Rroxscaffold_3G00259960

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004981
Physical Location & Seq
Reverse (-)
54321344 .. 54321795
452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00259960.1

Sequence Viewer

Length: 363 bp
ATGCAAGAGGCTTGGGAGCACAAGTTTGGTATGCTGGTTGTCTTCAACGATCCTCTGACCAAAGACCACCATCTTCTGTCTCCTGCCGTCGGAAGAGCTCAAGGCTTCTATTTCTATGACATGAAGGACGACTACACTGCTTGGTTTGCCTACACTTTGGTGTTCAACTCGAGCGAGCACAAGGGTACACTCAATATCATGGGGGCGGATTTGATGTATGAAAAGACTAGGGATCTTTCGGTTGTCGGAGGGACGGGAGATTTCTTCATGGCTAGAGGGATTTGCACATTTCAAACTGATACTTTTCAGGGTGACTACTATTTTCGCCTCAAGATGGATATCAAGTTGTATGAATGTTATTAG

Protein Analysis

120

Amino Acids

14.03

Weight (kDa)

5.18

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 6 - 114 9.3e-33 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 206
AclWI GGATC 2 cut(s) 44, 240
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 89, 334
AgsI TTSAA 3 cut(s) 46, 166, 293
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 1 cut(s) 98
AluI AGCT 1 cut(s) 98
Alw21I GWGCWC 3 cut(s) 21, 100, 180
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 2 cut(s) 44, 240
Ama87I CYCGRG 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 169
BanII GRGCYC 1 cut(s) 100
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 3 cut(s) 21, 100, 180
BccI CCATC 2 cut(s) 78, 328
BceAI ACGGC 1 cut(s) 71
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 2 cut(s) 228, 273
BmeT110I CYCGRG 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 84, 314
BsaBI GATNNNNATC 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 334
Bse8I GATNNNNATC 1 cut(s) 338
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 2 cut(s) 89, 334
BsiHKAI GWGCWC 3 cut(s) 21, 100, 180
BsiHKCI CYCGRG 1 cut(s) 169
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 2 cut(s) 89, 334
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 265
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 3 cut(s) 21, 100, 180
Bsp143I GATC 2 cut(s) 49, 232
BspACI CCGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 44, 240
BspQI GCTCTTC 1 cut(s) 88
BssMI GATC 2 cut(s) 49, 232
Bst6I CTCTTC 1 cut(s) 88
BstC8I GCNNGC 1 cut(s) 176
BstKTI GATC 2 cut(s) 52, 235
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 49, 232
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstV2I GAAGAC 1 cut(s) 34
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 176
Csp6I GTAC 1 cut(s) 186
CviAII CATG 3 cut(s) 121, 199, 268
CviJI RGCY 4 cut(s) 11, 98, 105, 272
CviKI_1 RGCY 4 cut(s) 11, 98, 105, 272
CviQI GTAC 1 cut(s) 186
DpnI GATC 2 cut(s) 51, 234
DpnII GATC 2 cut(s) 49, 232
Eam1104I CTCTTC 1 cut(s) 88
EarI CTCTTC 1 cut(s) 88
EciI GGCGGA 1 cut(s) 221
Ecl136II GAGCTC 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 100
Eco32I GATATC 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 98
EcoRV GATATC 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 100
FaeI CATG 3 cut(s) 124, 202, 271
FaiI YATR 7 cut(s) 32, 117, 122, 200, 219, 269, 351
FaqI GGGAC 1 cut(s) 265
FatI CATG 3 cut(s) 120, 198, 267
FriOI GRGCYC 1 cut(s) 100
FspBI CTAG 2 cut(s) 228, 273
Hin1II CATG 3 cut(s) 124, 202, 271
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 3 cut(s) 57, 92, 248
Hpy188III TCNNGA 1 cut(s) 331
Hpy8I GTNNAC 1 cut(s) 188
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 118
HpyCH4V TGCA 2 cut(s) 4, 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
Hsp92II CATG 3 cut(s) 124, 202, 271
Kzo9I GATC 2 cut(s) 49, 232
LguI GCTCTTC 1 cut(s) 88
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 3 cut(s) 20, 96, 293
MaeI CTAG 2 cut(s) 228, 273
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 51, 234
MboI GATC 2 cut(s) 49, 232
MboII GAAGA 4 cut(s) 34, 65, 105, 256
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 3 cut(s) 21, 100, 180
MmeI TCCRAC 2 cut(s) 70, 226
MnlI CCTC 4 cut(s) 63, 242, 269, 338
MslI CAYNNNNRTG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 2 cut(s) 49, 232
NlaIII CATG 3 cut(s) 124, 202, 271
NmuCI GTSAC 1 cut(s) 311
OliI CACNNNNGTG 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 88
Psp124BI GAGCTC 1 cut(s) 100
PspXI VCTCGAGB 1 cut(s) 169
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 88
Sau3AI GATC 2 cut(s) 49, 232
SduI GDGCHC 3 cut(s) 21, 100, 180
SetI ASST 1 cut(s) 100
Sfr274I CTCGAG 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 3 cut(s) 99, 169, 329
SmoI CTYRAG 3 cut(s) 99, 169, 329
SsiI CCGC 1 cut(s) 206
SspMI CTAG 2 cut(s) 228, 273
SstI GAGCTC 1 cut(s) 100
TaqI TCGA 1 cut(s) 170
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 3 cut(s) 137, 234, 256
TspRI CASTG 1 cut(s) 142
XhoI CTCGAG 1 cut(s) 169
XspI CTAG 2 cut(s) 228, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.