RLG00000019166

zinc-RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010929
Physical Location & Seq
Reverse (-)
47609946 .. 47611415
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019166

Sequence Viewer

Length: 786 bp
ATGACGAGCAGAAGAACCAACTCTAACAGTTCAGCCACATTTAATCCGAGACTTGCCTACCCTCCTCCTTCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCGTACCCATGATAATCCATATGACCACTTTTTTCCTTCTTCTTCTCCACCTTCCCTTTCTTCTCCTCCTCCGCCCTCTCTTTCTTCTCCTCTTAACCATCTTACTCCATATGACTACTTTTTTTCAATTGATGACGATGATGTTAATGATCACTGGCATTTCGAGGAACCTGTTCAGCCCCTGTCAAATACCTTCCAAGTCACCCGAGTTACCAGAACAAGGAGGCCATTTGGGGGAGCGATAGGAGTAGCACAACCACCGGGCTTTGCAGGCTCACTAGCACCACCAGGTTCCCGAATTCATGCTTTGCTCCTCGACAATCCAAGGACTAGACAGATACTCAGCGGTCAATGGACTCTGGCAGCTCCTAGACAGGAAGCCGACTCCAGGTTGACCTATAATGAGCAAAAGAAGGCATTGCAGAAGCTCAGGAAGCAGATATACAATCCTTCAAGAAGAATATCGCCAACGGTCAACTTGTATTATAGAGGCACTCCTGTTCAAGACAGTGGCATAGGAAGAAATGAGGGTAAGAGGTGTACCATTTGCTTGGAAGATTTTGAGCCTAAAGAAGAGGTTATGCTCACCCCATGTAACCATATGTTTCATGAGGAATGCATTGTGCCATGGGTCAAGAGCAACGGTCGGTGCCCGGTCTGTAGGTATGGGATAAGTGACTAA

Protein Analysis

262

Amino Acids

29.32

Weight (kDa)

8.75

Isoelectric Point (pI)

70.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 206 - 256 3.3e-10 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 212 - 259 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 214 - 256 1.1e-15 Ring finger domain
zf-C3HC4_2 PF13923 214 - 255 1.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 214 - 256 1.3e-08 zinc-RING finger domain
zf-RING_11 PF17123 215 - 242 3.3e-12 RING-like zinc finger
zf-C3HC4 PF00097 215 - 255 7.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 215 - 253 1.5e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58720 AT3G58720 AT3G58720 AT3G58720
fragaria_vesca FvH4_6g27790
malus_domestica MD09G1234400.v1.1 MD17G1263500.v1.1
prunus_persica Prupe.3G112600_v2.0.a1
pyrus_communis pycom09g15270 pycom17g26370
rosa_chinensis RchiOBHm_Chr2g0131091
rosa_laevigata RLG00000019166
rosa_multiflora Rmu_sc0003629.1_g000064
rosa_roxburghii Rroxscaffold_2G00113210
rosa_rugosa Rorug02G0294900
rosa_samantha Rh2AG347200 Rh2BG354700 Rh2CG333400 Rh2DG372600
rosa_wichuraiana Rw2G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 753
AciI CCGC 2 cut(s) 176, 450
AcsI RAATTY 1 cut(s) 402
AfaI GTAC 2 cut(s) 109, 646
AfiI CCNNNNNNNGG 3 cut(s) 324, 338, 492
AgsI TTSAA 3 cut(s) 231, 558, 608
AjnI CCWGG 2 cut(s) 391, 491
AluBI AGCT 2 cut(s) 470, 532
AluI AGCT 2 cut(s) 470, 532
Alw26I GTCTC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 286
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 1 cut(s) 329
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 276
AsuC2I CCSGG 2 cut(s) 366, 758
AsuHPI GGTGA 2 cut(s) 298, 682
AvaI CYCGRG 1 cut(s) 309
BaeGI GKGCMC 1 cut(s) 758
BanI GGYRCC 1 cut(s) 753
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BbvI GCAGC 1 cut(s) 479
BccI CCATC 1 cut(s) 210
BciT130I CCWGG 2 cut(s) 393, 493
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 2 cut(s) 366, 758
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 3 cut(s) 383, 435, 474
BfmI CTRYAG 1 cut(s) 763
BisI GCNGC 1 cut(s) 468
BlsI GCNGC 1 cut(s) 469
Bme1390I CCNGG 4 cut(s) 366, 393, 493, 758
BmeT110I CYCGRG 1 cut(s) 309
BmiI GGNNCC 3 cut(s) 273, 397, 755
BmrFI CCNGG 4 cut(s) 366, 393, 493, 758
BpmI CTGGAG 1 cut(s) 475
Bpu10I CCTNAGC 1 cut(s) 533
BpuMI CCSGG 2 cut(s) 366, 758
BsaJI CCNNGG 2 cut(s) 428, 731
Bsc4I CCNNNNNNNGG 3 cut(s) 324, 338, 492
Bse1I ACTGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 521
BseBI CCWGG 2 cut(s) 393, 493
BseDI CCNNGG 2 cut(s) 428, 731
BseLI CCNNNNNNNGG 3 cut(s) 324, 338, 492
BseMI GCAATG 1 cut(s) 521
BseMII CTCAG 2 cut(s) 460, 547
BseNI ACTGG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 758
BseXI GCAGC 1 cut(s) 479
Bsh1285I CGRYCG 1 cut(s) 751
BshFI GGCC 1 cut(s) 331
BshNI GGYRCC 1 cut(s) 753
BsiEI CGRYCG 1 cut(s) 751
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 365, 758
BslI CCNNNNNNNGG 3 cut(s) 324, 338, 492
BsmAI GTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 1 cut(s) 331
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 758
Bsp143I GATC 1 cut(s) 253
Bsp19I CCATGG 1 cut(s) 731
BspACI CCGC 2 cut(s) 176, 450
BspANI GGCC 1 cut(s) 331
BspCNI CTCAG 2 cut(s) 459, 546
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 273, 397, 755
BspT107I GGYRCC 1 cut(s) 753
BsrDI GCAATG 1 cut(s) 521
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 428, 731
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 2 cut(s) 428, 731
Bst2UI CCWGG 2 cut(s) 393, 493
Bst4CI ACNGT 4 cut(s) 29, 577, 614, 749
Bst6I CTCTTC 2 cut(s) 78, 672
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 2 cut(s) 446, 533
BstDSI CCRYGG 1 cut(s) 731
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 1 cut(s) 253
BstMCI CGRYCG 1 cut(s) 751
BstMWI GCNNNNNNNGC 2 cut(s) 375, 538
BstNI CCWGG 2 cut(s) 393, 493
BstSCI CCNGG 4 cut(s) 364, 391, 491, 756
BstSFI CTRYAG 1 cut(s) 763
BstSLI GKGCMC 1 cut(s) 758
BstV1I GCAGC 1 cut(s) 479
BstXI CCANNNNNNTGG 1 cut(s) 655
BsuRI GGCC 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 731
BtsIMutI CAGTG 2 cut(s) 256, 619
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 286
CciI TCATGA 1 cut(s) 712
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 2 cut(s) 108, 645
CviAII CATG 5 cut(s) 113, 407, 696, 713, 732
CviJI RGCY 9 cut(s) 35, 283, 331, 369, 378, 470, 485, 532, 670
CviKI_1 RGCY 9 cut(s) 35, 283, 331, 369, 378, 470, 485, 532, 670
CviQI GTAC 2 cut(s) 108, 645
DdeI CTNAG 2 cut(s) 446, 533
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 78, 672
EarI CTCTTC 2 cut(s) 78, 672
EciI GGCGGA 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 428, 731
Eco88I CYCGRG 1 cut(s) 309
EcoRI GAATTC 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 391, 491
EcoT14I CCWWGG 2 cut(s) 428, 731
EcoT22I ATGCAT 1 cut(s) 725
ErhI CCWWGG 2 cut(s) 428, 731
FaeI CATG 5 cut(s) 116, 410, 699, 716, 735
FatI CATG 5 cut(s) 112, 406, 695, 712, 731
FauNDI CATATG 3 cut(s) 124, 214, 705
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 468
Fsp4HI GCNGC 1 cut(s) 468
FspBI CTAG 3 cut(s) 383, 435, 474
GluI GCNGC 1 cut(s) 468
GsuI CTGGAG 1 cut(s) 475
HaeIII GGCC 1 cut(s) 331
HapII CCGG 2 cut(s) 365, 758
Hin1II CATG 5 cut(s) 116, 410, 699, 716, 735
HincII GTYRAC 2 cut(s) 498, 580
HindII GTYRAC 2 cut(s) 498, 580
HinfI GANTC 2 cut(s) 460, 488
HpaII CCGG 2 cut(s) 365, 758
HphI GGTGA 2 cut(s) 298, 682
Hpy166II GTNNAC 3 cut(s) 498, 580, 645
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 6 cut(s) 399, 535, 558, 608, 713, 739
Hpy8I GTNNAC 3 cut(s) 498, 580, 645
HpyAV CCTTC 6 cut(s) 78, 150, 165, 307, 511, 564
HpyCH4III ACNGT 4 cut(s) 29, 577, 614, 749
HpyCH4V TGCA 3 cut(s) 374, 526, 723
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 538
HpyF3I CTNAG 2 cut(s) 446, 533
Hsp92II CATG 5 cut(s) 116, 410, 699, 716, 735
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 1 cut(s) 253
LmnI GCTCC 3 cut(s) 341, 420, 475
Lsp1109I GCAGC 1 cut(s) 479
MabI ACCWGGT 1 cut(s) 391
MaeI CTAG 3 cut(s) 383, 435, 474
MaeIII GTNAC 4 cut(s) 304, 313, 698, 779
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MfeI CAATTG 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 758
MluCI AATT 2 cut(s) 231, 402
MlyI GAGTC 2 cut(s) 454, 482
Mph1103I ATGCAT 1 cut(s) 725
MroXI GAANNNNTTC 1 cut(s) 276
MseI TTAA 3 cut(s) 42, 198, 249
MspA1I CMGCKG 1 cut(s) 450
MspI CCGG 2 cut(s) 365, 758
MspR9I CCNGG 4 cut(s) 366, 393, 493, 758
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 2 cut(s) 393, 493
MwoI GCNNNNNNNGC 2 cut(s) 375, 538
NciI CCSGG 2 cut(s) 366, 758
NcoI CCATGG 1 cut(s) 731
NdeI CATATG 3 cut(s) 124, 214, 705
NdeII GATC 1 cut(s) 253
NlaIII CATG 5 cut(s) 116, 410, 699, 716, 735
NlaIV GGNNCC 3 cut(s) 273, 397, 755
NmuCI GTSAC 2 cut(s) 304, 779
NsiI ATGCAT 1 cut(s) 725
PagI TCATGA 1 cut(s) 712
PctI GAATGC 1 cut(s) 725
PdmI GAANNNNTTC 1 cut(s) 276
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 2 cut(s) 454, 482
PpsI GAGTC 2 cut(s) 454, 482
Psp6I CCWGG 2 cut(s) 391, 491
PspGI CCWGG 2 cut(s) 391, 491
PspN4I GGNNCC 3 cut(s) 273, 397, 755
PstNI CAGNNNCTG 1 cut(s) 286
RsaI GTAC 2 cut(s) 109, 646
RsaNI GTAC 2 cut(s) 108, 645
SaqAI TTAA 3 cut(s) 42, 198, 249
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 1 cut(s) 253
SchI GAGTC 2 cut(s) 454, 482
ScrFI CCNGG 4 cut(s) 366, 393, 493, 758
SduI GDGCHC 1 cut(s) 758
SexAI ACCWGGT 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 763
Sse9I AATT 2 cut(s) 231, 402
SsiI CCGC 2 cut(s) 176, 450
SspMI CTAG 3 cut(s) 383, 435, 474
StyD4I CCNGG 4 cut(s) 364, 391, 491, 756
StyI CCWWGG 2 cut(s) 428, 731
TaaI ACNGT 4 cut(s) 29, 577, 614, 749
TaqI TCGA 2 cut(s) 267, 420
TasI AATT 2 cut(s) 231, 402
Tru1I TTAA 3 cut(s) 42, 198, 249
Tru9I TTAA 3 cut(s) 42, 198, 249
TscAI CASTG 2 cut(s) 263, 619
TseFI GTSAC 2 cut(s) 304, 779
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 2 cut(s) 304, 779
TspDTI ATGAA 2 cut(s) 395, 701
TspRI CASTG 2 cut(s) 263, 619
XapI RAATTY 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 276
XspI CTAG 3 cut(s) 383, 435, 474
Zsp2I ATGCAT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.