pycom01g21010

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014806
Physical Location & Seq
Reverse (-)
19239470 .. 19241242
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21010.1

Sequence Viewer

Length: 1623 bp
ATGCGAGGTTCCTTAGAGTTCACTCTCGCGAAACTATTACCACAATGCACGTTCAAACAGTTGAAGCAAATCCATGCCCTCATTGTCACAACCTCTATCAACCAGAACATCCAAATTTTCTCAAAATTTCTCCGGCGGAGCACCGAGTTTGGATCCATGGAGTATCCCTATCTCATATTTTCCCAACTGGGTCCTCAACTCAGTAACGACATTGTGATTCAGAACGCCATGATCAGAGGGTACGATATCAACGGTCCGTTCGAGCAATGCGTCTCAGTGTTTGATGAAATGCCTCTGAGAGGAATCAAACCCCATAACTTCACCTACCCATATGTCTTGAACTCCTGCTCTAAACTGGGTTGGTACGGAAAAGGAAAACAAGTGCATTGCCATATTGTAAAGCATGGGTTCGAGTCAAATCTGGCGGTTGCTAATGCCCTTTTCAATATGTATGTTAAAATGCTGGATTCCTGCGACTCTGGTGTTGTAAATTATAGGACTTTGAATGATGCCCGCAAGGTGTTCGATTATATGCGTGTGAAACCTGTAGAATTGTGGAACCGCATGATCTCACGGTATGCGAGCGTTAGTGATGTTCATAGTGCGAGAAAGTTGTTTGATGATATGCCTGACAAGGACGTTGTTTCATGGAATTCTATGATTTCCGGTTATGCTGCAGTAAGAGATGTAGGCAGTGCAAGCGATCTGTTTGAGAGGATGCCGGAAAAGGATGTCATCTCGTGGACATTGATGATCAGAGTCATAGACAATATGAAGAAGCATTGGACGTTTTTGTGCAAATGCATTCGGAAGGTGTGGTTCCGGATGGATATACATTTGTTTCGGTTCTGTCACTTGATAGAAGATTGGTCTCGTTTCGGACCCATTTCAGGGACTGCCCTTATCGACATGTATGCGAAGTGCGGGGACATAAACAGAGCGTTTGCTCTTTTTATCAAGATAGGAAAGAGGGATGTATTTTGTTGGAATGTTATGATTAGATCGGTCGCTATCCATGGAAGAGCAGAAGATGCCGTCAAGATATACTCATTGATGCAGAAGGAAGGGTTGAACCCGAATGACTTCACGTTCACAGCTGCTCTCTTTGCTTGTAGCCATGGAGGCTTGGTAGAAGAAGGCCAAAGAATCTTCCATTCCATGGACAAAGAATTTGCGATTGTGCCAAAGCTTGAGCATTTTGGCTGCCTAATCGATTTGCTGAGCCGAAATGGTCGGGTGGAGGAAGCACTACGTATGGCAAAGGAAATGCCGTATAACCCCGACATTGCTATATGGGGAACCTTGCTTGGGGGTTGCAGAGAAACTAGTCACCTTAAGTTGGCAGAGGAAGTGATAGAGAAGGTTACTGAGTTGGAAAGAGACGAATCCGGGGTTTATGTCCTCTTGGCTAACATGCGTGCCTCGGCGGGGCAATGGCCAGAGGCTCAAAGTGCAAGAGAGAGAATGGAAGAAAAGAAGATTTGGAAGAACACAGGACGCAGTCATGTTTATGCCTCTAATGGAACCCAGGAGCCCGAGATCGAAAGCCACTCAAAATATGGAGAGGAGAAGGTAGAACAAATTAGATTCACTTCAAACCAATGTGAGCCTAAAAAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

541

Amino Acids

62.09

Weight (kDa)

8.27

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 70 - 118 9.1e-08 PPR repeat family
PPR_2 PF13041 212 - 245 4.6e-06 PPR repeat family
PPR PF01535 215 - 244 2.8e-06 PPR repeat
PPR_2 PF13041 324 - 371 2.1e-10 PPR repeat family
E_motif PF20431 444 - 503 3.4e-08 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014806)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g29410
malus_domestica MD01G1200000.v1.1 MD07G1267900.v1.1
prunus_persica Prupe.2G293600_v2.0.a1
pyrus_communis pycom01g21010 pycom07g24550
rosa_chinensis RchiOBHm_Chr1g0377181 RchiOBHm_Chr1g0377191
rosa_laevigata RLG00000026538
rosa_multiflora Rmu_ssc0000155.1_g000013 Rmu_ssc0000155.1_g000014
rosa_roxburghii Rroxscaffold_4G00281400
rosa_rugosa Rorug01G0401900
rosa_samantha Rh1AG419300 Rh1BG378400 Rh1CG392400
rosa_wichuraiana Rw1G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1159
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 822
AciI CCGC 6 cut(s) 136, 425, 514, 562, 924, 1427
AclWI GGATC 2 cut(s) 147, 160
AcoI YGGCCR 1 cut(s) 1436
AcsI RAATTY 4 cut(s) 114, 125, 652, 1169
AfaI GTAC 2 cut(s) 242, 365
AfiI CCNNNNNNNGG 7 cut(s) 299, 890, 891, 1159, 1308, 1339, 1428
AflII CTTAAG 1 cut(s) 1334
AflIII ACRYGT 1 cut(s) 909
AgsI TTSAA 7 cut(s) 55, 64, 340, 445, 505, 1072, 1596
AhlI ACTAGT 1 cut(s) 1325
AjnI CCWGG 1 cut(s) 1527
AluBI AGCT 2 cut(s) 1097, 1189
AluI AGCT 2 cut(s) 1097, 1189
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 3 cut(s) 277, 876, 1374
AlwI GGATC 2 cut(s) 147, 160
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 1 cut(s) 1535
Aor13HI TCCGGA 1 cut(s) 822
AoxI GGCC 2 cut(s) 1138, 1436
ApeKI GCWGC 3 cut(s) 674, 1097, 1203
ApoI RAATTY 4 cut(s) 114, 125, 652, 1169
ArsI GACNNNNNNTTYG 2 cut(s) 1154, 1186
Asp700I GAANNNNTTC 1 cut(s) 1082
AspS9I GGNCC 3 cut(s) 191, 254, 881
AsuC2I CCSGG 1 cut(s) 1390
AsuHPI GGTGA 2 cut(s) 313, 1322
AvaI CYCGRG 1 cut(s) 1535
AvaII GGWCC 3 cut(s) 191, 254, 881
BalI TGGCCA 1 cut(s) 1438
BamHI GGATCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 1536
BauI CACGAG 1 cut(s) 739
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 3 cut(s) 661, 1084, 1190
BccI CCATC 1 cut(s) 820
BceAI ACGGC 2 cut(s) 1019, 1255
BcgI CGANNNNNNTGC 4 cut(s) 505, 539, 896, 930
BciT130I CCWGG 1 cut(s) 1529
BciVI GTATCC 1 cut(s) 174
BclI TGATCA 2 cut(s) 231, 753
BcnI CCSGG 1 cut(s) 1390
BcoDI GTCTC 3 cut(s) 277, 876, 1374
BcuI ACTAGT 1 cut(s) 1325
BfaI CTAG 1 cut(s) 1326
BfmI CTRYAG 2 cut(s) 546, 675
BfrI CTTAAG 1 cut(s) 1334
BfuI GTATCC 1 cut(s) 174
BglI GCCNNNNNGGC 1 cut(s) 1122
BisI GCNGC 3 cut(s) 675, 1098, 1204
BlpI GCTNAGC 1 cut(s) 1220
BlsI GCNGC 3 cut(s) 676, 1099, 1205
Bme1390I CCNGG 2 cut(s) 1390, 1529
Bme18I GGWCC 3 cut(s) 191, 254, 881
BmeT110I CYCGRG 1 cut(s) 1535
BmgT120I GGNCC 3 cut(s) 191, 254, 881
BmiI GGNNCC 9 cut(s) 10, 154, 192, 560, 821, 883, 1300, 1525, 1533
BmrFI CCNGG 2 cut(s) 1390, 1529
BmrI ACTGGG 2 cut(s) 197, 365
BmsI GCATC 4 cut(s) 499, 708, 1021, 1044
BmuI ACTGGG 2 cut(s) 197, 365
Bpu1102I GCTNAGC 1 cut(s) 1220
BpuEI CTTGAG 1 cut(s) 1211
BpuMI CCSGG 1 cut(s) 1390
Bsa29I ATCGAT 1 cut(s) 1212
BsaAI YACGTR 1 cut(s) 1253
BsaI GGTCTC 1 cut(s) 876
BsaJI CCNNGG 7 cut(s) 156, 1015, 1117, 1158, 1389, 1422, 1527
BsaWI WCCGGW 2 cut(s) 665, 822
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bsc4I CCNNNNNNNGG 7 cut(s) 299, 890, 891, 1159, 1308, 1339, 1428
Bse1I ACTGG 2 cut(s) 192, 360
Bse3DI GCAATG 4 cut(s) 272, 385, 1284, 1439
BseAI TCCGGA 1 cut(s) 822
BseBI CCWGG 1 cut(s) 1529
BseCI ATCGAT 1 cut(s) 1212
BseDI CCNNGG 7 cut(s) 156, 1015, 1117, 1158, 1389, 1422, 1527
BseGI GGATG 5 cut(s) 108, 723, 736, 831, 979
BseLI CCNNNNNNNGG 7 cut(s) 299, 890, 891, 1159, 1308, 1339, 1428
BseMI GCAATG 4 cut(s) 272, 385, 1284, 1439
BseMII CTCAG 5 cut(s) 214, 287, 288, 1211, 1359
BseNI ACTGG 2 cut(s) 192, 360
BseRI GAGGAG 1 cut(s) 1580
BseXI GCAGC 3 cut(s) 661, 1084, 1190
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 1008
BshFI GGCC 2 cut(s) 1140, 1438
BshVI ATCGAT 1 cut(s) 1212
BsiEI CGRYCG 1 cut(s) 1008
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 1535
BsiSI CCGG 5 cut(s) 133, 666, 722, 823, 1389
BslFI GGGAC 2 cut(s) 907, 941
BslI CCNNNNNNNGG 7 cut(s) 299, 890, 891, 1159, 1308, 1339, 1428
BsmAI GTCTC 3 cut(s) 277, 876, 1374
BsmBI CGTCTC 2 cut(s) 277, 1374
BsmFI GGGAC 2 cut(s) 907, 941
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 2 cut(s) 1140, 1438
Bso31I GGTCTC 1 cut(s) 876
BsoBI CYCGRG 1 cut(s) 1535
Bsp1286I GDGCHC 2 cut(s) 143, 1536
Bsp13I TCCGGA 1 cut(s) 822
Bsp143I GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
Bsp1720I GCTNAGC 1 cut(s) 1220
Bsp19I CCATGG 4 cut(s) 156, 1015, 1117, 1158
Bsp68I TCGCGA 1 cut(s) 29
BspACI CCGC 6 cut(s) 136, 425, 514, 562, 924, 1427
BspANI GGCC 2 cut(s) 1140, 1438
BspCNI CTCAG 5 cut(s) 213, 287, 288, 1212, 1360
BspDI ATCGAT 1 cut(s) 1212
BspEI TCCGGA 1 cut(s) 822
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 9 cut(s) 10, 154, 192, 560, 821, 883, 1300, 1525, 1533
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 2 cut(s) 147, 160
BspQI GCTCTTC 1 cut(s) 1015
BspTI CTTAAG 1 cut(s) 1334
BspTNI GGTCTC 1 cut(s) 876
BsrDI GCAATG 4 cut(s) 272, 385, 1284, 1439
BsrI ACTGG 2 cut(s) 192, 360
BssECI CCNNGG 7 cut(s) 156, 1015, 1117, 1158, 1389, 1422, 1527
BssMI GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
BssSI CACGAG 1 cut(s) 739
BssT1I CCWWGG 4 cut(s) 156, 1015, 1117, 1158
Bst2BI CACGAG 1 cut(s) 739
Bst2UI CCWGG 1 cut(s) 1529
Bst4CI ACNGT 3 cut(s) 60, 254, 576
Bst6I CTCTTC 1 cut(s) 1015
BstAFI CTTAAG 1 cut(s) 1334
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstBAI YACGTR 1 cut(s) 1253
BstC8I GCNNGC 4 cut(s) 514, 583, 700, 1419
BstDEI CTNAG 6 cut(s) 13, 200, 274, 296, 1220, 1368
BstDSI CCRYGG 4 cut(s) 156, 1015, 1117, 1158
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 5 cut(s) 108, 723, 736, 831, 979
BstFNI CGCG 1 cut(s) 29
BstKTI GATC 7 cut(s) 155, 234, 570, 706, 756, 1004, 1542
BstMAI GTCTC 3 cut(s) 277, 876, 1374
BstMBI GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
BstMCI CGRYCG 1 cut(s) 1008
BstMWI GCNNNNNNNGC 5 cut(s) 699, 1031, 1106, 1122, 1451
BstNI CCWGG 1 cut(s) 1529
BstNSI RCATGY 2 cut(s) 913, 1417
BstSCI CCNGG 2 cut(s) 1388, 1527
BstSFI CTRYAG 2 cut(s) 546, 675
BstSNI TACGTA 1 cut(s) 1253
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 3 cut(s) 661, 1084, 1190
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
Bsu15I ATCGAT 1 cut(s) 1212
BsuI GTATCC 1 cut(s) 174
BsuRI GGCC 2 cut(s) 1140, 1438
BsuTUI ATCGAT 1 cut(s) 1212
BtgI CCRYGG 4 cut(s) 156, 1015, 1117, 1158
BtsCI GGATG 5 cut(s) 108, 723, 736, 831, 979
BtsI GCAGTG 1 cut(s) 700
BtsIMutI CAGTG 2 cut(s) 282, 700
BtuMI TCGCGA 1 cut(s) 29
Cac8I GCNNGC 4 cut(s) 514, 583, 700, 1419
CaiI CAGNNNCTG 1 cut(s) 896
Cfr13I GGNCC 3 cut(s) 191, 254, 881
ClaI ATCGAT 1 cut(s) 1212
CpoI CGGWCCG 1 cut(s) 254
CseI GACGC 2 cut(s) 259, 1506
Csp6I GTAC 2 cut(s) 241, 364
CspI CGGWCCG 1 cut(s) 254
CviQI GTAC 2 cut(s) 241, 364
DdeI CTNAG 6 cut(s) 13, 200, 274, 296, 1220, 1368
DpnI GATC 7 cut(s) 154, 233, 569, 705, 755, 1003, 1541
DpnII GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
EaeI YGGCCR 1 cut(s) 1436
Eam1104I CTCTTC 1 cut(s) 1015
EarI CTCTTC 1 cut(s) 1015
EciI GGCGGA 1 cut(s) 151
Eco105I TACGTA 1 cut(s) 1253
Eco130I CCWWGG 4 cut(s) 156, 1015, 1117, 1158
Eco24I GRGCYC 1 cut(s) 1536
Eco31I GGTCTC 1 cut(s) 876
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 3 cut(s) 191, 254, 881
Eco88I CYCGRG 1 cut(s) 1535
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 191
EcoRI GAATTC 1 cut(s) 652
EcoRII CCWGG 1 cut(s) 1527
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 4 cut(s) 156, 1015, 1117, 1158
EcoT22I ATGCAT 1 cut(s) 806
EcoT38I GRGCYC 1 cut(s) 1536
ErhI CCWWGG 4 cut(s) 156, 1015, 1117, 1158
Esp3I CGTCTC 2 cut(s) 277, 1374
FaqI GGGAC 2 cut(s) 907, 941
FauI CCCGC 3 cut(s) 521, 917, 1420
FauNDI CATATG 1 cut(s) 331
FbaI TGATCA 2 cut(s) 231, 753
Fnu4HI GCNGC 3 cut(s) 675, 1098, 1204
FokI GGATG 5 cut(s) 95, 730, 743, 838, 986
FriOI GRGCYC 1 cut(s) 1536
Fsp4HI GCNGC 3 cut(s) 675, 1098, 1204
FspBI CTAG 1 cut(s) 1326
GluI GCNGC 3 cut(s) 675, 1098, 1204
HaeIII GGCC 2 cut(s) 1140, 1438
HapII CCGG 5 cut(s) 133, 666, 722, 823, 1389
HgaI GACGC 2 cut(s) 259, 1506
HindIII AAGCTT 1 cut(s) 1187
HinfI GANTC 9 cut(s) 217, 303, 413, 467, 476, 759, 1146, 1385, 1587
HpaII CCGG 5 cut(s) 133, 666, 722, 823, 1389
HphI GGTGA 2 cut(s) 313, 1322
Hpy166II GTNNAC 3 cut(s) 21, 744, 1092
Hpy188I TCNGA 6 cut(s) 222, 236, 297, 758, 810, 881
Hpy188III TCNNGA 5 cut(s) 28, 337, 823, 958, 1039
Hpy8I GTNNAC 3 cut(s) 21, 744, 1092
HpyAV CCTTC 6 cut(s) 805, 1054, 1058, 1130, 1354, 1564
HpyCH4III ACNGT 3 cut(s) 60, 254, 576
HpyCH4IV ACGT 5 cut(s) 50, 639, 788, 1088, 1252
HpyCH4V TGCA 9 cut(s) 48, 385, 677, 698, 798, 804, 1057, 1317, 1454
HpyF10VI GCNNNNNNNGC 5 cut(s) 699, 1031, 1106, 1122, 1451
HpyF3I CTNAG 6 cut(s) 13, 200, 274, 296, 1220, 1368
HpySE526I ACGT 5 cut(s) 50, 639, 788, 1088, 1252
Kpn2I TCCGGA 1 cut(s) 822
Ksp22I TGATCA 2 cut(s) 231, 753
Kzo9I GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
LguI GCTCTTC 1 cut(s) 1015
LmnI GCTCC 2 cut(s) 138, 1531
Lsp1109I GCAGC 3 cut(s) 661, 1084, 1190
LweI GCATC 4 cut(s) 499, 708, 1021, 1044
MaeI CTAG 1 cut(s) 1326
MaeII ACGT 5 cut(s) 50, 639, 788, 1088, 1252
MaeIII GTNAC 5 cut(s) 85, 203, 851, 1328, 1363
MalI GATC 7 cut(s) 154, 233, 569, 705, 755, 1003, 1541
MboI GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
MboII GAAGA 9 cut(s) 787, 875, 1032, 1040, 1141, 1145, 1481, 1489, 1498
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 2 cut(s) 143, 1536
MlsI TGGCCA 1 cut(s) 1438
MluCI AATT 7 cut(s) 114, 125, 490, 551, 652, 1169, 1581
MluNI TGGCCA 1 cut(s) 1438
MlyI GAGTC 3 cut(s) 422, 470, 768
MmeI TCCRAC 2 cut(s) 965, 1353
Mox20I TGGCCA 1 cut(s) 1438
Mph1103I ATGCAT 1 cut(s) 806
MroI TCCGGA 1 cut(s) 822
MroXI GAANNNNTTC 1 cut(s) 1082
MscI TGGCCA 1 cut(s) 1438
MseI TTAA 2 cut(s) 456, 1335
MslI CAYNNNNRTG 1 cut(s) 1509
Msp20I TGGCCA 1 cut(s) 1438
MspA1I CMGCKG 1 cut(s) 1097
MspCI CTTAAG 1 cut(s) 1334
MspI CCGG 5 cut(s) 133, 666, 722, 823, 1389
MspR9I CCNGG 2 cut(s) 1390, 1529
Mva1269I GAATGC 1 cut(s) 804
MvaI CCWGG 1 cut(s) 1529
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 5 cut(s) 699, 1031, 1106, 1122, 1451
NciI CCSGG 1 cut(s) 1390
NcoI CCATGG 4 cut(s) 156, 1015, 1117, 1158
NdeI CATATG 1 cut(s) 331
NdeII GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
NlaIV GGNNCC 9 cut(s) 10, 154, 192, 560, 821, 883, 1300, 1525, 1533
NmeAIII GCCGAG 1 cut(s) 1403
NmuCI GTSAC 3 cut(s) 85, 851, 1328
NruI TCGCGA 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 806
NspI RCATGY 2 cut(s) 913, 1417
PciI ACATGT 1 cut(s) 909
PciSI GCTCTTC 1 cut(s) 1015
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 267
PctI GAATGC 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 6 cut(s) 217, 303, 467, 1146, 1385, 1587
PflFI GACNNNGTC 1 cut(s) 1500
PflMI CCANNNNNTGG 1 cut(s) 1159
PkrI GCNGC 3 cut(s) 676, 1099, 1205
PleI GAGTC 3 cut(s) 421, 470, 767
PpsI GAGTC 3 cut(s) 421, 470, 767
Ppu21I YACGTR 1 cut(s) 1253
PpuMI RGGWCCY 1 cut(s) 191
PscI ACATGT 1 cut(s) 909
Psp5II RGGWCCY 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 1527
PspGI CCWGG 1 cut(s) 1527
PspN4I GGNNCC 9 cut(s) 10, 154, 192, 560, 821, 883, 1300, 1525, 1533
PspPI GGNCC 3 cut(s) 191, 254, 881
PspPPI RGGWCCY 1 cut(s) 191
PstI CTGCAG 1 cut(s) 679
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 1 cut(s) 152
PsyI GACNNNGTC 1 cut(s) 1500
PvuII CAGCTG 1 cut(s) 1097
RruI TCGCGA 1 cut(s) 29
RsaI GTAC 2 cut(s) 242, 365
RsaNI GTAC 2 cut(s) 241, 364
RseI CAYNNNNRTG 1 cut(s) 1509
Rsr2I CGGWCCG 1 cut(s) 254
RsrII CGGWCCG 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 1015
SaqAI TTAA 2 cut(s) 456, 1335
SatI GCNGC 3 cut(s) 675, 1098, 1204
Sau3AI GATC 7 cut(s) 152, 231, 567, 703, 753, 1001, 1539
Sau96I GGNCC 3 cut(s) 191, 254, 881
SchI GAGTC 3 cut(s) 422, 470, 768
ScrFI CCNGG 2 cut(s) 1390, 1529
SduI GDGCHC 2 cut(s) 143, 1536
SfaNI GCATC 4 cut(s) 499, 708, 1021, 1044
SfcI CTRYAG 2 cut(s) 546, 675
SinI GGWCC 3 cut(s) 191, 254, 881
SmiMI CAYNNNNRTG 1 cut(s) 1509
SmlI CTYRAG 2 cut(s) 1190, 1334
SmoI CTYRAG 2 cut(s) 1190, 1334
SnaBI TACGTA 1 cut(s) 1253
SpeI ACTAGT 1 cut(s) 1325
Sse9I AATT 7 cut(s) 114, 125, 490, 551, 652, 1169, 1581
SsiI CCGC 6 cut(s) 136, 425, 514, 562, 924, 1427
SspMI CTAG 1 cut(s) 1326
StyD4I CCNGG 2 cut(s) 1388, 1527
StyI CCWWGG 4 cut(s) 156, 1015, 1117, 1158
TaaI ACNGT 3 cut(s) 60, 254, 576
TaiI ACGT 5 cut(s) 53, 642, 791, 1091, 1255
TaqI TCGA 6 cut(s) 261, 411, 525, 906, 1212, 1542
TaqII GACCGA 1 cut(s) 994
TasI AATT 7 cut(s) 114, 125, 490, 551, 652, 1169, 1581
TfiI GAWTC 6 cut(s) 217, 303, 467, 1146, 1385, 1587
Tru1I TTAA 2 cut(s) 456, 1335
Tru9I TTAA 2 cut(s) 456, 1335
TscAI CASTG 2 cut(s) 282, 700
TseFI GTSAC 3 cut(s) 85, 851, 1328
TseI GCWGC 3 cut(s) 674, 1097, 1203
Tsp45I GTSAC 3 cut(s) 85, 851, 1328
TspDTI ATGAA 4 cut(s) 300, 587, 636, 788
TspGWI ACGGA 2 cut(s) 246, 381
TspRI CASTG 2 cut(s) 282, 700
Tth111I GACNNNGTC 1 cut(s) 1500
Van91I CCANNNNNTGG 1 cut(s) 1159
Vha464I CTTAAG 1 cut(s) 1334
VpaK11BI GGWCC 3 cut(s) 191, 254, 881
XagI CCTNNNNNAGG 1 cut(s) 297
XapI RAATTY 4 cut(s) 114, 125, 652, 1169
XceI RCATGY 2 cut(s) 913, 1417
XcmI CCANNNNNNNNNTGG 1 cut(s) 1556
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 1 cut(s) 1326
Zsp2I ATGCAT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.