RchiOBHm_Chr1g0376301

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011173
Physical Location & Seq
Forward (+)
63550195 .. 63551508
1314 bp
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UTR
Exon/CDS
Intron
PRQ59995

Sequence Viewer

Length: 732 bp
ATGGCTGAAACAGAAACAAGGAAGAGGAAGATGGGAGAGAGAAGAGAAGCTGATTCCAATTCCAGGACAGCAACACCAAAGTGGCCAACAATCAAACCAAAACAAAACATTCAGATAACCCGTCTCAAAGACAATGATCTTTTCACCGTACATAATTTCTTCACATCTGCAGAATCAAAGGCTTTTGTTAAGGTTGCAGAGTCTATTGGGTTTGTTCACCAAGGAAGTCTTGGCCCAACAAAAGGTGAAGCTTTTAGAGACAATGATAGAATCTCTGTGAACGATCCTGTTCTTGCTGATTCTATATGGAATTCTGGGCTCAGCAAATTGTTTTCTGACATCAAAATTCGAAGGGAGGTTGCTGTTGGCTTAAACCCAAATATCAGATTCTACAGGTACAAGGTTGGTCAGCGATTTGGGAGGCATATTGATGAAAGTGTTGATCTTGAAGATGGAAAACGCACACATTATACTTTGTTGATATATTTGAGTGGCGGTTTCAAACCGAAAGCGAAAAATGATGTGAGCAGTCATCAGGATTCATCCTCAGAGCCTCTGGTTGGAGGAGAGACCGTCTTTTATGGCTCAAGGAATAGTGTTGTGGCTGAGGTGTCTCCCGTTGAAGGGATGGCTCTTCTGCACATTCATGGGCACAAATGTATGCTGCATGAAGCTCGAAATGTTACTAAGGGTATCAAGTATGTGTTTCGTTCAGATGTTGTATTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

243

Amino Acids

27.33

Weight (kDa)

9.52

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51880
fragaria_vesca FvH4_7g28750 FvH4_7g28750
malus_domestica MD01G1193900.v1.1
prunus_persica Prupe.2G287700_v2.0.a1
pyrus_communis pycom01g20420
rosa_chinensis RchiOBHm_Chr1g0376301
rosa_laevigata RLG00000026613
rosa_multiflora Rmu_ssc0000116.1_g000061
rosa_roxburghii Rroxscaffold_4G00282220
rosa_rugosa Rorug01G0395100 Rorug01G0395200 Rorug01G0395300 Rorug01G0395300 Rorug01G0395400
rosa_samantha Rh1AG406500 Rh1BG370500 Rh1CG383300 Rh1DG400300
rosa_wichuraiana Rw1G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 495
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 2 cut(s) 310, 345
AfaI GTAC 2 cut(s) 150, 398
AfiI CCNNNNNNNGG 5 cut(s) 63, 242, 560, 623, 624
AgsI TTSAA 3 cut(s) 449, 502, 623
AjnI CCWGG 1 cut(s) 62
AleI CACNNNNGTG 1 cut(s) 79
AluBI AGCT 3 cut(s) 50, 251, 674
AluI AGCT 3 cut(s) 50, 251, 674
Alw26I GTCTC 4 cut(s) 128, 252, 563, 618
AlwI GGATC 1 cut(s) 278
AoxI GGCC 2 cut(s) 83, 232
ApeKI GCWGC 1 cut(s) 664
ApoI RAATTY 2 cut(s) 310, 345
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 3 cut(s) 136, 209, 257
AsuII TTCGAA 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 654
BalI TGGCCA 1 cut(s) 85
BanII GRGCYC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 606
BbvI GCAGC 1 cut(s) 651
BccI CCATC 3 cut(s) 25, 446, 622
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 1 cut(s) 64
BcoDI GTCTC 4 cut(s) 128, 252, 563, 618
BfmI CTRYAG 2 cut(s) 168, 391
BisI GCNGC 1 cut(s) 665
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 1 cut(s) 666
Bme1390I CCNGG 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 571
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 242, 560, 623, 624
BseBI CCWGG 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 2 cut(s) 542, 633
BseLI CCNNNNNNNGG 5 cut(s) 63, 242, 560, 623, 624
BseMII CTCAG 3 cut(s) 334, 561, 597
BseRI GAGGAG 1 cut(s) 579
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 1 cut(s) 651
BsgI GTGCAG 1 cut(s) 623
BshFI GGCC 2 cut(s) 85, 234
BslI CCNNNNNNNGG 5 cut(s) 63, 242, 560, 623, 624
BsmAI GTCTC 4 cut(s) 128, 252, 563, 618
BsmBI CGTCTC 1 cut(s) 128
BsnI GGCC 2 cut(s) 85, 234
Bso31I GGTCTC 1 cut(s) 563
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 321, 654
Bsp143I GATC 3 cut(s) 136, 283, 442
Bsp1720I GCTNAGC 1 cut(s) 320
BspACI CCGC 1 cut(s) 495
BspANI GGCC 2 cut(s) 85, 234
BspCNI CTCAG 3 cut(s) 333, 560, 598
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 1 cut(s) 278
BspQI GCTCTTC 1 cut(s) 639
BspT104I TTCGAA 1 cut(s) 349
BspTNI GGTCTC 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 3 cut(s) 136, 283, 442
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 64
Bst4CI ACNGT 2 cut(s) 148, 574
Bst6I CTCTTC 3 cut(s) 17, 37, 639
BstBI TTCGAA 1 cut(s) 349
BstDEI CTNAG 4 cut(s) 320, 547, 606, 687
BstF5I GGATG 2 cut(s) 542, 633
BstKTI GATC 3 cut(s) 139, 286, 445
BstMAI GTCTC 4 cut(s) 128, 252, 563, 618
BstMBI GATC 3 cut(s) 136, 283, 442
BstNI CCWGG 1 cut(s) 64
BstSCI CCNGG 1 cut(s) 62
BstSFI CTRYAG 2 cut(s) 168, 391
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 651
BsuRI GGCC 2 cut(s) 85, 234
BtsCI GGATG 2 cut(s) 542, 633
Cfr13I GGNCC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 149, 397
CviAII CATG 3 cut(s) 647, 668, 729
CviQI GTAC 2 cut(s) 149, 397
DdeI CTNAG 4 cut(s) 320, 547, 606, 687
DpnI GATC 3 cut(s) 138, 285, 444
DpnII GATC 3 cut(s) 136, 283, 442
EaeI YGGCCR 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 17, 37, 639
EarI CTCTTC 3 cut(s) 17, 37, 639
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 563
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 3 cut(s) 650, 671, 732
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 3 cut(s) 646, 667, 728
Fnu4HI GCNGC 1 cut(s) 665
FokI GGATG 2 cut(s) 529, 640
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 665
GluI GCNGC 1 cut(s) 665
HaeIII GGCC 2 cut(s) 85, 234
Hin1II CATG 3 cut(s) 650, 671, 732
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 7 cut(s) 53, 173, 200, 270, 299, 387, 539
HphI GGTGA 3 cut(s) 136, 209, 257
Hpy166II GTNNAC 2 cut(s) 217, 280
Hpy188I TCNGA 5 cut(s) 114, 337, 386, 550, 715
Hpy188III TCNNGA 2 cut(s) 446, 536
Hpy8I GTNNAC 2 cut(s) 217, 280
HpyAV CCTTC 2 cut(s) 345, 617
HpyCH4III ACNGT 2 cut(s) 148, 574
HpyCH4V TGCA 5 cut(s) 170, 197, 640, 667, 728
HpyF3I CTNAG 4 cut(s) 320, 547, 606, 687
Hsp92II CATG 3 cut(s) 650, 671, 732
Kzo9I GATC 3 cut(s) 136, 283, 442
LguI GCTCTTC 1 cut(s) 639
LpnPI CCDG 7 cut(s) 49, 76, 300, 300, 379, 521, 542
Lsp1109I GCAGC 1 cut(s) 651
MaeIII GTNAC 1 cut(s) 682
MalI GATC 3 cut(s) 138, 285, 444
MboI GATC 3 cut(s) 136, 283, 442
MboII GAAGA 6 cut(s) 34, 40, 54, 151, 461, 626
MhlI GDGCHC 2 cut(s) 321, 654
MlsI TGGCCA 1 cut(s) 85
MluCI AATT 5 cut(s) 58, 154, 310, 326, 345
MluNI TGGCCA 1 cut(s) 85
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 541
MnlI CCTC 7 cut(s) 18, 349, 414, 556, 557, 564, 601
Mox20I TGGCCA 1 cut(s) 85
MscI TGGCCA 1 cut(s) 85
MseI TTAA 2 cut(s) 189, 371
MslI CAYNNNNRTG 3 cut(s) 79, 429, 645
Msp20I TGGCCA 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 64
MvaI CCWGG 1 cut(s) 64
NdeII GATC 3 cut(s) 136, 283, 442
NlaIII CATG 3 cut(s) 650, 671, 732
NspV TTCGAA 1 cut(s) 349
OliI CACNNNNGTG 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 639
PfeI GAWTC 6 cut(s) 53, 173, 270, 299, 387, 539
PfoI TCCNGGA 1 cut(s) 62
PkrI GCNGC 1 cut(s) 666
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 62
PspGI CCWGG 1 cut(s) 62
PspPI GGNCC 1 cut(s) 233
PstI CTGCAG 1 cut(s) 172
RsaI GTAC 2 cut(s) 150, 398
RsaNI GTAC 2 cut(s) 149, 397
RseI CAYNNNNRTG 3 cut(s) 79, 429, 645
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 2 cut(s) 189, 371
SatI GCNGC 1 cut(s) 665
Sau3AI GATC 3 cut(s) 136, 283, 442
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 64
SduI GDGCHC 2 cut(s) 321, 654
SetI ASST 9 cut(s) 52, 195, 247, 253, 360, 398, 405, 612, 676
SfcI CTRYAG 2 cut(s) 168, 391
SfuI TTCGAA 1 cut(s) 349
SmiMI CAYNNNNRTG 3 cut(s) 79, 429, 645
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
Sse9I AATT 5 cut(s) 58, 154, 310, 326, 345
SsiI CCGC 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 62
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 148, 574
TaqI TCGA 2 cut(s) 349, 676
TasI AATT 5 cut(s) 58, 154, 310, 326, 345
TfiI GAWTC 6 cut(s) 53, 173, 270, 299, 387, 539
Tru1I TTAA 2 cut(s) 189, 371
Tru9I TTAA 2 cut(s) 189, 371
TseI GCWGC 1 cut(s) 664
TspDTI ATGAA 4 cut(s) 447, 531, 635, 684
XapI RAATTY 2 cut(s) 310, 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.