Prupe.2G287700_v2.0.a1

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0011173
Physical Location & Seq
Forward (+)
28324854 .. 28329981
5128 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G287700.1

Sequence Viewer

Length: 726 bp
ATGGCTGAAACACGAACAAACAAGAGGAAAATGGGAGACAGAAGAGAAGCTGATTCCAGCAAAACAGCGTCAAAGTGGCCAACCATCAAAACAAAACCAAATCTTCAGATAACTTGTCTTAAAGATACTTATCTCTTTACTGTGCAGAATTTCCTCACATCTGCAGAATCAAAGGCTTTCGTTAAAGCTGTAGAATCTGTTGGGTTTGTTCACCAGGGGAGTCTTGGCCCAACAAAAGGCGAAGCTTATAGAGACAATGATAGAATCTCTGTGAATGATCCTGTTCTTGCAGACACAATTTGGGAGTCTGGTCTTGACAAATTATTTTCTGATATCAAAATTCGAGGGAAGGCTGCTGTTGGCTTAAACCCAAATATCAGATTCTATAGGTACAAGGTTGGGCAGCGCTTTGGAAGGCATATTGATGAAAGTAATGATCTTGGAGACGGAAAACGCACCCATTATACTTTGTTAATATACTTAAGTGGCGGTTTCAAACCAAAAGCTTCAAATGATAAGAGAAGTCCTAAGGATTCTTCCTCAGAGCCTCTGGTTGGAGGAGAAACTGTCTTCTACGGTTCGAGGAATACTGTTGTGGCAGAGGTGCCTCCCACTGAAGGAATGGCTCTTCTACACATTCATGGAGACAAATGTTTGTTGCATGAAGCCCGAAACGTTACTAAGGGTATCAAGTACGTTTTTCGTTCCGATGTAGTATTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

242

Amino Acids

26.83

Weight (kDa)

9.52

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51880
fragaria_vesca FvH4_7g28750 FvH4_7g28750
malus_domestica MD01G1193900.v1.1
prunus_persica Prupe.2G287700_v2.0.a1
pyrus_communis pycom01g20420
rosa_chinensis RchiOBHm_Chr1g0376301
rosa_laevigata RLG00000026613
rosa_multiflora Rmu_ssc0000116.1_g000061
rosa_roxburghii Rroxscaffold_4G00282220
rosa_rugosa Rorug01G0395100 Rorug01G0395200 Rorug01G0395300 Rorug01G0395300 Rorug01G0395400
rosa_samantha Rh1AG406500 Rh1BG370500 Rh1CG383300 Rh1DG400300
rosa_wichuraiana Rw1G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 604
AciI CCGC 1 cut(s) 489
AclI AACGTT 1 cut(s) 675
AclWI GGATC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 77
AcsI RAATTY 2 cut(s) 148, 339
AcuI CTGAAG 2 cut(s) 89, 636
AfaI GTAC 2 cut(s) 392, 695
AfeI AGCGCT 1 cut(s) 407
AfiI CCNNNNNNNGG 3 cut(s) 236, 554, 617
AflII CTTAAG 1 cut(s) 481
AgsI TTSAA 2 cut(s) 496, 510
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 4 cut(s) 50, 188, 245, 506
AluI AGCT 4 cut(s) 50, 188, 245, 506
Alw26I GTCTC 4 cut(s) 30, 246, 438, 639
AlwI GGATC 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 407
AoxI GGCC 2 cut(s) 77, 226
ApeKI GCWGC 2 cut(s) 353, 403
ApoI RAATTY 2 cut(s) 148, 339
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 528
BalI TGGCCA 1 cut(s) 79
BanI GGYRCC 1 cut(s) 604
BbsI GAAGAC 1 cut(s) 562
BbvI GCAGC 2 cut(s) 340, 415
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 215
BcoDI GTCTC 4 cut(s) 30, 246, 438, 639
BfmI CTRYAG 3 cut(s) 162, 189, 385
BfoI RGCGCY 1 cut(s) 409
BfrI CTTAAG 1 cut(s) 481
BisI GCNGC 2 cut(s) 354, 404
BlsI GCNGC 2 cut(s) 355, 405
Bme1390I CCNGG 1 cut(s) 215
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 606
BmrFI CCNGG 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 552, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 554, 617
Bse21I CCTNAGG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 3 cut(s) 236, 554, 617
BseMII CTCAG 1 cut(s) 555
BseRI GAGGAG 1 cut(s) 573
BseXI GCAGC 2 cut(s) 340, 415
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 2 cut(s) 79, 228
BshNI GGYRCC 1 cut(s) 604
BslI CCNNNNNNNGG 3 cut(s) 236, 554, 617
BsmAI GTCTC 4 cut(s) 30, 246, 438, 639
BsmBI CGTCTC 1 cut(s) 438
BsnI GGCC 2 cut(s) 79, 228
Bsp143I GATC 2 cut(s) 277, 436
BspACI CCGC 1 cut(s) 489
BspANI GGCC 2 cut(s) 79, 228
BspCNI CTCAG 1 cut(s) 554
BspLI GGNNCC 1 cut(s) 606
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 1 cut(s) 272
BspQI GCTCTTC 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 604
BspTI CTTAAG 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 277, 436
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 4 cut(s) 142, 568, 578, 592
Bst6I CTCTTC 2 cut(s) 37, 633
BstAFI CTTAAG 1 cut(s) 481
BstDEI CTNAG 3 cut(s) 528, 541, 681
BstH2I RGCGCY 1 cut(s) 409
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 2 cut(s) 280, 439
BstMAI GTCTC 4 cut(s) 30, 246, 438, 639
BstMBI GATC 2 cut(s) 277, 436
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 213
BstSFI CTRYAG 3 cut(s) 162, 189, 385
BstV1I GCAGC 2 cut(s) 340, 415
BstV2I GAAGAC 1 cut(s) 562
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 2 cut(s) 79, 228
BtsIMutI CAGTG 1 cut(s) 612
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 1 cut(s) 57
Csp6I GTAC 2 cut(s) 391, 694
CviAII CATG 2 cut(s) 641, 662
CviQI GTAC 2 cut(s) 391, 694
DdeI CTNAG 3 cut(s) 528, 541, 681
DpnI GATC 2 cut(s) 279, 438
DpnII GATC 2 cut(s) 277, 436
EaeI YGGCCR 1 cut(s) 77
Eam1104I CTCTTC 2 cut(s) 37, 633
EarI CTCTTC 2 cut(s) 37, 633
Eco32I GATATC 1 cut(s) 334
Eco47III AGCGCT 1 cut(s) 407
Eco57I CTGAAG 2 cut(s) 89, 636
Eco81I CCTNAGG 1 cut(s) 528
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 334
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 2 cut(s) 644, 665
FaiI YATR 7 cut(s) 249, 387, 420, 465, 478, 642, 663
FatI CATG 2 cut(s) 640, 661
Fnu4HI GCNGC 2 cut(s) 354, 404
Fsp4HI GCNGC 2 cut(s) 354, 404
GlaI GCGC 1 cut(s) 407
GluI GCNGC 2 cut(s) 354, 404
HaeII RGCGCY 1 cut(s) 409
HaeIII GGCC 2 cut(s) 79, 228
HgaI GACGC 1 cut(s) 57
HhaI GCGC 1 cut(s) 408
Hin1II CATG 2 cut(s) 644, 665
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HindIII AAGCTT 2 cut(s) 243, 504
HinfI GANTC 8 cut(s) 53, 167, 194, 220, 264, 305, 381, 533
HphI GGTGA 1 cut(s) 203
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 5 cut(s) 108, 331, 380, 544, 709
Hpy188III TCNNGA 1 cut(s) 314
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 3 cut(s) 343, 408, 611
HpyCH4III ACNGT 4 cut(s) 142, 568, 578, 592
HpyCH4IV ACGT 2 cut(s) 675, 696
HpyCH4V TGCA 4 cut(s) 145, 164, 290, 661
HpyF3I CTNAG 3 cut(s) 528, 541, 681
HpySE526I ACGT 2 cut(s) 675, 696
Hsp92II CATG 2 cut(s) 644, 665
HspAI GCGC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 277, 436
LguI GCTCTTC 1 cut(s) 633
LpnPI CCDG 6 cut(s) 70, 200, 227, 294, 294, 536
Lsp1109I GCAGC 2 cut(s) 340, 415
MaeII ACGT 2 cut(s) 675, 696
MaeIII GTNAC 1 cut(s) 676
MalI GATC 2 cut(s) 279, 438
MboI GATC 2 cut(s) 277, 436
MboII GAAGA 5 cut(s) 54, 95, 528, 562, 620
MlsI TGGCCA 1 cut(s) 79
MluCI AATT 4 cut(s) 148, 297, 320, 339
MluNI TGGCCA 1 cut(s) 79
MlyI GAGTC 2 cut(s) 229, 314
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 9 cut(s) 18, 164, 338, 550, 551, 558, 576, 595, 618
Mox20I TGGCCA 1 cut(s) 79
MscI TGGCCA 1 cut(s) 79
MseI TTAA 5 cut(s) 120, 183, 365, 473, 482
MslI CAYNNNNRTG 2 cut(s) 423, 639
Msp20I TGGCCA 1 cut(s) 79
MspCI CTTAAG 1 cut(s) 481
MspR9I CCNGG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 215
NdeII GATC 2 cut(s) 277, 436
NlaIII CATG 2 cut(s) 644, 665
NlaIV GGNNCC 1 cut(s) 606
PciSI GCTCTTC 1 cut(s) 633
PfeI GAWTC 6 cut(s) 53, 167, 194, 264, 381, 533
PkrI GCNGC 2 cut(s) 355, 405
PleI GAGTC 2 cut(s) 228, 313
PpsI GAGTC 2 cut(s) 228, 313
Psp1406I AACGTT 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 606
PspPI GGNCC 1 cut(s) 227
PstI CTGCAG 1 cut(s) 166
RsaI GTAC 2 cut(s) 392, 695
RsaNI GTAC 2 cut(s) 391, 694
RseI CAYNNNNRTG 2 cut(s) 423, 639
SapI GCTCTTC 1 cut(s) 633
SaqAI TTAA 5 cut(s) 120, 183, 365, 473, 482
SatI GCNGC 2 cut(s) 354, 404
Sau3AI GATC 2 cut(s) 277, 436
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 2 cut(s) 229, 314
ScrFI CCNGG 1 cut(s) 215
SetI ASST 9 cut(s) 52, 190, 247, 392, 399, 508, 606, 678, 699
SfcI CTRYAG 3 cut(s) 162, 189, 385
SmiMI CAYNNNNRTG 2 cut(s) 423, 639
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 4 cut(s) 148, 297, 320, 339
SsiI CCGC 1 cut(s) 489
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 4 cut(s) 142, 568, 578, 592
TaiI ACGT 2 cut(s) 678, 699
TaqI TCGA 2 cut(s) 343, 581
TasI AATT 4 cut(s) 148, 297, 320, 339
TfiI GAWTC 6 cut(s) 53, 167, 194, 264, 381, 533
Tru1I TTAA 5 cut(s) 120, 183, 365, 473, 482
Tru9I TTAA 5 cut(s) 120, 183, 365, 473, 482
TscAI CASTG 1 cut(s) 619
TseI GCWGC 2 cut(s) 353, 403
TspDTI ATGAA 3 cut(s) 441, 629, 678
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 1 cut(s) 619
Vha464I CTTAAG 1 cut(s) 481
XapI RAATTY 2 cut(s) 148, 339
XcmI CCANNNNNNNNNTGG 2 cut(s) 221, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.