Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G64160 AT4G23690 |
| fragaria_vesca | FvH4_5g03711 FvH4_5g03720 |
| malus_domestica | MD06G1096600.v1.1 MD14G1116900.v1.1 |
| prunus_persica | Prupe.5G111400_v2.0.a1 |
| rosa_chinensis | RchiOBHm_Chr3g0480301 RchiOBHm_Chr7g0195991 RchiOBHm_Chr7g0196001 |
| rosa_laevigata | RLG00000004076 RLG00000023497 |
| rosa_multiflora | Rmu_sc0000311.1_g000009 Rmu_sc0001640.1_g000015 Rmu_sc0005575.1_g000001 |
| rosa_roxburghii | Rroxscaffold_3G00259960 |
| rosa_rugosa | Rorug07G0028700 Rorug07G0028900 |
| rosa_samantha | Rh3AG233800 Rh3BG266100 Rh3CG263800 Rh7AG155400 Rh7BG157300 Rh7BG157400 Rh7CG160800 Rh7DG156400 Rh7DG156500 |
| rosa_wichuraiana | Rw3G021010 Rw7G013290 Rw7G013300 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 176 |
| AccB1I | GGYRCC | 1 cut(s) | 395 |
| AccII | CGCG | 1 cut(s) | 185 |
| AciI | CCGC | 2 cut(s) | 183, 185 |
| AclWI | GGATC | 1 cut(s) | 432 |
| AcoI | YGGCCR | 1 cut(s) | 186 |
| AcsI | RAATTY | 1 cut(s) | 83 |
| AcuI | CTGAAG | 1 cut(s) | 542 |
| AcyI | GRCGYC | 2 cut(s) | 173, 396 |
| AfaI | GTAC | 1 cut(s) | 344 |
| AgsI | TTSAA | 2 cut(s) | 358, 385 |
| AleI | CACNNNNGTG | 1 cut(s) | 350 |
| AluBI | AGCT | 4 cut(s) | 27, 136, 202, 290 |
| AluI | AGCT | 4 cut(s) | 27, 136, 202, 290 |
| Alw21I | GWGCWC | 2 cut(s) | 292, 372 |
| Alw26I | GTCTC | 1 cut(s) | 444 |
| AlwI | GGATC | 1 cut(s) | 432 |
| AoxI | GGCC | 2 cut(s) | 39, 186 |
| ApoI | RAATTY | 1 cut(s) | 83 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 122, 154, 200, 232, 478, 510 |
| AspLEI | GCGC | 1 cut(s) | 398 |
| AspS9I | GGNCC | 2 cut(s) | 40, 444 |
| AvaII | GGWCC | 1 cut(s) | 444 |
| BaeGI | GKGCMC | 1 cut(s) | 380 |
| BanI | GGYRCC | 1 cut(s) | 395 |
| BanII | GRGCYC | 1 cut(s) | 292 |
| Bbv12I | GWGCWC | 2 cut(s) | 292, 372 |
| BccI | CCATC | 4 cut(s) | 64, 106, 260, 520 |
| BceAI | ACGGC | 2 cut(s) | 160, 345 |
| BcoDI | GTCTC | 1 cut(s) | 444 |
| BfaI | CTAG | 2 cut(s) | 420, 465 |
| BfoI | RGCGCY | 1 cut(s) | 399 |
| BisI | GCNGC | 1 cut(s) | 186 |
| BlpI | GCTNAGC | 1 cut(s) | 203 |
| BlsI | GCNGC | 1 cut(s) | 187 |
| Bme18I | GGWCC | 1 cut(s) | 444 |
| BmgT120I | GGNCC | 2 cut(s) | 40, 444 |
| BmiI | GGNNCC | 2 cut(s) | 397, 445 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 203 |
| BpuEI | CTTGAG | 2 cut(s) | 276, 346 |
| BsaBI | GATNNNNATC | 1 cut(s) | 530 |
| BsaHI | GRCGYC | 2 cut(s) | 173, 396 |
| BsaJI | CCNNGG | 2 cut(s) | 183, 279 |
| BsaWI | WCCGGW | 1 cut(s) | 446 |
| Bse8I | GATNNNNATC | 1 cut(s) | 530 |
| BseDI | CCNNGG | 2 cut(s) | 183, 279 |
| BseGI | GGATG | 1 cut(s) | 117 |
| BseJI | GATNNNNATC | 1 cut(s) | 530 |
| BseMII | CTCAG | 1 cut(s) | 217 |
| BseSI | GKGCMC | 1 cut(s) | 380 |
| Bsh1236I | CGCG | 1 cut(s) | 185 |
| BshFI | GGCC | 2 cut(s) | 41, 188 |
| BshNI | GGYRCC | 1 cut(s) | 395 |
| BsiHKAI | GWGCWC | 2 cut(s) | 292, 372 |
| BsiSI | CCGG | 1 cut(s) | 447 |
| BslFI | GGGAC | 1 cut(s) | 457 |
| BsmAI | GTCTC | 1 cut(s) | 444 |
| BsmFI | GGGAC | 1 cut(s) | 457 |
| BsnI | GGCC | 2 cut(s) | 41, 188 |
| Bsp1286I | GDGCHC | 3 cut(s) | 292, 372, 380 |
| Bsp143I | GATC | 1 cut(s) | 424 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 203 |
| BspACI | CCGC | 2 cut(s) | 183, 185 |
| BspANI | GGCC | 2 cut(s) | 41, 188 |
| BspCNI | CTCAG | 1 cut(s) | 216 |
| BspFNI | CGCG | 1 cut(s) | 185 |
| BspLI | GGNNCC | 2 cut(s) | 397, 445 |
| BspPI | GGATC | 1 cut(s) | 432 |
| BspT107I | GGYRCC | 1 cut(s) | 395 |
| BssECI | CCNNGG | 2 cut(s) | 183, 279 |
| BssMI | GATC | 1 cut(s) | 424 |
| BssNI | GRCGYC | 2 cut(s) | 173, 396 |
| BstACI | GRCGYC | 2 cut(s) | 173, 396 |
| BstC8I | GCNNGC | 3 cut(s) | 196, 200, 224 |
| BstDEI | CTNAG | 1 cut(s) | 203 |
| BstDSI | CCRYGG | 2 cut(s) | 183, 279 |
| BstF5I | GGATG | 1 cut(s) | 117 |
| BstFNI | CGCG | 1 cut(s) | 185 |
| BstH2I | RGCGCY | 1 cut(s) | 399 |
| BstHHI | GCGC | 1 cut(s) | 398 |
| BstKTI | GATC | 1 cut(s) | 427 |
| BstMAI | GTCTC | 1 cut(s) | 444 |
| BstMBI | GATC | 1 cut(s) | 424 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 74, 191, 204, 338 |
| BstNSI | RCATGY | 1 cut(s) | 226 |
| BstSLI | GKGCMC | 1 cut(s) | 380 |
| BstUI | CGCG | 1 cut(s) | 185 |
| BstX2I | RGATCY | 1 cut(s) | 424 |
| BstYI | RGATCY | 1 cut(s) | 424 |
| BsuRI | GGCC | 2 cut(s) | 41, 188 |
| BtgI | CCRYGG | 2 cut(s) | 183, 279 |
| BtsCI | GGATG | 1 cut(s) | 117 |
| Cac8I | GCNNGC | 3 cut(s) | 196, 200, 224 |
| CfoI | GCGC | 1 cut(s) | 398 |
| Cfr13I | GGNCC | 2 cut(s) | 40, 444 |
| Cfr42I | CCGCGG | 1 cut(s) | 186 |
| Csp6I | GTAC | 1 cut(s) | 343 |
| CviAII | CATG | 3 cut(s) | 102, 223, 460 |
| CviQI | GTAC | 1 cut(s) | 343 |
| DdeI | CTNAG | 1 cut(s) | 203 |
| DinI | GGCGCC | 1 cut(s) | 397 |
| DpnI | GATC | 1 cut(s) | 426 |
| DpnII | GATC | 1 cut(s) | 424 |
| EaeI | YGGCCR | 1 cut(s) | 186 |
| Ecl136II | GAGCTC | 1 cut(s) | 290 |
| Eco24I | GRGCYC | 1 cut(s) | 292 |
| Eco47I | GGWCC | 1 cut(s) | 444 |
| Eco53kI | GAGCTC | 1 cut(s) | 290 |
| Eco57I | CTGAAG | 1 cut(s) | 542 |
| EcoICRI | GAGCTC | 1 cut(s) | 290 |
| EcoT38I | GRGCYC | 1 cut(s) | 292 |
| EgeI | GGCGCC | 1 cut(s) | 397 |
| EheI | GGCGCC | 1 cut(s) | 397 |
| FaeI | CATG | 3 cut(s) | 105, 226, 463 |
| FaiI | YATR | 6 cut(s) | 103, 224, 392, 461, 508, 543 |
| FaqI | GGGAC | 1 cut(s) | 457 |
| FatI | CATG | 3 cut(s) | 101, 222, 459 |
| Fnu4HI | GCNGC | 1 cut(s) | 186 |
| FokI | GGATG | 1 cut(s) | 124 |
| FriOI | GRGCYC | 1 cut(s) | 292 |
| Fsp4HI | GCNGC | 1 cut(s) | 186 |
| FspBI | CTAG | 2 cut(s) | 420, 465 |
| GlaI | GCGC | 1 cut(s) | 397 |
| GluI | GCNGC | 1 cut(s) | 186 |
| HaeII | RGCGCY | 1 cut(s) | 399 |
| HaeIII | GGCC | 2 cut(s) | 41, 188 |
| HapII | CCGG | 1 cut(s) | 447 |
| HhaI | GCGC | 1 cut(s) | 398 |
| Hin1I | GRCGYC | 2 cut(s) | 173, 396 |
| Hin1II | CATG | 3 cut(s) | 105, 226, 463 |
| Hin6I | GCGC | 1 cut(s) | 396 |
| HinP1I | GCGC | 1 cut(s) | 396 |
| HpaII | CCGG | 1 cut(s) | 447 |
| Hpy166II | GTNNAC | 1 cut(s) | 345 |
| Hpy188I | TCNGA | 2 cut(s) | 486, 522 |
| Hpy8I | GTNNAC | 1 cut(s) | 345 |
| Hpy99I | CGWCG | 3 cut(s) | 146, 175, 260 |
| HpyAV | CCTTC | 1 cut(s) | 148 |
| HpyCH4IV | ACGT | 2 cut(s) | 173, 380 |
| HpyCH4V | TGCA | 3 cut(s) | 105, 194, 499 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 74, 191, 204, 338 |
| HpyF3I | CTNAG | 1 cut(s) | 203 |
| HpySE526I | ACGT | 2 cut(s) | 173, 380 |
| Hsp92I | GRCGYC | 2 cut(s) | 173, 396 |
| Hsp92II | CATG | 3 cut(s) | 105, 226, 463 |
| HspAI | GCGC | 1 cut(s) | 396 |
| KasI | GGCGCC | 1 cut(s) | 395 |
| KspI | CCGCGG | 1 cut(s) | 186 |
| Kzo9I | GATC | 1 cut(s) | 424 |
| LpnPI | CCDG | 4 cut(s) | 85, 298, 460, 485 |
| MaeI | CTAG | 2 cut(s) | 420, 465 |
| MaeII | ACGT | 2 cut(s) | 173, 380 |
| MaeIII | GTNAC | 1 cut(s) | 178 |
| MalI | GATC | 1 cut(s) | 426 |
| MboI | GATC | 1 cut(s) | 424 |
| MboII | GAAGA | 2 cut(s) | 448, 535 |
| MflI | RGATCY | 1 cut(s) | 424 |
| MhlI | GDGCHC | 3 cut(s) | 292, 372, 380 |
| MluCI | AATT | 3 cut(s) | 83, 214, 471 |
| Mly113I | GGCGCC | 1 cut(s) | 396 |
| MmeI | TCCRAC | 1 cut(s) | 418 |
| MnlI | CCTC | 4 cut(s) | 53, 88, 434, 461 |
| MseI | TTAA | 1 cut(s) | 237 |
| MslI | CAYNNNNRTG | 1 cut(s) | 350 |
| MspA1I | CMGCKG | 1 cut(s) | 185 |
| MspI | CCGG | 1 cut(s) | 447 |
| MvnI | CGCG | 1 cut(s) | 185 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 74, 191, 204, 338 |
| NarI | GGCGCC | 1 cut(s) | 396 |
| NdeII | GATC | 1 cut(s) | 424 |
| NlaIII | CATG | 3 cut(s) | 105, 226, 463 |
| NlaIV | GGNNCC | 2 cut(s) | 397, 445 |
| NspI | RCATGY | 1 cut(s) | 226 |
| OliI | CACNNNNGTG | 1 cut(s) | 350 |
| PaeI | GCATGC | 1 cut(s) | 226 |
| PkrI | GCNGC | 1 cut(s) | 187 |
| PluTI | GGCGCC | 1 cut(s) | 399 |
| Psp124BI | GAGCTC | 1 cut(s) | 292 |
| PspN4I | GGNNCC | 2 cut(s) | 397, 445 |
| PspPI | GGNCC | 2 cut(s) | 40, 444 |
| PsuI | RGATCY | 1 cut(s) | 424 |
| RsaI | GTAC | 1 cut(s) | 344 |
| RsaNI | GTAC | 1 cut(s) | 343 |
| RseI | CAYNNNNRTG | 1 cut(s) | 350 |
| SacI | GAGCTC | 1 cut(s) | 292 |
| SacII | CCGCGG | 1 cut(s) | 186 |
| SaqAI | TTAA | 1 cut(s) | 237 |
| SatI | GCNGC | 1 cut(s) | 186 |
| Sau3AI | GATC | 1 cut(s) | 424 |
| Sau96I | GGNCC | 2 cut(s) | 40, 444 |
| SduI | GDGCHC | 3 cut(s) | 292, 372, 380 |
| SetI | ASST | 7 cut(s) | 29, 138, 176, 204, 292, 326, 383 |
| SfoI | GGCGCC | 1 cut(s) | 397 |
| Sfr303I | CCGCGG | 1 cut(s) | 186 |
| SgrBI | CCGCGG | 1 cut(s) | 186 |
| SinI | GGWCC | 1 cut(s) | 444 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 350 |
| SmlI | CTYRAG | 2 cut(s) | 291, 361 |
| SmoI | CTYRAG | 2 cut(s) | 291, 361 |
| SphI | GCATGC | 1 cut(s) | 226 |
| Sse9I | AATT | 3 cut(s) | 83, 214, 471 |
| SsiI | CCGC | 2 cut(s) | 183, 185 |
| SspDI | GGCGCC | 1 cut(s) | 395 |
| SspI | AATATT | 1 cut(s) | 388 |
| SspMI | CTAG | 2 cut(s) | 420, 465 |
| SstI | GAGCTC | 1 cut(s) | 292 |
| TaiI | ACGT | 2 cut(s) | 176, 383 |
| TaqI | TCGA | 2 cut(s) | 141, 255 |
| TasI | AATT | 3 cut(s) | 83, 214, 471 |
| TauI | GCSGC | 1 cut(s) | 188 |
| Tru1I | TTAA | 1 cut(s) | 237 |
| Tru9I | TTAA | 1 cut(s) | 237 |
| TspDTI | ATGAA | 3 cut(s) | 19, 257, 448 |
| TspGWI | ACGGA | 2 cut(s) | 164, 425 |
| VpaK11BI | GGWCC | 1 cut(s) | 444 |
| XapI | RAATTY | 1 cut(s) | 83 |
| XceI | RCATGY | 1 cut(s) | 226 |
| XspI | CTAG | 2 cut(s) | 420, 465 |
| ZraI | GACGTC | 1 cut(s) | 174 |