Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0003903

Physical Location & Seq

Genomic Coordinates Reverse (-)

17273839 .. 17283132

Gene Structure 9294 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5091 bp

ATGGGTAGAAGGAAGCAAAGGCGGCCGCACCGATCCGGTGGGGTTGTATTGGAGAGTCAAAGCAATGCCGATCAGGCAGAATTGGATGGAATCAAGATTTCAAATGGGGAATCAGGAAAGAATGAACTGGATAAAGTTGATGAGCCGTATTTTGTGGAAGTTGATCGGTCTTGTTGGGTTTCGGACGAGCACCTTGATATTGCTGAAGTTGTTTTGACAAATTTGAATTTCGGAGAAGGGTTTTCTGGTGATGGATTGAATGGGGATTTTAATCAGGATGATTACTCATTAAGGTTTCGAGTGTGCAACGTGAATGAGTTTATTGGCCGTATGAAGTTAGGCCATTGGCCTGTATTATCCTCTGCTGATATATCTTTGGAATTCATTAAGAAGTGTCCAACAGAGGATACGGGCGAGTGCTCAGTGATTTTATCGGGGAGTTTTGATGGACCGGATGAAGGTATCTCTGGTCTTGTTCACTTGGCAAGTTTGAGGTTTATGACCCTAAGGCCAGTTCGGTGGGTTGTGTCGATTGATATGTCCACCGTCACGGTTAGGGTGGAGATACTTAAGCGTGCATTTGATGCTTGTCAGTCACTTCTGGACATTACTAGGCAAGTCTGGAAAAAAAGTATGAGGAATGTCATGGGTTGGCTTCATCCAGAAGTAATGACATCAGAGGCTAGGTACAGAGTCAGTAAATCAACAGAAATGGAAATTGATTTGCGTACTGAAAATGGATATGCTAATTCAGACCCTAGTAAACTAGCAAGGTTTGATGTCTCTGGGTTCTATGAAGCCATTAAGCGATCAAAAGCAGATGCAATGCTTCAAGCTGACATGCCCGATTTGCTTCCTGAGTTGAAGCCCTATCAGCGCCGTGCAGCTTACTGGATGGTACATAGAGAGAAAGGGTATGCAGAAAGTCCGGTCGAAAAGGGGAACAGTCAGTTCATATCACCTTTATGTTTGCCCTTGGAATTTCTTGACACATGTTCAAAAATGTTCTACAACCCATTCAGTGGAAATGTTTCGTTGCACCCAGAGAATGATTCAGCTTATGTCTGTGGTGGTATTCTTGCTGATGAGATGGGCATGGGGAAAACTGTTGAACTTCTTGCTTGTATATTTGCGCATCCGAAGTCGGCAGATGAAGACAACATATTTGCTGATTCTGAATCTCAAGCTACCGAAGATCAGAAAATCAATCTTAAAAGATTAAAAAGGGAGCGAGTTGAATGTGTATGTGGAGCTGTGAGTGAAAATATGTCATACACAGGATTATGGGTACAGTGTGACATATGCGATGCATGGCAACATGCAGACTGTGTGGGTTATTCAGCTGATGGAAAATCCATCAAATCCAATGAGGCCTCTCAAAAGCACCTGAAGAGGAAAAATACAACCACTGTAGTTGAAAGAGATGGAAAATATATTTGCCAGCTGTGCTCAGAACTGATACAGGCTACTAACTCTCCTGTGGCTACTGCTGCCACTCTTATAATTTGTCCAGCTTCTATTTTGCTCCAATGGCATTCTGAAATTATACGTCATACCCGTGCAGGTTCTTTGAAAACATGTATCTATGAAGGTGTGAGGGAGACTTCTTTTTCAAACGCGTCGGAAATTGATATCAGTGAACTTGTTAGCGCTGACATTGTATTGACAACATATGATGTGCTTAAAGAGGACCTGTCACACGACTCTGATAGGCATGAAGGTGATCGGCGCTTGATGAGATTCCATAAAAGGTACCCTGTTGTTCCTACCGTTCTGACCAGAATATTCTGGTGGAGAGTTTGTTTGGATGAGGCTCAAATGGTGGAGAATAATGCTGCTGCGGCCACAGAAATGGCTATGCGGTTGTATACTAAGCACCGTTGGTGCATTACAGGGACTCCTATACAACGTAAACTTGATGACTTATATGGACTTCTACGATTTCTCAAAGCATGCCCATTTGATGGTTCTAGGTGGTGGGTTGAAGTTATACGAGATCCATATGAGAGGAAAGATGTGGCAGCTATGGAATTTGCACATAAATTTTTCAAGAAAATTATGTGGCGATCTTTAAAAGTGCATGTTGCAGATGAGTTGGAGCTTCCTCCTCAGGAGGAGTGCCTCTCTTGGCTCACTTTATCTCCAATAGAAGAACACTTTTACCAAAGGCAACATGAAACTTGTGTGAGTTATGCCCGAGAAGTCATTGACAGTCTGAAAGATGATATTCGGAAAAGAGAAGTCACAGGATGCTTAGCATCTAATGATTCATCAGATCCACTCATCACACACGCAGAGGCAGGAAAGCTTCTGAATACACTGTTGAAGCTTCGCCAAGCCTGCTGCCACCCTCAAGTAGGAAGCTTTGGGCTTCGTTCTCTGCAACAGTCGCCCATGACTATGGAGGAAATACTAATGGTTCTTGTAGGTAAAACAAAGGTAGAAGGAGAGGAAGCGCTTAGGAGGTCAGTCGTCGCTCTCAATGGGCTTGCCGGGATAGCTATACTCGAACAAAACTTCACCCAAGCTCTATCCTTGTACAAGGAAGCGCTGGATTTAGCTGAAGAGCACAATGAGGATTTCCGCCTGGACCCTTTGTTGAGTATCCACATTTATCATAATCTTGCTGAAATCCTCCCTTTGGCAACAAACTGCTGCCCTTCAAAAGAACAGCTTCCTGGGAGCTCTAGGGAAATGGCCTCCAAGATACAGGGCATTGAAACTTGTGATCAACATGTAGCGAAGAGGCAAAAACTTTGTGGGAAAGAAGAAATTGCCAATGATGCTGGTAACTCACTGGAAGGTACATCTGAATTATCAGATAACGTTCCTGCTGCTCACCAAGAATGCGATAATGGGCCCCAGCCATCATGTAGTTCTTTCAGTGATGCATCTTTGAGAATAGCCTGTGACAATATTAAACAGAAGTACTTGTCTGTATTCAGTTCAAAGCTATCCATGGCTCAACAAGAGTTTAACAAGTCTTACACACAGGTTTGCAATGCAATCAGCGACAGAAAAGATCTAAGTACAGTTTGGTGGTTGGAAGCCTTTCTTCATGCTGAAAAGAACATAGACCTCTCTAGGGATTTGATGAGAAAGATTGAAGAGGCCCTTGTAGGAACTCTAAACAATGGGAAGTCATCGAGAATTGCCTCTCGTTTCCGAAGTATAAGTGGCCTGAAGTATCATGTGCAGACTGGTTTGGACCAATTAGCAGCATCTAGGAAGGCGTTGCTTGATCGACTTTTAGAAATTGATCAGACGATGGAGAAGCCAAAGGAGGAGGATATTCACCGCGTGAGAAACTGTCGAAATTGCAAGGGTTGTGATGATGGTCCTTTGTGTATTCTCTGTGAACTAGATGAATTGTTTCGGGACTATGAAGCAAGGCTCTTTCGAAGTGAAAAAGTAGGCGGAGGGACAGCTACATGTGCTGAAGAGGCTGTGGCTTTGCAGAAGAAGAACTCTGAACTTAATCGTTTTTATCAGAATTTGTCGCAGCTAAATAAAGATTCAACATCATCCAATTCTAATAAAGAGTCAAAGAAGAGAGATGCTGGAAAAGTTGTGGTTTCAAAAGCTCCATCTGAATTAGAGGTTGTTCTTGGAGTTATAAGAAATCATTGCAAGGCCCAATTAGGGAGGGACGGTACATCAGAAGCCACAAAGCACCTCCACGTACTTGAGGGCATGCGCAAGGAGTATCCCCATGCAAGATCCTTGGCAATAGCCCAAGCTCAAATTCTACAAGCTTATGATGAGCTTAACATGGCAACCTCAAGATTGCGCCTGGCAGAAGATGAGGATGATAAAGCTCTTGATGCTTTAAGTGAAGAAGAATTACCTTCAGCTAGCGTCCTATATACTAGTGACAAGTTCACATCCTTGAATTCGTTGTCTGTTATAAAAGGGAAACTTCGTTATTTAAAGGGTCTGGTACAAGCTAAACAAAAGGTGCCGTTGCAAAGTCCAAATCCTTCCTCGGTAGCTGAAGGGCCAGCCACTATTCCAACATCCACAGAACAGAAAAATGTATGCATACCTACAGGCGATAAAGAAACATGCCCGGTTTGTCAAGATCCATTAACCATTCGGAAGATGGTTTTTCCGTGTGGGCACCTTACCTGCTGTAAATGTTTATTTGCAATTACTGAACGGAATAACAAGGTTCAAGATAAGTGGGTAAAATGCCCAACATGTCGACAACATACTGATGTTGGAAACATTGCTTATGCCGACGATGGACAGAGTGAAACTAGTACATCTTCTGTGCTTCATACAACTGAAAGTCGTGAAATGTCTGAAGCATCTGTTATGGTGCAAGGTTCATATGGAACAAAGATTGAAGCAGTGACAAGAAGAATCCTGTGGATAAAGTCTACGGATCCAGAGGCTAAAGTTCTTGTTTTCTCAAGCTGGCATGATGTGCTCGACGTATTGGAGCATGCCTTCACTGCTAATGACATTACGCATGTTCGGATGAAAGGAGGAAGGAAATCACAAGTTGCCATTAGCGAATTTAAAGGCGAAAAGATAAGTGCAAGAGGAAATCACAAGATACGTGGACAGAAAGAGCAAAGATCTTTCCAAGTGTTACTGCTCTTGATTCAACATGGAGCTAACGGCCTCAATCTTCTGGAAGCGAAGCATGTTATTCTTGTCGAGCCGCTTCTCAATCCAGCAGTAGAAGCACAGGCAATCAGCCGGGTCCACCGAATTGGACAGAAAAGCAAAACCATAGCTCATCGTTTCATAGTTAAAGACACGGTCGAAGAGAGTATATATAAACTCAACAAAAGCCGGAACAGCACTGCGTTCATCGGGAACACGAAGAATCAAGATCAGCCTTTCTTCACATTAAAAGACATTGAATCCTTATTCGCAACAGTGCCACCCCCAGCACCAGAAACTCAGGAAAAGGCAACTGAAGTTCCCGACGAGAACCCAACGGAAAGTCTACGGCATTTGCCACCTTCTGTGGCTGCTGCCATAGCAGCTGAGAAGAGACATAAGGAGCAGCATTATTCTTTTGGTCTAAGAGTTGATTTGCGGCTGGTACTAATTCCATGGAAATATCAGAAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0031625 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050896 GO:0051716 GO:0061630 GO:0061659 GO:0070013 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Helicase_C PHD SNF2_N zf-C3HC4

Protein Analysis

1697

Amino Acids

190.38

Weight (kDa)

6.2

Isoelectric Point (pI)

48.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNF2-rel_dom	PF00176	291 - 785	1.1e-64	SNF2-related domain
SHPRH_helical-1st	PF21325	801 - 885	2.1e-23	E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd	PF21324	1011 - 1134	2.1e-10	E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C	PF00271	1450 - 1575	4.5e-07	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003903)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40770 AT2G40770 AT2G40770
fragaria_vesca	FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110 FvH4_7g26110
malus_domestica	MD01G1169300.v1.1
prunus_persica	Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1 Prupe.2G265500_v2.0.a1
pyrus_communis	pycom01g18200
rosa_chinensis	RchiOBHm_Chr1g0372551
rosa_laevigata	RLG00000026892
rosa_multiflora	Rmu_sc0005410.1_g000019 Rmu_sc0011709.1_g000002
rosa_roxburghii	Rroxscaffold_4G00284860
rosa_rugosa	Rorug01G0372700
rosa_samantha	Rh1AG381200 Rh1BG344600 Rh1CG358000 Rh1DG375800
rosa_wichuraiana	Rw1G033520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1502, 3617, 3908
Acc16I	TGCGCA	2 cut(s)	1134, 3698
Acc36I	ACCTGC	2 cut(s)	1553, 4136
Acc65I	GGTACC	1 cut(s)	1752
AccB1I	GGYRCC	3 cut(s)	1752, 3958, 4119
AccB7I	CCANNNNNTGG	2 cut(s)	1022, 1964
AccI	GTMKAC	4 cut(s)	1868, 4204, 4382, 4960
AccII	CGCG	2 cut(s)	1619, 3300
AciI	CCGC	9 cut(s)	22, 26, 1841, 1861, 2584, 3298, 3417, 4670, 5053
AclI	AACGTT	1 cut(s)	2826
AclWI	GGATC	7 cut(s)	27, 1991, 2270, 3714, 4076, 4382, 4395
AcoI	YGGCCR	3 cut(s)	23, 325, 1842
AcuI	CTGAAG	9 cut(s)	225, 1409, 2583, 3203, 3459, 3834, 4014, 4326, 4950
AdeI	CACNNNGTG	1 cut(s)	3301
AfeI	AGCGCT	3 cut(s)	1651, 2457, 2550
AfiI	CCNNNNNNNGG	7 cut(s)	458, 1022, 1964, 2357, 2641, 3085, 3642
AflII	CTTAAG	1 cut(s)	569
AflIII	ACRYGT	6 cut(s)	992, 1577, 1617, 2734, 3431, 4199
AhlI	ACTAGT	2 cut(s)	3869, 4259
AjnI	CCWGG	3 cut(s)	2586, 2677, 3792
AjuI	GAANNNNNNNTTGG	2 cut(s)	3729, 3761
Alw21I	GWGCWC	6 cut(s)	192, 422, 1451, 2571, 2687, 4434
Alw26I	GTCTC	3 cut(s)	787, 1595, 5002
AlwI	GGATC	7 cut(s)	27, 1991, 2270, 3714, 4076, 4382, 4395
Ama87I	CYCGRG	1 cut(s)	2196
Aor51HI	AGCGCT	3 cut(s)	1651, 2457, 2550
ApaI	GGGCCC	1 cut(s)	2862
ArsI	GACNNNNNNTTYG	2 cut(s)	1148, 1180
Asp700I	GAANNNNTTC	4 cut(s)	1030, 2673, 3051, 3372
Asp718I	GGTACC	1 cut(s)	1752
AspLEI	GCGC	8 cut(s)	879, 1135, 1652, 1731, 2458, 2551, 3699, 3792
AsuC2I	CCSGG	3 cut(s)	2494, 4070, 4709
AsuHPI	GGTGA	6 cut(s)	260, 951, 1733, 2512, 2831, 3287
AsuII	TTCGAA	1 cut(s)	3400
AsuNHI	GCTAGC	1 cut(s)	3854
AvaI	CYCGRG	1 cut(s)	2196
AvaII	GGWCC	6 cut(s)	449, 1690, 2590, 3208, 3338, 4711
AxyI	CCTNAGG	2 cut(s)	506, 2109
BaeGI	GKGCMC	2 cut(s)	2862, 4122
BamHI	GGATCC	1 cut(s)	4387
BanI	GGYRCC	3 cut(s)	1752, 3958, 4119
BanII	GRGCYC	2 cut(s)	2687, 2862
BarI	GAAGNNNNNNTAC	2 cut(s)	3828, 3860
BbsI	GAAGAC	1 cut(s)	1161
Bbv12I	GWGCWC	6 cut(s)	192, 422, 1451, 2571, 2687, 4434
BceAI	ACGGC	6 cut(s)	130, 312, 864, 3946, 4642, 4979
BciT130I	CCWGG	3 cut(s)	2588, 2679, 3794
BciVI	GTATCC	3 cut(s)	400, 2615, 3717
BclI	TGATCA	2 cut(s)	2728, 3259
BcnI	CCSGG	3 cut(s)	2494, 4070, 4709
BcoDI	GTCTC	3 cut(s)	787, 1595, 5002
BcuI	ACTAGT	2 cut(s)	3869, 4259
BfmI	CTRYAG	2 cut(s)	1410, 4047
BfoI	RGCGCY	5 cut(s)	880, 1653, 1732, 2459, 2552
BfrI	CTTAAG	1 cut(s)	569
BfuAI	ACCTGC	2 cut(s)	1553, 4136
BfuI	GTATCC	3 cut(s)	400, 2615, 3717
BglI	GCCNNNNNGGC	1 cut(s)	74
BglII	AGATCT	2 cut(s)	3022, 4583
BlpI	GCTNAGC	1 cut(s)	2254
BmcAI	AGTACT	1 cut(s)	2930
Bme1390I	CCNGG	6 cut(s)	2494, 2588, 2679, 3794, 4070, 4709
Bme18I	GGWCC	6 cut(s)	449, 1690, 2590, 3208, 3338, 4711
BmeT110I	CYCGRG	1 cut(s)	2196
BmiI	GGNNCC	8 cut(s)	1754, 2592, 2860, 2861, 3960, 4121, 4389, 4712
BmrFI	CCNGG	6 cut(s)	2494, 2588, 2679, 3794, 4070, 4709
BmtI	GCTAGC	1 cut(s)	3858
BpiI	GAAGAC	1 cut(s)	1161
BplI	GAGNNNNNCTC	2 cut(s)	2108, 2140
Bpu10I	CCTNAGC	1 cut(s)	2459
Bpu1102I	GCTNAGC	1 cut(s)	2254
Bpu14I	TTCGAA	1 cut(s)	3400
BpuEI	CTTGAG	5 cut(s)	1167, 2337, 3707, 3766, 4399
BpuMI	CCSGG	3 cut(s)	2494, 4070, 4709
BsaAI	YACGTR	2 cut(s)	3682, 4565
BsaBI	GATNNNNATC	1 cut(s)	270
BsaJI	CCNNGG	6 cut(s)	975, 2678, 2958, 3723, 3984, 5069
BsaWI	WCCGGW	3 cut(s)	35, 451, 928
Bsc4I	CCNNNNNNNGG	7 cut(s)	458, 1022, 1964, 2357, 2641, 3085, 3642
Bse1I	ACTGG	5 cut(s)	132, 512, 896, 2802, 3205
Bse21I	CCTNAGG	2 cut(s)	506, 2109
Bse3DI	GCAATG	5 cut(s)	70, 831, 3007, 3625, 4227
Bse8I	GATNNNNATC	1 cut(s)	270
BseBI	CCWGG	3 cut(s)	2588, 2679, 3794
BseDI	CCNNGG	6 cut(s)	975, 2678, 2958, 3723, 3984, 5069
BseJI	GATNNNNATC	1 cut(s)	270
BseLI	CCNNNNNNNGG	7 cut(s)	458, 1022, 1964, 2357, 2641, 3085, 3642
BseMI	GCAATG	5 cut(s)	70, 831, 3007, 3625, 4227
BseMII	CTCAG	6 cut(s)	435, 849, 1464, 2123, 4928, 4992
BseNI	ACTGG	5 cut(s)	132, 512, 896, 2802, 3205
BseRI	GAGGAG	3 cut(s)	2097, 2129, 3299
BseSI	GKGCMC	2 cut(s)	2862, 4122
BseX3I	CGGCCG	1 cut(s)	23
BseYI	CCCAGC	2 cut(s)	2862, 4900
BsgI	GTGCAG	3 cut(s)	903, 1581, 3215
Bsh1236I	CGCG	2 cut(s)	1619, 3300
Bsh1285I	CGRYCG	3 cut(s)	26, 933, 4773
BshNI	GGYRCC	3 cut(s)	1752, 3958, 4119
BsiEI	CGRYCG	3 cut(s)	26, 933, 4773
BsiHKAI	GWGCWC	6 cut(s)	192, 422, 1451, 2571, 2687, 4434
BsiHKCI	CYCGRG	1 cut(s)	2196
BsiSI	CCGG	7 cut(s)	36, 452, 929, 2493, 4070, 4708, 4804
BslFI	GGGAC	4 cut(s)	1909, 3392, 3436, 3662
BslI	CCNNNNNNNGG	7 cut(s)	458, 1022, 1964, 2357, 2641, 3085, 3642
BsmAI	GTCTC	3 cut(s)	787, 1595, 5002
BsmFI	GGGAC	4 cut(s)	1909, 3392, 3436, 3662
BsmI	GAATGC	2 cut(s)	1534, 2852
BsoBI	CYCGRG	1 cut(s)	2196
Bsp119I	TTCGAA	1 cut(s)	3400
Bsp120I	GGGCCC	1 cut(s)	2858
Bsp1286I	GDGCHC	8 cut(s)	192, 422, 1451, 2571, 2687, 2862, 4122, 4434
Bsp1407I	TGTACA	1 cut(s)	2538
Bsp1720I	GCTNAGC	1 cut(s)	2254
Bsp19I	CCATGG	2 cut(s)	2958, 5069
BspACI	CCGC	9 cut(s)	22, 26, 1841, 1861, 2584, 3298, 3417, 4670, 5053
BspCNI	CTCAG	6 cut(s)	434, 850, 1463, 2122, 4927, 4993
BspFNI	CGCG	2 cut(s)	1619, 3300
BspLI	GGNNCC	8 cut(s)	1754, 2592, 2860, 2861, 3960, 4121, 4389, 4712
BspMI	ACCTGC	2 cut(s)	1553, 4136
BspOI	GCTAGC	1 cut(s)	3858
BspPI	GGATC	7 cut(s)	27, 1991, 2270, 3714, 4076, 4382, 4395
BspQI	GCTCTTC	1 cut(s)	2559
BspT104I	TTCGAA	1 cut(s)	3400
BspT107I	GGYRCC	3 cut(s)	1752, 3958, 4119
BspTI	CTTAAG	1 cut(s)	569
BsrDI	GCAATG	5 cut(s)	70, 831, 3007, 3625, 4227
BsrGI	TGTACA	1 cut(s)	2538
BsrI	ACTGG	5 cut(s)	132, 512, 896, 2802, 3205
BssECI	CCNNGG	6 cut(s)	975, 2678, 2958, 3723, 3984, 5069
BssNAI	GTATAC	1 cut(s)	1869
BssT1I	CCWWGG	4 cut(s)	975, 2958, 3723, 5069
Bst1107I	GTATAC	1 cut(s)	1869
Bst2UI	CCWGG	3 cut(s)	2588, 2679, 3794
Bst6I	CTCTTC	8 cut(s)	1385, 2559, 2738, 3102, 3435, 3545, 4770, 5000
BstAFI	CTTAAG	1 cut(s)	569
BstAPI	GCANNNNNTGC	2 cut(s)	584, 4429
BstAUI	TGTACA	1 cut(s)	2538
BstBAI	YACGTR	2 cut(s)	3682, 4565
BstBI	TTCGAA	1 cut(s)	3400
BstDSI	CCRYGG	2 cut(s)	2958, 5069
BstENI	CCTNNNNNAGG	1 cut(s)	2355
BstFNI	CGCG	2 cut(s)	1619, 3300
BstH2I	RGCGCY	5 cut(s)	880, 1653, 1732, 2459, 2552
BstHHI	GCGC	8 cut(s)	879, 1135, 1652, 1731, 2458, 2551, 3699, 3792
BstMAI	GTCTC	3 cut(s)	787, 1595, 5002
BstMCI	CGRYCG	3 cut(s)	26, 933, 4773
BstNI	CCWGG	3 cut(s)	2588, 2679, 3794
BstSCI	CCNGG	6 cut(s)	2492, 2586, 2677, 3792, 4068, 4707
BstSFI	CTRYAG	2 cut(s)	1410, 4047
BstSLI	GKGCMC	2 cut(s)	2862, 4122
BstUI	CGCG	2 cut(s)	1619, 3300
BstV2I	GAAGAC	1 cut(s)	1161
BstX2I	RGATCY	7 cut(s)	1996, 2275, 3022, 3719, 4081, 4387, 4583
BstXI	CCANNNNNNTGG	4 cut(s)	519, 1852, 2401, 4721
BstYI	RGATCY	7 cut(s)	1996, 2275, 3022, 3719, 4081, 4387, 4583
BstZ17I	GTATAC	1 cut(s)	1869
BstZI	CGGCCG	1 cut(s)	23
Bsu36I	CCTNAGG	2 cut(s)	506, 2109
BsuI	GTATCC	3 cut(s)	400, 2615, 3717
BtgI	CCRYGG	2 cut(s)	2958, 5069
BtgZI	GCGATG	1 cut(s)	1320
BtsI	GCAGTG	3 cut(s)	4359, 4455, 4812
BveI	ACCTGC	2 cut(s)	1553, 4136
CciNI	GCGGCCGC	1 cut(s)	23
CfoI	GCGC	8 cut(s)	879, 1135, 1652, 1731, 2458, 2551, 3699, 3792
CseI	GACGC	2 cut(s)	1608, 3847
CspCI	CAANNNNNGTGG	6 cut(s)	1396, 1431, 4163, 4198, 4546, 4581
DraI	TTTAAA	3 cut(s)	2073, 3930, 4525
DraIII	CACNNNGTG	1 cut(s)	3301
EaeI	YGGCCR	3 cut(s)	23, 325, 1842
EagI	CGGCCG	1 cut(s)	23
Eam1104I	CTCTTC	8 cut(s)	1385, 2559, 2738, 3102, 3435, 3545, 4770, 5000
EarI	CTCTTC	8 cut(s)	1385, 2559, 2738, 3102, 3435, 3545, 4770, 5000
EciI	GGCGGA	2 cut(s)	2573, 3432
Ecl136II	GAGCTC	1 cut(s)	2685
EclXI	CGGCCG	1 cut(s)	23
Eco130I	CCWWGG	4 cut(s)	975, 2958, 3723, 5069
Eco147I	AGGCCT	1 cut(s)	1373
Eco24I	GRGCYC	2 cut(s)	2687, 2862
Eco32I	GATATC	1 cut(s)	1633
Eco47I	GGWCC	6 cut(s)	449, 1690, 2590, 3208, 3338, 4711
Eco47III	AGCGCT	3 cut(s)	1651, 2457, 2550
Eco52I	CGGCCG	1 cut(s)	23
Eco53kI	GAGCTC	1 cut(s)	2685
Eco57I	CTGAAG	9 cut(s)	225, 1409, 2583, 3203, 3459, 3834, 4014, 4326, 4950
Eco81I	CCTNAGG	2 cut(s)	506, 2109
Eco88I	CYCGRG	1 cut(s)	2196
EcoICRI	GAGCTC	1 cut(s)	2685
EcoNI	CCTNNNNNAGG	1 cut(s)	2355
EcoO109I	RGGNCCY	3 cut(s)	1690, 2859, 3112
EcoRI	GAATTC	2 cut(s)	380, 3892
EcoRII	CCWGG	3 cut(s)	2586, 2677, 3792
EcoRV	GATATC	1 cut(s)	1633
EcoT14I	CCWWGG	4 cut(s)	975, 2958, 3723, 5069
EcoT22I	ATGCAT	3 cut(s)	1312, 2893, 4043
EcoT38I	GRGCYC	2 cut(s)	2687, 2862
ErhI	CCWWGG	4 cut(s)	975, 2958, 3723, 5069
FalI	AAGNNNNNCTT	8 cut(s)	2658, 2690, 3039, 3071, 3099, 3131, 3903, 3935
FaqI	GGGAC	4 cut(s)	1909, 3392, 3436, 3662
FauNDI	CATATG	4 cut(s)	1301, 1672, 2002, 4333
FbaI	TGATCA	2 cut(s)	2728, 3259
FblI	GTMKAC	4 cut(s)	1868, 4204, 4382, 4960
FriOI	GRGCYC	2 cut(s)	2687, 2862
FspI	TGCGCA	2 cut(s)	1134, 3698
GlaI	GCGC	8 cut(s)	878, 1134, 1651, 1730, 2457, 2550, 3698, 3791
GsaI	CCCAGC	2 cut(s)	2866, 4904
HaeII	RGCGCY	5 cut(s)	880, 1653, 1732, 2459, 2552
HapII	CCGG	7 cut(s)	36, 452, 929, 2493, 4070, 4708, 4804
HgaI	GACGC	2 cut(s)	1608, 3847
HhaI	GCGC	8 cut(s)	879, 1135, 1652, 1731, 2458, 2551, 3699, 3792
Hin6I	GCGC	8 cut(s)	877, 1133, 1650, 1729, 2456, 2549, 3697, 3790
HinP1I	GCGC	8 cut(s)	877, 1133, 1650, 1729, 2456, 2549, 3697, 3790
HincII	GTYRAC	1 cut(s)	4205
HindII	GTYRAC	1 cut(s)	4205
HindIII	AAGCTT	4 cut(s)	2306, 2327, 2362, 3753
HpaII	CCGG	7 cut(s)	36, 452, 929, 2493, 4070, 4708, 4804
HphI	GGTGA	6 cut(s)	260, 951, 1733, 2512, 2831, 3287
Hpy99I	CGWCG	5 cut(s)	1624, 2477, 4244, 4439, 4943
HpyCH4IV	ACGT	7 cut(s)	309, 1549, 1909, 2826, 3681, 4437, 4564
HpySE526I	ACGT	7 cut(s)	309, 1549, 1909, 2826, 3681, 4437, 4564
HspAI	GCGC	8 cut(s)	877, 1133, 1650, 1729, 2456, 2549, 3697, 3790
KpnI	GGTACC	1 cut(s)	1756
Ksp22I	TGATCA	2 cut(s)	2728, 3259
LguI	GCTCTTC	1 cut(s)	2559
LmnI	GCTCC	9 cut(s)	1228, 1250, 1530, 2098, 2682, 3589, 4444, 4619, 5017
MaeII	ACGT	7 cut(s)	309, 1549, 1909, 2826, 3681, 4437, 4564
MflI	RGATCY	7 cut(s)	1996, 2275, 3022, 3719, 4081, 4387, 4583
MhlI	GDGCHC	8 cut(s)	192, 422, 1451, 2571, 2687, 2862, 4122, 4434
MluI	ACGCGT	1 cut(s)	1617
MlyI	GAGTC	5 cut(s)	64, 702, 1697, 1891, 3551
MmeI	TCCRAC	6 cut(s)	422, 1602, 2076, 3024, 4037, 4201
Mph1103I	ATGCAT	3 cut(s)	1312, 2893, 4043
MroXI	GAANNNNTTC	4 cut(s)	1030, 2673, 3051, 3372
MslI	CAYNNNNRTG	6 cut(s)	964, 1557, 1719, 1850, 2399, 4215
MspA1I	CMGCKG	3 cut(s)	1343, 1444, 5000
MspCI	CTTAAG	1 cut(s)	569
MspI	CCGG	7 cut(s)	36, 452, 929, 2493, 4070, 4708, 4804
MspR9I	CCNGG	6 cut(s)	2494, 2588, 2679, 3794, 4070, 4709
Mva1269I	GAATGC	2 cut(s)	1534, 2852
MvaI	CCWGG	3 cut(s)	2588, 2679, 3794
MvnI	CGCG	2 cut(s)	1619, 3300
NciI	CCSGG	3 cut(s)	2494, 4070, 4709
NcoI	CCATGG	2 cut(s)	2958, 5069
NdeI	CATATG	4 cut(s)	1301, 1672, 2002, 4333
NheI	GCTAGC	1 cut(s)	3854
NlaIV	GGNNCC	8 cut(s)	1754, 2592, 2860, 2861, 3960, 4121, 4389, 4712
NmuCI	GTSAC	8 cut(s)	547, 594, 1295, 1695, 2242, 2909, 3872, 4354
NotI	GCGGCCGC	1 cut(s)	23
NsbI	TGCGCA	2 cut(s)	1134, 3698
NsiI	ATGCAT	3 cut(s)	1312, 2893, 4043
NspV	TTCGAA	1 cut(s)	3400
PaeI	GCATGC	3 cut(s)	1956, 3697, 4451
PceI	AGGCCT	1 cut(s)	1373
PciI	ACATGT	5 cut(s)	992, 1577, 2734, 3431, 4199
PciSI	GCTCTTC	1 cut(s)	2559
PcsI	WCGNNNNNNNCGW	1 cut(s)	1555
PctI	GAATGC	2 cut(s)	1534, 2852
PdmI	GAANNNNTTC	4 cut(s)	1030, 2673, 3051, 3372
PflFI	GACNNNGTC	1 cut(s)	4769
PflMI	CCANNNNNTGG	2 cut(s)	1022, 1964
PleI	GAGTC	5 cut(s)	63, 701, 1697, 1891, 3550
PpsI	GAGTC	5 cut(s)	63, 701, 1697, 1891, 3550
Ppu21I	YACGTR	2 cut(s)	3682, 4565
PpuMI	RGGWCCY	1 cut(s)	1690
PscI	ACATGT	5 cut(s)	992, 1577, 2734, 3431, 4199
PsiI	TTATAA	3 cut(s)	1502, 3617, 3908
Psp124BI	GAGCTC	1 cut(s)	2687
Psp1406I	AACGTT	1 cut(s)	2826
Psp5II	RGGWCCY	1 cut(s)	1690
Psp6I	CCWGG	3 cut(s)	2586, 2677, 3792
PspFI	CCCAGC	2 cut(s)	2862, 4900
PspGI	CCWGG	3 cut(s)	2586, 2677, 3792
PspN4I	GGNNCC	8 cut(s)	1754, 2592, 2860, 2861, 3960, 4121, 4389, 4712
PspOMI	GGGCCC	1 cut(s)	2858
PspPPI	RGGWCCY	1 cut(s)	1690
PsuI	RGATCY	7 cut(s)	1996, 2275, 3022, 3719, 4081, 4387, 4583
PsyI	GACNNNGTC	1 cut(s)	4769
PvuII	CAGCTG	3 cut(s)	1343, 1444, 5000
RseI	CAYNNNNRTG	6 cut(s)	964, 1557, 1719, 1850, 2399, 4215
SacI	GAGCTC	1 cut(s)	2687
SalI	GTCGAC	1 cut(s)	4203
SapI	GCTCTTC	1 cut(s)	2559
ScaI	AGTACT	1 cut(s)	2930
SchI	GAGTC	5 cut(s)	64, 702, 1697, 1891, 3551
ScrFI	CCNGG	6 cut(s)	2494, 2588, 2679, 3794, 4070, 4709
SduI	GDGCHC	8 cut(s)	192, 422, 1451, 2571, 2687, 2862, 4122, 4434
SfcI	CTRYAG	2 cut(s)	1410, 4047
SfuI	TTCGAA	1 cut(s)	3400
SinI	GGWCC	6 cut(s)	449, 1690, 2590, 3208, 3338, 4711
SmiMI	CAYNNNNRTG	6 cut(s)	964, 1557, 1719, 1850, 2399, 4215
SmlI	CTYRAG	6 cut(s)	569, 1182, 2352, 3686, 3781, 4414
SmoI	CTYRAG	6 cut(s)	569, 1182, 2352, 3686, 3781, 4414
SpeI	ACTAGT	2 cut(s)	3869, 4259
SphI	GCATGC	3 cut(s)	1956, 3697, 4451
SseBI	AGGCCT	1 cut(s)	1373
SsiI	CCGC	9 cut(s)	22, 26, 1841, 1861, 2584, 3298, 3417, 4670, 5053
SspI	AATATT	2 cut(s)	1785, 2917
SstI	GAGCTC	1 cut(s)	2687
StuI	AGGCCT	1 cut(s)	1373
StyD4I	CCNGG	6 cut(s)	2492, 2586, 2677, 3792, 4068, 4707
StyI	CCWWGG	4 cut(s)	975, 2958, 3723, 5069
TaiI	ACGT	7 cut(s)	312, 1552, 1912, 2829, 3684, 4440, 4567
TaqII	GACCGA	1 cut(s)	156
TatI	WGTACW	4 cut(s)	2538, 2928, 3029, 4262
TauI	GCSGC	5 cut(s)	25, 28, 1844, 4672, 5056
TseFI	GTSAC	8 cut(s)	547, 594, 1295, 1695, 2242, 2909, 3872, 4354
Tsp45I	GTSAC	8 cut(s)	547, 594, 1295, 1695, 2242, 2909, 3872, 4354
TspGWI	ACGGA	4 cut(s)	4101, 4174, 4400, 4967
Tth111I	GACNNNGTC	1 cut(s)	4769
Van91I	CCANNNNNTGG	2 cut(s)	1022, 1964
Vha464I	CTTAAG	1 cut(s)	569
VpaK11BI	GGWCC	6 cut(s)	449, 1690, 2590, 3208, 3338, 4711
XagI	CCTNNNNNAGG	1 cut(s)	2355
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4099
XmiI	GTMKAC	4 cut(s)	1868, 4204, 4382, 4960
XmnI	GAANNNNTTC	4 cut(s)	1030, 2673, 3051, 3372
ZrmI	AGTACT	1 cut(s)	2930
Zsp2I	ATGCAT	3 cut(s)	1312, 2893, 4043

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.