MD17G1279300.v1.1

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004371
Physical Location & Seq
Forward (+)
33940456 .. 33941854
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1279300.v1.1.491

Sequence Viewer

Length: 501 bp
ATGTCTTGTACTTCTGCAACTGCTGTAGCATCAGCCTACATTTCCAGGCCCAACTGGCCCGGCAGTCTCACCCACATACCCATCTGGAGAAGCTCTTCTCTCAGACCGCCGCCGCCATCTTCTCTCTGTGACTTCTCCTTCAAGATCCCATGTGGGTCTTTCAGGCGGAGCTCCGTCCTCACCGGATTCAGAAGTACGTCCCCCGTCGTTATGCAATGGCAGGACTGCACAGTAAAGATGGAAATTGATGTGCCTATTTCAGTTGCCTATGATTGTTACTCTGACCGTGAGGCGATTCCCCGTTGGATGCCATTTATTTCAACCGTGAAGATATTGGAAGACAAACCTGACTTATCACGATGGTCACTGAAGTATAAAGCTTTTGGTCGTAATATTGAATTATCATGGCTTGCTCGGAATATGCAGCCTACACCAAATCAGAAAATCCACTGGAGATCTTTGGAAGGTCTTCCTAACAGGTCAGAAAGCAAGAAATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc

Protein Analysis

167

Amino Acids

18.97

Weight (kDa)

9.63

Isoelectric Point (pI)

82.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 82 - 157 1.3e-10 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 107, 110, 113, 166
AclWI GGATC 1 cut(s) 139
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 389
AfaI GTAC 2 cut(s) 10, 196
AgsI TTSAA 3 cut(s) 142, 321, 398
AjnI CCWGG 1 cut(s) 44
AluBI AGCT 3 cut(s) 93, 171, 380
AluI AGCT 3 cut(s) 93, 171, 380
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 71
AlwI GGATC 1 cut(s) 139
AoxI GGCC 2 cut(s) 47, 56
ApeKI GCWGC 1 cut(s) 424
ApoI RAATTY 1 cut(s) 494
Asp700I GAANNNNTTC 2 cut(s) 94, 468
AspS9I GGNCC 2 cut(s) 48, 57
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 61, 172
BanII GRGCYC 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 345, 461
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 1 cut(s) 436
BccI CCATC 4 cut(s) 89, 124, 232, 354
BciT130I CCWGG 1 cut(s) 46
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 55
BglII AGATCT 1 cut(s) 455
BisI GCNGC 3 cut(s) 110, 113, 425
BlsI GCNGC 3 cut(s) 111, 114, 426
Bme1390I CCNGG 2 cut(s) 46, 60
BmgT120I GGNCC 2 cut(s) 48, 57
BmrFI CCNGG 2 cut(s) 46, 60
BmsI GCATC 2 cut(s) 38, 297
BpiI GAAGAC 2 cut(s) 345, 461
BpmI CTGGAG 2 cut(s) 106, 472
BpuMI CCSGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 59, 455
Bse3DI GCAATG 1 cut(s) 221
BseBI CCWGG 1 cut(s) 46
BseGI GGATG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 221
BseMII CTCAG 1 cut(s) 115
BseNI ACTGG 2 cut(s) 59, 455
BseXI GCAGC 1 cut(s) 436
BsgI GTGCAG 1 cut(s) 211
BshFI GGCC 2 cut(s) 49, 58
BsiHKAI GWGCWC 1 cut(s) 173
BsiSI CCGG 2 cut(s) 60, 183
BslFI GGGAC 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 2 cut(s) 49, 58
Bsp1286I GDGCHC 1 cut(s) 173
Bsp143I GATC 2 cut(s) 144, 455
BspACI CCGC 4 cut(s) 107, 110, 113, 166
BspANI GGCC 2 cut(s) 49, 58
BspCNI CTCAG 1 cut(s) 114
BspPI GGATC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 2 cut(s) 59, 455
BssMI GATC 2 cut(s) 144, 455
Bst2UI CCWGG 1 cut(s) 46
Bst4CI ACNGT 3 cut(s) 232, 287, 325
Bst6I CTCTTC 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 312
BstKTI GATC 2 cut(s) 147, 458
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 2 cut(s) 144, 455
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 44, 58
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 436
BstV2I GAAGAC 2 cut(s) 345, 461
BstX2I RGATCY 2 cut(s) 144, 455
BstYI RGATCY 2 cut(s) 144, 455
BsuRI GGCC 2 cut(s) 49, 58
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 365, 448
Cac8I GCNNGC 1 cut(s) 411
Cfr13I GGNCC 2 cut(s) 48, 57
Csp6I GTAC 2 cut(s) 9, 195
CviAII CATG 2 cut(s) 150, 405
CviJI RGCY 8 cut(s) 35, 49, 58, 93, 171, 380, 409, 427
CviKI_1 RGCY 8 cut(s) 35, 49, 58, 93, 171, 380, 409, 427
CviQI GTAC 2 cut(s) 9, 195
DdeI CTNAG 1 cut(s) 101
DpnI GATC 2 cut(s) 146, 457
DpnII GATC 2 cut(s) 144, 455
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 389
EcoICRI GAGCTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 44
EcoT38I GRGCYC 1 cut(s) 173
FaeI CATG 2 cut(s) 153, 408
FaiI YATR 7 cut(s) 77, 151, 212, 270, 375, 406, 422
FaqI GGGAC 1 cut(s) 184
FatI CATG 2 cut(s) 149, 404
Fnu4HI GCNGC 3 cut(s) 110, 113, 425
FokI GGATG 1 cut(s) 319
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 3 cut(s) 110, 113, 425
GluI GCNGC 3 cut(s) 110, 113, 425
GsuI CTGGAG 2 cut(s) 106, 472
HaeIII GGCC 2 cut(s) 49, 58
HapII CCGG 2 cut(s) 60, 183
Hin1II CATG 2 cut(s) 153, 408
HindIII AAGCTT 1 cut(s) 378
HinfI GANTC 2 cut(s) 186, 295
HpaII CCGG 2 cut(s) 60, 183
HphI GGTGA 2 cut(s) 61, 172
Hpy188I TCNGA 6 cut(s) 104, 191, 283, 417, 441, 484
Hpy188III TCNNGA 3 cut(s) 85, 142, 357
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 148, 458
HpyCH4III ACNGT 3 cut(s) 232, 287, 325
HpyCH4IV ACGT 1 cut(s) 197
HpyCH4V TGCA 4 cut(s) 17, 214, 228, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 1 cut(s) 197
Hsp92II CATG 2 cut(s) 153, 408
Kzo9I GATC 2 cut(s) 144, 455
LguI GCTCTTC 1 cut(s) 100
LmnI GCTCC 2 cut(s) 168, 176
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 2 cut(s) 38, 297
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 3 cut(s) 128, 275, 363
MalI GATC 2 cut(s) 146, 457
MboI GATC 2 cut(s) 144, 455
MboII GAAGA 5 cut(s) 87, 111, 340, 350, 461
MflI RGATCY 2 cut(s) 144, 455
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 3 cut(s) 243, 398, 494
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 2 cut(s) 188, 283
MroXI GAANNNNTTC 2 cut(s) 94, 468
MspI CCGG 2 cut(s) 60, 183
MspR9I CCNGG 2 cut(s) 46, 60
MvaI CCWGG 1 cut(s) 46
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 60
NdeII GATC 2 cut(s) 144, 455
NlaIII CATG 2 cut(s) 153, 408
NmuCI GTSAC 2 cut(s) 128, 363
PciSI GCTCTTC 1 cut(s) 100
PdmI GAANNNNTTC 2 cut(s) 94, 468
PfeI GAWTC 2 cut(s) 186, 295
PkrI GCNGC 3 cut(s) 111, 114, 426
Psp124BI GAGCTC 1 cut(s) 173
Psp6I CCWGG 1 cut(s) 44
PspGI CCWGG 1 cut(s) 44
PspPI GGNCC 2 cut(s) 48, 57
PsuI RGATCY 2 cut(s) 144, 455
RsaI GTAC 2 cut(s) 10, 196
RsaNI GTAC 2 cut(s) 9, 195
SacI GAGCTC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 100
SatI GCNGC 3 cut(s) 110, 113, 425
Sau3AI GATC 2 cut(s) 144, 455
Sau96I GGNCC 2 cut(s) 48, 57
ScrFI CCNGG 2 cut(s) 46, 60
SduI GDGCHC 1 cut(s) 173
SetI ASST 7 cut(s) 95, 173, 200, 349, 382, 469, 482
SfaNI GCATC 2 cut(s) 38, 297
SfcI CTRYAG 1 cut(s) 24
SfiI GGCCNNNNNGGCC 1 cut(s) 55
Sse9I AATT 3 cut(s) 243, 398, 494
SsiI CCGC 4 cut(s) 107, 110, 113, 166
SspI AATATT 1 cut(s) 394
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 2 cut(s) 44, 58
TaaI ACNGT 3 cut(s) 232, 287, 325
TaiI ACGT 1 cut(s) 200
TasI AATT 3 cut(s) 243, 398, 494
TatI WGTACW 1 cut(s) 8
TauI GCSGC 2 cut(s) 112, 115
TfiI GAWTC 2 cut(s) 186, 295
TscAI CASTG 2 cut(s) 372, 455
TseFI GTSAC 2 cut(s) 128, 363
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 2 cut(s) 128, 363
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 2 cut(s) 372, 455
XapI RAATTY 1 cut(s) 494
XmnI GAANNNNTTC 2 cut(s) 94, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.