Rroxscaffold_4G00279210

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014214
Physical Location & Seq
Reverse (-)
2054461 .. 2058386
3926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279210.1

Sequence Viewer

Length: 1113 bp
ATGGGGTTGAAAGTAAAACACCATGAGGAGAACATGAAACTTCTGAGGTCTCAGAAAAACAAATTAGAAGAATCTATTCTTGATCTGCAAGTTATGTTAGGCAACTATCATTCCTCAACCACAACTACTACTGCAAATGATAACCATAGTCAGGAGGAAACAATTAAAAAGATTCTTCAGTGCGAGAAGTCAGCTGCAGGCATTTTATGGCAGCTGAAAGCTCGTCATGGTAATCAGGCTGCGGATCTTCCCCTGACCAAGGATATTGTGGGTATTGTTGCGATGCTTGGGAAAGTAGATGATGATAATCTTAGCAGGCTTTTGTCAGAGTACTTGGGACTGGAGACTATGTTAGCAATTGTCTGCAAGACTTATGATGGTGTCAGAGCTCTAGAATTGTATGACAATGAAGGTTGCATAAACCTGAATTCCGGTCTTCACGGGCTTGGTACTTCTATTGGGAGGAAATTGGAAGGTCGGTTTCTGGTCATTTGTCTTGAAAATTTAAGACCATATGCTGGTGAGTTTGTACATAATGACCCCCAACGGAGGCTTGATATTCTAAAACCAAGGTTGCCGAATGGGGAGTGTCCACCTGGATTTCTTGGCTATGCTGTAAATATGATCAATGTGGATAGCACAAATTTATTTTGTCTCACAGCCAGTGGACACGGCCTGAGAGAGACTCTACTTTATAGCCTCTTTTCTCGCTTGCAAGTGTATAAAACAAGAGCAGATATGGTATCTGCTCTTCCTTGTATAACTGATGGAGCCATTTCTTTAGATGGTGGGATGATTCAGAGGACTGGTGTGTTTTCCCTTGGCAACAGGGAAGACGTAGGTGTGAAGTTTCCAAAGCTCTCAGTGGCATCAGGCCTGCCCGAAAGCTATCGATATCGAGAAACAGAGAGACAAATCAATGAGTCGAAATGGAGCAAAGAAAAATTGATTGAAGATACGAAGAGAGAACAGGCATTATGGGACAATGCGAAATTCAATTTTGACAGAAAGAAAAAAGAGTTCCTCAAGTTCCTGGCTGATAGTTCATCTTATGCAACTCAGGCAACTCAGCATCAAATTCTGGGTGCGCAGAATAGATTCACCTCGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

370

Amino Acids

41.48

Weight (kDa)

8.07

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1089
AccB7I CCANNNNNTGG 1 cut(s) 518
AciI CCGC 1 cut(s) 242
AclWI GGATC 1 cut(s) 252
AcsI RAATTY 5 cut(s) 427, 502, 643, 992, 1077
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 3 cut(s) 332, 451, 531
AfiI CCNNNNNNNGG 4 cut(s) 151, 259, 518, 549
AgsI TTSAA 4 cut(s) 10, 500, 953, 997
AjnI CCWGG 2 cut(s) 595, 1032
AluBI AGCT 7 cut(s) 194, 214, 221, 389, 859, 888, 1110
AluI AGCT 7 cut(s) 194, 214, 221, 389, 859, 888, 1110
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 5 cut(s) 54, 338, 659, 677, 904
AlwI GGATC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 1105
AoxI GGCC 2 cut(s) 673, 874
ApeKI GCWGC 3 cut(s) 194, 211, 239
ApoI RAATTY 5 cut(s) 427, 502, 643, 992, 1077
Asp700I GAANNNNTTC 2 cut(s) 75, 1097
AspLEI GCGC 1 cut(s) 1090
AsuHPI GGTGA 2 cut(s) 533, 1093
AvaI CYCGRG 1 cut(s) 1105
BanII GRGCYC 1 cut(s) 391
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
BbsI GAAGAC 2 cut(s) 428, 840
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 181, 223, 226
BccI CCATC 3 cut(s) 371, 761, 779
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 2 cut(s) 597, 1034
BclI TGATCA 1 cut(s) 624
BcoDI GTCTC 5 cut(s) 54, 338, 659, 677, 904
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 3 cut(s) 195, 212, 240
BlsI GCNGC 3 cut(s) 196, 213, 241
BmcAI AGTACT 1 cut(s) 332
Bme1390I CCNGG 2 cut(s) 597, 1034
BmeT110I CYCGRG 1 cut(s) 1105
BmiI GGNNCC 1 cut(s) 772
BmrFI CCNGG 2 cut(s) 597, 1034
BmsI GCATC 3 cut(s) 273, 878, 1081
BpiI GAAGAC 2 cut(s) 428, 840
BplI GAGNNNNNCTC 2 cut(s) 670, 702
BpmI CTGGAG 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 1010
Bsa29I ATCGAT 1 cut(s) 892
BsaBI GATNNNNATC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 258, 569, 820
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 259, 518, 549
Bse1I ACTGG 3 cut(s) 345, 663, 811
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 597, 1034
BseCI ATCGAT 1 cut(s) 892
BseDI CCNNGG 3 cut(s) 258, 569, 820
BseGI GGATG 1 cut(s) 798
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 4 cut(s) 151, 259, 518, 549
BseMII CTCAG 6 cut(s) 35, 65, 668, 876, 1073, 1082
BseNI ACTGG 3 cut(s) 345, 663, 811
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 3 cut(s) 181, 223, 226
BshFI GGCC 2 cut(s) 675, 876
BshVI ATCGAT 1 cut(s) 892
BsiHKAI GWGCWC 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 1105
BsiSI CCGG 1 cut(s) 432
BslFI GGGAC 2 cut(s) 351, 995
BslI CCNNNNNNNGG 4 cut(s) 151, 259, 518, 549
BsmAI GTCTC 5 cut(s) 54, 338, 659, 677, 904
BsmFI GGGAC 2 cut(s) 351, 995
BsnI GGCC 2 cut(s) 675, 876
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 1105
Bsp1286I GDGCHC 1 cut(s) 391
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 3 cut(s) 82, 244, 624
BspACI CCGC 1 cut(s) 242
BspANI GGCC 2 cut(s) 675, 876
BspCNI CTCAG 6 cut(s) 36, 64, 669, 875, 1072, 1081
BspDI ATCGAT 1 cut(s) 892
BspLI GGNNCC 1 cut(s) 772
BspMAI CTGCAG 1 cut(s) 199
BspPI GGATC 1 cut(s) 252
BspQI GCTCTTC 1 cut(s) 756
BspTNI GGTCTC 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 3 cut(s) 345, 663, 811
BssECI CCNNGG 3 cut(s) 258, 569, 820
BssMI GATC 3 cut(s) 82, 244, 624
BssT1I CCWWGG 3 cut(s) 258, 569, 820
Bst2UI CCWGG 2 cut(s) 597, 1034
Bst6I CTCTTC 2 cut(s) 756, 956
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 4 cut(s) 199, 317, 713, 878
BstDEI CTNAG 7 cut(s) 44, 51, 311, 677, 862, 1059, 1068
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 1 cut(s) 798
BstHHI GCGC 1 cut(s) 1090
BstKTI GATC 3 cut(s) 85, 247, 627
BstMAI GTCTC 5 cut(s) 54, 338, 659, 677, 904
BstMBI GATC 3 cut(s) 82, 244, 624
BstMWI GCNNNNNNNGC 1 cut(s) 1061
BstNI CCWGG 2 cut(s) 597, 1034
BstSCI CCNGG 2 cut(s) 595, 1032
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 3 cut(s) 181, 223, 226
BstV2I GAAGAC 2 cut(s) 428, 840
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 892
BsuRI GGCC 2 cut(s) 675, 876
BsuTUI ATCGAT 1 cut(s) 892
BtgZI GCGATG 1 cut(s) 296
BtsCI GGATG 1 cut(s) 798
BtsIMutI CAGTG 3 cut(s) 185, 670, 870
Cac8I GCNNGC 4 cut(s) 199, 317, 713, 878
CfoI GCGC 1 cut(s) 1090
ClaI ATCGAT 1 cut(s) 892
Csp6I GTAC 3 cut(s) 331, 450, 530
CviAII CATG 3 cut(s) 23, 34, 227
CviQI GTAC 3 cut(s) 331, 450, 530
DdeI CTNAG 7 cut(s) 44, 51, 311, 677, 862, 1059, 1068
DpnI GATC 3 cut(s) 84, 246, 626
DpnII GATC 3 cut(s) 82, 244, 624
Eam1104I CTCTTC 2 cut(s) 756, 956
EarI CTCTTC 2 cut(s) 756, 956
Ecl136II GAGCTC 1 cut(s) 389
Eco130I CCWWGG 3 cut(s) 258, 569, 820
Eco147I AGGCCT 1 cut(s) 876
Eco24I GRGCYC 1 cut(s) 391
Eco31I GGTCTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 896
Eco53kI GAGCTC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 1105
EcoICRI GAGCTC 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 595, 1032
EcoRV GATATC 1 cut(s) 896
EcoT14I CCWWGG 3 cut(s) 258, 569, 820
EcoT38I GRGCYC 1 cut(s) 391
ErhI CCWWGG 3 cut(s) 258, 569, 820
FaeI CATG 3 cut(s) 26, 37, 230
FaqI GGGAC 2 cut(s) 351, 995
FatI CATG 3 cut(s) 22, 33, 226
FauNDI CATATG 1 cut(s) 514
FbaI TGATCA 1 cut(s) 624
Fnu4HI GCNGC 3 cut(s) 195, 212, 240
FokI GGATG 1 cut(s) 805
FriOI GRGCYC 1 cut(s) 391
Fsp4HI GCNGC 3 cut(s) 195, 212, 240
FspBI CTAG 1 cut(s) 392
FspI TGCGCA 1 cut(s) 1089
GlaI GCGC 1 cut(s) 1089
GluI GCNGC 3 cut(s) 195, 212, 240
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 2 cut(s) 675, 876
HapII CCGG 1 cut(s) 432
HhaI GCGC 1 cut(s) 1090
Hin1II CATG 3 cut(s) 26, 37, 230
Hin6I GCGC 1 cut(s) 1088
HinP1I GCGC 1 cut(s) 1088
HinfI GANTC 6 cut(s) 71, 172, 685, 796, 923, 1098
HpaII CCGG 1 cut(s) 432
HphI GGTGA 2 cut(s) 533, 1093
Hpy166II GTNNAC 2 cut(s) 593, 668
Hpy188I TCNGA 5 cut(s) 45, 54, 328, 386, 801
Hpy188III TCNNGA 5 cut(s) 80, 152, 392, 497, 899
Hpy8I GTNNAC 2 cut(s) 593, 668
HpyAV CCTTC 2 cut(s) 404, 467
HpyCH4IV ACGT 1 cut(s) 837
HpyCH4V TGCA 7 cut(s) 88, 134, 197, 366, 417, 715, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 1061
HpyF3I CTNAG 7 cut(s) 44, 51, 311, 677, 862, 1059, 1068
HpySE526I ACGT 1 cut(s) 837
Hsp92II CATG 3 cut(s) 26, 37, 230
HspAI GCGC 1 cut(s) 1088
Ksp22I TGATCA 1 cut(s) 624
Kzo9I GATC 3 cut(s) 82, 244, 624
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 2 cut(s) 770, 933
Lsp1109I GCAGC 3 cut(s) 181, 223, 226
LweI GCATC 3 cut(s) 273, 878, 1081
MaeI CTAG 1 cut(s) 392
MaeII ACGT 1 cut(s) 837
MalI GATC 3 cut(s) 84, 246, 626
MboI GATC 3 cut(s) 82, 244, 624
MboII GAAGA 8 cut(s) 80, 167, 239, 428, 743, 845, 965, 973
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 1 cut(s) 391
MlyI GAGTC 2 cut(s) 679, 932
MnlI CCTC 9 cut(s) 19, 39, 124, 148, 456, 543, 710, 795, 1034
MroXI GAANNNNTTC 2 cut(s) 75, 1097
MseI TTAA 2 cut(s) 165, 506
MspA1I CMGCKG 2 cut(s) 194, 214
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 2 cut(s) 597, 1034
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 2 cut(s) 597, 1034
MwoI GCNNNNNNNGC 1 cut(s) 1061
NdeI CATATG 1 cut(s) 514
NdeII GATC 3 cut(s) 82, 244, 624
NlaIII CATG 3 cut(s) 26, 37, 230
NlaIV GGNNCC 1 cut(s) 772
NsbI TGCGCA 1 cut(s) 1089
PaeR7I CTCGAG 1 cut(s) 1105
PceI AGGCCT 1 cut(s) 876
PciSI GCTCTTC 1 cut(s) 756
PdmI GAANNNNTTC 2 cut(s) 75, 1097
PfeI GAWTC 4 cut(s) 71, 172, 796, 1098
PflMI CCANNNNNTGG 1 cut(s) 518
PkrI GCNGC 3 cut(s) 196, 213, 241
PleI GAGTC 2 cut(s) 679, 931
PpsI GAGTC 2 cut(s) 679, 931
Psp124BI GAGCTC 1 cut(s) 391
Psp6I CCWGG 2 cut(s) 595, 1032
PspGI CCWGG 2 cut(s) 595, 1032
PspN4I GGNNCC 1 cut(s) 772
PspXI VCTCGAGB 1 cut(s) 1105
PstI CTGCAG 1 cut(s) 199
PsuI RGATCY 1 cut(s) 244
PvuII CAGCTG 2 cut(s) 194, 214
RsaI GTAC 3 cut(s) 332, 451, 531
RsaNI GTAC 3 cut(s) 331, 450, 530
SacI GAGCTC 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 756
SaqAI TTAA 2 cut(s) 165, 506
SatI GCNGC 3 cut(s) 195, 212, 240
Sau3AI GATC 3 cut(s) 82, 244, 624
ScaI AGTACT 1 cut(s) 332
SchI GAGTC 2 cut(s) 679, 932
ScrFI CCNGG 2 cut(s) 597, 1034
SduI GDGCHC 1 cut(s) 391
SfaNI GCATC 3 cut(s) 273, 878, 1081
SfcI CTRYAG 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 1105
SlaI CTCGAG 1 cut(s) 1105
SmlI CTYRAG 2 cut(s) 1025, 1105
SmoI CTYRAG 2 cut(s) 1025, 1105
SseBI AGGCCT 1 cut(s) 876
SsiI CCGC 1 cut(s) 242
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 391
StuI AGGCCT 1 cut(s) 876
StyD4I CCNGG 2 cut(s) 595, 1032
StyI CCWWGG 3 cut(s) 258, 569, 820
TaiI ACGT 1 cut(s) 840
TaqI TCGA 4 cut(s) 892, 898, 926, 1106
TatI WGTACW 2 cut(s) 330, 529
TfiI GAWTC 4 cut(s) 71, 172, 796, 1098
Tru1I TTAA 2 cut(s) 165, 506
Tru9I TTAA 2 cut(s) 165, 506
TscAI CASTG 3 cut(s) 185, 670, 870
TseI GCWGC 3 cut(s) 194, 211, 239
TspDTI ATGAA 3 cut(s) 50, 423, 1035
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 3 cut(s) 185, 670, 870
Van91I CCANNNNNTGG 1 cut(s) 518
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 5 cut(s) 427, 502, 643, 992, 1077
XbaI TCTAGA 1 cut(s) 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 1105
XmnI GAANNNNTTC 2 cut(s) 75, 1097
XspI CTAG 1 cut(s) 392
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.