RchiOBHm_Chr7g0196001

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004981
Physical Location & Seq
Forward (+)
13850114 .. 13850656
543 bp
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UTR
Exon/CDS
Intron
PRQ17529

Sequence Viewer

Length: 543 bp
ATGGCAACTTTTGTAGAAAAAATTTGTTTCATCATTTTGATCCTCATCGCATTCCAATCTGTTTCGGCAATGAAACATTCAATCATAGAGTCGAAACCATGCAAGAGGCTCGTGCTCTATTACCACGACATCCTTTTCAATGGCACTGATGTGGCAAATGCTACATCTGCTAGGGCAGCAAATGGGACGGGCCTCGGCAACTACCAGTTTGGTATGCTGGTTGTCTTCAATGATCCTCTGACCAAAGACCACCATCTTCTGTCTCCTGCCGTCGGAAGAGCTCAAGGCTTCTTTTTCTATGACATGAAGGACGACTACACTGCCTGGTTTGCCTACACTTTGGTGTTCAACTCGAGCGAGCACAAGGGTACACTCAATATCATGGGGGCGGATTTGATGTATGCCGAGACTAGGGATATTTCGGTGGTTGGAGGAACGGGAGATTTCTTCATGGCTAGAGGGATTTGCACATTCAAGACTGATACTTTTCAGGGTGCCAACTATTTTCGTCTCCAAATGGACATCAAGTTGTATGAATGTTAG

Protein Analysis

180

Amino Acids

20.22

Weight (kDa)

6.17

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 34 - 176 3.2e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AciI CCGC 1 cut(s) 389
AclWI GGATC 2 cut(s) 34, 227
AcsI RAATTY 1 cut(s) 21
AfaI GTAC 1 cut(s) 370
AfiI CCNNNNNNNGG 2 cut(s) 272, 411
AgsI TTSAA 5 cut(s) 81, 139, 229, 349, 475
AjnI CCWGG 1 cut(s) 323
AleI CACNNNNGTG 2 cut(s) 149, 341
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
Alw21I GWGCWC 3 cut(s) 117, 283, 363
Alw26I GTCTC 3 cut(s) 267, 401, 515
AlwI GGATC 2 cut(s) 34, 227
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 190
AvaI CYCGRG 1 cut(s) 352
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 283
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BauI CACGAG 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 3 cut(s) 117, 283, 363
BbvI GCAGC 1 cut(s) 188
BccI CCATC 1 cut(s) 261
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 91, 125, 302, 336
BciT130I CCWGG 1 cut(s) 325
BcoDI GTCTC 3 cut(s) 267, 401, 515
BfaI CTAG 3 cut(s) 171, 411, 456
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 217
BpuEI CTTGAG 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 411
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 1 cut(s) 75
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 193
BseGI GGATG 1 cut(s) 129
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 2 cut(s) 272, 411
BseMI GCAATG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 1 cut(s) 188
BshFI GGCC 1 cut(s) 192
BshNI GGYRCC 1 cut(s) 494
BsiHKAI GWGCWC 3 cut(s) 117, 283, 363
BsiHKCI CYCGRG 1 cut(s) 352
BslFI GGGAC 1 cut(s) 199
BslI CCNNNNNNNGG 2 cut(s) 272, 411
BsmAI GTCTC 3 cut(s) 267, 401, 515
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 1 cut(s) 192
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 117, 283, 363
Bsp143I GATC 2 cut(s) 39, 232
BspACI CCGC 1 cut(s) 389
BspANI GGCC 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 496
BspPI GGATC 2 cut(s) 34, 227
BspQI GCTCTTC 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 75
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 2 cut(s) 39, 232
BssSI CACGAG 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 359
BstF5I GGATG 1 cut(s) 129
BstKTI GATC 2 cut(s) 42, 235
BstMAI GTCTC 3 cut(s) 267, 401, 515
BstMBI GATC 2 cut(s) 39, 232
BstMWI GCNNNNNNNGC 3 cut(s) 167, 176, 329
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 217
BsuRI GGCC 1 cut(s) 192
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 2 cut(s) 144, 318
Cac8I GCNNGC 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 369
CviAII CATG 4 cut(s) 99, 304, 382, 451
CviJI RGCY 5 cut(s) 109, 192, 281, 288, 455
CviKI_1 RGCY 5 cut(s) 109, 192, 281, 288, 455
CviQI GTAC 1 cut(s) 369
DpnI GATC 2 cut(s) 41, 234
DpnII GATC 2 cut(s) 39, 232
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
EciI GGCGGA 1 cut(s) 404
Ecl136II GAGCTC 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 515
FaeI CATG 4 cut(s) 102, 307, 385, 454
FaiI YATR 9 cut(s) 86, 100, 215, 300, 305, 383, 402, 452, 534
FaqI GGGAC 1 cut(s) 199
FatI CATG 4 cut(s) 98, 303, 381, 450
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 3 cut(s) 171, 411, 456
GluI GCNGC 1 cut(s) 177
HaeIII GGCC 1 cut(s) 192
Hin1II CATG 4 cut(s) 102, 307, 385, 454
HinfI GANTC 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 371
Hpy188I TCNGA 2 cut(s) 240, 275
Hpy188III TCNNGA 1 cut(s) 475
Hpy8I GTNNAC 1 cut(s) 371
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 1 cut(s) 301
HpyCH4V TGCA 2 cut(s) 102, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 176, 329
Hsp92II CATG 4 cut(s) 102, 307, 385, 454
Kzo9I GATC 2 cut(s) 39, 232
LguI GCTCTTC 1 cut(s) 271
LpnPI CCDG 6 cut(s) 203, 218, 279, 310, 337, 476
Lsp1109I GCAGC 1 cut(s) 188
MaeI CTAG 3 cut(s) 171, 411, 456
MalI GATC 2 cut(s) 41, 234
MboI GATC 2 cut(s) 39, 232
MboII GAAGA 4 cut(s) 217, 248, 288, 439
MhlI GDGCHC 3 cut(s) 117, 283, 363
MluCI AATT 1 cut(s) 21
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 253, 409
MnlI CCTC 6 cut(s) 53, 99, 203, 246, 425, 452
MslI CAYNNNNRTG 2 cut(s) 149, 341
MspR9I CCNGG 1 cut(s) 325
Mva1269I GAATGC 1 cut(s) 50
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 3 cut(s) 167, 176, 329
NdeII GATC 2 cut(s) 39, 232
NlaIII CATG 4 cut(s) 102, 307, 385, 454
NlaIV GGNNCC 1 cut(s) 496
NmeAIII GCCGAG 2 cut(s) 174, 430
OliI CACNNNNGTG 2 cut(s) 149, 341
PaeR7I CTCGAG 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 271
PctI GAATGC 1 cut(s) 50
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 1 cut(s) 190
PspXI VCTCGAGB 1 cut(s) 352
RsaI GTAC 1 cut(s) 370
RsaNI GTAC 1 cut(s) 369
RseI CAYNNNNRTG 2 cut(s) 149, 341
SacI GAGCTC 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 271
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 39, 232
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 3 cut(s) 117, 283, 363
SetI ASST 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 149, 341
SmlI CTYRAG 2 cut(s) 282, 352
SmoI CTYRAG 2 cut(s) 282, 352
Sse9I AATT 1 cut(s) 21
SsiI CCGC 1 cut(s) 389
SspMI CTAG 3 cut(s) 171, 411, 456
SstI GAGCTC 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 323
TaqI TCGA 2 cut(s) 92, 353
TasI AATT 1 cut(s) 21
TscAI CASTG 2 cut(s) 151, 325
TseI GCWGC 1 cut(s) 176
TspDTI ATGAA 4 cut(s) 19, 86, 320, 439
TspRI CASTG 2 cut(s) 151, 325
XapI RAATTY 1 cut(s) 21
XhoI CTCGAG 1 cut(s) 352
XspI CTAG 3 cut(s) 171, 411, 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.