Rh1DG227200

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0012482
Physical Location & Seq
Forward (+)
43741443 .. 43744693
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG227200.1

Sequence Viewer

Length: 1017 bp
ATGAACGACACCCAAGTCGTGAAGGTCAAGCATGAGGATGATGAAGGATCGTCGCCGCCGTCGATCAAGCTCGGTCTTGCGATCGGCGGTGATGGTGGTGATTGTGAGGTAGTGGGGAAGAGGAGGAGGAGTGAGTTTACGGTGGCTCAGTTGGATGAGTTGGAGCAGCAGAGGCTTGTTTTTAAGTACATAGCTGCTGGGTTGCCTGCTCCTTTTCAGCTTGTGCTTCCCATTTGGAAGAGTGTGGCAGCCGGTTTTTCTAATGCTGCTTTCTACCAGCAATATCCCAGCTTTCTAGGATATGGGGGTTTCGACTACAGGAGTAAAATGGATACTGAGCCAGGAAGGTGTAGAAGAACTGATGGAAAGAAATGGAGGTGTAGTAGGGATGTAGTGCCTGATGAGAAGTACTGTGAGAAACACATGCACAGAGGGCGCCATCGTTCAAGAAAGCCTGTGGAAGCTTCTCAAGTCACTTCTCCTCCCAGCACAAAGCTACTGAAGAATTCTGACGGCGAACTGTTGAATTCAAAGAAAAACCTTCAGATTCAAACCCCCAAAGGCCTTCAACTTATGGCGCAACCCTCCAATAATGGTAGTGTCAGCCACAGTCGAACCACCTTCTCTAGTGGCTATCATGAGTTACCTAAGAACATTAGTACAGTTGCATACACTACCAGCACACCTTCATACAGAACCGCTGTTACCCCTGCTGCTGTAGCCCCTTCCACCACTGCTGGACAAATGACCGCAACCGCCAGCACTTGCAATGACAATAACATCTGGATCGGTGTGAAAGACTATGGTTGCAACTACCATGCTGGTAATTATGGCATTATCAGAGGAAGCAGAAACAACATCAATGTTGGCAGTAATATATCGTCCGGATCAGGCTTCTCCCCGAAAAGTGTTCTTCAGGCAGGACATATGAGTAAGATCGAACCTTTGTGCCATGCATATTTCTTTTCCCTAAATATATATTACTTGATAAGGATAACTATTAACTACTGGTCCTGA

Protein Analysis

338

Amino Acids

37.25

Weight (kDa)

9.37

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 46 - 79 3.8e-10 QLQ
WRC PF08879 113 - 154 2e-21 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 435
AccIII TCCGGA 1 cut(s) 884
AciI CCGC 5 cut(s) 56, 87, 699, 750, 756
AclWI GGATC 3 cut(s) 55, 794, 895
AcsI RAATTY 2 cut(s) 505, 526
AcuI CTGAAG 3 cut(s) 521, 527, 899
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 3 cut(s) 188, 410, 661
AgsI TTSAA 5 cut(s) 447, 526, 531, 551, 569
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 6 cut(s) 70, 194, 220, 291, 464, 496
AluI AGCT 6 cut(s) 70, 194, 220, 291, 464, 496
AlwI GGATC 3 cut(s) 55, 794, 895
Aor13HI TCCGGA 1 cut(s) 884
AoxI GGCC 1 cut(s) 562
ApeKI GCWGC 5 cut(s) 166, 194, 248, 266, 713
ApoI RAATTY 2 cut(s) 505, 526
AspLEI GCGC 2 cut(s) 438, 580
AspS9I GGNCC 1 cut(s) 1011
AsuHPI GGTGA 2 cut(s) 101, 110
AvaII GGWCC 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 435
BbvI GCAGC 5 cut(s) 178, 181, 253, 260, 700
BccI CCATC 3 cut(s) 86, 356, 447
BceAI ACGGC 2 cut(s) 43, 529
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 325
BfaI CTAG 2 cut(s) 296, 627
BfmI CTRYAG 2 cut(s) 316, 717
BfoI RGCGCY 1 cut(s) 439
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 6 cut(s) 56, 167, 195, 249, 267, 714
BlsI GCNGC 6 cut(s) 57, 168, 196, 250, 268, 715
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 1 cut(s) 1011
BmgT120I GGNCC 1 cut(s) 1011
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 436
BsaWI WCCGGW 1 cut(s) 884
BsaXI ACNNNNNCTCC 6 cut(s) 115, 145, 367, 397, 466, 496
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 1013
Bse3DI GCAATG 1 cut(s) 775
BseAI TCCGGA 1 cut(s) 884
BseBI CCWGG 1 cut(s) 342
BseGI GGATG 3 cut(s) 43, 160, 394
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 2 cut(s) 161, 327
BseNI ACTGG 1 cut(s) 1013
BseRI GAGGAG 4 cut(s) 136, 139, 142, 471
BseXI GCAGC 5 cut(s) 178, 181, 253, 260, 700
BseYI CCCAGC 3 cut(s) 197, 287, 485
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 564
BshNI GGYRCC 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 2 cut(s) 252, 885
BsnI GGCC 1 cut(s) 564
Bsp13I TCCGGA 1 cut(s) 884
Bsp143I GATC 6 cut(s) 47, 63, 81, 786, 887, 936
BspACI CCGC 5 cut(s) 56, 87, 699, 750, 756
BspANI GGCC 1 cut(s) 564
BspCNI CTCAG 2 cut(s) 160, 328
BspEI TCCGGA 1 cut(s) 884
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 1 cut(s) 437
BspPI GGATC 3 cut(s) 55, 794, 895
BspT107I GGYRCC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 775
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 1 cut(s) 1013
BssAI RCCGGY 1 cut(s) 251
BssMI GATC 6 cut(s) 47, 63, 81, 786, 887, 936
BssNI GRCGYC 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 5 cut(s) 142, 413, 522, 611, 664
Bst6I CTCTTC 2 cut(s) 113, 233
BstACI GRCGYC 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 207, 760
BstDEI CTNAG 3 cut(s) 147, 336, 648
BstF5I GGATG 3 cut(s) 43, 160, 394
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 2 cut(s) 438, 580
BstKTI GATC 6 cut(s) 50, 66, 84, 789, 890, 939
BstMBI GATC 6 cut(s) 47, 63, 81, 786, 887, 936
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 172, 433, 719
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 1 cut(s) 340
BstSFI CTRYAG 2 cut(s) 316, 717
BstV1I GCAGC 5 cut(s) 178, 181, 253, 260, 700
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 1 cut(s) 564
BtsCI GGATG 3 cut(s) 43, 160, 394
BtsI GCAGTG 1 cut(s) 732
BtsIMutI CAGTG 1 cut(s) 732
Cac8I GCNNGC 2 cut(s) 207, 760
CciI TCATGA 1 cut(s) 637
CfoI GCGC 2 cut(s) 438, 580
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 1 cut(s) 1011
Csp6I GTAC 3 cut(s) 187, 409, 660
CviAII CATG 5 cut(s) 32, 424, 638, 818, 953
CviQI GTAC 3 cut(s) 187, 409, 660
DdeI CTNAG 3 cut(s) 147, 336, 648
DinI GGCGCC 1 cut(s) 437
DpnI GATC 6 cut(s) 49, 65, 83, 788, 889, 938
DpnII GATC 6 cut(s) 47, 63, 81, 786, 887, 936
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
Eam1104I CTCTTC 2 cut(s) 113, 233
EarI CTCTTC 2 cut(s) 113, 233
Eco147I AGGCCT 1 cut(s) 564
Eco47I GGWCC 1 cut(s) 1011
Eco57I CTGAAG 3 cut(s) 521, 527, 899
EcoRI GAATTC 2 cut(s) 505, 526
EcoRII CCWGG 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 958
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
FaeI CATG 5 cut(s) 35, 427, 641, 821, 956
FatI CATG 5 cut(s) 31, 423, 637, 817, 952
FauNDI CATATG 1 cut(s) 927
Fnu4HI GCNGC 6 cut(s) 56, 167, 195, 249, 267, 714
FokI GGATG 3 cut(s) 50, 167, 401
Fsp4HI GCNGC 6 cut(s) 56, 167, 195, 249, 267, 714
FspBI CTAG 2 cut(s) 296, 627
GlaI GCGC 2 cut(s) 437, 579
GluI GCNGC 6 cut(s) 56, 167, 195, 249, 267, 714
GsaI CCCAGC 3 cut(s) 201, 291, 489
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 1 cut(s) 564
HapII CCGG 2 cut(s) 252, 885
HhaI GCGC 2 cut(s) 438, 580
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 5 cut(s) 35, 427, 641, 821, 956
Hin6I GCGC 2 cut(s) 436, 578
HinP1I GCGC 2 cut(s) 436, 578
HindIII AAGCTT 1 cut(s) 462
HinfI GANTC 1 cut(s) 547
HpaII CCGG 2 cut(s) 252, 885
HphI GGTGA 2 cut(s) 101, 110
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 3 cut(s) 511, 546, 842
Hpy188III TCNNGA 6 cut(s) 19, 447, 638, 784, 885, 1014
Hpy8I GTNNAC 1 cut(s) 138
Hpy99I CGWCG 2 cut(s) 55, 64
HpyAV CCTTC 8 cut(s) 16, 38, 339, 551, 575, 631, 696, 735
HpyCH4III ACNGT 5 cut(s) 142, 413, 522, 611, 664
HpyCH4V TGCA 5 cut(s) 427, 668, 768, 810, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 433, 719
HpyF3I CTNAG 3 cut(s) 147, 336, 648
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 5 cut(s) 35, 427, 641, 821, 956
HspAI GCGC 2 cut(s) 436, 578
KasI GGCGCC 1 cut(s) 435
Kpn2I TCCGGA 1 cut(s) 884
Kzo9I GATC 6 cut(s) 47, 63, 81, 786, 887, 936
LmnI GCTCC 2 cut(s) 163, 214
Lsp1109I GCAGC 5 cut(s) 178, 181, 253, 260, 700
MaeI CTAG 2 cut(s) 296, 627
MaeIII GTNAC 3 cut(s) 472, 642, 703
MalI GATC 6 cut(s) 49, 65, 83, 788, 889, 938
MboI GATC 6 cut(s) 47, 63, 81, 786, 887, 936
MboII GAAGA 5 cut(s) 130, 250, 366, 514, 905
MluCI AATT 3 cut(s) 505, 526, 826
Mly113I GGCGCC 1 cut(s) 436
MmeI TCCRAC 2 cut(s) 132, 141
Mph1103I ATGCAT 1 cut(s) 958
MroI TCCGGA 1 cut(s) 884
MseI TTAA 2 cut(s) 183, 1002
MslI CAYNNNNRTG 1 cut(s) 36
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 2 cut(s) 252, 885
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 3 cut(s) 172, 433, 719
NarI GGCGCC 1 cut(s) 436
NdeI CATATG 1 cut(s) 927
NdeII GATC 6 cut(s) 47, 63, 81, 786, 887, 936
NlaIII CATG 5 cut(s) 35, 427, 641, 821, 956
NlaIV GGNNCC 1 cut(s) 437
NmuCI GTSAC 1 cut(s) 472
NsiI ATGCAT 1 cut(s) 958
NspI RCATGY 1 cut(s) 427
PagI TCATGA 1 cut(s) 637
PceI AGGCCT 1 cut(s) 564
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 59
PfeI GAWTC 1 cut(s) 547
PkrI GCNGC 6 cut(s) 57, 168, 196, 250, 268, 715
Ple19I CGATCG 1 cut(s) 84
PluTI GGCGCC 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 3 cut(s) 197, 287, 485
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 437
PspPI GGNCC 1 cut(s) 1011
PsrI GAACNNNNNNTAC 2 cut(s) 688, 720
PvuI CGATCG 1 cut(s) 84
RsaI GTAC 3 cut(s) 188, 410, 661
RsaNI GTAC 3 cut(s) 187, 409, 660
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 2 cut(s) 183, 1002
SatI GCNGC 6 cut(s) 56, 167, 195, 249, 267, 714
Sau3AI GATC 6 cut(s) 47, 63, 81, 786, 887, 936
Sau96I GGNCC 1 cut(s) 1011
ScaI AGTACT 1 cut(s) 410
ScrFI CCNGG 1 cut(s) 342
SfcI CTRYAG 2 cut(s) 316, 717
SfoI GGCGCC 1 cut(s) 437
SinI GGWCC 1 cut(s) 1011
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 3 cut(s) 505, 526, 826
SseBI AGGCCT 1 cut(s) 564
SsiI CCGC 5 cut(s) 56, 87, 699, 750, 756
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 2 cut(s) 296, 627
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 1 cut(s) 340
TaaI ACNGT 5 cut(s) 142, 413, 522, 611, 664
TaqI TCGA 4 cut(s) 62, 312, 613, 939
TaqII GACCGA 1 cut(s) 62
TasI AATT 3 cut(s) 505, 526, 826
TatI WGTACW 3 cut(s) 186, 408, 659
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 1 cut(s) 547
Tru1I TTAA 2 cut(s) 183, 1002
Tru9I TTAA 2 cut(s) 183, 1002
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 5 cut(s) 166, 194, 248, 266, 713
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 3 cut(s) 17, 57, 678
TspRI CASTG 1 cut(s) 739
VpaK11BI GGWCC 1 cut(s) 1011
XapI RAATTY 2 cut(s) 505, 526
XceI RCATGY 1 cut(s) 427
XspI CTAG 2 cut(s) 296, 627
ZrmI AGTACT 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.