Rh1AG446900

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014214
Physical Location & Seq
Forward (+)
66699906 .. 66703747
3842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG446900.1

Sequence Viewer

Length: 1296 bp
ATGTTCCAACCCAACAACAACCACCAGCTTCCAATTAAGTCATTACCTACTCAAGATCCATCAGCACTGATGCAAGTGGATAAAAAAGAAACTAGCATAGTCTTGAAGGATGAAACGCAAAACGGAGGATTCGCGCAAGCAGAATCTATCATCTTCTACACTAAGAGACTTCAAGATGATTTGCATGATATGGGGTTGAAAGTAAAACTGCATGAGGAGAACATGAAACTTCTGAGGTCTCAGAAAAACAAATTAGAAGAATCTATTCTTGATCTGCAAGTTATGTTAGGCAACTATCATTCCACAACCACAACTACTACTGCAAATGATAACCACAGTCAGGAGGAAACAATTAAAAAGATTCTTCAGTGCGAGAAGTCAGCTGCAGGCATTTTATGGCAGCTGAAAGCTCGTCATGGTAATCAGGATGCGGATCTTCCCCTGACCAAGGATATTGTGGGTATTGTTGCGATGCTTGGGAAAGTAGATGATGATAATCTTAGCAGGCTTTTGTCAGAGTACTTGGGACTGGAGACTATGTTAGCAATTGTCTGCAAGACTTATGATGGTGTCAGAGCTCTAGAATTGTATGACAATGAAGGTTGCATAAACCTGAATTCCGGTCTTCACGGGCTTGGTACTGCTATTGGGAGGAAATTGGAAGGTCGGTTTCTGGTCATTTGTCTTGAAAATTTAAGACCATATGCTGGTGAGTTTGTACATAATGACCCCCAACGGAGGCTTGATATTCTAAAACCAAGGTTGCCGAATGGGGAGTGTCCACCTGGATTTCTTGGCTATGCTGTAAATATGATCAATGTGGATAGCACAAATTTATTTTGTCTCACAGCCAGTGGACACGGCCTGAGAGAGACTCTACTTTATAGCCTCTTTTCTCGCTTGCAAGTGTATAAAACAAGAGCAGATATGGTATCTGCTCTTCCTTGTATAACTGATGGAGCCATTTCTTTAGATGGTGGGATGATTCAGAGGACTGGTGTGTTTTCCCTTGGCAACAGGGAAGACGTAGGTGTGAAGTTTCCAAAGCTCTCAGTGGCATCAGGCCTGCCCGAAAGCTATCTAGAAACAGAAAGACAAATCAATGAGTCGAAATGGAGAAAAGAAAAATTGATTGAAGATACGAAGAGAGAACAGGCATTATGGGACAATGCAAAATTCAATTTTGACAGAAAGAAAAAAGAGTTCCTCAAGTTCCTGGCTGATAGTTCATCTTATGCAACTCAGGCAACTCAGCATCAAATTATGGGGGCGCAGAATAGATTTACCTCGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

431

Amino Acids

48.39

Weight (kDa)

6.39

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AccII CGCG 1 cut(s) 134
AciI CCGC 1 cut(s) 431
AclWI GGATC 2 cut(s) 50, 441
AcsI RAATTY 4 cut(s) 616, 691, 832, 1175
AcuI CTGAAG 1 cut(s) 350
AfaI GTAC 3 cut(s) 521, 640, 720
AfiI CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
AgsI TTSAA 6 cut(s) 106, 173, 199, 689, 1136, 1180
AjnI CCWGG 2 cut(s) 784, 1215
AluBI AGCT 8 cut(s) 28, 383, 403, 410, 578, 1048, 1077, 1293
AluI AGCT 8 cut(s) 28, 383, 403, 410, 578, 1048, 1077, 1293
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 5 cut(s) 160, 243, 527, 848, 866
AlwI GGATC 2 cut(s) 50, 441
Ama87I CYCGRG 1 cut(s) 1288
AoxI GGCC 2 cut(s) 862, 1063
ApeKI GCWGC 2 cut(s) 383, 400
ApoI RAATTY 4 cut(s) 616, 691, 832, 1175
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 136, 1273
AsuHPI GGTGA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 1288
BanII GRGCYC 1 cut(s) 580
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BbsI GAAGAC 2 cut(s) 617, 1029
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 2 cut(s) 370, 412
BccI CCATC 4 cut(s) 67, 560, 950, 968
BceAI ACGGC 1 cut(s) 877
BciT130I CCWGG 2 cut(s) 786, 1217
BclI TGATCA 1 cut(s) 813
BcoDI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BfaI CTAG 3 cut(s) 93, 581, 1082
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 2 cut(s) 384, 401
BlsI GCNGC 2 cut(s) 385, 402
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 2 cut(s) 786, 1217
BmeT110I CYCGRG 1 cut(s) 1288
BmiI GGNNCC 1 cut(s) 961
BmrFI CCNGG 2 cut(s) 786, 1217
BmsI GCATC 5 cut(s) 60, 418, 462, 1067, 1264
BpiI GAAGAC 2 cut(s) 617, 1029
BplI GAGNNNNNCTC 2 cut(s) 859, 891
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 36, 1193
BsaBI GATNNNNATC 2 cut(s) 432, 495
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 3 cut(s) 447, 758, 1009
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
Bse1I ACTGG 3 cut(s) 534, 852, 1000
Bse8I GATNNNNATC 2 cut(s) 432, 495
BseBI CCWGG 2 cut(s) 786, 1217
BseDI CCNNGG 3 cut(s) 447, 758, 1009
BseGI GGATG 3 cut(s) 115, 433, 987
BseJI GATNNNNATC 2 cut(s) 432, 495
BseLI CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
BseMII CTCAG 6 cut(s) 224, 254, 857, 1065, 1256, 1265
BseNI ACTGG 3 cut(s) 534, 852, 1000
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 2 cut(s) 370, 412
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 2 cut(s) 864, 1065
BsiHKAI GWGCWC 1 cut(s) 580
BsiHKCI CYCGRG 1 cut(s) 1288
BsiSI CCGG 1 cut(s) 621
BslFI GGGAC 2 cut(s) 540, 1178
BslI CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
BsmAI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BsmFI GGGAC 2 cut(s) 540, 1178
BsnI GGCC 2 cut(s) 864, 1065
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 1288
Bsp1286I GDGCHC 1 cut(s) 580
Bsp1407I TGTACA 1 cut(s) 718
Bsp143I GATC 4 cut(s) 55, 271, 433, 813
BspACI CCGC 1 cut(s) 431
BspANI GGCC 2 cut(s) 864, 1065
BspCNI CTCAG 6 cut(s) 225, 253, 858, 1064, 1255, 1264
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 1 cut(s) 961
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 2 cut(s) 50, 441
BspQI GCTCTTC 1 cut(s) 945
BspTNI GGTCTC 1 cut(s) 243
BsrGI TGTACA 1 cut(s) 718
BsrI ACTGG 3 cut(s) 534, 852, 1000
BssECI CCNNGG 3 cut(s) 447, 758, 1009
BssMI GATC 4 cut(s) 55, 271, 433, 813
BssT1I CCWWGG 3 cut(s) 447, 758, 1009
Bst2UI CCWGG 2 cut(s) 786, 1217
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 2 cut(s) 945, 1139
BstAUI TGTACA 1 cut(s) 718
BstC8I GCNNGC 5 cut(s) 138, 388, 506, 902, 1067
BstDEI CTNAG 8 cut(s) 162, 233, 240, 500, 866, 1051, 1242, 1251
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 3 cut(s) 115, 433, 987
BstFNI CGCG 1 cut(s) 134
BstHHI GCGC 2 cut(s) 136, 1273
BstKTI GATC 4 cut(s) 58, 274, 436, 816
BstMAI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BstMBI GATC 4 cut(s) 55, 271, 433, 813
BstMWI GCNNNNNNNGC 1 cut(s) 1244
BstNI CCWGG 2 cut(s) 786, 1217
BstSCI CCNGG 2 cut(s) 784, 1215
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 2 cut(s) 370, 412
BstV2I GAAGAC 2 cut(s) 617, 1029
BstX2I RGATCY 2 cut(s) 55, 433
BstYI RGATCY 2 cut(s) 55, 433
BsuRI GGCC 2 cut(s) 864, 1065
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 3 cut(s) 115, 433, 987
BtsIMutI CAGTG 4 cut(s) 65, 374, 859, 1059
Cac8I GCNNGC 5 cut(s) 138, 388, 506, 902, 1067
CfoI GCGC 2 cut(s) 136, 1273
Csp6I GTAC 3 cut(s) 520, 639, 719
CviAII CATG 4 cut(s) 185, 212, 223, 416
CviQI GTAC 3 cut(s) 520, 639, 719
DdeI CTNAG 8 cut(s) 162, 233, 240, 500, 866, 1051, 1242, 1251
DpnI GATC 4 cut(s) 57, 273, 435, 815
DpnII GATC 4 cut(s) 55, 271, 433, 813
Eam1104I CTCTTC 2 cut(s) 945, 1139
EarI CTCTTC 2 cut(s) 945, 1139
Ecl136II GAGCTC 1 cut(s) 578
Eco130I CCWWGG 3 cut(s) 447, 758, 1009
Eco147I AGGCCT 1 cut(s) 1065
Eco24I GRGCYC 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 578
Eco57I CTGAAG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 1288
EcoICRI GAGCTC 1 cut(s) 578
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 616
EcoRII CCWGG 2 cut(s) 784, 1215
EcoT14I CCWWGG 3 cut(s) 447, 758, 1009
EcoT38I GRGCYC 1 cut(s) 580
ErhI CCWWGG 3 cut(s) 447, 758, 1009
FaeI CATG 4 cut(s) 188, 215, 226, 419
FaqI GGGAC 2 cut(s) 540, 1178
FatI CATG 4 cut(s) 184, 211, 222, 415
FauNDI CATATG 1 cut(s) 703
FbaI TGATCA 1 cut(s) 813
Fnu4HI GCNGC 2 cut(s) 384, 401
FokI GGATG 3 cut(s) 122, 440, 994
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 2 cut(s) 384, 401
FspBI CTAG 3 cut(s) 93, 581, 1082
GlaI GCGC 2 cut(s) 135, 1272
GluI GCNGC 2 cut(s) 384, 401
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 2 cut(s) 864, 1065
HapII CCGG 1 cut(s) 621
HhaI GCGC 2 cut(s) 136, 1273
Hin1II CATG 4 cut(s) 188, 215, 226, 419
Hin6I GCGC 2 cut(s) 134, 1271
HinP1I GCGC 2 cut(s) 134, 1271
HinfI GANTC 7 cut(s) 129, 143, 260, 361, 874, 985, 1106
HpaII CCGG 1 cut(s) 621
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 2 cut(s) 782, 857
Hpy188I TCNGA 5 cut(s) 234, 243, 517, 575, 990
Hpy188III TCNNGA 9 cut(s) 53, 103, 173, 269, 341, 425, 581, 686, 1082
Hpy8I GTNNAC 2 cut(s) 782, 857
HpyAV CCTTC 3 cut(s) 100, 593, 656
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 1 cut(s) 1026
HpyF10VI GCNNNNNNNGC 1 cut(s) 1244
HpyF3I CTNAG 8 cut(s) 162, 233, 240, 500, 866, 1051, 1242, 1251
HpySE526I ACGT 1 cut(s) 1026
Hsp92II CATG 4 cut(s) 188, 215, 226, 419
HspAI GCGC 2 cut(s) 134, 1271
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 4 cut(s) 55, 271, 433, 813
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 1 cut(s) 959
Lsp1109I GCAGC 2 cut(s) 370, 412
LweI GCATC 5 cut(s) 60, 418, 462, 1067, 1264
MaeI CTAG 3 cut(s) 93, 581, 1082
MaeII ACGT 1 cut(s) 1026
MalI GATC 4 cut(s) 57, 273, 435, 815
MboI GATC 4 cut(s) 55, 271, 433, 813
MboII GAAGA 9 cut(s) 145, 269, 356, 428, 617, 932, 1034, 1148, 1156
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 2 cut(s) 55, 433
MhlI GDGCHC 1 cut(s) 580
MlyI GAGTC 2 cut(s) 868, 1115
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 9 cut(s) 119, 208, 228, 337, 645, 732, 899, 984, 1217
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 3 cut(s) 36, 354, 695
MspA1I CMGCKG 2 cut(s) 383, 403
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 2 cut(s) 786, 1217
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 2 cut(s) 786, 1217
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 1244
NdeI CATATG 1 cut(s) 703
NdeII GATC 4 cut(s) 55, 271, 433, 813
NlaIII CATG 4 cut(s) 188, 215, 226, 419
NlaIV GGNNCC 1 cut(s) 961
PaeR7I CTCGAG 1 cut(s) 1288
PceI AGGCCT 1 cut(s) 1065
PciSI GCTCTTC 1 cut(s) 945
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 5 cut(s) 129, 143, 260, 361, 985
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 2 cut(s) 385, 402
PleI GAGTC 2 cut(s) 868, 1114
PpsI GAGTC 2 cut(s) 868, 1114
Psp124BI GAGCTC 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 784, 1215
PspGI CCWGG 2 cut(s) 784, 1215
PspN4I GGNNCC 1 cut(s) 961
PspXI VCTCGAGB 1 cut(s) 1288
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 2 cut(s) 55, 433
PvuII CAGCTG 2 cut(s) 383, 403
RsaI GTAC 3 cut(s) 521, 640, 720
RsaNI GTAC 3 cut(s) 520, 639, 719
SacI GAGCTC 1 cut(s) 580
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 3 cut(s) 36, 354, 695
SatI GCNGC 2 cut(s) 384, 401
Sau3AI GATC 4 cut(s) 55, 271, 433, 813
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 2 cut(s) 868, 1115
ScrFI CCNGG 2 cut(s) 786, 1217
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 5 cut(s) 60, 418, 462, 1067, 1264
SfcI CTRYAG 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 1288
SlaI CTCGAG 1 cut(s) 1288
SmlI CTYRAG 3 cut(s) 51, 1208, 1288
SmoI CTYRAG 3 cut(s) 51, 1208, 1288
SseBI AGGCCT 1 cut(s) 1065
SsiI CCGC 1 cut(s) 431
SspMI CTAG 3 cut(s) 93, 581, 1082
SstI GAGCTC 1 cut(s) 580
StuI AGGCCT 1 cut(s) 1065
StyD4I CCNGG 2 cut(s) 784, 1215
StyI CCWWGG 3 cut(s) 447, 758, 1009
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 1 cut(s) 1029
TaqI TCGA 2 cut(s) 1109, 1289
TatI WGTACW 2 cut(s) 519, 718
TfiI GAWTC 5 cut(s) 129, 143, 260, 361, 985
Tru1I TTAA 3 cut(s) 36, 354, 695
Tru9I TTAA 3 cut(s) 36, 354, 695
TscAI CASTG 4 cut(s) 72, 374, 859, 1059
TseI GCWGC 2 cut(s) 383, 400
TspDTI ATGAA 4 cut(s) 126, 239, 612, 1218
TspGWI ACGGA 2 cut(s) 138, 751
TspRI CASTG 4 cut(s) 72, 374, 859, 1059
Van91I CCANNNNNTGG 1 cut(s) 707
XagI CCTNNNNNAGG 1 cut(s) 446
XapI RAATTY 4 cut(s) 616, 691, 832, 1175
XbaI TCTAGA 2 cut(s) 580, 1081
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 1288
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 3 cut(s) 93, 581, 1082
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.