Prupe.6G111100_v2.0.a1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0002027
Physical Location & Seq
Forward (+)
7978664 .. 7980630
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G111100.1

Sequence Viewer

Length: 1704 bp
ATGAGCAGTTCTATCAATAGCAGCAGTAGCAGCAGAAGCAGTTGTGGATTGTGTCCATTTGCAGTGCAGGTGATTAAGGGAAGATGGTTCACAGTTTTTGCCTCTTTTCTCATCATGGCTGGAGCTGGTGCAACTTATCTCTTTGGTGTGTATTCAAAAGACATCAAGTCCACTCTTGGATATGACCAAACCACCCTCAACTTGTTAGGCTTCTTCAAAGATCTTGGTGCAAATGTTGGTGTGCTCTCAGGCCTAATTGGTGAGGTAACTCCAGCTTGGTTTGTGCTCCTTGTAGGTTCTGCTATGAATTTTGCAGGCTACTTCATGATTTGGCTTTCTGTGACTGAGAAAATTGCCAAGCCTAAAGTTTGGCAGATGTGTCTTTACATATGCCTTGGAGCCAATTCTCAGAATTTTGCAAATACAGGAGCTCTTGTCACTTGTGTCAAGAATTTCCCAGAGAGTAGAGGTGTTATGCTTGGTCTGTTAAAGGGTTTTACTGGGCTCAGTGGAGCTATTTTTACCCAACTTTACTTGGCAATTTACCCGAATGACTCAAAATCTTTGATTCTTCTAATTGGGTGGCTGCCTGCTGCACTATCAGTTGTTTTTGTTTACACCATTAGACCAATGAAAGCTGTTAAGCAACAAAATGAGCTGAGAGTCTTTTATCACTTCCTCTATATCTCAGTCTCCCTTGCATTGTTCATCCTGGCTATGAGTCTCATTCAAAAACAGGTTGCTTTCTCCCAAGCTGCCTATGCCGGAACTTCCGCTGTCGTTTGTCTGTTCCTCTTTCTCCCTCTTGCCATTGCAATTAGAGAAGAGCTAGTCCTCTGGAACCTCAAGAAAAAACCAGTTGATCCTCCAACTGAGGTGTCCATTGAGAAACCACAAGAAATTCAAGAAATAAAGGATGATTCAGATTTGCAAGTACAGAACACAAAGAGCACAACTTGTTTTGGCGACATTTGCAATAAACCAGAAAGAGGAGAAGACTATACAATTCTACAAGCACTCCTGAGCATGGACATGATGATATTGTTCATTGCAACATTTTGTGGACTTGGGTCAAGTTTGACAGCAGTAGACAACTTGGGTCAAATTGGTGAGTCTCTGGGATATCCAACCAAAACAATAAAAACATTTGTGTCCTTGGTGAGCATTTGGAACTACTTTGGGAGGGTCTTTTCTGGGTTTGTCTCTGAAAACCTAATCGTAAAGTGGAAGGTTCCAAGAACACTCATGATGACCTTTATGCTTGTTCTTTCATCTCTTGCCTACCTCTCCATTGCCTTCCCATTCCCTGGTTCAGTCTATGTGGCATCAGTGATCATTGGAGTTTCTTTTGGTGCTCAATTGACATTGCTTTTCACAATCATTTCTGAGCTGTTTGGCCTAAAGTACTTTGCCACATTGTTTAATTGTGGACAACTAGCAAGCCCACTTGGGTCTTATATTCTGAATGTAAAGGTCACTGGGATGCTCTATGACAGGGAGGCATTGAAACAGCTAGCAGAGAAAGGGTTGACTAGGTCATCAGTGAAGGAGCTGACTTGCCTTGGGGGTCAATGCTATAGGCTTTCTTTCTCCATTTTAGCTGCTGTCACATTCTTTGGTGCTCTTGTTTCTCTGGTTTTGGTGATGAGGACAAGAAAGTTCTATAAAGGTGATATATATAAGAAGTATAGAGAGGCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

568

Amino Acids

62.34

Weight (kDa)

9.15

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 58
Acc36I ACCTGC 1 cut(s) 58
AccB7I CCANNNNNTGG 1 cut(s) 1307
AccI GTMKAC 1 cut(s) 1089
AciI CCGC 1 cut(s) 774
AclWI GGATC 1 cut(s) 857
AcsI RAATTY 4 cut(s) 307, 412, 451, 900
AfaI GTAC 2 cut(s) 936, 1406
AfiI CCNNNNNNNGG 3 cut(s) 989, 1027, 1307
AgsI TTSAA 5 cut(s) 156, 217, 731, 905, 1507
AhdI GACNNNNNGTC 1 cut(s) 166
AjnI CCWGG 2 cut(s) 711, 1306
Alw21I GWGCWC 6 cut(s) 246, 288, 433, 953, 1357, 1624
Alw26I GTCTC 4 cut(s) 697, 728, 1119, 1207
AlwI GGATC 1 cut(s) 857
AoxI GGCC 2 cut(s) 250, 1396
ApeKI GCWGC 7 cut(s) 21, 30, 586, 593, 755, 1601, 1697
ApoI RAATTY 4 cut(s) 307, 412, 451, 900
AsuHPI GGTGA 6 cut(s) 82, 272, 1121, 1171, 1654, 1682
AsuNHI GCTAGC 1 cut(s) 1513
BanII GRGCYC 2 cut(s) 433, 507
BbsI GAAGAC 1 cut(s) 1002
Bbv12I GWGCWC 6 cut(s) 246, 288, 433, 953, 1357, 1624
BbvI GCAGC 7 cut(s) 33, 42, 573, 580, 742, 1588, 1684
BccI CCATC 1 cut(s) 78
BciT130I CCWGG 2 cut(s) 713, 1308
BclI TGATCA 1 cut(s) 1332
BcoDI GTCTC 4 cut(s) 697, 728, 1119, 1207
BfaI CTAG 4 cut(s) 830, 1436, 1514, 1533
BfmI CTRYAG 1 cut(s) 1576
BfuAI ACCTGC 1 cut(s) 58
BglII AGATCT 1 cut(s) 220
BisI GCNGC 7 cut(s) 22, 31, 587, 594, 756, 1602, 1698
BlsI GCNGC 7 cut(s) 23, 32, 588, 595, 757, 1603, 1699
BmcAI AGTACT 1 cut(s) 1406
Bme1390I CCNGG 2 cut(s) 713, 1308
BmeRI GACNNNNNGTC 1 cut(s) 166
BmiI GGNNCC 3 cut(s) 400, 842, 1233
BmrFI CCNGG 2 cut(s) 713, 1308
BmrI ACTGGG 2 cut(s) 510, 1488
BmsI GCATC 2 cut(s) 1334, 1473
BmtI GCTAGC 1 cut(s) 1517
BmuI ACTGGG 2 cut(s) 510, 1488
BoxI GACNNNNGTC 1 cut(s) 1069
BpiI GAAGAC 1 cut(s) 1002
BpmI CTGGAG 2 cut(s) 141, 255
Bpu10I CCTNAGC 1 cut(s) 1022
BpuEI CTTGAG 1 cut(s) 830
BsaJI CCNNGG 4 cut(s) 394, 1155, 1306, 1561
BsaXI ACNNNNNCTCC 4 cut(s) 420, 450, 1002, 1032
Bsc4I CCNNNNNNNGG 3 cut(s) 989, 1027, 1307
Bse1I ACTGG 3 cut(s) 505, 857, 1483
Bse3DI GCAATG 4 cut(s) 810, 1047, 1290, 1364
BseBI CCWGG 2 cut(s) 713, 1308
BseDI CCNNGG 4 cut(s) 394, 1155, 1306, 1561
BseGI GGATG 3 cut(s) 708, 922, 1488
BseLI CCNNNNNNNGG 3 cut(s) 989, 1027, 1307
BseMI GCAATG 4 cut(s) 810, 1047, 1290, 1364
BseMII CTCAG 9 cut(s) 261, 336, 422, 520, 650, 702, 864, 1013, 1377
BseNI ACTGG 3 cut(s) 505, 857, 1483
BseRI GAGGAG 1 cut(s) 1005
BseXI GCAGC 7 cut(s) 33, 42, 573, 580, 742, 1588, 1684
BsgI GTGCAG 2 cut(s) 86, 579
BshFI GGCC 2 cut(s) 252, 1398
BsiHKAI GWGCWC 6 cut(s) 246, 288, 433, 953, 1357, 1624
BsiSI CCGG 1 cut(s) 765
BslI CCNNNNNNNGG 3 cut(s) 989, 1027, 1307
BsmAI GTCTC 4 cut(s) 697, 728, 1119, 1207
BsnI GGCC 2 cut(s) 252, 1398
Bsp1286I GDGCHC 7 cut(s) 246, 288, 433, 507, 953, 1357, 1624
Bsp143I GATC 3 cut(s) 220, 862, 1332
BspACI CCGC 1 cut(s) 774
BspANI GGCC 2 cut(s) 252, 1398
BspCNI CTCAG 9 cut(s) 260, 337, 421, 519, 651, 701, 865, 1014, 1378
BspHI TCATGA 2 cut(s) 324, 1245
BspLI GGNNCC 3 cut(s) 400, 842, 1233
BspMI ACCTGC 1 cut(s) 58
BspOI GCTAGC 1 cut(s) 1517
BspPI GGATC 1 cut(s) 857
BspQI GCTCTTC 1 cut(s) 819
BsrDI GCAATG 4 cut(s) 810, 1047, 1290, 1364
BsrI ACTGG 3 cut(s) 505, 857, 1483
BssECI CCNNGG 4 cut(s) 394, 1155, 1306, 1561
BssMI GATC 3 cut(s) 220, 862, 1332
BssT1I CCWWGG 3 cut(s) 394, 1155, 1561
Bst2UI CCWGG 2 cut(s) 713, 1308
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 819
BstC8I GCNNGC 4 cut(s) 316, 591, 1441, 1515
BstDEI CTNAG 9 cut(s) 247, 345, 408, 506, 659, 688, 873, 1022, 1386
BstF5I GGATG 3 cut(s) 708, 922, 1488
BstKTI GATC 3 cut(s) 223, 865, 1335
BstMAI GTCTC 4 cut(s) 697, 728, 1119, 1207
BstMBI GATC 3 cut(s) 220, 862, 1332
BstMWI GCNNNNNNNGC 5 cut(s) 27, 30, 36, 761, 972
BstNI CCWGG 2 cut(s) 713, 1308
BstPAI GACNNNNGTC 1 cut(s) 1069
BstSCI CCNGG 2 cut(s) 711, 1306
BstSFI CTRYAG 1 cut(s) 1576
BstV1I GCAGC 7 cut(s) 33, 42, 573, 580, 742, 1588, 1684
BstV2I GAAGAC 1 cut(s) 1002
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 2 cut(s) 252, 1398
BtsCI GGATG 3 cut(s) 708, 922, 1488
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 5 cut(s) 69, 514, 1335, 1476, 1548
BveI ACCTGC 1 cut(s) 58
Cac8I GCNNGC 4 cut(s) 316, 591, 1441, 1515
CciI TCATGA 2 cut(s) 324, 1245
Csp6I GTAC 2 cut(s) 935, 1405
CviAII CATG 5 cut(s) 115, 325, 1027, 1033, 1246
CviQI GTAC 2 cut(s) 935, 1405
DdeI CTNAG 9 cut(s) 247, 345, 408, 506, 659, 688, 873, 1022, 1386
DpnI GATC 3 cut(s) 222, 864, 1334
DpnII GATC 3 cut(s) 220, 862, 1332
DriI GACNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 819
Eam1105I GACNNNNNGTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 819
Ecl136II GAGCTC 1 cut(s) 431
Eco130I CCWWGG 3 cut(s) 394, 1155, 1561
Eco147I AGGCCT 1 cut(s) 252
Eco24I GRGCYC 2 cut(s) 433, 507
Eco32I GATATC 1 cut(s) 1124
Eco53kI GAGCTC 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 711, 1306
EcoRV GATATC 1 cut(s) 1124
EcoT14I CCWWGG 3 cut(s) 394, 1155, 1561
EcoT38I GRGCYC 2 cut(s) 433, 507
ErhI CCWWGG 3 cut(s) 394, 1155, 1561
FaeI CATG 5 cut(s) 118, 328, 1030, 1036, 1249
FatI CATG 5 cut(s) 114, 324, 1026, 1032, 1245
FauNDI CATATG 1 cut(s) 389
FbaI TGATCA 1 cut(s) 1332
FblI GTMKAC 1 cut(s) 1089
Fnu4HI GCNGC 7 cut(s) 22, 31, 587, 594, 756, 1602, 1698
FokI GGATG 3 cut(s) 695, 929, 1495
FriOI GRGCYC 2 cut(s) 433, 507
Fsp4HI GCNGC 7 cut(s) 22, 31, 587, 594, 756, 1602, 1698
FspBI CTAG 4 cut(s) 830, 1436, 1514, 1533
GluI GCNGC 7 cut(s) 22, 31, 587, 594, 756, 1602, 1698
GsuI CTGGAG 2 cut(s) 141, 255
HaeIII GGCC 2 cut(s) 252, 1398
HapII CCGG 1 cut(s) 765
Hin1II CATG 5 cut(s) 118, 328, 1030, 1036, 1249
HincII GTYRAC 1 cut(s) 1530
HindII GTYRAC 1 cut(s) 1530
HinfI GANTC 6 cut(s) 554, 568, 663, 721, 920, 1112
HpaII CCGG 1 cut(s) 765
HphI GGTGA 6 cut(s) 82, 272, 1121, 1171, 1654, 1682
Hpy166II GTNNAC 7 cut(s) 90, 171, 616, 1064, 1090, 1430, 1530
Hpy188I TCNGA 5 cut(s) 411, 925, 1207, 1387, 1464
Hpy188III TCNNGA 7 cut(s) 325, 448, 838, 847, 905, 1021, 1246
Hpy8I GTNNAC 7 cut(s) 90, 171, 616, 1064, 1090, 1430, 1530
HpyAV CCTTC 3 cut(s) 1222, 1306, 1540
HpyCH4III ACNGT 1 cut(s) 94
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 30, 36, 761, 972
HpyF3I CTNAG 9 cut(s) 247, 345, 408, 506, 659, 688, 873, 1022, 1386
Hsp92II CATG 5 cut(s) 118, 328, 1030, 1036, 1249
Ksp22I TGATCA 1 cut(s) 1332
Kzo9I GATC 3 cut(s) 220, 862, 1332
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 6 cut(s) 122, 291, 398, 428, 512, 1549
Lsp1109I GCAGC 7 cut(s) 33, 42, 573, 580, 742, 1588, 1684
LweI GCATC 2 cut(s) 1334, 1473
MaeI CTAG 4 cut(s) 830, 1436, 1514, 1533
MaeIII GTNAC 5 cut(s) 265, 340, 436, 1474, 1606
MalI GATC 3 cut(s) 222, 864, 1334
MboI GATC 3 cut(s) 220, 862, 1332
MboII GAAGA 5 cut(s) 93, 205, 563, 836, 1007
MfeI CAATTG 1 cut(s) 1358
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 7 cut(s) 246, 288, 433, 507, 953, 1357, 1624
MlyI GAGTC 4 cut(s) 548, 672, 730, 1121
MmeI TCCRAC 2 cut(s) 893, 1151
MseI TTAA 4 cut(s) 75, 488, 642, 1422
MslI CAYNNNNRTG 2 cut(s) 1031, 1481
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 2 cut(s) 713, 1308
MunI CAATTG 1 cut(s) 1358
MvaI CCWGG 2 cut(s) 713, 1308
MwoI GCNNNNNNNGC 5 cut(s) 27, 30, 36, 761, 972
NdeI CATATG 1 cut(s) 389
NdeII GATC 3 cut(s) 220, 862, 1332
NheI GCTAGC 1 cut(s) 1513
NlaIII CATG 5 cut(s) 118, 328, 1030, 1036, 1249
NlaIV GGNNCC 3 cut(s) 400, 842, 1233
NmuCI GTSAC 4 cut(s) 340, 436, 1474, 1606
PagI TCATGA 2 cut(s) 324, 1245
PaqCI CACCTGC 1 cut(s) 58
PceI AGGCCT 1 cut(s) 252
PciSI GCTCTTC 1 cut(s) 819
PfeI GAWTC 2 cut(s) 568, 920
PflFI GACNNNGTC 1 cut(s) 1534
PflMI CCANNNNNTGG 1 cut(s) 1307
PkrI GCNGC 7 cut(s) 23, 32, 588, 595, 757, 1603, 1699
PleI GAGTC 4 cut(s) 548, 671, 729, 1120
PpsI GAGTC 4 cut(s) 548, 671, 729, 1120
PshAI GACNNNNGTC 1 cut(s) 1069
Psp124BI GAGCTC 1 cut(s) 433
Psp6I CCWGG 2 cut(s) 711, 1306
PspGI CCWGG 2 cut(s) 711, 1306
PspN4I GGNNCC 3 cut(s) 400, 842, 1233
PsuI RGATCY 1 cut(s) 220
PsyI GACNNNGTC 1 cut(s) 1534
RsaI GTAC 2 cut(s) 936, 1406
RsaNI GTAC 2 cut(s) 935, 1405
RseI CAYNNNNRTG 2 cut(s) 1031, 1481
SacI GAGCTC 1 cut(s) 433
SapI GCTCTTC 1 cut(s) 819
SaqAI TTAA 4 cut(s) 75, 488, 642, 1422
SatI GCNGC 7 cut(s) 22, 31, 587, 594, 756, 1602, 1698
Sau3AI GATC 3 cut(s) 220, 862, 1332
ScaI AGTACT 1 cut(s) 1406
SchI GAGTC 4 cut(s) 548, 672, 730, 1121
ScrFI CCNGG 2 cut(s) 713, 1308
SduI GDGCHC 7 cut(s) 246, 288, 433, 507, 953, 1357, 1624
SfaNI GCATC 2 cut(s) 1334, 1473
SfcI CTRYAG 1 cut(s) 1576
SmiMI CAYNNNNRTG 2 cut(s) 1031, 1481
SmlI CTYRAG 1 cut(s) 845
SmoI CTYRAG 1 cut(s) 845
SseBI AGGCCT 1 cut(s) 252
SsiI CCGC 1 cut(s) 774
SspMI CTAG 4 cut(s) 830, 1436, 1514, 1533
SstI GAGCTC 1 cut(s) 433
StuI AGGCCT 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 711, 1306
StyI CCWWGG 3 cut(s) 394, 1155, 1561
TaaI ACNGT 1 cut(s) 94
TatI WGTACW 2 cut(s) 934, 1404
TfiI GAWTC 2 cut(s) 568, 920
Tru1I TTAA 4 cut(s) 75, 488, 642, 1422
Tru9I TTAA 4 cut(s) 75, 488, 642, 1422
TscAI CASTG 5 cut(s) 69, 514, 1335, 1483, 1548
TseFI GTSAC 4 cut(s) 340, 436, 1474, 1606
TseI GCWGC 7 cut(s) 21, 30, 586, 593, 755, 1601, 1697
Tsp45I GTSAC 4 cut(s) 340, 436, 1474, 1606
TspDTI ATGAA 6 cut(s) 313, 320, 647, 697, 1036, 1260
TspRI CASTG 5 cut(s) 69, 514, 1335, 1483, 1548
Tth111I GACNNNGTC 1 cut(s) 1534
Van91I CCANNNNNTGG 1 cut(s) 1307
XapI RAATTY 4 cut(s) 307, 412, 451, 900
XmiI GTMKAC 1 cut(s) 1089
XspI CTAG 4 cut(s) 830, 1436, 1514, 1533
ZrmI AGTACT 1 cut(s) 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.