Rw1G036080

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0011173
Physical Location & Seq
Forward (+)
63957751 .. 63959061
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G036080.1

Sequence Viewer

Length: 744 bp
ATGGCTGAAACAGAAACAAGGAAGAGGAAGATGGGAGAGAGAAGAGAAGCTGATTCCAATTCCAGGACAGCAACGCCAAAGTGGCCAACAATCAAACCAAAACAAAACATTCAGATAACCCGTCTCAAAGACAATGATCTTTTCACCGTACATAATTTCTTCACATCTGCAGAATCAAAGGCTTTTGTTAAGGTTGCAGAGTCTATTGGGTTTGTTCACCAAGGAAGTCTTGGCCCAACAAAAGGTGAAGCTTTTAGAGACAATGATAGAATCTCTGTGAACGATCCTGTTCTTGCTGATTCTATATGGAATTCTGGGCTCAGCAAATTGTTTTCTGACATCAAAATTCGAAGGGAGGTTGCTGTTGGCTTAAACCCAAATATCAGATTCTACAGGTACAAGGTTGGTCAGCGATTTGGGAGGCATATTGATGAAAGTGTTGATCTTGAAGATGGAAAACGCACGCATTATACTTTGTTGATATATTTGAGTGGCGGTTTCAAACCGAAAGCGAAAAATGATGTGAGCAGTCATCAGGATTCATCCTCAGAGCCTCTGGTTGGAGGAGGACCGTCTTTTATGGCTCAAGGAATAGTGTTGTGGCTGAGGTGTCTCCCGTTGAAGGGATGGCTCTTCTGCACATTCATGGGCACAAATGTATGCTGCATGAAGCTCGAAATGTTACTAAGGGTATCAAGTATGTGTTTCGTTCAGATGTTGTATTTGCATGATGTAAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

247

Amino Acids

27.92

Weight (kDa)

9.38

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51880
fragaria_vesca FvH4_7g28750 FvH4_7g28750
malus_domestica MD01G1193900.v1.1
prunus_persica Prupe.2G287700_v2.0.a1
pyrus_communis pycom01g20420
rosa_chinensis RchiOBHm_Chr1g0376301
rosa_laevigata RLG00000026613
rosa_multiflora Rmu_ssc0000116.1_g000061
rosa_roxburghii Rroxscaffold_4G00282220
rosa_rugosa Rorug01G0395100 Rorug01G0395200 Rorug01G0395300 Rorug01G0395300 Rorug01G0395400
rosa_samantha Rh1AG406500 Rh1BG370500 Rh1CG383300 Rh1DG400300
rosa_wichuraiana Rw1G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 495
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 2 cut(s) 310, 345
AfaI GTAC 2 cut(s) 150, 398
AfiI CCNNNNNNNGG 5 cut(s) 63, 242, 560, 622, 623
AgsI TTSAA 3 cut(s) 449, 502, 622
AjnI CCWGG 1 cut(s) 62
AluBI AGCT 3 cut(s) 50, 251, 673
AluI AGCT 3 cut(s) 50, 251, 673
Alw26I GTCTC 3 cut(s) 128, 252, 617
AlwI GGATC 1 cut(s) 278
AoxI GGCC 2 cut(s) 83, 232
ApeKI GCWGC 1 cut(s) 663
ApoI RAATTY 2 cut(s) 310, 345
AspS9I GGNCC 2 cut(s) 233, 569
AsuHPI GGTGA 3 cut(s) 136, 209, 257
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 653
BalI TGGCCA 1 cut(s) 85
BanII GRGCYC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 605
BbvI GCAGC 1 cut(s) 650
BccI CCATC 3 cut(s) 25, 446, 621
BcgI CGANNNNNNTGC 2 cut(s) 655, 689
BciT130I CCWGG 1 cut(s) 64
BcoDI GTCTC 3 cut(s) 128, 252, 617
BfmI CTRYAG 2 cut(s) 168, 391
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 1 cut(s) 664
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 665
Bme1390I CCNGG 1 cut(s) 64
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 233, 569
BmrFI CCNGG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 605
Bpu1102I GCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 570
BsaJI CCNNGG 1 cut(s) 220
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 242, 560, 622, 623
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 542, 632
BseLI CCNNNNNNNGG 5 cut(s) 63, 242, 560, 622, 623
BseMII CTCAG 3 cut(s) 334, 561, 596
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 653
BseXI GCAGC 1 cut(s) 650
BsgI GTGCAG 1 cut(s) 622
BshFI GGCC 2 cut(s) 85, 234
BslI CCNNNNNNNGG 5 cut(s) 63, 242, 560, 622, 623
BsmAI GTCTC 3 cut(s) 128, 252, 617
BsmBI CGTCTC 1 cut(s) 128
BsnI GGCC 2 cut(s) 85, 234
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 321, 653
Bsp143I GATC 3 cut(s) 136, 283, 442
Bsp1720I GCTNAGC 1 cut(s) 320
BspACI CCGC 1 cut(s) 495
BspANI GGCC 2 cut(s) 85, 234
BspCNI CTCAG 3 cut(s) 333, 560, 597
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 1 cut(s) 278
BspQI GCTCTTC 1 cut(s) 638
BspT104I TTCGAA 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 136, 283, 442
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 148, 573
Bst6I CTCTTC 3 cut(s) 17, 37, 638
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 4 cut(s) 320, 547, 605, 686
BstF5I GGATG 2 cut(s) 542, 632
BstKTI GATC 3 cut(s) 139, 286, 445
BstMAI GTCTC 3 cut(s) 128, 252, 617
BstMBI GATC 3 cut(s) 136, 283, 442
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstNI CCWGG 1 cut(s) 64
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 2 cut(s) 168, 391
BstSLI GKGCMC 1 cut(s) 653
BstV1I GCAGC 1 cut(s) 650
BsuRI GGCC 2 cut(s) 85, 234
BtsCI GGATG 2 cut(s) 542, 632
Cac8I GCNNGC 1 cut(s) 464
Cfr13I GGNCC 2 cut(s) 233, 569
Csp6I GTAC 2 cut(s) 149, 397
CviAII CATG 3 cut(s) 646, 667, 728
CviQI GTAC 2 cut(s) 149, 397
DdeI CTNAG 4 cut(s) 320, 547, 605, 686
DpnI GATC 3 cut(s) 138, 285, 444
DpnII GATC 3 cut(s) 136, 283, 442
EaeI YGGCCR 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 17, 37, 638
EarI CTCTTC 3 cut(s) 17, 37, 638
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 569
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 3 cut(s) 649, 670, 731
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 3 cut(s) 645, 666, 727
Fnu4HI GCNGC 1 cut(s) 664
FokI GGATG 2 cut(s) 529, 639
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 664
GluI GCNGC 1 cut(s) 664
HaeIII GGCC 2 cut(s) 85, 234
Hin1II CATG 3 cut(s) 649, 670, 731
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 7 cut(s) 53, 173, 200, 270, 299, 387, 539
HphI GGTGA 3 cut(s) 136, 209, 257
Hpy166II GTNNAC 2 cut(s) 217, 280
Hpy188I TCNGA 5 cut(s) 114, 337, 386, 550, 714
Hpy188III TCNNGA 2 cut(s) 446, 536
Hpy8I GTNNAC 2 cut(s) 217, 280
HpyAV CCTTC 2 cut(s) 345, 616
HpyCH4III ACNGT 2 cut(s) 148, 573
HpyCH4V TGCA 5 cut(s) 170, 197, 639, 666, 727
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpyF3I CTNAG 4 cut(s) 320, 547, 605, 686
Hsp92II CATG 3 cut(s) 649, 670, 731
Kzo9I GATC 3 cut(s) 136, 283, 442
LguI GCTCTTC 1 cut(s) 638
LpnPI CCDG 7 cut(s) 49, 76, 300, 300, 379, 521, 542
Lsp1109I GCAGC 1 cut(s) 650
MaeIII GTNAC 1 cut(s) 681
MalI GATC 3 cut(s) 138, 285, 444
MboI GATC 3 cut(s) 136, 283, 442
MboII GAAGA 6 cut(s) 34, 40, 54, 151, 461, 625
MhlI GDGCHC 2 cut(s) 321, 653
MlsI TGGCCA 1 cut(s) 85
MluCI AATT 5 cut(s) 58, 154, 310, 326, 345
MluNI TGGCCA 1 cut(s) 85
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 8 cut(s) 18, 349, 414, 556, 557, 560, 564, 600
Mox20I TGGCCA 1 cut(s) 85
MscI TGGCCA 1 cut(s) 85
MseI TTAA 2 cut(s) 189, 371
MslI CAYNNNNRTG 2 cut(s) 429, 644
Msp20I TGGCCA 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeII GATC 3 cut(s) 136, 283, 442
NlaIII CATG 3 cut(s) 649, 670, 731
NspV TTCGAA 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 638
PfeI GAWTC 6 cut(s) 53, 173, 270, 299, 387, 539
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 1 cut(s) 665
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspPI GGNCC 2 cut(s) 233, 569
PstI CTGCAG 1 cut(s) 172
RsaI GTAC 2 cut(s) 150, 398
RsaNI GTAC 2 cut(s) 149, 397
RseI CAYNNNNRTG 2 cut(s) 429, 644
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 2 cut(s) 189, 371
SatI GCNGC 1 cut(s) 664
Sau3AI GATC 3 cut(s) 136, 283, 442
Sau96I GGNCC 2 cut(s) 233, 569
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 321, 653
SetI ASST 9 cut(s) 52, 195, 247, 253, 360, 398, 405, 611, 675
SfcI CTRYAG 2 cut(s) 168, 391
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 2 cut(s) 429, 644
SmlI CTYRAG 1 cut(s) 585
SmoI CTYRAG 1 cut(s) 585
Sse9I AATT 5 cut(s) 58, 154, 310, 326, 345
SsiI CCGC 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 148, 573
TaqI TCGA 2 cut(s) 349, 675
TasI AATT 5 cut(s) 58, 154, 310, 326, 345
TfiI GAWTC 6 cut(s) 53, 173, 270, 299, 387, 539
Tru1I TTAA 2 cut(s) 189, 371
Tru9I TTAA 2 cut(s) 189, 371
TseI GCWGC 1 cut(s) 663
TspDTI ATGAA 4 cut(s) 447, 531, 634, 683
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 2 cut(s) 310, 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.