Rh2CG333400

zinc-RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0010929
Physical Location & Seq
Reverse (-)
43865086 .. 43867034
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG333400.1

Sequence Viewer

Length: 765 bp
ATGACGAGCAGAAGAACCGACTCTAACAGTTCAGCCACATTTAATCCGAGACTTGCCTACCCTCCTCCTCCTCCTCCTCCTCCTCGTACCCATGATAATCCATATGGCTACTATTTTTCTTCTCCTCCTCCACCTTCCCTTTTTTCTCCTCCTCCGCCCTCCCTTTCTTCTCCTCTTAACCATCTTACTCCATATGACTACTTTTTTTCAATTGATGATGATGATGTTAATGATCACTGGCATTTCGAGGAACCTGTTCAGCCCCTGTCAAATACCTTCCAAGTCACCCGAGTTACCAGAACAAGGAGGCCATTTGGGGGAACGATAGGAGTAGCACAACCACCGGGCTTTGCAGGCTCACTAGCACCCCCAGGTTCCCGAATTCATGCTTTGCTCCTCGACAATCCAAGGACTAGACAGTTCCTCAGTGGCCAATGGACTCCGGCAGTTCCTAGACAGGAAGCCGACTCCAGGTTGACCTATAATGAGCAAAAGAAGGCATTGCAGAAGCTCAGGAAGCAGATATACAATCCTTCATGGAGAATATCGCCAACGGTCAACTTGTATTATAGGGGCACTCCTGTTCAAGACAGTGGCATAGGAAGAAATGAGGGTAAGAGGTGTACCATTTGCTTGGAAGATTTTGAGCCTAAAGAAGAGGTTATGCTCACCCCATGTAACCATATGTTTCATGAGGAATGCATTGTGCCATGGGTCAAGAGCAACGGTCGGTGCCCGGTCTGTAGGTATGGGATAAGTGACTAA

Protein Analysis

254

Amino Acids

28.8

Weight (kDa)

8.51

Isoelectric Point (pI)

63.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 199 - 249 3.2e-10 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 205 - 252 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 207 - 249 1e-15 Ring finger domain
zf-C3HC4_2 PF13923 207 - 248 1.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 207 - 249 1.3e-08 zinc-RING finger domain
zf-RING_11 PF17123 208 - 235 3.2e-12 RING-like zinc finger
zf-C3HC4 PF00097 208 - 248 7.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 208 - 246 1.6e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58720 AT3G58720 AT3G58720 AT3G58720
fragaria_vesca FvH4_6g27790
malus_domestica MD09G1234400.v1.1 MD17G1263500.v1.1
prunus_persica Prupe.3G112600_v2.0.a1
pyrus_communis pycom09g15270 pycom17g26370
rosa_chinensis RchiOBHm_Chr2g0131091
rosa_laevigata RLG00000019166
rosa_multiflora Rmu_sc0003629.1_g000064
rosa_roxburghii Rroxscaffold_2G00113210
rosa_rugosa Rorug02G0294900
rosa_samantha Rh2AG347200 Rh2BG354700 Rh2CG333400 Rh2DG372600
rosa_wichuraiana Rw2G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 732
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 430
AcsI RAATTY 1 cut(s) 381
AfaI GTAC 2 cut(s) 88, 625
AfiI CCNNNNNNNGG 3 cut(s) 303, 317, 471
AgsI TTSAA 2 cut(s) 210, 587
AjnI CCWGG 2 cut(s) 370, 470
AluBI AGCT 1 cut(s) 511
AluI AGCT 1 cut(s) 511
Alw26I GTCTC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 2 cut(s) 308, 430
ApoI RAATTY 1 cut(s) 381
Asp700I GAANNNNTTC 1 cut(s) 255
AsuC2I CCSGG 2 cut(s) 345, 737
AsuHPI GGTGA 2 cut(s) 277, 661
AvaI CYCGRG 1 cut(s) 288
BaeGI GKGCMC 2 cut(s) 578, 737
BalI TGGCCA 1 cut(s) 432
BanI GGYRCC 1 cut(s) 732
BarI GAAGNNNNNNTAC 2 cut(s) 509, 541
BccI CCATC 1 cut(s) 189
BciT130I CCWGG 2 cut(s) 372, 472
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 2 cut(s) 345, 737
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 3 cut(s) 362, 414, 453
BfmI CTRYAG 1 cut(s) 742
Bme1390I CCNGG 4 cut(s) 345, 372, 472, 737
BmeT110I CYCGRG 1 cut(s) 288
BmiI GGNNCC 3 cut(s) 252, 376, 734
BmrFI CCNGG 4 cut(s) 345, 372, 472, 737
BpmI CTGGAG 1 cut(s) 454
Bpu10I CCTNAGC 1 cut(s) 512
BpuMI CCSGG 2 cut(s) 345, 737
BsaJI CCNNGG 3 cut(s) 370, 407, 710
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 317, 471
Bse1I ACTGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 500
BseBI CCWGG 2 cut(s) 372, 472
BseDI CCNNGG 3 cut(s) 370, 407, 710
BseLI CCNNNNNNNGG 3 cut(s) 303, 317, 471
BseMI GCAATG 1 cut(s) 500
BseMII CTCAG 2 cut(s) 439, 526
BseNI ACTGG 1 cut(s) 242
BseSI GKGCMC 2 cut(s) 578, 737
Bsh1285I CGRYCG 1 cut(s) 730
BshFI GGCC 2 cut(s) 310, 432
BshNI GGYRCC 1 cut(s) 732
BsiEI CGRYCG 1 cut(s) 730
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 3 cut(s) 344, 443, 737
BslI CCNNNNNNNGG 3 cut(s) 303, 317, 471
BsmAI GTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 2 cut(s) 310, 432
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 578, 737
Bsp143I GATC 1 cut(s) 232
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 1 cut(s) 155
BspANI GGCC 2 cut(s) 310, 432
BspCNI CTCAG 2 cut(s) 438, 525
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 3 cut(s) 252, 376, 734
BspT107I GGYRCC 1 cut(s) 732
BsrDI GCAATG 1 cut(s) 500
BsrI ACTGG 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 370, 407, 710
BssMI GATC 1 cut(s) 232
BssT1I CCWWGG 2 cut(s) 407, 710
Bst2UI CCWGG 2 cut(s) 372, 472
Bst4CI ACNGT 5 cut(s) 29, 420, 556, 593, 728
Bst6I CTCTTC 1 cut(s) 651
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 2 cut(s) 425, 512
BstDSI CCRYGG 1 cut(s) 710
BstKTI GATC 1 cut(s) 235
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 1 cut(s) 232
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 2 cut(s) 354, 517
BstNI CCWGG 2 cut(s) 372, 472
BstSCI CCNGG 4 cut(s) 343, 370, 470, 735
BstSFI CTRYAG 1 cut(s) 742
BstSLI GKGCMC 2 cut(s) 578, 737
BstXI CCANNNNNNTGG 1 cut(s) 634
BsuRI GGCC 2 cut(s) 310, 432
BtgI CCRYGG 1 cut(s) 710
BtsIMutI CAGTG 3 cut(s) 235, 433, 598
Cac8I GCNNGC 1 cut(s) 355
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 691
Csp6I GTAC 2 cut(s) 87, 624
CviAII CATG 6 cut(s) 92, 386, 537, 675, 692, 711
CviQI GTAC 2 cut(s) 87, 624
DdeI CTNAG 2 cut(s) 425, 512
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 430
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
EciI GGCGGA 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 407, 710
Eco88I CYCGRG 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 381
EcoRII CCWGG 2 cut(s) 370, 470
EcoT14I CCWWGG 2 cut(s) 407, 710
EcoT22I ATGCAT 1 cut(s) 704
ErhI CCWWGG 2 cut(s) 407, 710
FaeI CATG 6 cut(s) 95, 389, 540, 678, 695, 714
FatI CATG 6 cut(s) 91, 385, 536, 674, 691, 710
FauNDI CATATG 3 cut(s) 103, 193, 684
FbaI TGATCA 1 cut(s) 232
FspBI CTAG 3 cut(s) 362, 414, 453
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 2 cut(s) 310, 432
HapII CCGG 3 cut(s) 344, 443, 737
Hin1II CATG 6 cut(s) 95, 389, 540, 678, 695, 714
HincII GTYRAC 2 cut(s) 477, 559
HindII GTYRAC 2 cut(s) 477, 559
HinfI GANTC 3 cut(s) 20, 439, 467
HpaII CCGG 3 cut(s) 344, 443, 737
HphI GGTGA 2 cut(s) 277, 661
Hpy166II GTNNAC 3 cut(s) 477, 559, 624
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 5 cut(s) 378, 514, 587, 692, 718
Hpy8I GTNNAC 3 cut(s) 477, 559, 624
HpyAV CCTTC 4 cut(s) 144, 286, 490, 543
HpyCH4III ACNGT 5 cut(s) 29, 420, 556, 593, 728
HpyCH4V TGCA 3 cut(s) 353, 505, 702
HpyF10VI GCNNNNNNNGC 2 cut(s) 354, 517
HpyF3I CTNAG 2 cut(s) 425, 512
Hsp92II CATG 6 cut(s) 95, 389, 540, 678, 695, 714
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 399
MaeI CTAG 3 cut(s) 362, 414, 453
MaeIII GTNAC 4 cut(s) 283, 292, 677, 758
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 6 cut(s) 24, 111, 159, 615, 650, 668
MfeI CAATTG 1 cut(s) 210
MhlI GDGCHC 2 cut(s) 578, 737
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 2 cut(s) 210, 381
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 3 cut(s) 14, 433, 461
Mox20I TGGCCA 1 cut(s) 432
Mph1103I ATGCAT 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 255
MscI TGGCCA 1 cut(s) 432
MseI TTAA 3 cut(s) 42, 177, 228
Msp20I TGGCCA 1 cut(s) 432
MspI CCGG 3 cut(s) 344, 443, 737
MspR9I CCNGG 4 cut(s) 345, 372, 472, 737
MunI CAATTG 1 cut(s) 210
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 2 cut(s) 372, 472
MwoI GCNNNNNNNGC 2 cut(s) 354, 517
NciI CCSGG 2 cut(s) 345, 737
NcoI CCATGG 1 cut(s) 710
NdeI CATATG 3 cut(s) 103, 193, 684
NdeII GATC 1 cut(s) 232
NlaIII CATG 6 cut(s) 95, 389, 540, 678, 695, 714
NlaIV GGNNCC 3 cut(s) 252, 376, 734
NmuCI GTSAC 2 cut(s) 283, 758
NsiI ATGCAT 1 cut(s) 704
PagI TCATGA 1 cut(s) 691
PctI GAATGC 1 cut(s) 704
PdmI GAANNNNTTC 1 cut(s) 255
PleI GAGTC 3 cut(s) 14, 433, 461
PpsI GAGTC 3 cut(s) 14, 433, 461
Psp6I CCWGG 2 cut(s) 370, 470
PspGI CCWGG 2 cut(s) 370, 470
PspN4I GGNNCC 3 cut(s) 252, 376, 734
PstNI CAGNNNCTG 1 cut(s) 265
RsaI GTAC 2 cut(s) 88, 625
RsaNI GTAC 2 cut(s) 87, 624
SaqAI TTAA 3 cut(s) 42, 177, 228
Sau3AI GATC 1 cut(s) 232
SchI GAGTC 3 cut(s) 14, 433, 461
ScrFI CCNGG 4 cut(s) 345, 372, 472, 737
SduI GDGCHC 2 cut(s) 578, 737
SfcI CTRYAG 1 cut(s) 742
Sse9I AATT 2 cut(s) 210, 381
SsiI CCGC 1 cut(s) 155
SspMI CTAG 3 cut(s) 362, 414, 453
StyD4I CCNGG 4 cut(s) 343, 370, 470, 735
StyI CCWWGG 2 cut(s) 407, 710
TaaI ACNGT 5 cut(s) 29, 420, 556, 593, 728
TaqI TCGA 2 cut(s) 246, 399
TasI AATT 2 cut(s) 210, 381
Tru1I TTAA 3 cut(s) 42, 177, 228
Tru9I TTAA 3 cut(s) 42, 177, 228
TscAI CASTG 3 cut(s) 242, 433, 598
TseFI GTSAC 2 cut(s) 283, 758
Tsp45I GTSAC 2 cut(s) 283, 758
TspDTI ATGAA 3 cut(s) 374, 525, 680
TspRI CASTG 3 cut(s) 242, 433, 598
XapI RAATTY 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 3 cut(s) 362, 414, 453
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.