RLG00000026286

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014214
Physical Location & Seq
Reverse (-)
1890235 .. 1894349
4115 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026286

Sequence Viewer

Length: 1296 bp
ATGTTCCAACCCAACAACAACAACCAGCTTCCAATTAAGTCATTACCTATTCAAGATCCATCAGCACTTGTGCAAGTGGATAAAAAAGAAACTAGCGTAGTCTTGAAGGATGAAACGCAAAACGGAGGATTCGCGCAAGCAGAATCTATCATCTTCTACACTAAGAGACTTCAAGATGATTTGCATGTGATGGGGTTGAAAGTAAAACAGCATGAGGAGAACATGAAACTTCTGAGGTCTCAGAAAAACAAATTAGAAGAATCTATTCTTGATCTGCAAGTTATGTTAGGCAACTATCATTCCTCAACCACAACTACTACTGCAAATGATAACCATAGTCAGGAGGAAACAACTAAAAAGATTCTTCAGTGCGAGAAGTCAGCTGCAGGCATTTTATGGCAGCTGAAAGCTCGTCATGGTAATCAGGCTGCGGATCTTCCCCTGACCAAGGATATTGTGGGTATTGTTGCGATGCTTGGGAAAGTAGATGATGATAATCTTAGCAGGCTTTTGTCAGAGTACTTAGGACTGGAGACTATGTTAGCAATTGTCTGCAAGACTTATGATGGTGTCAGAGCTTTAGAATTGTATGACAATGAAGGCTGCATTAACCTGAATTCCGGTCTTCACGGGCTTGGTACTTCTATTGGGAGGAAATTGGAAGGTCGGTTTCTGGTCATTTGTCTTGAAAATTTAAGACCATATGCTGGTGAGTTTGTACATAATGACCCCCAACGGAGGCTTGATATTCTAAAACCAAGGTTGCCGAATGGGGAGTGTCCACCTGGATTTCTTGGCTATGCTGTAAATATGGTCAATGTGGATAGCACAAATTTATTTTGTCTCACAGCCAGTGGACACGGCCTGAGAGAGACTCTATTTTATAGCCTCTTTTCTCGCTTGCAAGTGTATAAAACAAGAGCAGATATGGTATCTGCTCTTCCTTGTATAACTGATGGAGCCATTTCTTTAGATGGTGGGATGATTCAGAGGACTGGTGTGTTTTCCCTTGGGAACAGGGAAGATGTAGATGTGAAGTTTCCAAAGCTCTCAGCGGCATCAGGCCTGCCCGAAAGCTATCTAGAAACAGAAAAACAAATCAATGAGTTGAAATGGAGAAATGAAAAATTGATTGAAGATACGAAGAGAGAACAGGCATTATGGGACAATGCAAAATTCAATTTTGACAGAAAGAAAAAAGAGTTCCTCAAGTTCCTGGCTGATAGTTCATCGTATGCAACTCAGGCAACTCAGCATCAAATTATGGGGGCGCAGAATAGATTTACCTCGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

432

Amino Acids

48.31

Weight (kDa)

6.34

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AccII CGCG 1 cut(s) 134
AciI CCGC 2 cut(s) 431, 1055
AclWI GGATC 2 cut(s) 50, 441
AcsI RAATTY 4 cut(s) 616, 691, 832, 1175
AcuI CTGAAG 1 cut(s) 350
AfaI GTAC 3 cut(s) 521, 640, 720
AfiI CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
AgsI TTSAA 8 cut(s) 53, 106, 173, 199, 689, 1111, 1136, 1180
AjnI CCWGG 2 cut(s) 784, 1215
AluBI AGCT 8 cut(s) 28, 383, 403, 410, 578, 1048, 1077, 1293
AluI AGCT 8 cut(s) 28, 383, 403, 410, 578, 1048, 1077, 1293
Alw26I GTCTC 5 cut(s) 160, 243, 527, 848, 866
AlwI GGATC 2 cut(s) 50, 441
Ama87I CYCGRG 1 cut(s) 1288
AoxI GGCC 2 cut(s) 862, 1063
ApeKI GCWGC 4 cut(s) 383, 400, 428, 603
ApoI RAATTY 4 cut(s) 616, 691, 832, 1175
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 2 cut(s) 136, 1273
AsuHPI GGTGA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 1288
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BbsI GAAGAC 1 cut(s) 617
BbvI GCAGC 4 cut(s) 370, 412, 415, 590
BccI CCATC 5 cut(s) 67, 184, 560, 950, 968
BceAI ACGGC 1 cut(s) 877
BciT130I CCWGG 2 cut(s) 786, 1217
BcoDI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BfaI CTAG 2 cut(s) 93, 1082
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 5 cut(s) 384, 401, 429, 604, 1056
BlsI GCNGC 5 cut(s) 385, 402, 430, 605, 1057
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 2 cut(s) 786, 1217
BmeT110I CYCGRG 1 cut(s) 1288
BmiI GGNNCC 1 cut(s) 961
BmrFI CCNGG 2 cut(s) 786, 1217
BmsI GCATC 3 cut(s) 462, 1067, 1264
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 859, 891
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 1 cut(s) 1193
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 3 cut(s) 447, 758, 1009
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
Bse1I ACTGG 3 cut(s) 534, 852, 1000
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 2 cut(s) 786, 1217
BseDI CCNNGG 3 cut(s) 447, 758, 1009
BseGI GGATG 2 cut(s) 115, 987
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
BseMII CTCAG 6 cut(s) 224, 254, 857, 1065, 1256, 1265
BseNI ACTGG 3 cut(s) 534, 852, 1000
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 4 cut(s) 370, 412, 415, 590
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 2 cut(s) 864, 1065
BsiHKCI CYCGRG 1 cut(s) 1288
BsiSI CCGG 1 cut(s) 621
BslFI GGGAC 1 cut(s) 1178
BslI CCNNNNNNNGG 4 cut(s) 340, 448, 707, 738
BsmAI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BsmFI GGGAC 1 cut(s) 1178
BsnI GGCC 2 cut(s) 864, 1065
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 1288
Bsp1407I TGTACA 1 cut(s) 718
Bsp143I GATC 3 cut(s) 55, 271, 433
BspACI CCGC 2 cut(s) 431, 1055
BspANI GGCC 2 cut(s) 864, 1065
BspCNI CTCAG 6 cut(s) 225, 253, 858, 1064, 1255, 1264
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 1 cut(s) 961
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 2 cut(s) 50, 441
BspQI GCTCTTC 1 cut(s) 945
BspTNI GGTCTC 1 cut(s) 243
BsrGI TGTACA 1 cut(s) 718
BsrI ACTGG 3 cut(s) 534, 852, 1000
BssECI CCNNGG 3 cut(s) 447, 758, 1009
BssMI GATC 3 cut(s) 55, 271, 433
BssT1I CCWWGG 3 cut(s) 447, 758, 1009
Bst2UI CCWGG 2 cut(s) 786, 1217
Bst6I CTCTTC 2 cut(s) 945, 1139
BstAUI TGTACA 1 cut(s) 718
BstC8I GCNNGC 5 cut(s) 138, 388, 506, 902, 1067
BstDEI CTNAG 9 cut(s) 162, 233, 240, 500, 523, 866, 1051, 1242, 1251
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 2 cut(s) 115, 987
BstFNI CGCG 1 cut(s) 134
BstHHI GCGC 2 cut(s) 136, 1273
BstKTI GATC 3 cut(s) 58, 274, 436
BstMAI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BstMBI GATC 3 cut(s) 55, 271, 433
BstMWI GCNNNNNNNGC 1 cut(s) 1244
BstNI CCWGG 2 cut(s) 786, 1217
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 784, 1215
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 4 cut(s) 370, 412, 415, 590
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 2 cut(s) 55, 433
BstYI RGATCY 2 cut(s) 55, 433
BsuRI GGCC 2 cut(s) 864, 1065
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 2 cut(s) 115, 987
BtsIMutI CAGTG 2 cut(s) 374, 859
Cac8I GCNNGC 5 cut(s) 138, 388, 506, 902, 1067
CfoI GCGC 2 cut(s) 136, 1273
Csp6I GTAC 3 cut(s) 520, 639, 719
CviAII CATG 4 cut(s) 185, 212, 223, 416
CviQI GTAC 3 cut(s) 520, 639, 719
DdeI CTNAG 9 cut(s) 162, 233, 240, 500, 523, 866, 1051, 1242, 1251
DpnI GATC 3 cut(s) 57, 273, 435
DpnII GATC 3 cut(s) 55, 271, 433
Eam1104I CTCTTC 2 cut(s) 945, 1139
EarI CTCTTC 2 cut(s) 945, 1139
Eco130I CCWWGG 3 cut(s) 447, 758, 1009
Eco147I AGGCCT 1 cut(s) 1065
Eco31I GGTCTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 1288
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 616
EcoRII CCWGG 2 cut(s) 784, 1215
EcoT14I CCWWGG 3 cut(s) 447, 758, 1009
ErhI CCWWGG 3 cut(s) 447, 758, 1009
FaeI CATG 4 cut(s) 188, 215, 226, 419
FaqI GGGAC 1 cut(s) 1178
FatI CATG 4 cut(s) 184, 211, 222, 415
FauNDI CATATG 1 cut(s) 703
Fnu4HI GCNGC 5 cut(s) 384, 401, 429, 604, 1056
FokI GGATG 2 cut(s) 122, 994
Fsp4HI GCNGC 5 cut(s) 384, 401, 429, 604, 1056
FspBI CTAG 2 cut(s) 93, 1082
GlaI GCGC 2 cut(s) 135, 1272
GluI GCNGC 5 cut(s) 384, 401, 429, 604, 1056
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 2 cut(s) 864, 1065
HapII CCGG 1 cut(s) 621
HhaI GCGC 2 cut(s) 136, 1273
Hin1II CATG 4 cut(s) 188, 215, 226, 419
Hin6I GCGC 2 cut(s) 134, 1271
HinP1I GCGC 2 cut(s) 134, 1271
HinfI GANTC 6 cut(s) 129, 143, 260, 361, 874, 985
HpaII CCGG 1 cut(s) 621
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 2 cut(s) 782, 857
Hpy188I TCNGA 5 cut(s) 234, 243, 517, 575, 990
Hpy188III TCNNGA 7 cut(s) 53, 103, 173, 269, 341, 686, 1082
Hpy8I GTNNAC 2 cut(s) 782, 857
HpyAV CCTTC 3 cut(s) 100, 593, 656
HpyF10VI GCNNNNNNNGC 1 cut(s) 1244
HpyF3I CTNAG 9 cut(s) 162, 233, 240, 500, 523, 866, 1051, 1242, 1251
Hsp92II CATG 4 cut(s) 188, 215, 226, 419
HspAI GCGC 2 cut(s) 134, 1271
Kzo9I GATC 3 cut(s) 55, 271, 433
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 1 cut(s) 959
Lsp1109I GCAGC 4 cut(s) 370, 412, 415, 590
LweI GCATC 3 cut(s) 462, 1067, 1264
MaeI CTAG 2 cut(s) 93, 1082
MalI GATC 3 cut(s) 57, 273, 435
MboI GATC 3 cut(s) 55, 271, 433
MboII GAAGA 9 cut(s) 145, 269, 356, 428, 617, 932, 1034, 1148, 1156
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 2 cut(s) 55, 433
MlyI GAGTC 1 cut(s) 868
MmeI TCCRAC 1 cut(s) 31
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 3 cut(s) 36, 609, 695
MspA1I CMGCKG 3 cut(s) 383, 403, 1055
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 2 cut(s) 786, 1217
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 2 cut(s) 786, 1217
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 1244
NdeI CATATG 1 cut(s) 703
NdeII GATC 3 cut(s) 55, 271, 433
NlaIII CATG 4 cut(s) 188, 215, 226, 419
NlaIV GGNNCC 1 cut(s) 961
NspI RCATGY 1 cut(s) 188
PaeR7I CTCGAG 1 cut(s) 1288
PceI AGGCCT 1 cut(s) 1065
PciSI GCTCTTC 1 cut(s) 945
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 5 cut(s) 129, 143, 260, 361, 985
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 5 cut(s) 385, 402, 430, 605, 1057
PleI GAGTC 1 cut(s) 868
PpsI GAGTC 1 cut(s) 868
Psp6I CCWGG 2 cut(s) 784, 1215
PspGI CCWGG 2 cut(s) 784, 1215
PspN4I GGNNCC 1 cut(s) 961
PspXI VCTCGAGB 1 cut(s) 1288
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 2 cut(s) 55, 433
PvuII CAGCTG 2 cut(s) 383, 403
RsaI GTAC 3 cut(s) 521, 640, 720
RsaNI GTAC 3 cut(s) 520, 639, 719
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 3 cut(s) 36, 609, 695
SatI GCNGC 5 cut(s) 384, 401, 429, 604, 1056
Sau3AI GATC 3 cut(s) 55, 271, 433
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 1 cut(s) 868
ScrFI CCNGG 2 cut(s) 786, 1217
SfaNI GCATC 3 cut(s) 462, 1067, 1264
SfcI CTRYAG 1 cut(s) 384
Sfr274I CTCGAG 1 cut(s) 1288
SlaI CTCGAG 1 cut(s) 1288
SmlI CTYRAG 2 cut(s) 1208, 1288
SmoI CTYRAG 2 cut(s) 1208, 1288
SseBI AGGCCT 1 cut(s) 1065
SsiI CCGC 2 cut(s) 431, 1055
SspMI CTAG 2 cut(s) 93, 1082
StuI AGGCCT 1 cut(s) 1065
StyD4I CCNGG 2 cut(s) 784, 1215
StyI CCWWGG 3 cut(s) 447, 758, 1009
TaqI TCGA 1 cut(s) 1289
TatI WGTACW 2 cut(s) 519, 718
TauI GCSGC 1 cut(s) 1058
TfiI GAWTC 5 cut(s) 129, 143, 260, 361, 985
Tru1I TTAA 3 cut(s) 36, 609, 695
Tru9I TTAA 3 cut(s) 36, 609, 695
TscAI CASTG 2 cut(s) 374, 859
TseI GCWGC 4 cut(s) 383, 400, 428, 603
TspDTI ATGAA 5 cut(s) 126, 239, 612, 1137, 1218
TspGWI ACGGA 2 cut(s) 138, 751
TspRI CASTG 2 cut(s) 374, 859
Van91I CCANNNNNTGG 1 cut(s) 707
XagI CCTNNNNNAGG 1 cut(s) 446
XapI RAATTY 4 cut(s) 616, 691, 832, 1175
XbaI TCTAGA 1 cut(s) 1081
XceI RCATGY 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 1288
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 2 cut(s) 93, 1082
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.