RLG00000028446

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012482
Physical Location & Seq
Reverse (-)
22941654 .. 22943803
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028446

Sequence Viewer

Length: 1341 bp
ATGAACGACACCCAAGTCGTGAAGGTCAAGCATGAGGATGATGAAGGATCGCCGCCGCCGCCCTCGATCAAGCTCGGTCTGGCGATCGGCGGTGATGGTGGTGACTGTGAAGTAGAGGGGAAGAGGAGGAGGAGGAGTGAGTTTACGGTGGCTCAGTTGGATGAGTTGGAGCAGCAGAGGCTTATTTATAAGTACATAGCTGCTGGATTGCCTGCTCCTTTTCAGCTTGTGCTTCCCATTTGGAAGAGTGTGGCTGCCGGTTTTTCTAATGCTGCTTTCTACCAGCAATATCCCAGCTTTCTAGGATATGGGGGTTTCGACTACAGGAGTAGAATGGATACTGAGCCAGGAAGGTGTAGAAGAACTGATGGAAAGAAATGGAGGTGTAGTAGGGATGTAGTGCCTGATGAGAAGTACTGTGAGAAACACATGCACAGAGGGCGCCATCGTTCAAGAAAGCCTGTGGAAACTTCTCAAGTCACTTCGCCTCCCAGCACAAAGCTACCGAAGAATTCAGACGGCAAAATGTTGAATTCAAAGAAAAACCTTCAGATTCAAACCCCCAAAGGCCTTCAACTTATGGCGCAACCCTCCAATAATGGTAGTGTCAGCCACAGTCGAACCACCTTCTCTAGTGGCTATCATGAGTTACCTAAGAACATTAGTACAGTTGCACACACTACCTGCACACCTTCATACAGAACTGCTGTTACCCCTGCTGCTGTAGCCCCTTCCACCACTGCTGGACAAATGACCACAACCGCCAGCACTTGCAATGACAATAACATCTGGATTGGTGTGAAAGACTATGGTTGCAGCTACCATGCTGGTAATTATGGCATTATCAGAGGAAGCAGAAACAACATCAATGTTGGCAGTAATATATCGCCCGGATCGGGCTTCTCCCCCAAAAGTGTTCTTCAGGCAGGACATATGATTCTTGTAAATTGCAATGGCTTATACTTTGACAACAGACGTGGCGAAGAACTTGAACCAGGCAGGTGTAGGAGGACAGATGGAAAGAAGTGGCGCTGCAAAAGGGAAGTTCTTCCTGATCAGAAGTACTGTGACCGGCATATCCACAGAGGAGTAAAGCAAATGAGAACCTTTGGCCCTGTTACTGCTGCTTCTACCTCGCACACTGCAATGAATTCTACTCGGCCCTCTCAGACCACAGGCATAGCTGGAGAAACTGATTGTGCAATCCCTAATACAAATCTCACCATGTCCATCCCAGCAAGATCACCACTGATGCAAAATGATGAAAAAAGTGATTCCACCAGCGACAGTGACGCAACCATTACAGACACCAGCCTTGCTGCTTATGAACAGCTGATTTAG

Protein Analysis

447

Amino Acids

49.09

Weight (kDa)

9.25

Isoelectric Point (pI)

54.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 48 - 81 1.4e-10 QLQ
WRC PF08879 115 - 156 3e-21 WRC
WRC PF08879 331 - 366 2e-18 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 189
AarI CACCTGC 1 cut(s) 990
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 2 cut(s) 692, 990
AccB1I GGYRCC 1 cut(s) 441
AciI CCGC 5 cut(s) 53, 56, 59, 90, 762
AclWI GGATC 2 cut(s) 55, 901
AcsI RAATTY 3 cut(s) 511, 532, 1150
AcuI CTGAAG 2 cut(s) 533, 905
AcyI GRCGYC 1 cut(s) 442
AfaI GTAC 4 cut(s) 194, 416, 667, 1064
AfiI CCNNNNNNNGG 1 cut(s) 896
AgsI TTSAA 6 cut(s) 453, 532, 537, 557, 575, 992
AjiI CACGTC 1 cut(s) 977
AjnI CCWGG 2 cut(s) 346, 994
AluBI AGCT 8 cut(s) 73, 200, 226, 297, 502, 819, 1184, 1333
AluI AGCT 8 cut(s) 73, 200, 226, 297, 502, 819, 1184, 1333
AlwI GGATC 2 cut(s) 55, 901
AoxI GGCC 3 cut(s) 568, 1111, 1160
ApeKI GCWGC 9 cut(s) 172, 200, 254, 272, 719, 816, 1032, 1124, 1319
ApoI RAATTY 3 cut(s) 511, 532, 1150
Asp700I GAANNNNTTC 1 cut(s) 1047
AspLEI GCGC 3 cut(s) 444, 586, 1032
AspS9I GGNCC 2 cut(s) 1112, 1161
AsuC2I CCSGG 1 cut(s) 891
AsuHPI GGTGA 4 cut(s) 104, 113, 1213, 1236
BanI GGYRCC 1 cut(s) 441
BbvI GCAGC 9 cut(s) 184, 187, 241, 259, 706, 828, 1019, 1111, 1306
BccI CCATC 5 cut(s) 89, 362, 453, 1010, 1238
BceAI ACGGC 1 cut(s) 535
BciT130I CCWGG 2 cut(s) 348, 996
BciVI GTATCC 1 cut(s) 331
BclI TGATCA 1 cut(s) 1054
BcnI CCSGG 1 cut(s) 891
BfaI CTAG 2 cut(s) 302, 633
BfmI CTRYAG 2 cut(s) 322, 723
BfoI RGCGCY 2 cut(s) 445, 1033
BfuAI ACCTGC 2 cut(s) 692, 990
BfuI GTATCC 1 cut(s) 331
BmcAI AGTACT 2 cut(s) 416, 1064
Bme1390I CCNGG 3 cut(s) 348, 891, 996
BmgBI CACGTC 1 cut(s) 977
BmgT120I GGNCC 2 cut(s) 1112, 1161
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 3 cut(s) 348, 891, 996
BmsI GCATC 1 cut(s) 1242
BpmI CTGGAG 1 cut(s) 1206
BpuEI CTTGAG 1 cut(s) 459
BpuMI CCSGG 1 cut(s) 891
BsaHI GRCGYC 1 cut(s) 442
BsaXI ACNNNNNCTCC 6 cut(s) 121, 151, 373, 403, 472, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 896
Bse118I RCCGGY 2 cut(s) 257, 1071
Bse3DI GCAATG 3 cut(s) 781, 958, 1152
BseBI CCWGG 2 cut(s) 348, 996
BseGI GGATG 4 cut(s) 43, 166, 400, 1230
BseLI CCNNNNNNNGG 1 cut(s) 896
BseMI GCAATG 3 cut(s) 781, 958, 1152
BseMII CTCAG 3 cut(s) 167, 333, 1181
BseRI GAGGAG 5 cut(s) 139, 142, 145, 148, 1101
BseXI GCAGC 9 cut(s) 184, 187, 241, 259, 706, 828, 1019, 1111, 1306
BseYI CCCAGC 3 cut(s) 293, 491, 1234
BsgI GTGCAG 1 cut(s) 670
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 3 cut(s) 570, 1113, 1162
BshNI GGYRCC 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 87
BsiSI CCGG 3 cut(s) 258, 891, 1072
BslI CCNNNNNNNGG 1 cut(s) 896
BsnI GGCC 3 cut(s) 570, 1113, 1162
Bsp143I GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
BspACI CCGC 5 cut(s) 53, 56, 59, 90, 762
BspANI GGCC 3 cut(s) 570, 1113, 1162
BspCNI CTCAG 3 cut(s) 166, 334, 1180
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 443
BspMI ACCTGC 2 cut(s) 692, 990
BspPI GGATC 2 cut(s) 55, 901
BspT107I GGYRCC 1 cut(s) 441
BsrDI GCAATG 3 cut(s) 781, 958, 1152
BsrFI RCCGGY 2 cut(s) 257, 1071
BssAI RCCGGY 2 cut(s) 257, 1071
BssMI GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
BssNI GRCGYC 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 348, 996
Bst4CI ACNGT 7 cut(s) 107, 148, 419, 617, 670, 1067, 1289
Bst6I CTCTTC 2 cut(s) 116, 239
BstACI GRCGYC 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 213, 766
BstDEI CTNAG 4 cut(s) 153, 342, 654, 1167
BstF5I GGATG 4 cut(s) 43, 166, 400, 1230
BstH2I RGCGCY 2 cut(s) 445, 1033
BstHHI GCGC 3 cut(s) 444, 586, 1032
BstKTI GATC 6 cut(s) 50, 69, 87, 896, 1057, 1244
BstMBI GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 4 cut(s) 58, 178, 439, 725
BstNI CCWGG 2 cut(s) 348, 996
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 3 cut(s) 346, 889, 994
BstSFI CTRYAG 2 cut(s) 322, 723
BstV1I GCAGC 9 cut(s) 184, 187, 241, 259, 706, 828, 1019, 1111, 1306
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 3 cut(s) 570, 1113, 1162
BtrI CACGTC 1 cut(s) 977
BtsCI GGATG 4 cut(s) 43, 166, 400, 1230
BtsI GCAGTG 2 cut(s) 738, 1140
BtsIMutI CAGTG 4 cut(s) 738, 1140, 1247, 1294
BveI ACCTGC 2 cut(s) 692, 990
Cac8I GCNNGC 2 cut(s) 213, 766
CciI TCATGA 1 cut(s) 643
CfoI GCGC 3 cut(s) 444, 586, 1032
Cfr10I RCCGGY 2 cut(s) 257, 1071
Cfr13I GGNCC 2 cut(s) 1112, 1161
CseI GACGC 1 cut(s) 1301
Csp6I GTAC 4 cut(s) 193, 415, 666, 1063
CspCI CAANNNNNGTGG 2 cut(s) 958, 993
CviAII CATG 5 cut(s) 32, 430, 644, 824, 1225
CviQI GTAC 4 cut(s) 193, 415, 666, 1063
DdeI CTNAG 4 cut(s) 153, 342, 654, 1167
DinI GGCGCC 1 cut(s) 443
DpnI GATC 6 cut(s) 49, 68, 86, 895, 1056, 1243
DpnII GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
Eam1104I CTCTTC 2 cut(s) 116, 239
EarI CTCTTC 2 cut(s) 116, 239
Eco147I AGGCCT 1 cut(s) 570
Eco57I CTGAAG 2 cut(s) 533, 905
EcoRI GAATTC 3 cut(s) 511, 532, 1150
EcoRII CCWGG 2 cut(s) 346, 994
EgeI GGCGCC 1 cut(s) 443
EheI GGCGCC 1 cut(s) 443
FaeI CATG 5 cut(s) 35, 433, 647, 827, 1228
FatI CATG 5 cut(s) 31, 429, 643, 823, 1224
FauNDI CATATG 1 cut(s) 933
FbaI TGATCA 1 cut(s) 1054
FokI GGATG 4 cut(s) 50, 173, 407, 1217
FspBI CTAG 2 cut(s) 302, 633
GlaI GCGC 3 cut(s) 443, 585, 1031
GsaI CCCAGC 3 cut(s) 297, 495, 1238
GsuI CTGGAG 1 cut(s) 1206
HaeII RGCGCY 2 cut(s) 445, 1033
HaeIII GGCC 3 cut(s) 570, 1113, 1162
HapII CCGG 3 cut(s) 258, 891, 1072
HgaI GACGC 1 cut(s) 1301
HhaI GCGC 3 cut(s) 444, 586, 1032
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 5 cut(s) 35, 433, 647, 827, 1228
Hin6I GCGC 3 cut(s) 442, 584, 1030
HinP1I GCGC 3 cut(s) 442, 584, 1030
HinfI GANTC 3 cut(s) 553, 937, 1274
HpaII CCGG 3 cut(s) 258, 891, 1072
HphI GGTGA 4 cut(s) 104, 113, 1213, 1236
Hpy166II GTNNAC 1 cut(s) 144
Hpy188I TCNGA 5 cut(s) 517, 552, 848, 1059, 1170
Hpy188III TCNNGA 5 cut(s) 19, 453, 644, 790, 1052
Hpy8I GTNNAC 1 cut(s) 144
HpyAV CCTTC 8 cut(s) 16, 38, 345, 557, 581, 637, 702, 741
HpyCH4III ACNGT 7 cut(s) 107, 148, 419, 617, 670, 1067, 1289
HpyCH4IV ACGT 1 cut(s) 976
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 178, 439, 725
HpyF3I CTNAG 4 cut(s) 153, 342, 654, 1167
HpySE526I ACGT 1 cut(s) 976
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 5 cut(s) 35, 433, 647, 827, 1228
HspAI GCGC 3 cut(s) 442, 584, 1030
KasI GGCGCC 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
LmnI GCTCC 2 cut(s) 169, 220
Lsp1109I GCAGC 9 cut(s) 184, 187, 241, 259, 706, 828, 1019, 1111, 1306
LweI GCATC 1 cut(s) 1242
MaeI CTAG 2 cut(s) 302, 633
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 7 cut(s) 101, 478, 648, 709, 1067, 1117, 1289
MalI GATC 6 cut(s) 49, 68, 86, 895, 1056, 1243
MboI GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
MboII GAAGA 7 cut(s) 133, 256, 372, 520, 911, 995, 1040
MluCI AATT 5 cut(s) 511, 532, 832, 946, 1150
Mly113I GGCGCC 1 cut(s) 442
MmeI TCCRAC 2 cut(s) 138, 147
MroXI GAANNNNTTC 1 cut(s) 1047
MslI CAYNNNNRTG 2 cut(s) 36, 1145
MspA1I CMGCKG 1 cut(s) 1333
MspI CCGG 3 cut(s) 258, 891, 1072
MspR9I CCNGG 3 cut(s) 348, 891, 996
MvaI CCWGG 2 cut(s) 348, 996
MwoI GCNNNNNNNGC 4 cut(s) 58, 178, 439, 725
NarI GGCGCC 1 cut(s) 442
NciI CCSGG 1 cut(s) 891
NdeI CATATG 1 cut(s) 933
NdeII GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
NlaIII CATG 5 cut(s) 35, 433, 647, 827, 1228
NlaIV GGNNCC 1 cut(s) 443
NmeAIII GCCGAG 1 cut(s) 1138
NmuCI GTSAC 4 cut(s) 101, 478, 1067, 1289
NspI RCATGY 1 cut(s) 433
PagI TCATGA 1 cut(s) 643
PaqCI CACCTGC 1 cut(s) 990
PceI AGGCCT 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 1047
PfeI GAWTC 3 cut(s) 553, 937, 1274
Ple19I CGATCG 1 cut(s) 87
PluTI GGCGCC 1 cut(s) 445
PsiI TTATAA 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 346, 994
PspFI CCCAGC 3 cut(s) 293, 491, 1234
PspGI CCWGG 2 cut(s) 346, 994
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 2 cut(s) 1112, 1161
PsrI GAACNNNNNNTAC 2 cut(s) 694, 726
PvuI CGATCG 1 cut(s) 87
PvuII CAGCTG 1 cut(s) 1333
RsaI GTAC 4 cut(s) 194, 416, 667, 1064
RsaNI GTAC 4 cut(s) 193, 415, 666, 1063
RseI CAYNNNNRTG 2 cut(s) 36, 1145
Sau3AI GATC 6 cut(s) 47, 66, 84, 893, 1054, 1241
Sau96I GGNCC 2 cut(s) 1112, 1161
ScaI AGTACT 2 cut(s) 416, 1064
ScrFI CCNGG 3 cut(s) 348, 891, 996
SfaNI GCATC 1 cut(s) 1242
SfcI CTRYAG 2 cut(s) 322, 723
SfoI GGCGCC 1 cut(s) 443
SmiMI CAYNNNNRTG 2 cut(s) 36, 1145
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
Sse9I AATT 5 cut(s) 511, 532, 832, 946, 1150
SseBI AGGCCT 1 cut(s) 570
SsiI CCGC 5 cut(s) 53, 56, 59, 90, 762
SspDI GGCGCC 1 cut(s) 441
SspMI CTAG 2 cut(s) 302, 633
StuI AGGCCT 1 cut(s) 570
StyD4I CCNGG 3 cut(s) 346, 889, 994
TaaI ACNGT 7 cut(s) 107, 148, 419, 617, 670, 1067, 1289
TaiI ACGT 1 cut(s) 979
TaqI TCGA 3 cut(s) 65, 318, 619
TaqII GACCGA 1 cut(s) 65
TasI AATT 5 cut(s) 511, 532, 832, 946, 1150
TatI WGTACW 4 cut(s) 192, 414, 665, 1062
TauI GCSGC 3 cut(s) 55, 58, 61
TfiI GAWTC 3 cut(s) 553, 937, 1274
TscAI CASTG 4 cut(s) 745, 1147, 1254, 1294
TseFI GTSAC 4 cut(s) 101, 478, 1067, 1289
TseI GCWGC 9 cut(s) 172, 200, 254, 272, 719, 816, 1032, 1124, 1319
Tsp45I GTSAC 4 cut(s) 101, 478, 1067, 1289
TspDTI ATGAA 6 cut(s) 17, 57, 684, 1163, 1278, 1341
TspRI CASTG 4 cut(s) 745, 1147, 1254, 1294
XapI RAATTY 3 cut(s) 511, 532, 1150
XceI RCATGY 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 1047
XspI CTAG 2 cut(s) 302, 633
ZrmI AGTACT 2 cut(s) 416, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.