Prupe.2G309700_v2.0.a1

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0014214
Physical Location & Seq
Forward (+)
29336859 .. 29341329
4471 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G309700.1

Sequence Viewer

Length: 1302 bp
ATGTTCCAACCAAACAACCAGATTTCAGATCATACAAAGTCATTATCTATTCAAGATTCAACAGCATTGATGCAAGTGGACGAAAAAGAAACTAGTCTAGTTATGAAGGATGGGATGCAAAATGGAGGATTCGCGCAAGCAGAATCCATCATCTACTACACTAAGAAACTTCAAGATGATTTACAAATGATGGGGATGAAAATTAAACAGCATGAAGACAACATAAAACTTCTGAAGTCTCAGAGAAACAAATTAGATGACTCTATTCTCGATTTGCAAGTTATTCTAGGGAAGTATCATACCTCAACTGCATCTAAAATCGAAAATGAAGACCATTCCCACTGTAAAAGTGAGGAGGAAACAACCAAAAAGATTCTTCAGCGTGAGAAGTCTGCTGCAGGCATTTTATGGCAGTTGAAAACTCGTCATGGCACTCAGGCTGCCCATCTTACCTTGACCAAGGATGTCCTGGGTATTGTTGCAATGCTTGGCAAAGTAGAGGATGATAATCTTAGCAGGCTTTTATCAGAATACTTAGGAATCGATACTATGTTGTCAATTGTCTGTAAGACTTATGAAGGTGTCAAAGCTTTAGAAACGTATGACAATGAAGGCTGCATAAAGAAAAGTTCTGGTCTTCATGGGCTTGGTGCTTCTATTGGAAGGACATTAGAAGGCCGTTTTCAGGTCATTTGTCTTGATAATTTAAGACCATATGCTGGTGAATTTGTACCTGATGACCCACAGAGGAGGCTTGATCTTCTGAAGCCAAGGTTACCTAATGGGGAATGCCCACCCGGTTTTCTTGGTTATGCAGTAAATATGATCCATGTGGATAGCACAAGCTTATTTTGTGTCACAGCTAGTGGGCATGGCCTGAGAGAGACTCTATTTTATAACCTCTTCTTTCGCTTGCAAATATATAAAACAAGGGCAGATATGGTTCCTGCACTTCCTTGTATAAGTGATGGAGCCATTTCTTTAGATGGCGGGATGATTAGGTCAACTGGTGTGTTTTCTCTTGGCAACCGGGAAGATGTAGATGTGAGATTTCCTAAACTGTCAGTGACATCAAGCCTACCCGAAACCTATCTTGATTCTGAAAGACAAATCAATGAGTTGAAGTGGAAAAAAGAAAAAATGCAGGAAGATATGAAGAGAGAACAAGCATTATTGGACAACGCGAAGTTCAATTTTGACAGAAAGAAGCAAGATTTTCTGAAATTCCTGGCTGATAGCTCATCATATGCAACTCAGCATCAATTTCAGGCAGCAGCGCAAAGCCGAGTGACCTCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

434

Amino Acids

48.79

Weight (kDa)

6.61

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 897
AccB7I CCANNNNNTGG 1 cut(s) 719
AccII CGCG 2 cut(s) 134, 1184
AciI CCGC 1 cut(s) 990
AclWI GGATC 1 cut(s) 820
AcsI RAATTY 2 cut(s) 725, 1223
AcuI CTGAAG 3 cut(s) 254, 362, 785
AfaI GTAC 1 cut(s) 732
AfiI CCNNNNNNNGG 2 cut(s) 685, 719
AgsI TTSAA 6 cut(s) 53, 60, 173, 418, 1123, 1192
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 2 cut(s) 468, 1227
AluBI AGCT 4 cut(s) 590, 846, 863, 1239
AluI AGCT 4 cut(s) 590, 846, 863, 1239
Alw26I GTCTC 2 cut(s) 243, 878
AlwI GGATC 1 cut(s) 820
AoxI GGCC 2 cut(s) 676, 874
ApeKI GCWGC 5 cut(s) 395, 440, 615, 1271, 1274
ApoI RAATTY 2 cut(s) 725, 1223
AspLEI GCGC 2 cut(s) 136, 1279
AsuC2I CCSGG 2 cut(s) 798, 1031
AsuHPI GGTGA 1 cut(s) 734
BbsI GAAGAC 3 cut(s) 222, 336, 629
BbvI GCAGC 5 cut(s) 382, 427, 602, 1283, 1286
BccI CCATC 6 cut(s) 104, 155, 184, 453, 962, 980
BceAI ACGGC 1 cut(s) 663
BciT130I CCWGG 2 cut(s) 470, 1229
BcnI CCSGG 2 cut(s) 798, 1031
BcoDI GTCTC 2 cut(s) 243, 878
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 4 cut(s) 93, 98, 287, 864
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 5 cut(s) 396, 441, 616, 1272, 1275
BlsI GCNGC 5 cut(s) 397, 442, 617, 1273, 1276
Bme1390I CCNGG 4 cut(s) 470, 798, 1031, 1229
BmiI GGNNCC 2 cut(s) 945, 973
BmrFI CCNGG 4 cut(s) 470, 798, 1031, 1229
BmsI GCATC 4 cut(s) 60, 105, 320, 1267
BpiI GAAGAC 3 cut(s) 222, 336, 629
BplI GAGNNNNNCTC 2 cut(s) 871, 903
BpuEI CTTGAG 1 cut(s) 1279
BpuMI CCSGG 2 cut(s) 798, 1031
Bsa29I ATCGAT 1 cut(s) 543
BsaBI GATNNNNATC 1 cut(s) 507
BsaJI CCNNGG 3 cut(s) 459, 469, 770
Bsc4I CCNNNNNNNGG 2 cut(s) 685, 719
Bse1I ACTGG 1 cut(s) 1012
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 2 cut(s) 470, 1229
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 3 cut(s) 459, 469, 770
BseGI GGATG 6 cut(s) 115, 120, 201, 469, 508, 999
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 685, 719
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 4 cut(s) 254, 449, 869, 1268
BseNI ACTGG 1 cut(s) 1012
BseRI GAGGAG 2 cut(s) 368, 763
BseXI GCAGC 5 cut(s) 382, 427, 602, 1283, 1286
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 2 cut(s) 134, 1184
BshFI GGCC 2 cut(s) 678, 876
BshVI ATCGAT 1 cut(s) 543
BsiSI CCGG 2 cut(s) 798, 1030
BslI CCNNNNNNNGG 2 cut(s) 685, 719
BsmAI GTCTC 2 cut(s) 243, 878
BsmI GAATGC 1 cut(s) 794
BsnI GGCC 2 cut(s) 678, 876
Bsp143I GATC 3 cut(s) 28, 757, 825
BspACI CCGC 1 cut(s) 990
BspANI GGCC 2 cut(s) 678, 876
BspCNI CTCAG 4 cut(s) 253, 448, 870, 1267
BspDI ATCGAT 1 cut(s) 543
BspFNI CGCG 2 cut(s) 134, 1184
BspLI GGNNCC 2 cut(s) 945, 973
BspMAI CTGCAG 1 cut(s) 400
BspPI GGATC 1 cut(s) 820
BsrDI GCAATG 1 cut(s) 489
BsrI ACTGG 1 cut(s) 1012
BssECI CCNNGG 3 cut(s) 459, 469, 770
BssMI GATC 3 cut(s) 28, 757, 825
BssT1I CCWWGG 2 cut(s) 459, 770
Bst2UI CCWGG 2 cut(s) 470, 1229
Bst4CI ACNGT 2 cut(s) 344, 1062
Bst6I CTCTTC 2 cut(s) 908, 1151
BstC8I GCNNGC 4 cut(s) 138, 400, 518, 914
BstDEI CTNAG 7 cut(s) 162, 240, 435, 512, 535, 878, 1254
BstEII GGTNACC 1 cut(s) 774
BstF5I GGATG 6 cut(s) 115, 120, 201, 469, 508, 999
BstFNI CGCG 2 cut(s) 134, 1184
BstHHI GCGC 2 cut(s) 136, 1279
BstKTI GATC 3 cut(s) 31, 760, 828
BstMAI GTCTC 2 cut(s) 243, 878
BstMBI GATC 3 cut(s) 28, 757, 825
BstNI CCWGG 2 cut(s) 470, 1229
BstPI GGTNACC 1 cut(s) 774
BstSCI CCNGG 4 cut(s) 468, 796, 1029, 1227
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 2 cut(s) 134, 1184
BstV1I GCAGC 5 cut(s) 382, 427, 602, 1283, 1286
BstV2I GAAGAC 3 cut(s) 222, 336, 629
Bsu15I ATCGAT 1 cut(s) 543
BsuRI GGCC 2 cut(s) 678, 876
BsuTUI ATCGAT 1 cut(s) 543
BtsCI GGATG 6 cut(s) 115, 120, 201, 469, 508, 999
BtsIMutI CAGTG 2 cut(s) 340, 1071
Cac8I GCNNGC 4 cut(s) 138, 400, 518, 914
CfoI GCGC 2 cut(s) 136, 1279
ClaI ATCGAT 1 cut(s) 543
Csp6I GTAC 1 cut(s) 731
CviAII CATG 5 cut(s) 212, 428, 641, 830, 872
CviQI GTAC 1 cut(s) 731
DdeI CTNAG 7 cut(s) 162, 240, 435, 512, 535, 878, 1254
DpnI GATC 3 cut(s) 30, 759, 827
DpnII GATC 3 cut(s) 28, 757, 825
Eam1104I CTCTTC 2 cut(s) 908, 1151
EarI CTCTTC 2 cut(s) 908, 1151
Eco130I CCWWGG 2 cut(s) 459, 770
Eco57I CTGAAG 3 cut(s) 254, 362, 785
Eco91I GGTNACC 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 774
EcoRII CCWGG 2 cut(s) 468, 1227
EcoT14I CCWWGG 2 cut(s) 459, 770
ErhI CCWWGG 2 cut(s) 459, 770
FaeI CATG 5 cut(s) 215, 431, 644, 833, 875
FatI CATG 5 cut(s) 211, 427, 640, 829, 871
FauI CCCGC 1 cut(s) 983
FauNDI CATATG 2 cut(s) 715, 1246
Fnu4HI GCNGC 5 cut(s) 396, 441, 616, 1272, 1275
FokI GGATG 6 cut(s) 122, 127, 208, 476, 515, 1006
Fsp4HI GCNGC 5 cut(s) 396, 441, 616, 1272, 1275
FspBI CTAG 4 cut(s) 93, 98, 287, 864
GlaI GCGC 2 cut(s) 135, 1278
GluI GCNGC 5 cut(s) 396, 441, 616, 1272, 1275
HaeIII GGCC 2 cut(s) 678, 876
HapII CCGG 2 cut(s) 798, 1030
HhaI GCGC 2 cut(s) 136, 1279
Hin1II CATG 5 cut(s) 215, 431, 644, 833, 875
Hin6I GCGC 2 cut(s) 134, 1277
HinP1I GCGC 2 cut(s) 134, 1277
HincII GTYRAC 1 cut(s) 1005
HindII GTYRAC 1 cut(s) 1005
HindIII AAGCTT 2 cut(s) 588, 844
HinfI GANTC 8 cut(s) 56, 129, 143, 260, 373, 540, 886, 1097
HpaII CCGG 2 cut(s) 798, 1030
HphI GGTGA 1 cut(s) 734
Hpy166II GTNNAC 2 cut(s) 79, 1005
Hpy188I TCNGA 7 cut(s) 28, 234, 243, 529, 765, 1102, 1221
Hpy188III TCNNGA 5 cut(s) 53, 173, 269, 698, 1094
Hpy8I GTNNAC 2 cut(s) 79, 1005
HpyAV CCTTC 5 cut(s) 100, 572, 605, 657, 668
HpyCH4III ACNGT 2 cut(s) 344, 1062
HpyCH4IV ACGT 1 cut(s) 599
HpyF3I CTNAG 7 cut(s) 162, 240, 435, 512, 535, 878, 1254
HpySE526I ACGT 1 cut(s) 599
Hsp92II CATG 5 cut(s) 215, 431, 644, 833, 875
HspAI GCGC 2 cut(s) 134, 1277
Kzo9I GATC 3 cut(s) 28, 757, 825
LmnI GCTCC 1 cut(s) 971
Lsp1109I GCAGC 5 cut(s) 382, 427, 602, 1283, 1286
LweI GCATC 4 cut(s) 60, 105, 320, 1267
MaeI CTAG 4 cut(s) 93, 98, 287, 864
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 4 cut(s) 774, 856, 1066, 1288
MalI GATC 3 cut(s) 30, 759, 827
MboI GATC 3 cut(s) 28, 757, 825
MboII GAAGA 9 cut(s) 227, 341, 368, 629, 752, 895, 1046, 1160, 1168
MfeI CAATTG 1 cut(s) 558
MluCI AATT 8 cut(s) 201, 251, 558, 703, 725, 1192, 1223, 1262
MlyI GAGTC 2 cut(s) 254, 880
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 8 cut(s) 119, 313, 346, 349, 493, 741, 744, 911
MseI TTAA 2 cut(s) 204, 707
MspI CCGG 2 cut(s) 798, 1030
MspR9I CCNGG 4 cut(s) 470, 798, 1031, 1229
MunI CAATTG 1 cut(s) 558
Mva1269I GAATGC 1 cut(s) 794
MvaI CCWGG 2 cut(s) 470, 1229
MvnI CGCG 2 cut(s) 134, 1184
NciI CCSGG 2 cut(s) 798, 1031
NdeI CATATG 2 cut(s) 715, 1246
NdeII GATC 3 cut(s) 28, 757, 825
NlaIII CATG 5 cut(s) 215, 431, 644, 833, 875
NlaIV GGNNCC 2 cut(s) 945, 973
NmuCI GTSAC 3 cut(s) 856, 1066, 1288
PctI GAATGC 1 cut(s) 794
PfeI GAWTC 6 cut(s) 56, 129, 143, 373, 540, 1097
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 5 cut(s) 397, 442, 617, 1273, 1276
PleI GAGTC 2 cut(s) 254, 880
PpsI GAGTC 2 cut(s) 254, 880
PsiI TTATAA 1 cut(s) 897
Psp6I CCWGG 2 cut(s) 468, 1227
PspEI GGTNACC 1 cut(s) 774
PspGI CCWGG 2 cut(s) 468, 1227
PspN4I GGNNCC 2 cut(s) 945, 973
PstI CTGCAG 1 cut(s) 400
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
SaqAI TTAA 2 cut(s) 204, 707
SatI GCNGC 5 cut(s) 396, 441, 616, 1272, 1275
Sau3AI GATC 3 cut(s) 28, 757, 825
SchI GAGTC 2 cut(s) 254, 880
ScrFI CCNGG 4 cut(s) 470, 798, 1031, 1229
SfaNI GCATC 4 cut(s) 60, 105, 320, 1267
SfcI CTRYAG 1 cut(s) 396
SmlI CTYRAG 1 cut(s) 1294
SmoI CTYRAG 1 cut(s) 1294
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 8 cut(s) 201, 251, 558, 703, 725, 1192, 1223, 1262
SsiI CCGC 1 cut(s) 990
SspMI CTAG 4 cut(s) 93, 98, 287, 864
StyD4I CCNGG 4 cut(s) 468, 796, 1029, 1227
StyI CCWWGG 2 cut(s) 459, 770
TaaI ACNGT 2 cut(s) 344, 1062
TaiI ACGT 1 cut(s) 602
TaqI TCGA 3 cut(s) 270, 321, 543
TasI AATT 8 cut(s) 201, 251, 558, 703, 725, 1192, 1223, 1262
TfiI GAWTC 6 cut(s) 56, 129, 143, 373, 540, 1097
Tru1I TTAA 2 cut(s) 204, 707
Tru9I TTAA 2 cut(s) 204, 707
TscAI CASTG 2 cut(s) 347, 1071
TseFI GTSAC 3 cut(s) 856, 1066, 1288
TseI GCWGC 5 cut(s) 395, 440, 615, 1271, 1274
Tsp45I GTSAC 3 cut(s) 856, 1066, 1288
TspDTI ATGAA 8 cut(s) 119, 212, 228, 342, 591, 624, 629, 1169
TspRI CASTG 2 cut(s) 347, 1071
Van91I CCANNNNNTGG 1 cut(s) 719
XapI RAATTY 2 cut(s) 725, 1223
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XspI CTAG 4 cut(s) 93, 98, 287, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.