Rh2AG347200

zinc-RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010929
Physical Location & Seq
Reverse (-)
51066717 .. 51067761
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG347200.1

Sequence Viewer

Length: 519 bp
ATGAAGAGATCGTACATTTTTTTCGTAACATTAACTGATCACCAGGTTACCAGAACAAGGAGGCCATTTGGGGGAGCGATAGGAGTAGCACAACCACCGGGCTTTGCAGGCTCACTAGCACCACCAGGTTCCCGAATTCATGCTTTGCTCCTCGACAATCCAAGGACTAGACAGATCCTCAGCGGCCAATGGACTCTGGCAGCTCCTAGACAGGAAGCCGACTCCAGGTTGACCTATAATGAGCAAAAGAAGGCATTGCAGAAGCTCAGGAAGCAGATATACAATCCTTCACGGAGAATATCGCCAACGGTCAACTTGTATTATAGGGGCACTCCTGTTCAAGACAGTGGCATAGGAAGAAATGAGGGTAAGAGGTGTACCATTTGCTTGGAAGATTTTGAGCCTAAAGAAGAGGTTATGCTCACCCCATGTAACCATATGTTTCATGAGGAATGCATTGTGCCATGGGTCAAGAGCAACGGTCGGTGCCCGGTCTGTAGGTATGGGATAAGTGACTAA

Protein Analysis

172

Amino Acids

19.52

Weight (kDa)

9.62

Isoelectric Point (pI)

57.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 116 - 167 1.6e-10 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 123 - 170 5.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 125 - 167 5.4e-16 Ring finger domain
zf-C3HC4_2 PF13923 125 - 166 9.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 125 - 167 6.6e-09 zinc-RING finger domain
zf-RING_11 PF17123 126 - 153 1.8e-12 RING-like zinc finger
zf-C3HC4 PF00097 126 - 166 4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 126 - 164 1e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58720 AT3G58720 AT3G58720 AT3G58720
fragaria_vesca FvH4_6g27790
malus_domestica MD09G1234400.v1.1 MD17G1263500.v1.1
prunus_persica Prupe.3G112600_v2.0.a1
pyrus_communis pycom09g15270 pycom17g26370
rosa_chinensis RchiOBHm_Chr2g0131091
rosa_laevigata RLG00000019166
rosa_multiflora Rmu_sc0003629.1_g000064
rosa_roxburghii Rroxscaffold_2G00113210
rosa_rugosa Rorug02G0294900
rosa_samantha Rh2AG347200 Rh2BG354700 Rh2CG333400 Rh2DG372600
rosa_wichuraiana Rw2G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 486
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 1 cut(s) 135
AfaI GTAC 2 cut(s) 14, 379
AfiI CCNNNNNNNGG 3 cut(s) 57, 71, 225
AgsI TTSAA 1 cut(s) 341
AjnI CCWGG 3 cut(s) 42, 124, 224
AluBI AGCT 2 cut(s) 203, 265
AluI AGCT 2 cut(s) 203, 265
AlwI GGATC 1 cut(s) 169
AoxI GGCC 2 cut(s) 62, 184
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 135
AsuC2I CCSGG 2 cut(s) 99, 491
AsuHPI GGTGA 2 cut(s) 32, 415
BaeGI GKGCMC 2 cut(s) 332, 491
BanI GGYRCC 1 cut(s) 486
BarI GAAGNNNNNNTAC 3 cut(s) 28, 263, 295
BbvCI CCTCAGC 1 cut(s) 179
BbvI GCAGC 1 cut(s) 212
BciT130I CCWGG 3 cut(s) 44, 126, 226
BclI TGATCA 1 cut(s) 37
BcnI CCSGG 2 cut(s) 99, 491
BfaI CTAG 3 cut(s) 116, 168, 207
BfmI CTRYAG 1 cut(s) 496
BisI GCNGC 2 cut(s) 184, 201
BlsI GCNGC 2 cut(s) 185, 202
Bme1390I CCNGG 5 cut(s) 44, 99, 126, 226, 491
BmiI GGNNCC 2 cut(s) 130, 488
BmrFI CCNGG 5 cut(s) 44, 99, 126, 226, 491
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 2 cut(s) 179, 266
BpuMI CCSGG 2 cut(s) 99, 491
BsaJI CCNNGG 2 cut(s) 161, 464
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 71, 225
Bse3DI GCAATG 1 cut(s) 254
BseBI CCWGG 3 cut(s) 44, 126, 226
BseDI CCNNGG 2 cut(s) 161, 464
BseLI CCNNNNNNNGG 3 cut(s) 57, 71, 225
BseMI GCAATG 1 cut(s) 254
BseMII CTCAG 2 cut(s) 193, 280
BseRI GAGGAG 1 cut(s) 140
BseSI GKGCMC 2 cut(s) 332, 491
BseXI GCAGC 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 2 cut(s) 64, 186
BshNI GGYRCC 1 cut(s) 486
BsiEI CGRYCG 1 cut(s) 484
BsiSI CCGG 2 cut(s) 98, 491
BslI CCNNNNNNNGG 3 cut(s) 57, 71, 225
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 2 cut(s) 64, 186
Bsp1286I GDGCHC 2 cut(s) 332, 491
Bsp143I GATC 3 cut(s) 8, 37, 174
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 1 cut(s) 183
BspANI GGCC 2 cut(s) 64, 186
BspCNI CTCAG 2 cut(s) 192, 279
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 2 cut(s) 130, 488
BspPI GGATC 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 161, 464
BssMI GATC 3 cut(s) 8, 37, 174
BssT1I CCWWGG 2 cut(s) 161, 464
Bst2UI CCWGG 3 cut(s) 44, 126, 226
Bst4CI ACNGT 3 cut(s) 310, 347, 482
Bst6I CTCTTC 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 179, 266
BstDSI CCRYGG 1 cut(s) 464
BstEII GGTNACC 1 cut(s) 46
BstKTI GATC 3 cut(s) 11, 40, 177
BstMBI GATC 3 cut(s) 8, 37, 174
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 2 cut(s) 108, 271
BstNI CCWGG 3 cut(s) 44, 126, 226
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 5 cut(s) 42, 97, 124, 224, 489
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 2 cut(s) 332, 491
BstV1I GCAGC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 388
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 2 cut(s) 64, 186
BtgI CCRYGG 1 cut(s) 464
BtsIMutI CAGTG 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 109
CciI TCATGA 1 cut(s) 445
CsiI ACCWGGT 2 cut(s) 42, 124
Csp6I GTAC 2 cut(s) 13, 378
CviAII CATG 4 cut(s) 140, 429, 446, 465
CviJI RGCY 8 cut(s) 64, 102, 111, 186, 203, 218, 265, 403
CviKI_1 RGCY 8 cut(s) 64, 102, 111, 186, 203, 218, 265, 403
CviQI GTAC 2 cut(s) 13, 378
DdeI CTNAG 2 cut(s) 179, 266
DpnI GATC 3 cut(s) 10, 39, 176
DpnII GATC 3 cut(s) 8, 37, 174
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco130I CCWWGG 2 cut(s) 161, 464
Eco91I GGTNACC 1 cut(s) 46
EcoO65I GGTNACC 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 3 cut(s) 42, 124, 224
EcoT14I CCWWGG 2 cut(s) 161, 464
EcoT22I ATGCAT 1 cut(s) 458
ErhI CCWWGG 2 cut(s) 161, 464
FaeI CATG 4 cut(s) 143, 432, 449, 468
FatI CATG 4 cut(s) 139, 428, 445, 464
FauNDI CATATG 1 cut(s) 438
FbaI TGATCA 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 184, 201
Fsp4HI GCNGC 2 cut(s) 184, 201
FspBI CTAG 3 cut(s) 116, 168, 207
GluI GCNGC 2 cut(s) 184, 201
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 2 cut(s) 64, 186
HapII CCGG 2 cut(s) 98, 491
Hin1II CATG 4 cut(s) 143, 432, 449, 468
HincII GTYRAC 2 cut(s) 231, 313
HindII GTYRAC 2 cut(s) 231, 313
HinfI GANTC 2 cut(s) 193, 221
HpaII CCGG 2 cut(s) 98, 491
HphI GGTGA 2 cut(s) 32, 415
Hpy166II GTNNAC 3 cut(s) 231, 313, 378
Hpy188III TCNNGA 5 cut(s) 132, 268, 341, 446, 472
Hpy8I GTNNAC 3 cut(s) 231, 313, 378
HpyAV CCTTC 2 cut(s) 244, 297
HpyCH4III ACNGT 3 cut(s) 310, 347, 482
HpyCH4V TGCA 3 cut(s) 107, 259, 456
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 271
HpyF3I CTNAG 2 cut(s) 179, 266
Hsp92II CATG 4 cut(s) 143, 432, 449, 468
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 3 cut(s) 8, 37, 174
LmnI GCTCC 3 cut(s) 74, 153, 208
Lsp1109I GCAGC 1 cut(s) 212
MabI ACCWGGT 2 cut(s) 42, 124
MaeI CTAG 3 cut(s) 116, 168, 207
MaeIII GTNAC 4 cut(s) 25, 46, 431, 512
MalI GATC 3 cut(s) 10, 39, 176
MboI GATC 3 cut(s) 8, 37, 174
MboII GAAGA 4 cut(s) 16, 369, 404, 422
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 332, 491
MluCI AATT 1 cut(s) 135
MlyI GAGTC 2 cut(s) 187, 215
MnlI CCTC 7 cut(s) 54, 161, 188, 358, 366, 406, 442
Mph1103I ATGCAT 1 cut(s) 458
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 2 cut(s) 98, 491
MspR9I CCNGG 5 cut(s) 44, 99, 126, 226, 491
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 3 cut(s) 44, 126, 226
MwoI GCNNNNNNNGC 2 cut(s) 108, 271
NciI CCSGG 2 cut(s) 99, 491
NcoI CCATGG 1 cut(s) 464
NdeI CATATG 1 cut(s) 438
NdeII GATC 3 cut(s) 8, 37, 174
NlaIII CATG 4 cut(s) 143, 432, 449, 468
NlaIV GGNNCC 2 cut(s) 130, 488
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 458
PagI TCATGA 1 cut(s) 445
PctI GAATGC 1 cut(s) 458
PkrI GCNGC 2 cut(s) 185, 202
PleI GAGTC 2 cut(s) 187, 215
PpsI GAGTC 2 cut(s) 187, 215
Psp6I CCWGG 3 cut(s) 42, 124, 224
PspEI GGTNACC 1 cut(s) 46
PspGI CCWGG 3 cut(s) 42, 124, 224
PspN4I GGNNCC 2 cut(s) 130, 488
PsuI RGATCY 1 cut(s) 174
RsaI GTAC 2 cut(s) 14, 379
RsaNI GTAC 2 cut(s) 13, 378
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 2 cut(s) 184, 201
Sau3AI GATC 3 cut(s) 8, 37, 174
SchI GAGTC 2 cut(s) 187, 215
ScrFI CCNGG 5 cut(s) 44, 99, 126, 226, 491
SduI GDGCHC 2 cut(s) 332, 491
SetI ASST 9 cut(s) 48, 130, 205, 230, 236, 267, 377, 417, 503
SexAI ACCWGGT 2 cut(s) 42, 124
SfcI CTRYAG 1 cut(s) 496
Sse9I AATT 1 cut(s) 135
SsiI CCGC 1 cut(s) 183
SspMI CTAG 3 cut(s) 116, 168, 207
StyD4I CCNGG 5 cut(s) 42, 97, 124, 224, 489
StyI CCWWGG 2 cut(s) 161, 464
TaaI ACNGT 3 cut(s) 310, 347, 482
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 135
TauI GCSGC 1 cut(s) 186
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TscAI CASTG 1 cut(s) 352
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 3 cut(s) 17, 128, 434
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 1 cut(s) 352
XapI RAATTY 1 cut(s) 135
XspI CTAG 3 cut(s) 116, 168, 207
Zsp2I ATGCAT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.