Rh5CG408900

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002027
Physical Location & Seq
Reverse (-)
54879939 .. 54882233
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG408900.1

Sequence Viewer

Length: 1707 bp
ATGGCATGCCAATTTGCAGTGCAGGTGATCAAAGGAAGATGGTTCACCGTGTTTGCCTCGTTTCTGATCATGGCTGGAGCCGGTGCTACTTATCTGTTTGGTTCTTATTCCAAAGACATCAAGTCCACTCTTGGCTATGATCAATCAACTCTCAACTTGATTGGCTTCTTCAAGGACCTTGGTGCAAATGTGGGAGTCCTCGCAGGTCTAATAGGTGAAGTAACTCCAACTTGGTTTGTTCTTCTAGTTGGTTCTGCCATGAATTTTGCAGGGTATTTCATGATTTGGCTTGCTGTCACTGGCAAAATAGCCAAACCTAAAGTCTGGCAGATGTGCCTTTACATCTGCGTTGGGGCCAATTCTCAGAATTTTGTAAACACAGGTGCCCTTGTCACTTGTGTCAAGAATTTCCCAGAAAGTAGAGGTGTTATGCTTGGTTTGTTAAAGGGTTATACTGGGCTTAGTGGAGCAATATTCACCCAAATTTACTTGGCTATTTACCCAAACGATTCAAAGTCTTTGATTCTTTTGATTGGTTGGCTTCCTGCTGCTCTTTCAGTCATTTTTGTATACACCATTCGTCCAATGAAAGTTGTTAGGCAACAAAATGAGCTGAGAGTCTTTTACCATTTCCTCTATATCTCACTTTCTCTTGGAGTTTTCCTCATGGTAATGACTCTGCTTGAAAAAACAATCACTTTCTCTCGTGCTGCTTATGCCGGAAGCACCACCGTGGTTTGTATGATCATGTTCATGCCTCTTGGAATTGCAATCAGAGAAGAGCTTGCACTGTGGAATCTCAAGAAACATCCTGTTGATTCTCCAGTTGAGGTGATAAGCATTGATAAAAGACCAAACCCAGTTCCAGAAATTCAAGAAATTCAAGAACTGGATTCTTTCCCACCCAAATCCTCATTAGGCCAGGAAAAGAAAACACAGATTACTACTACCGCTACATCTTGCTGTGGTGATCTATGCAATAAGCCAGAAAGAGGGGAAGACTATACAATACTACAAGCACTTTTGAGTACGGATATGATTATTCTATTCATTGCAACTTTTTGTGGGTTGGGGTCAAGTTTGACAGCAGTAGACAATTTGGGTCAGATTGGTGAGTCTCTGGGATATCCCACTAAGACAGTAGGCACTTTTGTGTCATTAGTGAGCATTTGGAATTATTTTGGGAGGGTCTTTTCTGGTTTTGTCTCTGAGAGCCTAATGGTGAAGTGGAAAATCCCCAGAACACTCATGATGACCTTTATGCTTGTTCTATCATGTATTGCATATCTCTCCATTGCTTTCCCATTCCCTGGTTCAGTTTATATGGCTTCGGTTATGATTGGGGTTTCTTTTGGTGCTCAATTGACACTGCTTTTCACAATCATATCTGAGCTGTTTGGGCTCAAGTACTATGCTACATTGTTTAACTGCGGACAGCTAGCGAGTCCTCTCGGGTCTTACATTCTCAATGTGAGGGTCACCGGGATGCTCTATGATCGAGAAGCTTTGAAGGTGTTGGCAAAGAAAGGGATGACAAGATCTTCAGTGAAGGAGCTGGTTTGCATTGGAAGTCAGTGTTATAGGCTGTCTTTTACAATTTTGGCCGGTGTGACATTCTTTGGTGCTCTTATTTCACTGGTTTTGGTGATTAGGACTAGAGAGTTCTACAAAGGTGATATTTACAAGAAGTATAGAGAAGTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

568

Amino Acids

62.7

Weight (kDa)

9.13

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 13 - 257 1e-94 Nodulin-like
MFS_1 PF07690 338 - 475 3.6e-06 Major Facilitator Superfamily
NFD4_C PF23262 345 - 556 5.6e-15 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 2 cut(s) 13, 194
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 1310
AccI GTMKAC 2 cut(s) 570, 1092
AciI CCGC 2 cut(s) 951, 1431
AcoI YGGCCR 1 cut(s) 1602
AcsI RAATTY 6 cut(s) 262, 367, 406, 483, 870, 879
AcuI CTGAAG 1 cut(s) 1527
AfaI GTAC 2 cut(s) 1030, 1409
AfiI CCNNNNNNNGG 2 cut(s) 992, 1310
AgsI TTSAA 6 cut(s) 172, 513, 686, 875, 884, 1510
AhdI GACNNNNNGTC 1 cut(s) 121
AjnI CCWGG 2 cut(s) 921, 1309
AleI CACNNNNGTG 2 cut(s) 731, 1151
AluBI AGCT 6 cut(s) 613, 784, 1393, 1438, 1505, 1555
AluI AGCT 6 cut(s) 613, 784, 1393, 1438, 1505, 1555
Alw21I GWGCWC 2 cut(s) 1360, 1627
Alw26I GTCTC 2 cut(s) 1122, 1210
Ama87I CYCGRG 1 cut(s) 1451
AoxI GGCC 3 cut(s) 354, 919, 1602
ApeKI GCWGC 2 cut(s) 548, 710
ApoI RAATTY 6 cut(s) 262, 367, 406, 483, 870, 879
ArsI GACNNNNNNTTYG 2 cut(s) 306, 338
AspS9I GGNCC 2 cut(s) 175, 354
AsuC2I CCSGG 1 cut(s) 1483
AsuNHI GCTAGC 1 cut(s) 1438
AvaI CYCGRG 1 cut(s) 1451
AvaII GGWCC 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 1404
BauI CACGAG 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 2 cut(s) 1360, 1627
BbvI GCAGC 2 cut(s) 535, 697
BccI CCATC 1 cut(s) 33
BciT130I CCWGG 2 cut(s) 923, 1311
BclI TGATCA 4 cut(s) 27, 66, 139, 744
BcnI CCSGG 1 cut(s) 1483
BcoDI GTCTC 2 cut(s) 1122, 1210
BfaI CTAG 3 cut(s) 245, 1439, 1656
BfuAI ACCTGC 2 cut(s) 13, 194
BglII AGATCT 1 cut(s) 1538
BisI GCNGC 2 cut(s) 549, 711
BlsI GCNGC 2 cut(s) 550, 712
BmcAI AGTACT 1 cut(s) 1409
Bme1390I CCNGG 3 cut(s) 923, 1311, 1483
Bme18I GGWCC 1 cut(s) 175
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 1451
BmgT120I GGNCC 2 cut(s) 175, 354
BmiI GGNNCC 3 cut(s) 79, 355, 385
BmrFI CCNGG 3 cut(s) 923, 1311, 1483
BmrI ACTGGG 2 cut(s) 465, 854
BmsI GCATC 1 cut(s) 1476
BmtI GCTAGC 1 cut(s) 1442
BmuI ACTGGG 2 cut(s) 465, 854
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 648, 680
BpmI CTGGAG 2 cut(s) 96, 807
BpuEI CTTGAG 2 cut(s) 785, 1388
BpuMI CCSGG 1 cut(s) 1483
BsaJI CCNNGG 3 cut(s) 178, 732, 1309
Bsc4I CCNNNNNNNGG 2 cut(s) 992, 1310
Bse118I RCCGGY 2 cut(s) 80, 1604
Bse1I ACTGG 6 cut(s) 304, 460, 824, 860, 894, 1641
Bse3DI GCAATG 2 cut(s) 1050, 1293
BseBI CCWGG 2 cut(s) 923, 1311
BseDI CCNNGG 3 cut(s) 178, 732, 1309
BseGI GGATG 3 cut(s) 808, 1491, 1536
BseLI CCNNNNNNNGG 2 cut(s) 992, 1310
BseMI GCAATG 2 cut(s) 1050, 1293
BseMII CTCAG 4 cut(s) 377, 605, 1200, 1380
BseNI ACTGG 6 cut(s) 304, 460, 824, 860, 894, 1641
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 2 cut(s) 535, 697
BsgI GTGCAG 1 cut(s) 41
BshFI GGCC 3 cut(s) 356, 921, 1604
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 2 cut(s) 1360, 1627
BsiHKCI CYCGRG 1 cut(s) 1451
BsiSI CCGG 4 cut(s) 81, 720, 1482, 1605
BslI CCNNNNNNNGG 2 cut(s) 992, 1310
BsmAI GTCTC 2 cut(s) 1122, 1210
BsnI GGCC 3 cut(s) 356, 921, 1604
BsoBI CYCGRG 1 cut(s) 1451
Bsp1286I GDGCHC 4 cut(s) 388, 1360, 1404, 1627
Bsp143I GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
BspACI CCGC 2 cut(s) 951, 1431
BspANI GGCC 3 cut(s) 356, 921, 1604
BspCNI CTCAG 4 cut(s) 376, 606, 1201, 1381
BspHI TCATGA 2 cut(s) 279, 1248
BspLI GGNNCC 3 cut(s) 79, 355, 385
BspMI ACCTGC 2 cut(s) 13, 194
BspOI GCTAGC 1 cut(s) 1442
BspQI GCTCTTC 1 cut(s) 774
BspT107I GGYRCC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 1050, 1293
BsrFI RCCGGY 2 cut(s) 80, 1604
BsrI ACTGG 6 cut(s) 304, 460, 824, 860, 894, 1641
BssAI RCCGGY 2 cut(s) 80, 1604
BssECI CCNNGG 3 cut(s) 178, 732, 1309
BssMI GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
BssNAI GTATAC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 705
BssT1I CCWWGG 1 cut(s) 178
Bst1107I GTATAC 1 cut(s) 571
Bst2BI CACGAG 1 cut(s) 705
Bst2UI CCWGG 2 cut(s) 923, 1311
Bst4CI ACNGT 4 cut(s) 49, 733, 792, 1141
Bst6I CTCTTC 1 cut(s) 774
BstC8I GCNNGC 4 cut(s) 7, 291, 786, 1440
BstDEI CTNAG 6 cut(s) 363, 461, 614, 1134, 1209, 1389
BstDSI CCRYGG 1 cut(s) 732
BstEII GGTNACC 1 cut(s) 1477
BstF5I GGATG 3 cut(s) 808, 1491, 1536
BstKTI GATC 7 cut(s) 30, 69, 142, 747, 973, 1498, 1541
BstMAI GTCTC 2 cut(s) 1122, 1210
BstMBI GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
BstMWI GCNNNNNNNGC 2 cut(s) 716, 1399
BstNI CCWGG 2 cut(s) 923, 1311
BstNSI RCATGY 1 cut(s) 9
BstPI GGTNACC 1 cut(s) 1477
BstSCI CCNGG 3 cut(s) 921, 1309, 1481
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 535, 697
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 1 cut(s) 1538
BstYI RGATCY 1 cut(s) 1538
BstZ17I GTATAC 1 cut(s) 571
BsuRI GGCC 3 cut(s) 356, 921, 1604
BtgI CCRYGG 1 cut(s) 732
BtsCI GGATG 3 cut(s) 808, 1491, 1536
BtsI GCAGTG 2 cut(s) 24, 1367
BtsIMutI CAGTG 7 cut(s) 24, 297, 788, 1367, 1551, 1580, 1634
BveI ACCTGC 2 cut(s) 13, 194
Cac8I GCNNGC 4 cut(s) 7, 291, 786, 1440
CciI TCATGA 2 cut(s) 279, 1248
Cfr10I RCCGGY 2 cut(s) 80, 1604
Cfr13I GGNCC 2 cut(s) 175, 354
Csp6I GTAC 2 cut(s) 1029, 1408
CviAII CATG 9 cut(s) 6, 70, 259, 280, 667, 748, 754, 1249, 1275
CviQI GTAC 2 cut(s) 1029, 1408
DdeI CTNAG 6 cut(s) 363, 461, 614, 1134, 1209, 1389
DpnI GATC 7 cut(s) 29, 68, 141, 746, 972, 1497, 1540
DpnII GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
DriI GACNNNNNGTC 1 cut(s) 121
EaeI YGGCCR 1 cut(s) 1602
Eam1104I CTCTTC 1 cut(s) 774
Eam1105I GACNNNNNGTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 774
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 1404
Eco32I GATATC 1 cut(s) 1127
Eco47I GGWCC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 1527
Eco88I CYCGRG 1 cut(s) 1451
Eco91I GGTNACC 1 cut(s) 1477
EcoO109I RGGNCCY 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 1477
EcoRII CCWGG 2 cut(s) 921, 1309
EcoRV GATATC 1 cut(s) 1127
EcoT14I CCWWGG 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 1404
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 9 cut(s) 9, 73, 262, 283, 670, 751, 757, 1252, 1278
FatI CATG 9 cut(s) 5, 69, 258, 279, 666, 747, 753, 1248, 1274
FbaI TGATCA 4 cut(s) 27, 66, 139, 744
FblI GTMKAC 2 cut(s) 570, 1092
Fnu4HI GCNGC 2 cut(s) 549, 711
FokI GGATG 3 cut(s) 795, 1498, 1543
FriOI GRGCYC 1 cut(s) 1404
Fsp4HI GCNGC 2 cut(s) 549, 711
FspBI CTAG 3 cut(s) 245, 1439, 1656
GluI GCNGC 2 cut(s) 549, 711
GsuI CTGGAG 2 cut(s) 96, 807
HaeIII GGCC 3 cut(s) 356, 921, 1604
HapII CCGG 4 cut(s) 81, 720, 1482, 1605
Hin1II CATG 9 cut(s) 9, 73, 262, 283, 670, 751, 757, 1252, 1278
HindIII AAGCTT 1 cut(s) 1503
HpaII CCGG 4 cut(s) 81, 720, 1482, 1605
Hpy166II GTNNAC 5 cut(s) 45, 126, 376, 571, 1093
Hpy188I TCNGA 6 cut(s) 66, 366, 776, 1107, 1210, 1390
Hpy188III TCNNGA 8 cut(s) 280, 403, 802, 866, 875, 884, 1249, 1499
Hpy8I GTNNAC 5 cut(s) 45, 126, 376, 571, 1093
HpyAV CCTTC 2 cut(s) 1504, 1543
HpyCH4III ACNGT 4 cut(s) 49, 733, 792, 1141
HpyF10VI GCNNNNNNNGC 2 cut(s) 716, 1399
HpyF3I CTNAG 6 cut(s) 363, 461, 614, 1134, 1209, 1389
Hsp92II CATG 9 cut(s) 9, 73, 262, 283, 670, 751, 757, 1252, 1278
Ksp22I TGATCA 4 cut(s) 27, 66, 139, 744
Kzo9I GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 3 cut(s) 77, 467, 1552
Lsp1109I GCAGC 2 cut(s) 535, 697
LweI GCATC 1 cut(s) 1476
MaeI CTAG 3 cut(s) 245, 1439, 1656
MaeIII GTNAC 5 cut(s) 220, 295, 391, 1477, 1609
MalI GATC 7 cut(s) 29, 68, 141, 746, 972, 1497, 1540
MboI GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
MboII GAAGA 6 cut(s) 48, 160, 233, 791, 1010, 1533
MfeI CAATTG 1 cut(s) 1361
MflI RGATCY 1 cut(s) 1538
MhlI GDGCHC 4 cut(s) 388, 1360, 1404, 1627
MlyI GAGTC 5 cut(s) 204, 627, 670, 1124, 1453
MmeI TCCRAC 1 cut(s) 251
MseI TTAA 2 cut(s) 443, 1425
MslI CAYNNNNRTG 5 cut(s) 671, 731, 752, 1151, 1484
MspI CCGG 4 cut(s) 81, 720, 1482, 1605
MspR9I CCNGG 3 cut(s) 923, 1311, 1483
MunI CAATTG 1 cut(s) 1361
MvaI CCWGG 2 cut(s) 923, 1311
MwoI GCNNNNNNNGC 2 cut(s) 716, 1399
NciI CCSGG 1 cut(s) 1483
NdeII GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
NheI GCTAGC 1 cut(s) 1438
NlaIII CATG 9 cut(s) 9, 73, 262, 283, 670, 751, 757, 1252, 1278
NlaIV GGNNCC 3 cut(s) 79, 355, 385
NmuCI GTSAC 4 cut(s) 295, 391, 1477, 1609
NspI RCATGY 1 cut(s) 9
OliI CACNNNNGTG 2 cut(s) 731, 1151
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 2 cut(s) 279, 1248
PaqCI CACCTGC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 774
PfeI GAWTC 5 cut(s) 509, 523, 796, 818, 893
PflMI CCANNNNNTGG 1 cut(s) 1310
PkrI GCNGC 2 cut(s) 550, 712
PleI GAGTC 5 cut(s) 203, 626, 670, 1123, 1452
PpsI GAGTC 5 cut(s) 203, 626, 670, 1123, 1452
PpuMI RGGWCCY 1 cut(s) 175
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 2 cut(s) 921, 1309
PspEI GGTNACC 1 cut(s) 1477
PspGI CCWGG 2 cut(s) 921, 1309
PspN4I GGNNCC 3 cut(s) 79, 355, 385
PspPI GGNCC 2 cut(s) 175, 354
PspPPI RGGWCCY 1 cut(s) 175
PsuI RGATCY 1 cut(s) 1538
RsaI GTAC 2 cut(s) 1030, 1409
RsaNI GTAC 2 cut(s) 1029, 1408
RseI CAYNNNNRTG 5 cut(s) 671, 731, 752, 1151, 1484
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 2 cut(s) 443, 1425
SatI GCNGC 2 cut(s) 549, 711
Sau3AI GATC 7 cut(s) 27, 66, 139, 744, 970, 1495, 1538
Sau96I GGNCC 2 cut(s) 175, 354
ScaI AGTACT 1 cut(s) 1409
SchI GAGTC 5 cut(s) 204, 627, 670, 1124, 1453
ScrFI CCNGG 3 cut(s) 923, 1311, 1483
SduI GDGCHC 4 cut(s) 388, 1360, 1404, 1627
SfaNI GCATC 1 cut(s) 1476
SinI GGWCC 1 cut(s) 175
SmiMI CAYNNNNRTG 5 cut(s) 671, 731, 752, 1151, 1484
SmlI CTYRAG 2 cut(s) 800, 1403
SmoI CTYRAG 2 cut(s) 800, 1403
SphI GCATGC 1 cut(s) 9
SsiI CCGC 2 cut(s) 951, 1431
SspI AATATT 1 cut(s) 474
SspMI CTAG 3 cut(s) 245, 1439, 1656
StyD4I CCNGG 3 cut(s) 921, 1309, 1481
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 4 cut(s) 49, 733, 792, 1141
TaqI TCGA 1 cut(s) 1498
TatI WGTACW 1 cut(s) 1407
TfiI GAWTC 5 cut(s) 509, 523, 796, 818, 893
Tru1I TTAA 2 cut(s) 443, 1425
Tru9I TTAA 2 cut(s) 443, 1425
TscAI CASTG 7 cut(s) 24, 304, 795, 1374, 1551, 1580, 1641
TseFI GTSAC 4 cut(s) 295, 391, 1477, 1609
TseI GCWGC 2 cut(s) 548, 710
Tsp45I GTSAC 4 cut(s) 295, 391, 1477, 1609
TspDTI ATGAA 5 cut(s) 268, 275, 602, 742, 1039
TspGWI ACGGA 1 cut(s) 1046
TspRI CASTG 7 cut(s) 24, 304, 795, 1374, 1551, 1580, 1641
Van91I CCANNNNNTGG 1 cut(s) 1310
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 6 cut(s) 262, 367, 406, 483, 870, 879
XceI RCATGY 1 cut(s) 9
XmiI GTMKAC 2 cut(s) 570, 1092
XspI CTAG 3 cut(s) 245, 1439, 1656
ZrmI AGTACT 1 cut(s) 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.