Rh2BG354700

zinc-RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010929
Physical Location & Seq
Reverse (-)
48727226 .. 48729162
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG354700.1

Sequence Viewer

Length: 780 bp
ATGATGAACAGAAGAACCAACTCTAACAGTTCAGCCACATTTAATCCGAGACTTGCCTACCCTCCTCCTTCTCTTCCTCCTCCTCCTCCGCCTCCTCCTCGTACCCATGATAATCCATATGACTACTTTTTTCCATCTTCTTCTCCACCTTCCCTTTCTTCTCCTCCTCCGCCCTCCCTTTCTTCTCCTCTTAACCATCTTACTCCATATGACTACTTTTTTTCAATTGATGATGATGATGTTAATGATCACTGGCATTTCGAGGAACCTGTTCAGCCCCTGTCAAATACCTTCCAAGTCACCCGAGTTACCAGAACAAGGAGGCCATTTGGGGGAGCGATAGGAGTAGCACAACCACCGGGCTTTGCAGGCTCACTAGCACCACCAGGTTCCCGAATTCATGCTTTGCTCCTCGACAATCCAAGGACTAGACAGATCATCAGCGGCCAATGGACTCTGGCAGCTCCTAGACAGGAAGCCGACTCCAGGTTGACCTATAATGAGCAAAAGAAGGCATTGCAGAAGCTCAGGAAGCAGATATACAATCCTTCACGGAGAATATCGCCAACGGTCAACTTGTATTATAGAGGTACTCCTGTTCAAGACAGTGGCATAGGAAGAAATGAGGGTAAGAGGTGTACCATTTGCTTGGAAGATTTTGAGCCTAAAGAAGAGGTTATGCTCACCCCATGTAACCATATGTTTCATGAGGAATGCATTGTGCCATGGGTCAAGAGCAACGGTCGGTGCCCGGTCTGTAGGTATGGGATAAGTGACTAA

Protein Analysis

259

Amino Acids

29.21

Weight (kDa)

8.74

Isoelectric Point (pI)

68.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 204 - 254 3.3e-10 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 210 - 257 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 212 - 254 1.1e-15 Ring finger domain
zf-C3HC4_2 PF13923 212 - 253 1.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 212 - 254 1.3e-08 zinc-RING finger domain
zf-RING_11 PF17123 213 - 240 3.2e-12 RING-like zinc finger
zf-C3HC4 PF00097 213 - 253 7.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 213 - 251 1.6e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010929)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58720 AT3G58720 AT3G58720 AT3G58720
fragaria_vesca FvH4_6g27790
malus_domestica MD09G1234400.v1.1 MD17G1263500.v1.1
prunus_persica Prupe.3G112600_v2.0.a1
pyrus_communis pycom09g15270 pycom17g26370
rosa_chinensis RchiOBHm_Chr2g0131091
rosa_laevigata RLG00000019166
rosa_multiflora Rmu_sc0003629.1_g000064
rosa_roxburghii Rroxscaffold_2G00113210
rosa_rugosa Rorug02G0294900
rosa_samantha Rh2AG347200 Rh2BG354700 Rh2CG333400 Rh2DG372600
rosa_wichuraiana Rw2G028190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 747
AciI CCGC 3 cut(s) 89, 170, 444
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 1 cut(s) 396
AfaI GTAC 3 cut(s) 103, 592, 640
AfiI CCNNNNNNNGG 3 cut(s) 318, 332, 486
AgsI TTSAA 2 cut(s) 225, 602
AjnI CCWGG 2 cut(s) 385, 485
AluBI AGCT 2 cut(s) 464, 526
AluI AGCT 2 cut(s) 464, 526
Alw26I GTCTC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 280
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 2 cut(s) 323, 445
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 270
AsuC2I CCSGG 2 cut(s) 360, 752
AsuHPI GGTGA 2 cut(s) 292, 676
AvaI CYCGRG 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 752
BaeI ACNNNNGTAYC 2 cut(s) 582, 615
BanI GGYRCC 1 cut(s) 747
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
BbvI GCAGC 1 cut(s) 473
BccI CCATC 2 cut(s) 142, 204
BciT130I CCWGG 2 cut(s) 387, 487
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 2 cut(s) 360, 752
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 3 cut(s) 377, 429, 468
BfmI CTRYAG 1 cut(s) 757
BisI GCNGC 2 cut(s) 445, 462
BlsI GCNGC 2 cut(s) 446, 463
Bme1390I CCNGG 4 cut(s) 360, 387, 487, 752
BmeT110I CYCGRG 1 cut(s) 303
BmiI GGNNCC 3 cut(s) 267, 391, 749
BmrFI CCNGG 4 cut(s) 360, 387, 487, 752
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 1 cut(s) 527
BpuMI CCSGG 2 cut(s) 360, 752
BsaJI CCNNGG 2 cut(s) 422, 725
Bsc4I CCNNNNNNNGG 3 cut(s) 318, 332, 486
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 515
BseBI CCWGG 2 cut(s) 387, 487
BseDI CCNNGG 2 cut(s) 422, 725
BseLI CCNNNNNNNGG 3 cut(s) 318, 332, 486
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 1 cut(s) 541
BseNI ACTGG 1 cut(s) 257
BseSI GKGCMC 1 cut(s) 752
BseXI GCAGC 1 cut(s) 473
Bsh1285I CGRYCG 1 cut(s) 745
BshFI GGCC 2 cut(s) 325, 447
BshNI GGYRCC 1 cut(s) 747
BsiEI CGRYCG 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 2 cut(s) 359, 752
BslI CCNNNNNNNGG 3 cut(s) 318, 332, 486
BsmAI GTCTC 1 cut(s) 43
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 2 cut(s) 325, 447
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 752
Bsp143I GATC 2 cut(s) 247, 435
Bsp19I CCATGG 1 cut(s) 725
BspACI CCGC 3 cut(s) 89, 170, 444
BspANI GGCC 2 cut(s) 325, 447
BspCNI CTCAG 1 cut(s) 540
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 3 cut(s) 267, 391, 749
BspT107I GGYRCC 1 cut(s) 747
BsrDI GCAATG 1 cut(s) 515
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 422, 725
BssMI GATC 2 cut(s) 247, 435
BssT1I CCWWGG 2 cut(s) 422, 725
Bst2UI CCWGG 2 cut(s) 387, 487
Bst4CI ACNGT 4 cut(s) 29, 571, 608, 743
Bst6I CTCTTC 2 cut(s) 78, 666
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 1 cut(s) 527
BstDSI CCRYGG 1 cut(s) 725
BstKTI GATC 2 cut(s) 250, 438
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 2 cut(s) 247, 435
BstMCI CGRYCG 1 cut(s) 745
BstMWI GCNNNNNNNGC 2 cut(s) 369, 532
BstNI CCWGG 2 cut(s) 387, 487
BstSCI CCNGG 4 cut(s) 358, 385, 485, 750
BstSFI CTRYAG 1 cut(s) 757
BstSLI GKGCMC 1 cut(s) 752
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 649
BsuRI GGCC 2 cut(s) 325, 447
BtgI CCRYGG 1 cut(s) 725
BtsIMutI CAGTG 2 cut(s) 250, 613
Cac8I GCNNGC 1 cut(s) 370
CaiI CAGNNNCTG 1 cut(s) 280
CciI TCATGA 1 cut(s) 706
CsiI ACCWGGT 1 cut(s) 385
Csp6I GTAC 3 cut(s) 102, 591, 639
CviAII CATG 5 cut(s) 107, 401, 690, 707, 726
CviQI GTAC 3 cut(s) 102, 591, 639
DdeI CTNAG 1 cut(s) 527
DpnI GATC 2 cut(s) 249, 437
DpnII GATC 2 cut(s) 247, 435
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 2 cut(s) 78, 666
EarI CTCTTC 2 cut(s) 78, 666
EciI GGCGGA 2 cut(s) 78, 159
Eco130I CCWWGG 2 cut(s) 422, 725
Eco88I CYCGRG 1 cut(s) 303
EcoRI GAATTC 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 385, 485
EcoT14I CCWWGG 2 cut(s) 422, 725
EcoT22I ATGCAT 1 cut(s) 719
ErhI CCWWGG 2 cut(s) 422, 725
FaeI CATG 5 cut(s) 110, 404, 693, 710, 729
FatI CATG 5 cut(s) 106, 400, 689, 706, 725
FauNDI CATATG 3 cut(s) 118, 208, 699
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 2 cut(s) 445, 462
Fsp4HI GCNGC 2 cut(s) 445, 462
FspBI CTAG 3 cut(s) 377, 429, 468
GluI GCNGC 2 cut(s) 445, 462
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 2 cut(s) 325, 447
HapII CCGG 2 cut(s) 359, 752
Hin1II CATG 5 cut(s) 110, 404, 693, 710, 729
HincII GTYRAC 2 cut(s) 492, 574
HindII GTYRAC 2 cut(s) 492, 574
HinfI GANTC 2 cut(s) 454, 482
HpaII CCGG 2 cut(s) 359, 752
HphI GGTGA 2 cut(s) 292, 676
Hpy166II GTNNAC 3 cut(s) 492, 574, 639
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 5 cut(s) 393, 529, 602, 707, 733
Hpy8I GTNNAC 3 cut(s) 492, 574, 639
HpyAV CCTTC 5 cut(s) 78, 159, 301, 505, 558
HpyCH4III ACNGT 4 cut(s) 29, 571, 608, 743
HpyCH4V TGCA 3 cut(s) 368, 520, 717
HpyF10VI GCNNNNNNNGC 2 cut(s) 369, 532
HpyF3I CTNAG 1 cut(s) 527
Hsp92II CATG 5 cut(s) 110, 404, 693, 710, 729
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 247, 435
LmnI GCTCC 3 cut(s) 335, 414, 469
Lsp1109I GCAGC 1 cut(s) 473
MabI ACCWGGT 1 cut(s) 385
MaeI CTAG 3 cut(s) 377, 429, 468
MaeIII GTNAC 4 cut(s) 298, 307, 692, 773
MalI GATC 2 cut(s) 249, 437
MboI GATC 2 cut(s) 247, 435
MboII GAAGA 9 cut(s) 24, 65, 129, 132, 150, 174, 630, 665, 683
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 752
MluCI AATT 2 cut(s) 225, 396
MlyI GAGTC 2 cut(s) 448, 476
Mph1103I ATGCAT 1 cut(s) 719
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 3 cut(s) 42, 192, 243
MspA1I CMGCKG 1 cut(s) 444
MspI CCGG 2 cut(s) 359, 752
MspR9I CCNGG 4 cut(s) 360, 387, 487, 752
MunI CAATTG 1 cut(s) 225
Mva1269I GAATGC 1 cut(s) 719
MvaI CCWGG 2 cut(s) 387, 487
MwoI GCNNNNNNNGC 2 cut(s) 369, 532
NciI CCSGG 2 cut(s) 360, 752
NcoI CCATGG 1 cut(s) 725
NdeI CATATG 3 cut(s) 118, 208, 699
NdeII GATC 2 cut(s) 247, 435
NlaIII CATG 5 cut(s) 110, 404, 693, 710, 729
NlaIV GGNNCC 3 cut(s) 267, 391, 749
NmuCI GTSAC 2 cut(s) 298, 773
NsiI ATGCAT 1 cut(s) 719
PagI TCATGA 1 cut(s) 706
PctI GAATGC 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 270
PkrI GCNGC 2 cut(s) 446, 463
PleI GAGTC 2 cut(s) 448, 476
PpsI GAGTC 2 cut(s) 448, 476
Psp6I CCWGG 2 cut(s) 385, 485
PspGI CCWGG 2 cut(s) 385, 485
PspN4I GGNNCC 3 cut(s) 267, 391, 749
PstNI CAGNNNCTG 1 cut(s) 280
RsaI GTAC 3 cut(s) 103, 592, 640
RsaNI GTAC 3 cut(s) 102, 591, 639
SaqAI TTAA 3 cut(s) 42, 192, 243
SatI GCNGC 2 cut(s) 445, 462
Sau3AI GATC 2 cut(s) 247, 435
SchI GAGTC 2 cut(s) 448, 476
ScrFI CCNGG 4 cut(s) 360, 387, 487, 752
SduI GDGCHC 1 cut(s) 752
SexAI ACCWGGT 1 cut(s) 385
SfcI CTRYAG 1 cut(s) 757
Sse9I AATT 2 cut(s) 225, 396
SsiI CCGC 3 cut(s) 89, 170, 444
SspMI CTAG 3 cut(s) 377, 429, 468
StyD4I CCNGG 4 cut(s) 358, 385, 485, 750
StyI CCWWGG 2 cut(s) 422, 725
TaaI ACNGT 4 cut(s) 29, 571, 608, 743
TaqI TCGA 2 cut(s) 261, 414
TasI AATT 2 cut(s) 225, 396
TauI GCSGC 1 cut(s) 447
Tru1I TTAA 3 cut(s) 42, 192, 243
Tru9I TTAA 3 cut(s) 42, 192, 243
TscAI CASTG 2 cut(s) 257, 613
TseFI GTSAC 2 cut(s) 298, 773
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 2 cut(s) 298, 773
TspDTI ATGAA 3 cut(s) 20, 389, 695
TspGWI ACGGA 1 cut(s) 568
TspRI CASTG 2 cut(s) 257, 613
XapI RAATTY 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 3 cut(s) 377, 429, 468
Zsp2I ATGCAT 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.