pycom01g15780

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007939
Physical Location & Seq
Forward (+)
15555864 .. 15560731
4868 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1698 bp
ATGATGGGTAGTCCACTTGGTAATGGTAGTTTACCACCCTTATTTGAAAATGCTGCTGCTGCATCTGCAATGGGTGGCTTGGACTCTGGAGCTTTTGGAGGGATGTCTCTAGGACCAAATCTGTTGTCTGCAGCTGCTGATTTGCAGAATATTAGGGTTGGAAATCACGGTGGTGGGAGTGCTCTTCAGGTTCCCATGATGGACCCATTGTATGTTCAGTACTTGAGATCAAATGAATATGCAGCTGCACAGCTTGCAGCCCTTAATGATCCCACAAAAGATAGGGAAGGCATGGGTAATACGTACATGGATTTACTTGGTTTGCAAAAAGCTTATTTGGGGCAATTGCTTTCGCCCCAAAAATCACAATTTGGTGCTCCCTACATTGGCAGGTCTGGTAGCTTGAATCACGGTTATTATGGAAATCCATCATATGTGCATGGGATGTCTTATTCTGGAACACCATTGGGTGGTCCCCTTCTTCCAAACTCTCCTGTTGGACCTGGGAGTCCTGCCAGGCATGGTGAGCGGAACTTGCGTTTTTCTTCTGGGTTGAGGAACATGGGTGGAGGTCTGATGGGAGCTTGGCACTCCGAAAATGGTGGAAATTTGGATGAAAGTTTTGCCTCCTCTTTACTAGATGAGTTCAAAAGCAACAAGACTAAATGTTTTGAACTTTCAGAGATTGCAGGGCATGTTGTTGAGTTCAGTGCCGACCAGTATGGAAGTCGGTTTATTCAACAGAAACTTGAAACTGCCACAATTGAAGAGAAGAACATGGTTTTTGATGAAATTATGCCCCAAGCACTATCTCTTATGACCGATGTGTTTGGTAATTATGTGATTCAGAAGTTTTTTGAGCATGGAACTCCAGCACAAATAAGAGAACTTGCAGACCAGCTCACTGGGCATGTGCTGACCCTTAGCCTTCAAATGTATGGCTGTCGAGTGATTCAGAAGGCTATAGAAGTTGTTGAGCTGGACCAGCAGACTAAAATGGTAGCTGAACTGGATGGTCATGTTATGCGTTGTGTTCGTGATCGAAATGGCAACCATGTTGTCCAGAAGTGTATTGAATGTGTACCTGAAGATGCCATCCAGTTTGTTGTTTCAACTTTTTATGACCAAGTCGTGACACTGTCTACTCATCCATATGGTTGTCGTGTCATACAGAGAGTTTTGGAGCACTGCCATGACCCCAAAACGCAGCAAATAATGATGGATGAGATTTTGCAGTCTGTTTGCATTTTGGCACAAGACCAATATGGAAATTATGTTGTCCAGCATGTGCTGGAACATGGAAAGCCACATGAACGGTCATGCATAATTAAGGAACTAACTGGGCAAATCGTTCAAATGAGCCAGCAGAAGTTTGCGTCAAATGTCATAGAAAAGTGTTTAAGTTTTGGAACTCTTGCTGAACGCCAGGCCCTTGTAACTGAGATGCTTGGTACCACTGACGAAAATGAGCCCCTTCAGGCGATGATGAAGGATCAGTTCGCAAACTATGTTGTACAGAAAGTGTTGGAAACTTGTGACGATCAGCAACTTGAACTAATCCTTAACCGAATTAAGGTTCACCTGAATGCCCTGAAGAAGTATACTTATGGGAAGCATATAGTTGCCCGTGTAGAGAAACTTGTTGCTGCTGGGGAGAGGAGGATTAGCATTCTGGCTCCCCAGGCTTCTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

566

Amino Acids

61.98

Weight (kDa)

5.78

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 1 - 227 7.9e-100 Nucleic acid binding protein NABP
PUF PF00806 232 - 266 1.2e-11 Pumilio-family RNA binding repeat
PUF PF00806 269 - 301 4.2e-09 Pumilio-family RNA binding repeat
PUF PF00806 304 - 337 4.7e-09 Pumilio-family RNA binding repeat
PUF PF00806 376 - 411 2.5e-13 Pumilio-family RNA binding repeat
PUF PF00806 414 - 447 4.3e-10 Pumilio-family RNA binding repeat
PUF PF00806 449 - 482 1.1e-07 Pumilio-family RNA binding repeat
PUF PF00806 495 - 524 4.6e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 507
Acc36I ACCTGC 1 cut(s) 381
Acc65I GGTACC 1 cut(s) 1451
AccB1I GGYRCC 1 cut(s) 1451
AccB7I CCANNNNNTGG 1 cut(s) 470
AccBSI CCGCTC 1 cut(s) 529
AccI GTMKAC 2 cut(s) 1142, 1601
AciI CCGC 1 cut(s) 529
AclWI GGATC 2 cut(s) 263, 1500
AcsI RAATTY 1 cut(s) 607
AcuI CTGAAG 4 cut(s) 170, 1107, 1460, 1613
AfaI GTAC 5 cut(s) 221, 305, 1083, 1453, 1515
AfiI CCNNNNNNNGG 3 cut(s) 386, 470, 1573
AjnI CCWGG 4 cut(s) 502, 515, 1425, 1680
AleI CACNNNNGTG 1 cut(s) 171
Alw21I GWGCWC 3 cut(s) 184, 379, 1188
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 2 cut(s) 263, 1500
AlwNI CAGNNNCTG 1 cut(s) 137
AoxI GGCC 1 cut(s) 1428
ApoI RAATTY 1 cut(s) 607
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp718I GGTACC 1 cut(s) 1451
AspS9I GGNCC 6 cut(s) 113, 202, 473, 500, 982, 1429
AsuHPI GGTGA 2 cut(s) 536, 1571
AvaII GGWCC 5 cut(s) 113, 202, 473, 500, 982
BanI GGYRCC 1 cut(s) 1451
BanII GRGCYC 1 cut(s) 1473
Bbv12I GWGCWC 3 cut(s) 184, 379, 1188
BccI CCATC 6 cut(s) 193, 436, 571, 1007, 1103, 1213
BciT130I CCWGG 4 cut(s) 504, 517, 1427, 1682
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 2 cut(s) 110, 638
BfmI CTRYAG 2 cut(s) 129, 963
BfuAI ACCTGC 1 cut(s) 381
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 4 cut(s) 504, 517, 1427, 1682
Bme18I GGWCC 5 cut(s) 113, 202, 473, 500, 982
BmgT120I GGNCC 6 cut(s) 113, 202, 473, 500, 982, 1429
BmiI GGNNCC 5 cut(s) 192, 204, 475, 1453, 1677
BmrFI CCNGG 4 cut(s) 504, 517, 1427, 1682
BmrI ACTGGG 2 cut(s) 915, 1350
BmsI GCATC 3 cut(s) 71, 1081, 1434
BmuI ACTGGG 2 cut(s) 915, 1350
BpmI CTGGAG 2 cut(s) 108, 855
Bpu10I CCTNAGC 1 cut(s) 923
BpuEI CTTGAG 1 cut(s) 244
BsaAI YACGTR 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 503, 1680
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 470, 1573
Bse1I ACTGG 5 cut(s) 718, 910, 1014, 1099, 1345
Bse3DI GCAATG 1 cut(s) 75
BseBI CCWGG 4 cut(s) 504, 517, 1427, 1682
BseDI CCNNGG 2 cut(s) 503, 1680
BseGI GGATG 7 cut(s) 108, 450, 619, 1018, 1095, 1147, 1228
BseLI CCNNNNNNNGG 3 cut(s) 386, 470, 1573
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 1 cut(s) 1431
BseNI ACTGG 5 cut(s) 718, 910, 1014, 1099, 1345
BseRI GAGGAG 2 cut(s) 619, 1672
BseYI CCCAGC 1 cut(s) 1649
BsgI GTGCAG 1 cut(s) 231
BshFI GGCC 1 cut(s) 1430
BshNI GGYRCC 1 cut(s) 1451
BsiHKAI GWGCWC 3 cut(s) 184, 379, 1188
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 3 cut(s) 386, 470, 1573
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 459
BsmI GAATGC 2 cut(s) 1591, 1668
BsnI GGCC 1 cut(s) 1430
Bsp1286I GDGCHC 4 cut(s) 184, 379, 1188, 1473
Bsp1407I TGTACA 1 cut(s) 1513
Bsp143I GATC 5 cut(s) 227, 268, 1039, 1492, 1540
BspACI CCGC 1 cut(s) 529
BspANI GGCC 1 cut(s) 1430
BspCNI CTCAG 1 cut(s) 1432
BspLI GGNNCC 5 cut(s) 192, 204, 475, 1453, 1677
BspMAI CTGCAG 1 cut(s) 133
BspMI ACCTGC 1 cut(s) 381
BspPI GGATC 2 cut(s) 263, 1500
BspQI GCTCTTC 1 cut(s) 189
BspT107I GGYRCC 1 cut(s) 1451
BsrBI CCGCTC 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 75
BsrGI TGTACA 1 cut(s) 1513
BsrI ACTGG 5 cut(s) 718, 910, 1014, 1099, 1345
BssECI CCNNGG 2 cut(s) 503, 1680
BssMI GATC 5 cut(s) 227, 268, 1039, 1492, 1540
BssNAI GTATAC 1 cut(s) 1602
Bst1107I GTATAC 1 cut(s) 1602
Bst2UI CCWGG 4 cut(s) 504, 517, 1427, 1682
Bst4CI ACNGT 4 cut(s) 170, 413, 1140, 1317
Bst6I CTCTTC 2 cut(s) 189, 762
BstAPI GCANNNNNTGC 1 cut(s) 254
BstAUI TGTACA 1 cut(s) 1513
BstBAI YACGTR 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 255, 1364
BstDEI CTNAG 3 cut(s) 923, 1440, 1695
BstF5I GGATG 7 cut(s) 108, 450, 619, 1018, 1095, 1147, 1228
BstKTI GATC 5 cut(s) 230, 271, 1042, 1495, 1543
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 5 cut(s) 227, 268, 1039, 1492, 1540
BstMWI GCNNNNNNNGC 9 cut(s) 59, 65, 254, 526, 535, 907, 985, 1682, 1691
BstNI CCWGG 4 cut(s) 504, 517, 1427, 1682
BstNSI RCATGY 3 cut(s) 698, 914, 1289
BstSCI CCNGG 4 cut(s) 502, 515, 1425, 1680
BstSFI CTRYAG 2 cut(s) 129, 963
BstSNI TACGTA 1 cut(s) 303
BstXI CCANNNNNNTGG 1 cut(s) 905
BstZ17I GTATAC 1 cut(s) 1602
BsuRI GGCC 1 cut(s) 1430
BtgZI GCGATG 1 cut(s) 1496
BtsCI GGATG 7 cut(s) 108, 450, 619, 1018, 1095, 1147, 1228
BtsI GCAGTG 1 cut(s) 1186
BtsIMutI CAGTG 5 cut(s) 715, 903, 1136, 1186, 1455
BveI ACCTGC 1 cut(s) 381
Cac8I GCNNGC 2 cut(s) 255, 1364
CaiI CAGNNNCTG 1 cut(s) 137
Cfr13I GGNCC 6 cut(s) 113, 202, 473, 500, 982, 1429
CseI GACGC 1 cut(s) 1365
Csp6I GTAC 5 cut(s) 220, 304, 1082, 1452, 1514
CviQI GTAC 5 cut(s) 220, 304, 1082, 1452, 1514
DdeI CTNAG 3 cut(s) 923, 1440, 1695
DpnI GATC 5 cut(s) 229, 270, 1041, 1494, 1542
DpnII GATC 5 cut(s) 227, 268, 1039, 1492, 1540
DrdI GACNNNNNNGTC 1 cut(s) 507
DseDI GACNNNNNNGTC 1 cut(s) 507
Eam1104I CTCTTC 2 cut(s) 189, 762
EarI CTCTTC 2 cut(s) 189, 762
Eco105I TACGTA 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 1473
Eco47I GGWCC 5 cut(s) 113, 202, 473, 500, 982
Eco57I CTGAAG 4 cut(s) 170, 1107, 1460, 1613
EcoO109I RGGNCCY 1 cut(s) 1429
EcoRII CCWGG 4 cut(s) 502, 515, 1425, 1680
EcoT22I ATGCAT 1 cut(s) 1325
EcoT38I GRGCYC 1 cut(s) 1473
FaqI GGGAC 1 cut(s) 459
FauNDI CATATG 2 cut(s) 433, 1153
FblI GTMKAC 2 cut(s) 1142, 1601
FokI GGATG 7 cut(s) 115, 457, 626, 1025, 1082, 1134, 1235
FriOI GRGCYC 1 cut(s) 1473
FspBI CTAG 2 cut(s) 110, 638
GsaI CCCAGC 1 cut(s) 1653
GsuI CTGGAG 2 cut(s) 108, 855
HaeIII GGCC 1 cut(s) 1430
HgaI GACGC 1 cut(s) 1365
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 5 cut(s) 83, 406, 508, 844, 952
HphI GGTGA 2 cut(s) 536, 1571
Hpy166II GTNNAC 6 cut(s) 14, 32, 1082, 1143, 1579, 1602
Hpy188I TCNGA 5 cut(s) 576, 595, 682, 849, 957
Hpy188III TCNNGA 5 cut(s) 87, 456, 1037, 1063, 1132
Hpy8I GTNNAC 6 cut(s) 14, 32, 1082, 1143, 1579, 1602
HpyAV CCTTC 6 cut(s) 281, 488, 938, 952, 1483, 1484
HpyCH4III ACNGT 4 cut(s) 170, 413, 1140, 1317
HpyCH4IV ACGT 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 9 cut(s) 59, 65, 254, 526, 535, 907, 985, 1682, 1691
HpyF3I CTNAG 3 cut(s) 923, 1440, 1695
HpySE526I ACGT 1 cut(s) 302
KpnI GGTACC 1 cut(s) 1455
Kzo9I GATC 5 cut(s) 227, 268, 1039, 1492, 1540
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 5 cut(s) 89, 382, 581, 1183, 1681
LweI GCATC 3 cut(s) 71, 1081, 1434
MaeI CTAG 2 cut(s) 110, 638
MaeII ACGT 1 cut(s) 302
MaeIII GTNAC 3 cut(s) 1132, 1435, 1535
MalI GATC 5 cut(s) 229, 270, 1041, 1494, 1542
MbiI CCGCTC 1 cut(s) 529
MboI GATC 5 cut(s) 227, 268, 1039, 1492, 1540
MboII GAAGA 7 cut(s) 176, 473, 537, 779, 784, 1100, 1606
MfeI CAATTG 2 cut(s) 344, 762
MhlI GDGCHC 4 cut(s) 184, 379, 1188, 1473
MluCI AATT 9 cut(s) 344, 368, 607, 762, 792, 835, 1270, 1326, 1569
MlyI GAGTC 2 cut(s) 77, 517
MmeI TCCRAC 3 cut(s) 139, 478, 1506
MnlI CCTC 7 cut(s) 92, 549, 563, 637, 640, 1650, 1653
Mph1103I ATGCAT 1 cut(s) 1325
MseI TTAA 5 cut(s) 264, 1329, 1400, 1563, 1572
MslI CAYNNNNRTG 4 cut(s) 171, 1152, 1191, 1584
MspA1I CMGCKG 2 cut(s) 134, 245
MspR9I CCNGG 4 cut(s) 504, 517, 1427, 1682
MunI CAATTG 2 cut(s) 344, 762
Mva1269I GAATGC 2 cut(s) 1591, 1668
MvaI CCWGG 4 cut(s) 504, 517, 1427, 1682
MwoI GCNNNNNNNGC 9 cut(s) 59, 65, 254, 526, 535, 907, 985, 1682, 1691
NdeI CATATG 2 cut(s) 433, 1153
NdeII GATC 5 cut(s) 227, 268, 1039, 1492, 1540
NlaIV GGNNCC 5 cut(s) 192, 204, 475, 1453, 1677
NmuCI GTSAC 2 cut(s) 1132, 1535
NsiI ATGCAT 1 cut(s) 1325
NspI RCATGY 3 cut(s) 698, 914, 1289
OliI CACNNNNGTG 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 189
PctI GAATGC 2 cut(s) 1591, 1668
PfeI GAWTC 3 cut(s) 406, 844, 952
PflFI GACNNNGTC 2 cut(s) 1127, 1138
PflMI CCANNNNNTGG 1 cut(s) 470
PleI GAGTC 2 cut(s) 77, 516
PpsI GAGTC 2 cut(s) 77, 516
Ppu21I YACGTR 1 cut(s) 303
Psp6I CCWGG 4 cut(s) 502, 515, 1425, 1680
PspFI CCCAGC 1 cut(s) 1649
PspGI CCWGG 4 cut(s) 502, 515, 1425, 1680
PspN4I GGNNCC 5 cut(s) 192, 204, 475, 1453, 1677
PspPI GGNCC 6 cut(s) 113, 202, 473, 500, 982, 1429
PstI CTGCAG 1 cut(s) 133
PstNI CAGNNNCTG 1 cut(s) 137
PsyI GACNNNGTC 2 cut(s) 1127, 1138
PvuII CAGCTG 2 cut(s) 134, 245
RsaI GTAC 5 cut(s) 221, 305, 1083, 1453, 1515
RsaNI GTAC 5 cut(s) 220, 304, 1082, 1452, 1514
RseI CAYNNNNRTG 4 cut(s) 171, 1152, 1191, 1584
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 5 cut(s) 264, 1329, 1400, 1563, 1572
Sau3AI GATC 5 cut(s) 227, 268, 1039, 1492, 1540
Sau96I GGNCC 6 cut(s) 113, 202, 473, 500, 982, 1429
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 2 cut(s) 77, 517
ScrFI CCNGG 4 cut(s) 504, 517, 1427, 1682
SduI GDGCHC 4 cut(s) 184, 379, 1188, 1473
SfaNI GCATC 3 cut(s) 71, 1081, 1434
SfcI CTRYAG 2 cut(s) 129, 963
SinI GGWCC 5 cut(s) 113, 202, 473, 500, 982
SmiMI CAYNNNNRTG 4 cut(s) 171, 1152, 1191, 1584
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
SnaBI TACGTA 1 cut(s) 303
Sse9I AATT 9 cut(s) 344, 368, 607, 762, 792, 835, 1270, 1326, 1569
SsiI CCGC 1 cut(s) 529
SspI AATATT 1 cut(s) 151
SspMI CTAG 2 cut(s) 110, 638
StyD4I CCNGG 4 cut(s) 502, 515, 1425, 1680
TaaI ACNGT 4 cut(s) 170, 413, 1140, 1317
TaiI ACGT 1 cut(s) 305
TaqI TCGA 2 cut(s) 946, 1042
TaqII GACCGA 1 cut(s) 836
TasI AATT 9 cut(s) 344, 368, 607, 762, 792, 835, 1270, 1326, 1569
TatI WGTACW 2 cut(s) 219, 1513
TfiI GAWTC 3 cut(s) 406, 844, 952
Tru1I TTAA 5 cut(s) 264, 1329, 1400, 1563, 1572
Tru9I TTAA 5 cut(s) 264, 1329, 1400, 1563, 1572
TscAI CASTG 5 cut(s) 715, 910, 1143, 1193, 1462
TseFI GTSAC 2 cut(s) 1132, 1535
Tsp45I GTSAC 2 cut(s) 1132, 1535
TspDTI ATGAA 5 cut(s) 249, 630, 804, 1326, 1502
TspRI CASTG 5 cut(s) 715, 910, 1143, 1193, 1462
Tth111I GACNNNGTC 2 cut(s) 1127, 1138
Van91I CCANNNNNTGG 1 cut(s) 470
VpaK11BI GGWCC 5 cut(s) 113, 202, 473, 500, 982
XapI RAATTY 1 cut(s) 607
XceI RCATGY 3 cut(s) 698, 914, 1289
XmiI GTMKAC 2 cut(s) 1142, 1601
XspI CTAG 2 cut(s) 110, 638
ZrmI AGTACT 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 1325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.