pycom01g14040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005276
Physical Location & Seq
Reverse (-)
14335230 .. 14336838
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14040.5

Sequence Viewer

Length: 1140 bp
ATGGTTAACAGAGGATTCAAGAAATCCAAAACGAATCAGCTTCCAGGGATTCAAGAAGTCTGGAGGTCTGCTGAGTCAATGACTACAGTTCATAGTGGATGTTCCTCTTTGATGGCAAGCATTGAGTTTGAGCTTTTCGATGACAACAGATCATCTGCGCAGAAACCGACTAGTTTACCCAGTAATCCTGACGCTTCTCCTCGGATGAGGCCATGCCAACCTCCCAAAGGCAAAGTCTTAATGCGCGTGGACCGGAGAAATGAAATGAACCTTGCTGCTGTAAGTAGGAAACTGAACTCATCAGCATCTCGCAAACCGCCAAAGATATCTAGAAGATCAAGTAAAACGCTAACCGGAGCACACACAGTTTTTCATTTTAAAATGCCTTGCTTAGGAGTATCGTGCAGCATCACCAATAGTATTACACCGCCCCCAAAGACATCTCCATTTTGTTCTCCCACTGCCACACACGAGAGCAAAATTGATTCCTCACTTGTCATTTCGGCCTCCCGTGGTTTTACCAAGGGAACTCCTTCGAAATCCACAATGAAAAACAAAGGTGAACGGGAAACTTCTTGTCACCCATCCACTCCAGTGCGCACGCCGCAGTCTTCCCCTCATCCATCACCTGCTAGTTCCCTTGTTGGCTGGTCCTCAGCATCCTCATCAGCGTCTGCGAATCAACGATTGAAGAAGTGTGAGGTCGGTCTGAATACTTTCATCAGCGGACAGGCTTTTTTTGAGAGTGATGTCTCTCGGGAATCAATTCTGGAGAGCCATTCACATGATCAACCATGCATTGGACATGGAAATCAAGAAGCAATGCTCTTGAGTCCACGCGAGGGAAATATGTCAATGGGAGGTAGCCCTATGGATCCTAACAGTGTTTCTAAACATATCAGGCCCTCATGTCTCCGAGCGCCATCTCCAAAGATTGGCTTTTTCGACAAGGAGATTTCTTTGGTGAGATCAAGTGTGCATTCTCCTGCAGGAAGTCACAAGGAAAATAATGCATATTACTTGAAAGAACGAATTGATTGTTTGGCTAAAAATGTTGGTGCCATTGATCTTGGAATTGGATTAATGTTGTGCAGTGAAAGAGGAGATGGAAGGCAAACAAGAGTGCATGTCATTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

41.0

Weight (kDa)

9.74

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 637
Acc16I TGCGCA 2 cut(s) 159, 599
Acc36I ACCTGC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 1058
AccB7I CCANNNNNTGG 2 cut(s) 800, 935
AccII CGCG 2 cut(s) 246, 840
AciI CCGC 4 cut(s) 317, 428, 605, 726
AclWI GGATC 2 cut(s) 869, 882
AfiI CCNNNNNNNGG 5 cut(s) 227, 392, 800, 842, 935
AgsI TTSAA 4 cut(s) 19, 53, 691, 1024
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 593
AluBI AGCT 2 cut(s) 40, 133
AluI AGCT 2 cut(s) 40, 133
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 2 cut(s) 757, 917
AlwI GGATC 2 cut(s) 869, 882
AlwNI CAGNNNCTG 1 cut(s) 674
Ama87I CYCGRG 1 cut(s) 756
AoxI GGCC 3 cut(s) 209, 504, 902
ApeKI GCWGC 2 cut(s) 275, 405
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
AseI ATTAAT 1 cut(s) 1082
Asp700I GAANNNNTTC 3 cut(s) 532, 716, 765
AspLEI GCGC 4 cut(s) 160, 246, 600, 922
AspS9I GGNCC 3 cut(s) 250, 651, 903
AsuHPI GGTGA 5 cut(s) 403, 572, 572, 618, 976
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 2 cut(s) 250, 651
BamHI GGATCC 1 cut(s) 874
BanI GGYRCC 1 cut(s) 1058
BauI CACGAG 1 cut(s) 470
BbsI GAAGAC 1 cut(s) 603
Bbv12I GWGCWC 1 cut(s) 361
BbvCI CCTCAGC 1 cut(s) 655
BbvI GCAGC 2 cut(s) 262, 417
BccI CCATC 5 cut(s) 106, 592, 631, 931, 1100
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 2 cut(s) 757, 917
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 3 cut(s) 171, 330, 633
BfmI CTRYAG 2 cut(s) 84, 987
BfoI RGCGCY 1 cut(s) 923
BfuAI ACCTGC 1 cut(s) 637
BisI GCNGC 3 cut(s) 276, 406, 605
BlsI GCNGC 3 cut(s) 277, 407, 606
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 2 cut(s) 250, 651
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 3 cut(s) 250, 651, 903
BmiI GGNNCC 2 cut(s) 876, 1060
BmrFI CCNGG 1 cut(s) 45
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 314, 417, 668
BmuI ACTGGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 3 cut(s) 82, 576, 791
Bpu10I CCTNAGC 2 cut(s) 391, 655
Bpu14I TTCGAA 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 850
BsaJI CCNNGG 4 cut(s) 44, 200, 511, 522
BsaWI WCCGGW 2 cut(s) 252, 353
Bsc4I CCNNNNNNNGG 5 cut(s) 227, 392, 800, 842, 935
Bse1I ACTGG 2 cut(s) 180, 593
Bse3DI GCAATG 2 cut(s) 828, 1131
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 4 cut(s) 44, 200, 511, 522
BseGI GGATG 5 cut(s) 104, 210, 584, 619, 659
BseLI CCNNNNNNNGG 5 cut(s) 227, 392, 800, 842, 935
BseMI GCAATG 2 cut(s) 828, 1131
BseMII CTCAG 2 cut(s) 63, 669
BseNI ACTGG 2 cut(s) 180, 593
BseRI GAGGAG 2 cut(s) 189, 1116
BseXI GCAGC 2 cut(s) 262, 417
BsgI GTGCAG 2 cut(s) 424, 1111
Bsh1236I CGCG 2 cut(s) 246, 840
BshFI GGCC 3 cut(s) 211, 506, 904
BshNI GGYRCC 1 cut(s) 1058
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 2 cut(s) 253, 354
BslI CCNNNNNNNGG 5 cut(s) 227, 392, 800, 842, 935
BsmAI GTCTC 2 cut(s) 757, 917
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 3 cut(s) 211, 506, 904
BsoBI CYCGRG 1 cut(s) 756
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
BspACI CCGC 4 cut(s) 317, 428, 605, 726
BspANI GGCC 3 cut(s) 211, 506, 904
BspCNI CTCAG 2 cut(s) 64, 668
BspFNI CGCG 2 cut(s) 246, 840
BspLI GGNNCC 2 cut(s) 876, 1060
BspMAI CTGCAG 1 cut(s) 991
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 869, 882
BspT104I TTCGAA 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 1058
BsrDI GCAATG 2 cut(s) 828, 1131
BsrI ACTGG 2 cut(s) 180, 593
BssECI CCNNGG 4 cut(s) 44, 200, 511, 522
BssMI GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 522
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 88, 367, 884
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 2 cut(s) 118, 602
BstDEI CTNAG 3 cut(s) 72, 391, 655
BstDSI CCRYGG 1 cut(s) 511
BstENI CCTNNNNNAGG 2 cut(s) 225, 390
BstF5I GGATG 5 cut(s) 104, 210, 584, 619, 659
BstFNI CGCG 2 cut(s) 246, 840
BstH2I RGCGCY 1 cut(s) 923
BstHHI GCGC 4 cut(s) 160, 246, 600, 922
BstKTI GATC 6 cut(s) 152, 338, 790, 877, 971, 1069
BstMAI GTCTC 2 cut(s) 757, 917
BstMBI GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
BstMWI GCNNNNNNNGC 1 cut(s) 604
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 1130
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 2 cut(s) 84, 987
BstUI CGCG 2 cut(s) 246, 840
BstV1I GCAGC 2 cut(s) 262, 417
BstV2I GAAGAC 1 cut(s) 603
BstX2I RGATCY 1 cut(s) 874
BstYI RGATCY 1 cut(s) 874
BsuRI GGCC 3 cut(s) 211, 506, 904
BtgI CCRYGG 1 cut(s) 511
BtsCI GGATG 5 cut(s) 104, 210, 584, 619, 659
BtsI GCAGTG 2 cut(s) 459, 1099
BtsIMutI CAGTG 4 cut(s) 459, 600, 889, 1099
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 2 cut(s) 118, 602
CaiI CAGNNNCTG 1 cut(s) 674
CfoI GCGC 4 cut(s) 160, 246, 600, 922
Cfr13I GGNCC 3 cut(s) 250, 651, 903
CseI GACGC 2 cut(s) 200, 660
CviAII CATG 6 cut(s) 213, 785, 795, 806, 909, 1127
DdeI CTNAG 3 cut(s) 72, 391, 655
DpnI GATC 6 cut(s) 151, 337, 789, 876, 970, 1068
DpnII GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
DraI TTTAAA 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 522
Eco32I GATATC 1 cut(s) 327
Eco47I GGWCC 2 cut(s) 250, 651
Eco88I CYCGRG 1 cut(s) 756
EcoNI CCTNNNNNAGG 2 cut(s) 225, 390
EcoO109I RGGNCCY 1 cut(s) 903
EcoRII CCWGG 1 cut(s) 43
EcoRV GATATC 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 522
EcoT22I ATGCAT 2 cut(s) 800, 1015
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 6 cut(s) 216, 788, 798, 809, 912, 1130
FalI AAGNNNNNCTT 2 cut(s) 923, 955
FatI CATG 6 cut(s) 212, 784, 794, 805, 908, 1126
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 3 cut(s) 276, 406, 605
FokI GGATG 5 cut(s) 111, 217, 571, 606, 646
Fsp4HI GCNGC 3 cut(s) 276, 406, 605
FspAI RTGCGCAY 1 cut(s) 599
FspBI CTAG 3 cut(s) 171, 330, 633
FspI TGCGCA 2 cut(s) 159, 599
GlaI GCGC 4 cut(s) 159, 245, 599, 921
GluI GCNGC 3 cut(s) 276, 406, 605
GsuI CTGGAG 3 cut(s) 82, 576, 791
HaeII RGCGCY 1 cut(s) 923
HaeIII GGCC 3 cut(s) 211, 506, 904
HapII CCGG 2 cut(s) 253, 354
HgaI GACGC 2 cut(s) 200, 660
HhaI GCGC 4 cut(s) 160, 246, 600, 922
Hin1II CATG 6 cut(s) 216, 788, 798, 809, 912, 1130
Hin6I GCGC 4 cut(s) 158, 244, 598, 920
HinP1I GCGC 4 cut(s) 158, 244, 598, 920
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 8 cut(s) 15, 34, 49, 74, 485, 679, 761, 832
HpaI GTTAAC 1 cut(s) 7
HpaII CCGG 2 cut(s) 253, 354
HphI GGTGA 5 cut(s) 403, 572, 572, 618, 976
Hpy166II GTNNAC 5 cut(s) 7, 176, 250, 563, 836
Hpy188I TCNGA 3 cut(s) 204, 711, 917
Hpy188III TCNNGA 9 cut(s) 19, 53, 61, 188, 330, 758, 770, 815, 829
Hpy8I GTNNAC 5 cut(s) 7, 176, 250, 563, 836
HpyAV CCTTC 2 cut(s) 543, 1104
HpyCH4III ACNGT 3 cut(s) 88, 367, 884
HpyCH4V TGCA 7 cut(s) 405, 798, 979, 989, 1013, 1092, 1126
HpyF10VI GCNNNNNNNGC 1 cut(s) 604
HpyF3I CTNAG 3 cut(s) 72, 391, 655
Hsp92II CATG 6 cut(s) 216, 788, 798, 809, 912, 1130
HspAI GCGC 4 cut(s) 158, 244, 598, 920
Ksp22I TGATCA 1 cut(s) 787
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
LmnI GCTCC 1 cut(s) 356
Lsp1109I GCAGC 2 cut(s) 262, 417
LweI GCATC 3 cut(s) 314, 417, 668
MaeI CTAG 3 cut(s) 171, 330, 633
MaeIII GTNAC 2 cut(s) 578, 995
MalI GATC 6 cut(s) 151, 337, 789, 876, 970, 1068
MboI GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
MboII GAAGA 3 cut(s) 345, 603, 703
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 4 cut(s) 480, 765, 1032, 1074
MlyI GAGTC 2 cut(s) 83, 841
Mph1103I ATGCAT 2 cut(s) 800, 1015
MroXI GAANNNNTTC 3 cut(s) 532, 716, 765
MseI TTAA 4 cut(s) 6, 239, 378, 1082
MslI CAYNNNNRTG 2 cut(s) 593, 783
MspA1I CMGCKG 1 cut(s) 726
MspI CCGG 2 cut(s) 253, 354
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 1 cut(s) 45
MvnI CGCG 2 cut(s) 246, 840
MwoI GCNNNNNNNGC 1 cut(s) 604
NdeII GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
NlaIII CATG 6 cut(s) 216, 788, 798, 809, 912, 1130
NlaIV GGNNCC 2 cut(s) 876, 1060
NmuCI GTSAC 2 cut(s) 578, 995
NsbI TGCGCA 2 cut(s) 159, 599
NsiI ATGCAT 2 cut(s) 800, 1015
NspI RCATGY 1 cut(s) 1130
NspV TTCGAA 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 593
PaqCI CACCTGC 1 cut(s) 637
PctI GAATGC 1 cut(s) 979
PdmI GAANNNNTTC 3 cut(s) 532, 716, 765
PfeI GAWTC 6 cut(s) 15, 34, 49, 485, 679, 761
PflMI CCANNNNNTGG 2 cut(s) 800, 935
PkrI GCNGC 3 cut(s) 277, 407, 606
PleI GAGTC 2 cut(s) 82, 840
PpsI GAGTC 2 cut(s) 82, 840
PshBI ATTAAT 1 cut(s) 1082
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 876, 1060
PspPI GGNCC 3 cut(s) 250, 651, 903
PstI CTGCAG 1 cut(s) 991
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 1 cut(s) 874
RseI CAYNNNNRTG 2 cut(s) 593, 783
SaqAI TTAA 4 cut(s) 6, 239, 378, 1082
SatI GCNGC 3 cut(s) 276, 406, 605
Sau3AI GATC 6 cut(s) 149, 335, 787, 874, 968, 1066
Sau96I GGNCC 3 cut(s) 250, 651, 903
SbfI CCTGCAGG 1 cut(s) 991
SchI GAGTC 2 cut(s) 83, 841
ScrFI CCNGG 1 cut(s) 45
SdaI CCTGCAGG 1 cut(s) 991
SduI GDGCHC 1 cut(s) 361
SetI ASST 9 cut(s) 42, 68, 135, 223, 273, 562, 631, 705, 865
SfaNI GCATC 3 cut(s) 314, 417, 668
SfcI CTRYAG 2 cut(s) 84, 987
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 2 cut(s) 250, 651
SmiMI CAYNNNNRTG 2 cut(s) 593, 783
SmlI CTYRAG 1 cut(s) 829
SmoI CTYRAG 1 cut(s) 829
SpeI ACTAGT 1 cut(s) 170
Sse8387I CCTGCAGG 1 cut(s) 991
Sse9I AATT 4 cut(s) 480, 765, 1032, 1074
SsiI CCGC 4 cut(s) 317, 428, 605, 726
SspMI CTAG 3 cut(s) 171, 330, 633
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 3 cut(s) 88, 367, 884
TaqI TCGA 3 cut(s) 138, 536, 945
TaqII GACCGA 1 cut(s) 695
TasI AATT 4 cut(s) 480, 765, 1032, 1074
TauI GCSGC 1 cut(s) 607
TfiI GAWTC 6 cut(s) 15, 34, 49, 485, 679, 761
Tru1I TTAA 4 cut(s) 6, 239, 378, 1082
Tru9I TTAA 4 cut(s) 6, 239, 378, 1082
TscAI CASTG 4 cut(s) 466, 600, 889, 1099
TseFI GTSAC 2 cut(s) 578, 995
TseI GCWGC 2 cut(s) 275, 405
Tsp45I GTSAC 2 cut(s) 578, 995
TspDTI ATGAA 6 cut(s) 80, 276, 281, 362, 563, 709
TspRI CASTG 4 cut(s) 466, 600, 889, 1099
Van91I CCANNNNNTGG 2 cut(s) 800, 935
VpaK11BI GGWCC 2 cut(s) 250, 651
VspI ATTAAT 1 cut(s) 1082
XagI CCTNNNNNAGG 2 cut(s) 225, 390
XbaI TCTAGA 1 cut(s) 329
XceI RCATGY 1 cut(s) 1130
XmnI GAANNNNTTC 3 cut(s) 532, 716, 765
XspI CTAG 3 cut(s) 171, 330, 633
Zsp2I ATGCAT 2 cut(s) 800, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.