pycom01g05170

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007148
Physical Location & Seq
Forward (+)
4765590 .. 4771279
5690 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4473 bp
ATGATCGATCTACCTGAATCTAAAGTGTATGTTTTGGATGCTTTAAAAAGTATGCTGTCCGTGGTCCCCCTCAATGATATATCACGTGAAGGTAGTGCTGCTAATGATGCAATTGAGACAATGATAAAAATATTGAGCACAACTAAAGAAGAGACTCAAGCCAAGTCTGCATCAGCTTTAGCTGGAATATTTGAATCCAGGAAGGACTTGCGTGAAAGTAGCATTGCTGTTAAAACTCTTTGGTCAGCCATCAAGTTGATTAGTGTCGAATCTGTATATATCCTTGCAGAGGCCTCACGTTGCCTTGCGGCAATATTTCTTTCAATTAAAGAGAACCGGGATGTGGCAGCTGTTGCTCGAGATATATTATCCCCATTAGTTCTGCTTGCTAACTCTTCAGTTCTGGAAGTTGCAGAACTAGCAACATGTGCTCTGGCTAATCTTATTTTGGACAGTGAAGTTTCAGAGAAAGCAGTTGCTGAAGATGTTATTTTCCCGGCTACTAGAGTTTTGCGTGAAGGCACAGTACCTGGAAAGACCCATGCAGCAGCAGCAATTGCTCGCCTGCTCCATTCTCGTCAAATTGATTATGCTTTAACTGATTGTGTGAATCGTGCTGGAACTGTTCTTGCATTAGTTTCTTTTCTAGAATCTGTTAATCATGATTCTGTTGCCACATCAGAGGCACTTGAGGCACTTGCTATTCTATCCAGGTCAGAAGGTGCTTCTGGAGATATAAAACCTGCATGGGCAGTTTTGGCTGAATTCCCGAAAAGTATTACCCCGATAGTTTTATCCATTGCTGATGCAACACCCTTGTTGCAGGATAAGGCTATTGAAATTTTATCACGGCTTTGCAGAGATCAGCCTGATGTATTGGGTGACACAGTTGCTACTGCTTATGGATGTATTTCATCAATTGCCAAAAGGGTGATCAATTCAACAAAATCAAAAGTTAAAACTGGAGGAACTGCACTACTTATTTGTGTTGCAAAAGTTAGTCATCAGAGAGTTGTGGAAGATCTTAGTGAATCAAACTTACGCACCCAGCTAATTCAAGCTCTTGTTGCAATGCTTAGTTCTTTGGGAAGCCCAGGGGATAATGAGAATGATTCTATTGGCATTTATAGGCATGCTAAAGAGGAAACAAAGATTGATGAGTCTTATTCAAGCACAGGAGTAATTAGTGGTGTCAATTTAGCTATGTGGCTACTGTCTATTCTTGCCTGTCACGATGAAAGGTGCAAATTTGTGATTATGGAGGCTGGGGCTGTGGAAGTCCTCACTGACAGGATCTCAAATGATTTCTCACCCTACTCACAGATTGAGTTTAAAGAAGATAGTAGCATATGGATTTGTACTTTGCTGTTGGCAATCTTGTTTCAAAACAGAGACATCATACGAGCACATGCAACCATGAAATCTGTACCAGTACTTGCAAACTGGTTGAGATCAGAAGATTTGCCCACCAGATATTTTGCTGCACAGGCGATGGCCAGCCTATTGTCAGAAGAGTGTGGTTTAGTACGTTATCCTGAGCAAGTTGCCCTTGAGAGGTTGTTCAGAGTTGAAGACATAAGGGTTGGTGCTACTTCCAGGAAAGCAATACCTGCACTTGTTGATCTACTCAAACCCATTCCAGATCGTCCTGGAGCACCATTTTTAGCACTTGGGCTTCTGACTCAGCTTGCGAAAGACTGTCCATCGAATAAAATTGTAATGGTAGAATCAGGAGCTTTGGAAGCACTGACCAAATATCTTTCACTTGGCCCTCAAGATGCAACTGAGGAAGCTGCTACAGATCTGTTAGGTATACTATTTGGCAGTGCTGAAATCAGGAGACATGACTCTTCATTTGGTGCTGTTGGTCAGCTTGTGGCAGTTTTACGGTTAGGTGGAAGAGCTTCAAGGTACAGTGCTGCTAAAGCATTGGAAAGCTTGTTTTCTGCTGACCATATAAGGAATGCAGAAAGTGCTCGACAAGCTGTTCAACCCTTGGTGGAGATTCTCAATACTGGTTCCGAGAAGGAGCAACATGCTGCAATTGCTGCGTTGGTAAGGCTGCTGAGTGAAAATCCATCAAGAGCCTTGGCAGTTGCAGATGTTGAGATGAATGCAGTAGATGTTCTCTGCAAGATTCTTTCTTCAACTTGCTCAATGGAGCTGAAAGGGGATGCTGCCGAGTTGTGTTGTGTTCTCTTTGGAAATACAAGAATCAGGTCAACAATGGCTGCAGCTAGATGTGTGGAGCCTCTGGTGTCTCTTCTTGTGACTGAGTTCAGTCCTGCACAGCATTCAGTTGTCCGTGCGTTGGATAAACTTGTTGATGATGAGCAACTGGCAGAACTTGTTGCTGCTCACGGGGCAGTTATACCTCTTGTAGGTCTTCTATATGGTAAGAATTACTTGCTCCATGAAGCTATTTCTAGAGCCCTCGTGAAATTAGGGAAAGACAGGCCTGCTTGCAAGATGGAAATGGTGAAAGCTGGAGTAATTGAGAGCATACTTGACATCCTCCATGAGGCACCCGATTTTCTCTCTGCTGCTTTTGCAGAACTGCTCCGAATATTGACCAACAATGCAAGCATTGCTAAGGGACCATCTGCTTCAAAAGTGGTTGAGCCCCTTTTTGTGCTTCTAACAAAACCAGAGTTTGGACCTGATGGGCAGCATAGTGCACTTCAAGTTCTTGTCAACGTTTTGGAGCATCCACAGTGTCGTTCTGACTATAAGTTGACATCCCACCAAGCTGTTGAACCTATTATTCCCTTACTTGATTCTCCTGCTCCAGCAGTACAACAGTTGGCTGCTGAGCTTCTGTCACATTTACTCTTTGAAGAACAACTTCAAAAGGATTCAGTGACTCAGCAAGTGATTGGTCCTCTCATACGGGTTCTTGGCTCTGGTATACACATATTGCAGCAGAGAGCTGTGAAGGCCCGTGTTAGTATTGCGCTAATTTGGCCGAATGAAATAGCAAAAGAGGGTGGTGTCACTGAATTGTCCAAGGTGATATTGCAATCAGATCCATCTCTTCCTCATGACTTGTGGGAATCAGCTGCTGCTGTTTTATCAAGCATTCTGCAGTTTAGTTCTGAATTTTATTTGGAAGTGCCTGTGGCTGTGTTGGTAAGGTTGCTTCGTTCTGGCTCAGAAGGCACAGTAATTGGTGCATTGAATGCTCTTCTGGTACTGGAAAGTGATGATGCAACCAGCGCAGAAGCAATGGCTGAAAGTGGTGCTTTAGAGGCTCTTTTGGATCTTCTTAGATCTCATCAGTGTGAGGAAACTGCTGCTAGGCTGTTGGAAGTATTGTTGAACAATGTGAAGATCAGAGAAACTAAGGCTACCAAGTCTGCTGTATTACCATTGTCTCAGTACCTCTTAGATCCACAAACCCAAGCTCAGCAAGCGAGGTTACTGGCAACTCTAGCTCTTGGTGATCTATTCCAAAATGAGGGCCTTGCTCGAAGCACTGACGCTGTTTCAGCTTGTCGTGCACTAGTAAATGTGCTTGAAGAGCAACCTACTGAAGAAATGAAGGTTGTAGCGATATGTGCTTTGCAGAACCTTGTCATGTACAGTAGATCTAATAAAAGAGCAGTTGCTGAGGCTGGTGGCGTCCAGGTTGTGTTGGATCTGATTGGTGCAAGTTCGATCCAGATACTTCTATTCAGGCTGCAATGTTTGTTAAACTTCTCTTTTCAAACCATACCATTCAAGAGTATGCTTCTAGTGAGACAGTAAAGGGTGATTACTGCTGCTATCGAGAAAGATTTATGGGCAACCGGAACTGTGAACGAGGAGTACCTGAAAGCTCTGAATGCTCTTTTTAGCAACTTCCCACGACTTAGAGCCACAGAACCTGCAACGTTAAGCATTCCTCATCTTGTTACATCCCTCAAGACAGGGTCAGAGGCAACTCAAGAAGCTGCCTTGGATGCACTCTTTCTTCTTAGGCAAGCTTGGTCAGCATGTCCAGCTGAAGTTTCTAGGGCGCAGTCAATTGCTGCAGCCGATGCAATCCCCTTGCTGCAATACTTAATTCAGTCTGGCCCACCTCGGTTTCAGGAGAAGACAGAATTTTTGTTACAATGTTTGCCGGGGACATTGGTGGTGATAATCAAGCGTGGAAATAATATGAAGCAGTCAGTGGGAAATCCTAGTGTTTATTGCAAGATTACACTCGGCAACACTCCACCTAAGCAAACTCAGGTTGTTTCAACTGGACCAAATCCCGAATGGGATGAAAGCTTTTCGTGGTCCTTTGAGAGTCCTCCGAAAGGCCAGAAGCTCCACATATCTTGCAAGAACAAGAGCAAAATGGGAAAGAGTTCATTTGGAAAGGTAACCATCCAGATTGATCGTGTAGTAATGCTGGGAGCAGTTGCCGGAGAATACACTCTGTTGCCGGAAAGCAAAAGTGGGCCGTCACGGAATTTAGAAATAGAGTTCCAGTGGTCTAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0002682 GO:0002683 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005829 GO:0005856 GO:0005874 GO:0005881 GO:0005886 GO:0005911 GO:0005938 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008017 GO:0008092 GO:0008104 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009506 GO:0009555 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009653 GO:0009664 GO:0009791 GO:0009832 GO:0009833 GO:0009856 GO:0009898 GO:0009900 GO:0009901 GO:0009908 GO:0009987 GO:0010035 GO:0010038 GO:0010208 GO:0010215 GO:0010330 GO:0010927 GO:0010941 GO:0012505 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016049 GO:0016051 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0022414 GO:0022603 GO:0022607 GO:0030054 GO:0030198 GO:0030243 GO:0030244 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032886 GO:0032989 GO:0032991 GO:0033036 GO:0033043 GO:0033365 GO:0033612 GO:0033692 GO:0034613 GO:0034637 GO:0034645 GO:0036211 GO:0036449 GO:0040007 GO:0042221 GO:0042546 GO:0042742 GO:0042981 GO:0043062 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044380 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044706 GO:0045229 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048467 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048608 GO:0048609 GO:0048646 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051211 GO:0051239 GO:0051273 GO:0051274 GO:0051301 GO:0051493 GO:0051592 GO:0051641 GO:0051704 GO:0051707 GO:0055028 GO:0055044 GO:0060548 GO:0061458 GO:0061640 GO:0065007 GO:0070507 GO:0070647 GO:0070696 GO:0070726 GO:0070727 GO:0071554 GO:0071555 GO:0071668 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0072697 GO:0072698 GO:0072699 GO:0085029 GO:0090567 GO:0097435 GO:0098542 GO:0098552 GO:0098562 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1902494 GO:1903047 GO:1905421 GO:1990234 GO:1990752 GO:1990778 GO:2000026 GO:2000028 GO:2000067 GO:2000241 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm C2

Protein Analysis

1491

Amino Acids

160.78

Weight (kDa)

5.5

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 1369 - 1463 8.6e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 751, 1618, 3874
AccB1I GGYRCC 1 cut(s) 2524
AccB7I CCANNNNNTGG 1 cut(s) 2654
AccI GTMKAC 2 cut(s) 1813, 2908
AciI CCGC 1 cut(s) 308
AclI AACGTT 2 cut(s) 2697, 3872
AclWI GGATC 6 cut(s) 1301, 3020, 3267, 3383, 3645, 3652
AcoI YGGCCR 2 cut(s) 1494, 2963
AcsI RAATTY 6 cut(s) 764, 840, 1247, 3098, 4082, 4438
AcuI CTGAAG 4 cut(s) 381, 501, 3552, 4005
AcvI CACGTG 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 3621
AfiI CCNNNNNNNGG 9 cut(s) 289, 343, 1554, 2311, 2381, 2521, 2654, 3457, 4283
AflIII ACRYGT 1 cut(s) 425
AhlI ACTAGT 1 cut(s) 3502
AjnI CCWGG 7 cut(s) 197, 529, 710, 1093, 1595, 1648, 3624
Alw21I GWGCWC 7 cut(s) 140, 433, 1408, 1657, 1978, 2680, 3502
Alw26I GTCTC 7 cut(s) 110, 146, 1386, 1834, 2265, 3378, 3733
Alw44I GTGCAC 2 cut(s) 2676, 3498
AlwI GGATC 6 cut(s) 1301, 3020, 3267, 3383, 3645, 3652
AlwNI CAGNNNCTG 5 cut(s) 479, 530, 3062, 3608, 3866
Ama87I CYCGRG 1 cut(s) 357
ApaLI GTGCAC 2 cut(s) 2676, 3498
ApoI RAATTY 6 cut(s) 764, 840, 1247, 3098, 4082, 4438
ArsI GACNNNNNNTTYG 4 cut(s) 1838, 1870, 4069, 4101
Asp700I GAANNNNTTC 3 cut(s) 1903, 2844, 4333
AspLEI GCGC 3 cut(s) 2956, 3218, 4000
AsuC2I CCSGG 3 cut(s) 338, 497, 4104
AsuHPI GGTGA 8 cut(s) 893, 943, 1302, 2491, 3022, 3452, 3763, 4129
AvaI CYCGRG 1 cut(s) 357
AvaII GGWCC 6 cut(s) 64, 2597, 2657, 2879, 4229, 4263
BaeGI GKGCMC 2 cut(s) 2680, 3502
BalI TGGCCA 1 cut(s) 1496
BanI GGYRCC 1 cut(s) 2524
BanII GRGCYC 2 cut(s) 2434, 2625
BarI GAAGNNNNNNTAC 4 cut(s) 510, 542, 3123, 3155
BauI CACGAG 1 cut(s) 2435
BbrPI CACGTG 1 cut(s) 86
BbsI GAAGAC 3 cut(s) 1578, 2378, 4082
Bbv12I GWGCWC 7 cut(s) 140, 433, 1408, 1657, 1978, 2680, 3502
BbvCI CCTCAGC 1 cut(s) 3609
BccI CCATC 9 cut(s) 257, 1486, 1711, 2086, 2464, 2608, 2657, 3037, 4361
BceAI ACGGC 2 cut(s) 866, 4414
BciT130I CCWGG 7 cut(s) 199, 531, 712, 1095, 1597, 1650, 3626
BclI TGATCA 1 cut(s) 933
BcnI CCSGG 3 cut(s) 338, 497, 4104
BcoDI GTCTC 7 cut(s) 110, 146, 1386, 1834, 2265, 3378, 3733
BcuI ACTAGT 1 cut(s) 3502
BfmI CTRYAG 4 cut(s) 1797, 2232, 3083, 4011
BfuAI ACCTGC 3 cut(s) 751, 1618, 3874
BglII AGATCT 4 cut(s) 1021, 1801, 3269, 3587
BlpI GCTNAGC 2 cut(s) 2811, 3405
BmcAI AGTACT 1 cut(s) 1434
Bme18I GGWCC 6 cut(s) 64, 2597, 2657, 2879, 4229, 4263
BmeT110I CYCGRG 1 cut(s) 357
BmiI GGNNCC 5 cut(s) 66, 2020, 2250, 2526, 2598
BpiI GAAGAC 3 cut(s) 1578, 2378, 4082
BpmI CTGGAG 5 cut(s) 750, 984, 1671, 2508, 2772
Bpu10I CCTNAGC 4 cut(s) 1536, 2592, 3609, 4203
Bpu1102I GCTNAGC 2 cut(s) 2811, 3405
BpuEI CTTGAG 6 cut(s) 141, 710, 1571, 1758, 3887, 3909
BpuMI CCSGG 3 cut(s) 338, 497, 4104
Bsa29I ATCGAT 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 86
BsaHI GRCGYC 1 cut(s) 3621
BsaJI CCNNGG 9 cut(s) 60, 1093, 1094, 1995, 2088, 3006, 3936, 4061, 4103
BsaWI WCCGGW 1 cut(s) 3788
Bsc4I CCNNNNNNNGG 9 cut(s) 289, 343, 1554, 2311, 2381, 2521, 2654, 3457, 4283
Bse1I ACTGG 9 cut(s) 967, 1430, 1448, 2020, 2343, 3198, 3426, 4231, 4456
Bse3DI GCAATG 6 cut(s) 222, 798, 1077, 2586, 3231, 3689
BseBI CCWGG 7 cut(s) 199, 531, 712, 1095, 1597, 1650, 3626
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 9 cut(s) 60, 1093, 1094, 1995, 2088, 3006, 3936, 4061, 4103
BseLI CCNNNNNNNGG 9 cut(s) 289, 343, 1554, 2311, 2381, 2521, 2654, 3457, 4283
BseMI GCAATG 6 cut(s) 222, 798, 1077, 2586, 3231, 3689
BseNI ACTGG 9 cut(s) 967, 1430, 1448, 2020, 2343, 3198, 3426, 4231, 4456
BseRI GAGGAG 1 cut(s) 3818
BseSI GKGCMC 2 cut(s) 2680, 3502
BseYI CCCAGC 3 cut(s) 1047, 1265, 4378
BsgI GTGCAG 4 cut(s) 957, 1467, 1596, 2271
BshNI GGYRCC 1 cut(s) 2524
BshVI ATCGAT 1 cut(s) 6
BsiHKAI GWGCWC 7 cut(s) 140, 433, 1408, 1657, 1978, 2680, 3502
BsiHKCI CYCGRG 1 cut(s) 357
BsiSI CCGG 6 cut(s) 337, 497, 3789, 4103, 4392, 4412
BslFI GGGAC 3 cut(s) 50, 2610, 4120
BslI CCNNNNNNNGG 9 cut(s) 289, 343, 1554, 2311, 2381, 2521, 2654, 3457, 4283
BsmAI GTCTC 7 cut(s) 110, 146, 1386, 1834, 2265, 3378, 3733
BsmFI GGGAC 3 cut(s) 50, 2610, 4120
BsmI GAATGC 7 cut(s) 1969, 2119, 2293, 3078, 3184, 3829, 3879
BsoBI CYCGRG 1 cut(s) 357
Bsp1286I GDGCHC 9 cut(s) 140, 433, 1408, 1657, 1978, 2434, 2625, 2680, 3502
Bsp1407I TGTACA 1 cut(s) 3579
Bsp1720I GCTNAGC 2 cut(s) 2811, 3405
BspACI CCGC 1 cut(s) 308
BspDI ATCGAT 1 cut(s) 6
BspHI TCATGA 2 cut(s) 661, 3040
BspLI GGNNCC 5 cut(s) 66, 2020, 2250, 2526, 2598
BspMAI CTGCAG 3 cut(s) 2236, 3087, 4015
BspMI ACCTGC 3 cut(s) 751, 1618, 3874
BspPI GGATC 6 cut(s) 1301, 3020, 3267, 3383, 3645, 3652
BspQI GCTCTTC 3 cut(s) 1894, 3189, 3513
BspT107I GGYRCC 1 cut(s) 2524
BsrDI GCAATG 6 cut(s) 222, 798, 1077, 2586, 3231, 3689
BsrGI TGTACA 1 cut(s) 3579
BsrI ACTGG 9 cut(s) 967, 1430, 1448, 2020, 2343, 3198, 3426, 4231, 4456
BssECI CCNNGG 9 cut(s) 60, 1093, 1094, 1995, 2088, 3006, 3936, 4061, 4103
BssNAI GTATAC 2 cut(s) 1814, 2909
BssNI GRCGYC 1 cut(s) 3621
BssSI CACGAG 1 cut(s) 2435
BssT1I CCWWGG 4 cut(s) 1995, 2088, 3006, 3936
Bst1107I GTATAC 2 cut(s) 1814, 2909
Bst2BI CACGAG 1 cut(s) 2435
Bst2UI CCWGG 7 cut(s) 199, 531, 712, 1095, 1597, 1650, 3626
Bst6I CTCTTC 9 cut(s) 144, 400, 1506, 1855, 1894, 2268, 3039, 3189, 3513
BstACI GRCGYC 1 cut(s) 3621
BstAPI GCANNNNNTGC 9 cut(s) 353, 428, 557, 1610, 1973, 2048, 2588, 3179, 4019
BstAUI TGTACA 1 cut(s) 3579
BstBAI YACGTR 1 cut(s) 86
BstDSI CCRYGG 1 cut(s) 60
BstEII GGTNACC 1 cut(s) 4348
BstENI CCTNNNNNAGG 4 cut(s) 287, 2379, 2519, 4281
BstHHI GCGC 3 cut(s) 2956, 3218, 4000
BstMAI GTCTC 7 cut(s) 110, 146, 1386, 1834, 2265, 3378, 3733
BstNI CCWGG 7 cut(s) 199, 531, 712, 1095, 1597, 1650, 3626
BstNSI RCATGY 5 cut(s) 429, 1136, 1412, 2039, 3978
BstPI GGTNACC 1 cut(s) 4348
BstSFI CTRYAG 4 cut(s) 1797, 2232, 3083, 4011
BstSLI GKGCMC 2 cut(s) 2680, 3502
BstV2I GAAGAC 3 cut(s) 1578, 2378, 4082
BstX2I RGATCY 9 cut(s) 1021, 1293, 1801, 3025, 3259, 3269, 3388, 3587, 3637
BstYI RGATCY 9 cut(s) 1021, 1293, 1801, 3025, 3259, 3269, 3388, 3587, 3637
BstZ17I GTATAC 2 cut(s) 1814, 2909
Bsu15I ATCGAT 1 cut(s) 6
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 60
BtgZI GCGATG 1 cut(s) 1505
BtsI GCAGTG 1 cut(s) 1831
BveI ACCTGC 3 cut(s) 751, 1618, 3874
CaiI CAGNNNCTG 5 cut(s) 479, 530, 3062, 3608, 3866
CciI TCATGA 2 cut(s) 661, 3040
CfoI GCGC 3 cut(s) 2956, 3218, 4000
ClaI ATCGAT 1 cut(s) 6
CseI GACGC 2 cut(s) 3488, 3610
CspCI CAANNNNNGTGG 2 cut(s) 4286, 4321
DraI TTTAAA 2 cut(s) 45, 1333
EaeI YGGCCR 2 cut(s) 1494, 2963
Eam1104I CTCTTC 9 cut(s) 144, 400, 1506, 1855, 1894, 2268, 3039, 3189, 3513
EarI CTCTTC 9 cut(s) 144, 400, 1506, 1855, 1894, 2268, 3039, 3189, 3513
Eco130I CCWWGG 4 cut(s) 1995, 2088, 3006, 3936
Eco147I AGGCCT 2 cut(s) 293, 2458
Eco24I GRGCYC 2 cut(s) 2434, 2625
Eco47I GGWCC 6 cut(s) 64, 2597, 2657, 2879, 4229, 4263
Eco57I CTGAAG 4 cut(s) 381, 501, 3552, 4005
Eco72I CACGTG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 357
Eco91I GGTNACC 1 cut(s) 4348
EcoNI CCTNNNNNAGG 4 cut(s) 287, 2379, 2519, 4281
EcoO109I RGGNCCY 1 cut(s) 3460
EcoO65I GGTNACC 1 cut(s) 4348
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 7 cut(s) 197, 529, 710, 1093, 1595, 1648, 3624
EcoT14I CCWWGG 4 cut(s) 1995, 2088, 3006, 3936
EcoT38I GRGCYC 2 cut(s) 2434, 2625
ErhI CCWWGG 4 cut(s) 1995, 2088, 3006, 3936
FalI AAGNNNNNCTT 8 cut(s) 1533, 1565, 2390, 2422, 2829, 2861, 3226, 3258
FaqI GGGAC 3 cut(s) 50, 2610, 4120
FauNDI CATATG 1 cut(s) 1349
FbaI TGATCA 1 cut(s) 933
FblI GTMKAC 2 cut(s) 1813, 2908
FriOI GRGCYC 2 cut(s) 2434, 2625
GlaI GCGC 3 cut(s) 2955, 3217, 3999
GsaI CCCAGC 3 cut(s) 1051, 1269, 4382
GsuI CTGGAG 5 cut(s) 750, 984, 1671, 2508, 2772
HapII CCGG 6 cut(s) 337, 497, 3789, 4103, 4392, 4412
HgaI GACGC 2 cut(s) 3488, 3610
HhaI GCGC 3 cut(s) 2956, 3218, 4000
Hin1I GRCGYC 1 cut(s) 3621
Hin6I GCGC 3 cut(s) 2954, 3216, 3998
HinP1I GCGC 3 cut(s) 2954, 3216, 3998
HincII GTYRAC 3 cut(s) 2223, 2695, 2736
HindII GTYRAC 3 cut(s) 2223, 2695, 2736
HindIII AAGCTT 3 cut(s) 1936, 3963, 4252
HpaII CCGG 6 cut(s) 337, 497, 3789, 4103, 4392, 4412
HphI GGTGA 8 cut(s) 893, 943, 1302, 2491, 3022, 3452, 3763, 4129
Hpy166II GTNNAC 8 cut(s) 1814, 2223, 2678, 2695, 2736, 2909, 3500, 3799
Hpy8I GTNNAC 8 cut(s) 1814, 2223, 2678, 2695, 2736, 2909, 3500, 3799
HpyAV CCTTC 8 cut(s) 83, 196, 512, 713, 2020, 2929, 3149, 3535
HpyCH4IV ACGT 5 cut(s) 85, 298, 1528, 2697, 3872
HpySE526I ACGT 5 cut(s) 85, 298, 1528, 2697, 3872
Hsp92I GRCGYC 1 cut(s) 3621
HspAI GCGC 3 cut(s) 2954, 3216, 3998
Ksp22I TGATCA 1 cut(s) 933
LguI GCTCTTC 3 cut(s) 1894, 3189, 3513
MaeII ACGT 5 cut(s) 85, 298, 1528, 2697, 3872
MfeI CAATTG 5 cut(s) 111, 555, 918, 2043, 4005
MflI RGATCY 9 cut(s) 1021, 1293, 1801, 3025, 3259, 3269, 3388, 3587, 3637
MhlI GDGCHC 9 cut(s) 140, 433, 1408, 1657, 1978, 2434, 2625, 2680, 3502
MlsI TGGCCA 1 cut(s) 1496
MluNI TGGCCA 1 cut(s) 1496
MlyI GAGTC 6 cut(s) 148, 1169, 1675, 1841, 2857, 4282
MmeI TCCRAC 3 cut(s) 2292, 3285, 3615
Mox20I TGGCCA 1 cut(s) 1496
MroXI GAANNNNTTC 3 cut(s) 1903, 2844, 4333
MscI TGGCCA 1 cut(s) 1496
MslI CAYNNNNRTG 1 cut(s) 3279
Msp20I TGGCCA 1 cut(s) 1496
MspA1I CMGCKG 3 cut(s) 350, 3059, 3983
MspI CCGG 6 cut(s) 337, 497, 3789, 4103, 4392, 4412
MunI CAATTG 5 cut(s) 111, 555, 918, 2043, 4005
Mva1269I GAATGC 7 cut(s) 1969, 2119, 2293, 3078, 3184, 3829, 3879
MvaI CCWGG 7 cut(s) 199, 531, 712, 1095, 1597, 1650, 3626
NciI CCSGG 3 cut(s) 338, 497, 4104
NdeI CATATG 1 cut(s) 1349
NlaIV GGNNCC 5 cut(s) 66, 2020, 2250, 2526, 2598
NmeAIII GCCGAG 2 cut(s) 2206, 4167
NmuCI GTSAC 7 cut(s) 881, 1229, 2269, 2820, 2860, 2992, 4431
NspI RCATGY 5 cut(s) 429, 1136, 1412, 2039, 3978
PaeI GCATGC 1 cut(s) 1136
PaeR7I CTCGAG 1 cut(s) 357
PagI TCATGA 2 cut(s) 661, 3040
PasI CCCWGGG 1 cut(s) 1094
PceI AGGCCT 2 cut(s) 293, 2458
PciI ACATGT 1 cut(s) 425
PciSI GCTCTTC 3 cut(s) 1894, 3189, 3513
PctI GAATGC 7 cut(s) 1969, 2119, 2293, 3078, 3184, 3829, 3879
PdmI GAANNNNTTC 3 cut(s) 1903, 2844, 4333
PflFI GACNNNGTC 1 cut(s) 3910
PflMI CCANNNNNTGG 1 cut(s) 2654
PfoI TCCNGGA 3 cut(s) 197, 1595, 1648
PleI GAGTC 6 cut(s) 148, 1168, 1675, 1841, 2857, 4281
PmaCI CACGTG 1 cut(s) 86
PmlI CACGTG 1 cut(s) 86
PpsI GAGTC 6 cut(s) 148, 1168, 1675, 1841, 2857, 4281
Ppu21I YACGTR 1 cut(s) 86
PscI ACATGT 1 cut(s) 425
Psp1406I AACGTT 2 cut(s) 2697, 3872
Psp6I CCWGG 7 cut(s) 197, 529, 710, 1093, 1595, 1648, 3624
PspCI CACGTG 1 cut(s) 86
PspEI GGTNACC 1 cut(s) 4348
PspFI CCCAGC 3 cut(s) 1047, 1265, 4378
PspGI CCWGG 7 cut(s) 197, 529, 710, 1093, 1595, 1648, 3624
PspN4I GGNNCC 5 cut(s) 66, 2020, 2250, 2526, 2598
PsrI GAACNNNNNNTAC 2 cut(s) 3791, 3823
PstI CTGCAG 3 cut(s) 2236, 3087, 4015
PstNI CAGNNNCTG 5 cut(s) 479, 530, 3062, 3608, 3866
PsuI RGATCY 9 cut(s) 1021, 1293, 1801, 3025, 3259, 3269, 3388, 3587, 3637
PsyI GACNNNGTC 1 cut(s) 3910
PvuII CAGCTG 3 cut(s) 350, 3059, 3983
RseI CAYNNNNRTG 1 cut(s) 3279
SapI GCTCTTC 3 cut(s) 1894, 3189, 3513
ScaI AGTACT 1 cut(s) 1434
SchI GAGTC 6 cut(s) 148, 1169, 1675, 1841, 2857, 4282
SduI GDGCHC 9 cut(s) 140, 433, 1408, 1657, 1978, 2434, 2625, 2680, 3502
SfcI CTRYAG 4 cut(s) 1797, 2232, 3083, 4011
Sfr274I CTCGAG 1 cut(s) 357
SinI GGWCC 6 cut(s) 64, 2597, 2657, 2879, 4229, 4263
SlaI CTCGAG 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 3279
SmlI CTYRAG 7 cut(s) 156, 357, 689, 1550, 1773, 3902, 3924
SmoI CTYRAG 7 cut(s) 156, 357, 689, 1550, 1773, 3902, 3924
SpeI ACTAGT 1 cut(s) 3502
SphI GCATGC 1 cut(s) 1136
SseBI AGGCCT 2 cut(s) 293, 2458
SsiI CCGC 1 cut(s) 308
SspI AATATT 4 cut(s) 132, 189, 315, 2568
StuI AGGCCT 2 cut(s) 293, 2458
StyI CCWWGG 4 cut(s) 1995, 2088, 3006, 3936
TaiI ACGT 5 cut(s) 88, 301, 1531, 2700, 3875
TaqI TCGA 8 cut(s) 6, 267, 358, 1706, 1978, 3469, 3656, 3768
TatI WGTACW 4 cut(s) 1358, 1432, 2794, 3579
TauI GCSGC 1 cut(s) 311
TseFI GTSAC 7 cut(s) 881, 1229, 2269, 2820, 2860, 2992, 4431
Tsp45I GTSAC 7 cut(s) 881, 1229, 2269, 2820, 2860, 2992, 4431
TspGWI ACGGA 3 cut(s) 49, 2294, 4450
Tth111I GACNNNGTC 1 cut(s) 3910
Van91I CCANNNNNTGG 1 cut(s) 2654
VneI GTGCAC 2 cut(s) 2676, 3498
VpaK11BI GGWCC 6 cut(s) 64, 2597, 2657, 2879, 4229, 4263
XagI CCTNNNNNAGG 4 cut(s) 287, 2379, 2519, 4281
XapI RAATTY 6 cut(s) 764, 840, 1247, 3098, 4082, 4438
XbaI TCTAGA 2 cut(s) 646, 2426
XceI RCATGY 5 cut(s) 429, 1136, 1412, 2039, 3978
XcmI CCANNNNNNNNNTGG 1 cut(s) 4239
XhoI CTCGAG 1 cut(s) 357
XmiI GTMKAC 2 cut(s) 1813, 2908
XmnI GAANNNNTTC 3 cut(s) 1903, 2844, 4333
ZrmI AGTACT 1 cut(s) 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.