pycom02g16430

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012482
Physical Location & Seq
Reverse (-)
13577598 .. 13580385
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g16430.2

Sequence Viewer

Length: 1395 bp
ATGGAGGATGGAATTGTGAGGAGAGCGAAGGAGGAGAGAGAAGAGGTGAAACTTGGACTTGGTATTGGGGTTGGTTGTAACTATGGTGGTCAGAACGGGAAGAAGAAGAAGATGAAGTGTGGTGGCGGTGGTGGCGGCGGCTGTGGTGGTGGGTTTACGGCTGCGCAGCTGCATGAGCTGGAGGAACAAGCGCTTATCTATAAGCACTTAGCTGCCGGGGTACCAGTGCCGGTTCAGCTTTTGGTTCCAATATGGAACAGTGTGGCTGCCTCTTTTGGCTCCTCAAATGCTGCTGCTATCTATCAGCACTTCCCCACCTTTATAGGATTCAATCCTGTGGGGGGCTTTGACTATCGAAGTAAAATGGATCCTGAGCTGGGAAGGTGCAGAAGAACAGATGGAAAGAAATGGAGGTGTAGTAAGAGTGTAGTTCCTGGTCAGAAATACTGTCAGCAACACATGCACAGAGGCTGCCAGCGTTCAAGAAAGCATGTGGAAACTTCTGAAACCTCTTCTCCTTCCAGCACAAAACAACCCAAGAATTCAGATGCTGAGTTGTGCAGTTCAAAGACAAACGTTCAGATTTCCACTCCGAATGGCCTTCATCTTAAGGCCCAATCCTCGGATAATATTGGTGTCAGCCAAGGTACAACCACTGCCTCCAATGGCTATCAAGAATGTAAGACGAACATCCGATCTGTTAGAACTACATCCGTTGCCATGATACCTGCCAATAGAACTGCTACATCCCCTGCTTCTGCAGTCACTATTGCGCATGCTGGACCAACTGCTGCCACCAACGGCAGTAGCAACAAAAATAGCATCAGCATTGGTGTAAAAGACTATAGTAGCAGCCATGCTGGTGACAATTGCATGATCAGAGGAAGCAGCCTCAACAGAAATAGCACCATTGTTGGTAGTACTATATCACCAGGATTGGGCTTCTCCCCAAAGAGCGTTCTTCAGGGTAGACAAATGACTTTTCTAATTTCCAATGGCGTATGCTTCAACAATAGTAGCGGGACAGAGCTTGAACCAAGGAGGTGTAGGAGAACGGATGGAAAGAAGTGGCGCTGCAGAAGGGATGTTCTTCCTGACCAGAAGTATTGTGGTCTGCATGTCCACAGAGGAGCAAAGAGGCAAACGAAAGACTCCCAGCCAATAGGTGTTCCTTCTAGTTCAGCTGCTTCCATGAACACCGCTCAGATCTCTCAGACGATATCCACTACCAAAAAAACTGATCGTGCAATCCCCAATACAAATCTCAGCATGTCCATCCCAGCAAGGCCCCCACCGATGCAAAATGATGAAAAAAGCAATTCCAGCGGCGATAGTGACACAACCATTACAGACACCAGCCTTGCTGCTTACGAGGGTAGTTATGTTTCTTCATAA

Protein Analysis

465

Amino Acids

49.43

Weight (kDa)

9.61

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 165, 774
Acc36I ACCTGC 1 cut(s) 736
Acc65I GGTACC 1 cut(s) 220
AccB1I GGYRCC 1 cut(s) 220
AccBSI CCGCTC 1 cut(s) 1202
AccI GTMKAC 1 cut(s) 970
AciI CCGC 6 cut(s) 126, 135, 138, 1020, 1200, 1326
AclI AACGTT 1 cut(s) 576
AclWI GGATC 2 cut(s) 362, 375
AcsI RAATTY 1 cut(s) 541
AcuI CTGAAG 1 cut(s) 947
AfaI GTAC 3 cut(s) 222, 649, 922
AfeI AGCGCT 1 cut(s) 192
AfiI CCNNNNNNNGG 4 cut(s) 341, 377, 622, 938
AflII CTTAAG 1 cut(s) 608
AgsI TTSAA 5 cut(s) 331, 483, 567, 1009, 1034
AjnI CCWGG 2 cut(s) 433, 931
AluBI AGCT 7 cut(s) 169, 178, 212, 238, 376, 1030, 1184
AluI AGCT 7 cut(s) 169, 178, 212, 238, 376, 1030, 1184
AlwI GGATC 2 cut(s) 362, 375
AlwNI CAGNNNCTG 2 cut(s) 471, 551
Aor51HI AGCGCT 1 cut(s) 192
AoxI GGCC 3 cut(s) 598, 612, 1286
ApoI RAATTY 1 cut(s) 541
Asp718I GGTACC 1 cut(s) 220
AspLEI GCGC 4 cut(s) 166, 193, 775, 1074
AspS9I GGNCC 3 cut(s) 613, 782, 1287
AsuC2I CCSGG 1 cut(s) 217
AsuHPI GGTGA 3 cut(s) 58, 875, 921
AvaII GGWCC 1 cut(s) 782
BamHI GGATCC 1 cut(s) 367
BanI GGYRCC 1 cut(s) 220
BccI CCATC 4 cut(s) 2, 392, 1052, 1283
BceAI ACGGC 2 cut(s) 174, 817
BciT130I CCWGG 2 cut(s) 435, 933
BclI TGATCA 1 cut(s) 876
BcnI CCSGG 1 cut(s) 217
BfaI CTAG 1 cut(s) 1176
BfmI CTRYAG 3 cut(s) 759, 844, 1075
BfoI RGCGCY 2 cut(s) 194, 1075
BfrI CTTAAG 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 736
BglII AGATCT 1 cut(s) 1206
BmcAI AGTACT 1 cut(s) 922
Bme1390I CCNGG 3 cut(s) 217, 435, 933
Bme18I GGWCC 1 cut(s) 782
BmgT120I GGNCC 3 cut(s) 613, 782, 1287
BmiI GGNNCC 5 cut(s) 222, 246, 280, 369, 1289
BmrFI CCNGG 3 cut(s) 217, 435, 933
BmsI GCATC 3 cut(s) 538, 831, 1287
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 372
BpuMI CCSGG 1 cut(s) 217
BsaJI CCNNGG 4 cut(s) 216, 621, 643, 1037
BsaXI ACNNNNNCTCC 4 cut(s) 403, 433, 499, 529
Bsc4I CCNNNNNNNGG 4 cut(s) 341, 377, 622, 938
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 224
BseBI CCWGG 2 cut(s) 435, 933
BseDI CCNNGG 4 cut(s) 216, 621, 643, 1037
BseGI GGATG 7 cut(s) 13, 690, 710, 746, 1063, 1090, 1275
BseLI CCNNNNNNNGG 4 cut(s) 341, 377, 622, 938
BseMII CTCAG 5 cut(s) 363, 543, 1217, 1226, 1279
BseNI ACTGG 1 cut(s) 224
BseRI GAGGAG 4 cut(s) 34, 47, 271, 1143
BseYI CCCAGC 3 cut(s) 376, 1155, 1279
BsgI GTGCAG 2 cut(s) 406, 580
BshFI GGCC 3 cut(s) 600, 614, 1288
BshNI GGYRCC 1 cut(s) 220
BsiSI CCGG 2 cut(s) 216, 230
BslFI GGGAC 1 cut(s) 1036
BslI CCNNNNNNNGG 4 cut(s) 341, 377, 622, 938
BsmFI GGGAC 1 cut(s) 1036
BsnI GGCC 3 cut(s) 600, 614, 1288
Bsp143I GATC 5 cut(s) 367, 695, 876, 1206, 1240
BspACI CCGC 6 cut(s) 126, 135, 138, 1020, 1200, 1326
BspANI GGCC 3 cut(s) 600, 614, 1288
BspCNI CTCAG 5 cut(s) 364, 544, 1216, 1225, 1278
BspLI GGNNCC 5 cut(s) 222, 246, 280, 369, 1289
BspMAI CTGCAG 2 cut(s) 763, 1079
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 2 cut(s) 362, 375
BspT107I GGYRCC 1 cut(s) 220
BspTI CTTAAG 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 1202
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 1 cut(s) 224
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 4 cut(s) 216, 621, 643, 1037
BssMI GATC 5 cut(s) 367, 695, 876, 1206, 1240
BssT1I CCWWGG 2 cut(s) 643, 1037
Bst2UI CCWGG 2 cut(s) 435, 933
Bst4CI ACNGT 2 cut(s) 260, 449
Bst6I CTCTTC 2 cut(s) 36, 517
BstAFI CTTAAG 1 cut(s) 608
BstAPI GCANNNNNTGC 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 476, 777
BstDEI CTNAG 6 cut(s) 208, 372, 552, 1203, 1212, 1265
BstF5I GGATG 7 cut(s) 13, 690, 710, 746, 1063, 1090, 1275
BstH2I RGCGCY 2 cut(s) 194, 1075
BstHHI GCGC 4 cut(s) 166, 193, 775, 1074
BstKTI GATC 5 cut(s) 370, 698, 879, 1209, 1243
BstMBI GATC 5 cut(s) 367, 695, 876, 1206, 1240
BstMWI GCNNNNNNNGC 5 cut(s) 132, 175, 235, 460, 1323
BstNI CCWGG 2 cut(s) 435, 933
BstNSI RCATGY 5 cut(s) 463, 494, 779, 1121, 1273
BstSCI CCNGG 3 cut(s) 215, 433, 931
BstSFI CTRYAG 3 cut(s) 759, 844, 1075
BstX2I RGATCY 2 cut(s) 367, 1206
BstYI RGATCY 2 cut(s) 367, 1206
BsuRI GGCC 3 cut(s) 600, 614, 1288
BtsCI GGATG 7 cut(s) 13, 690, 710, 746, 1063, 1090, 1275
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 3 cut(s) 231, 265, 654
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 2 cut(s) 476, 777
CaiI CAGNNNCTG 2 cut(s) 471, 551
CfoI GCGC 4 cut(s) 166, 193, 775, 1074
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 3 cut(s) 613, 782, 1287
Csp6I GTAC 3 cut(s) 221, 648, 921
CviQI GTAC 3 cut(s) 221, 648, 921
DdeI CTNAG 6 cut(s) 208, 372, 552, 1203, 1212, 1265
DpnI GATC 5 cut(s) 369, 697, 878, 1208, 1242
DpnII GATC 5 cut(s) 367, 695, 876, 1206, 1240
Eam1104I CTCTTC 2 cut(s) 36, 517
EarI CTCTTC 2 cut(s) 36, 517
Eco130I CCWWGG 2 cut(s) 643, 1037
Eco32I GATATC 1 cut(s) 1221
Eco47I GGWCC 1 cut(s) 782
Eco47III AGCGCT 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 947
EcoO109I RGGNCCY 1 cut(s) 1287
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 2 cut(s) 433, 931
EcoRV GATATC 1 cut(s) 1221
EcoT14I CCWWGG 2 cut(s) 643, 1037
ErhI CCWWGG 2 cut(s) 643, 1037
FaqI GGGAC 1 cut(s) 1036
FauI CCCGC 1 cut(s) 1013
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 1 cut(s) 970
FokI GGATG 7 cut(s) 20, 677, 697, 733, 1070, 1097, 1262
FspBI CTAG 1 cut(s) 1176
FspI TGCGCA 2 cut(s) 165, 774
GlaI GCGC 4 cut(s) 165, 192, 774, 1073
GsaI CCCAGC 3 cut(s) 380, 1159, 1283
GsuI CTGGAG 1 cut(s) 200
HaeII RGCGCY 2 cut(s) 194, 1075
HaeIII GGCC 3 cut(s) 600, 614, 1288
HapII CCGG 2 cut(s) 216, 230
HhaI GCGC 4 cut(s) 166, 193, 775, 1074
Hin6I GCGC 4 cut(s) 164, 191, 773, 1072
HinP1I GCGC 4 cut(s) 164, 191, 773, 1072
HinfI GANTC 2 cut(s) 327, 1151
HpaII CCGG 2 cut(s) 216, 230
HphI GGTGA 3 cut(s) 58, 875, 921
Hpy166II GTNNAC 3 cut(s) 156, 971, 1123
Hpy188III TCNNGA 4 cut(s) 371, 483, 674, 1094
Hpy8I GTNNAC 3 cut(s) 156, 971, 1123
HpyAV CCTTC 6 cut(s) 22, 375, 528, 611, 1074, 1182
HpyCH4III ACNGT 2 cut(s) 260, 449
HpyCH4IV ACGT 1 cut(s) 576
HpyF10VI GCNNNNNNNGC 5 cut(s) 132, 175, 235, 460, 1323
HpyF3I CTNAG 6 cut(s) 208, 372, 552, 1203, 1212, 1265
HpySE526I ACGT 1 cut(s) 576
HspAI GCGC 4 cut(s) 164, 191, 773, 1072
KpnI GGTACC 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 5 cut(s) 367, 695, 876, 1206, 1240
LmnI GCTCC 2 cut(s) 284, 1130
LweI GCATC 3 cut(s) 538, 831, 1287
MaeI CTAG 1 cut(s) 1176
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 4 cut(s) 77, 763, 863, 1334
MalI GATC 5 cut(s) 369, 697, 878, 1208, 1242
MbiI CCGCTC 1 cut(s) 1202
MboI GATC 5 cut(s) 367, 695, 876, 1206, 1240
MfeI CAATTG 1 cut(s) 868
MflI RGATCY 2 cut(s) 367, 1206
MluCI AATT 5 cut(s) 12, 541, 868, 987, 1318
MlyI GAGTC 1 cut(s) 1145
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 861
MspA1I CMGCKG 3 cut(s) 169, 1184, 1326
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 2 cut(s) 216, 230
MspR9I CCNGG 3 cut(s) 217, 435, 933
MunI CAATTG 1 cut(s) 868
MvaI CCWGG 2 cut(s) 435, 933
MwoI GCNNNNNNNGC 5 cut(s) 132, 175, 235, 460, 1323
NciI CCSGG 1 cut(s) 217
NdeII GATC 5 cut(s) 367, 695, 876, 1206, 1240
NlaIV GGNNCC 5 cut(s) 222, 246, 280, 369, 1289
NmuCI GTSAC 3 cut(s) 763, 863, 1334
NsbI TGCGCA 2 cut(s) 165, 774
NspI RCATGY 5 cut(s) 463, 494, 779, 1121, 1273
PaeI GCATGC 1 cut(s) 779
PfeI GAWTC 1 cut(s) 327
PleI GAGTC 1 cut(s) 1145
PpsI GAGTC 1 cut(s) 1145
Psp1406I AACGTT 1 cut(s) 576
Psp6I CCWGG 2 cut(s) 433, 931
PspFI CCCAGC 3 cut(s) 376, 1155, 1279
PspGI CCWGG 2 cut(s) 433, 931
PspN4I GGNNCC 5 cut(s) 222, 246, 280, 369, 1289
PspPI GGNCC 3 cut(s) 613, 782, 1287
PstI CTGCAG 2 cut(s) 763, 1079
PstNI CAGNNNCTG 2 cut(s) 471, 551
PsuI RGATCY 2 cut(s) 367, 1206
PvuII CAGCTG 2 cut(s) 169, 1184
RsaI GTAC 3 cut(s) 222, 649, 922
RsaNI GTAC 3 cut(s) 221, 648, 921
RseI CAYNNNNRTG 1 cut(s) 861
SaqAI TTAA 1 cut(s) 609
Sau3AI GATC 5 cut(s) 367, 695, 876, 1206, 1240
Sau96I GGNCC 3 cut(s) 613, 782, 1287
ScaI AGTACT 1 cut(s) 922
SchI GAGTC 1 cut(s) 1145
ScrFI CCNGG 3 cut(s) 217, 435, 933
SfaNI GCATC 3 cut(s) 538, 831, 1287
SfcI CTRYAG 3 cut(s) 759, 844, 1075
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
SphI GCATGC 1 cut(s) 779
Sse9I AATT 5 cut(s) 12, 541, 868, 987, 1318
SsiI CCGC 6 cut(s) 126, 135, 138, 1020, 1200, 1326
SspI AATATT 1 cut(s) 631
SspMI CTAG 1 cut(s) 1176
StyD4I CCNGG 3 cut(s) 215, 433, 931
StyI CCWWGG 2 cut(s) 643, 1037
TaaI ACNGT 2 cut(s) 260, 449
TaiI ACGT 1 cut(s) 579
TaqI TCGA 1 cut(s) 355
TasI AATT 5 cut(s) 12, 541, 868, 987, 1318
TatI WGTACW 1 cut(s) 920
TauI GCSGC 3 cut(s) 138, 141, 1329
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 3 cut(s) 231, 265, 661
TseFI GTSAC 3 cut(s) 763, 863, 1334
Tsp45I GTSAC 3 cut(s) 763, 863, 1334
TspDTI ATGAA 5 cut(s) 128, 593, 1208, 1323, 1380
TspGWI ACGGA 2 cut(s) 703, 1070
TspRI CASTG 3 cut(s) 231, 265, 661
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 1 cut(s) 541
XceI RCATGY 5 cut(s) 463, 494, 779, 1121, 1273
XcmI CCANNNNNNNNNTGG 1 cut(s) 1106
XmiI GTMKAC 1 cut(s) 970
XspI CTAG 1 cut(s) 1176
ZrmI AGTACT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.