pycom01g18150
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010870
Physical Location & Seq
Forward (+)
17250877 .. 17253415
2539 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1086 bp
ATGTTCGAATCGAAACCAACCCAAAAAAAGCAAAAGCTGCCAGAACCAGAATCCATGGAAGAAATCATTTCTTTAATCCTCCATGGATGCATGTTGGCCGGAAAGCTTAAAGAAACCTTACCCAACTTGGCCACCCAACCTGACTTACTCTCCAATTCTTTGGAAGAAATCACAAAGATTTTTGGTTCAGCAAAAGAAAGGGTTTTACATAGTCAAGGCTCAAGTAGTACTTCATATCTTCACAACCTATTAACACTTGAGCACCAGCAGATTGGAACGAGTGTTGAACAATGGCTGAGGTCTAATACGCAGGCAATGGATATAATCCAAACCCAACTAGCAGCTGATCGAAACGAAGCAAAGGTCAGTGGCGGCGTTGATGTCCGGGTTTCGGAGGCGGCAATTGGGGGTTTTCAGGCAGCTGATGTGTCAGCTTCTAACGTGAACATTGCGTCATCAAGCACACAAAGACCGCGACGAAGGAAAGATCAAGGGGTGATTAACAAAATAACAGTGCCAGCACCTAGGATTGGAAACACTGAAATCCCGCCAGAAGATGGCTTCACCTGGAGAAAATACGGCCAAAAGGAGATCATGAACAGCAGATTCCCAAGATGCACCCATCAAAAGCTGTACAATTGCCCAGCCAAGAAGCAAGTCCAGAGGCTGGACAATGACCCCTTAACATTTGAGGTAATGTACAGAGGTGAACACACATGCCACATGTCAGCCACCGCACCTTCAACTGTACCACCACCAACAGCAGACCATCATATTCCACAAGATCATATGTCAGCAACCACTGAGCCTCCAACATCTCTATGGCTTTCCATGGACTTTAACCCTATTAGACAAGGAGGCAGCAGCAGTGGTATGATCGGTGGCGGTCGTGGCAGTGGTCGTGGCAGCGGTGATGGCGTTGGTACATCCACCGGCACACGTTATGGTAAAGAAGTTGACTTTCCTGTGATGGATTTGGCCGATACTATGTTTAATTCAGGCAGCAGCAGCAGCAGTATGGATTTTATTTTCAATTCTGCTGAACATAGATGGCAGTCAGATGACAAGAAAAATTTAGATCACTAG

Protein Analysis

362

Amino Acids

39.61

Weight (kDa)

6.46

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 185 - 241 1e-19 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 557, 667
AccII CGCG 1 cut(s) 475
AciI CCGC 7 cut(s) 372, 398, 473, 548, 735, 885, 909
AcoI YGGCCR 4 cut(s) 96, 129, 580, 978
AcsI RAATTY 1 cut(s) 1072
AfaI GTAC 5 cut(s) 229, 635, 701, 750, 925
AfiI CCNNNNNNNGG 4 cut(s) 391, 530, 557, 667
AflIII ACRYGT 2 cut(s) 723, 938
AgsI TTSAA 3 cut(s) 287, 744, 1033
AjnI CCWGG 1 cut(s) 566
AluBI AGCT 6 cut(s) 37, 106, 344, 422, 434, 631
AluI AGCT 6 cut(s) 37, 106, 344, 422, 434, 631
Alw21I GWGCWC 1 cut(s) 264
AlwNI CAGNNNCTG 1 cut(s) 667
AoxI GGCC 4 cut(s) 96, 129, 580, 978
ApoI RAATTY 1 cut(s) 1072
ArsI GACNNNNNNTTYG 2 cut(s) 348, 380
AspA2I CCTAGG 1 cut(s) 524
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 4 cut(s) 508, 556, 719, 923
AsuII TTCGAA 1 cut(s) 6
AvrII CCTAGG 1 cut(s) 524
BalI TGGCCA 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 296
BccI CCATC 6 cut(s) 551, 630, 777, 908, 964, 1044
BceAI ACGGC 1 cut(s) 595
BciT130I CCWGG 1 cut(s) 568
BcnI CCSGG 1 cut(s) 386
BfaI CTAG 3 cut(s) 338, 525, 1084
BlnI CCTAGG 1 cut(s) 524
BmcAI AGTACT 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 386, 568
BmrFI CCNGG 2 cut(s) 386, 568
BmsI GCATC 2 cut(s) 77, 605
BpmI CTGGAG 1 cut(s) 589
Bpu10I CCTNAGC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 6
BpuEI CTTGAG 2 cut(s) 205, 278
BpuMI CCSGG 1 cut(s) 386
BsaJI CCNNGG 4 cut(s) 54, 82, 524, 831
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 793, 823
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 530, 557, 667
Bse118I RCCGGY 1 cut(s) 932
Bse3DI GCAATG 2 cut(s) 321, 447
BseBI CCWGG 1 cut(s) 568
BseDI CCNNGG 4 cut(s) 54, 82, 524, 831
BseGI GGATG 2 cut(s) 92, 926
BseLI CCNNNNNNNGG 4 cut(s) 391, 530, 557, 667
BseMI GCAATG 2 cut(s) 321, 447
BseMII CTCAG 2 cut(s) 287, 795
BseYI CCCAGC 1 cut(s) 643
Bsh1236I CGCG 1 cut(s) 475
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 4 cut(s) 98, 131, 582, 980
BsiEI CGRYCG 1 cut(s) 889
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 3 cut(s) 99, 385, 933
BslI CCNNNNNNNGG 4 cut(s) 391, 530, 557, 667
BsnI GGCC 4 cut(s) 98, 131, 582, 980
Bsp119I TTCGAA 1 cut(s) 6
Bsp1286I GDGCHC 1 cut(s) 264
Bsp1407I TGTACA 2 cut(s) 633, 699
Bsp143I GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
Bsp19I CCATGG 3 cut(s) 54, 82, 831
BspACI CCGC 7 cut(s) 372, 398, 473, 548, 735, 885, 909
BspANI GGCC 4 cut(s) 98, 131, 582, 980
BspCNI CTCAG 2 cut(s) 288, 796
BspFNI CGCG 1 cut(s) 475
BspHI TCATGA 1 cut(s) 594
BspT104I TTCGAA 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 321, 447
BsrFI RCCGGY 1 cut(s) 932
BsrGI TGTACA 2 cut(s) 633, 699
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 4 cut(s) 54, 82, 524, 831
BssMI GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
BssT1I CCWWGG 4 cut(s) 54, 82, 524, 831
Bst2UI CCWGG 1 cut(s) 568
Bst4CI ACNGT 2 cut(s) 514, 748
BstAPI GCANNNNNTGC 1 cut(s) 37
BstAUI TGTACA 2 cut(s) 633, 699
BstBI TTCGAA 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 312, 519
BstDEI CTNAG 2 cut(s) 296, 804
BstDSI CCRYGG 3 cut(s) 54, 82, 831
BstF5I GGATG 2 cut(s) 92, 926
BstFNI CGCG 1 cut(s) 475
BstKTI GATC 6 cut(s) 349, 490, 594, 787, 879, 1081
BstMBI GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 5 cut(s) 37, 891, 915, 1008, 1011
BstNI CCWGG 1 cut(s) 568
BstNSI RCATGY 3 cut(s) 94, 720, 727
BstSCI CCNGG 2 cut(s) 384, 566
BstUI CGCG 1 cut(s) 475
BstXI CCANNNNNNTGG 2 cut(s) 160, 272
BsuRI GGCC 4 cut(s) 98, 131, 582, 980
BtgI CCRYGG 3 cut(s) 54, 82, 831
BtsCI GGATG 2 cut(s) 92, 926
BtsI GCAGTG 2 cut(s) 874, 901
BtsIMutI CAGTG 6 cut(s) 373, 519, 537, 801, 874, 901
Cac8I GCNNGC 2 cut(s) 312, 519
CaiI CAGNNNCTG 1 cut(s) 667
CciI TCATGA 1 cut(s) 594
Cfr10I RCCGGY 1 cut(s) 932
CseI GACGC 1 cut(s) 441
Csp6I GTAC 5 cut(s) 228, 634, 700, 749, 924
CviAII CATG 7 cut(s) 55, 83, 91, 595, 717, 724, 832
CviQI GTAC 5 cut(s) 228, 634, 700, 749, 924
DdeI CTNAG 2 cut(s) 296, 804
DpnI GATC 6 cut(s) 348, 489, 593, 786, 878, 1080
DpnII GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
EaeI YGGCCR 4 cut(s) 96, 129, 580, 978
Eco130I CCWWGG 4 cut(s) 54, 82, 524, 831
EcoRII CCWGG 1 cut(s) 566
EcoT14I CCWWGG 4 cut(s) 54, 82, 524, 831
EcoT22I ATGCAT 1 cut(s) 92
ErhI CCWWGG 4 cut(s) 54, 82, 524, 831
FaeI CATG 7 cut(s) 58, 86, 94, 598, 720, 727, 835
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FatI CATG 7 cut(s) 54, 82, 90, 594, 716, 723, 831
FauI CCCGC 1 cut(s) 555
FauNDI CATATG 1 cut(s) 789
FokI GGATG 2 cut(s) 99, 913
FspBI CTAG 3 cut(s) 338, 525, 1084
GsaI CCCAGC 1 cut(s) 647
GsuI CTGGAG 1 cut(s) 589
HaeIII GGCC 4 cut(s) 98, 131, 582, 980
HapII CCGG 3 cut(s) 99, 385, 933
HgaI GACGC 1 cut(s) 441
Hin1II CATG 7 cut(s) 58, 86, 94, 598, 720, 727, 835
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 3 cut(s) 8, 50, 606
HpaII CCGG 3 cut(s) 99, 385, 933
HphI GGTGA 4 cut(s) 508, 556, 719, 923
Hpy166II GTNNAC 3 cut(s) 445, 710, 958
Hpy188I TCNGA 2 cut(s) 394, 1060
Hpy188III TCNNGA 2 cut(s) 595, 661
Hpy8I GTNNAC 3 cut(s) 445, 710, 958
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 2 cut(s) 474, 750
HpyCH4III ACNGT 2 cut(s) 514, 748
HpyCH4IV ACGT 2 cut(s) 441, 940
HpyCH4V TGCA 2 cut(s) 90, 618
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 891, 915, 1008, 1011
HpyF3I CTNAG 2 cut(s) 296, 804
HpySE526I ACGT 2 cut(s) 441, 940
Hsp92II CATG 7 cut(s) 58, 86, 94, 598, 720, 727, 835
Kzo9I GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
LweI GCATC 2 cut(s) 77, 605
MaeI CTAG 3 cut(s) 338, 525, 1084
MaeII ACGT 2 cut(s) 441, 940
MalI GATC 6 cut(s) 348, 489, 593, 786, 878, 1080
MboI GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
MboII GAAGA 4 cut(s) 71, 176, 230, 566
MfeI CAATTG 2 cut(s) 402, 637
MhlI GDGCHC 1 cut(s) 264
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 6 cut(s) 154, 402, 637, 994, 1033, 1072
MluNI TGGCCA 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 836
MnlI CCTC 8 cut(s) 89, 291, 388, 657, 685, 698, 819, 851
Mox20I TGGCCA 1 cut(s) 131
Mph1103I ATGCAT 1 cut(s) 92
MscI TGGCCA 1 cut(s) 131
MseI TTAA 7 cut(s) 74, 108, 251, 501, 683, 840, 993
MslI CAYNNNNRTG 1 cut(s) 820
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 3 cut(s) 344, 422, 909
MspI CCGG 3 cut(s) 99, 385, 933
MspR9I CCNGG 2 cut(s) 386, 568
MunI CAATTG 2 cut(s) 402, 637
MvaI CCWGG 1 cut(s) 568
MvnI CGCG 1 cut(s) 475
MwoI GCNNNNNNNGC 5 cut(s) 37, 891, 915, 1008, 1011
NciI CCSGG 1 cut(s) 386
NcoI CCATGG 3 cut(s) 54, 82, 831
NdeI CATATG 1 cut(s) 789
NdeII GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
NlaIII CATG 7 cut(s) 58, 86, 94, 598, 720, 727, 835
NsiI ATGCAT 1 cut(s) 92
NspI RCATGY 3 cut(s) 94, 720, 727
NspV TTCGAA 1 cut(s) 6
PagI TCATGA 1 cut(s) 594
PciI ACATGT 1 cut(s) 723
PfeI GAWTC 3 cut(s) 8, 50, 606
PflMI CCANNNNNTGG 2 cut(s) 557, 667
PscI ACATGT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 566
PspFI CCCAGC 1 cut(s) 643
PspGI CCWGG 1 cut(s) 566
PstNI CAGNNNCTG 1 cut(s) 667
PvuII CAGCTG 2 cut(s) 344, 422
RsaI GTAC 5 cut(s) 229, 635, 701, 750, 925
RsaNI GTAC 5 cut(s) 228, 634, 700, 749, 924
RseI CAYNNNNRTG 1 cut(s) 820
SaqAI TTAA 7 cut(s) 74, 108, 251, 501, 683, 840, 993
Sau3AI GATC 6 cut(s) 346, 487, 591, 784, 876, 1078
ScaI AGTACT 1 cut(s) 229
ScrFI CCNGG 2 cut(s) 386, 568
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 2 cut(s) 77, 605
SfuI TTCGAA 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 820
SmlI CTYRAG 2 cut(s) 220, 257
SmoI CTYRAG 2 cut(s) 220, 257
Sse9I AATT 6 cut(s) 154, 402, 637, 994, 1033, 1072
SsiI CCGC 7 cut(s) 372, 398, 473, 548, 735, 885, 909
SspMI CTAG 3 cut(s) 338, 525, 1084
StyD4I CCNGG 2 cut(s) 384, 566
StyI CCWWGG 4 cut(s) 54, 82, 524, 831
TaaI ACNGT 2 cut(s) 514, 748
TaiI ACGT 2 cut(s) 444, 943
TaqI TCGA 3 cut(s) 6, 11, 349
TasI AATT 6 cut(s) 154, 402, 637, 994, 1033, 1072
TatI WGTACW 3 cut(s) 227, 633, 699
TauI GCSGC 2 cut(s) 375, 401
TfiI GAWTC 3 cut(s) 8, 50, 606
Tru1I TTAA 7 cut(s) 74, 108, 251, 501, 683, 840, 993
Tru9I TTAA 7 cut(s) 74, 108, 251, 501, 683, 840, 993
TscAI CASTG 6 cut(s) 373, 519, 544, 808, 874, 901
TspDTI ATGAA 2 cut(s) 222, 611
TspRI CASTG 6 cut(s) 373, 519, 544, 808, 874, 901
Van91I CCANNNNNTGG 2 cut(s) 557, 667
XapI RAATTY 1 cut(s) 1072
XceI RCATGY 3 cut(s) 94, 720, 727
XcmI CCANNNNNNNNNTGG 1 cut(s) 819
XmaJI CCTAGG 1 cut(s) 524
XspI CTAG 3 cut(s) 338, 525, 1084
ZrmI AGTACT 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.