Rmu_ssc0000015.1_g000009

gamma-irradiation and mitomycin c induced

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000015.1
OG0014214
Physical Location & Seq
Forward (+)
35359 .. 40708
5350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000015.1_g000009.1.cds

Sequence Viewer

Length: 1668 bp
atgttccaacccaacaacaacaaccagcttccaatcaagtcattacctattcaagatccatcagcaccgatgcaagtggataaaaaagaaactagcatagtcttgaaggatgaaacgcagaacggaggattcgcgcaagcagaatctatcatcttctacactaagagacttcaagatgatttgcatgagatggggttgaaagtcaaacagcatgaggaggacatgaaacttctgaggtctcagaaaaacaaattagaagaatctattcttgatctgcaagttatgttaggcaactatcattcctcaaccacaactactactgcaaatgataaccatagtcaggaggaagcaattaaaaagattcttcagtgcgagaagtcagctgcaggcattttatggcagctgaaagctcgtcatggtaatcaggctgcggatcttcccctgaccaaggatattgtgggtattgttgcgatgcttgggaaagtagatgatgataatcttagcaggcttttgtcagagtacttgggactggagactatgttagcaattgtctgcaagacttatgatggtgtcagagctctagaattgtatgacaacgaaggttgcataaacctgaattccggtcttcacgggcttggtacttctattgggaggaaattggaaggtcggtttctggtcatttgtcttgaaaatttaagaccatatgctggtgagtttgtacataatgacccccaacggaggcttgatattctaaaaccaaggttgctgaatggggagtgtccacctggatttcttggctatgctgtaaatatgatcaatgtggatagcacaaatttattttgtctcacagccagtggacacggcctgagagagactctactttatagcctcttttctcgcttgcaagtgtataaaacaagagcagatatggtatctgctcttccttgtataactgatggagccatttctttagatggtgggatgattcagaggactggtgtgttttcccttggcaacagggaagacgtaggtgtgaagtttccaaagctctcagtggcatcaggcctgcccgaaagctatctagaaacagaaagacaaatcaatgagtcgaaatggagaaaagaaaaattgattgaagatacgaagagagtacaggcattatgggacaatgcaaaattcaattttgatagaaagaaaaaagagttcctcaagttcctggctgatagttcatcttatgcaactcaggcaactcagttgacgagaaccgtaggaagtaaatcaatggctggccaagctgcaaagtcagtggcaaaggcaattggggagtaccagtacccatggcgcgagaagttggtaaaataccagtctgagctatccaagggtgtatggggttactggcatctaggagcctggaagccatctggaattagtggtcgtcaacgagctagacttcgaaaggaagtcctccttgcaggacaggattggccatatgatccagagaggaaagagatgaggaccaagaggaaagggcacaaagttgataggatagctgccgagaagcgggaaaacactgccaggttgatggagaagatgcccgatatgttgcttcagtacaagaagcgcagatgggagaagaagatgaaggaagaagacaagggcaagccctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c_3

Protein Analysis

555

Amino Acids

62.96

Weight (kDa)

9.21

Isoelectric Point (pI)

37.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G49250
fragaria_vesca FvH4_7g31551
malus_domestica MD01G1218300.v1.1 MD07G1288200.v1.1
prunus_persica Prupe.2G309700_v2.0.a1
pyrus_communis pycom01g22830 pycom07g26100
rosa_chinensis RchiOBHm_Chr1g0380541
rosa_laevigata RLG00000026286
rosa_multiflora Rmu_co8407499.1_g000001 Rmu_ssc0000015.1_g000009
rosa_roxburghii Rroxscaffold_4G00279210
rosa_rugosa Rorug01G0422400
rosa_samantha Rh1AG446900 Rh1BG402200 Rh1CG416400 Rh1DG432400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 707
AccII CGCG 2 cut(s) 134, 1344
AciI CCGC 2 cut(s) 431, 1561
AclWI GGATC 3 cut(s) 50, 441, 1487
AcoI YGGCCR 2 cut(s) 1288, 1484
AcsI RAATTY 4 cut(s) 616, 691, 832, 1175
AcuI CTGAAG 2 cut(s) 350, 1592
AfaI GTAC 7 cut(s) 521, 640, 720, 1152, 1328, 1334, 1613
AfiI CCNNNNNNNGG 5 cut(s) 340, 448, 707, 738, 1560
AgsI TTSAA 7 cut(s) 53, 106, 173, 199, 689, 1136, 1180
AjnI CCWGG 4 cut(s) 784, 1215, 1409, 1574
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 5 cut(s) 160, 243, 527, 848, 866
AlwI GGATC 3 cut(s) 50, 441, 1487
AoxI GGCC 4 cut(s) 862, 1063, 1288, 1484
ApeKI GCWGC 5 cut(s) 383, 400, 428, 1295, 1550
ApoI RAATTY 4 cut(s) 616, 691, 832, 1175
Asp700I GAANNNNTTC 1 cut(s) 264
AspLEI GCGC 3 cut(s) 136, 1344, 1623
AspS9I GGNCC 1 cut(s) 1515
AsuHPI GGTGA 1 cut(s) 722
AsuII TTCGAA 1 cut(s) 1453
AvaII GGWCC 1 cut(s) 1515
BaeGI GKGCMC 1 cut(s) 1533
BalI TGGCCA 2 cut(s) 1290, 1486
BanII GRGCYC 1 cut(s) 580
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BbsI GAAGAC 3 cut(s) 617, 1029, 1656
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 5 cut(s) 370, 412, 415, 1282, 1537
BccI CCATC 8 cut(s) 67, 184, 560, 950, 968, 1426, 1576, 1620
BceAI ACGGC 1 cut(s) 877
BciT130I CCWGG 4 cut(s) 786, 1217, 1411, 1576
BclI TGATCA 1 cut(s) 813
BcoDI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BfaI CTAG 5 cut(s) 93, 581, 1082, 1403, 1446
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 5 cut(s) 384, 401, 429, 1296, 1551
BlsI GCNGC 5 cut(s) 385, 402, 430, 1297, 1552
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 4 cut(s) 786, 1217, 1411, 1576
Bme18I GGWCC 1 cut(s) 1515
BmgT120I GGNCC 1 cut(s) 1515
BmiI GGNNCC 2 cut(s) 961, 1408
BmrFI CCNGG 4 cut(s) 786, 1217, 1411, 1576
BmsI GCATC 5 cut(s) 60, 462, 1067, 1408, 1581
BpiI GAAGAC 3 cut(s) 617, 1029, 1656
BplI GAGNNNNNCTC 2 cut(s) 859, 891
BpmI CTGGAG 1 cut(s) 551
Bpu14I TTCGAA 1 cut(s) 1453
BpuEI CTTGAG 1 cut(s) 1193
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 5 cut(s) 447, 758, 1009, 1337, 1377
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 4 cut(s) 524, 554, 1316, 1346
Bsc4I CCNNNNNNNGG 5 cut(s) 340, 448, 707, 738, 1560
Bse1I ACTGG 6 cut(s) 534, 852, 1000, 1330, 1363, 1400
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 4 cut(s) 786, 1217, 1411, 1576
BseDI CCNNGG 5 cut(s) 447, 758, 1009, 1337, 1377
BseGI GGATG 2 cut(s) 115, 987
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 5 cut(s) 340, 448, 707, 738, 1560
BseMII CTCAG 7 cut(s) 224, 254, 857, 1065, 1256, 1265, 1359
BseNI ACTGG 6 cut(s) 534, 852, 1000, 1330, 1363, 1400
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 1 cut(s) 1533
BseXI GCAGC 5 cut(s) 370, 412, 415, 1282, 1537
Bsh1236I CGCG 2 cut(s) 134, 1344
BshFI GGCC 4 cut(s) 864, 1065, 1290, 1486
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 1 cut(s) 621
BslFI GGGAC 2 cut(s) 540, 1178
BslI CCNNNNNNNGG 5 cut(s) 340, 448, 707, 738, 1560
BsmAI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BsmFI GGGAC 2 cut(s) 540, 1178
BsnI GGCC 4 cut(s) 864, 1065, 1290, 1486
Bso31I GGTCTC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 1453
Bsp1286I GDGCHC 2 cut(s) 580, 1533
Bsp1407I TGTACA 1 cut(s) 718
Bsp143I GATC 5 cut(s) 55, 271, 433, 813, 1492
Bsp19I CCATGG 1 cut(s) 1337
BspACI CCGC 2 cut(s) 431, 1561
BspANI GGCC 4 cut(s) 864, 1065, 1290, 1486
BspCNI CTCAG 7 cut(s) 225, 253, 858, 1064, 1255, 1264, 1360
BspFNI CGCG 2 cut(s) 134, 1344
BspLI GGNNCC 2 cut(s) 961, 1408
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 3 cut(s) 50, 441, 1487
BspQI GCTCTTC 1 cut(s) 945
BspT104I TTCGAA 1 cut(s) 1453
BspTNI GGTCTC 1 cut(s) 243
BsrGI TGTACA 1 cut(s) 718
BsrI ACTGG 6 cut(s) 534, 852, 1000, 1330, 1363, 1400
BssECI CCNNGG 5 cut(s) 447, 758, 1009, 1337, 1377
BssMI GATC 5 cut(s) 55, 271, 433, 813, 1492
BssT1I CCWWGG 5 cut(s) 447, 758, 1009, 1337, 1377
Bst2UI CCWGG 4 cut(s) 786, 1217, 1411, 1576
Bst4CI ACNGT 1 cut(s) 1267
Bst6I CTCTTC 2 cut(s) 945, 1139
BstAUI TGTACA 1 cut(s) 718
BstBI TTCGAA 1 cut(s) 1453
BstC8I GCNNGC 7 cut(s) 138, 388, 506, 902, 1067, 1288, 1661
BstDEI CTNAG 9 cut(s) 162, 233, 240, 500, 866, 1051, 1242, 1251, 1368
BstDSI CCRYGG 1 cut(s) 1337
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 2 cut(s) 115, 987
BstFNI CGCG 2 cut(s) 134, 1344
BstHHI GCGC 3 cut(s) 136, 1344, 1623
BstKTI GATC 5 cut(s) 58, 274, 436, 816, 1495
BstMAI GTCTC 5 cut(s) 160, 243, 527, 848, 866
BstMBI GATC 5 cut(s) 55, 271, 433, 813, 1492
BstMWI GCNNNNNNNGC 2 cut(s) 1244, 1292
BstNI CCWGG 4 cut(s) 786, 1217, 1411, 1576
BstSCI CCNGG 4 cut(s) 784, 1215, 1409, 1574
BstSFI CTRYAG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 1533
BstUI CGCG 2 cut(s) 134, 1344
BstV1I GCAGC 5 cut(s) 370, 412, 415, 1282, 1537
BstV2I GAAGAC 3 cut(s) 617, 1029, 1656
BstX2I RGATCY 2 cut(s) 55, 433
BstXI CCANNNNNNTGG 1 cut(s) 1582
BstYI RGATCY 2 cut(s) 55, 433
BsuRI GGCC 4 cut(s) 864, 1065, 1290, 1486
BtgI CCRYGG 1 cut(s) 1337
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 2 cut(s) 115, 987
BtsI GCAGTG 1 cut(s) 1569
BtsIMutI CAGTG 5 cut(s) 374, 859, 1059, 1311, 1569
Cac8I GCNNGC 7 cut(s) 138, 388, 506, 902, 1067, 1288, 1661
CfoI GCGC 3 cut(s) 136, 1344, 1623
Cfr13I GGNCC 1 cut(s) 1515
Csp6I GTAC 7 cut(s) 520, 639, 719, 1151, 1327, 1333, 1612
CspCI CAANNNNNGTGG 2 cut(s) 1287, 1322
CviAII CATG 5 cut(s) 185, 212, 223, 416, 1338
CviQI GTAC 7 cut(s) 520, 639, 719, 1151, 1327, 1333, 1612
DdeI CTNAG 9 cut(s) 162, 233, 240, 500, 866, 1051, 1242, 1251, 1368
DpnI GATC 5 cut(s) 57, 273, 435, 815, 1494
DpnII GATC 5 cut(s) 55, 271, 433, 813, 1492
EaeI YGGCCR 2 cut(s) 1288, 1484
Eam1104I CTCTTC 2 cut(s) 945, 1139
EarI CTCTTC 2 cut(s) 945, 1139
Ecl136II GAGCTC 1 cut(s) 578
Eco130I CCWWGG 5 cut(s) 447, 758, 1009, 1337, 1377
Eco147I AGGCCT 1 cut(s) 1065
Eco24I GRGCYC 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 1515
Eco53kI GAGCTC 1 cut(s) 578
Eco57I CTGAAG 2 cut(s) 350, 1592
EcoICRI GAGCTC 1 cut(s) 578
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRI GAATTC 1 cut(s) 616
EcoRII CCWGG 4 cut(s) 784, 1215, 1409, 1574
EcoT14I CCWWGG 5 cut(s) 447, 758, 1009, 1337, 1377
EcoT38I GRGCYC 1 cut(s) 580
ErhI CCWWGG 5 cut(s) 447, 758, 1009, 1337, 1377
FaeI CATG 5 cut(s) 188, 215, 226, 419, 1341
FalI AAGNNNNNCTT 2 cut(s) 1452, 1484
FaqI GGGAC 2 cut(s) 540, 1178
FatI CATG 5 cut(s) 184, 211, 222, 415, 1337
FauI CCCGC 1 cut(s) 1554
FauNDI CATATG 2 cut(s) 703, 1489
FbaI TGATCA 1 cut(s) 813
Fnu4HI GCNGC 5 cut(s) 384, 401, 429, 1296, 1551
FokI GGATG 2 cut(s) 122, 994
FriOI GRGCYC 1 cut(s) 580
Fsp4HI GCNGC 5 cut(s) 384, 401, 429, 1296, 1551
FspBI CTAG 5 cut(s) 93, 581, 1082, 1403, 1446
GlaI GCGC 3 cut(s) 135, 1343, 1622
GluI GCNGC 5 cut(s) 384, 401, 429, 1296, 1551
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 4 cut(s) 864, 1065, 1290, 1486
HapII CCGG 1 cut(s) 621
HhaI GCGC 3 cut(s) 136, 1344, 1623
Hin1II CATG 5 cut(s) 188, 215, 226, 419, 1341
Hin6I GCGC 3 cut(s) 134, 1342, 1621
HinP1I GCGC 3 cut(s) 134, 1342, 1621
HincII GTYRAC 2 cut(s) 1257, 1439
HindII GTYRAC 2 cut(s) 1257, 1439
HinfI GANTC 7 cut(s) 129, 143, 260, 361, 874, 985, 1106
HpaII CCGG 1 cut(s) 621
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 4 cut(s) 782, 857, 1257, 1439
Hpy188I TCNGA 6 cut(s) 234, 243, 517, 575, 990, 1369
Hpy8I GTNNAC 4 cut(s) 782, 857, 1257, 1439
HpyAV CCTTC 4 cut(s) 100, 593, 656, 1636
HpyCH4III ACNGT 1 cut(s) 1267
HpyCH4IV ACGT 1 cut(s) 1026
HpyF10VI GCNNNNNNNGC 2 cut(s) 1244, 1292
HpyF3I CTNAG 9 cut(s) 162, 233, 240, 500, 866, 1051, 1242, 1251, 1368
HpySE526I ACGT 1 cut(s) 1026
Hsp92II CATG 5 cut(s) 188, 215, 226, 419, 1341
HspAI GCGC 3 cut(s) 134, 1342, 1621
Ksp22I TGATCA 1 cut(s) 813
Kzo9I GATC 5 cut(s) 55, 271, 433, 813, 1492
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 2 cut(s) 959, 1406
Lsp1109I GCAGC 5 cut(s) 370, 412, 415, 1282, 1537
LweI GCATC 5 cut(s) 60, 462, 1067, 1408, 1581
MaeI CTAG 5 cut(s) 93, 581, 1082, 1403, 1446
MaeII ACGT 1 cut(s) 1026
MaeIII GTNAC 1 cut(s) 1391
MalI GATC 5 cut(s) 57, 273, 435, 815, 1494
MboI GATC 5 cut(s) 55, 271, 433, 813, 1492
MfeI CAATTG 2 cut(s) 546, 1317
MflI RGATCY 2 cut(s) 55, 433
MhlI GDGCHC 2 cut(s) 580, 1533
MlsI TGGCCA 2 cut(s) 1290, 1486
MluNI TGGCCA 2 cut(s) 1290, 1486
MlyI GAGTC 2 cut(s) 868, 1115
MmeI TCCRAC 1 cut(s) 31
Mox20I TGGCCA 2 cut(s) 1290, 1486
MroXI GAANNNNTTC 1 cut(s) 264
MscI TGGCCA 2 cut(s) 1290, 1486
MseI TTAA 2 cut(s) 354, 695
Msp20I TGGCCA 2 cut(s) 1290, 1486
MspA1I CMGCKG 2 cut(s) 383, 403
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 4 cut(s) 786, 1217, 1411, 1576
MunI CAATTG 2 cut(s) 546, 1317
MvaI CCWGG 4 cut(s) 786, 1217, 1411, 1576
MvnI CGCG 2 cut(s) 134, 1344
MwoI GCNNNNNNNGC 2 cut(s) 1244, 1292
NcoI CCATGG 1 cut(s) 1337
NdeI CATATG 2 cut(s) 703, 1489
NdeII GATC 5 cut(s) 55, 271, 433, 813, 1492
NlaIII CATG 5 cut(s) 188, 215, 226, 419, 1341
NlaIV GGNNCC 2 cut(s) 961, 1408
NmeAIII GCCGAG 1 cut(s) 1579
NspV TTCGAA 1 cut(s) 1453
PceI AGGCCT 1 cut(s) 1065
PciSI GCTCTTC 1 cut(s) 945
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 5 cut(s) 129, 143, 260, 361, 985
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 5 cut(s) 385, 402, 430, 1297, 1552
PleI GAGTC 2 cut(s) 868, 1114
PpsI GAGTC 2 cut(s) 868, 1114
Psp124BI GAGCTC 1 cut(s) 580
Psp6I CCWGG 4 cut(s) 784, 1215, 1409, 1574
PspGI CCWGG 4 cut(s) 784, 1215, 1409, 1574
PspN4I GGNNCC 2 cut(s) 961, 1408
PspPI GGNCC 1 cut(s) 1515
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 2 cut(s) 55, 433
PvuII CAGCTG 2 cut(s) 383, 403
RsaI GTAC 7 cut(s) 521, 640, 720, 1152, 1328, 1334, 1613
RsaNI GTAC 7 cut(s) 520, 639, 719, 1151, 1327, 1333, 1612
SacI GAGCTC 1 cut(s) 580
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 2 cut(s) 354, 695
SatI GCNGC 5 cut(s) 384, 401, 429, 1296, 1551
Sau3AI GATC 5 cut(s) 55, 271, 433, 813, 1492
Sau96I GGNCC 1 cut(s) 1515
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 2 cut(s) 868, 1115
ScrFI CCNGG 4 cut(s) 786, 1217, 1411, 1576
SduI GDGCHC 2 cut(s) 580, 1533
SfaNI GCATC 5 cut(s) 60, 462, 1067, 1408, 1581
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 1453
SinI GGWCC 1 cut(s) 1515
SmlI CTYRAG 1 cut(s) 1208
SmoI CTYRAG 1 cut(s) 1208
SseBI AGGCCT 1 cut(s) 1065
SsiI CCGC 2 cut(s) 431, 1561
SspMI CTAG 5 cut(s) 93, 581, 1082, 1403, 1446
SstI GAGCTC 1 cut(s) 580
StuI AGGCCT 1 cut(s) 1065
StyD4I CCNGG 4 cut(s) 784, 1215, 1409, 1574
StyI CCWWGG 5 cut(s) 447, 758, 1009, 1337, 1377
TaaI ACNGT 1 cut(s) 1267
TaiI ACGT 1 cut(s) 1029
TaqI TCGA 2 cut(s) 1109, 1453
TatI WGTACW 4 cut(s) 519, 718, 1150, 1611
TfiI GAWTC 5 cut(s) 129, 143, 260, 361, 985
Tru1I TTAA 2 cut(s) 354, 695
Tru9I TTAA 2 cut(s) 354, 695
TscAI CASTG 5 cut(s) 374, 859, 1059, 1311, 1576
TseI GCWGC 5 cut(s) 383, 400, 428, 1295, 1550
TspDTI ATGAA 4 cut(s) 126, 239, 1218, 1655
TspGWI ACGGA 2 cut(s) 138, 751
TspRI CASTG 5 cut(s) 374, 859, 1059, 1311, 1576
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 1515
XagI CCTNNNNNAGG 1 cut(s) 446
XapI RAATTY 4 cut(s) 616, 691, 832, 1175
XbaI TCTAGA 2 cut(s) 580, 1081
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 5 cut(s) 93, 581, 1082, 1403, 1446
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.