pycom01g20420

Prolyl 4-hydroxylase alpha subunit homologues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011173
Physical Location & Seq
Forward (+)
18858859 .. 18860270
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20420.3

Sequence Viewer

Length: 732 bp
ATGGCTGAAACACGAACAACAAACAAGCGGAAAATGGCAGACCGGAGAGGAGAAGCAGATTCCGAGAAGACGTCCAATTGGCCGTCCATTAAACCAAAGAAATATCTTCAGATAACTCCTCTCAAAGATACCCATCTTTTTACTGTGCAGAATTTCCTGACTTCTGCAGAATCAAATGCTTTCGTTAAAGCTGCAGAGTCTATTGGATTTGTTCACCAAGGGAGTCTTGGCCCGACGAAAGGCGAAGCGTACAGAGACAATGATAGAATCTCTGTGAATGATACTGTTCTTGCAGATACAATATGGGAGTCTGGTCTTAACAAATTGTTTTCTGACATTAAAATTCGAGGGAGGACCGCTGTTGGATTAAACCCGAATATCAGATTCTATAGGTACAAGGCTGGTCAGCGCTTTGGAAGGCATATTGATGAAAGTAATGATCTTGGTGACGGAAAACGCACCCATTATACTTTGCTAATATACTTAAGTGGTGGCGGTTTCAAACCAAAAGCTAAAAGTGATACGAGCAGTCTTAAGGATTCTTCCTCAGAGCCTCTGGTTGGAGGAGAAACAGTTTTCTATGGTTCAAGGAATAGTGTTGTGGCAGAGGTGCCTCCCACTGAAGGAATGGCTCTTCTGCACATTCACGGGGACAAATGCTTGCTGCATGAAGCCCGAAACGTTACAAAGGGTATCAAGTATGTATTTCGTTCGGATGTAGTATTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_3

Protein Analysis

244

Amino Acids

26.97

Weight (kDa)

9.46

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51880
fragaria_vesca FvH4_7g28750 FvH4_7g28750
malus_domestica MD01G1193900.v1.1
prunus_persica Prupe.2G287700_v2.0.a1
pyrus_communis pycom01g20420
rosa_chinensis RchiOBHm_Chr1g0376301
rosa_laevigata RLG00000026613
rosa_multiflora Rmu_ssc0000116.1_g000061
rosa_roxburghii Rroxscaffold_4G00282220
rosa_rugosa Rorug01G0395100 Rorug01G0395200 Rorug01G0395300 Rorug01G0395300 Rorug01G0395400
rosa_samantha Rh1AG406500 Rh1BG370500 Rh1CG383300 Rh1DG400300
rosa_wichuraiana Rw1G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 610
AciI CCGC 3 cut(s) 28, 357, 495
AclI AACGTT 1 cut(s) 681
AcoI YGGCCR 1 cut(s) 80
AcsI RAATTY 2 cut(s) 151, 342
AcuI CTGAAG 2 cut(s) 92, 642
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 2 cut(s) 251, 395
AfeI AGCGCT 1 cut(s) 410
AfiI CCNNNNNNNGG 3 cut(s) 239, 560, 623
AflII CTTAAG 2 cut(s) 484, 533
AgsI TTSAA 2 cut(s) 502, 588
AluBI AGCT 2 cut(s) 191, 512
AluI AGCT 2 cut(s) 191, 512
Alw26I GTCTC 1 cut(s) 249
Aor51HI AGCGCT 1 cut(s) 410
AoxI GGCC 2 cut(s) 80, 229
ApeKI GCWGC 2 cut(s) 191, 664
ApoI RAATTY 2 cut(s) 151, 342
AspLEI GCGC 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 230, 354
AsuHPI GGTGA 2 cut(s) 206, 458
AvaII GGWCC 1 cut(s) 354
BanI GGYRCC 1 cut(s) 610
BbsI GAAGAC 1 cut(s) 74
BbvI GCAGC 2 cut(s) 178, 651
BccI CCATC 1 cut(s) 141
BceAI ACGGC 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 249
BfmI CTRYAG 3 cut(s) 165, 192, 388
BfoI RGCGCY 1 cut(s) 412
BfrI CTTAAG 2 cut(s) 484, 533
BisI GCNGC 2 cut(s) 192, 665
BlsI GCNGC 2 cut(s) 193, 666
Bme18I GGWCC 1 cut(s) 354
BmgT120I GGNCC 2 cut(s) 230, 354
BmiI GGNNCC 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 558, 588
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 560, 623
Bse8I GATNNNNATC 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 1 cut(s) 721
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 3 cut(s) 239, 560, 623
BseMII CTCAG 1 cut(s) 561
BseRI GAGGAG 3 cut(s) 63, 108, 579
BseXI GCAGC 2 cut(s) 178, 651
BsgI GTGCAG 2 cut(s) 167, 623
BshFI GGCC 2 cut(s) 82, 231
BshNI GGYRCC 1 cut(s) 610
BsiSI CCGG 1 cut(s) 43
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 3 cut(s) 239, 560, 623
BsmAI GTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 2 cut(s) 82, 231
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 3 cut(s) 28, 357, 495
BspANI GGCC 2 cut(s) 82, 231
BspCNI CTCAG 1 cut(s) 560
BspLI GGNNCC 1 cut(s) 612
BspMAI CTGCAG 2 cut(s) 169, 196
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 610
BspTI CTTAAG 2 cut(s) 484, 533
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 439
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 217
Bst4CI ACNGT 3 cut(s) 145, 286, 574
Bst6I CTCTTC 1 cut(s) 639
BstACI GRCGYC 1 cut(s) 71
BstAFI CTTAAG 2 cut(s) 484, 533
BstC8I GCNNGC 1 cut(s) 662
BstDEI CTNAG 1 cut(s) 547
BstF5I GGATG 1 cut(s) 721
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 1 cut(s) 442
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 1 cut(s) 439
BstSFI CTRYAG 3 cut(s) 165, 192, 388
BstV1I GCAGC 2 cut(s) 178, 651
BstV2I GAAGAC 1 cut(s) 74
BsuRI GGCC 2 cut(s) 82, 231
BtsCI GGATG 1 cut(s) 721
BtsIMutI CAGTG 1 cut(s) 618
Cac8I GCNNGC 1 cut(s) 662
CfoI GCGC 1 cut(s) 411
Cfr13I GGNCC 2 cut(s) 230, 354
Csp6I GTAC 2 cut(s) 250, 394
CviAII CATG 1 cut(s) 668
CviJI RGCY 9 cut(s) 5, 82, 191, 231, 401, 512, 553, 632, 674
CviKI_1 RGCY 9 cut(s) 5, 82, 191, 231, 401, 512, 553, 632, 674
CviQI GTAC 2 cut(s) 250, 394
DdeI CTNAG 1 cut(s) 547
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 80
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 354
Eco47III AGCGCT 1 cut(s) 410
Eco57I CTGAAG 2 cut(s) 92, 642
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 1 cut(s) 671
FaiI YATR 8 cut(s) 304, 390, 423, 468, 481, 582, 669, 702
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 665
FatI CATG 1 cut(s) 667
Fnu4HI GCNGC 2 cut(s) 192, 665
FokI GGATG 1 cut(s) 728
Fsp4HI GCNGC 2 cut(s) 192, 665
GlaI GCGC 1 cut(s) 410
GluI GCNGC 2 cut(s) 192, 665
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 2 cut(s) 82, 231
HapII CCGG 1 cut(s) 43
HhaI GCGC 1 cut(s) 411
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 1 cut(s) 671
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HinfI GANTC 8 cut(s) 59, 170, 197, 223, 267, 308, 384, 539
HpaII CCGG 1 cut(s) 43
HphI GGTGA 2 cut(s) 206, 458
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 6 cut(s) 64, 111, 334, 383, 550, 715
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 2 cut(s) 411, 617
HpyCH4III ACNGT 3 cut(s) 145, 286, 574
HpyCH4IV ACGT 2 cut(s) 71, 681
HpyCH4V TGCA 6 cut(s) 148, 167, 194, 293, 640, 667
HpyF3I CTNAG 1 cut(s) 547
HpySE526I ACGT 2 cut(s) 71, 681
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 1 cut(s) 671
HspAI GCGC 1 cut(s) 409
Kzo9I GATC 1 cut(s) 439
LguI GCTCTTC 1 cut(s) 639
LpnPI CCDG 5 cut(s) 56, 170, 297, 387, 542
Lsp1109I GCAGC 2 cut(s) 178, 651
MaeII ACGT 2 cut(s) 71, 681
MaeIII GTNAC 2 cut(s) 446, 682
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 4 cut(s) 79, 98, 534, 626
MfeI CAATTG 1 cut(s) 76
MluCI AATT 4 cut(s) 76, 151, 323, 342
MlyI GAGTC 3 cut(s) 206, 232, 317
MmeI TCCRAC 2 cut(s) 343, 541
MnlI CCTC 9 cut(s) 41, 129, 341, 345, 556, 557, 564, 601, 624
MseI TTAA 7 cut(s) 90, 186, 318, 339, 368, 485, 534
MslI CAYNNNNRTG 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 359
MspCI CTTAAG 2 cut(s) 484, 533
MspI CCGG 1 cut(s) 43
MunI CAATTG 1 cut(s) 76
NdeII GATC 1 cut(s) 439
NlaIII CATG 1 cut(s) 671
NlaIV GGNNCC 1 cut(s) 612
NmuCI GTSAC 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 639
PfeI GAWTC 5 cut(s) 59, 170, 267, 384, 539
PkrI GCNGC 2 cut(s) 193, 666
PleI GAGTC 3 cut(s) 205, 231, 316
PpsI GAGTC 3 cut(s) 205, 231, 316
Psp1406I AACGTT 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 612
PspPI GGNCC 2 cut(s) 230, 354
PstI CTGCAG 2 cut(s) 169, 196
RsaI GTAC 2 cut(s) 251, 395
RsaNI GTAC 2 cut(s) 250, 394
RseI CAYNNNNRTG 1 cut(s) 426
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 7 cut(s) 90, 186, 318, 339, 368, 485, 534
SatI GCNGC 2 cut(s) 192, 665
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 2 cut(s) 230, 354
SchI GAGTC 3 cut(s) 206, 232, 317
SetI ASST 6 cut(s) 74, 193, 395, 514, 612, 684
SfcI CTRYAG 3 cut(s) 165, 192, 388
SinI GGWCC 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 2 cut(s) 484, 533
SmoI CTYRAG 2 cut(s) 484, 533
Sse9I AATT 4 cut(s) 76, 151, 323, 342
SsiI CCGC 3 cut(s) 28, 357, 495
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 3 cut(s) 145, 286, 574
TaiI ACGT 2 cut(s) 74, 684
TaqI TCGA 1 cut(s) 346
TasI AATT 4 cut(s) 76, 151, 323, 342
TfiI GAWTC 5 cut(s) 59, 170, 267, 384, 539
Tru1I TTAA 7 cut(s) 90, 186, 318, 339, 368, 485, 534
Tru9I TTAA 7 cut(s) 90, 186, 318, 339, 368, 485, 534
TscAI CASTG 1 cut(s) 625
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 2 cut(s) 191, 664
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 2 cut(s) 444, 684
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 1 cut(s) 625
Vha464I CTTAAG 2 cut(s) 484, 533
VpaK11BI GGWCC 1 cut(s) 354
XapI RAATTY 2 cut(s) 151, 342
XcmI CCANNNNNNNNNTGG 2 cut(s) 224, 625
ZraI GACGTC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.