Rh1BG402200

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0014214
Physical Location & Seq
Forward (+)
52606350 .. 52609308
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG402200.1

Sequence Viewer

Length: 1320 bp
ATGTACATGCATCCGGGTTTTTATTTGTATCATTTGGTCATCCTTTTGCAGCTTCCAATTAAGTCATTACCTATTCAAGATCCATCAGCACCGATGCAAGTGGATAAAAAAGAAACTAGCATAGTCTTGAAGGATGAAACGAAAAACGGAGGATTCGCGCAAGCAGAATCTATCATCTTCTACACTAAGAGACTTCAAGATGATTTGCATGAGATGGGGTTGAAAGTAAAACAGCATGAGGAGGACATGAAACTTCTGAGGTCTCAGAAAAACAAATTAGAAGAATCTATTCTTGATCTGCAAGTTATGTTAGGCAACTATCATTCCTCAACCACAACTACTACTGCAAATGATAACCATAGTCAGGAGGAAACAATTAAAAAGATTCTTCAGTGCGAGAAGTCAGCTGCAGGCATTTTATGGCAGCTGAAAGCTCGTCATGGTAATCAGGCTGCAGATCTTCCCCTGACCAAGGATATTGTGGGTATTGTTGCGATGCTTGGGAAAGTAGATGATGATAATCTTAGCAGGCTTTTGTCAGAGTACTTGGGACTGGAGACTATGTTAGCAATTGTCTGCAAGACTTTTGATGGTGTTAGAGCTCTAGAATTGTATGACAACGAAGGTTGCATAAACCTGAATTCCGGTCTTCACGGGCTTGGTACTTCTATTGGGAGGAAATTGGAAGGTCGGTTTCTGGTCATTTGTCTTGAAAATTTAAGACCATATGCTGGTGAGTTTGTACATAATGACCCCCAACGGAGGCTTGATATTCTAAAACCAAGGTTGCTGAATGGGGAGTGTCCACCTGGATTTCTTGGCTATGCTGTAAATATGATCAATGTGGATAGCACAAATTTATTTTGTCTCACAGCCAGTGGACACGGCCTGAGAGAGACTCTACTTTATAGCCTCTTTTCTCGCTTGCAAGTGTATAAAACAAGAGCAGATATGGTATCTGCTCTTCCTTGTATAACTGATGGAGCCATTTCTTTAGATGGTGGGATGATTCAGAGGACTGGTGTGTTTTCCCTTGGCAACAGGGAAGACGTAGGTGTGAATTTTCCAAAGCTCTCAGTGGCATCAGGCCTGCCCGAAAGCTATCTAGAAACAGAAAGACAAATCAATGAGTCGAAATGGAGAAAAGAAAAATTGATTGAAGATACGAAGAGAGTACAGGCATTATGGGACAATGCAAAATTCAATTTTGACAGAAAGAAAAAAGAGTTCCTCAAGTTCCTGGCTGATAGTTCATCTTATGCAACTCAGGCAACTCAGCATCAAATTATGGGGGCGCAGAATAGATTTACCTCGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

439

Amino Acids

49.34

Weight (kDa)

6.61

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 731
AccII CGCG 1 cut(s) 158
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 5 cut(s) 640, 715, 856, 1060, 1199
AcuI CTGAAG 1 cut(s) 374
AfaI GTAC 5 cut(s) 5, 545, 664, 744, 1176
AfiI CCNNNNNNNGG 4 cut(s) 364, 472, 731, 762
AgsI TTSAA 7 cut(s) 77, 130, 197, 223, 713, 1160, 1204
AjnI CCWGG 2 cut(s) 808, 1239
AluBI AGCT 8 cut(s) 52, 407, 427, 434, 602, 1072, 1101, 1317
AluI AGCT 8 cut(s) 52, 407, 427, 434, 602, 1072, 1101, 1317
Alw21I GWGCWC 1 cut(s) 604
Alw26I GTCTC 5 cut(s) 184, 267, 551, 872, 890
AlwI GGATC 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 1312
AoxI GGCC 2 cut(s) 886, 1087
ApeKI GCWGC 4 cut(s) 49, 407, 424, 452
ApoI RAATTY 5 cut(s) 640, 715, 856, 1060, 1199
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 2 cut(s) 160, 1297
AsuC2I CCSGG 1 cut(s) 15
AsuHPI GGTGA 1 cut(s) 746
AvaI CYCGRG 1 cut(s) 1312
BanII GRGCYC 1 cut(s) 604
BarI GAAGNNNNNNTAC 2 cut(s) 948, 980
BbsI GAAGAC 2 cut(s) 641, 1053
Bbv12I GWGCWC 1 cut(s) 604
BbvI GCAGC 4 cut(s) 61, 394, 436, 439
BccI CCATC 5 cut(s) 91, 208, 584, 974, 992
BceAI ACGGC 1 cut(s) 901
BciT130I CCWGG 2 cut(s) 810, 1241
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 5 cut(s) 184, 267, 551, 872, 890
BfaI CTAG 3 cut(s) 117, 605, 1106
BfmI CTRYAG 2 cut(s) 408, 453
BglII AGATCT 1 cut(s) 457
BisI GCNGC 4 cut(s) 50, 408, 425, 453
BlsI GCNGC 4 cut(s) 51, 409, 426, 454
BmcAI AGTACT 1 cut(s) 545
Bme1390I CCNGG 3 cut(s) 15, 810, 1241
BmeT110I CYCGRG 1 cut(s) 1312
BmiI GGNNCC 1 cut(s) 985
BmrFI CCNGG 3 cut(s) 15, 810, 1241
BmsI GCATC 5 cut(s) 19, 84, 486, 1091, 1288
BpiI GAAGAC 2 cut(s) 641, 1053
BplI GAGNNNNNCTC 2 cut(s) 883, 915
BpmI CTGGAG 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 1217
BpuMI CCSGG 1 cut(s) 15
BsaBI GATNNNNATC 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 471, 782, 1033
BsaWI WCCGGW 1 cut(s) 644
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 4 cut(s) 364, 472, 731, 762
Bse1I ACTGG 3 cut(s) 558, 876, 1024
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 2 cut(s) 810, 1241
BseDI CCNNGG 3 cut(s) 471, 782, 1033
BseGI GGATG 4 cut(s) 10, 39, 139, 1011
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 4 cut(s) 364, 472, 731, 762
BseMII CTCAG 6 cut(s) 248, 278, 881, 1089, 1280, 1289
BseNI ACTGG 3 cut(s) 558, 876, 1024
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 4 cut(s) 61, 394, 436, 439
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 888, 1089
BsiHKAI GWGCWC 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 1312
BsiSI CCGG 2 cut(s) 14, 645
BslFI GGGAC 2 cut(s) 564, 1202
BslI CCNNNNNNNGG 4 cut(s) 364, 472, 731, 762
BsmAI GTCTC 5 cut(s) 184, 267, 551, 872, 890
BsmFI GGGAC 2 cut(s) 564, 1202
BsnI GGCC 2 cut(s) 888, 1089
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 1312
Bsp1286I GDGCHC 1 cut(s) 604
Bsp1407I TGTACA 2 cut(s) 3, 742
Bsp143I GATC 4 cut(s) 79, 295, 457, 837
BspANI GGCC 2 cut(s) 888, 1089
BspCNI CTCAG 6 cut(s) 249, 277, 882, 1088, 1279, 1288
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 985
BspMAI CTGCAG 2 cut(s) 412, 457
BspPI GGATC 1 cut(s) 74
BspQI GCTCTTC 1 cut(s) 969
BspTNI GGTCTC 1 cut(s) 267
BsrGI TGTACA 2 cut(s) 3, 742
BsrI ACTGG 3 cut(s) 558, 876, 1024
BssECI CCNNGG 3 cut(s) 471, 782, 1033
BssMI GATC 4 cut(s) 79, 295, 457, 837
BssT1I CCWWGG 3 cut(s) 471, 782, 1033
Bst2UI CCWGG 2 cut(s) 810, 1241
Bst6I CTCTTC 2 cut(s) 969, 1163
BstAUI TGTACA 2 cut(s) 3, 742
BstC8I GCNNGC 5 cut(s) 162, 412, 530, 926, 1091
BstDEI CTNAG 8 cut(s) 186, 257, 264, 524, 890, 1075, 1266, 1275
BstENI CCTNNNNNAGG 1 cut(s) 470
BstF5I GGATG 4 cut(s) 10, 39, 139, 1011
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 2 cut(s) 160, 1297
BstKTI GATC 4 cut(s) 82, 298, 460, 840
BstMAI GTCTC 5 cut(s) 184, 267, 551, 872, 890
BstMBI GATC 4 cut(s) 79, 295, 457, 837
BstMWI GCNNNNNNNGC 1 cut(s) 1268
BstNI CCWGG 2 cut(s) 810, 1241
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 3 cut(s) 13, 808, 1239
BstSFI CTRYAG 2 cut(s) 408, 453
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 4 cut(s) 61, 394, 436, 439
BstV2I GAAGAC 2 cut(s) 641, 1053
BstX2I RGATCY 2 cut(s) 79, 457
BstYI RGATCY 2 cut(s) 79, 457
BsuRI GGCC 2 cut(s) 888, 1089
BtgZI GCGATG 1 cut(s) 509
BtsCI GGATG 4 cut(s) 10, 39, 139, 1011
BtsIMutI CAGTG 3 cut(s) 398, 883, 1083
Cac8I GCNNGC 5 cut(s) 162, 412, 530, 926, 1091
CfoI GCGC 2 cut(s) 160, 1297
Csp6I GTAC 5 cut(s) 4, 544, 663, 743, 1175
CviAII CATG 5 cut(s) 7, 209, 236, 247, 440
CviQI GTAC 5 cut(s) 4, 544, 663, 743, 1175
DdeI CTNAG 8 cut(s) 186, 257, 264, 524, 890, 1075, 1266, 1275
DpnI GATC 4 cut(s) 81, 297, 459, 839
DpnII GATC 4 cut(s) 79, 295, 457, 837
Eam1104I CTCTTC 2 cut(s) 969, 1163
EarI CTCTTC 2 cut(s) 969, 1163
Ecl136II GAGCTC 1 cut(s) 602
Eco130I CCWWGG 3 cut(s) 471, 782, 1033
Eco147I AGGCCT 1 cut(s) 1089
Eco24I GRGCYC 1 cut(s) 604
Eco31I GGTCTC 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 1312
EcoICRI GAGCTC 1 cut(s) 602
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 640
EcoRII CCWGG 2 cut(s) 808, 1239
EcoT14I CCWWGG 3 cut(s) 471, 782, 1033
EcoT22I ATGCAT 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 604
ErhI CCWWGG 3 cut(s) 471, 782, 1033
FaeI CATG 5 cut(s) 10, 212, 239, 250, 443
FaqI GGGAC 2 cut(s) 564, 1202
FatI CATG 5 cut(s) 6, 208, 235, 246, 439
FauNDI CATATG 1 cut(s) 727
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 4 cut(s) 50, 408, 425, 453
FokI GGATG 3 cut(s) 26, 146, 1018
FriOI GRGCYC 1 cut(s) 604
Fsp4HI GCNGC 4 cut(s) 50, 408, 425, 453
FspBI CTAG 3 cut(s) 117, 605, 1106
GlaI GCGC 2 cut(s) 159, 1296
GluI GCNGC 4 cut(s) 50, 408, 425, 453
GsuI CTGGAG 1 cut(s) 575
HaeIII GGCC 2 cut(s) 888, 1089
HapII CCGG 2 cut(s) 14, 645
HhaI GCGC 2 cut(s) 160, 1297
Hin1II CATG 5 cut(s) 10, 212, 239, 250, 443
Hin6I GCGC 2 cut(s) 158, 1295
HinP1I GCGC 2 cut(s) 158, 1295
HinfI GANTC 7 cut(s) 153, 167, 284, 385, 898, 1009, 1130
HpaII CCGG 2 cut(s) 14, 645
HphI GGTGA 1 cut(s) 746
Hpy166II GTNNAC 2 cut(s) 806, 881
Hpy188I TCNGA 4 cut(s) 258, 267, 541, 1014
Hpy188III TCNNGA 8 cut(s) 77, 127, 197, 293, 365, 605, 710, 1106
Hpy8I GTNNAC 2 cut(s) 806, 881
HpyAV CCTTC 3 cut(s) 124, 617, 680
HpyCH4IV ACGT 1 cut(s) 1050
HpyF10VI GCNNNNNNNGC 1 cut(s) 1268
HpyF3I CTNAG 8 cut(s) 186, 257, 264, 524, 890, 1075, 1266, 1275
HpySE526I ACGT 1 cut(s) 1050
Hsp92II CATG 5 cut(s) 10, 212, 239, 250, 443
HspAI GCGC 2 cut(s) 158, 1295
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 4 cut(s) 79, 295, 457, 837
LguI GCTCTTC 1 cut(s) 969
LmnI GCTCC 1 cut(s) 983
Lsp1109I GCAGC 4 cut(s) 61, 394, 436, 439
LweI GCATC 5 cut(s) 19, 84, 486, 1091, 1288
MaeI CTAG 3 cut(s) 117, 605, 1106
MaeII ACGT 1 cut(s) 1050
MalI GATC 4 cut(s) 81, 297, 459, 839
MboI GATC 4 cut(s) 79, 295, 457, 837
MboII GAAGA 9 cut(s) 169, 293, 380, 452, 641, 956, 1058, 1172, 1180
MfeI CAATTG 1 cut(s) 570
MflI RGATCY 2 cut(s) 79, 457
MhlI GDGCHC 1 cut(s) 604
MlyI GAGTC 2 cut(s) 892, 1139
Mph1103I ATGCAT 1 cut(s) 12
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 3 cut(s) 60, 378, 719
MspA1I CMGCKG 2 cut(s) 407, 427
MspI CCGG 2 cut(s) 14, 645
MspR9I CCNGG 3 cut(s) 15, 810, 1241
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 2 cut(s) 810, 1241
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 1268
NciI CCSGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 727
NdeII GATC 4 cut(s) 79, 295, 457, 837
NlaIII CATG 5 cut(s) 10, 212, 239, 250, 443
NlaIV GGNNCC 1 cut(s) 985
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 1312
PceI AGGCCT 1 cut(s) 1089
PciSI GCTCTTC 1 cut(s) 969
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 5 cut(s) 153, 167, 284, 385, 1009
PflMI CCANNNNNTGG 1 cut(s) 731
PkrI GCNGC 4 cut(s) 51, 409, 426, 454
PleI GAGTC 2 cut(s) 892, 1138
PpsI GAGTC 2 cut(s) 892, 1138
Psp124BI GAGCTC 1 cut(s) 604
Psp6I CCWGG 2 cut(s) 808, 1239
PspGI CCWGG 2 cut(s) 808, 1239
PspN4I GGNNCC 1 cut(s) 985
PspXI VCTCGAGB 1 cut(s) 1312
PstI CTGCAG 2 cut(s) 412, 457
PsuI RGATCY 2 cut(s) 79, 457
PvuII CAGCTG 2 cut(s) 407, 427
RsaI GTAC 5 cut(s) 5, 545, 664, 744, 1176
RsaNI GTAC 5 cut(s) 4, 544, 663, 743, 1175
SacI GAGCTC 1 cut(s) 604
SapI GCTCTTC 1 cut(s) 969
SaqAI TTAA 3 cut(s) 60, 378, 719
SatI GCNGC 4 cut(s) 50, 408, 425, 453
Sau3AI GATC 4 cut(s) 79, 295, 457, 837
ScaI AGTACT 1 cut(s) 545
SchI GAGTC 2 cut(s) 892, 1139
ScrFI CCNGG 3 cut(s) 15, 810, 1241
SduI GDGCHC 1 cut(s) 604
SfaNI GCATC 5 cut(s) 19, 84, 486, 1091, 1288
SfcI CTRYAG 2 cut(s) 408, 453
Sfr274I CTCGAG 1 cut(s) 1312
SlaI CTCGAG 1 cut(s) 1312
SmlI CTYRAG 2 cut(s) 1232, 1312
SmoI CTYRAG 2 cut(s) 1232, 1312
SseBI AGGCCT 1 cut(s) 1089
SspMI CTAG 3 cut(s) 117, 605, 1106
SstI GAGCTC 1 cut(s) 604
StuI AGGCCT 1 cut(s) 1089
StyD4I CCNGG 3 cut(s) 13, 808, 1239
StyI CCWWGG 3 cut(s) 471, 782, 1033
TaiI ACGT 1 cut(s) 1053
TaqI TCGA 2 cut(s) 1133, 1313
TatI WGTACW 4 cut(s) 3, 543, 742, 1174
TfiI GAWTC 5 cut(s) 153, 167, 284, 385, 1009
Tru1I TTAA 3 cut(s) 60, 378, 719
Tru9I TTAA 3 cut(s) 60, 378, 719
TscAI CASTG 3 cut(s) 398, 883, 1083
TseI GCWGC 4 cut(s) 49, 407, 424, 452
TspDTI ATGAA 3 cut(s) 150, 263, 1242
TspGWI ACGGA 2 cut(s) 162, 775
TspRI CASTG 3 cut(s) 398, 883, 1083
Van91I CCANNNNNTGG 1 cut(s) 731
XagI CCTNNNNNAGG 1 cut(s) 470
XapI RAATTY 5 cut(s) 640, 715, 856, 1060, 1199
XbaI TCTAGA 2 cut(s) 604, 1105
XceI RCATGY 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XhoI CTCGAG 1 cut(s) 1312
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 3 cut(s) 117, 605, 1106
ZrmI AGTACT 1 cut(s) 545
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.