pycom01g18880

Ceramide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005461
Physical Location & Seq
Reverse (-)
17693393 .. 17696237
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGGATTTCTTTCTTCAAGGCATAAAGCTCCATACCCGCCAACTCCTTCAGAACATTGAGCAGTCTCCTCTCCTCCCAAGTCCAATCCATGATGGTTCGGGAGCCACAGTTATCAATGAAGATCCAGAGTTTCCCTTCCCCAATGAGCATTTCAGATTTGGAATCATTCCTGCTGGCTCTACCGATGCCATTGTGATGTGCACAACTGGTGCTCGAGATCCTATAACATCTACGTTTCACATTGTCCTTGGGAAAAGGGTTTCCCTTGATGTAGCCCAAGTTGTAAGGTGGAAAACAACATCTACATCAAAGGTTGAACCTTTTGTGCGCTATGCAGCGTCTTTTGCCGGGTACGGATTTTATGGAGATGTCATTACTGAGAGTGAAAAATACCGTTGGATGGGTCCAAAAAGATATGATTATGCAGGAACAAGGGTATTTTTAAAGCACCGGTCCTATGAGGCGGAAGTAGCATATATAGAAGTCAAACCAGAAGGAAAGTCAATTCCTGAAAGAGGAAGTTTGAGCGGTCGCAAGCGACCATTTTGGAGCCCAAATAAATCCGATCAAGCAATGTGCCGTGTTAACTGCAAGGTCTGTAGCAGCACCACCCCTTGCTCATATCCAGGAGAAACAAAATGGTCCATATCAAAGGGAAAGTTTCTTAGCATTGGTGCTGCAGTAATTTCCAATAGAAATGAAAGAGCACCTGACGGTTTGGTGGCTGATGCACACCTTTCAGACGGTTTCATGCATCTCATATCGATCAAAGACTGCTCTCATGCTCTCTATCTATGGCATCTTACCCAGCTTGCAAAGAAGGGCGGAAACCCCTTCGATTTCAAGTTTGTTGAACATCACAAGACCACTGCTTTTACGTTCACGTCTTCCGGCAACGAGAGTGTATGGAATTTGGACGGGGAACTCTTTCAAGCACACCAATTATCGGCGCAAGTATTTCGAGGCCTTGTTAGCTTGTTTGCCTCCGGTCCTGAAATTTGA

Protein Analysis

334

Amino Acids

37.11

Weight (kDa)

8.63

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CERK_C PF19280 116 - 291 4.8e-13 Ceramide kinase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 528
AciI CCGC 4 cut(s) 37, 464, 528, 825
AclWI GGATC 2 cut(s) 116, 212
AcsI RAATTY 2 cut(s) 910, 996
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 400, 515, 946
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 5 cut(s) 17, 317, 844, 854, 932
AjiI CACGTC 1 cut(s) 885
AjnI CCWGG 1 cut(s) 625
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AluBI AGCT 3 cut(s) 28, 811, 975
AluI AGCT 3 cut(s) 28, 811, 975
Alw21I GWGCWC 3 cut(s) 203, 214, 709
Alw26I GTCTC 1 cut(s) 69
Alw44I GTGCAC 1 cut(s) 199
AlwI GGATC 2 cut(s) 116, 212
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 1 cut(s) 964
ApaLI GTGCAC 1 cut(s) 199
ApeKI GCWGC 3 cut(s) 335, 603, 677
ApoI RAATTY 2 cut(s) 910, 996
AsiGI ACCGGT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 927
AspLEI GCGC 2 cut(s) 330, 952
AspS9I GGNCC 4 cut(s) 404, 453, 642, 989
AsuC2I CCSGG 1 cut(s) 349
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 4 cut(s) 404, 453, 642, 989
BaeGI GKGCMC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 879
Bbv12I GWGCWC 3 cut(s) 203, 214, 709
BbvI GCAGC 3 cut(s) 347, 615, 664
BccI CCATC 2 cut(s) 86, 394
BceAI ACGGC 1 cut(s) 564
BcgI CGANNNNNNTGC 2 cut(s) 941, 975
BciT130I CCWGG 1 cut(s) 627
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 598, 678
BisI GCNGC 3 cut(s) 336, 604, 678
BlsI GCNGC 3 cut(s) 337, 605, 679
Bme1390I CCNGG 2 cut(s) 349, 627
Bme18I GGWCC 4 cut(s) 404, 453, 642, 989
BmeT110I CYCGRG 1 cut(s) 213
BmgBI CACGTC 1 cut(s) 885
BmgT120I GGNCC 4 cut(s) 404, 453, 642, 989
BmiI GGNNCC 3 cut(s) 103, 405, 551
BmrFI CCNGG 2 cut(s) 349, 627
BmsI GCATC 4 cut(s) 175, 718, 763, 808
BpiI GAAGAC 1 cut(s) 879
BpuMI CCSGG 1 cut(s) 349
Bsa29I ATCGAT 1 cut(s) 764
BsaJI CCNNGG 1 cut(s) 247
BsaWI WCCGGW 2 cut(s) 450, 986
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 3 cut(s) 400, 515, 946
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 1 cut(s) 211
Bse3DI GCAATG 1 cut(s) 579
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 764
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 1 cut(s) 405
BseLI CCNNNNNNNGG 3 cut(s) 400, 515, 946
BseMI GCAATG 1 cut(s) 579
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 211
BseRI GAGGAG 2 cut(s) 57, 62
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 3 cut(s) 347, 615, 664
BseYI CCCAGC 1 cut(s) 807
Bsh1285I CGRYCG 1 cut(s) 532
BshFI GGCC 1 cut(s) 966
BshTI ACCGGT 1 cut(s) 450
BshVI ATCGAT 1 cut(s) 764
BsiEI CGRYCG 1 cut(s) 532
BsiHKAI GWGCWC 3 cut(s) 203, 214, 709
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 4 cut(s) 348, 451, 891, 987
BslI CCNNNNNNNGG 3 cut(s) 400, 515, 946
BsmAI GTCTC 1 cut(s) 69
BsnI GGCC 1 cut(s) 966
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 4 cut(s) 203, 214, 554, 709
Bsp143I GATC 4 cut(s) 121, 217, 565, 765
BspACI CCGC 4 cut(s) 37, 464, 528, 825
BspANI GGCC 1 cut(s) 966
BspCNI CTCAG 1 cut(s) 370
BspDI ATCGAT 1 cut(s) 764
BspLI GGNNCC 3 cut(s) 103, 405, 551
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 2 cut(s) 116, 212
BsrBI CCGCTC 1 cut(s) 528
BsrDI GCAATG 1 cut(s) 579
BsrFI RCCGGY 1 cut(s) 450
BsrI ACTGG 1 cut(s) 211
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 4 cut(s) 121, 217, 565, 765
BssT1I CCWWGG 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 4 cut(s) 109, 395, 716, 746
BstC8I GCNNGC 3 cut(s) 175, 536, 813
BstDEI CTNAG 2 cut(s) 378, 665
BstENI CCTNNNNNAGG 1 cut(s) 513
BstF5I GGATG 1 cut(s) 405
BstHHI GCGC 2 cut(s) 330, 952
BstKTI GATC 4 cut(s) 124, 220, 568, 768
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 4 cut(s) 121, 217, 565, 765
BstMCI CGRYCG 1 cut(s) 532
BstMWI GCNNNNNNNGC 3 cut(s) 344, 470, 972
BstNI CCWGG 1 cut(s) 627
BstSCI CCNGG 2 cut(s) 347, 625
BstSFI CTRYAG 2 cut(s) 598, 678
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 347, 615, 664
BstV2I GAAGAC 1 cut(s) 879
BstX2I RGATCY 2 cut(s) 121, 217
BstYI RGATCY 2 cut(s) 121, 217
Bsu15I ATCGAT 1 cut(s) 764
BsuRI GGCC 1 cut(s) 966
BsuTUI ATCGAT 1 cut(s) 764
BtrI CACGTC 1 cut(s) 885
BtsCI GGATG 1 cut(s) 405
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 1 cut(s) 867
Cac8I GCNNGC 3 cut(s) 175, 536, 813
CfoI GCGC 2 cut(s) 330, 952
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 4 cut(s) 404, 453, 642, 989
ClaI ATCGAT 1 cut(s) 764
CseI GACGC 1 cut(s) 327
Csp6I GTAC 1 cut(s) 352
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 3 cut(s) 89, 751, 782
CviJI RGCY 9 cut(s) 28, 104, 177, 275, 552, 725, 811, 966, 975
CviKI_1 RGCY 9 cut(s) 28, 104, 177, 275, 552, 725, 811, 966, 975
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 2 cut(s) 378, 665
DpnI GATC 4 cut(s) 123, 219, 567, 767
DpnII GATC 4 cut(s) 121, 217, 565, 765
DraI TTTAAA 1 cut(s) 444
EciI GGCGGA 2 cut(s) 479, 840
Eco130I CCWWGG 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 554
Eco47I GGWCC 4 cut(s) 404, 453, 642, 989
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 247
FaeI CATG 3 cut(s) 92, 754, 785
FatI CATG 3 cut(s) 88, 750, 781
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 3 cut(s) 336, 604, 678
FokI GGATG 1 cut(s) 412
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 3 cut(s) 336, 604, 678
GlaI GCGC 2 cut(s) 329, 951
GluI GCNGC 3 cut(s) 336, 604, 678
GsaI CCCAGC 1 cut(s) 811
HaeIII GGCC 1 cut(s) 966
HapII CCGG 4 cut(s) 348, 451, 891, 987
HgaI GACGC 1 cut(s) 327
HhaI GCGC 2 cut(s) 330, 952
Hin1II CATG 3 cut(s) 92, 754, 785
Hin6I GCGC 2 cut(s) 328, 950
HinP1I GCGC 2 cut(s) 328, 950
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 1 cut(s) 162
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 4 cut(s) 348, 451, 891, 987
Hpy166II GTNNAC 3 cut(s) 201, 586, 882
Hpy188I TCNGA 4 cut(s) 51, 155, 565, 742
Hpy188III TCNNGA 5 cut(s) 99, 125, 215, 509, 992
Hpy8I GTNNAC 3 cut(s) 201, 586, 882
HpyAV CCTTC 5 cut(s) 56, 145, 488, 814, 844
HpyCH4III ACNGT 4 cut(s) 109, 395, 716, 746
HpyCH4IV ACGT 3 cut(s) 233, 878, 884
HpyCH4V TGCA 8 cut(s) 201, 335, 425, 591, 680, 731, 754, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 470, 972
HpyF3I CTNAG 2 cut(s) 378, 665
HpySE526I ACGT 3 cut(s) 233, 878, 884
Hsp92II CATG 3 cut(s) 92, 754, 785
HspAI GCGC 2 cut(s) 328, 950
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 4 cut(s) 121, 217, 565, 765
LmnI GCTCC 3 cut(s) 33, 101, 549
Lsp1109I GCAGC 3 cut(s) 347, 615, 664
LweI GCATC 4 cut(s) 175, 718, 763, 808
MaeII ACGT 3 cut(s) 233, 878, 884
MalI GATC 4 cut(s) 123, 219, 567, 767
MbiI CCGCTC 1 cut(s) 528
MboI GATC 4 cut(s) 121, 217, 565, 765
MboII GAAGA 3 cut(s) 5, 131, 879
MflI RGATCY 2 cut(s) 121, 217
MhlI GDGCHC 4 cut(s) 203, 214, 554, 709
MluCI AATT 5 cut(s) 504, 684, 910, 941, 996
MmeI TCCRAC 1 cut(s) 377
MnlI CCTC 6 cut(s) 78, 83, 454, 509, 956, 994
Mph1103I ATGCAT 1 cut(s) 756
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 2 cut(s) 443, 585
MslI CAYNNNNRTG 1 cut(s) 194
MspI CCGG 4 cut(s) 348, 451, 891, 987
MspR9I CCNGG 2 cut(s) 349, 627
MvaI CCWGG 1 cut(s) 627
MwoI GCNNNNNNNGC 3 cut(s) 344, 470, 972
NciI CCSGG 1 cut(s) 349
NdeII GATC 4 cut(s) 121, 217, 565, 765
NlaIII CATG 3 cut(s) 92, 754, 785
NlaIV GGNNCC 3 cut(s) 103, 405, 551
NsiI ATGCAT 1 cut(s) 756
PaeR7I CTCGAG 1 cut(s) 213
PceI AGGCCT 1 cut(s) 966
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 1 cut(s) 162
PfoI TCCNGGA 1 cut(s) 625
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 3 cut(s) 337, 605, 679
Psp6I CCWGG 1 cut(s) 625
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 3 cut(s) 103, 405, 551
PspPI GGNCC 4 cut(s) 404, 453, 642, 989
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 2 cut(s) 121, 217
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 2 cut(s) 443, 585
SatI GCNGC 3 cut(s) 336, 604, 678
Sau3AI GATC 4 cut(s) 121, 217, 565, 765
Sau96I GGNCC 4 cut(s) 404, 453, 642, 989
ScrFI CCNGG 2 cut(s) 349, 627
SduI GDGCHC 4 cut(s) 203, 214, 554, 709
SfaNI GCATC 4 cut(s) 175, 718, 763, 808
SfcI CTRYAG 2 cut(s) 598, 678
Sfr274I CTCGAG 1 cut(s) 213
SinI GGWCC 4 cut(s) 404, 453, 642, 989
SlaI CTCGAG 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 5 cut(s) 504, 684, 910, 941, 996
SseBI AGGCCT 1 cut(s) 966
SsiI CCGC 4 cut(s) 37, 464, 528, 825
StuI AGGCCT 1 cut(s) 966
StyD4I CCNGG 2 cut(s) 347, 625
StyI CCWWGG 1 cut(s) 247
TaaI ACNGT 4 cut(s) 109, 395, 716, 746
TaiI ACGT 3 cut(s) 236, 881, 887
TaqI TCGA 4 cut(s) 214, 764, 837, 961
TasI AATT 5 cut(s) 504, 684, 910, 941, 996
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 2 cut(s) 443, 585
Tru9I TTAA 2 cut(s) 443, 585
TscAI CASTG 1 cut(s) 874
TseI GCWGC 3 cut(s) 335, 603, 677
TspDTI ATGAA 3 cut(s) 132, 714, 739
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 1 cut(s) 874
VneI GTGCAC 1 cut(s) 199
VpaK11BI GGWCC 4 cut(s) 404, 453, 642, 989
XagI CCTNNNNNAGG 1 cut(s) 513
XapI RAATTY 2 cut(s) 910, 996
XhoI CTCGAG 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 927
Zsp2I ATGCAT 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.