Rh1AG229300

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0012482
Physical Location & Seq
Forward (+)
43579746 .. 43581873
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG229300.1

Sequence Viewer

Length: 1335 bp
ATGGACGACACCCAAGTCGTGAAGGTCAAGCGTGAGGATGATGAAGGATCGTCGCCGCCGTCGATCAAGCTCGGTCTTTCGATCGGCGGTGATGGTGGTGATTGTGAGGTAGTGGGGAAGAGGAGGAGGAGTGAGTTTACGGTGGCTCAGTTGGATGAGTTGGAGCAGCAGAGGCTTGTTTATAAGTACATAGCTGCTGGGTTGCCTGCTCCTTTTCAGCTTGTGCTTCCCATTTGGAAGAGTGTGGCTGCCGGTTTTTCTAATGCTGCTTTCTACCAGCAATATCCCAGCTTTCTAGGATATGGGGGTTTCGACTACAGGAGTAAAATGGATACTGAGCCAGGAAGGTGTAGAAGAACTGATGGAAAGAAATGGAGGTGTAGTAGGGATGTAGTGCCTGATGAGAAGTACTGTGAGAAACACATGCACAGAGGGCGCCATCGTTCAAGAAAGCCTGTGGAAACTTCTCAAGTCACTTCTCCTCCCAGCACAAAGCTACTGAAGAATTCAGATGGCGAACTGTTGAATTCAAAGAAAAACCTTCAGATTCAAACCCCCAAAGGCCTTCAACTTATGGCGCAACCCTCCAATAATGGTAGTGTCAGCCACAGTCGAACCACCTTCTCTAGTGGCTATCATGAGTTACCTAAGAACATTAGTACAGTTGCACCCACTACCAGCACACCTTCATACAGAACCGCTATTACCCCTGCTGCTGTAGCCCCTTCCACCACTGCTGGACAAGTGATCGCAACCGCCAGCACTTGCAATGACCATAACATCTGGATCGGTGTGAAAGACTACGGTTGCAACTACCATGCTAGTAATTATGGCATTATCAGAGGAAGCAGAAACAACATCAATGTTGGCAGTAATATATCGTCCGGATCAGGCTTCTCCCCAAAAAGTGTTCTTCAGGCAGGACATATGATTCTTGTAAATTGCAATGGCTTATACTTTGACAACAGACATGGCGAAGAACTTGAACCAGGCAGGTGTAGGAGGACAGATGGAAAGAAGTGGCGGTGCAAAAGGGAAGTTCTTCCTGATCAGAAGTACTGTGACCGGCATATCCACAGAGGAGTAAAGCAAATGAGAACCTCTGGCCCTGTTACTGCTGCTTCTACCTCGGCCACTGCAATGAATTCTACTCGGCCCTCTCAGACCACAGGCATAGCTGGAGAAACTGATTGTGCATTCCCTAATACAAATCTCACCATGTCCATCCCAGCAAGATCACCACTGATGCAAAATGATGAAAAAAGTGATTCCACCAGCGACAGTGACGCAACCATTACAGACACCAGCCTTGCTGCTTATGAACAGCTGATTTAG

Protein Analysis

444

Amino Acids

48.65

Weight (kDa)

9.12

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 46 - 79 3.7e-10 QLQ
WRC PF08879 113 - 154 3e-21 WRC
WRC PF08879 329 - 367 3.8e-19 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
AarI CACCTGC 1 cut(s) 984
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 984
AccB1I GGYRCC 1 cut(s) 435
AccIII TCCGGA 1 cut(s) 884
AciI CCGC 5 cut(s) 56, 87, 699, 756, 1024
AclWI GGATC 3 cut(s) 55, 794, 895
AcoI YGGCCR 1 cut(s) 1131
AcsI RAATTY 3 cut(s) 505, 526, 1144
AcuI CTGAAG 3 cut(s) 521, 527, 899
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 4 cut(s) 188, 410, 661, 1058
AgsI TTSAA 6 cut(s) 447, 526, 531, 551, 569, 986
AjnI CCWGG 2 cut(s) 340, 988
AluBI AGCT 7 cut(s) 70, 194, 220, 291, 496, 1178, 1327
AluI AGCT 7 cut(s) 70, 194, 220, 291, 496, 1178, 1327
AlwI GGATC 3 cut(s) 55, 794, 895
Aor13HI TCCGGA 1 cut(s) 884
AoxI GGCC 4 cut(s) 562, 1105, 1131, 1154
ApeKI GCWGC 7 cut(s) 166, 194, 248, 266, 713, 1118, 1313
ApoI RAATTY 3 cut(s) 505, 526, 1144
Asp700I GAANNNNTTC 1 cut(s) 1041
AspLEI GCGC 2 cut(s) 438, 580
AspS9I GGNCC 2 cut(s) 1106, 1155
AsuHPI GGTGA 4 cut(s) 101, 110, 1207, 1230
BanI GGYRCC 1 cut(s) 435
BbvI GCAGC 7 cut(s) 178, 181, 235, 253, 700, 1105, 1300
BccI CCATC 6 cut(s) 86, 356, 447, 506, 1004, 1232
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 2 cut(s) 342, 990
BciVI GTATCC 1 cut(s) 325
BclI TGATCA 1 cut(s) 1048
BfaI CTAG 3 cut(s) 296, 627, 822
BfmI CTRYAG 2 cut(s) 316, 717
BfoI RGCGCY 1 cut(s) 439
BfuAI ACCTGC 1 cut(s) 984
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 8 cut(s) 56, 167, 195, 249, 267, 714, 1119, 1314
BlsI GCNGC 8 cut(s) 57, 168, 196, 250, 268, 715, 1120, 1315
BmcAI AGTACT 2 cut(s) 410, 1058
Bme1390I CCNGG 2 cut(s) 342, 990
BmgT120I GGNCC 2 cut(s) 1106, 1155
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 2 cut(s) 342, 990
BmsI GCATC 1 cut(s) 1236
BpmI CTGGAG 1 cut(s) 1200
BpuEI CTTGAG 1 cut(s) 453
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 1128
BsaWI WCCGGW 1 cut(s) 884
BsaXI ACNNNNNCTCC 6 cut(s) 115, 145, 367, 397, 466, 496
Bse118I RCCGGY 2 cut(s) 251, 1065
Bse3DI GCAATG 3 cut(s) 775, 952, 1146
BseAI TCCGGA 1 cut(s) 884
BseBI CCWGG 2 cut(s) 342, 990
BseDI CCNNGG 1 cut(s) 1128
BseGI GGATG 4 cut(s) 43, 160, 394, 1224
BseMI GCAATG 3 cut(s) 775, 952, 1146
BseMII CTCAG 3 cut(s) 161, 327, 1175
BseRI GAGGAG 5 cut(s) 136, 139, 142, 471, 1095
BseXI GCAGC 7 cut(s) 178, 181, 235, 253, 700, 1105, 1300
BseYI CCCAGC 4 cut(s) 197, 287, 485, 1228
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 564, 1107, 1133, 1156
BshNI GGYRCC 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 84
BsiSI CCGG 3 cut(s) 252, 885, 1066
BsmI GAATGC 1 cut(s) 1196
BsnI GGCC 4 cut(s) 564, 1107, 1133, 1156
Bsp13I TCCGGA 1 cut(s) 884
Bsp143I GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
BspACI CCGC 5 cut(s) 56, 87, 699, 756, 1024
BspANI GGCC 4 cut(s) 564, 1107, 1133, 1156
BspCNI CTCAG 3 cut(s) 160, 328, 1174
BspEI TCCGGA 1 cut(s) 884
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 1 cut(s) 437
BspMI ACCTGC 1 cut(s) 984
BspPI GGATC 3 cut(s) 55, 794, 895
BspT107I GGYRCC 1 cut(s) 435
BsrDI GCAATG 3 cut(s) 775, 952, 1146
BsrFI RCCGGY 2 cut(s) 251, 1065
BssAI RCCGGY 2 cut(s) 251, 1065
BssECI CCNNGG 1 cut(s) 1128
BssMI GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
BssNI GRCGYC 1 cut(s) 436
Bst2UI CCWGG 2 cut(s) 342, 990
Bst4CI ACNGT 8 cut(s) 142, 413, 522, 611, 664, 806, 1061, 1283
Bst6I CTCTTC 2 cut(s) 113, 233
BstACI GRCGYC 1 cut(s) 436
BstC8I GCNNGC 2 cut(s) 207, 760
BstDEI CTNAG 4 cut(s) 147, 336, 648, 1161
BstF5I GGATG 4 cut(s) 43, 160, 394, 1224
BstH2I RGCGCY 1 cut(s) 439
BstHHI GCGC 2 cut(s) 438, 580
BstKTI GATC 8 cut(s) 50, 66, 84, 750, 789, 890, 1051, 1238
BstMBI GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 172, 433, 719
BstNI CCWGG 2 cut(s) 342, 990
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 2 cut(s) 340, 988
BstSFI CTRYAG 2 cut(s) 316, 717
BstV1I GCAGC 7 cut(s) 178, 181, 235, 253, 700, 1105, 1300
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 4 cut(s) 564, 1107, 1133, 1156
BtsCI GGATG 4 cut(s) 43, 160, 394, 1224
BtsI GCAGTG 2 cut(s) 732, 1134
BtsIMutI CAGTG 4 cut(s) 732, 1134, 1241, 1288
BveI ACCTGC 1 cut(s) 984
Cac8I GCNNGC 2 cut(s) 207, 760
CciI TCATGA 1 cut(s) 637
CfoI GCGC 2 cut(s) 438, 580
Cfr10I RCCGGY 2 cut(s) 251, 1065
Cfr13I GGNCC 2 cut(s) 1106, 1155
CseI GACGC 1 cut(s) 1295
Csp6I GTAC 4 cut(s) 187, 409, 660, 1057
CviAII CATG 5 cut(s) 424, 638, 818, 971, 1219
CviQI GTAC 4 cut(s) 187, 409, 660, 1057
DdeI CTNAG 4 cut(s) 147, 336, 648, 1161
DinI GGCGCC 1 cut(s) 437
DpnI GATC 8 cut(s) 49, 65, 83, 749, 788, 889, 1050, 1237
DpnII GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 1 cut(s) 1131
Eam1104I CTCTTC 2 cut(s) 113, 233
EarI CTCTTC 2 cut(s) 113, 233
Eco147I AGGCCT 1 cut(s) 564
Eco57I CTGAAG 3 cut(s) 521, 527, 899
EcoRI GAATTC 3 cut(s) 505, 526, 1144
EcoRII CCWGG 2 cut(s) 340, 988
EgeI GGCGCC 1 cut(s) 437
EheI GGCGCC 1 cut(s) 437
FaeI CATG 5 cut(s) 427, 641, 821, 974, 1222
FatI CATG 5 cut(s) 423, 637, 817, 970, 1218
FauNDI CATATG 1 cut(s) 927
FbaI TGATCA 1 cut(s) 1048
Fnu4HI GCNGC 8 cut(s) 56, 167, 195, 249, 267, 714, 1119, 1314
FokI GGATG 4 cut(s) 50, 167, 401, 1211
Fsp4HI GCNGC 8 cut(s) 56, 167, 195, 249, 267, 714, 1119, 1314
FspBI CTAG 3 cut(s) 296, 627, 822
GlaI GCGC 2 cut(s) 437, 579
GluI GCNGC 8 cut(s) 56, 167, 195, 249, 267, 714, 1119, 1314
GsaI CCCAGC 4 cut(s) 201, 291, 489, 1232
GsuI CTGGAG 1 cut(s) 1200
HaeII RGCGCY 1 cut(s) 439
HaeIII GGCC 4 cut(s) 564, 1107, 1133, 1156
HapII CCGG 3 cut(s) 252, 885, 1066
HgaI GACGC 1 cut(s) 1295
HhaI GCGC 2 cut(s) 438, 580
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 5 cut(s) 427, 641, 821, 974, 1222
Hin6I GCGC 2 cut(s) 436, 578
HinP1I GCGC 2 cut(s) 436, 578
HinfI GANTC 3 cut(s) 547, 931, 1268
HpaII CCGG 3 cut(s) 252, 885, 1066
HphI GGTGA 4 cut(s) 101, 110, 1207, 1230
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 5 cut(s) 511, 546, 842, 1053, 1164
Hpy188III TCNNGA 6 cut(s) 19, 447, 638, 784, 885, 1046
Hpy8I GTNNAC 1 cut(s) 138
Hpy99I CGWCG 2 cut(s) 55, 64
HpyAV CCTTC 8 cut(s) 16, 38, 339, 551, 575, 631, 696, 735
HpyCH4III ACNGT 8 cut(s) 142, 413, 522, 611, 664, 806, 1061, 1283
HpyCH4V TGCA 9 cut(s) 427, 668, 768, 810, 945, 1029, 1139, 1196, 1249
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 433, 719
HpyF3I CTNAG 4 cut(s) 147, 336, 648, 1161
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 5 cut(s) 427, 641, 821, 974, 1222
HspAI GCGC 2 cut(s) 436, 578
KasI GGCGCC 1 cut(s) 435
Kpn2I TCCGGA 1 cut(s) 884
Ksp22I TGATCA 1 cut(s) 1048
Kzo9I GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
LmnI GCTCC 2 cut(s) 163, 214
Lsp1109I GCAGC 7 cut(s) 178, 181, 235, 253, 700, 1105, 1300
LweI GCATC 1 cut(s) 1236
MaeI CTAG 3 cut(s) 296, 627, 822
MaeIII GTNAC 5 cut(s) 472, 642, 1061, 1111, 1283
MalI GATC 8 cut(s) 49, 65, 83, 749, 788, 889, 1050, 1237
MboI GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
MboII GAAGA 7 cut(s) 130, 250, 366, 514, 905, 989, 1034
MluCI AATT 5 cut(s) 505, 526, 826, 940, 1144
Mly113I GGCGCC 1 cut(s) 436
MmeI TCCRAC 2 cut(s) 132, 141
MroI TCCGGA 1 cut(s) 884
MroXI GAANNNNTTC 1 cut(s) 1041
MslI CAYNNNNRTG 1 cut(s) 1139
MspA1I CMGCKG 1 cut(s) 1327
MspI CCGG 3 cut(s) 252, 885, 1066
MspR9I CCNGG 2 cut(s) 342, 990
Mva1269I GAATGC 1 cut(s) 1196
MvaI CCWGG 2 cut(s) 342, 990
MwoI GCNNNNNNNGC 3 cut(s) 172, 433, 719
NarI GGCGCC 1 cut(s) 436
NdeI CATATG 1 cut(s) 927
NdeII GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
NlaIII CATG 5 cut(s) 427, 641, 821, 974, 1222
NlaIV GGNNCC 1 cut(s) 437
NmeAIII GCCGAG 2 cut(s) 1109, 1132
NmuCI GTSAC 3 cut(s) 472, 1061, 1283
NspI RCATGY 1 cut(s) 427
PagI TCATGA 1 cut(s) 637
PaqCI CACCTGC 1 cut(s) 984
PceI AGGCCT 1 cut(s) 564
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 59
PctI GAATGC 1 cut(s) 1196
PdmI GAANNNNTTC 1 cut(s) 1041
PfeI GAWTC 3 cut(s) 547, 931, 1268
PkrI GCNGC 8 cut(s) 57, 168, 196, 250, 268, 715, 1120, 1315
Ple19I CGATCG 1 cut(s) 84
PluTI GGCGCC 1 cut(s) 439
PsiI TTATAA 1 cut(s) 183
Psp6I CCWGG 2 cut(s) 340, 988
PspFI CCCAGC 4 cut(s) 197, 287, 485, 1228
PspGI CCWGG 2 cut(s) 340, 988
PspN4I GGNNCC 1 cut(s) 437
PspPI GGNCC 2 cut(s) 1106, 1155
PsrI GAACNNNNNNTAC 2 cut(s) 688, 720
PvuI CGATCG 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 1327
RsaI GTAC 4 cut(s) 188, 410, 661, 1058
RsaNI GTAC 4 cut(s) 187, 409, 660, 1057
RseI CAYNNNNRTG 1 cut(s) 1139
SatI GCNGC 8 cut(s) 56, 167, 195, 249, 267, 714, 1119, 1314
Sau3AI GATC 8 cut(s) 47, 63, 81, 747, 786, 887, 1048, 1235
Sau96I GGNCC 2 cut(s) 1106, 1155
ScaI AGTACT 2 cut(s) 410, 1058
ScrFI CCNGG 2 cut(s) 342, 990
SfaNI GCATC 1 cut(s) 1236
SfcI CTRYAG 2 cut(s) 316, 717
SfoI GGCGCC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 1139
SmlI CTYRAG 1 cut(s) 468
SmoI CTYRAG 1 cut(s) 468
Sse9I AATT 5 cut(s) 505, 526, 826, 940, 1144
SseBI AGGCCT 1 cut(s) 564
SsiI CCGC 5 cut(s) 56, 87, 699, 756, 1024
SspDI GGCGCC 1 cut(s) 435
SspMI CTAG 3 cut(s) 296, 627, 822
StuI AGGCCT 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 340, 988
TaaI ACNGT 8 cut(s) 142, 413, 522, 611, 664, 806, 1061, 1283
TaqI TCGA 4 cut(s) 62, 80, 312, 613
TaqII GACCGA 1 cut(s) 62
TasI AATT 5 cut(s) 505, 526, 826, 940, 1144
TatI WGTACW 4 cut(s) 186, 408, 659, 1056
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 3 cut(s) 547, 931, 1268
TscAI CASTG 4 cut(s) 739, 1141, 1248, 1288
TseFI GTSAC 3 cut(s) 472, 1061, 1283
TseI GCWGC 7 cut(s) 166, 194, 248, 266, 713, 1118, 1313
Tsp45I GTSAC 3 cut(s) 472, 1061, 1283
TspDTI ATGAA 5 cut(s) 57, 678, 1157, 1272, 1335
TspRI CASTG 4 cut(s) 739, 1141, 1248, 1288
XapI RAATTY 3 cut(s) 505, 526, 1144
XceI RCATGY 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 1041
XspI CTAG 3 cut(s) 296, 627, 822
ZrmI AGTACT 2 cut(s) 410, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.