Rh1CG416400

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0014214
Physical Location & Seq
Forward (+)
67772915 .. 67777103
4189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG416400.1

Sequence Viewer

Length: 1392 bp
ATGTTCCAACCCAACAACAACAACAACAACAACCAGCTTCCAATTAAGTCATTACCTATTCAAGATCCATCAGCACCGATGCAAGTGGATAAAAAAGAAACTAGCATAGTCTTGAAGGATGAAACGAAAAACGGAGGATTCGCGCAAGCAGAATCTATCATCTTCTACACTAAGAGACTTCAAGATGATTTGCATGAGATGGGGTTGAAAGTAAAACAGCATGAGGAGGACATGAAACTTCTGAGGTCTCAGAAAAACAAATTAGAAGAATCTATTCTTGATCTGCAAGTTATGTTAGGCAACTATCATTCCTCAACCACAACTACTACTGCAAATGATAACCATAGTCAGGAGGAAACAATTAAAAAGATTCTTCAGTGCGAGAAGTCAGCTGCAGGCATTTTATGGCAGCTGAAAGCTCGTCATGGTAATCAGGCTGCAGATCTTCCCCTGACCAAGGATATTGTGGGTATTGTTGCGATGCTTGGGAAAGTAGATGATGATAATCTTAGCAGGCTTTTGTCAGAGTACTTGGGACTGGAGACTATGTTAGCAATTGTCTGCAAGACTTTTGATGGTGTTAGAGCTCTAGAATTGTATGACAACGAAGGTTGCATAAACCTGAATTCCGGTCTTCACGGGCTTGGTACTTCTATTGGGAGGAAATTGGAAGGTCGGTTTCTGGTCATTTGTCTTGAAAATTTAAGGTATATCTCCTGTATGCTGTCATTTGTCAGGCAATCTGCACAGGTATTGATTTATGCACTCAAAAACTTTTTCTCTTTCTTCTGCAGACCATATGCTGGTGAGTTTGTACATAATGACCCCCAACGGAGGCTTGATATTCTAAAACCAAGGTTGCTGAATGGGGAGTGTCCACCTGGATTTCTTGGCTATGCTGTAAATATGATCAATGTGGATAGCACAAATTTATTTTGTCTCACAGCCAGTGGACACGGCCTGAGAGAGACTCTACTTTATAGCCTCTTTTCTCGCTTGCAAGTGTATAAAACAAGAGCAGATATGGTATCTGCTCTTCCTTGTATAACTGATGGAGCCATTTCTTTAGATGGTGGGATGATTCAGAGGACTGGTGTGTTTTCCCTTGGCAACAGGGAAGACGTAGGTGTGAATTTTCCAAAGCTCTCAGTGGCATCAGGCCTGCCCGAAAGCTATCTAGAAACAGAAAGACAAATCAATGAGTCGAAATGGAGAAAAGAAAAATTGATTGAAGATACGAAGAGAGTACAGGCATTATGGGACAATGCAAAATTCAATTTTGACAGAAAGAAAAAAGAGTTCCTCAAGTTCCTGGCTGATAGTTCATCTTATGCAACTCAGGCAACTCAGCATCAAATTATGGGGGCGCAGAATAGATTTACCTCGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

463

Amino Acids

52.11

Weight (kDa)

7.56

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 803
AccII CGCG 1 cut(s) 143
AclWI GGATC 1 cut(s) 59
AcsI RAATTY 5 cut(s) 625, 700, 928, 1132, 1271
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 4 cut(s) 530, 649, 816, 1248
AfiI CCNNNNNNNGG 4 cut(s) 349, 457, 803, 834
AgsI TTSAA 7 cut(s) 62, 115, 182, 208, 698, 1232, 1276
AjnI CCWGG 2 cut(s) 880, 1311
AluBI AGCT 8 cut(s) 37, 392, 412, 419, 587, 1144, 1173, 1389
AluI AGCT 8 cut(s) 37, 392, 412, 419, 587, 1144, 1173, 1389
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 5 cut(s) 169, 252, 536, 944, 962
AlwI GGATC 1 cut(s) 59
Ama87I CYCGRG 1 cut(s) 1384
AoxI GGCC 2 cut(s) 958, 1159
ApeKI GCWGC 3 cut(s) 392, 409, 437
ApoI RAATTY 5 cut(s) 625, 700, 928, 1132, 1271
Asp700I GAANNNNTTC 1 cut(s) 273
AspLEI GCGC 2 cut(s) 145, 1369
AsuHPI GGTGA 1 cut(s) 818
AvaI CYCGRG 1 cut(s) 1384
BanII GRGCYC 1 cut(s) 589
BarI GAAGNNNNNNTAC 2 cut(s) 1020, 1052
BbsI GAAGAC 2 cut(s) 626, 1125
Bbv12I GWGCWC 1 cut(s) 589
BbvI GCAGC 3 cut(s) 379, 421, 424
BccI CCATC 5 cut(s) 76, 193, 569, 1046, 1064
BceAI ACGGC 1 cut(s) 973
BciT130I CCWGG 2 cut(s) 882, 1313
BclI TGATCA 1 cut(s) 909
BcoDI GTCTC 5 cut(s) 169, 252, 536, 944, 962
BfaI CTAG 3 cut(s) 102, 590, 1178
BfmI CTRYAG 3 cut(s) 393, 438, 790
BglII AGATCT 1 cut(s) 442
BisI GCNGC 3 cut(s) 393, 410, 438
BlsI GCNGC 3 cut(s) 394, 411, 439
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 882, 1313
BmeT110I CYCGRG 1 cut(s) 1384
BmiI GGNNCC 1 cut(s) 1057
BmrFI CCNGG 2 cut(s) 882, 1313
BmsI GCATC 4 cut(s) 69, 471, 1163, 1360
BpiI GAAGAC 2 cut(s) 626, 1125
BplI GAGNNNNNCTC 2 cut(s) 955, 987
BpmI CTGGAG 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 1289
BsaBI GATNNNNATC 1 cut(s) 504
BsaI GGTCTC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 456, 854, 1105
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 4 cut(s) 349, 457, 803, 834
Bse1I ACTGG 3 cut(s) 543, 948, 1096
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 2 cut(s) 882, 1313
BseDI CCNNGG 3 cut(s) 456, 854, 1105
BseGI GGATG 2 cut(s) 124, 1083
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 4 cut(s) 349, 457, 803, 834
BseMII CTCAG 6 cut(s) 233, 263, 953, 1161, 1352, 1361
BseNI ACTGG 3 cut(s) 543, 948, 1096
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 3 cut(s) 379, 421, 424
BsgI GTGCAG 1 cut(s) 729
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 2 cut(s) 960, 1161
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 1 cut(s) 1384
BsiSI CCGG 1 cut(s) 630
BslFI GGGAC 2 cut(s) 549, 1274
BslI CCNNNNNNNGG 4 cut(s) 349, 457, 803, 834
BsmAI GTCTC 5 cut(s) 169, 252, 536, 944, 962
BsmFI GGGAC 2 cut(s) 549, 1274
BsnI GGCC 2 cut(s) 960, 1161
Bso31I GGTCTC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 1384
Bsp1286I GDGCHC 1 cut(s) 589
Bsp1407I TGTACA 1 cut(s) 814
Bsp143I GATC 4 cut(s) 64, 280, 442, 909
BspANI GGCC 2 cut(s) 960, 1161
BspCNI CTCAG 6 cut(s) 234, 262, 954, 1160, 1351, 1360
BspFNI CGCG 1 cut(s) 143
BspLI GGNNCC 1 cut(s) 1057
BspMAI CTGCAG 3 cut(s) 397, 442, 794
BspPI GGATC 1 cut(s) 59
BspQI GCTCTTC 1 cut(s) 1041
BspTNI GGTCTC 1 cut(s) 252
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 3 cut(s) 543, 948, 1096
BssECI CCNNGG 3 cut(s) 456, 854, 1105
BssMI GATC 4 cut(s) 64, 280, 442, 909
BssT1I CCWWGG 3 cut(s) 456, 854, 1105
Bst2UI CCWGG 2 cut(s) 882, 1313
Bst6I CTCTTC 2 cut(s) 1041, 1235
BstAUI TGTACA 1 cut(s) 814
BstC8I GCNNGC 5 cut(s) 147, 397, 515, 998, 1163
BstDEI CTNAG 8 cut(s) 171, 242, 249, 509, 962, 1147, 1338, 1347
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 2 cut(s) 124, 1083
BstFNI CGCG 1 cut(s) 143
BstHHI GCGC 2 cut(s) 145, 1369
BstKTI GATC 4 cut(s) 67, 283, 445, 912
BstMAI GTCTC 5 cut(s) 169, 252, 536, 944, 962
BstMBI GATC 4 cut(s) 64, 280, 442, 909
BstMWI GCNNNNNNNGC 1 cut(s) 1340
BstNI CCWGG 2 cut(s) 882, 1313
BstSCI CCNGG 2 cut(s) 880, 1311
BstSFI CTRYAG 3 cut(s) 393, 438, 790
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 3 cut(s) 379, 421, 424
BstV2I GAAGAC 2 cut(s) 626, 1125
BstX2I RGATCY 2 cut(s) 64, 442
BstYI RGATCY 2 cut(s) 64, 442
BsuRI GGCC 2 cut(s) 960, 1161
BtgZI GCGATG 1 cut(s) 494
BtsCI GGATG 2 cut(s) 124, 1083
BtsIMutI CAGTG 3 cut(s) 383, 955, 1155
Cac8I GCNNGC 5 cut(s) 147, 397, 515, 998, 1163
CfoI GCGC 2 cut(s) 145, 1369
Csp6I GTAC 4 cut(s) 529, 648, 815, 1247
CviAII CATG 4 cut(s) 194, 221, 232, 425
CviQI GTAC 4 cut(s) 529, 648, 815, 1247
DdeI CTNAG 8 cut(s) 171, 242, 249, 509, 962, 1147, 1338, 1347
DpnI GATC 4 cut(s) 66, 282, 444, 911
DpnII GATC 4 cut(s) 64, 280, 442, 909
Eam1104I CTCTTC 2 cut(s) 1041, 1235
EarI CTCTTC 2 cut(s) 1041, 1235
Ecl136II GAGCTC 1 cut(s) 587
Eco130I CCWWGG 3 cut(s) 456, 854, 1105
Eco147I AGGCCT 1 cut(s) 1161
Eco24I GRGCYC 1 cut(s) 589
Eco31I GGTCTC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 359
Eco88I CYCGRG 1 cut(s) 1384
EcoICRI GAGCTC 1 cut(s) 587
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 2 cut(s) 880, 1311
EcoT14I CCWWGG 3 cut(s) 456, 854, 1105
EcoT38I GRGCYC 1 cut(s) 589
ErhI CCWWGG 3 cut(s) 456, 854, 1105
FaeI CATG 4 cut(s) 197, 224, 235, 428
FaqI GGGAC 2 cut(s) 549, 1274
FatI CATG 4 cut(s) 193, 220, 231, 424
FauNDI CATATG 1 cut(s) 799
FbaI TGATCA 1 cut(s) 909
Fnu4HI GCNGC 3 cut(s) 393, 410, 438
FokI GGATG 2 cut(s) 131, 1090
FriOI GRGCYC 1 cut(s) 589
Fsp4HI GCNGC 3 cut(s) 393, 410, 438
FspBI CTAG 3 cut(s) 102, 590, 1178
GlaI GCGC 2 cut(s) 144, 1368
GluI GCNGC 3 cut(s) 393, 410, 438
GsuI CTGGAG 1 cut(s) 560
HaeIII GGCC 2 cut(s) 960, 1161
HapII CCGG 1 cut(s) 630
HhaI GCGC 2 cut(s) 145, 1369
Hin1II CATG 4 cut(s) 197, 224, 235, 428
Hin6I GCGC 2 cut(s) 143, 1367
HinP1I GCGC 2 cut(s) 143, 1367
HinfI GANTC 7 cut(s) 138, 152, 269, 370, 970, 1081, 1202
HpaII CCGG 1 cut(s) 630
HphI GGTGA 1 cut(s) 818
Hpy166II GTNNAC 2 cut(s) 878, 953
Hpy188I TCNGA 4 cut(s) 243, 252, 526, 1086
Hpy188III TCNNGA 8 cut(s) 62, 112, 182, 278, 350, 590, 695, 1178
Hpy8I GTNNAC 2 cut(s) 878, 953
HpyAV CCTTC 3 cut(s) 109, 602, 665
HpyCH4IV ACGT 1 cut(s) 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 1340
HpyF3I CTNAG 8 cut(s) 171, 242, 249, 509, 962, 1147, 1338, 1347
HpySE526I ACGT 1 cut(s) 1122
Hsp92II CATG 4 cut(s) 197, 224, 235, 428
HspAI GCGC 2 cut(s) 143, 1367
Ksp22I TGATCA 1 cut(s) 909
Kzo9I GATC 4 cut(s) 64, 280, 442, 909
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 1 cut(s) 1055
Lsp1109I GCAGC 3 cut(s) 379, 421, 424
LweI GCATC 4 cut(s) 69, 471, 1163, 1360
MaeI CTAG 3 cut(s) 102, 590, 1178
MaeII ACGT 1 cut(s) 1122
MalI GATC 4 cut(s) 66, 282, 444, 911
MboI GATC 4 cut(s) 64, 280, 442, 909
MfeI CAATTG 1 cut(s) 555
MflI RGATCY 2 cut(s) 64, 442
MhlI GDGCHC 1 cut(s) 589
MlyI GAGTC 2 cut(s) 964, 1211
MmeI TCCRAC 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 3 cut(s) 45, 363, 704
MspA1I CMGCKG 2 cut(s) 392, 412
MspI CCGG 1 cut(s) 630
MspR9I CCNGG 2 cut(s) 882, 1313
MunI CAATTG 1 cut(s) 555
MvaI CCWGG 2 cut(s) 882, 1313
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 1340
NdeI CATATG 1 cut(s) 799
NdeII GATC 4 cut(s) 64, 280, 442, 909
NlaIII CATG 4 cut(s) 197, 224, 235, 428
NlaIV GGNNCC 1 cut(s) 1057
PaeR7I CTCGAG 1 cut(s) 1384
PceI AGGCCT 1 cut(s) 1161
PciSI GCTCTTC 1 cut(s) 1041
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 5 cut(s) 138, 152, 269, 370, 1081
PflMI CCANNNNNTGG 1 cut(s) 803
PkrI GCNGC 3 cut(s) 394, 411, 439
PleI GAGTC 2 cut(s) 964, 1210
PpsI GAGTC 2 cut(s) 964, 1210
Psp124BI GAGCTC 1 cut(s) 589
Psp6I CCWGG 2 cut(s) 880, 1311
PspGI CCWGG 2 cut(s) 880, 1311
PspN4I GGNNCC 1 cut(s) 1057
PspXI VCTCGAGB 1 cut(s) 1384
PstI CTGCAG 3 cut(s) 397, 442, 794
PsuI RGATCY 2 cut(s) 64, 442
PvuII CAGCTG 2 cut(s) 392, 412
RsaI GTAC 4 cut(s) 530, 649, 816, 1248
RsaNI GTAC 4 cut(s) 529, 648, 815, 1247
SacI GAGCTC 1 cut(s) 589
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 3 cut(s) 45, 363, 704
SatI GCNGC 3 cut(s) 393, 410, 438
Sau3AI GATC 4 cut(s) 64, 280, 442, 909
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 2 cut(s) 964, 1211
ScrFI CCNGG 2 cut(s) 882, 1313
SduI GDGCHC 1 cut(s) 589
SfaNI GCATC 4 cut(s) 69, 471, 1163, 1360
SfcI CTRYAG 3 cut(s) 393, 438, 790
Sfr274I CTCGAG 1 cut(s) 1384
SlaI CTCGAG 1 cut(s) 1384
SmlI CTYRAG 2 cut(s) 1304, 1384
SmoI CTYRAG 2 cut(s) 1304, 1384
SseBI AGGCCT 1 cut(s) 1161
SspMI CTAG 3 cut(s) 102, 590, 1178
SstI GAGCTC 1 cut(s) 589
StuI AGGCCT 1 cut(s) 1161
StyD4I CCNGG 2 cut(s) 880, 1311
StyI CCWWGG 3 cut(s) 456, 854, 1105
TaiI ACGT 1 cut(s) 1125
TaqI TCGA 2 cut(s) 1205, 1385
TatI WGTACW 3 cut(s) 528, 814, 1246
TfiI GAWTC 5 cut(s) 138, 152, 269, 370, 1081
Tru1I TTAA 3 cut(s) 45, 363, 704
Tru9I TTAA 3 cut(s) 45, 363, 704
TscAI CASTG 3 cut(s) 383, 955, 1155
TseI GCWGC 3 cut(s) 392, 409, 437
TspDTI ATGAA 3 cut(s) 135, 248, 1314
TspGWI ACGGA 2 cut(s) 147, 847
TspRI CASTG 3 cut(s) 383, 955, 1155
Van91I CCANNNNNTGG 1 cut(s) 803
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 5 cut(s) 625, 700, 928, 1132, 1271
XbaI TCTAGA 2 cut(s) 589, 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 1384
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 3 cut(s) 102, 590, 1178
ZrmI AGTACT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.