pycom01g13150

Pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007939
Physical Location & Seq
Forward (+)
13711936 .. 13717695
5760 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3039 bp
ATGCTGTCAACCCTAGATCGATCCAATGGGAATTTAGAAGATGCTTTACAGAGTGAGCTTGAATTGATTCTGAGAGAGCAGCGCAATCAGCATGCCATTCACCGGGAGAGGGACGTGAATATGTATAGGAGCGGCAGTGCCCCGCCAACTGTTGAGGGTTCGCTAACTGCTGTGGGTAGTCTCTTTAGGAACTCAGATTTTAGGGATGTGACTAGTAGGATTAGTAATAACAATGGGGTTTTGTCCGAAGATGAGATTCGATCTCATCCTGCTTATCTTTCGTACTACTATTCGCATGATAATATTAACCCAAGATTGCCTCCACCGTTGTTGTCGAGAGAGGATTGGCGTGTTGCACAAAGGTTTCAGGGTGGCGGGTCTTCTTTTGAGGGCAATGGGGACTGGAGGAGGAAGAAGTTGGCTGATGATGGTGATAGTTTGTCGCTGTTTTCCACGCAGTTGGGACTACAAAAGGCAGAGGATGAGTTGATGGGACTGAGGAATGCTAAAGGTAGCAATCTCCGTAAACAGACTTCATCTGAGTGGCTTGATAAAAGCACGGATGGTTTGATTGGTTTGTCGACAACTGGGTTTGGTGGAAGGAGAAAGAGTTTTGCTGACATTTTACAGGAAGGACTTGATGAAACTGCATCCTTGTCAGGTCCCCTGTCAAGGCCATCAAGTCGTAACGCTTTTGGTGATTTCATGGATTCCACAGGCATGGCTGATCCCTATCCAGTTGGGTTAACTAATGGAGTGGAGTCTGCAGAGGGTATGCATAATGGACCAGCTTCACGTGGCCTGGTGGGAGTTCAGAGCCATGGCCTAACAACTTCTCATTCCTTTGCATCTGCTGTGGGTCCTTCTTTGCCAAGAAGCAGAACCCCTGAACAACTATTTGGGAGGTCCACCGGTTCTGGTCTCCGCCCTGTAGGGAGTAGAGTATTCCCTACCGAGAGGAAGAATGTTGTTAGTCCAGATATTCAAAATGATCGATCTTCCAGCATGAATGATCTGGCGGATATTTCAGCCAATTTTTCTGGCTTAAGCCTATCAAAAATTAGACCTGTAGATGATGATAGACGTATTCAGTCGCAGCTTCATGTGGATCTTGATAATCAGCGTGATTTTCTGTTTAAGATGCCAAATGGGCATCAGAGGCTGCAGCAGGAACTGTTAGAGAAGGCAAATGCTGAAATTTTTTCTCTTCCTACTAATTATGCTCATCTAGCAAAGAAAAATGGAATCATAACAAACCGTACTACATCCAATGGGCAAGCTAATTTTTCAAGAAGAACTTCTTCTCCCAGTCTGTACTCAAAAGGGAGTTCTTCAGGCTTTGGAGCTTTGGAAGGATCTAATGTTCATTACCAAGAAGCAAATCCAGCAGGTATGGACTTTGATGGCCATGTTGCCGGGGCCTATCCTGTAAATCCAAAGTTGAACATGACAAGTGATAATCATCTTGGTGCAGTAGCTAGTGGTGATGAACAAAGTCTGAACAGACTGGGAAACAAAGTAGGGCCTGGTCTTCATTCTTCTGTTATGGACCCATCTTATATTCAGTTTTTGCAAAGAACTGATTACGCAGCACTTAATGTAACTAGTCCAAGTGGTTATCCTCTGAGCAAAAACCACTTTGGCACTTTGCATGGAGACTTAGAGGGGCTTCAGAAGGCATATCTTGAGGCATTGCTTGCTCAAAGGAAGCAACAGTATGAGTTGTCAGTTTTAGGTAACTCTGACGGTTTTAATCATGGATATTATGGGAACCCACCATATGGGCTTGGCATGTATCCAGGAAATCCAATGGCAAATTCACTTCATCCTTCTATTGGGTATGGTAGTCCAATGTTTGAGAGTGAGAAGATTGCCCACTTTAATTCTATGTTGAGAAGTTCAATGGGAGGGTCGGTTAGCTCCTGGAACTCAGATATTGGAAACAACCTAGAAGGACGACATGCATCATCATTATTAGATGAATTCAAGAACAATAAGAACAAATCTTTTGAATTTGCAGACATTGTTGATCACGTAATTGAATTCAGTACCGACCAGTATGGAAGTCGCTTCATTCAGCAGAAACTAGAAACAGCCACTGTGGAAGAAAAAATGAATATATTTCCAGAGATTATTCCTCATGCTCGCGCGTTGATGACTGATGTATTCGGAAATTATGTCGTACAGAAATTTTTTGAGCATGGTACAGAAAGCCAAAGAAAAGAGTTAACCAGCCAACTTACTGGCCATGTTTTGCCTCTCAGTCTTCAAATGTATGGTTGTAGAGTGATTCAGAAGGCCTTGGAAGTGGTTGATGTGAATCAGCAGGCACAGATGGTGTTGGAGCTTGATGGTTCAATCATGAAATGCGTCCGTGATCAGAATGGGAATCATGTCATTCAGAAGTGTATAGAATGTATCCCTCAAGATAAAATTCAATTTATAATTTCATCCTTCTATGGGCAAGTTGTCACACTATCCACTCATCCTTATGGATGCCGTGTTATACAGAGGGTTCTGGAACATTGCAATGATTCAAACACACAACAAATAATCATGGATGAAATCATGCAATCTGTATGCATTTTGGCACAATCGGAGATCGATCAATATGGAAATTACGTCATTCAGCATGTCCTTGAACATGGTAAACCGCACGAACGGACTGCAATTATCCACAAGCTTGCAGGACAAATTGTGAAGATGAGTCAGCAGAAGTTTGCTTCTAATGTTGTGGAGAAGTGCTTAACTTTTGGGAATGCTGATGAGCGCCAGTTTTTGGTGAATGAGATGCTGGGTTCAACTGATGAAAATGAGCCCTTACAGGCCATGATGAAAGATCCCTTTGGAAACTACGTTGTGCAGAAGGTTCTTGAGACTTGTGATGATCAGAGTCTTGAGTTGATTCTTTCTCGCATTAAGGTTCATTTGACTGCCCTGAAGAAGTATACCTACGGCAAACATATCGTTTCTCGTGTCGAGAAACTCATCACAACTGGAGAAAGGCGCATAGGGTTGTCAGCCTCGTGTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

1013

Amino Acids

111.89

Weight (kDa)

6.14

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 302 - 672 4.9e-110 Nucleic acid binding protein NABP
PUF PF00806 678 - 712 1.3e-10 Pumilio-family RNA binding repeat
PUF PF00806 717 - 745 6e-08 Pumilio-family RNA binding repeat
PUF PF00806 750 - 783 7.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 822 - 857 7.9e-14 Pumilio-family RNA binding repeat
PUF PF00806 869 - 895 2.8e-07 Pumilio-family RNA binding repeat
PUF PF00806 898 - 932 7.9e-08 Pumilio-family RNA binding repeat
PUF PF00806 944 - 973 6.1e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2444
Acc36I ACCTGC 1 cut(s) 1379
AccB7I CCANNNNNTGG 2 cut(s) 1781, 2779
AccBSI CCGCTC 1 cut(s) 132
AccI GTMKAC 2 cut(s) 581, 2948
AccII CGCG 2 cut(s) 2148, 2150
AciI CCGC 6 cut(s) 132, 143, 375, 925, 1019, 2654
AclWI GGATC 5 cut(s) 15, 722, 1116, 1363, 2834
AcoI YGGCCR 2 cut(s) 1405, 2245
AcsI RAATTY 8 cut(s) 31, 1197, 1816, 1982, 2012, 2042, 2189, 2433
AcuI CTGAAG 3 cut(s) 1317, 1655, 2960
AcvI CACGTG 1 cut(s) 797
AfaI GTAC 6 cut(s) 284, 1261, 1316, 2050, 2184, 2206
AfiI CCNNNNNNNGG 7 cut(s) 102, 109, 672, 1781, 1835, 2460, 2779
AflII CTTAAG 1 cut(s) 1045
AgeI ACCGGT 1 cut(s) 911
AhlI ACTAGT 2 cut(s) 212, 1604
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 4 cut(s) 801, 1525, 1798, 1922
AjuI GAANNNNNNNTTGG 4 cut(s) 882, 914, 1801, 1833
AloI GAACNNNNNNTCC 4 cut(s) 1288, 1320, 1347, 1379
AluBI AGCT 9 cut(s) 58, 791, 1099, 1280, 1346, 1478, 1920, 2347, 2683
AluI AGCT 9 cut(s) 58, 791, 1099, 1280, 1346, 1478, 1920, 2347, 2683
Alw26I GTCTC 4 cut(s) 185, 926, 1650, 2870
AlwI GGATC 5 cut(s) 15, 722, 1116, 1363, 2834
AlwNI CAGNNNCTG 3 cut(s) 1162, 1174, 2099
AoxI GGCC 9 cut(s) 674, 799, 823, 1405, 1419, 1523, 2245, 2298, 2826
ApeKI GCWGC 5 cut(s) 79, 1096, 1162, 1165, 1589
ApoI RAATTY 8 cut(s) 31, 1197, 1816, 1982, 2012, 2042, 2189, 2433
AsiGI ACCGGT 1 cut(s) 911
Asp700I GAANNNNTTC 3 cut(s) 66, 1297, 1300
AspLEI GCGC 4 cut(s) 84, 2150, 2772, 3009
AspS9I GGNCC 7 cut(s) 662, 785, 860, 906, 1419, 1523, 1549
AsuC2I CCSGG 2 cut(s) 104, 1417
AsuHPI GGTGA 5 cut(s) 92, 443, 710, 1496, 2794
AvaII GGWCC 5 cut(s) 662, 785, 860, 906, 1549
BaeGI GKGCMC 1 cut(s) 142
BalI TGGCCA 2 cut(s) 1407, 2247
BanII GRGCYC 1 cut(s) 2820
BarI GAAGNNNNNNTAC 6 cut(s) 30, 62, 517, 549, 2936, 2968
BauI CACGAG 2 cut(s) 2973, 3025
BbrPI CACGTG 1 cut(s) 797
BbsI GAAGAC 3 cut(s) 372, 1523, 2258
BbvI GCAGC 5 cut(s) 91, 1108, 1149, 1177, 1601
BccI CCATC 8 cut(s) 422, 484, 557, 685, 1397, 1561, 2329, 2345
BceAI ACGGC 2 cut(s) 2484, 2971
BcgI CGANNNNNNTGC 4 cut(s) 2648, 2682, 2947, 2981
BciT130I CCWGG 4 cut(s) 803, 1527, 1800, 1924
BciVI GTATCC 2 cut(s) 1806, 2429
BclI TGATCA 3 cut(s) 2029, 2377, 2887
BcnI CCSGG 2 cut(s) 104, 1417
BcoDI GTCTC 4 cut(s) 185, 926, 1650, 2870
BcuI ACTAGT 2 cut(s) 212, 1604
BfaI CTAG 7 cut(s) 14, 213, 1229, 1479, 1605, 1949, 2087
BfmI CTRYAG 4 cut(s) 765, 930, 1068, 1163
BfoI RGCGCY 1 cut(s) 2773
BfrI CTTAAG 1 cut(s) 1045
BfuAI ACCTGC 1 cut(s) 1379
BfuI GTATCC 2 cut(s) 1806, 2429
BglI GCCNNNNNGGC 1 cut(s) 1150
BisI GCNGC 6 cut(s) 80, 133, 1097, 1163, 1166, 1590
BlsI GCNGC 6 cut(s) 81, 134, 1098, 1164, 1167, 1591
Bme1390I CCNGG 6 cut(s) 104, 803, 1417, 1527, 1800, 1924
Bme18I GGWCC 5 cut(s) 662, 785, 860, 906, 1549
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 7 cut(s) 662, 785, 860, 906, 1419, 1523, 1549
BmiI GGNNCC 5 cut(s) 664, 861, 1420, 1551, 1772
BmrFI CCNGG 6 cut(s) 104, 803, 1417, 1527, 1800, 1924
BmrI ACTGGG 3 cut(s) 597, 1302, 1517
BmsI GCATC 8 cut(s) 31, 659, 857, 1131, 1162, 1973, 2486, 2781
BmuI ACTGGG 3 cut(s) 597, 1302, 1517
BpiI GAAGAC 3 cut(s) 372, 1523, 2258
BpmI CTGGAG 2 cut(s) 424, 3018
BpuEI CTTGAG 4 cut(s) 1706, 2409, 2894, 2918
BpuMI CCSGG 2 cut(s) 104, 1417
Bsa29I ATCGAT 3 cut(s) 19, 994, 2604
BsaAI YACGTR 2 cut(s) 797, 2035
BsaBI GATNNNNATC 3 cut(s) 732, 1461, 2319
BsaI GGTCTC 1 cut(s) 926
BsaJI CCNNGG 3 cut(s) 820, 1416, 2301
BsaWI WCCGGW 1 cut(s) 911
BsaXI ACNNNNNCTCC 8 cut(s) 121, 151, 595, 625, 1288, 1318, 1899, 1929
Bsc4I CCNNNNNNNGG 7 cut(s) 102, 109, 672, 1781, 1835, 2460, 2779
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 9 cut(s) 407, 592, 737, 1308, 1512, 2056, 2248, 2773, 3001
Bse3DI GCAATG 4 cut(s) 400, 1691, 2524, 2536
Bse8I GATNNNNATC 3 cut(s) 732, 1461, 2319
BseBI CCWGG 4 cut(s) 803, 1527, 1800, 1924
BseCI ATCGAT 3 cut(s) 19, 994, 2604
BseDI CCNNGG 3 cut(s) 820, 1416, 2301
BseJI GATNNNNATC 3 cut(s) 732, 1461, 2319
BseLI CCNNNNNNNGG 7 cut(s) 102, 109, 672, 1781, 1835, 2460, 2779
BseMI GCAATG 4 cut(s) 400, 1691, 2524, 2536
BseMII CTCAG 7 cut(s) 62, 207, 488, 531, 1616, 1944, 2275
BseNI ACTGG 9 cut(s) 407, 592, 737, 1308, 1512, 2056, 2248, 2773, 3001
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 5 cut(s) 91, 1108, 1149, 1177, 1601
BseYI CCCAGC 1 cut(s) 2794
BsgI GTGCAG 2 cut(s) 1491, 2882
Bsh1236I CGCG 2 cut(s) 2148, 2150
BshFI GGCC 9 cut(s) 676, 801, 825, 1407, 1421, 1525, 2247, 2300, 2828
BshTI ACCGGT 1 cut(s) 911
BshVI ATCGAT 3 cut(s) 19, 994, 2604
BsiSI CCGG 3 cut(s) 103, 912, 1416
BslFI GGGAC 5 cut(s) 125, 413, 477, 507, 648
BslI CCNNNNNNNGG 7 cut(s) 102, 109, 672, 1781, 1835, 2460, 2779
BsmAI GTCTC 4 cut(s) 185, 926, 1650, 2870
BsmFI GGGAC 5 cut(s) 125, 413, 477, 507, 648
BsmI GAATGC 2 cut(s) 508, 2764
BsnI GGCC 9 cut(s) 676, 801, 825, 1407, 1421, 1525, 2247, 2300, 2828
Bso31I GGTCTC 1 cut(s) 926
Bsp1286I GDGCHC 2 cut(s) 142, 2820
Bsp19I CCATGG 1 cut(s) 820
BspACI CCGC 6 cut(s) 132, 143, 375, 925, 1019, 2654
BspANI GGCC 9 cut(s) 676, 801, 825, 1407, 1421, 1525, 2247, 2300, 2828
BspCNI CTCAG 7 cut(s) 63, 206, 489, 532, 1617, 1943, 2274
BspDI ATCGAT 3 cut(s) 19, 994, 2604
BspFNI CGCG 2 cut(s) 2148, 2150
BspHI TCATGA 1 cut(s) 2361
BspLI GGNNCC 5 cut(s) 664, 861, 1420, 1551, 1772
BspMAI CTGCAG 2 cut(s) 769, 1167
BspMI ACCTGC 1 cut(s) 1379
BspPI GGATC 5 cut(s) 15, 722, 1116, 1363, 2834
BspTI CTTAAG 1 cut(s) 1045
BspTNI GGTCTC 1 cut(s) 926
BsrBI CCGCTC 1 cut(s) 132
BsrDI GCAATG 4 cut(s) 400, 1691, 2524, 2536
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 9 cut(s) 407, 592, 737, 1308, 1512, 2056, 2248, 2773, 3001
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 3 cut(s) 820, 1416, 2301
BssNAI GTATAC 1 cut(s) 2949
BssSI CACGAG 2 cut(s) 2973, 3025
BssT1I CCWWGG 2 cut(s) 820, 2301
Bst1107I GTATAC 1 cut(s) 2949
Bst2BI CACGAG 2 cut(s) 2973, 3025
Bst2UI CCWGG 4 cut(s) 803, 1527, 1800, 1924
Bst4CI ACNGT 7 cut(s) 151, 327, 1176, 1259, 1716, 1748, 2101
Bst6I CTCTTC 1 cut(s) 1212
BstAFI CTTAAG 1 cut(s) 1045
BstAPI GCANNNNNTGC 1 cut(s) 1697
BstBAI YACGTR 2 cut(s) 797, 2035
BstC8I GCNNGC 6 cut(s) 93, 1278, 1698, 2146, 2328, 2685
BstDEI CTNAG 8 cut(s) 71, 193, 497, 540, 1625, 1660, 1930, 2261
BstDSI CCRYGG 1 cut(s) 820
BstFNI CGCG 2 cut(s) 2148, 2150
BstH2I RGCGCY 1 cut(s) 2773
BstHHI GCGC 4 cut(s) 84, 2150, 2772, 3009
BstMAI GTCTC 4 cut(s) 185, 926, 1650, 2870
BstMWI GCNNNNNNNGC 6 cut(s) 88, 1150, 1159, 1229, 1385, 1697
BstNI CCWGG 4 cut(s) 803, 1527, 1800, 1924
BstNSI RCATGY 4 cut(s) 95, 1795, 1964, 2636
BstSCI CCNGG 6 cut(s) 102, 801, 1415, 1525, 1798, 1922
BstSFI CTRYAG 4 cut(s) 765, 930, 1068, 1163
BstSLI GKGCMC 1 cut(s) 142
BstUI CGCG 2 cut(s) 2148, 2150
BstV1I GCAGC 5 cut(s) 91, 1108, 1149, 1177, 1601
BstV2I GAAGAC 3 cut(s) 372, 1523, 2258
BstX2I RGATCY 3 cut(s) 1108, 1355, 2839
BstXI CCANNNNNNTGG 3 cut(s) 460, 721, 2243
BstYI RGATCY 3 cut(s) 1108, 1355, 2839
BstZ17I GTATAC 1 cut(s) 2949
Bsu15I ATCGAT 3 cut(s) 19, 994, 2604
BsuI GTATCC 2 cut(s) 1806, 2429
BsuRI GGCC 9 cut(s) 676, 801, 825, 1407, 1421, 1525, 2247, 2300, 2828
BsuTUI ATCGAT 3 cut(s) 19, 994, 2604
BtgI CCRYGG 1 cut(s) 820
BtrI CACGTC 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 142
BtsIMutI CAGTG 2 cut(s) 142, 2097
BveI ACCTGC 1 cut(s) 1379
Cac8I GCNNGC 6 cut(s) 93, 1278, 1698, 2146, 2328, 2685
CaiI CAGNNNCTG 3 cut(s) 1162, 1174, 2099
CciI TCATGA 1 cut(s) 2361
CfoI GCGC 4 cut(s) 84, 2150, 2772, 3009
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 7 cut(s) 662, 785, 860, 906, 1419, 1523, 1549
ClaI ATCGAT 3 cut(s) 19, 994, 2604
CseI GACGC 1 cut(s) 2359
Csp6I GTAC 6 cut(s) 283, 1260, 1315, 2049, 2183, 2205
CspAI ACCGGT 1 cut(s) 911
CspCI CAANNNNNGTGG 2 cut(s) 312, 347
CviQI GTAC 6 cut(s) 283, 1260, 1315, 2049, 2183, 2205
DdeI CTNAG 8 cut(s) 71, 193, 497, 540, 1625, 1660, 1930, 2261
EaeI YGGCCR 2 cut(s) 1405, 2245
Eam1104I CTCTTC 1 cut(s) 1212
EarI CTCTTC 1 cut(s) 1212
EciI GGCGGA 2 cut(s) 914, 1034
Eco130I CCWWGG 2 cut(s) 820, 2301
Eco147I AGGCCT 1 cut(s) 2300
Eco24I GRGCYC 1 cut(s) 2820
Eco31I GGTCTC 1 cut(s) 926
Eco47I GGWCC 5 cut(s) 662, 785, 860, 906, 1549
Eco57I CTGAAG 3 cut(s) 1317, 1655, 2960
Eco72I CACGTG 1 cut(s) 797
EcoO109I RGGNCCY 4 cut(s) 662, 860, 1419, 1523
EcoRI GAATTC 2 cut(s) 1982, 2042
EcoRII CCWGG 4 cut(s) 801, 1525, 1798, 1922
EcoT14I CCWWGG 2 cut(s) 820, 2301
EcoT22I ATGCAT 3 cut(s) 780, 1966, 2585
EcoT38I GRGCYC 1 cut(s) 2820
ErhI CCWWGG 2 cut(s) 820, 2301
FalI AAGNNNNNCTT 4 cut(s) 1282, 1314, 1285, 1317
FaqI GGGAC 5 cut(s) 125, 413, 477, 507, 648
FauI CCCGC 2 cut(s) 150, 368
FauNDI CATATG 1 cut(s) 1780
FbaI TGATCA 3 cut(s) 2029, 2377, 2887
FblI GTMKAC 2 cut(s) 581, 2948
Fnu4HI GCNGC 6 cut(s) 80, 133, 1097, 1163, 1166, 1590
FriOI GRGCYC 1 cut(s) 2820
Fsp4HI GCNGC 6 cut(s) 80, 133, 1097, 1163, 1166, 1590
FspBI CTAG 7 cut(s) 14, 213, 1229, 1479, 1605, 1949, 2087
GlaI GCGC 4 cut(s) 83, 2149, 2771, 3008
GluI GCNGC 6 cut(s) 80, 133, 1097, 1163, 1166, 1590
GsaI CCCAGC 1 cut(s) 2798
GsuI CTGGAG 2 cut(s) 424, 3018
HaeII RGCGCY 1 cut(s) 2773
HaeIII GGCC 9 cut(s) 676, 801, 825, 1407, 1421, 1525, 2247, 2300, 2828
HapII CCGG 3 cut(s) 103, 912, 1416
HgaI GACGC 1 cut(s) 2359
HhaI GCGC 4 cut(s) 84, 2150, 2772, 3009
Hin6I GCGC 4 cut(s) 82, 2148, 2770, 3007
HinP1I GCGC 4 cut(s) 82, 2148, 2770, 3007
HincII GTYRAC 4 cut(s) 9, 582, 747, 2229
HindII GTYRAC 4 cut(s) 9, 582, 747, 2229
HindIII AAGCTT 1 cut(s) 2681
HpaI GTTAAC 2 cut(s) 747, 2229
HpaII CCGG 3 cut(s) 103, 912, 1416
HphI GGTGA 5 cut(s) 92, 443, 710, 1496, 2794
Hpy166II GTNNAC 8 cut(s) 9, 527, 582, 747, 909, 2229, 2651, 2949
Hpy8I GTNNAC 8 cut(s) 9, 527, 582, 747, 909, 2229, 2651, 2949
HpyCH4III ACNGT 7 cut(s) 151, 327, 1176, 1259, 1716, 1748, 2101
HpyCH4IV ACGT 6 cut(s) 114, 796, 1084, 2034, 2622, 2856
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 1150, 1159, 1229, 1385, 1697
HpyF3I CTNAG 8 cut(s) 71, 193, 497, 540, 1625, 1660, 1930, 2261
HpySE526I ACGT 6 cut(s) 114, 796, 1084, 2034, 2622, 2856
HspAI GCGC 4 cut(s) 82, 2148, 2770, 3007
Ksp22I TGATCA 3 cut(s) 2029, 2377, 2887
KspAI GTTAAC 2 cut(s) 747, 2229
LmnI GCTCC 4 cut(s) 129, 1343, 1925, 2344
Lsp1109I GCAGC 5 cut(s) 91, 1108, 1149, 1177, 1601
LweI GCATC 8 cut(s) 31, 659, 857, 1131, 1162, 1973, 2486, 2781
MaeI CTAG 7 cut(s) 14, 213, 1229, 1479, 1605, 1949, 2087
MaeII ACGT 6 cut(s) 114, 796, 1084, 2034, 2622, 2856
MaeIII GTNAC 5 cut(s) 208, 686, 1600, 1736, 2470
MbiI CCGCTC 1 cut(s) 132
MflI RGATCY 3 cut(s) 1108, 1355, 2839
MhlI GDGCHC 2 cut(s) 142, 2820
MlsI TGGCCA 2 cut(s) 1407, 2247
MluNI TGGCCA 2 cut(s) 1407, 2247
MlyI GAGTC 3 cut(s) 770, 2716, 2902
MmeI TCCRAC 1 cut(s) 2322
Mox20I TGGCCA 2 cut(s) 1407, 2247
Mph1103I ATGCAT 3 cut(s) 780, 1966, 2585
MroXI GAANNNNTTC 3 cut(s) 66, 1297, 1300
MscI TGGCCA 2 cut(s) 1407, 2247
MslI CAYNNNNRTG 5 cut(s) 541, 719, 1467, 2490, 2529
Msp20I TGGCCA 2 cut(s) 1407, 2247
MspCI CTTAAG 1 cut(s) 1045
MspI CCGG 3 cut(s) 103, 912, 1416
MspR9I CCNGG 6 cut(s) 104, 803, 1417, 1527, 1800, 1924
Mva1269I GAATGC 2 cut(s) 508, 2764
MvaI CCWGG 4 cut(s) 803, 1527, 1800, 1924
MvnI CGCG 2 cut(s) 2148, 2150
MwoI GCNNNNNNNGC 6 cut(s) 88, 1150, 1159, 1229, 1385, 1697
NciI CCSGG 2 cut(s) 104, 1417
NcoI CCATGG 1 cut(s) 820
NdeI CATATG 1 cut(s) 1780
NlaIV GGNNCC 5 cut(s) 664, 861, 1420, 1551, 1772
NmuCI GTSAC 2 cut(s) 208, 2470
NsiI ATGCAT 3 cut(s) 780, 1966, 2585
NspI RCATGY 4 cut(s) 95, 1795, 1964, 2636
PaeI GCATGC 1 cut(s) 95
PagI TCATGA 1 cut(s) 2361
PceI AGGCCT 1 cut(s) 2300
PctI GAATGC 2 cut(s) 508, 2764
PdmI GAANNNNTTC 3 cut(s) 66, 1297, 1300
PfeI GAWTC 9 cut(s) 67, 256, 710, 1245, 2290, 2320, 2389, 2534, 2905
PflMI CCANNNNNTGG 2 cut(s) 1781, 2779
PfoI TCCNGGA 2 cut(s) 1798, 1922
PinAI ACCGGT 1 cut(s) 911
PkrI GCNGC 6 cut(s) 81, 134, 1098, 1164, 1167, 1591
PleI GAGTC 3 cut(s) 769, 2715, 2901
PmaCI CACGTG 1 cut(s) 797
PmlI CACGTG 1 cut(s) 797
PpsI GAGTC 3 cut(s) 769, 2715, 2901
Ppu21I YACGTR 2 cut(s) 797, 2035
PpuMI RGGWCCY 2 cut(s) 662, 860
PsiI TTATAA 1 cut(s) 2444
Psp5II RGGWCCY 2 cut(s) 662, 860
Psp6I CCWGG 4 cut(s) 801, 1525, 1798, 1922
PspCI CACGTG 1 cut(s) 797
PspFI CCCAGC 1 cut(s) 2794
PspGI CCWGG 4 cut(s) 801, 1525, 1798, 1922
PspN4I GGNNCC 5 cut(s) 664, 861, 1420, 1551, 1772
PspPI GGNCC 7 cut(s) 662, 785, 860, 906, 1419, 1523, 1549
PspPPI RGGWCCY 2 cut(s) 662, 860
PstI CTGCAG 2 cut(s) 769, 1167
PstNI CAGNNNCTG 3 cut(s) 1162, 1174, 2099
PsuI RGATCY 3 cut(s) 1108, 1355, 2839
RsaI GTAC 6 cut(s) 284, 1261, 1316, 2050, 2184, 2206
RsaNI GTAC 6 cut(s) 283, 1260, 1315, 2049, 2183, 2205
RseI CAYNNNNRTG 5 cut(s) 541, 719, 1467, 2490, 2529
SalI GTCGAC 1 cut(s) 580
SatI GCNGC 6 cut(s) 80, 133, 1097, 1163, 1166, 1590
Sau96I GGNCC 7 cut(s) 662, 785, 860, 906, 1419, 1523, 1549
SchI GAGTC 3 cut(s) 770, 2716, 2902
ScrFI CCNGG 6 cut(s) 104, 803, 1417, 1527, 1800, 1924
SduI GDGCHC 2 cut(s) 142, 2820
SfaNI GCATC 8 cut(s) 31, 659, 857, 1131, 1162, 1973, 2486, 2781
SfcI CTRYAG 4 cut(s) 765, 930, 1068, 1163
SinI GGWCC 5 cut(s) 662, 785, 860, 906, 1549
SmiMI CAYNNNNRTG 5 cut(s) 541, 719, 1467, 2490, 2529
SmlI CTYRAG 5 cut(s) 1045, 1685, 2424, 2873, 2897
SmoI CTYRAG 5 cut(s) 1045, 1685, 2424, 2873, 2897
SpeI ACTAGT 2 cut(s) 212, 1604
SphI GCATGC 1 cut(s) 95
SseBI AGGCCT 1 cut(s) 2300
SsiI CCGC 6 cut(s) 132, 143, 375, 925, 1019, 2654
SspI AATATT 1 cut(s) 304
SspMI CTAG 7 cut(s) 14, 213, 1229, 1479, 1605, 1949, 2087
StuI AGGCCT 1 cut(s) 2300
StyD4I CCNGG 6 cut(s) 102, 801, 1415, 1525, 1798, 1922
StyI CCWWGG 2 cut(s) 820, 2301
TaaI ACNGT 7 cut(s) 151, 327, 1176, 1259, 1716, 1748, 2101
TaiI ACGT 6 cut(s) 117, 799, 1087, 2037, 2625, 2859
TaqI TCGA 7 cut(s) 19, 259, 335, 581, 994, 2604, 2979
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 9 cut(s) 67, 256, 710, 1245, 2290, 2320, 2389, 2534, 2905
TscAI CASTG 2 cut(s) 142, 2104
TseFI GTSAC 2 cut(s) 208, 2470
TseI GCWGC 5 cut(s) 79, 1096, 1162, 1165, 1589
Tsp45I GTSAC 2 cut(s) 208, 2470
TspGWI ACGGA 4 cut(s) 512, 575, 2363, 2677
TspRI CASTG 2 cut(s) 142, 2104
Van91I CCANNNNNTGG 2 cut(s) 1781, 2779
Vha464I CTTAAG 1 cut(s) 1045
VpaK11BI GGWCC 5 cut(s) 662, 785, 860, 906, 1549
XapI RAATTY 8 cut(s) 31, 1197, 1816, 1982, 2012, 2042, 2189, 2433
XceI RCATGY 4 cut(s) 95, 1795, 1964, 2636
XmiI GTMKAC 2 cut(s) 581, 2948
XmnI GAANNNNTTC 3 cut(s) 66, 1297, 1300
XspI CTAG 7 cut(s) 14, 213, 1229, 1479, 1605, 1949, 2087
Zsp2I ATGCAT 3 cut(s) 780, 1966, 2585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.