Rorug01G0215000

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012482
Physical Location & Seq
Forward (+)
30546717 .. 30696317
149601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0215000.1

Sequence Viewer

Length: 870 bp
ATGCAATTAGGAGCTGTCCCTAACTTGGGAGATTACATTCCTTGCATTGCTCCACTCGATCTCCAACGATTGACTAAACGAATGAAGGCTGTTCACAGGGTCTTTGATGACTTTTTGGAGAAGATTGTGGAGGAGCATCTTCAATCTAGGGATCATGGAGAAAGAACCAAGGACTTTGTTGATGTCATGCTGGGCTTCATGGGGTCTGAAGAATCTGCGTACCAAATCGGACGCTCAAACATCAAAGCCATCATATTGGATCTATTTGTTGCTGGCACAGACACAACTTCAGCCACAATGGAATGGGCAATGGCTGAGCTGCTTCGCAACCCAGAAATACTTTCAAAAGCCCAAGCAGAACTCGGTCAAGTGATTGGAAAAGGAAAACTAGTTGAGGAATCAGACATTGCACTTCTCCCTTACTTACAAGCAATAATAAAAGAAACATTTCGGTTACACCCAACAGTTCCATTGCTACTTCCTCGGAAAGCAAAAACAAACATAGAAATTGGCGGGTATGTAATCCCAAAGGGTGCTCAAGTTTTAATCAATGTTTGGGCCATAGGCAGAGACCCCATCACTTGGGAAAACCCAAACTTGTTTATGCCTGAGAGGTTCCTGGGATTTGACAACCAAATTGATGTTACGGGGAAAAACTTTGAGCTTATTCTGTTTGGTGGTGGAAGGAGAATATGCCCCGAACTTCCATTGGCAGTAAGAATGTTGCACTTGATGTTGGGTTCACTAATTAACTGCTTTGATTGGAAGCTTGAAGATGGAGTTGTACCCGAGACTATGAACATGGAAGATAAGTTTGGCATCACCTTACAAATGGCTCAGCCTCTGAGAGCTGTGCCCAAGAAGTCATAG

Protein Analysis

289

Amino Acids

32.45

Weight (kDa)

5.65

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 6 - 272 2.9e-75 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 582
AciI CCGC 1 cut(s) 513
AclWI GGATC 2 cut(s) 159, 267
AcuI CTGAAG 2 cut(s) 228, 273
AfaI GTAC 2 cut(s) 221, 786
AfiI CCNNNNNNNGG 3 cut(s) 25, 26, 582
AgsI TTSAA 3 cut(s) 143, 345, 773
AhlI ACTAGT 1 cut(s) 388
AjnI CCWGG 1 cut(s) 618
AjuI GAANNNNNNNTTGG 2 cut(s) 798, 830
AluBI AGCT 5 cut(s) 14, 319, 664, 769, 851
AluI AGCT 5 cut(s) 14, 319, 664, 769, 851
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 2 cut(s) 564, 785
AlwI GGATC 2 cut(s) 159, 267
AlwNI CAGNNNCTG 1 cut(s) 844
Ama87I CYCGRG 1 cut(s) 788
AoxI GGCC 1 cut(s) 558
ApeKI GCWGC 1 cut(s) 319
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 814
AvaI CYCGRG 1 cut(s) 788
BaeGI GKGCMC 1 cut(s) 858
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 306
BccI CCATC 3 cut(s) 257, 584, 770
BciT130I CCWGG 1 cut(s) 620
BcoDI GTCTC 2 cut(s) 564, 785
BcuI ACTAGT 1 cut(s) 388
BfaI CTAG 2 cut(s) 147, 389
BisI GCNGC 1 cut(s) 320
BlpI GCTNAGC 2 cut(s) 315, 837
BlsI GCNGC 1 cut(s) 321
Bme1390I CCNGG 1 cut(s) 620
BmeT110I CYCGRG 1 cut(s) 788
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 1 cut(s) 620
BmsI GCATC 2 cut(s) 145, 828
Bpu1102I GCTNAGC 2 cut(s) 315, 837
BpuEI CTTGAG 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 168, 482, 619
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 3 cut(s) 25, 26, 582
Bse3DI GCAATG 4 cut(s) 45, 315, 405, 470
BseBI CCWGG 1 cut(s) 620
BseDI CCNNGG 3 cut(s) 168, 482, 619
BseLI CCNNNNNNNGG 3 cut(s) 25, 26, 582
BseMI GCAATG 4 cut(s) 45, 315, 405, 470
BseMII CTCAG 4 cut(s) 306, 600, 836, 851
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 858
BseXI GCAGC 1 cut(s) 306
BseYI CCCAGC 1 cut(s) 190
BshFI GGCC 1 cut(s) 560
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 788
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 3 cut(s) 25, 26, 582
BsmAI GTCTC 2 cut(s) 564, 785
BsmFI GGGAC 1 cut(s) 2
BsnI GGCC 1 cut(s) 560
Bso31I GGTCTC 1 cut(s) 564
BsoBI CYCGRG 1 cut(s) 788
Bsp1286I GDGCHC 2 cut(s) 538, 858
Bsp143I GATC 3 cut(s) 58, 151, 259
Bsp1720I GCTNAGC 2 cut(s) 315, 837
BspACI CCGC 1 cut(s) 513
BspANI GGCC 1 cut(s) 560
BspCNI CTCAG 4 cut(s) 307, 601, 837, 850
BspLI GGNNCC 1 cut(s) 617
BspPI GGATC 2 cut(s) 159, 267
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 4 cut(s) 45, 315, 405, 470
BssECI CCNNGG 3 cut(s) 168, 482, 619
BssMI GATC 3 cut(s) 58, 151, 259
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 620
Bst4CI ACNGT 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 4 cut(s) 315, 609, 837, 845
BstKTI GATC 3 cut(s) 61, 154, 262
BstMAI GTCTC 2 cut(s) 564, 785
BstMBI GATC 3 cut(s) 58, 151, 259
BstNI CCWGG 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 618
BstSLI GKGCMC 1 cut(s) 858
BstV1I GCAGC 1 cut(s) 306
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 1 cut(s) 259
BsuRI GGCC 1 cut(s) 560
Cac8I GCNNGC 1 cut(s) 274
CaiI CAGNNNCTG 1 cut(s) 844
Cfr13I GGNCC 1 cut(s) 558
CseI GACGC 1 cut(s) 240
Csp6I GTAC 2 cut(s) 220, 785
CviAII CATG 4 cut(s) 155, 187, 199, 802
CviQI GTAC 2 cut(s) 220, 785
DdeI CTNAG 4 cut(s) 315, 609, 837, 845
DpnI GATC 3 cut(s) 60, 153, 261
DpnII GATC 3 cut(s) 58, 151, 259
Eco130I CCWWGG 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 564
Eco57I CTGAAG 2 cut(s) 228, 273
Eco88I CYCGRG 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 618
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 4 cut(s) 158, 190, 202, 805
FaqI GGGAC 1 cut(s) 2
FatI CATG 4 cut(s) 154, 186, 198, 801
FauI CCCGC 1 cut(s) 506
Fnu4HI GCNGC 1 cut(s) 320
Fsp4HI GCNGC 1 cut(s) 320
FspBI CTAG 2 cut(s) 147, 389
GluI GCNGC 1 cut(s) 320
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 1 cut(s) 560
HgaI GACGC 1 cut(s) 240
Hin1II CATG 4 cut(s) 158, 190, 202, 805
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 2 cut(s) 212, 398
HphI GGTGA 1 cut(s) 814
Hpy166II GTNNAC 2 cut(s) 94, 743
Hpy188I TCNGA 5 cut(s) 208, 230, 403, 486, 846
Hpy8I GTNNAC 2 cut(s) 94, 743
HpyAV CCTTC 2 cut(s) 79, 678
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 4 cut(s) 4, 45, 410, 727
HpyF3I CTNAG 4 cut(s) 315, 609, 837, 845
Hsp92II CATG 4 cut(s) 158, 190, 202, 805
Kzo9I GATC 3 cut(s) 58, 151, 259
LmnI GCTCC 3 cut(s) 11, 55, 133
LpnPI CCDG 7 cut(s) 82, 176, 258, 345, 605, 621, 632
Lsp1109I GCAGC 1 cut(s) 306
LweI GCATC 2 cut(s) 145, 828
MaeI CTAG 2 cut(s) 147, 389
MaeIII GTNAC 2 cut(s) 453, 643
MalI GATC 3 cut(s) 60, 153, 261
MboI GATC 3 cut(s) 58, 151, 259
MboII GAAGA 5 cut(s) 131, 133, 221, 785, 818
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 2 cut(s) 538, 858
MluCI AATT 4 cut(s) 5, 507, 636, 747
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 5 cut(s) 124, 388, 492, 606, 852
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 2 cut(s) 545, 750
MspR9I CCNGG 1 cut(s) 620
MvaI CCWGG 1 cut(s) 620
NdeII GATC 3 cut(s) 58, 151, 259
NlaIII CATG 4 cut(s) 158, 190, 202, 805
NlaIV GGNNCC 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 212, 398
PflMI CCANNNNNTGG 1 cut(s) 582
PkrI GCNGC 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 618
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 618
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 844
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 2 cut(s) 221, 786
RsaNI GTAC 2 cut(s) 220, 785
SaqAI TTAA 2 cut(s) 545, 750
SatI GCNGC 1 cut(s) 320
Sau3AI GATC 3 cut(s) 58, 151, 259
Sau96I GGNCC 1 cut(s) 558
ScrFI CCNGG 1 cut(s) 620
SduI GDGCHC 2 cut(s) 538, 858
SetI ASST 7 cut(s) 16, 321, 617, 666, 771, 827, 853
SfaNI GCATC 2 cut(s) 145, 828
SmlI CTYRAG 1 cut(s) 537
SmoI CTYRAG 1 cut(s) 537
SpeI ACTAGT 1 cut(s) 388
Sse9I AATT 4 cut(s) 5, 507, 636, 747
SsiI CCGC 1 cut(s) 513
SspMI CTAG 2 cut(s) 147, 389
StyD4I CCNGG 1 cut(s) 618
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 57
TaqII GACCGA 1 cut(s) 353
TasI AATT 4 cut(s) 5, 507, 636, 747
TfiI GAWTC 2 cut(s) 212, 398
Tru1I TTAA 2 cut(s) 545, 750
Tru9I TTAA 2 cut(s) 545, 750
TseI GCWGC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 98, 187, 812
Van91I CCANNNNNTGG 1 cut(s) 582
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 147, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.