Rroxscaffold_4G00304440

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012482
Physical Location & Seq
Reverse (-)
25091566 .. 25094027
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304440.1

Sequence Viewer

Length: 1338 bp
ATGAACGACGCCCAAGTCGTGAAGGTCAAGCATGAGGATGATGAAGGATCGTCGCCGCCGTCGATCAAGCTCGGTCTTGCGATCGGCGGTGATGGTGGTGACTGTGAAGTAGCGGGGAAGAGGAGGAGGAAGAGTGAGTTTACGGTGGCTCAGTTGGATGAGTTGGAGCAGCAGAGGCTTGTTTACAAGTACATAGCTGCTGGGTTGCCGGCTCCTTTTCAGCTTGTGCTTCCCATTTGGAAGAGTGTGGCTGCCGGTTTTTCTAATGCTGCTTTCTACCAGCAATATCCCAGCTTTCTAGGATATGGGGGTTTCGACTACAGGAGTAAAATGGATACTGAGCCAGGAAGGTGTAGAAGAACTGATGGAAAGAAATGGAGGTGTAGTAGGGATGTAGTGCCTGATGAGAAGTACTGTGAGAAACACATGCACAGAGGGCGTCATCGTTCAAGAAAGCCTGTGGAAACTTCTCAAGTCACTTCTCCTCCCAGCACAAAGCTACCGAAGAATTCAGACGGCGAACTGTTGAATTCAAAGAAAAACCTTCAGATTCAAACCCCCAAAGGCCTTCAACTTATGGCGCAACCCTCCAATAATGGTAGTGTCAGCCACAGTGGAACCACCTTCTCTAGTGGCTATCATGAGTTACCTAAGAACATTAGTACGGTTGCATTCACTACCAGCACACCTCCATACAGAACCGCTGTTACCCCTGCTGCTGTAGCCCCTTCCACCACTGCTGGACAAATGACCACAACCGCCAGCACTTGCAATGACAATAACATCTGGATCGGTGTGAAAGACTATGGTTGCAACTACCATGCTGGTAGTTATGGCATTATCAGAGGAAGCAGAAACAACATCAATGTTGGCAGTAATATATCTCCCGGATCAGGCTTCTCCCCCAAAAGTGTTCTTCAGGCAGGACATATGATTCTTGTAAATTGCAATGGCTTATACTTTGACAACAGACGTGGCGAAGAACTTGAACCAGGCAGGTGTAGGAGGACAGATGGAAAGAAGTGGCGGTGCAAAAGGACTGTTCTTCCTGATCAGAAGTACTGTGACCGGCATATCCACAGAGGAGTAAAGCAAATGAGAACCTCTGGCCCTGTTTCTGCTGCTTCTACCTCGGCCACTGCAATGAATTCTACTTGGCCCTCTCAGACCACAGGCATAGCTGGAGAAACTGATTGTGCAATCCCTAATACAAATCTCACCATGTCCATCCCAGCAAGATCACCACTGATGCAAAATGATGAAAAAAGTGATTCCACTAGCGACAGTGACGCAACCATTACAGACACCAGCCTTGCTGCTTATGAACAGCTGATTTAG

Protein Analysis

445

Amino Acids

48.57

Weight (kDa)

9.17

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 47 - 80 3.7e-10 QLQ
WRC PF08879 114 - 155 3e-21 WRC
WRC PF08879 330 - 368 3.8e-19 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 987
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 987
AciI CCGC 6 cut(s) 56, 87, 113, 702, 759, 1027
AclWI GGATC 3 cut(s) 55, 797, 898
AcoI YGGCCR 1 cut(s) 1134
AcsI RAATTY 3 cut(s) 508, 529, 1147
AcuI CTGAAG 2 cut(s) 530, 902
AcyI GRCGYC 2 cut(s) 9, 439
AfaI GTAC 4 cut(s) 191, 413, 664, 1061
AfiI CCNNNNNNNGG 1 cut(s) 893
AgsI TTSAA 6 cut(s) 450, 529, 534, 554, 572, 989
AjiI CACGTC 1 cut(s) 974
AjnI CCWGG 2 cut(s) 343, 991
AluBI AGCT 7 cut(s) 70, 197, 223, 294, 499, 1181, 1330
AluI AGCT 7 cut(s) 70, 197, 223, 294, 499, 1181, 1330
AlwI GGATC 3 cut(s) 55, 797, 898
AoxI GGCC 4 cut(s) 565, 1108, 1134, 1157
ApeKI GCWGC 7 cut(s) 169, 197, 251, 269, 716, 1121, 1316
ApoI RAATTY 3 cut(s) 508, 529, 1147
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 2 cut(s) 1109, 1158
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 4 cut(s) 101, 110, 1210, 1233
BbvI GCAGC 7 cut(s) 181, 184, 238, 256, 703, 1108, 1303
BccI CCATC 4 cut(s) 86, 359, 1007, 1235
BceAI ACGGC 2 cut(s) 43, 532
BciT130I CCWGG 2 cut(s) 345, 993
BciVI GTATCC 1 cut(s) 328
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 888
BfaI CTAG 3 cut(s) 299, 630, 1278
BfmI CTRYAG 2 cut(s) 319, 720
BfuAI ACCTGC 1 cut(s) 987
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 8 cut(s) 56, 170, 198, 252, 270, 717, 1122, 1317
BlsI GCNGC 8 cut(s) 57, 171, 199, 253, 271, 718, 1123, 1318
BmcAI AGTACT 2 cut(s) 413, 1061
Bme1390I CCNGG 3 cut(s) 345, 888, 993
BmgBI CACGTC 1 cut(s) 974
BmgT120I GGNCC 2 cut(s) 1109, 1158
BmiI GGNNCC 2 cut(s) 213, 619
BmrFI CCNGG 3 cut(s) 345, 888, 993
BmsI GCATC 1 cut(s) 1239
BpmI CTGGAG 1 cut(s) 1203
BpuEI CTTGAG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 888
BsaHI GRCGYC 2 cut(s) 9, 439
BsaJI CCNNGG 1 cut(s) 1131
BsaXI ACNNNNNCTCC 6 cut(s) 118, 148, 370, 400, 469, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 893
Bse118I RCCGGY 3 cut(s) 208, 254, 1068
Bse3DI GCAATG 3 cut(s) 778, 955, 1149
BseBI CCWGG 2 cut(s) 345, 993
BseDI CCNNGG 1 cut(s) 1131
BseGI GGATG 4 cut(s) 43, 163, 397, 1227
BseLI CCNNNNNNNGG 1 cut(s) 893
BseMI GCAATG 3 cut(s) 778, 955, 1149
BseMII CTCAG 3 cut(s) 164, 330, 1178
BseRI GAGGAG 4 cut(s) 136, 139, 474, 1098
BseXI GCAGC 7 cut(s) 181, 184, 238, 256, 703, 1108, 1303
BseYI CCCAGC 4 cut(s) 200, 290, 488, 1231
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 567, 1110, 1136, 1159
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 4 cut(s) 209, 255, 888, 1069
BslI CCNNNNNNNGG 1 cut(s) 893
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 4 cut(s) 567, 1110, 1136, 1159
Bsp143I GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
BspACI CCGC 6 cut(s) 56, 87, 113, 702, 759, 1027
BspANI GGCC 4 cut(s) 567, 1110, 1136, 1159
BspCNI CTCAG 3 cut(s) 163, 331, 1177
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 2 cut(s) 213, 619
BspMI ACCTGC 1 cut(s) 987
BspPI GGATC 3 cut(s) 55, 797, 898
BsrDI GCAATG 3 cut(s) 778, 955, 1149
BsrFI RCCGGY 3 cut(s) 208, 254, 1068
BssAI RCCGGY 3 cut(s) 208, 254, 1068
BssECI CCNNGG 1 cut(s) 1131
BssMI GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
BssNI GRCGYC 2 cut(s) 9, 439
Bst2UI CCWGG 2 cut(s) 345, 993
Bst4CI ACNGT 9 cut(s) 104, 145, 416, 525, 614, 667, 1042, 1064, 1286
Bst6I CTCTTC 3 cut(s) 113, 125, 236
BstACI GRCGYC 2 cut(s) 9, 439
BstC8I GCNNGC 2 cut(s) 210, 763
BstDEI CTNAG 4 cut(s) 150, 339, 651, 1164
BstF5I GGATG 4 cut(s) 43, 163, 397, 1227
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 7 cut(s) 50, 66, 84, 792, 893, 1054, 1241
BstMBI GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 175, 436, 722
BstNI CCWGG 2 cut(s) 345, 993
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 343, 886, 991
BstSFI CTRYAG 2 cut(s) 319, 720
BstV1I GCAGC 7 cut(s) 181, 184, 238, 256, 703, 1108, 1303
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 4 cut(s) 567, 1110, 1136, 1159
BtrI CACGTC 1 cut(s) 974
BtsCI GGATG 4 cut(s) 43, 163, 397, 1227
BtsI GCAGTG 2 cut(s) 735, 1137
BtsIMutI CAGTG 5 cut(s) 619, 735, 1137, 1244, 1291
BveI ACCTGC 1 cut(s) 987
Cac8I GCNNGC 2 cut(s) 210, 763
CciI TCATGA 1 cut(s) 640
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 3 cut(s) 208, 254, 1068
Cfr13I GGNCC 2 cut(s) 1109, 1158
CseI GACGC 3 cut(s) 17, 428, 1298
Csp6I GTAC 4 cut(s) 190, 412, 663, 1060
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviAII CATG 5 cut(s) 32, 427, 641, 821, 1222
CviQI GTAC 4 cut(s) 190, 412, 663, 1060
DdeI CTNAG 4 cut(s) 150, 339, 651, 1164
DpnI GATC 7 cut(s) 49, 65, 83, 791, 892, 1053, 1240
DpnII GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 1 cut(s) 1134
Eam1104I CTCTTC 3 cut(s) 113, 125, 236
EarI CTCTTC 3 cut(s) 113, 125, 236
Eco147I AGGCCT 1 cut(s) 567
Eco57I CTGAAG 2 cut(s) 530, 902
EcoRI GAATTC 3 cut(s) 508, 529, 1147
EcoRII CCWGG 2 cut(s) 343, 991
FaeI CATG 5 cut(s) 35, 430, 644, 824, 1225
FatI CATG 5 cut(s) 31, 426, 640, 820, 1221
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 930
FbaI TGATCA 1 cut(s) 1051
Fnu4HI GCNGC 8 cut(s) 56, 170, 198, 252, 270, 717, 1122, 1317
FokI GGATG 4 cut(s) 50, 170, 404, 1214
Fsp4HI GCNGC 8 cut(s) 56, 170, 198, 252, 270, 717, 1122, 1317
FspBI CTAG 3 cut(s) 299, 630, 1278
GlaI GCGC 1 cut(s) 582
GluI GCNGC 8 cut(s) 56, 170, 198, 252, 270, 717, 1122, 1317
GsaI CCCAGC 4 cut(s) 204, 294, 492, 1235
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 4 cut(s) 567, 1110, 1136, 1159
HapII CCGG 4 cut(s) 209, 255, 888, 1069
HgaI GACGC 3 cut(s) 17, 428, 1298
HhaI GCGC 1 cut(s) 583
Hin1I GRCGYC 2 cut(s) 9, 439
Hin1II CATG 5 cut(s) 35, 430, 644, 824, 1225
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HinfI GANTC 3 cut(s) 550, 934, 1271
HpaII CCGG 4 cut(s) 209, 255, 888, 1069
HphI GGTGA 4 cut(s) 101, 110, 1210, 1233
Hpy166II GTNNAC 2 cut(s) 141, 184
Hpy188I TCNGA 5 cut(s) 514, 549, 845, 1056, 1167
Hpy188III TCNNGA 5 cut(s) 19, 450, 641, 787, 1049
Hpy8I GTNNAC 2 cut(s) 141, 184
Hpy99I CGWCG 3 cut(s) 11, 55, 64
HpyAV CCTTC 7 cut(s) 16, 38, 342, 554, 578, 634, 738
HpyCH4III ACNGT 9 cut(s) 104, 145, 416, 525, 614, 667, 1042, 1064, 1286
HpyCH4IV ACGT 1 cut(s) 973
HpyCH4V TGCA 9 cut(s) 430, 671, 771, 813, 948, 1032, 1142, 1199, 1252
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 436, 722
HpyF3I CTNAG 4 cut(s) 150, 339, 651, 1164
HpySE526I ACGT 1 cut(s) 973
Hsp92I GRCGYC 2 cut(s) 9, 439
Hsp92II CATG 5 cut(s) 35, 430, 644, 824, 1225
HspAI GCGC 1 cut(s) 581
KroI GCCGGC 1 cut(s) 208
KroNI GCCGGC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
LmnI GCTCC 2 cut(s) 166, 217
Lsp1109I GCAGC 7 cut(s) 181, 184, 238, 256, 703, 1108, 1303
LweI GCATC 1 cut(s) 1239
MaeI CTAG 3 cut(s) 299, 630, 1278
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 6 cut(s) 98, 475, 645, 706, 1064, 1286
MalI GATC 7 cut(s) 49, 65, 83, 791, 892, 1053, 1240
MboI GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
MboII GAAGA 8 cut(s) 130, 142, 253, 369, 517, 908, 992, 1037
MluCI AATT 4 cut(s) 508, 529, 943, 1147
MmeI TCCRAC 2 cut(s) 135, 144
MroNI GCCGGC 1 cut(s) 208
MslI CAYNNNNRTG 2 cut(s) 36, 1142
MspA1I CMGCKG 2 cut(s) 704, 1330
MspI CCGG 4 cut(s) 209, 255, 888, 1069
MspR9I CCNGG 3 cut(s) 345, 888, 993
Mva1269I GAATGC 1 cut(s) 671
MvaI CCWGG 2 cut(s) 345, 993
MwoI GCNNNNNNNGC 3 cut(s) 175, 436, 722
NaeI GCCGGC 1 cut(s) 210
NciI CCSGG 1 cut(s) 888
NdeI CATATG 1 cut(s) 930
NdeII GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
NgoMIV GCCGGC 1 cut(s) 208
NlaIII CATG 5 cut(s) 35, 430, 644, 824, 1225
NlaIV GGNNCC 2 cut(s) 213, 619
NmeAIII GCCGAG 1 cut(s) 1112
NmuCI GTSAC 4 cut(s) 98, 475, 1064, 1286
NspI RCATGY 1 cut(s) 430
PagI TCATGA 1 cut(s) 640
PaqCI CACCTGC 1 cut(s) 987
PceI AGGCCT 1 cut(s) 567
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 56, 59
PctI GAATGC 1 cut(s) 671
PdiI GCCGGC 1 cut(s) 210
PfeI GAWTC 3 cut(s) 550, 934, 1271
PfoI TCCNGGA 1 cut(s) 886
PkrI GCNGC 8 cut(s) 57, 171, 199, 253, 271, 718, 1123, 1318
Ple19I CGATCG 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 343, 991
PspFI CCCAGC 4 cut(s) 200, 290, 488, 1231
PspGI CCWGG 2 cut(s) 343, 991
PspN4I GGNNCC 2 cut(s) 213, 619
PspPI GGNCC 2 cut(s) 1109, 1158
PsrI GAACNNNNNNTAC 2 cut(s) 691, 723
PvuI CGATCG 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 1330
RsaI GTAC 4 cut(s) 191, 413, 664, 1061
RsaNI GTAC 4 cut(s) 190, 412, 663, 1060
RseI CAYNNNNRTG 2 cut(s) 36, 1142
SatI GCNGC 8 cut(s) 56, 170, 198, 252, 270, 717, 1122, 1317
Sau3AI GATC 7 cut(s) 47, 63, 81, 789, 890, 1051, 1238
Sau96I GGNCC 2 cut(s) 1109, 1158
ScaI AGTACT 2 cut(s) 413, 1061
ScrFI CCNGG 3 cut(s) 345, 888, 993
SfaNI GCATC 1 cut(s) 1239
SfcI CTRYAG 2 cut(s) 319, 720
SmiMI CAYNNNNRTG 2 cut(s) 36, 1142
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 4 cut(s) 508, 529, 943, 1147
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 6 cut(s) 56, 87, 113, 702, 759, 1027
SspMI CTAG 3 cut(s) 299, 630, 1278
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 3 cut(s) 343, 886, 991
TaaI ACNGT 9 cut(s) 104, 145, 416, 525, 614, 667, 1042, 1064, 1286
TaiI ACGT 1 cut(s) 976
TaqI TCGA 2 cut(s) 62, 315
TaqII GACCGA 1 cut(s) 62
TasI AATT 4 cut(s) 508, 529, 943, 1147
TatI WGTACW 3 cut(s) 189, 411, 1059
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 3 cut(s) 550, 934, 1271
TscAI CASTG 5 cut(s) 619, 742, 1144, 1251, 1291
TseFI GTSAC 4 cut(s) 98, 475, 1064, 1286
TseI GCWGC 7 cut(s) 169, 197, 251, 269, 716, 1121, 1316
Tsp45I GTSAC 4 cut(s) 98, 475, 1064, 1286
TspDTI ATGAA 5 cut(s) 17, 57, 1160, 1275, 1338
TspRI CASTG 5 cut(s) 619, 742, 1144, 1251, 1291
XapI RAATTY 3 cut(s) 508, 529, 1147
XceI RCATGY 1 cut(s) 430
XspI CTAG 3 cut(s) 299, 630, 1278
ZrmI AGTACT 2 cut(s) 413, 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.