RchiOBHm_Chr1g0364171

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005276
Physical Location & Seq
Reverse (-)
55410893 .. 55418182
7290 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58886

Sequence Viewer

Length: 3921 bp
ATGTTGTCTAGTAGTGCAGATTCAAGTTCCTTCACAATAATGGAAAAATCCTCCACAAACAAAGTAAATGAATGGAGTGAAAAAAGGTCAGCAATTAAGGGAATTCTTGAACCTAAAAATTGGCCAACAAGGAAGGAATTTGTTGATCCCAAGGGGCCATACATTCGAAAGTGGAATATCGTCTTCATAGTATCATGTGTATTAGCTGTTTTACTGGATCCCTTGTTCCTCTACATTCCTACAATTCATGAGGATATGAAGTGTGTGAGATTGGACAAGAACATGAAGATAGCAGCTCTTGTCTTGAGATCAGTCACAGATCTATGCTATCTGTTGAACATCTTTTTCCAACTGTGTGGATCTAAGACCCGTTCAACTATAAGGTCAGGAAGTTGCCCCCCAATGTTAACTAGCTTAAGGATTGTCTTGCTAAGCATAGCTAAGACAATGTGGGAGTCGTACATCATAATTGACATTTTAGCGGTTCTTCCTCTTCCACAGGTAGCGATTCTCATTTTTTTCCCCAAAATGAGGGGATCAGGTTCATCCATTAAAGGGAAGCTTATGAACTTTCTTATTCTAGTGCAATATGTGCCACGAGTTCTTCGTATCTACCTATCGTGCAAGGAATCTAAGAAGTTTTCTAGAGAAGAGACTTCACTATGGCTTAAAGGCATACTCAATTTCTTTATGTACATCCTTGCCAGTCATAGGGAAGCAACTAGGGTTTTGGAAAAGCACAGAAGAGGTTGTTGCCTGTCCGGCGGCGCCCCAGCGTCGATCATAGGTCCAGGCCTCATCGATGAGTGGCGGGTGTTGCCGGACGGGATTGTGGGGTCTTGGGGCTCCAAGTTTTGGTATCCGGTTCAACCTTCTTGGTCGCGTATGACGTTTCTTTCCTCTCAGGCGGCGATGCAGGCGGGCAGGATGGCTGGTTGGCGGTCGGCGCAGGGTTGGAGCAGGTGGTCAGAGGCTGGATCGTGGCGGCGGTTTGCCATGGATCGTACGGCGCTGATTCCTTCTCCGAGTTTGGTCGATCATCGAAGAGTGCAAGGGTGTCGAATTTTCTGGCGGTTGGATGAAGCCGGGCGTCAAGGCACGACGAGTTTCTCACTGATACTCGGTGTGGGCTCATCGAGATCTGGCTTTATGCCTGGATTTGGCCGACCAAAGGTGAGGTTGGTGTGGCTTGCTGCGTGGATGGAGTCTTTCCGGCGGTGCGAGTTTGACGGGACAGATCTATGGTTGTGGTCTGCAGGCAGGTCAACTGAAGTGGAGGAAGATGATTGCGGCGGCTGGGCTATATTTTTGTTTAAAGTAGTTTTCTTGGTTAGGAGTTCGATTAGGGTTTTGGTTAGGTTTTTCTGGTTTTTAGCATGTCCCTACTCTGTTGAGCTAGGGTTGGGTCAAATTGAGGTACCATGTATGTGGTGTTTTTCCTGGGGACGAATTGGTTTATTGTCGGTTTTATCTTATGGTCAATGTGCTGACGAGCGATCCTTGCACGTGGCCTGGCATCTTTGGGACACGGTGTGGAAGAGGGCGTTGGTTGTTGTTGCGGTTGTCTATGAGGTGGTATTGGGAGCCCTTTGTTACTTTTTTGCAGTTCAAAGAATTACAAGTTGCTGGCGACATGCTTGTCGGAATGAAAATGGATGTGACCGTAATAATTTTGACTGCCACAATCACCATACGTTGAGAAATATAACGTCTCTTAATGATTTATGTCCTATAGATCCATCTGATCCAAAAATTTTCGACTTTGGCATATTTCTTCATATCCTCCAGTTTGGTATACTGGGGTCAACTAACTATCCGGAGAAGTTCTCAAATTGCTTTTGGTGGGGCTTGCGACATTTGAGTTCTCTTGGCTCAAACCTCGAACCCAGTATCAATACATGGGAAAACCTCTTTGCGGCTTTTATTTCTATAATTGGGTTGCTGCTATTTTTATATCTCATTGGAAATTTGCAGACTTATATGCAGCTGGATACCGCAAGATTGGAGACGGATAGATGGAATCTGAGAGTTGAAAACAAGTTGGATGAGAAAAGCCGAGAATTCGAACCGTGGTTAGTTAAAAATGGCATCCCTTCGCATAAAAAACCGGACATCATGGCTAAGGTACTAGTAGAGCTTAAACAAAACAGAGATGTGGATATAGAGAATTTACTCTCTATTCTGCCTTCCGATCTTCAAAGCTATGTGGAAAGTTGTATGCCGTTGAGTAGGCTAAAGAAGGTGCCATCAGTTCAAAATATGGCTGAAGCAGTATTGAGAGAAATCTCTGAAAATCTTAAGCCCAAGAAGTACAAAAAGGATAATATCATTATTTCCGAGGATAAGCCACTTGAGATGATGATCTTCATTGTGGATGGACTTGTAAGCATAGAAAAGAGAGATGCGTCTGGTACACTGCAACGAGGTGCAGGACAGTTATTTGGTGAACAACTTCTATGTTGGCCAGCATGGACATCTTTTCCCGGCATATCAAATGAGTCTGCTAGAGCCATTGGTGATGTTGACGTCCTCGTTCTCATGGCGAGTGACATGGAGCGTGTAGGCTATAAATTCAGATCGGAATTTGGTAAAGAAATTACCCCTCTGTCGGATGCTAAGTGGGTTCTTTTAGAGAGCCGCCAGGTGGCTATGTTAAGGAAAGTGCCAAAGTTGGAAACTATGCATACTGAAGTGTTGAAAGCAATTTCTCTGCATCTTAAGCCTAGGAGCTATAGCAAGGCCTGGTTGACTGATACGAATGAACCACTTCGTATGATGATCTTCGTCATAGATGGATCAGTGGAAGAATATGGTACGCTTGATCATGGACGGTTAAGGCACAAAGCAGGATATTTCTCGGGAGAAGAACTTCTGGATTACGTATTAGATAGTTCATTTCCTGCCCTATCACCCTTACCCACCTGTCGGCTCAATGCTAGTAATTATGTTGACATCCTTTATGTCACGGCCAAGGACTTGGCAAGTGTAGCCTCTGAATACAGGTCGAATTTCAGCAGAGTGATCGCCCATCCAACCGATCTTTTTGCTTCCCTCACGTTGCACTGGCTAAAGAAAGTGCCAATGTTCCGAGATATGGATGAAGAAGTGGTGAAAGAAATCTCTAAACGTATGAAGCGTGTGAGCTATAGCAGCGATACTGTTATTATTGAAAAGCACAAACCACTTGAAATGATGTTCGTCTTATTAGGCGGAGAAAGGGTAACTGTTAAAGAGACATATCCTTATCACTGTGTCGTGGGAGATTTCATTGGATTAGAACTTGTACATTGGATTACAAACTGGGCGGCACATACTAATTTTCCTGCTAAATTACCCTTGTCCCACAATTCTGCTAAAGTCCCCAATGATGATCAAATAGGTAATGCCGAAGTCGCTGTTCTCTGGGCCGATGACTTGAAGAGCATAGTATCTGAATTCAGGTCAGACTTCATGAAGCAAACCACCCTCCCAATAGATTCGGAGGGGAAGCTAGCAACTTTTGACCCATTGACTATGGTTAAGAAAGTGCCAATACTGGAAAATATGGATGAAGAAGTATTGAAAGCAATCTGTTCACATCTCAACCCCATGAAATATAGTGATGATACGCCGGACGGGCCCTTTATTTTGAACTCGAATGAAAAGATTGATAGGATGTTCTTCATCATAAGAGGAATTGTATCCTATTGTTCATTCAGCGGACCGGTATATTACCATTATTCAGGAAATTACTTCGGAGAACAACTTCTCGGATGGGTATTAGATGCCTCCGTTCACAGAGTACCCGAGTTGGACCAGTGGGACGCATATATGAGTGTGGGGGATGTTGAAGTCCTTGTTCTCATGGCCGAAGACTTGGCCAAGGTCATCTCTGAATTCGAGACCCATTTCGTCAGGCAGATTCCCCAGGATTCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans Pkinase_Tyr cNMP_binding

Protein Analysis

1306

Amino Acids

148.92

Weight (kDa)

7.68

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 769 - 853 1.6e-06 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 951
AasI GACNNNNNNGTC 1 cut(s) 1638
AatII GACGTC 1 cut(s) 2529
Acc36I ACCTGC 2 cut(s) 951, 1251
Acc65I GGTACC 1 cut(s) 1417
AccB1I GGYRCC 3 cut(s) 767, 1417, 2242
AccB7I CCANNNNNTGG 1 cut(s) 855
AccI GTMKAC 1 cut(s) 1795
AccII CGCG 1 cut(s) 883
AccIII TCCGGA 1 cut(s) 1816
AcoI YGGCCR 6 cut(s) 122, 1162, 2462, 2967, 3846, 3858
AcuI CTGAAG 3 cut(s) 1290, 2286, 2709
AcvI CACGTG 1 cut(s) 1507
AcyI GRCGYC 3 cut(s) 768, 1090, 2526
AdeI CACNNNGTG 1 cut(s) 1533
AflII CTTAAG 3 cut(s) 415, 2297, 2717
AgeI ACCGGT 1 cut(s) 3703
AhlI ACTAGT 1 cut(s) 2128
AjnI CCWGG 7 cut(s) 790, 1153, 1439, 1511, 2640, 2741, 3906
AjuI GAANNNNNNNTTGG 2 cut(s) 1529, 1561
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
Alw26I GTCTC 5 cut(s) 647, 1716, 2000, 3227, 3875
AlwNI CAGNNNCTG 1 cut(s) 974
Ama87I CYCGRG 2 cut(s) 2857, 3785
Aor13HI TCCGGA 1 cut(s) 1816
ApaI GGGCCC 1 cut(s) 3621
ApeKI GCWGC 5 cut(s) 293, 1193, 1942, 1984, 3150
AsiGI ACCGGT 1 cut(s) 3703
Asp700I GAANNNNTTC 4 cut(s) 2451, 2766, 2868, 3744
Asp718I GGTACC 1 cut(s) 1417
AspA2I CCTAGG 1 cut(s) 2723
AspLEI GCGC 3 cut(s) 770, 949, 1012
AspS9I GGNCC 7 cut(s) 155, 788, 3405, 3617, 3618, 3701, 3793
AsuC2I CCSGG 2 cut(s) 1087, 2484
AsuHPI GGTGA 6 cut(s) 1186, 1679, 2456, 2528, 2901, 3121
AsuII TTCGAA 2 cut(s) 166, 2064
AsuNHI GCTAGC 1 cut(s) 3490
AvaI CYCGRG 2 cut(s) 2857, 3785
AvaII GGWCC 3 cut(s) 788, 3701, 3793
AvrII CCTAGG 1 cut(s) 2723
BaeGI GKGCMC 1 cut(s) 3621
BalI TGGCCA 3 cut(s) 124, 2464, 3860
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 3 cut(s) 767, 1417, 2242
BanII GRGCYC 4 cut(s) 848, 1133, 1588, 3621
BauI CACGAG 1 cut(s) 597
BbrPI CACGTG 1 cut(s) 1507
BbsI GAAGAC 2 cut(s) 175, 3858
BbvI GCAGC 5 cut(s) 305, 1180, 1929, 1996, 3162
BccI CCATC 9 cut(s) 922, 1195, 1747, 2010, 2254, 2369, 2786, 3036, 3747
BceAI ACGGC 3 cut(s) 1023, 2206, 2982
BcgI CGANNNNNNTGC 6 cut(s) 1040, 1074, 3004, 3026, 3038, 3060
BciT130I CCWGG 7 cut(s) 792, 1155, 1441, 1513, 2642, 2743, 3908
BciVI GTATCC 3 cut(s) 870, 1984, 3691
BclI TGATCA 2 cut(s) 2821, 3370
BcnI CCSGG 2 cut(s) 1087, 2484
BcoDI GTCTC 5 cut(s) 647, 1716, 2000, 3227, 3875
BcuI ACTAGT 1 cut(s) 2128
BfmI CTRYAG 4 cut(s) 1254, 1731, 2731, 3145
BfoI RGCGCY 2 cut(s) 771, 1013
BfrI CTTAAG 3 cut(s) 415, 2297, 2717
BfuAI ACCTGC 2 cut(s) 951, 1251
BfuI GTATCC 3 cut(s) 870, 1984, 3691
BglI GCCNNNNNGGC 2 cut(s) 762, 3616
BglII AGATCT 3 cut(s) 319, 1139, 1237
BlnI CCTAGG 1 cut(s) 2723
BlpI GCTNAGC 1 cut(s) 431
Bme1390I CCNGG 9 cut(s) 792, 1087, 1155, 1441, 1513, 2484, 2642, 2743, 3908
Bme18I GGWCC 3 cut(s) 788, 3701, 3793
BmeT110I CYCGRG 2 cut(s) 2857, 3785
BmgT120I GGNCC 7 cut(s) 155, 788, 3405, 3617, 3618, 3701, 3793
BmiI GGNNCC 8 cut(s) 156, 219, 769, 847, 1419, 1585, 2244, 3619
BmrFI CCNGG 9 cut(s) 792, 1087, 1155, 1441, 1513, 2484, 2642, 2743, 3908
BmrI ACTGGG 3 cut(s) 1808, 1881, 3310
BmsI GCATC 7 cut(s) 903, 1525, 2097, 2392, 2602, 2722, 3754
BmtI GCTAGC 1 cut(s) 3494
BmuI ACTGGG 3 cut(s) 1808, 1881, 3310
BpiI GAAGAC 2 cut(s) 175, 3858
BplI GAGNNNNNCTC 2 cut(s) 1811, 1843
BpmI CTGGAG 1 cut(s) 1769
Bpu10I CCTNAGC 1 cut(s) 2121
Bpu1102I GCTNAGC 1 cut(s) 431
Bpu14I TTCGAA 2 cut(s) 166, 2064
BpuEI CTTGAG 2 cut(s) 325, 2372
BpuMI CCSGG 2 cut(s) 1087, 2484
Bsa29I ATCGAT 1 cut(s) 801
BsaAI YACGTR 2 cut(s) 1507, 2881
BsaBI GATNNNNATC 1 cut(s) 2360
BsaHI GRCGYC 3 cut(s) 768, 1090, 2526
BsaI GGTCTC 1 cut(s) 3875
BsaJI CCNNGG 9 cut(s) 150, 996, 1440, 2069, 2337, 2723, 2970, 3861, 3906
BsaWI WCCGGW 4 cut(s) 862, 1816, 2107, 3703
BsaXI ACNNNNNCTCC 2 cut(s) 3204, 3234
Bse118I RCCGGY 1 cut(s) 3703
Bse1I ACTGG 9 cut(s) 219, 705, 1786, 1803, 1887, 3068, 3305, 3540, 3796
Bse8I GATNNNNATC 1 cut(s) 2360
BseAI TCCGGA 1 cut(s) 1816
BseBI CCWGG 7 cut(s) 792, 1155, 1441, 1513, 2642, 2743, 3908
BseCI ATCGAT 1 cut(s) 801
BseDI CCNNGG 9 cut(s) 150, 996, 1440, 2069, 2337, 2723, 2970, 3861, 3906
BseJI GATNNNNATC 1 cut(s) 2360
BseMII CTCAG 2 cut(s) 917, 2015
BseNI ACTGG 9 cut(s) 219, 705, 1786, 1803, 1887, 3068, 3305, 3540, 3796
BseSI GKGCMC 1 cut(s) 3621
BseXI GCAGC 5 cut(s) 305, 1180, 1929, 1996, 3162
BseYI CCCAGC 2 cut(s) 772, 1296
BsgI GTGCAG 2 cut(s) 36, 2448
Bsh1236I CGCG 1 cut(s) 883
Bsh1285I CGRYCG 1 cut(s) 944
BshNI GGYRCC 3 cut(s) 767, 1417, 2242
BshTI ACCGGT 1 cut(s) 3703
BshVI ATCGAT 1 cut(s) 801
BsiEI CGRYCG 1 cut(s) 944
BsiHKCI CYCGRG 2 cut(s) 2857, 3785
BsiWI CGTACG 1 cut(s) 1004
BslFI GGGAC 7 cut(s) 1246, 1365, 1458, 1538, 3325, 3344, 3815
BsmAI GTCTC 5 cut(s) 647, 1716, 2000, 3227, 3875
BsmBI CGTCTC 2 cut(s) 1716, 2000
BsmFI GGGAC 7 cut(s) 1246, 1365, 1458, 1538, 3325, 3344, 3815
Bso31I GGTCTC 1 cut(s) 3875
BsoBI CYCGRG 2 cut(s) 2857, 3785
Bsp119I TTCGAA 2 cut(s) 166, 2064
Bsp120I GGGCCC 1 cut(s) 3617
Bsp1286I GDGCHC 4 cut(s) 848, 1133, 1588, 3621
Bsp13I TCCGGA 1 cut(s) 1816
Bsp1407I TGTACA 2 cut(s) 693, 3283
Bsp1720I GCTNAGC 1 cut(s) 431
Bsp19I CCATGG 1 cut(s) 996
BspCNI CTCAG 2 cut(s) 916, 2016
BspDI ATCGAT 1 cut(s) 801
BspEI TCCGGA 1 cut(s) 1816
BspFNI CGCG 1 cut(s) 883
BspHI TCATGA 2 cut(s) 247, 3450
BspLI GGNNCC 8 cut(s) 156, 219, 769, 847, 1419, 1585, 2244, 3619
BspMAI CTGCAG 1 cut(s) 1258
BspMI ACCTGC 2 cut(s) 951, 1251
BspOI GCTAGC 1 cut(s) 3494
BspQI GCTCTTC 1 cut(s) 3413
BspT104I TTCGAA 2 cut(s) 166, 2064
BspT107I GGYRCC 3 cut(s) 767, 1417, 2242
BspTI CTTAAG 3 cut(s) 415, 2297, 2717
BspTNI GGTCTC 1 cut(s) 3875
BsrFI RCCGGY 1 cut(s) 3703
BsrGI TGTACA 2 cut(s) 693, 3283
BsrI ACTGG 9 cut(s) 219, 705, 1786, 1803, 1887, 3068, 3305, 3540, 3796
BssAI RCCGGY 1 cut(s) 3703
BssECI CCNNGG 9 cut(s) 150, 996, 1440, 2069, 2337, 2723, 2970, 3861, 3906
BssNAI GTATAC 1 cut(s) 1796
BssNI GRCGYC 3 cut(s) 768, 1090, 2526
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 5 cut(s) 150, 996, 2723, 2970, 3861
Bst1107I GTATAC 1 cut(s) 1796
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 7 cut(s) 792, 1155, 1441, 1513, 2642, 2743, 3908
Bst4CI ACNGT 9 cut(s) 354, 1531, 1664, 2070, 2436, 2832, 3160, 3226, 3251
Bst6I CTCTTC 6 cut(s) 498, 645, 739, 1039, 1532, 3413
BstACI GRCGYC 3 cut(s) 768, 1090, 2526
BstAFI CTTAAG 3 cut(s) 415, 2297, 2717
BstAPI GCANNNNNTGC 1 cut(s) 592
BstAUI TGTACA 2 cut(s) 693, 3283
BstBAI YACGTR 2 cut(s) 1507, 2881
BstBI TTCGAA 2 cut(s) 166, 2064
BstC8I GCNNGC 8 cut(s) 918, 922, 1191, 1258, 1628, 1850, 2466, 3492
BstDEI CTNAG 8 cut(s) 363, 431, 441, 633, 903, 2024, 2121, 2616
BstDSI CCRYGG 2 cut(s) 996, 2069
BstFNI CGCG 1 cut(s) 883
BstH2I RGCGCY 2 cut(s) 771, 1013
BstHHI GCGC 3 cut(s) 770, 949, 1012
BstMAI GTCTC 5 cut(s) 647, 1716, 2000, 3227, 3875
BstMCI CGRYCG 1 cut(s) 944
BstNI CCWGG 7 cut(s) 792, 1155, 1441, 1513, 2642, 2743, 3908
BstNSI RCATGY 2 cut(s) 1380, 1637
BstSCI CCNGG 9 cut(s) 790, 1085, 1153, 1439, 1511, 2482, 2640, 2741, 3906
BstSFI CTRYAG 4 cut(s) 1254, 1731, 2731, 3145
BstSLI GKGCMC 1 cut(s) 3621
BstSNI TACGTA 1 cut(s) 2881
BstUI CGCG 1 cut(s) 883
BstV1I GCAGC 5 cut(s) 305, 1180, 1929, 1996, 3162
BstV2I GAAGAC 2 cut(s) 175, 3858
BstX2I RGATCY 6 cut(s) 217, 319, 359, 1139, 1237, 1735
BstXI CCANNNNNNTGG 3 cut(s) 356, 1428, 2977
BstYI RGATCY 6 cut(s) 217, 319, 359, 1139, 1237, 1735
BstZ17I GTATAC 1 cut(s) 1796
Bsu15I ATCGAT 1 cut(s) 801
BsuI GTATCC 3 cut(s) 870, 1984, 3691
BsuTUI ATCGAT 1 cut(s) 801
BtgI CCRYGG 2 cut(s) 996, 2069
BtgZI GCGATG 1 cut(s) 926
BtsI GCAGTG 1 cut(s) 2414
BtsIMutI CAGTG 6 cut(s) 1112, 2414, 2805, 3061, 3247, 3803
BveI ACCTGC 2 cut(s) 951, 1251
Cac8I GCNNGC 8 cut(s) 918, 922, 1191, 1258, 1628, 1850, 2466, 3492
CaiI CAGNNNCTG 1 cut(s) 974
CciI TCATGA 2 cut(s) 247, 3450
CfoI GCGC 3 cut(s) 770, 949, 1012
Cfr10I RCCGGY 1 cut(s) 3703
Cfr13I GGNCC 7 cut(s) 155, 788, 3405, 3617, 3618, 3701, 3793
ClaI ATCGAT 1 cut(s) 801
CpoI CGGWCCG 1 cut(s) 3701
CseI GACGC 4 cut(s) 765, 1079, 2394, 3812
CspAI ACCGGT 1 cut(s) 3703
CspCI CAANNNNNGTGG 2 cut(s) 1254, 1289
CspI CGGWCCG 1 cut(s) 3701
DdeI CTNAG 8 cut(s) 363, 431, 441, 633, 903, 2024, 2121, 2616
DinI GGCGCC 1 cut(s) 769
DraI TTTAAA 1 cut(s) 1315
DraIII CACNNNGTG 1 cut(s) 1533
DrdI GACNNNNNNGTC 1 cut(s) 1638
DseDI GACNNNNNNGTC 1 cut(s) 1638
EaeI YGGCCR 6 cut(s) 122, 1162, 2462, 2967, 3846, 3858
Eam1104I CTCTTC 6 cut(s) 498, 645, 739, 1039, 1532, 3413
EarI CTCTTC 6 cut(s) 498, 645, 739, 1039, 1532, 3413
EciI GGCGGA 1 cut(s) 3225
Eco105I TACGTA 1 cut(s) 2881
Eco130I CCWWGG 5 cut(s) 150, 996, 2723, 2970, 3861
Eco147I AGGCCT 2 cut(s) 795, 2741
Eco24I GRGCYC 4 cut(s) 848, 1133, 1588, 3621
Eco31I GGTCTC 1 cut(s) 3875
Eco47I GGWCC 3 cut(s) 788, 3701, 3793
Eco57I CTGAAG 3 cut(s) 1290, 2286, 2709
Eco72I CACGTG 1 cut(s) 1507
Eco88I CYCGRG 2 cut(s) 2857, 3785
EcoO109I RGGNCCY 1 cut(s) 3618
EcoRI GAATTC 4 cut(s) 102, 2060, 3434, 3875
EcoRII CCWGG 7 cut(s) 790, 1153, 1439, 1511, 2640, 2741, 3906
EcoT14I CCWWGG 5 cut(s) 150, 996, 2723, 2970, 3861
EcoT22I ATGCAT 1 cut(s) 2685
EcoT38I GRGCYC 4 cut(s) 848, 1133, 1588, 3621
EgeI GGCGCC 1 cut(s) 769
EheI GGCGCC 1 cut(s) 769
ErhI CCWWGG 5 cut(s) 150, 996, 2723, 2970, 3861
Esp3I CGTCTC 2 cut(s) 1716, 2000
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 7 cut(s) 1246, 1365, 1458, 1538, 3325, 3344, 3815
FauI CCCGC 2 cut(s) 804, 913
FbaI TGATCA 2 cut(s) 2821, 3370
FblI GTMKAC 1 cut(s) 1795
FriOI GRGCYC 4 cut(s) 848, 1133, 1588, 3621
GlaI GCGC 3 cut(s) 769, 948, 1011
GsaI CCCAGC 2 cut(s) 776, 1300
GsuI CTGGAG 1 cut(s) 1769
HaeII RGCGCY 2 cut(s) 771, 1013
HgaI GACGC 4 cut(s) 765, 1079, 2394, 3812
HhaI GCGC 3 cut(s) 770, 949, 1012
Hin1I GRCGYC 3 cut(s) 768, 1090, 2526
Hin6I GCGC 3 cut(s) 768, 947, 1010
HinP1I GCGC 3 cut(s) 768, 947, 1010
HincII GTYRAC 6 cut(s) 408, 1266, 1806, 2524, 2748, 2950
HindII GTYRAC 6 cut(s) 408, 1266, 1806, 2524, 2748, 2950
HindIII AAGCTT 1 cut(s) 560
HpaI GTTAAC 1 cut(s) 408
HphI GGTGA 6 cut(s) 1186, 1679, 2456, 2528, 2901, 3121
Hpy99I CGWCG 2 cut(s) 781, 1105
HpyAV CCTTC 7 cut(s) 40, 127, 882, 1029, 2103, 2196, 2233
HpyCH4III ACNGT 9 cut(s) 354, 1531, 1664, 2070, 2436, 2832, 3160, 3226, 3251
HpyCH4IV ACGT 8 cut(s) 890, 1506, 1694, 1709, 2526, 2880, 3056, 3127
HpyF3I CTNAG 8 cut(s) 363, 431, 441, 633, 903, 2024, 2121, 2616
HpySE526I ACGT 8 cut(s) 890, 1506, 1694, 1709, 2526, 2880, 3056, 3127
Hsp92I GRCGYC 3 cut(s) 768, 1090, 2526
HspAI GCGC 3 cut(s) 768, 947, 1010
KasI GGCGCC 1 cut(s) 767
Kpn2I TCCGGA 1 cut(s) 1816
KpnI GGTACC 1 cut(s) 1421
Ksp22I TGATCA 2 cut(s) 2821, 3370
KspAI GTTAAC 1 cut(s) 408
LguI GCTCTTC 1 cut(s) 3413
LmnI GCTCC 5 cut(s) 851, 957, 1583, 2554, 2727
Lsp1109I GCAGC 5 cut(s) 305, 1180, 1929, 1996, 3162
LweI GCATC 7 cut(s) 903, 1525, 2097, 2392, 2602, 2722, 3754
MaeII ACGT 8 cut(s) 890, 1506, 1694, 1709, 2526, 2880, 3056, 3127
MaeIII GTNAC 6 cut(s) 313, 1592, 1658, 2546, 2962, 3220
MflI RGATCY 6 cut(s) 217, 319, 359, 1139, 1237, 1735
MhlI GDGCHC 4 cut(s) 848, 1133, 1588, 3621
MlsI TGGCCA 3 cut(s) 124, 2464, 3860
MluNI TGGCCA 3 cut(s) 124, 2464, 3860
Mly113I GGCGCC 1 cut(s) 768
MlyI GAGTC 3 cut(s) 464, 1214, 2507
MmeI TCCRAC 9 cut(s) 373, 935, 1056, 1622, 2022, 2589, 2652, 3056, 3771
Mox20I TGGCCA 3 cut(s) 124, 2464, 3860
Mph1103I ATGCAT 1 cut(s) 2685
MroI TCCGGA 1 cut(s) 1816
MroXI GAANNNNTTC 4 cut(s) 2451, 2766, 2868, 3744
MscI TGGCCA 3 cut(s) 124, 2464, 3860
MslI CAYNNNNRTG 2 cut(s) 38, 1426
Msp20I TGGCCA 3 cut(s) 124, 2464, 3860
MspA1I CMGCKG 2 cut(s) 1987, 3699
MspCI CTTAAG 3 cut(s) 415, 2297, 2717
MspR9I CCNGG 9 cut(s) 792, 1087, 1155, 1441, 1513, 2484, 2642, 2743, 3908
MvaI CCWGG 7 cut(s) 792, 1155, 1441, 1513, 2642, 2743, 3908
MvnI CGCG 1 cut(s) 883
NarI GGCGCC 1 cut(s) 768
NciI CCSGG 2 cut(s) 1087, 2484
NcoI CCATGG 1 cut(s) 996
NheI GCTAGC 1 cut(s) 3490
NlaIV GGNNCC 8 cut(s) 156, 219, 769, 847, 1419, 1585, 2244, 3619
NmeAIII GCCGAG 1 cut(s) 2081
NmuCI GTSAC 4 cut(s) 313, 1658, 2546, 2962
NsiI ATGCAT 1 cut(s) 2685
NspI RCATGY 2 cut(s) 1380, 1637
NspV TTCGAA 2 cut(s) 166, 2064
PagI TCATGA 2 cut(s) 247, 3450
PaqCI CACCTGC 1 cut(s) 951
PceI AGGCCT 2 cut(s) 795, 2741
PciSI GCTCTTC 1 cut(s) 3413
PcsI WCGNNNNNNNCGW 3 cut(s) 604, 887, 3133
PdmI GAANNNNTTC 4 cut(s) 2451, 2766, 2868, 3744
PfeI GAWTC 8 cut(s) 20, 508, 629, 1015, 2020, 3476, 3901, 3911
Pfl23II CGTACG 1 cut(s) 1004
PflMI CCANNNNNTGG 1 cut(s) 855
PinAI ACCGGT 1 cut(s) 3703
PleI GAGTC 3 cut(s) 463, 1213, 2506
PluTI GGCGCC 1 cut(s) 771
PmaCI CACGTG 1 cut(s) 1507
PmlI CACGTG 1 cut(s) 1507
PpsI GAGTC 3 cut(s) 463, 1213, 2506
Ppu21I YACGTR 2 cut(s) 1507, 2881
Psp6I CCWGG 7 cut(s) 790, 1153, 1439, 1511, 2640, 2741, 3906
PspCI CACGTG 1 cut(s) 1507
PspFI CCCAGC 2 cut(s) 772, 1296
PspGI CCWGG 7 cut(s) 790, 1153, 1439, 1511, 2640, 2741, 3906
PspLI CGTACG 1 cut(s) 1004
PspN4I GGNNCC 8 cut(s) 156, 219, 769, 847, 1419, 1585, 2244, 3619
PspOMI GGGCCC 1 cut(s) 3617
PspPI GGNCC 7 cut(s) 155, 788, 3405, 3617, 3618, 3701, 3793
PstI CTGCAG 1 cut(s) 1258
PstNI CAGNNNCTG 1 cut(s) 974
PsuI RGATCY 6 cut(s) 217, 319, 359, 1139, 1237, 1735
PvuII CAGCTG 1 cut(s) 1987
RseI CAYNNNNRTG 2 cut(s) 38, 1426
Rsr2I CGGWCCG 1 cut(s) 3701
RsrII CGGWCCG 1 cut(s) 3701
SapI GCTCTTC 1 cut(s) 3413
Sau96I GGNCC 7 cut(s) 155, 788, 3405, 3617, 3618, 3701, 3793
SchI GAGTC 3 cut(s) 464, 1214, 2507
ScrFI CCNGG 9 cut(s) 792, 1087, 1155, 1441, 1513, 2484, 2642, 2743, 3908
SduI GDGCHC 4 cut(s) 848, 1133, 1588, 3621
SfaNI GCATC 7 cut(s) 903, 1525, 2097, 2392, 2602, 2722, 3754
SfcI CTRYAG 4 cut(s) 1254, 1731, 2731, 3145
SfoI GGCGCC 1 cut(s) 769
SfuI TTCGAA 2 cut(s) 166, 2064
SinI GGWCC 3 cut(s) 788, 3701, 3793
SmiMI CAYNNNNRTG 2 cut(s) 38, 1426
SmlI CTYRAG 5 cut(s) 304, 415, 2297, 2351, 2717
SmoI CTYRAG 5 cut(s) 304, 415, 2297, 2351, 2717
SnaBI TACGTA 1 cut(s) 2881
SpeI ACTAGT 1 cut(s) 2128
SseBI AGGCCT 2 cut(s) 795, 2741
SspDI GGCGCC 1 cut(s) 767
StuI AGGCCT 2 cut(s) 795, 2741
StyD4I CCNGG 9 cut(s) 790, 1085, 1153, 1439, 1511, 2482, 2640, 2741, 3906
StyI CCWWGG 5 cut(s) 150, 996, 2723, 2970, 3861
TaaI ACNGT 9 cut(s) 354, 1531, 1664, 2070, 2436, 2832, 3160, 3226, 3251
TaiI ACGT 8 cut(s) 893, 1509, 1697, 1712, 2529, 2883, 3059, 3130
TatI WGTACW 3 cut(s) 693, 2310, 3283
TauI GCSGC 8 cut(s) 768, 911, 988, 1293, 1296, 1919, 2640, 3308
TfiI GAWTC 8 cut(s) 20, 508, 629, 1015, 2020, 3476, 3901, 3911
TscAI CASTG 6 cut(s) 1119, 2421, 2805, 3068, 3254, 3803
TseFI GTSAC 4 cut(s) 313, 1658, 2546, 2962
TseI GCWGC 5 cut(s) 293, 1193, 1942, 1984, 3150
Tsp45I GTSAC 4 cut(s) 313, 1658, 2546, 2962
TspGWI ACGGA 2 cut(s) 2024, 3760
TspRI CASTG 6 cut(s) 1119, 2421, 2805, 3068, 3254, 3803
Van91I CCANNNNNTGG 1 cut(s) 855
Vha464I CTTAAG 3 cut(s) 415, 2297, 2717
VpaK11BI GGWCC 3 cut(s) 788, 3701, 3793
XbaI TCTAGA 1 cut(s) 644
XceI RCATGY 2 cut(s) 1380, 1637
XmaJI CCTAGG 1 cut(s) 2723
XmiI GTMKAC 1 cut(s) 1795
XmnI GAANNNNTTC 4 cut(s) 2451, 2766, 2868, 3744
ZraI GACGTC 1 cut(s) 2527
Zsp2I ATGCAT 1 cut(s) 2685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.