Rh3AG277800

Polyketide cyclase / dehydrase and lipid transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0004371
Physical Location & Seq
Reverse (-)
32173924 .. 32180453
6530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG277800.1

Sequence Viewer

Length: 471 bp
ATGTCTGCTGCTTCTGCAACCGCTGTAGCAGCCTACATTTCTAGACCAAACTGTACCAGTCTCAGCCACGTACCCGTTTGGAGAAGCTCTTCTTCTCTAAAACCACAGCCGTCTCTTACTCGCATCTTCTTCAAAATCCCATGTGGGTTTTCCAAGCGAAGCTCAGTCTTCACTGGATTCAGACCTCTTTCCCCTGTTATGGAATGGCAGGACTGCACGGTAAAGACGAAAATTGATGTGCCTATTTCAGTCGCATATGATTGTTACTCTGATCGTGAGGCCATTCCCCAGTGGATGCCCTTTATTTCTACTGTAAAGGTATTGGAAGACAAGCCTGACCTATCACGATGGTCACTGAAGTATAGAGCTTTTGGGCGTGATATTGAATTCTCATGGCTTGCTCGAAATATGCAGCCCACCCGAAATCAGAAAATCCACTGGAGATCTCTGGAAGGTCTTCCTAACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc

Protein Analysis

156

Amino Acids

17.89

Weight (kDa)

9.85

Isoelectric Point (pI)

59.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 78 - 153 1.2e-10 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 21
AcsI RAATTY 1 cut(s) 386
AcuI CTGAAG 1 cut(s) 377
AfaI GTAC 2 cut(s) 55, 72
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 2 cut(s) 133, 386
AluBI AGCT 3 cut(s) 87, 162, 368
AluI AGCT 3 cut(s) 87, 162, 368
Alw26I GTCTC 2 cut(s) 65, 117
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 3 cut(s) 8, 29, 412
ApoI RAATTY 1 cut(s) 386
Asp700I GAANNNNTTC 2 cut(s) 88, 456
BbsI GAAGAC 3 cut(s) 160, 333, 449
BbvI GCAGC 2 cut(s) 41, 424
BccI CCATC 1 cut(s) 342
BceAI ACGGC 1 cut(s) 94
BcoDI GTCTC 2 cut(s) 65, 117
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 24
BglII AGATCT 1 cut(s) 443
BisI GCNGC 3 cut(s) 9, 30, 413
BlsI GCNGC 3 cut(s) 10, 31, 414
BmrI ACTGGG 1 cut(s) 283
BmsI GCATC 2 cut(s) 132, 285
BmuI ACTGGG 1 cut(s) 283
BpiI GAAGAC 3 cut(s) 160, 333, 449
BpmI CTGGAG 1 cut(s) 460
BsaAI YACGTR 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 4 cut(s) 57, 178, 289, 443
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 76, 177
BseNI ACTGG 4 cut(s) 57, 178, 289, 443
BseXI GCAGC 2 cut(s) 41, 424
BsgI GTGCAG 1 cut(s) 199
BshFI GGCC 1 cut(s) 281
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 65, 117
BsmBI CGTCTC 1 cut(s) 117
BsnI GGCC 1 cut(s) 281
Bsp143I GATC 2 cut(s) 271, 443
BspACI CCGC 1 cut(s) 21
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 2 cut(s) 75, 176
BspQI GCTCTTC 1 cut(s) 94
BsrI ACTGG 4 cut(s) 57, 178, 289, 443
BssMI GATC 2 cut(s) 271, 443
Bst4CI ACNGT 3 cut(s) 53, 220, 313
Bst6I CTCTTC 1 cut(s) 94
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 62, 163
BstF5I GGATG 1 cut(s) 300
BstKTI GATC 2 cut(s) 274, 446
BstMAI GTCTC 2 cut(s) 65, 117
BstMBI GATC 2 cut(s) 271, 443
BstMWI GCNNNNNNNGC 2 cut(s) 14, 29
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 41, 424
BstV2I GAAGAC 3 cut(s) 160, 333, 449
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BsuRI GGCC 1 cut(s) 281
BtsCI GGATG 1 cut(s) 300
BtsIMutI CAGTG 4 cut(s) 171, 296, 353, 436
Cac8I GCNNGC 1 cut(s) 399
Csp6I GTAC 2 cut(s) 54, 71
CviAII CATG 2 cut(s) 141, 393
CviQI GTAC 2 cut(s) 54, 71
DdeI CTNAG 2 cut(s) 62, 163
DpnI GATC 2 cut(s) 273, 445
DpnII GATC 2 cut(s) 271, 443
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 386
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 2 cut(s) 144, 396
FaiI YATR 7 cut(s) 142, 200, 256, 258, 363, 394, 410
FalI AAGNNNNNCTT 2 cut(s) 76, 108
FatI CATG 2 cut(s) 140, 392
FauNDI CATATG 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 9, 30, 413
FokI GGATG 1 cut(s) 307
Fsp4HI GCNGC 3 cut(s) 9, 30, 413
FspBI CTAG 1 cut(s) 42
GluI GCNGC 3 cut(s) 9, 30, 413
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 1 cut(s) 281
Hin1II CATG 2 cut(s) 144, 396
HinfI GANTC 1 cut(s) 177
Hpy188I TCNGA 3 cut(s) 182, 271, 429
Hpy188III TCNNGA 4 cut(s) 42, 275, 345, 449
HpyAV CCTTC 1 cut(s) 446
HpyCH4III ACNGT 3 cut(s) 53, 220, 313
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 3 cut(s) 17, 216, 412
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 29
HpyF3I CTNAG 2 cut(s) 62, 163
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 2 cut(s) 144, 396
Kzo9I GATC 2 cut(s) 271, 443
LguI GCTCTTC 1 cut(s) 94
LpnPI CCDG 9 cut(s) 70, 159, 194, 207, 302, 348, 424, 434, 451
Lsp1109I GCAGC 2 cut(s) 41, 424
LweI GCATC 2 cut(s) 132, 285
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 263, 351
MalI GATC 2 cut(s) 273, 445
MboI GATC 2 cut(s) 271, 443
MboII GAAGA 7 cut(s) 81, 84, 118, 121, 160, 338, 449
MflI RGATCY 1 cut(s) 443
MluCI AATT 2 cut(s) 231, 386
MnlI CCTC 2 cut(s) 195, 271
MroXI GAANNNNTTC 2 cut(s) 88, 456
MspA1I CMGCKG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 14, 29
NdeI CATATG 1 cut(s) 256
NdeII GATC 2 cut(s) 271, 443
NlaIII CATG 2 cut(s) 144, 396
NmuCI GTSAC 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PdmI GAANNNNTTC 2 cut(s) 88, 456
PfeI GAWTC 1 cut(s) 177
PkrI GCNGC 3 cut(s) 10, 31, 414
Ppu21I YACGTR 1 cut(s) 70
PsuI RGATCY 1 cut(s) 443
RsaI GTAC 2 cut(s) 55, 72
RsaNI GTAC 2 cut(s) 54, 71
SapI GCTCTTC 1 cut(s) 94
SatI GCNGC 3 cut(s) 9, 30, 413
Sau3AI GATC 2 cut(s) 271, 443
SetI ASST 9 cut(s) 72, 89, 164, 187, 321, 342, 370, 457, 470
SfaNI GCATC 2 cut(s) 132, 285
SfcI CTRYAG 1 cut(s) 24
Sse9I AATT 2 cut(s) 231, 386
SsiI CCGC 1 cut(s) 21
SspMI CTAG 1 cut(s) 42
TaaI ACNGT 3 cut(s) 53, 220, 313
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 403
TasI AATT 2 cut(s) 231, 386
TfiI GAWTC 1 cut(s) 177
TscAI CASTG 4 cut(s) 178, 296, 360, 443
TseFI GTSAC 1 cut(s) 351
TseI GCWGC 3 cut(s) 8, 29, 412
Tsp45I GTSAC 1 cut(s) 351
TspRI CASTG 4 cut(s) 178, 296, 360, 443
XapI RAATTY 1 cut(s) 386
XbaI TCTAGA 1 cut(s) 41
XmnI GAANNNNTTC 2 cut(s) 88, 456
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.