Rorug01G0214900

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012482
Physical Location & Seq
Forward (+)
30546218 .. 30546697
480 bp
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UTR
Exon/CDS
Intron
Rorug01G0214900.1

Sequence Viewer

Length: 480 bp
ATGTTAATACTAACAAAGTTACCTCACAAAGATCTGCATCAACTGGCCAGAAAGTACGGTGACATCATGTACATGCGGTTAGGCCTTGTGCCAGCCATTATCGTCTCTTCCCCACGAGCAGCCGAGCTGTTCCTCAAAACCCACGACCTCGTGTTTGCGAGCAGGCCGCCTCACGAAGGTGCAAAGCATATTTCTTATGGCCAAAGGAGCTTGAGTTTTGCTGAGTATGGCTCTTACTGGCGTGACATGCGCAAGATGTGCACTCTTGAGTTACTTAGCAACCACAAAATCAACTCCTTCAAGTCAATGAGGAAAGAAGAGGTCGCCCTCATGGTGGATTCTATTCGAGAGTCTGCAGCCAAGGAACAACGTGTTTCGGTTGATCTGAGCTCCAAGGTGTCGTCTCTTAGCGCAGATATGACTTGCAGGATGGTGTTTGGGAAGAAATACATGGATGAGGAATTTAGTGAGGGGTTTTAA

Protein Analysis

159

Amino Acids

18.23

Weight (kDa)

9.23

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 6 - 155 3.5e-21 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012482)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45480 AT2G45480 AT2G45480
fragaria_vesca FvH4_7g12130 FvH4_7g12130
malus_domestica MD02G1201000.v1.1
prunus_persica Prupe.2G148100_v2.0.a1
pyrus_communis pycom02g16430
rosa_chinensis RchiOBHm_Chr1g0350881
rosa_laevigata RLG00000028446
rosa_multiflora Rmu_sc0014584.1_g000012
rosa_roxburghii Rroxscaffold_4G00304440
rosa_rugosa Rorug01G0214900 Rorug01G0215000
rosa_samantha Rh1AG229300 Rh1BG199300 Rh1CG214100 Rh1DG227200
rosa_wichuraiana Rw1G019870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 251
AciI CCGC 2 cut(s) 76, 167
AcoI YGGCCR 2 cut(s) 45, 199
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 2 cut(s) 56, 71
AfiI CCNNNNNNNGG 2 cut(s) 176, 334
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 1 cut(s) 301
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 3 cut(s) 127, 210, 390
AluI AGCT 3 cut(s) 127, 210, 390
Alw21I GWGCWC 2 cut(s) 263, 392
Alw26I GTCTC 2 cut(s) 109, 408
Alw44I GTGCAC 1 cut(s) 259
AoxI GGCC 4 cut(s) 45, 82, 164, 199
ApaLI GTGCAC 1 cut(s) 259
ApeKI GCWGC 2 cut(s) 119, 356
ApoI RAATTY 1 cut(s) 461
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
AspLEI GCGC 2 cut(s) 252, 413
AsuHPI GGTGA 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 263
BalI TGGCCA 2 cut(s) 47, 201
BanII GRGCYC 1 cut(s) 392
BauI CACGAG 2 cut(s) 114, 149
Bbv12I GWGCWC 2 cut(s) 263, 392
BbvI GCAGC 2 cut(s) 131, 368
BccI CCATC 1 cut(s) 424
BcoDI GTCTC 2 cut(s) 109, 408
BfmI CTRYAG 1 cut(s) 354
BglII AGATCT 1 cut(s) 31
BisI GCNGC 3 cut(s) 120, 167, 357
BlsI GCNGC 3 cut(s) 121, 168, 358
BmsI GCATC 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 215, 247
BpuEI CTTGAG 2 cut(s) 232, 287
BsaBI GATNNNNATC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 360, 393
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 334
Bse1I ACTGG 2 cut(s) 48, 242
Bse8I GATNNNNATC 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 360, 393
BseGI GGATG 2 cut(s) 435, 460
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 176, 334
BseMII CTCAG 2 cut(s) 213, 377
BseNI ACTGG 2 cut(s) 48, 242
BseSI GKGCMC 1 cut(s) 263
BseXI GCAGC 2 cut(s) 131, 368
BshFI GGCC 4 cut(s) 47, 84, 166, 201
BsiHKAI GWGCWC 2 cut(s) 263, 392
BslI CCNNNNNNNGG 2 cut(s) 176, 334
BsmAI GTCTC 2 cut(s) 109, 408
BsmBI CGTCTC 2 cut(s) 109, 408
BsnI GGCC 4 cut(s) 47, 84, 166, 201
Bsp1286I GDGCHC 2 cut(s) 263, 392
Bsp1407I TGTACA 1 cut(s) 69
Bsp143I GATC 2 cut(s) 31, 382
BspACI CCGC 2 cut(s) 76, 167
BspANI GGCC 4 cut(s) 47, 84, 166, 201
BspCNI CTCAG 2 cut(s) 214, 378
BspMAI CTGCAG 1 cut(s) 358
BsrGI TGTACA 1 cut(s) 69
BsrI ACTGG 2 cut(s) 48, 242
BssECI CCNNGG 2 cut(s) 360, 393
BssMI GATC 2 cut(s) 31, 382
BssSI CACGAG 2 cut(s) 114, 149
BssT1I CCWWGG 2 cut(s) 360, 393
Bst2BI CACGAG 2 cut(s) 114, 149
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 112, 312
BstAPI GCANNNNNTGC 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 69
BstC8I GCNNGC 3 cut(s) 93, 160, 164
BstDEI CTNAG 4 cut(s) 222, 275, 386, 407
BstENI CCTNNNNNAGG 1 cut(s) 174
BstF5I GGATG 2 cut(s) 435, 460
BstHHI GCGC 2 cut(s) 252, 413
BstKTI GATC 2 cut(s) 34, 385
BstMAI GTCTC 2 cut(s) 109, 408
BstMBI GATC 2 cut(s) 31, 382
BstMWI GCNNNNNNNGC 3 cut(s) 207, 247, 258
BstNSI RCATGY 2 cut(s) 76, 250
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 131, 368
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 4 cut(s) 47, 84, 166, 201
BtsCI GGATG 2 cut(s) 435, 460
Cac8I GCNNGC 3 cut(s) 93, 160, 164
CfoI GCGC 2 cut(s) 252, 413
Csp6I GTAC 2 cut(s) 55, 70
CviAII CATG 5 cut(s) 67, 73, 247, 331, 451
CviQI GTAC 2 cut(s) 55, 70
DdeI CTNAG 4 cut(s) 222, 275, 386, 407
DpnI GATC 2 cut(s) 33, 384
DpnII GATC 2 cut(s) 31, 382
EaeI YGGCCR 2 cut(s) 45, 199
Eam1104I CTCTTC 2 cut(s) 112, 312
EarI CTCTTC 2 cut(s) 112, 312
Ecl136II GAGCTC 1 cut(s) 390
Eco130I CCWWGG 2 cut(s) 360, 393
Eco147I AGGCCT 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 390
EcoNI CCTNNNNNAGG 1 cut(s) 174
EcoT14I CCWWGG 2 cut(s) 360, 393
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 2 cut(s) 360, 393
Esp3I CGTCTC 2 cut(s) 109, 408
FaeI CATG 5 cut(s) 70, 76, 250, 334, 454
FaiI YATR 9 cut(s) 68, 74, 189, 198, 228, 248, 332, 419, 452
FatI CATG 5 cut(s) 66, 72, 246, 330, 450
Fnu4HI GCNGC 3 cut(s) 120, 167, 357
FokI GGATG 2 cut(s) 442, 467
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 3 cut(s) 120, 167, 357
FspI TGCGCA 1 cut(s) 251
GlaI GCGC 2 cut(s) 251, 412
GluI GCNGC 3 cut(s) 120, 167, 357
HaeIII GGCC 4 cut(s) 47, 84, 166, 201
HhaI GCGC 2 cut(s) 252, 413
Hin1II CATG 5 cut(s) 70, 76, 250, 334, 454
Hin6I GCGC 2 cut(s) 250, 411
HinP1I GCGC 2 cut(s) 250, 411
HinfI GANTC 2 cut(s) 338, 350
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 261
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 3 cut(s) 173, 266, 347
Hpy8I GTNNAC 1 cut(s) 261
HpyAV CCTTC 2 cut(s) 170, 307
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 5 cut(s) 37, 182, 261, 356, 426
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 247, 258
HpyF3I CTNAG 4 cut(s) 222, 275, 386, 407
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 5 cut(s) 70, 76, 250, 334, 454
HspAI GCGC 2 cut(s) 250, 411
Kzo9I GATC 2 cut(s) 31, 382
LmnI GCTCC 2 cut(s) 207, 395
LpnPI CCDG 6 cut(s) 29, 61, 105, 148, 223, 412
Lsp1109I GCAGC 2 cut(s) 131, 368
LweI GCATC 1 cut(s) 46
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 4 cut(s) 18, 59, 242, 270
MalI GATC 2 cut(s) 33, 384
MboI GATC 2 cut(s) 31, 382
MboII GAAGA 3 cut(s) 99, 329, 454
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 2 cut(s) 263, 392
MlsI TGGCCA 2 cut(s) 47, 201
MluCI AATT 1 cut(s) 461
MluNI TGGCCA 2 cut(s) 47, 201
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 9 cut(s) 33, 143, 158, 180, 303, 313, 338, 451, 463
Mox20I TGGCCA 2 cut(s) 47, 201
MscI TGGCCA 2 cut(s) 47, 201
MseI TTAA 2 cut(s) 5, 478
MslI CAYNNNNRTG 2 cut(s) 71, 177
Msp20I TGGCCA 2 cut(s) 47, 201
MwoI GCNNNNNNNGC 3 cut(s) 207, 247, 258
NdeII GATC 2 cut(s) 31, 382
NlaIII CATG 5 cut(s) 70, 76, 250, 334, 454
NmeAIII GCCGAG 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 59, 242
NsbI TGCGCA 1 cut(s) 251
NspI RCATGY 2 cut(s) 76, 250
OliI CACNNNNGTG 1 cut(s) 177
PceI AGGCCT 1 cut(s) 84
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 3 cut(s) 121, 168, 358
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 358
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 2 cut(s) 56, 71
RsaNI GTAC 2 cut(s) 55, 70
RseI CAYNNNNRTG 2 cut(s) 71, 177
SacI GAGCTC 1 cut(s) 392
SaqAI TTAA 2 cut(s) 5, 478
SatI GCNGC 3 cut(s) 120, 167, 357
Sau3AI GATC 2 cut(s) 31, 382
SchI GAGTC 1 cut(s) 359
SduI GDGCHC 2 cut(s) 263, 392
SetI ASST 9 cut(s) 25, 129, 150, 181, 212, 324, 373, 392, 399
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 71, 177
SmlI CTYRAG 2 cut(s) 211, 266
SmoI CTYRAG 2 cut(s) 211, 266
Sse9I AATT 1 cut(s) 461
SseBI AGGCCT 1 cut(s) 84
SsiI CCGC 2 cut(s) 76, 167
SstI GAGCTC 1 cut(s) 392
StuI AGGCCT 1 cut(s) 84
StyI CCWWGG 2 cut(s) 360, 393
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 1 cut(s) 373
TaqI TCGA 1 cut(s) 346
TasI AATT 1 cut(s) 461
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 169
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 2 cut(s) 5, 478
Tru9I TTAA 2 cut(s) 5, 478
TseFI GTSAC 2 cut(s) 59, 242
TseI GCWGC 2 cut(s) 119, 356
Tsp45I GTSAC 2 cut(s) 59, 242
VneI GTGCAC 1 cut(s) 259
XagI CCTNNNNNAGG 1 cut(s) 174
XapI RAATTY 1 cut(s) 461
XceI RCATGY 2 cut(s) 76, 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.