Rorug01G0395100

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011173
Physical Location & Seq
Forward (+)
50507893 .. 50509596
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0395100.1

Sequence Viewer

Length: 933 bp
ATGCATGCCAACAACCCCTCCATTCATTCATCAAAAACGGTTAAAAAACTCATCCTCTCTCTTCAACTCCATCCCCCATTTCTTCTTCTTCTTCTTCGAAACTCACAAAGGGCACCATCTAAATCTTCTTCTTCGTCGAAACTCACAGACGCCATGAGCGGCGGTGGTGATAATAGAGTGAAGGGGTCGTGGAGTCCTCAGGAGGACGCAACGCTGATCAAGCTGGTGGCGGAGCACGGCCCGCGCAACTGGTCCTTGATCAGCTCCGGCATCCCCGGCCGGTCCGGCAAGTCGTGTAGGCTGAGGTGGTGCAACCAGCTCAGCCCCGACGTGCAGCACAAGCCTTTCTCGCCGGCCGAGGACGCCGCGATCGTCCGGGCACACGCGCTCCACGGCAACAAGTGGGCCACCATCGCCCGGCTCCTCCCCGGCCGCACCGACAATGCCATCAAGAACCACTGGAACTCCACGCTCCGGCGGCGGAGGCTGGCCGAGTCGAGCTCGGCCTCTTCCGAGTCCAACTCGGCCGCGTTGAAGCGGTTGATTGGCGCCACGTCGGGCGACCCGGAGATGAAGCGGCAGTGCTTGCGTGGGGCGTCGGCGGAGGAGGATAGTTTCAAAGCGGTTGCGGCGGGAGTGGAGGGCGTGCTGACGTCACTGACGCTGTCGCCGCCTGGGGAGAAAGTGGAAAACGAGGTCGTTTTGAAGAGTGAGGAGGTGGGGGAGGAGGACGAAGAAGACAATGATGATCATCAAGACGAGGCGGTGGATGACGAGGAGAAATGTACGGTGGAGATGGAGGAGACGTGTCTGATGACGATGATGCAGCGGATGATAGCCCAGGAGGTGAGGAACTACATCGAGAGCTTACGGGCCGAGAATGGGCTAGCATTTGGGCTACAGTCGACGGTCCAAAATGATCCAAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

310

Amino Acids

33.93

Weight (kDa)

6.03

Isoelectric Point (pI)

63.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 61 - 107 3e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 64 - 122 3.6e-16 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 114 - 157 7.3e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 116 - 161 9.6e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51880
fragaria_vesca FvH4_7g28750 FvH4_7g28750
malus_domestica MD01G1193900.v1.1
prunus_persica Prupe.2G287700_v2.0.a1
pyrus_communis pycom01g20420
rosa_chinensis RchiOBHm_Chr1g0376301
rosa_laevigata RLG00000026613
rosa_multiflora Rmu_ssc0000116.1_g000061
rosa_roxburghii Rroxscaffold_4G00282220
rosa_rugosa Rorug01G0395100 Rorug01G0395200 Rorug01G0395300 Rorug01G0395300 Rorug01G0395400
rosa_samantha Rh1AG406500 Rh1BG370500 Rh1CG383300 Rh1DG400300
rosa_wichuraiana Rw1G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 656
AccB1I GGYRCC 2 cut(s) 112, 548
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 905
AccII CGCG 4 cut(s) 244, 368, 386, 530
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 5 cut(s) 277, 354, 430, 489, 525
AcyI GRCGYC 5 cut(s) 150, 363, 549, 596, 653
AfaI GTAC 1 cut(s) 787
AfiI CCNNNNNNNGG 3 cut(s) 417, 474, 882
AflIII ACRYGT 1 cut(s) 806
AgsI TTSAA 4 cut(s) 65, 535, 619, 706
AjiI CACGTC 3 cut(s) 331, 555, 807
AjnI CCWGG 2 cut(s) 673, 840
AluBI AGCT 5 cut(s) 223, 264, 319, 501, 867
AluI AGCT 5 cut(s) 223, 264, 319, 501, 867
Alw21I GWGCWC 2 cut(s) 237, 503
Alw26I GTCTC 1 cut(s) 797
AlwI GGATC 1 cut(s) 914
AoxI GGCC 9 cut(s) 238, 277, 354, 405, 430, 489, 504, 525, 873
ApeKI GCWGC 2 cut(s) 334, 826
AspLEI GCGC 3 cut(s) 246, 388, 551
AspS9I GGNCC 6 cut(s) 239, 252, 282, 405, 873, 910
AsuC2I CCSGG 5 cut(s) 276, 377, 418, 429, 566
AsuHPI GGTGA 2 cut(s) 179, 859
AsuII TTCGAA 1 cut(s) 97
AsuNHI GCTAGC 1 cut(s) 886
AvaII GGWCC 3 cut(s) 252, 282, 910
AxyI CCTNAGG 1 cut(s) 198
BaeGI GKGCMC 2 cut(s) 115, 382
BanI GGYRCC 2 cut(s) 112, 548
BanII GRGCYC 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 2 cut(s) 237, 503
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 346, 838
BccI CCATC 5 cut(s) 78, 124, 419, 455, 790
BceAI ACGGC 2 cut(s) 253, 409
BciT130I CCWGG 2 cut(s) 675, 842
BclI TGATCA 3 cut(s) 216, 258, 748
BcnI CCSGG 5 cut(s) 276, 377, 418, 429, 566
BcoDI GTCTC 1 cut(s) 797
BfaI CTAG 1 cut(s) 887
BfmI CTRYAG 1 cut(s) 899
BfoI RGCGCY 1 cut(s) 552
BglI GCCNNNNNGGC 1 cut(s) 285
BlpI GCTNAGC 1 cut(s) 320
Bme1390I CCNGG 7 cut(s) 276, 377, 418, 429, 566, 675, 842
Bme18I GGWCC 3 cut(s) 252, 282, 910
BmgBI CACGTC 3 cut(s) 331, 555, 807
BmgT120I GGNCC 6 cut(s) 239, 252, 282, 405, 873, 910
BmiI GGNNCC 3 cut(s) 114, 422, 550
BmrFI CCNGG 7 cut(s) 276, 377, 418, 429, 566, 675, 842
BmsI GCATC 2 cut(s) 279, 813
BmtI GCTAGC 1 cut(s) 890
BpiI GAAGAC 1 cut(s) 744
BplI GAGNNNNNCTC 4 cut(s) 485, 517, 506, 538
Bpu10I CCTNAGC 1 cut(s) 302
Bpu1102I GCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 97
BpuMI CCSGG 5 cut(s) 276, 377, 418, 429, 566
BsaBI GATNNNNATC 1 cut(s) 750
BsaHI GRCGYC 5 cut(s) 150, 363, 549, 596, 653
BsaJI CCNNGG 6 cut(s) 274, 357, 391, 427, 674, 840
BsaXI ACNNNNNCTCC 7 cut(s) 32, 372, 402, 449, 479, 791, 821
Bsc4I CCNNNNNNNGG 3 cut(s) 417, 474, 882
Bse118I RCCGGY 2 cut(s) 279, 352
Bse1I ACTGG 2 cut(s) 254, 464
Bse21I CCTNAGG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 2 cut(s) 675, 842
BseDI CCNNGG 6 cut(s) 274, 357, 391, 427, 674, 840
BseGI GGATG 5 cut(s) 51, 70, 270, 775, 837
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 3 cut(s) 417, 474, 882
BseMII CTCAG 3 cut(s) 212, 293, 334
BseNI ACTGG 2 cut(s) 254, 464
BseRI GAGGAG 6 cut(s) 413, 620, 728, 740, 791, 815
BseSI GKGCMC 2 cut(s) 115, 382
BseX3I CGGCCG 4 cut(s) 277, 354, 430, 525
BseXI GCAGC 2 cut(s) 346, 838
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 4 cut(s) 244, 368, 386, 530
Bsh1285I CGRYCG 5 cut(s) 280, 357, 372, 433, 528
BshFI GGCC 9 cut(s) 240, 279, 356, 407, 432, 491, 506, 527, 875
BshNI GGYRCC 2 cut(s) 112, 548
BsiEI CGRYCG 5 cut(s) 280, 357, 372, 433, 528
BsiHKAI GWGCWC 2 cut(s) 237, 503
BslI CCNNNNNNNGG 3 cut(s) 417, 474, 882
BsmAI GTCTC 1 cut(s) 797
BsmBI CGTCTC 1 cut(s) 797
BsnI GGCC 9 cut(s) 240, 279, 356, 407, 432, 491, 506, 527, 875
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 4 cut(s) 115, 237, 382, 503
Bsp143I GATC 5 cut(s) 216, 258, 369, 748, 919
Bsp1720I GCTNAGC 1 cut(s) 320
BspANI GGCC 9 cut(s) 240, 279, 356, 407, 432, 491, 506, 527, 875
BspCNI CTCAG 3 cut(s) 211, 294, 333
BspFNI CGCG 4 cut(s) 244, 368, 386, 530
BspLI GGNNCC 3 cut(s) 114, 422, 550
BspOI GCTAGC 1 cut(s) 890
BspPI GGATC 1 cut(s) 914
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 2 cut(s) 112, 548
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 279, 352
BsrI ACTGG 2 cut(s) 254, 464
BssAI RCCGGY 2 cut(s) 279, 352
BssECI CCNNGG 6 cut(s) 274, 357, 391, 427, 674, 840
BssMI GATC 5 cut(s) 216, 258, 369, 748, 919
BssNI GRCGYC 5 cut(s) 150, 363, 549, 596, 653
Bst2UI CCWGG 2 cut(s) 675, 842
Bst4CI ACNGT 4 cut(s) 40, 790, 903, 910
Bst6I CTCTTC 3 cut(s) 66, 514, 701
BstACI GRCGYC 5 cut(s) 150, 363, 549, 596, 653
BstAPI GCANNNNNTGC 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 7 cut(s) 6, 242, 354, 489, 587, 647, 888
BstDEI CTNAG 3 cut(s) 198, 302, 320
BstDSI CCRYGG 1 cut(s) 391
BstF5I GGATG 5 cut(s) 51, 70, 270, 775, 837
BstFNI CGCG 4 cut(s) 244, 368, 386, 530
BstH2I RGCGCY 1 cut(s) 552
BstHHI GCGC 3 cut(s) 246, 388, 551
BstKTI GATC 5 cut(s) 219, 261, 372, 751, 922
BstMAI GTCTC 1 cut(s) 797
BstMBI GATC 5 cut(s) 216, 258, 369, 748, 919
BstMCI CGRYCG 5 cut(s) 280, 357, 372, 433, 528
BstNI CCWGG 2 cut(s) 675, 842
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 7 cut(s) 274, 375, 416, 427, 564, 673, 840
BstSFI CTRYAG 1 cut(s) 899
BstSLI GKGCMC 2 cut(s) 115, 382
BstUI CGCG 4 cut(s) 244, 368, 386, 530
BstV1I GCAGC 2 cut(s) 346, 838
BstV2I GAAGAC 1 cut(s) 744
BstZI CGGCCG 4 cut(s) 277, 354, 430, 525
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 9 cut(s) 240, 279, 356, 407, 432, 491, 506, 527, 875
BtgI CCRYGG 1 cut(s) 391
BtgZI GCGATG 1 cut(s) 397
BtrI CACGTC 3 cut(s) 331, 555, 807
BtsCI GGATG 5 cut(s) 51, 70, 270, 775, 837
BtsI GCAGTG 1 cut(s) 587
BtsIMutI CAGTG 3 cut(s) 457, 587, 656
Cac8I GCNNGC 7 cut(s) 6, 242, 354, 489, 587, 647, 888
CfoI GCGC 3 cut(s) 246, 388, 551
Cfr10I RCCGGY 2 cut(s) 279, 352
Cfr13I GGNCC 6 cut(s) 239, 252, 282, 405, 873, 910
CpoI CGGWCCG 1 cut(s) 282
CseI GACGC 5 cut(s) 158, 215, 371, 585, 670
Csp6I GTAC 1 cut(s) 786
CspI CGGWCCG 1 cut(s) 282
CviAII CATG 2 cut(s) 5, 154
CviQI GTAC 1 cut(s) 786
DdeI CTNAG 3 cut(s) 198, 302, 320
DinI GGCGCC 1 cut(s) 550
DpnI GATC 5 cut(s) 218, 260, 371, 750, 921
DpnII GATC 5 cut(s) 216, 258, 369, 748, 919
EaeI YGGCCR 5 cut(s) 277, 354, 430, 489, 525
EagI CGGCCG 4 cut(s) 277, 354, 430, 525
Eam1104I CTCTTC 3 cut(s) 66, 514, 701
EarI CTCTTC 3 cut(s) 66, 514, 701
EciI GGCGGA 3 cut(s) 245, 496, 617
Ecl136II GAGCTC 1 cut(s) 501
EclXI CGGCCG 4 cut(s) 277, 354, 430, 525
Eco24I GRGCYC 1 cut(s) 503
Eco47I GGWCC 3 cut(s) 252, 282, 910
Eco52I CGGCCG 4 cut(s) 277, 354, 430, 525
Eco53kI GAGCTC 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 501
EcoRII CCWGG 2 cut(s) 673, 840
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 503
EgeI GGCGCC 1 cut(s) 550
EheI GGCGCC 1 cut(s) 550
Esp3I CGTCTC 1 cut(s) 797
FaeI CATG 2 cut(s) 8, 157
FaiI YATR 2 cut(s) 6, 155
FatI CATG 2 cut(s) 4, 153
FauI CCCGC 2 cut(s) 249, 625
FbaI TGATCA 3 cut(s) 216, 258, 748
FblI GTMKAC 1 cut(s) 905
FokI GGATG 5 cut(s) 38, 57, 257, 782, 844
FriOI GRGCYC 1 cut(s) 503
FspBI CTAG 1 cut(s) 887
GlaI GCGC 3 cut(s) 245, 387, 550
HaeII RGCGCY 1 cut(s) 552
HaeIII GGCC 9 cut(s) 240, 279, 356, 407, 432, 491, 506, 527, 875
HgaI GACGC 5 cut(s) 158, 215, 371, 585, 670
HhaI GCGC 3 cut(s) 246, 388, 551
Hin1I GRCGYC 5 cut(s) 150, 363, 549, 596, 653
Hin1II CATG 2 cut(s) 8, 157
Hin6I GCGC 3 cut(s) 244, 386, 549
HinP1I GCGC 3 cut(s) 244, 386, 549
HincII GTYRAC 1 cut(s) 906
HindII GTYRAC 1 cut(s) 906
HinfI GANTC 3 cut(s) 193, 494, 515
HphI GGTGA 2 cut(s) 179, 859
Hpy166II GTNNAC 1 cut(s) 906
Hpy188I TCNGA 2 cut(s) 514, 813
Hpy188III TCNNGA 4 cut(s) 200, 451, 755, 862
Hpy8I GTNNAC 1 cut(s) 906
Hpy99I CGWCG 5 cut(s) 139, 332, 559, 601, 910
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 4 cut(s) 40, 790, 903, 910
HpyCH4IV ACGT 4 cut(s) 330, 554, 653, 806
HpyCH4V TGCA 4 cut(s) 4, 312, 334, 826
HpyF3I CTNAG 3 cut(s) 198, 302, 320
HpySE526I ACGT 4 cut(s) 330, 554, 653, 806
Hsp92I GRCGYC 5 cut(s) 150, 363, 549, 596, 653
Hsp92II CATG 2 cut(s) 8, 157
HspAI GCGC 3 cut(s) 244, 386, 549
KasI GGCGCC 1 cut(s) 548
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
Ksp22I TGATCA 3 cut(s) 216, 258, 748
Kzo9I GATC 5 cut(s) 216, 258, 369, 748, 919
LmnI GCTCC 5 cut(s) 232, 269, 393, 426, 477
Lsp1109I GCAGC 2 cut(s) 346, 838
LweI GCATC 2 cut(s) 279, 813
MaeI CTAG 1 cut(s) 887
MaeII ACGT 4 cut(s) 330, 554, 653, 806
MaeIII GTNAC 1 cut(s) 654
MalI GATC 5 cut(s) 218, 260, 371, 750, 921
MbiI CCGCTC 1 cut(s) 159
MboI GATC 5 cut(s) 216, 258, 369, 748, 919
MhlI GDGCHC 4 cut(s) 115, 237, 382, 503
Mly113I GGCGCC 1 cut(s) 549
MlyI GAGTC 3 cut(s) 202, 503, 524
MmeI TCCRAC 1 cut(s) 543
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 352
MseI TTAA 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 829
MspR9I CCNGG 7 cut(s) 276, 377, 418, 429, 566, 675, 842
MvaI CCWGG 2 cut(s) 675, 842
MvnI CGCG 4 cut(s) 244, 368, 386, 530
NaeI GCCGGC 1 cut(s) 354
NarI GGCGCC 1 cut(s) 549
NciI CCSGG 5 cut(s) 276, 377, 418, 429, 566
NdeII GATC 5 cut(s) 216, 258, 369, 748, 919
NgoMIV GCCGGC 1 cut(s) 352
NheI GCTAGC 1 cut(s) 886
NlaIII CATG 2 cut(s) 8, 157
NlaIV GGNNCC 3 cut(s) 114, 422, 550
NmeAIII GCCGAG 5 cut(s) 382, 482, 503, 517, 901
NmuCI GTSAC 1 cut(s) 654
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
NspV TTCGAA 1 cut(s) 97
PaeI GCATGC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PdiI GCCGGC 1 cut(s) 354
PflFI GACNNNGTC 1 cut(s) 664
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 3 cut(s) 201, 502, 523
PluTI GGCGCC 1 cut(s) 552
PpsI GAGTC 3 cut(s) 201, 502, 523
Psp124BI GAGCTC 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 673, 840
PspGI CCWGG 2 cut(s) 673, 840
PspN4I GGNNCC 3 cut(s) 114, 422, 550
PspPI GGNCC 6 cut(s) 239, 252, 282, 405, 873, 910
PsyI GACNNNGTC 1 cut(s) 664
PvuI CGATCG 1 cut(s) 372
RsaI GTAC 1 cut(s) 787
RsaNI GTAC 1 cut(s) 786
Rsr2I CGGWCCG 1 cut(s) 282
RsrII CGGWCCG 1 cut(s) 282
SacI GAGCTC 1 cut(s) 503
SalI GTCGAC 1 cut(s) 904
SaqAI TTAA 1 cut(s) 42
Sau3AI GATC 5 cut(s) 216, 258, 369, 748, 919
Sau96I GGNCC 6 cut(s) 239, 252, 282, 405, 873, 910
SchI GAGTC 3 cut(s) 202, 503, 524
ScrFI CCNGG 7 cut(s) 276, 377, 418, 429, 566, 675, 842
SduI GDGCHC 4 cut(s) 115, 237, 382, 503
SfaNI GCATC 2 cut(s) 279, 813
SfcI CTRYAG 1 cut(s) 899
SfoI GGCGCC 1 cut(s) 550
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 3 cut(s) 252, 282, 910
SphI GCATGC 1 cut(s) 8
SspDI GGCGCC 1 cut(s) 548
SspMI CTAG 1 cut(s) 887
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 7 cut(s) 274, 375, 416, 427, 564, 673, 840
TaaI ACNGT 4 cut(s) 40, 790, 903, 910
TaiI ACGT 4 cut(s) 333, 557, 656, 809
TaqI TCGA 5 cut(s) 97, 137, 497, 861, 905
TauI GCSGC 8 cut(s) 162, 368, 435, 481, 530, 580, 632, 673
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 3 cut(s) 464, 587, 663
TseFI GTSAC 1 cut(s) 654
TseI GCWGC 2 cut(s) 334, 826
Tsp45I GTSAC 1 cut(s) 654
TspDTI ATGAA 3 cut(s) 14, 18, 587
TspRI CASTG 3 cut(s) 464, 587, 663
Tth111I GACNNNGTC 1 cut(s) 664
VpaK11BI GGWCC 3 cut(s) 252, 282, 910
XceI RCATGY 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 905
XspI CTAG 1 cut(s) 887
ZraI GACGTC 1 cut(s) 654
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.