Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0015941

Physical Location & Seq

Genomic Coordinates Forward (+)

2186275 .. 2188791

Gene Structure 2517 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g03320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2517 bp

ATGGCTTCTCTCTTCCCAAACACCACAATTCCCAACCCAAATATCTCTCTCCCCAAACACTCTCTTCCCACAGAACCCCACAAAATCTCACAAACCCAATTCACAAAACCCCCAGAAAATGCGAAAAGCAATCAAATTCTGTACCAATCGTACTTTAGTCGCATGTCATCTCTTTGCAAGCAAGGCCAAATTCAACAGGCTGTGGACTTGGTCGCCGAAATGGAGCTCAAAAACCTCCGCATCGGGCCTGAAGTCTACGGCGAGCTCGTCCAGGGGTGCGTCTACGAGCGAGCTCTTCATACGGGTAGGCAAATTCACGCCCAGATTGTCAAGAAAGGTGCCATCTTTGCGATGAATGAGTACATTGAAACCAAGTTAGTGATTTTCTACGCGAAATGCGATAATCCGGAGGCTTCAAATAGTTTGTTTAGGAGGGTGAGGTTGAAAAATGTGTTTTCTTGGGCTGCTGTTATTGGGTTGAATTGCAGGAAGGGTTTTCATGAAGAGGCTTTGCTAGGGTTTAGGGAAATGCAGGAAAATGGGTTGCTGCCGGACAATTTTGTGCTTCCGAATGTGTTGAAGGCTTGTGGTGGTTTGGAGTGGATTCGGATTGGGAAAGTGGTTCATGGGTTGGTGGTGAAGTTAGGTTGTGGTGGGTGTGTGTTTGTTGCTAGTAGTCTTGTGGATATGTATGGAAAATGCGGGAAGGTGGAGGATGCAAGAAAGGTGTTTGATGGTATGCCCGAAAGAAATGCAGTCGCTTGGAATTCGATGATTGTGGGTTATGTACAAAACGGTTTGAATGAGGAAGCTATTGAGGTGTTTTATGAGATGAGGGAGGAGGGGGTTGAGCCCACTCAAGTTACTATATCGAGCTTTCTTTCTGCTTCGGCTAATTTAGGCGCATTGCAAGAAGGTAAGCAAGGTCATGCAATTGCCGTGATATGTGGAATAGAAATGACGACCAATTTGGGGAGTTCTCTTATAAACTTTTATTCCAAGGTTGGTTTGATTGAGGATGCGGAATCGGTTTTTAGTAGGATGCTTGAGAAAGATGTGGTCACATGGAATTTGCTCATATCTGGATATGTGCAAATTGGGGAAGTTGACAAAGCACTGAATATGTGCCGTTTAATGAGACTCGAAAACTTGAGGTTTGACTCTGTGTGTCTTGCAACCTTAATGTCTGCTTTTGCAGATACGCGTAATTTGAAGTTGGGTAAGGAGGGACATTGCTATTGTGTAAGGAACAACCTTGAATCCGACGTGGTTGTTGTGAGTAGCATTGTAGACATGTATGCCAAATGTGAGAAAATCGGTTGTGCAAGACGAGTTTTCAACTCTTCGATAACGAAAGATCTTATTCTGTGGAACACAATGCTGGCTGCTTTTGCAGAATTGGGTCACAGTGGTGAGGCCTTAAATTTATTCTATCAGATGCAGCTAGAAAGCATGCCGCCAAATGTGATATCATGGAACTCTCTGATTTTAGGGTTTCTGAATGGTGGCCAGGTCAATGAGGCTAAAGATATGTTTTTGCAAATGCAGTCCCTTGTAGTCCAACCTAACCTGGTCACTTGGACTACCCTGATCTCGGGCCTGGCTAGGAGTGGTTTTGGCTACGAAGCGATTCTAACATTCCAGCGGATGCAAGAAGCTGGAGTTAAACCGAATGTTGTGAGCATTATTGGTGTACTCTTGGCTTGCATAAACTTGGCATCATTACAGATTGGAAGAGCCTTACATGGATATTTGATACGGCATTCACTCTACTTGTCAATTCCAATCGCGACCTCTTTGGTAAATATGTATGCTAAATGTGGCAATAGAGATCAAGCGAAGAGGGTGTTTGATATGATTCCTGACAAGGAGTTACCAATCTACAATGCAATGATTTCCGGTTTTGCATTACACGGTCAAGCTGTGGAAGCTCTTGCACTATATCGGTGCTTAAAAGAAGAAGGTTTGAAACCCGACAACATAACCTTTACCAATGCGCTATACGCATGTAGCCATGCCATGATGGTTAGTGAAGGGTTGGAGCTTTTTGTTGATATGGTGTCCAATCACAATATAAATCCGAGTATAGAGCATTATGGCTGTATGGTTAGTCTTCTTTCGCGGTGTGGAGATCTGGTTGAAGCTTTTAGGCTCATTAGCACGATGCCTTACAAGCCTGATGTACAGATATTGGGATCATTACTTGCTGCTTGTAGGGAACACAATAAGATCGAACTCGAAGAATATCTATCCAATCAACTACTGAAATTACAGCCAGACAATTCCGGAAACTATGTAGCAATGTCAAATGCATATGCAGCTGCAGGAAGATGGGATGAGGTTAAAAAGGTGAGGCAGTTAATGAAAGAAAGGGGCTTGCGAAAAATTCCCGGATGCAGCTGGATTCAAGTCGGAGAAGAACTTAATGTGTTTGTTGCTGGTGACAAGTCACACCCTGAAACCGAAGAAATCTATACGACATTGGCATTGTTGCGAATGGAAATGTCTTTTCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

839

Amino Acids

93.23

Weight (kDa)

6.32

Isoelectric Point (pI)

37.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	251 - 297	1.9e-11	PPR repeat family
PPR	PF01535	253 - 283	2.7e-09	PPR repeat
PPR_2	PF13041	351 - 399	1.1e-07	PPR repeat family
PPR	PF01535	354 - 383	1e-05	PPR repeat
PPR_2	PF13041	487 - 536	1.6e-12	PPR repeat family
PPR_3	PF13812	514 - 561	8.6e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	522 - 562	5.4e-07	PPR repeat family
PPR	PF01535	525 - 555	2.9e-06	PPR repeat
PPR_2	PF13041	627 - 671	3.5e-07	PPR repeat family
E_motif	PF20431	741 - 803	1.5e-22	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55740
fragaria_vesca	FvH4_1g04140
malus_domestica	MD02G1039900.v1.1
prunus_persica	Prupe.7G235800_v2.0.a1
pyrus_communis	pycom02g03320
rosa_chinensis	RchiOBHm_Chr2g0089611
rosa_laevigata	RLG00000016045
rosa_multiflora	Rmu_sc0004662.1_g000003
rosa_roxburghii	Rroxscaffold_2G00151710
rosa_rugosa	Rorug02G0002100
rosa_samantha	Rh2AG047400 Rh2BG046100 Rh2CG048000 Rh2DG047500
rosa_wichuraiana	Rw2G003950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	986
AccB1I	GGYRCC	1 cut(s)	338
AccI	GTMKAC	3 cut(s)	255, 282, 1290
AccII	CGCG	4 cut(s)	392, 1204, 1790, 2122
AccIII	TCCGGA	2 cut(s)	406, 2285
AciI	CCGC	6 cut(s)	238, 702, 1022, 1457, 1645, 2122
AclWI	GGATC	1 cut(s)	2203
AcoI	YGGCCR	1 cut(s)	1507
AcsI	RAATTY	7 cut(s)	135, 189, 312, 766, 1069, 1423, 2385
AcuI	CTGAAG	1 cut(s)	270
AfaI	GTAC	6 cut(s)	143, 152, 362, 789, 1695, 2184
AfiI	CCNNNNNNNGG	2 cut(s)	972, 1594
AflIII	ACRYGT	2 cut(s)	1202, 1293
AjiI	CACGTC	1 cut(s)	1267
AjnI	CCWGG	4 cut(s)	270, 1509, 1569, 1599
AleI	CACNNNNGTG	1 cut(s)	1410
Alw21I	GWGCWC	3 cut(s)	228, 267, 295
Alw26I	GTCTC	1 cut(s)	1132
AlwI	GGATC	1 cut(s)	2203
Ama87I	CYCGRG	1 cut(s)	1594
Aor13HI	TCCGGA	2 cut(s)	406, 2285
AoxI	GGCC	5 cut(s)	184, 245, 1416, 1507, 1597
ApeKI	GCWGC	8 cut(s)	464, 547, 1385, 1441, 2207, 2318, 2321, 2397
ApoI	RAATTY	7 cut(s)	135, 189, 312, 766, 1069, 1423, 2385
ArsI	GACNNNNNNTTYG	2 cut(s)	952, 984
Asp700I	GAANNNNTTC	1 cut(s)	1629
AspLEI	GCGC	2 cut(s)	905, 1999
AspS9I	GGNCC	2 cut(s)	245, 1597
AsuC2I	CCSGG	1 cut(s)	2391
AsuHPI	GGTGA	5 cut(s)	448, 649, 1424, 2362, 2453
AvaI	CYCGRG	1 cut(s)	1594
BalI	TGGCCA	1 cut(s)	1509
BanI	GGYRCC	1 cut(s)	338
BanII	GRGCYC	4 cut(s)	228, 267, 295, 855
BbsI	GAAGAC	1 cut(s)	2105
Bbv12I	GWGCWC	3 cut(s)	228, 267, 295
BbvI	GCAGC	8 cut(s)	451, 534, 1372, 1453, 2194, 2308, 2330, 2409
BccI	CCATC	4 cut(s)	350, 728, 2017, 2325
BceAI	ACGGC	4 cut(s)	274, 923, 1113, 1775
BcgI	CGANNNNNNTGC	2 cut(s)	734, 768
BciT130I	CCWGG	4 cut(s)	272, 1511, 1571, 1601
BcnI	CCSGG	1 cut(s)	2391
BcoDI	GTCTC	1 cut(s)	1132
BfaI	CTAG	4 cut(s)	515, 672, 1445, 1605
BfmI	CTRYAG	1 cut(s)	2322
BglII	AGATCT	2 cut(s)	1357, 2131
BisI	GCNGC	9 cut(s)	465, 548, 1386, 1442, 1457, 2208, 2319, 2322, 2398
BlsI	GCNGC	9 cut(s)	466, 549, 1387, 1443, 1458, 2209, 2320, 2323, 2399
Bme1390I	CCNGG	5 cut(s)	272, 1511, 1571, 1601, 2391
BmeT110I	CYCGRG	1 cut(s)	1594
BmgBI	CACGTC	1 cut(s)	1267
BmgT120I	GGNCC	2 cut(s)	245, 1597
BmiI	GGNNCC	1 cut(s)	340
BmrFI	CCNGG	5 cut(s)	272, 1511, 1571, 1601, 2391
BmsI	GCATC	9 cut(s)	249, 706, 1009, 1032, 1428, 1638, 1727, 2154, 2384
BpiI	GAAGAC	1 cut(s)	2105
BpmI	CTGGAG	1 cut(s)	1680
BpuEI	CTTGAG	3 cut(s)	843, 1067, 1171
BpuMI	CCSGG	1 cut(s)	2391
BsaJI	CCNNGG	2 cut(s)	271, 999
BsaWI	WCCGGW	3 cut(s)	406, 1898, 2285
BsaXI	ACNNNNNCTCC	2 cut(s)	2121, 2151
Bsc4I	CCNNNNNNNGG	2 cut(s)	972, 1594
Bse3DI	GCAATG	4 cut(s)	905, 1231, 1896, 2307
BseAI	TCCGGA	2 cut(s)	406, 2285
BseBI	CCWGG	4 cut(s)	272, 1511, 1571, 1601
BseDI	CCNNGG	2 cut(s)	271, 999
BseGI	GGATG	6 cut(s)	721, 1024, 1047, 1653, 2341, 2399
BseLI	CCNNNNNNNGG	2 cut(s)	972, 1594
BseMI	GCAATG	4 cut(s)	905, 1231, 1896, 2307
BseRI	GAGGAG	1 cut(s)	854
BseXI	GCAGC	8 cut(s)	451, 534, 1372, 1453, 2194, 2308, 2330, 2409
Bsh1236I	CGCG	4 cut(s)	392, 1204, 1790, 2122
BshFI	GGCC	5 cut(s)	186, 247, 1418, 1509, 1599
BshNI	GGYRCC	1 cut(s)	338
BsiHKAI	GWGCWC	3 cut(s)	228, 267, 295
BsiHKCI	CYCGRG	1 cut(s)	1594
BsiSI	CCGG	5 cut(s)	407, 551, 1899, 2286, 2391
BslFI	GGGAC	2 cut(s)	1242, 1534
BslI	CCNNNNNNNGG	2 cut(s)	972, 1594
BsmAI	GTCTC	1 cut(s)	1132
BsmFI	GGGAC	2 cut(s)	1242, 1534
BsmI	GAATGC	1 cut(s)	1762
BsnI	GGCC	5 cut(s)	186, 247, 1418, 1509, 1599
BsoBI	CYCGRG	1 cut(s)	1594
Bsp1286I	GDGCHC	4 cut(s)	228, 267, 295, 855
Bsp13I	TCCGGA	2 cut(s)	406, 2285
Bsp1407I	TGTACA	2 cut(s)	787, 2182
Bsp143I	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
Bsp68I	TCGCGA	1 cut(s)	1790
BspACI	CCGC	6 cut(s)	238, 702, 1022, 1457, 1645, 2122
BspANI	GGCC	5 cut(s)	186, 247, 1418, 1509, 1599
BspEI	TCCGGA	2 cut(s)	406, 2285
BspFNI	CGCG	4 cut(s)	392, 1204, 1790, 2122
BspHI	TCATGA	1 cut(s)	499
BspLI	GGNNCC	1 cut(s)	340
BspMAI	CTGCAG	1 cut(s)	2326
BspPI	GGATC	1 cut(s)	2203
BspQI	GCTCTTC	2 cut(s)	300, 1729
BspT107I	GGYRCC	1 cut(s)	338
BsrDI	GCAATG	4 cut(s)	905, 1231, 1896, 2307
BsrGI	TGTACA	2 cut(s)	787, 2182
BssECI	CCNNGG	2 cut(s)	271, 999
BssMI	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
BssT1I	CCWWGG	1 cut(s)	999
Bst2UI	CCWGG	4 cut(s)	272, 1511, 1571, 1601
Bst4CI	ACNGT	3 cut(s)	797, 1409, 1916
Bst6I	CTCTTC	7 cut(s)	17, 69, 300, 498, 1348, 1729, 1835
BstAUI	TGTACA	2 cut(s)	787, 2182
BstC8I	GCNNGC	7 cut(s)	179, 263, 291, 1383, 1454, 1705, 2378
BstF5I	GGATG	6 cut(s)	721, 1024, 1047, 1653, 2341, 2399
BstFNI	CGCG	4 cut(s)	392, 1204, 1790, 2122
BstHHI	GCGC	2 cut(s)	905, 1999
BstKTI	GATC	6 cut(s)	1360, 1593, 1834, 2134, 2198, 2232
BstMAI	GTCTC	1 cut(s)	1132
BstMBI	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
BstMWI	GCNNNNNNNGC	7 cut(s)	183, 347, 1391, 1928, 2003, 2173, 2318
BstNI	CCWGG	4 cut(s)	272, 1511, 1571, 1601
BstNSI	RCATGY	4 cut(s)	166, 1297, 1456, 2010
BstSCI	CCNGG	5 cut(s)	270, 1509, 1569, 1599, 2389
BstSFI	CTRYAG	1 cut(s)	2322
BstUI	CGCG	4 cut(s)	392, 1204, 1790, 2122
BstV1I	GCAGC	8 cut(s)	451, 534, 1372, 1453, 2194, 2308, 2330, 2409
BstV2I	GAAGAC	1 cut(s)	2105
BstX2I	RGATCY	2 cut(s)	1357, 2131
BstYI	RGATCY	2 cut(s)	1357, 2131
BsuRI	GGCC	5 cut(s)	186, 247, 1418, 1509, 1599
BtgZI	GCGATG	1 cut(s)	365
BtrI	CACGTC	1 cut(s)	1267
BtsCI	GGATG	6 cut(s)	721, 1024, 1047, 1653, 2341, 2399
BtsIMutI	CAGTG	2 cut(s)	1115, 1414
BtuMI	TCGCGA	1 cut(s)	1790
Cac8I	GCNNGC	7 cut(s)	179, 263, 291, 1383, 1454, 1705, 2378
CciI	TCATGA	1 cut(s)	499
CfoI	GCGC	2 cut(s)	905, 1999
Cfr13I	GGNCC	2 cut(s)	245, 1597
CseI	GACGC	1 cut(s)	268
CsiI	ACCWGGT	1 cut(s)	1569
Csp6I	GTAC	6 cut(s)	142, 151, 361, 788, 1694, 2183
CviQI	GTAC	6 cut(s)	142, 151, 361, 788, 1694, 2183
DpnI	GATC	6 cut(s)	1359, 1592, 1833, 2133, 2197, 2231
DpnII	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
EaeI	YGGCCR	1 cut(s)	1507
Eam1104I	CTCTTC	7 cut(s)	17, 69, 300, 498, 1348, 1729, 1835
EarI	CTCTTC	7 cut(s)	17, 69, 300, 498, 1348, 1729, 1835
Ecl136II	GAGCTC	3 cut(s)	226, 265, 293
Eco130I	CCWWGG	1 cut(s)	999
Eco147I	AGGCCT	1 cut(s)	1418
Eco24I	GRGCYC	4 cut(s)	228, 267, 295, 855
Eco32I	GATATC	1 cut(s)	1470
Eco53kI	GAGCTC	3 cut(s)	226, 265, 293
Eco57I	CTGAAG	1 cut(s)	270
Eco88I	CYCGRG	1 cut(s)	1594
EcoICRI	GAGCTC	3 cut(s)	226, 265, 293
EcoRI	GAATTC	1 cut(s)	766
EcoRII	CCWGG	4 cut(s)	270, 1509, 1569, 1599
EcoRV	GATATC	1 cut(s)	1470
EcoT14I	CCWWGG	1 cut(s)	999
EcoT22I	ATGCAT	1 cut(s)	2314
EcoT38I	GRGCYC	4 cut(s)	228, 267, 295, 855
ErhI	CCWWGG	1 cut(s)	999
FaqI	GGGAC	2 cut(s)	1242, 1534
FauI	CCCGC	1 cut(s)	695
FauNDI	CATATG	1 cut(s)	2314
FblI	GTMKAC	3 cut(s)	255, 282, 1290
Fnu4HI	GCNGC	9 cut(s)	465, 548, 1386, 1442, 1457, 2208, 2319, 2322, 2398
FokI	GGATG	6 cut(s)	728, 1031, 1054, 1660, 2348, 2406
FriOI	GRGCYC	4 cut(s)	228, 267, 295, 855
Fsp4HI	GCNGC	9 cut(s)	465, 548, 1386, 1442, 1457, 2208, 2319, 2322, 2398
FspBI	CTAG	4 cut(s)	515, 672, 1445, 1605
GlaI	GCGC	2 cut(s)	904, 1998
GluI	GCNGC	9 cut(s)	465, 548, 1386, 1442, 1457, 2208, 2319, 2322, 2398
GsuI	CTGGAG	1 cut(s)	1680
HaeIII	GGCC	5 cut(s)	186, 247, 1418, 1509, 1599
HapII	CCGG	5 cut(s)	407, 551, 1899, 2286, 2391
HgaI	GACGC	1 cut(s)	268
HhaI	GCGC	2 cut(s)	905, 1999
Hin6I	GCGC	2 cut(s)	903, 1997
HinP1I	GCGC	2 cut(s)	903, 1997
HincII	GTYRAC	1 cut(s)	1108
HindII	GTYRAC	1 cut(s)	1108
HindIII	AAGCTT	1 cut(s)	2142
HinfI	GANTC	8 cut(s)	604, 1025, 1140, 1160, 1259, 1630, 1858, 2404
HpaII	CCGG	5 cut(s)	407, 551, 1899, 2286, 2391
HphI	GGTGA	5 cut(s)	448, 649, 1424, 2362, 2453
Hpy166II	GTNNAC	6 cut(s)	205, 256, 283, 1108, 1291, 1694
Hpy188I	TCNGA	8 cut(s)	570, 609, 1264, 1437, 1485, 1500, 2082, 2414
Hpy188III	TCNNGA	7 cut(s)	331, 407, 500, 1083, 1789, 1862, 2286
Hpy8I	GTNNAC	6 cut(s)	205, 256, 283, 1108, 1291, 1694
Hpy99I	CGWCG	1 cut(s)	1268
HpyAV	CCTTC	6 cut(s)	484, 574, 700, 908, 1955, 2027
HpyCH4III	ACNGT	3 cut(s)	797, 1409, 1916
HpyCH4IV	ACGT	1 cut(s)	1266
HpyF10VI	GCNNNNNNNGC	7 cut(s)	183, 347, 1391, 1928, 2003, 2173, 2318
HpySE526I	ACGT	1 cut(s)	1266
HspAI	GCGC	2 cut(s)	903, 1997
Kpn2I	TCCGGA	2 cut(s)	406, 2285
Kzo9I	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
LguI	GCTCTTC	2 cut(s)	300, 1729
LmnI	GCTCC	2 cut(s)	223, 2041
Lsp1109I	GCAGC	8 cut(s)	451, 534, 1372, 1453, 2194, 2308, 2330, 2409
LweI	GCATC	9 cut(s)	249, 706, 1009, 1032, 1428, 1638, 1727, 2154, 2384
MabI	ACCWGGT	1 cut(s)	1569
MaeI	CTAG	4 cut(s)	515, 672, 1445, 1605
MaeII	ACGT	1 cut(s)	1266
MaeIII	GTNAC	7 cut(s)	862, 1060, 1403, 1573, 1872, 2441, 2448
MalI	GATC	6 cut(s)	1359, 1592, 1833, 2133, 2197, 2231
MboI	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
MfeI	CAATTG	1 cut(s)	933
MflI	RGATCY	2 cut(s)	1357, 2131
MhlI	GDGCHC	4 cut(s)	228, 267, 295, 855
MlsI	TGGCCA	1 cut(s)	1509
MluI	ACGCGT	1 cut(s)	1202
MluNI	TGGCCA	1 cut(s)	1509
MlyI	GAGTC	2 cut(s)	1134, 1154
MmeI	TCCRAC	4 cut(s)	1287, 1585, 2019, 2392
Mox20I	TGGCCA	1 cut(s)	1509
Mph1103I	ATGCAT	1 cut(s)	2314
MroI	TCCGGA	2 cut(s)	406, 2285
MroXI	GAANNNNTTC	1 cut(s)	1629
MscI	TGGCCA	1 cut(s)	1509
MseI	TTAA	8 cut(s)	1133, 1181, 1421, 1665, 1952, 2343, 2360, 2424
MslI	CAYNNNNRTG	1 cut(s)	1410
Msp20I	TGGCCA	1 cut(s)	1509
MspA1I	CMGCKG	3 cut(s)	1645, 2321, 2400
MspI	CCGG	5 cut(s)	407, 551, 1899, 2286, 2391
MspR9I	CCNGG	5 cut(s)	272, 1511, 1571, 1601, 2391
MunI	CAATTG	1 cut(s)	933
Mva1269I	GAATGC	1 cut(s)	1762
MvaI	CCWGG	4 cut(s)	272, 1511, 1571, 1601
MvnI	CGCG	4 cut(s)	392, 1204, 1790, 2122
MwoI	GCNNNNNNNGC	7 cut(s)	183, 347, 1391, 1928, 2003, 2173, 2318
NciI	CCSGG	1 cut(s)	2391
NdeI	CATATG	1 cut(s)	2314
NdeII	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
NlaIV	GGNNCC	1 cut(s)	340
NmuCI	GTSAC	5 cut(s)	1060, 1403, 1573, 2441, 2448
NruI	TCGCGA	1 cut(s)	1790
NsiI	ATGCAT	1 cut(s)	2314
NspI	RCATGY	4 cut(s)	166, 1297, 1456, 2010
OliI	CACNNNNGTG	1 cut(s)	1410
PaeI	GCATGC	1 cut(s)	1456
PagI	TCATGA	1 cut(s)	499
PceI	AGGCCT	1 cut(s)	1418
PciI	ACATGT	1 cut(s)	1293
PciSI	GCTCTTC	2 cut(s)	300, 1729
PcsI	WCGNNNNNNNCGW	2 cut(s)	264, 396
PctI	GAATGC	1 cut(s)	1762
PdmI	GAANNNNTTC	1 cut(s)	1629
PfeI	GAWTC	6 cut(s)	604, 1025, 1259, 1630, 1858, 2404
PflFI	GACNNNGTC	1 cut(s)	209
PfoI	TCCNGGA	1 cut(s)	2389
PkrI	GCNGC	9 cut(s)	466, 549, 1387, 1443, 1458, 2209, 2320, 2323, 2399
PleI	GAGTC	2 cut(s)	1134, 1154
PpsI	GAGTC	2 cut(s)	1134, 1154
PscI	ACATGT	1 cut(s)	1293
PsiI	TTATAA	1 cut(s)	986
Psp124BI	GAGCTC	3 cut(s)	228, 267, 295
Psp6I	CCWGG	4 cut(s)	270, 1509, 1569, 1599
PspGI	CCWGG	4 cut(s)	270, 1509, 1569, 1599
PspN4I	GGNNCC	1 cut(s)	340
PspPI	GGNCC	2 cut(s)	245, 1597
PstI	CTGCAG	1 cut(s)	2326
PsuI	RGATCY	2 cut(s)	1357, 2131
PsyI	GACNNNGTC	1 cut(s)	209
PvuII	CAGCTG	2 cut(s)	2321, 2400
RruI	TCGCGA	1 cut(s)	1790
RsaI	GTAC	6 cut(s)	143, 152, 362, 789, 1695, 2184
RsaNI	GTAC	6 cut(s)	142, 151, 361, 788, 1694, 2183
RseI	CAYNNNNRTG	1 cut(s)	1410
SacI	GAGCTC	3 cut(s)	228, 267, 295
SapI	GCTCTTC	2 cut(s)	300, 1729
SaqAI	TTAA	8 cut(s)	1133, 1181, 1421, 1665, 1952, 2343, 2360, 2424
SatI	GCNGC	9 cut(s)	465, 548, 1386, 1442, 1457, 2208, 2319, 2322, 2398
Sau3AI	GATC	6 cut(s)	1357, 1590, 1831, 2131, 2195, 2229
Sau96I	GGNCC	2 cut(s)	245, 1597
SchI	GAGTC	2 cut(s)	1134, 1154
ScrFI	CCNGG	5 cut(s)	272, 1511, 1571, 1601, 2391
SduI	GDGCHC	4 cut(s)	228, 267, 295, 855
SexAI	ACCWGGT	1 cut(s)	1569
SfaNI	GCATC	9 cut(s)	249, 706, 1009, 1032, 1428, 1638, 1727, 2154, 2384
SfcI	CTRYAG	1 cut(s)	2322
SmiMI	CAYNNNNRTG	1 cut(s)	1410
SmlI	CTYRAG	3 cut(s)	858, 1046, 1150
SmoI	CTYRAG	3 cut(s)	858, 1046, 1150
SphI	GCATGC	1 cut(s)	1456
SseBI	AGGCCT	1 cut(s)	1418
SsiI	CCGC	6 cut(s)	238, 702, 1022, 1457, 1645, 2122
SspMI	CTAG	4 cut(s)	515, 672, 1445, 1605
SstI	GAGCTC	3 cut(s)	228, 267, 295
StuI	AGGCCT	1 cut(s)	1418
StyD4I	CCNGG	5 cut(s)	270, 1509, 1569, 1599, 2389
StyI	CCWWGG	1 cut(s)	999
TaaI	ACNGT	3 cut(s)	797, 1409, 1916
TaiI	ACGT	1 cut(s)	1269
TaqI	TCGA	6 cut(s)	770, 872, 1143, 1346, 2232, 2238
TatI	WGTACW	4 cut(s)	360, 787, 1693, 2182
TauI	GCSGC	1 cut(s)	1459
TfiI	GAWTC	6 cut(s)	604, 1025, 1259, 1630, 1858, 2404
Tru1I	TTAA	8 cut(s)	1133, 1181, 1421, 1665, 1952, 2343, 2360, 2424
Tru9I	TTAA	8 cut(s)	1133, 1181, 1421, 1665, 1952, 2343, 2360, 2424
TscAI	CASTG	2 cut(s)	1122, 1414
TseFI	GTSAC	5 cut(s)	1060, 1403, 1573, 2441, 2448
TseI	GCWGC	8 cut(s)	464, 547, 1385, 1441, 2207, 2318, 2321, 2397
Tsp45I	GTSAC	5 cut(s)	1060, 1403, 1573, 2441, 2448
TspDTI	ATGAA	6 cut(s)	287, 368, 488, 516, 614, 2378
TspRI	CASTG	2 cut(s)	1122, 1414
Tth111I	GACNNNGTC	1 cut(s)	209
XapI	RAATTY	7 cut(s)	135, 189, 312, 766, 1069, 1423, 2385
XceI	RCATGY	4 cut(s)	166, 1297, 1456, 2010
XmiI	GTMKAC	3 cut(s)	255, 282, 1290
XmnI	GAANNNNTTC	1 cut(s)	1629
XspI	CTAG	4 cut(s)	515, 672, 1445, 1605
Zsp2I	ATGCAT	1 cut(s)	2314

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.