cut(s) 91, 296
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 8 cut(s) 51, 54, 91, 199, 240, 444, 528, 568
Mph1103I ATGCAT 1 cut(s) 620
MslI CAYNNNNRTG 1 cut(s) 494
MspR9I CCNGG 2 cut(s) 261, 363
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 2 cut(s) 261, 363
MwoI GCNNNNNNNGC 2 cut(s) 248, 308
NdeI CATATG 1 cut(s) 600
NdeII GATC 2 cut(s) 76, 126
NlaIII CATG 1 cut(s) 611
NlaIV GGNNCC 1 cut(s) 265
NsiI ATGCAT 1 cut(s) 620
PagI TCATGA 1 cut(s) 607
PciSI GCTCTTC 1 cut(s) 629
PctI GAATGC 1 cut(s) 620
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 3 cut(s) 253, 256, 358
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
PpuMI RGGWCCY 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 259, 361
PspEI GGTNACC 1 cut(s) 338
PspGI CCWGG 2 cut(s) 259, 361
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 263, 283
PspPPI RGGWCCY 1 cut(s) 263
PsrI GAACNNNNNNTAC 2 cut(s) 464, 496
PstNI CAGNNNCTG 1 cut(s) 421
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 1 cut(s) 494
SapI GCTCTTC 1 cut(s) 629
SatI GCNGC 3 cut(s) 252, 255, 357
Sau3AI GATC 2 cut(s) 76, 126
Sau96I GGNCC 2 cut(s) 263, 283
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 2 cut(s) 261, 363
SduI GDGCHC 1 cut(s) 567
SexAI ACCWGGT 1 cut(s) 259
SinI GGWCC 2 cut(s) 263, 283
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 162
SmoI CTYRAG 1 cut(s) 162
Sse9I AATT 2 cut(s) 91, 296
SsiI CCGC 2 cut(s) 218, 357
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 259, 361
StyI CCWWGG 3 cut(s) 286, 317, 626
TasI AATT 2 cut(s) 91, 296
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 1 cut(s) 188
TseI GCWGC 2 cut(s) 251, 254
TspDTI ATGAA 5 cut(s) 263, 309, 537, 596, 624
TspGWI ACGGA 1 cut(s) 463
VpaK11BI GGWCC 2 cut(s) 263, 283
XapI RAATTY 1 cut(s) 91
XspI CTAG 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.