Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0002027

Physical Location & Seq

Genomic Coordinates Reverse (-)

61478819 .. 61481093

Gene Structure 2275 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG385400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGCATGCCAATTTGCAGTGCAGGTGATCAAAGGAAGATGGTTCACCGTGTTTGCCTCGTTTCTGATCATGGCTGGAGCCGGTGCTACTTATCTGTTTGGTTCTTATTCCAAAGACATCAAGTCCACTCTTGGCTATGATCAATCAACTCTCAACTTGATTGGCTTCTTCAAGGACCTTGGTGCAAATGTGGGAGTCCTCGCAGGCCTAATAGGTGAAGTAACTCCAACTTGGTTTGTTCTTCTAGTTGGTTCTGCCATGAATTTTGCAGGGTATTTCATGATTTGGCTTGCTGTCACTGGCAAAATAGCCAAACCTAAAGTCTGGCAGATGTGCCTTTACATCTGCGTTGGGGCCAATTCTCAGAATTTTGTAAACACAGGTGCCCTTGTCACTTGTGTCAAGAATTTCCCAGAAAGTAGAGGTGTTATGCTTGGTTTGTTAAAGGGTTATACTGGGCTTAGTGGAGCAATATTCACCCAAATTTACTTGGCTATTTACCCAAACGATTCAAAGTCTTTGATTCTTTTGATTGGTTGGCTTCCTGCTGCTCTTTCAGTCATTTTTGTATACACCATTCGTCCAATGAAAGTTGTTAGGCAACAAAATGAGCTGAGAGTCTTTTACCATTTCCTCTATATCTCACTTTCTCTTGGAGTTTTCCTCATGGTAATGACTCTGCTTGAAAAAACAATCACTTTCTCTCGTGCTGCTTATGCCGGAAGCACCACCGTGGTTTGTATGATCATGTTCATGCCTCTTGGAATTGCAATCAGAGAAGAGCTTGCACTGTGGAATCTCAAGAAACATCCTGTTGATTCTCCAGTTGAGGTGATCAGCATTGATAAAAGACCAAACCCAGTTCCAGAAATTCAAGAACTGGATTCTTTCCCACCCAAATCCTCATTAGGCCAGGAAAAGAAAACACAGATTACTACTACCACTACATCTTGCTGTGGTGATATATGCAATAAGCCAGAAAGAGGGGAAGACTACACAATACTACAAGCACTTTTGAGTACGGATATGATTATTCTATTCATTGCAACTTTTTGTGGGTTGGGGTCAAGTTTGACAGCAGTAGACAATTTGGGTCAGATTGGTGAGTCTCTGGGATATCCCACTAAGACAGTAGGCACTTTTGTGTCATTAGTGAGCATTTGGAATTATTTTGGGAGGGTCTTTTCTGGTTTTGTCTCTGAAAGCCTAATGGTGAAGTGGAAAATCCCCAGAACACTCATGATGACCTTTATGCTTGTTCTATCATGTATTGCATATCTCTCCATTGCTTTCCCATTCCCTGGTTCAGTTTATATGGCTTCGGTTATGATTGGGGTTTCTTTTGGTGCTCAATTGACACTGCTTTTCACAATCATATCTGAGCTGTTTGGGCTCAAGTACTATGCTACATTGTTCAACTGCGGACAGCTAGCGAGTCCTCTCGGGTCTTACATTCTCAATGTGAGGGTCACCGGGATGCTCTATGATCGAGAAGCTTTGAAGGTGTTGGCAAAGAAAGGGATGACAAGATCTTCAGTGAAGGAGCTGGTTTGCATTGGAAGTCAGTGTTATAGGCTGTCTTTTACAATTTTGGCCGGTATCACATTCTTTGGTGCTCTTATTTCACTGGTTTTGGTGATTAGGACTAGAGAGTTCTACAAAGGTGATATATACAAGAAGTATAGAGAAGTTGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 Nodulin-like

Protein Analysis

565

Amino Acids

62.37

Weight (kDa)

9.18

Isoelectric Point (pI)

29.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nodulin-like	PF06813	13 - 257	1e-94	Nodulin-like
MFS_1	PF07690	335 - 472	2.6e-06	Major Facilitator Superfamily
NFD4_C	PF23262	342 - 553	5.3e-15	NFD4, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28120
fragaria_vesca	FvH4_3g30820 FvH4_3g30830
malus_domestica	MD03G1126600.v1.1 MD04G1112900.v1.1 MD11G1146900.v1.1 MD11G1147000.v1.1 MD11G1147200.v1.1 MD12G1129200.v1.1
prunus_persica	Prupe.6G110900_v2.0.a1 Prupe.6G111100_v2.0.a1 Prupe.6G111300_v2.0.a1
pyrus_communis	pycom04g10260 pycom04g10300 pycom11g12040
rosa_chinensis	RchiOBHm_Chr5g0056971 RchiOBHm_Chr5g0057001 RchiOBHm_Chr5g0057021
rosa_laevigata	RLG00000035144 RLG00000035145 RLG00000035147
rosa_multiflora	Rmu_sc0002035.1_g000001 Rmu_sc0009185.1_g000006 Rmu_sc0009185.1_g000013
rosa_roxburghii	Rroxscaffold_1G00023150 Rroxscaffold_1G00023190 Rroxscaffold_1G00023200
rosa_rugosa	Rorug05G0305000 Rorug05G0305100 Rorug05G0305200
rosa_samantha	Rh5AG374200 Rh5AG374500 Rh5AG374600 Rh5BG385300 Rh5BG385400 Rh5BG385700 Rh5CG408500 Rh5CG408900 Rh5CG409100 Rh5DG398600 Rh5DG398900 Rh5DG399100
rosa_wichuraiana	Rw5G035230 Rw5G035250 Rw5G035260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	13
Acc36I	ACCTGC	1 cut(s)	13
AccB1I	GGYRCC	1 cut(s)	383
AccB7I	CCANNNNNTGG	1 cut(s)	1301
AccI	GTMKAC	2 cut(s)	570, 1083
AciI	CCGC	1 cut(s)	1422
AcoI	YGGCCR	1 cut(s)	1593
AcsI	RAATTY	5 cut(s)	262, 367, 406, 483, 870
AcuI	CTGAAG	1 cut(s)	1518
AfaI	GTAC	2 cut(s)	1021, 1400
AfiI	CCNNNNNNNGG	2 cut(s)	983, 1301
AgsI	TTSAA	6 cut(s)	172, 513, 686, 875, 1417, 1501
AhdI	GACNNNNNGTC	1 cut(s)	121
AjnI	CCWGG	2 cut(s)	912, 1300
AleI	CACNNNNGTG	2 cut(s)	731, 1142
AluBI	AGCT	6 cut(s)	613, 784, 1384, 1429, 1496, 1546
AluI	AGCT	6 cut(s)	613, 784, 1384, 1429, 1496, 1546
Alw21I	GWGCWC	2 cut(s)	1351, 1618
Alw26I	GTCTC	2 cut(s)	1113, 1201
Ama87I	CYCGRG	1 cut(s)	1442
AoxI	GGCC	4 cut(s)	205, 354, 910, 1593
ApeKI	GCWGC	2 cut(s)	548, 710
ApoI	RAATTY	5 cut(s)	262, 367, 406, 483, 870
ArsI	GACNNNNNNTTYG	2 cut(s)	306, 338
AspS9I	GGNCC	2 cut(s)	175, 354
AsuC2I	CCSGG	1 cut(s)	1474
AsuNHI	GCTAGC	1 cut(s)	1429
AvaI	CYCGRG	1 cut(s)	1442
AvaII	GGWCC	1 cut(s)	175
BaeGI	GKGCMC	1 cut(s)	388
BanI	GGYRCC	1 cut(s)	383
BanII	GRGCYC	1 cut(s)	1395
BauI	CACGAG	1 cut(s)	705
BbsI	GAAGAC	1 cut(s)	996
Bbv12I	GWGCWC	2 cut(s)	1351, 1618
BbvI	GCAGC	2 cut(s)	535, 697
BccI	CCATC	1 cut(s)	33
BciT130I	CCWGG	2 cut(s)	914, 1302
BclI	TGATCA	5 cut(s)	27, 66, 139, 744, 834
BcnI	CCSGG	1 cut(s)	1474
BcoDI	GTCTC	2 cut(s)	1113, 1201
BfaI	CTAG	3 cut(s)	245, 1430, 1647
BfuAI	ACCTGC	1 cut(s)	13
BglII	AGATCT	1 cut(s)	1529
BisI	GCNGC	2 cut(s)	549, 711
BlsI	GCNGC	2 cut(s)	550, 712
BmcAI	AGTACT	1 cut(s)	1400
Bme1390I	CCNGG	3 cut(s)	914, 1302, 1474
Bme18I	GGWCC	1 cut(s)	175
BmeRI	GACNNNNNGTC	1 cut(s)	121
BmeT110I	CYCGRG	1 cut(s)	1442
BmgT120I	GGNCC	2 cut(s)	175, 354
BmiI	GGNNCC	3 cut(s)	79, 355, 385
BmrFI	CCNGG	3 cut(s)	914, 1302, 1474
BmrI	ACTGGG	2 cut(s)	465, 854
BmsI	GCATC	1 cut(s)	1467
BmtI	GCTAGC	1 cut(s)	1433
BmuI	ACTGGG	2 cut(s)	465, 854
BpiI	GAAGAC	1 cut(s)	996
BplI	GAGNNNNNCTC	2 cut(s)	648, 680
BpmI	CTGGAG	2 cut(s)	96, 807
BpuEI	CTTGAG	2 cut(s)	785, 1379
BpuMI	CCSGG	1 cut(s)	1474
BsaJI	CCNNGG	3 cut(s)	178, 732, 1300
Bsc4I	CCNNNNNNNGG	2 cut(s)	983, 1301
Bse118I	RCCGGY	2 cut(s)	80, 1595
Bse1I	ACTGG	6 cut(s)	304, 460, 824, 860, 885, 1632
Bse3DI	GCAATG	2 cut(s)	1041, 1284
BseBI	CCWGG	2 cut(s)	914, 1302
BseDI	CCNNGG	3 cut(s)	178, 732, 1300
BseGI	GGATG	3 cut(s)	808, 1482, 1527
BseLI	CCNNNNNNNGG	2 cut(s)	983, 1301
BseMI	GCAATG	2 cut(s)	1041, 1284
BseMII	CTCAG	3 cut(s)	377, 605, 1371
BseNI	ACTGG	6 cut(s)	304, 460, 824, 860, 885, 1632
BseSI	GKGCMC	1 cut(s)	388
BseXI	GCAGC	2 cut(s)	535, 697
BsgI	GTGCAG	1 cut(s)	41
BshFI	GGCC	4 cut(s)	207, 356, 912, 1595
BshNI	GGYRCC	1 cut(s)	383
BsiHKAI	GWGCWC	2 cut(s)	1351, 1618
BsiHKCI	CYCGRG	1 cut(s)	1442
BsiSI	CCGG	4 cut(s)	81, 720, 1473, 1596
BslI	CCNNNNNNNGG	2 cut(s)	983, 1301
BsmAI	GTCTC	2 cut(s)	1113, 1201
BsnI	GGCC	4 cut(s)	207, 356, 912, 1595
BsoBI	CYCGRG	1 cut(s)	1442
Bsp1286I	GDGCHC	4 cut(s)	388, 1351, 1395, 1618
Bsp143I	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
BspACI	CCGC	1 cut(s)	1422
BspANI	GGCC	4 cut(s)	207, 356, 912, 1595
BspCNI	CTCAG	3 cut(s)	376, 606, 1372
BspHI	TCATGA	2 cut(s)	279, 1239
BspLI	GGNNCC	3 cut(s)	79, 355, 385
BspMI	ACCTGC	1 cut(s)	13
BspOI	GCTAGC	1 cut(s)	1433
BspQI	GCTCTTC	1 cut(s)	774
BspT107I	GGYRCC	1 cut(s)	383
BsrDI	GCAATG	2 cut(s)	1041, 1284
BsrFI	RCCGGY	2 cut(s)	80, 1595
BsrI	ACTGG	6 cut(s)	304, 460, 824, 860, 885, 1632
BssAI	RCCGGY	2 cut(s)	80, 1595
BssECI	CCNNGG	3 cut(s)	178, 732, 1300
BssMI	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
BssNAI	GTATAC	1 cut(s)	571
BssSI	CACGAG	1 cut(s)	705
BssT1I	CCWWGG	1 cut(s)	178
Bst1107I	GTATAC	1 cut(s)	571
Bst2BI	CACGAG	1 cut(s)	705
Bst2UI	CCWGG	2 cut(s)	914, 1302
Bst4CI	ACNGT	4 cut(s)	49, 733, 792, 1132
Bst6I	CTCTTC	1 cut(s)	774
BstC8I	GCNNGC	5 cut(s)	7, 205, 291, 786, 1431
BstDEI	CTNAG	5 cut(s)	363, 461, 614, 1125, 1380
BstDSI	CCRYGG	1 cut(s)	732
BstEII	GGTNACC	1 cut(s)	1468
BstF5I	GGATG	3 cut(s)	808, 1482, 1527
BstKTI	GATC	7 cut(s)	30, 69, 142, 747, 837, 1489, 1532
BstMAI	GTCTC	2 cut(s)	1113, 1201
BstMBI	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
BstMWI	GCNNNNNNNGC	2 cut(s)	716, 1390
BstNI	CCWGG	2 cut(s)	914, 1302
BstNSI	RCATGY	1 cut(s)	9
BstPI	GGTNACC	1 cut(s)	1468
BstSCI	CCNGG	3 cut(s)	912, 1300, 1472
BstSLI	GKGCMC	1 cut(s)	388
BstV1I	GCAGC	2 cut(s)	535, 697
BstV2I	GAAGAC	1 cut(s)	996
BstX2I	RGATCY	1 cut(s)	1529
BstYI	RGATCY	1 cut(s)	1529
BstZ17I	GTATAC	1 cut(s)	571
BsuRI	GGCC	4 cut(s)	207, 356, 912, 1595
BtgI	CCRYGG	1 cut(s)	732
BtsCI	GGATG	3 cut(s)	808, 1482, 1527
BtsI	GCAGTG	2 cut(s)	24, 1358
BtsIMutI	CAGTG	7 cut(s)	24, 297, 788, 1358, 1542, 1571, 1625
BveI	ACCTGC	1 cut(s)	13
Cac8I	GCNNGC	5 cut(s)	7, 205, 291, 786, 1431
CciI	TCATGA	2 cut(s)	279, 1239
Cfr10I	RCCGGY	2 cut(s)	80, 1595
Cfr13I	GGNCC	2 cut(s)	175, 354
Csp6I	GTAC	2 cut(s)	1020, 1399
CviAII	CATG	9 cut(s)	6, 70, 259, 280, 667, 748, 754, 1240, 1266
CviQI	GTAC	2 cut(s)	1020, 1399
DdeI	CTNAG	5 cut(s)	363, 461, 614, 1125, 1380
DpnI	GATC	7 cut(s)	29, 68, 141, 746, 836, 1488, 1531
DpnII	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
DriI	GACNNNNNGTC	1 cut(s)	121
EaeI	YGGCCR	1 cut(s)	1593
Eam1104I	CTCTTC	1 cut(s)	774
Eam1105I	GACNNNNNGTC	1 cut(s)	121
EarI	CTCTTC	1 cut(s)	774
Eco130I	CCWWGG	1 cut(s)	178
Eco147I	AGGCCT	1 cut(s)	207
Eco24I	GRGCYC	1 cut(s)	1395
Eco32I	GATATC	1 cut(s)	1118
Eco47I	GGWCC	1 cut(s)	175
Eco57I	CTGAAG	1 cut(s)	1518
Eco88I	CYCGRG	1 cut(s)	1442
Eco91I	GGTNACC	1 cut(s)	1468
EcoO109I	RGGNCCY	1 cut(s)	175
EcoO65I	GGTNACC	1 cut(s)	1468
EcoRII	CCWGG	2 cut(s)	912, 1300
EcoRV	GATATC	1 cut(s)	1118
EcoT14I	CCWWGG	1 cut(s)	178
EcoT38I	GRGCYC	1 cut(s)	1395
ErhI	CCWWGG	1 cut(s)	178
FaeI	CATG	9 cut(s)	9, 73, 262, 283, 670, 751, 757, 1243, 1269
FatI	CATG	9 cut(s)	5, 69, 258, 279, 666, 747, 753, 1239, 1265
FbaI	TGATCA	5 cut(s)	27, 66, 139, 744, 834
FblI	GTMKAC	2 cut(s)	570, 1083
Fnu4HI	GCNGC	2 cut(s)	549, 711
FokI	GGATG	3 cut(s)	795, 1489, 1534
FriOI	GRGCYC	1 cut(s)	1395
Fsp4HI	GCNGC	2 cut(s)	549, 711
FspBI	CTAG	3 cut(s)	245, 1430, 1647
GluI	GCNGC	2 cut(s)	549, 711
GsuI	CTGGAG	2 cut(s)	96, 807
HaeIII	GGCC	4 cut(s)	207, 356, 912, 1595
HapII	CCGG	4 cut(s)	81, 720, 1473, 1596
Hin1II	CATG	9 cut(s)	9, 73, 262, 283, 670, 751, 757, 1243, 1269
HindIII	AAGCTT	1 cut(s)	1494
HpaII	CCGG	4 cut(s)	81, 720, 1473, 1596
Hpy166II	GTNNAC	5 cut(s)	45, 126, 376, 571, 1084
Hpy188I	TCNGA	6 cut(s)	66, 366, 776, 1098, 1201, 1381
Hpy188III	TCNNGA	7 cut(s)	280, 403, 802, 866, 875, 1240, 1490
Hpy8I	GTNNAC	5 cut(s)	45, 126, 376, 571, 1084
HpyAV	CCTTC	2 cut(s)	1495, 1534
HpyCH4III	ACNGT	4 cut(s)	49, 733, 792, 1132
HpyF10VI	GCNNNNNNNGC	2 cut(s)	716, 1390
HpyF3I	CTNAG	5 cut(s)	363, 461, 614, 1125, 1380
Hsp92II	CATG	9 cut(s)	9, 73, 262, 283, 670, 751, 757, 1243, 1269
Ksp22I	TGATCA	5 cut(s)	27, 66, 139, 744, 834
Kzo9I	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
LguI	GCTCTTC	1 cut(s)	774
LmnI	GCTCC	3 cut(s)	77, 467, 1543
Lsp1109I	GCAGC	2 cut(s)	535, 697
LweI	GCATC	1 cut(s)	1467
MaeI	CTAG	3 cut(s)	245, 1430, 1647
MaeIII	GTNAC	4 cut(s)	220, 295, 391, 1468
MalI	GATC	7 cut(s)	29, 68, 141, 746, 836, 1488, 1531
MboI	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
MboII	GAAGA	6 cut(s)	48, 160, 233, 791, 1001, 1524
MfeI	CAATTG	1 cut(s)	1352
MflI	RGATCY	1 cut(s)	1529
MhlI	GDGCHC	4 cut(s)	388, 1351, 1395, 1618
MlyI	GAGTC	5 cut(s)	204, 627, 670, 1115, 1444
MmeI	TCCRAC	1 cut(s)	251
MseI	TTAA	1 cut(s)	443
MslI	CAYNNNNRTG	5 cut(s)	671, 731, 752, 1142, 1475
MspI	CCGG	4 cut(s)	81, 720, 1473, 1596
MspR9I	CCNGG	3 cut(s)	914, 1302, 1474
MunI	CAATTG	1 cut(s)	1352
MvaI	CCWGG	2 cut(s)	914, 1302
MwoI	GCNNNNNNNGC	2 cut(s)	716, 1390
NciI	CCSGG	1 cut(s)	1474
NdeII	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
NheI	GCTAGC	1 cut(s)	1429
NlaIII	CATG	9 cut(s)	9, 73, 262, 283, 670, 751, 757, 1243, 1269
NlaIV	GGNNCC	3 cut(s)	79, 355, 385
NmuCI	GTSAC	3 cut(s)	295, 391, 1468
NspI	RCATGY	1 cut(s)	9
OliI	CACNNNNGTG	2 cut(s)	731, 1142
PaeI	GCATGC	1 cut(s)	9
PagI	TCATGA	2 cut(s)	279, 1239
PaqCI	CACCTGC	1 cut(s)	13
PceI	AGGCCT	1 cut(s)	207
PciSI	GCTCTTC	1 cut(s)	774
PfeI	GAWTC	5 cut(s)	509, 523, 796, 818, 884
PflMI	CCANNNNNTGG	1 cut(s)	1301
PkrI	GCNGC	2 cut(s)	550, 712
PleI	GAGTC	5 cut(s)	203, 626, 670, 1114, 1443
PpsI	GAGTC	5 cut(s)	203, 626, 670, 1114, 1443
PpuMI	RGGWCCY	1 cut(s)	175
Psp5II	RGGWCCY	1 cut(s)	175
Psp6I	CCWGG	2 cut(s)	912, 1300
PspEI	GGTNACC	1 cut(s)	1468
PspGI	CCWGG	2 cut(s)	912, 1300
PspN4I	GGNNCC	3 cut(s)	79, 355, 385
PspPI	GGNCC	2 cut(s)	175, 354
PspPPI	RGGWCCY	1 cut(s)	175
PsuI	RGATCY	1 cut(s)	1529
RsaI	GTAC	2 cut(s)	1021, 1400
RsaNI	GTAC	2 cut(s)	1020, 1399
RseI	CAYNNNNRTG	5 cut(s)	671, 731, 752, 1142, 1475
SapI	GCTCTTC	1 cut(s)	774
SaqAI	TTAA	1 cut(s)	443
SatI	GCNGC	2 cut(s)	549, 711
Sau3AI	GATC	7 cut(s)	27, 66, 139, 744, 834, 1486, 1529
Sau96I	GGNCC	2 cut(s)	175, 354
ScaI	AGTACT	1 cut(s)	1400
SchI	GAGTC	5 cut(s)	204, 627, 670, 1115, 1444
ScrFI	CCNGG	3 cut(s)	914, 1302, 1474
SduI	GDGCHC	4 cut(s)	388, 1351, 1395, 1618
SfaNI	GCATC	1 cut(s)	1467
SinI	GGWCC	1 cut(s)	175
SmiMI	CAYNNNNRTG	5 cut(s)	671, 731, 752, 1142, 1475
SmlI	CTYRAG	2 cut(s)	800, 1394
SmoI	CTYRAG	2 cut(s)	800, 1394
SphI	GCATGC	1 cut(s)	9
SseBI	AGGCCT	1 cut(s)	207
SsiI	CCGC	1 cut(s)	1422
SspI	AATATT	1 cut(s)	474
SspMI	CTAG	3 cut(s)	245, 1430, 1647
StuI	AGGCCT	1 cut(s)	207
StyD4I	CCNGG	3 cut(s)	912, 1300, 1472
StyI	CCWWGG	1 cut(s)	178
TaaI	ACNGT	4 cut(s)	49, 733, 792, 1132
TaqI	TCGA	1 cut(s)	1489
TatI	WGTACW	1 cut(s)	1398
TfiI	GAWTC	5 cut(s)	509, 523, 796, 818, 884
Tru1I	TTAA	1 cut(s)	443
Tru9I	TTAA	1 cut(s)	443
TscAI	CASTG	7 cut(s)	24, 304, 795, 1365, 1542, 1571, 1632
TseFI	GTSAC	3 cut(s)	295, 391, 1468
TseI	GCWGC	2 cut(s)	548, 710
Tsp45I	GTSAC	3 cut(s)	295, 391, 1468
TspDTI	ATGAA	5 cut(s)	268, 275, 602, 742, 1030
TspGWI	ACGGA	1 cut(s)	1037
TspRI	CASTG	7 cut(s)	24, 304, 795, 1365, 1542, 1571, 1632
Van91I	CCANNNNNTGG	1 cut(s)	1301
VpaK11BI	GGWCC	1 cut(s)	175
XapI	RAATTY	5 cut(s)	262, 367, 406, 483, 870
XceI	RCATGY	1 cut(s)	9
XmiI	GTMKAC	2 cut(s)	570, 1083
XspI	CTAG	3 cut(s)	245, 1430, 1647
ZrmI	AGTACT	1 cut(s)	1400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.