Prupe.2G148100_v2.0.a1

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0012482
Physical Location & Seq
Forward (+)
20403542 .. 20406569
3028 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G148100.1

Sequence Viewer

Length: 1335 bp
ATGACAATGGAGGAGGAGGGTGGTGGTAAGGTGAAGCTTGGTCTTGGGATTGGGGTTGATTGTTATGGTGATGATCATAATGGGACTGAAGAAGTTGTTGTTGGGAAGAAGAAGAAGAAGTATGGTGGTGGTGGGTTGACAGCTTCTCAGTTGCATGAGCTGGAGCAACAAGCGCTTATCTACAAGCACTTCGCTGCTAACCTTCCTGTTCCTTTTCACCTTGTGCTTCCCATTTGGAAGAGTGTGGCTGCCTCTTTTGGCTCCTCAAATGCTGCTGCTATCAACAGACAATACCCCAGTTGGTTCAGTGCAACGGGCTTTGACTATAGGGATCATGAACCAGGGAGGTGCAGAAGAACTGATGGAAAGAAATGGAGGTGCAATAAGAATGTAGTTCCTGATCAGAAATACTGTCAGCAACACATGCACAGAGGCCGCCAGCGTTCAAGAAAGCCTGTGGAAAATTCTGAAGTTGCTTCTCCTTCCAGCACTAAAACACCCAAGAATTCAGAAATTGAGTTAGGGAACTCAAAGACAAACCTTCAGATTTCAACCCCGAAAGGCCTTCAACTTATGGCACAATCCTACAATTATGTTAGTGTTAGCCAAGGTACAACCATGGCCTCAAGTGGCTATCATGAATGTAAGAAAAACATAAGACCTGTTACAACTACATCCATTGCCAATATACCTGCCAACAGATCTGCTACTAGTACCAACCCTGCTGCTGTAGCCACTACCACCATTGCTGGACCAACCACCACCACCAACAGCAGTACCAATGAAAATAGCCTCAATATTGGTGTAAAATACAATAGTAGCAACCATGCTGGTGGCAATTGCATGATCAGAGGAAGCAGCATGAATGTTGTTAATATATCACCAGGATTGGGCTTCTCCCCGAAGAGCGTTCTCCAAGCTCTTCTAGGTTGCAATGGCTTATACTTCGACAACGGAAGTGGAGTAGAACTTGAACCAGGGAGGTGTAGGAGAACGGATGGAAAGAAGTGGCGGTGCAGAAGGGATGTTCTTCCTGATCAGAAGTATTGTGGCCAGCATATCCACAGAGGGGCAAAGAGGCAAATGAAAGATGTCCAACCTGTTGCTGTTCCTTCTTCTTCAGCTGCTGCCATGAATACCACTCGGCTCTCTCGGACAACAGCCATATGCAGAAAAACTAATTGCGCAATCCCTAATACAAATCTCAGCATCTCCATCCCAGCAAATCCCCCACCGGGACGAAATGATGAAAGAATCAATTCTAGCAGCGATAGTGACACGACTCTTACAGATACCAGCCTTACTGCTTGTGACAGTAGTCATGTTTCTTCTTAG

Protein Analysis

445

Amino Acids

47.93

Weight (kDa)

9.47

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1186
Acc36I ACCTGC 1 cut(s) 700
AciI CCGC 2 cut(s) 436, 1012
AclWI GGATC 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 1051
AcsI RAATTY 2 cut(s) 463, 505
AcuI CTGAAG 4 cut(s) 108, 489, 527, 1104
AdeI CACNNNGTG 1 cut(s) 223
AfaI GTAC 3 cut(s) 613, 715, 778
AfeI AGCGCT 1 cut(s) 174
AfiI CCNNNNNNNGG 3 cut(s) 890, 1069, 1235
AgsI TTSAA 4 cut(s) 447, 552, 569, 974
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 3 cut(s) 340, 883, 976
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AluBI AGCT 5 cut(s) 37, 143, 160, 920, 1124
AluI AGCT 5 cut(s) 37, 143, 160, 920, 1124
AlwI GGATC 1 cut(s) 339
AlwNI CAGNNNCTG 1 cut(s) 1127
Aor51HI AGCGCT 1 cut(s) 174
AoxI GGCC 4 cut(s) 433, 562, 621, 1051
ApeKI GCWGC 9 cut(s) 194, 248, 272, 275, 725, 858, 1124, 1127, 1266
ApoI RAATTY 2 cut(s) 463, 505
Asp700I GAANNNNTTC 1 cut(s) 1258
AspLEI GCGC 2 cut(s) 175, 1187
AspS9I GGNCC 1 cut(s) 752
AsuC2I CCSGG 1 cut(s) 1236
AsuHPI GGTGA 4 cut(s) 43, 80, 209, 873
AvaII GGWCC 1 cut(s) 752
BaeI ACNNNNGTAYC 2 cut(s) 760, 793
BalI TGGCCA 1 cut(s) 1053
BbvI GCAGC 9 cut(s) 181, 235, 259, 262, 712, 870, 1111, 1114, 1278
BccI CCATC 3 cut(s) 356, 992, 1223
BciT130I CCWGG 3 cut(s) 342, 885, 978
BclI TGATCA 4 cut(s) 73, 400, 846, 1036
BcnI CCSGG 1 cut(s) 1236
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 3 cut(s) 711, 926, 1263
BfmI CTRYAG 2 cut(s) 325, 729
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 700
BglII AGATCT 1 cut(s) 701
Bme1390I CCNGG 4 cut(s) 342, 885, 978, 1236
Bme18I GGWCC 1 cut(s) 752
BmgT120I GGNCC 1 cut(s) 752
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 4 cut(s) 342, 885, 978, 1236
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 1218
BmuI ACTGGG 1 cut(s) 291
BoxI GACNNNNGTC 1 cut(s) 1317
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 610
BpuMI CCSGG 1 cut(s) 1236
BsaJI CCNNGG 4 cut(s) 341, 607, 618, 977
Bsc4I CCNNNNNNNGG 3 cut(s) 890, 1069, 1235
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 3 cut(s) 678, 744, 940
BseBI CCWGG 3 cut(s) 342, 885, 978
BseDI CCNNGG 4 cut(s) 341, 607, 618, 977
BseGI GGATG 4 cut(s) 674, 1003, 1030, 1215
BseLI CCNNNNNNNGG 3 cut(s) 890, 1069, 1235
BseMI GCAATG 3 cut(s) 678, 744, 940
BseMII CTCAG 2 cut(s) 161, 1219
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 3 cut(s) 26, 29, 253
BseXI GCAGC 9 cut(s) 181, 235, 259, 262, 712, 870, 1111, 1114, 1278
BseYI CCCAGC 1 cut(s) 1219
BsgI GTGCAG 2 cut(s) 370, 1036
BshFI GGCC 4 cut(s) 435, 564, 623, 1053
BsiSI CCGG 1 cut(s) 1235
BslFI GGGAC 2 cut(s) 97, 1251
BslI CCNNNNNNNGG 3 cut(s) 890, 1069, 1235
BsmFI GGGAC 2 cut(s) 97, 1251
BsnI GGCC 4 cut(s) 435, 564, 623, 1053
Bsp143I GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 2 cut(s) 436, 1012
BspANI GGCC 4 cut(s) 435, 564, 623, 1053
BspCNI CTCAG 2 cut(s) 160, 1218
BspHI TCATGA 2 cut(s) 334, 637
BspLI GGNNCC 1 cut(s) 262
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 1 cut(s) 339
BspQI GCTCTTC 2 cut(s) 899, 927
BsrDI GCAATG 3 cut(s) 678, 744, 940
BsrI ACTGG 1 cut(s) 297
BssECI CCNNGG 4 cut(s) 341, 607, 618, 977
BssMI GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
BssT1I CCWWGG 2 cut(s) 607, 618
Bst2UI CCWGG 3 cut(s) 342, 885, 978
Bst4CI ACNGT 2 cut(s) 413, 1316
Bst6I CTCTTC 3 cut(s) 233, 899, 927
BstAPI GCANNNNNTGC 1 cut(s) 424
BstC8I GCNNGC 2 cut(s) 440, 1055
BstDEI CTNAG 3 cut(s) 147, 1205, 1332
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 4 cut(s) 674, 1003, 1030, 1215
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 1187
BstKTI GATC 6 cut(s) 76, 334, 403, 704, 849, 1039
BstMBI GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 172, 424, 731
BstNI CCWGG 3 cut(s) 342, 885, 978
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 1317
BstSCI CCNGG 4 cut(s) 340, 883, 976, 1234
BstSFI CTRYAG 2 cut(s) 325, 729
BstV1I GCAGC 9 cut(s) 181, 235, 259, 262, 712, 870, 1111, 1114, 1278
BstX2I RGATCY 1 cut(s) 701
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 1 cut(s) 701
BsuRI GGCC 4 cut(s) 435, 564, 623, 1053
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 4 cut(s) 674, 1003, 1030, 1215
BtsIMutI CAGTG 1 cut(s) 313
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 2 cut(s) 440, 1055
CaiI CAGNNNCTG 1 cut(s) 1127
CciI TCATGA 2 cut(s) 334, 637
CfoI GCGC 2 cut(s) 175, 1187
Cfr13I GGNCC 1 cut(s) 752
Csp6I GTAC 3 cut(s) 612, 714, 777
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
CviQI GTAC 3 cut(s) 612, 714, 777
DdeI CTNAG 3 cut(s) 147, 1205, 1332
DpnI GATC 6 cut(s) 75, 333, 402, 703, 848, 1038
DpnII GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
DraIII CACNNNGTG 1 cut(s) 223
EaeI YGGCCR 1 cut(s) 1051
Eam1104I CTCTTC 3 cut(s) 233, 899, 927
EarI CTCTTC 3 cut(s) 233, 899, 927
Eco130I CCWWGG 2 cut(s) 607, 618
Eco147I AGGCCT 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 752
Eco47III AGCGCT 1 cut(s) 174
Eco57I CTGAAG 4 cut(s) 108, 489, 527, 1104
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 3 cut(s) 340, 883, 976
EcoT14I CCWWGG 2 cut(s) 607, 618
ErhI CCWWGG 2 cut(s) 607, 618
FaqI GGGAC 2 cut(s) 97, 1251
FauNDI CATATG 1 cut(s) 1166
FbaI TGATCA 4 cut(s) 73, 400, 846, 1036
FokI GGATG 4 cut(s) 661, 1010, 1037, 1202
FspBI CTAG 3 cut(s) 711, 926, 1263
FspI TGCGCA 1 cut(s) 1186
GlaI GCGC 2 cut(s) 174, 1186
GsaI CCCAGC 1 cut(s) 1223
GsuI CTGGAG 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 4 cut(s) 435, 564, 623, 1053
HapII CCGG 1 cut(s) 1235
HhaI GCGC 2 cut(s) 175, 1187
Hin6I GCGC 2 cut(s) 173, 1185
HinP1I GCGC 2 cut(s) 173, 1185
HincII GTYRAC 1 cut(s) 138
HindII GTYRAC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 2 cut(s) 1254, 1282
HpaII CCGG 1 cut(s) 1235
HphI GGTGA 4 cut(s) 43, 80, 209, 873
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 7 cut(s) 405, 469, 511, 546, 851, 1041, 1155
Hpy188III TCNNGA 5 cut(s) 335, 398, 447, 638, 1034
Hpy8I GTNNAC 1 cut(s) 138
HpyAV CCTTC 6 cut(s) 212, 492, 551, 575, 1014, 1122
HpyCH4III ACNGT 2 cut(s) 413, 1316
HpyCH4V TGCA 9 cut(s) 154, 311, 351, 381, 427, 843, 933, 1017, 1170
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 424, 731
HpyF3I CTNAG 3 cut(s) 147, 1205, 1332
HspAI GCGC 2 cut(s) 173, 1185
Ksp22I TGATCA 4 cut(s) 73, 400, 846, 1036
Kzo9I GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
LguI GCTCTTC 2 cut(s) 899, 927
LmnI GCTCC 2 cut(s) 163, 266
Lsp1109I GCAGC 9 cut(s) 181, 235, 259, 262, 712, 870, 1111, 1114, 1278
LweI GCATC 1 cut(s) 1218
MaeI CTAG 3 cut(s) 711, 926, 1263
MaeIII GTNAC 3 cut(s) 664, 1274, 1310
MalI GATC 6 cut(s) 75, 333, 402, 703, 848, 1038
MboI GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 1 cut(s) 701
MlsI TGGCCA 1 cut(s) 1053
MluCI AATT 7 cut(s) 463, 505, 513, 589, 838, 1180, 1258
MluNI TGGCCA 1 cut(s) 1053
MlyI GAGTC 1 cut(s) 1276
MmeI TCCRAC 1 cut(s) 1120
Mox20I TGGCCA 1 cut(s) 1053
MroXI GAANNNNTTC 1 cut(s) 1258
MscI TGGCCA 1 cut(s) 1053
MseI TTAA 1 cut(s) 873
MslI CAYNNNNRTG 1 cut(s) 831
Msp20I TGGCCA 1 cut(s) 1053
MspA1I CMGCKG 1 cut(s) 1124
MspI CCGG 1 cut(s) 1235
MspR9I CCNGG 4 cut(s) 342, 885, 978, 1236
MunI CAATTG 1 cut(s) 838
MvaI CCWGG 3 cut(s) 342, 885, 978
MwoI GCNNNNNNNGC 3 cut(s) 172, 424, 731
NciI CCSGG 1 cut(s) 1236
NcoI CCATGG 1 cut(s) 618
NdeI CATATG 1 cut(s) 1166
NdeII GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
NlaIV GGNNCC 1 cut(s) 262
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 2 cut(s) 1274, 1310
NsbI TGCGCA 1 cut(s) 1186
NspI RCATGY 1 cut(s) 427
PagI TCATGA 2 cut(s) 334, 637
PceI AGGCCT 1 cut(s) 564
PciSI GCTCTTC 2 cut(s) 899, 927
PdmI GAANNNNTTC 1 cut(s) 1258
PfeI GAWTC 1 cut(s) 1254
PleI GAGTC 1 cut(s) 1276
PpsI GAGTC 1 cut(s) 1276
PshAI GACNNNNGTC 1 cut(s) 1317
Psp6I CCWGG 3 cut(s) 340, 883, 976
PspFI CCCAGC 1 cut(s) 1219
PspGI CCWGG 3 cut(s) 340, 883, 976
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 1127
PsuI RGATCY 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 3 cut(s) 613, 715, 778
RsaNI GTAC 3 cut(s) 612, 714, 777
RseI CAYNNNNRTG 1 cut(s) 831
SapI GCTCTTC 2 cut(s) 899, 927
SaqAI TTAA 1 cut(s) 873
Sau3AI GATC 6 cut(s) 73, 331, 400, 701, 846, 1036
Sau96I GGNCC 1 cut(s) 752
SchI GAGTC 1 cut(s) 1276
ScrFI CCNGG 4 cut(s) 342, 885, 978, 1236
SfaNI GCATC 1 cut(s) 1218
SfcI CTRYAG 2 cut(s) 325, 729
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 831
SmlI CTYRAG 1 cut(s) 625
SmoI CTYRAG 1 cut(s) 625
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 7 cut(s) 463, 505, 513, 589, 838, 1180, 1258
SseBI AGGCCT 1 cut(s) 564
SsiI CCGC 2 cut(s) 436, 1012
SspI AATATT 1 cut(s) 799
SspMI CTAG 3 cut(s) 711, 926, 1263
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 4 cut(s) 340, 883, 976, 1234
StyI CCWWGG 2 cut(s) 607, 618
TaaI ACNGT 2 cut(s) 413, 1316
TaqI TCGA 1 cut(s) 948
TasI AATT 7 cut(s) 463, 505, 513, 589, 838, 1180, 1258
TauI GCSGC 1 cut(s) 438
TfiI GAWTC 1 cut(s) 1254
Tru1I TTAA 1 cut(s) 873
Tru9I TTAA 1 cut(s) 873
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 2 cut(s) 1274, 1310
TseI GCWGC 9 cut(s) 194, 248, 272, 275, 725, 858, 1124, 1127, 1266
Tsp45I GTSAC 2 cut(s) 1274, 1310
TspDTI ATGAA 7 cut(s) 351, 654, 798, 878, 1100, 1148, 1263
TspGWI ACGGA 2 cut(s) 969, 1010
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 752
XapI RAATTY 2 cut(s) 463, 505
XceI RCATGY 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 1258
XspI CTAG 3 cut(s) 711, 926, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.