Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0015941

Physical Location & Seq

Genomic Coordinates Forward (+)

3274173 .. 3278165

Gene Structure 3993 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG046100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2580 bp

ATGCCCTTCCCGCTTTTATCTGGGATTAACGAACGACCAACCAACCATTATCTTGGTCTAGCTTTGCTCTGTTTCTCTTCACTCATGGCTTCTTTACCTTACAGCACCATTCCAAACACACACCTCTCTTCATCCAAACACTCCATACCCATAGAAAACCAAAAACCATCACAAGCCCATTTCCCAAAGCTTCAAGAAAATGCAACCCAGAATCAAACTCTGTCCAAATCTTACTTCAATCAGATGTCAACTCTCTCCAGACAGAGCCAAATTCAAGAAGCCGTAGACTTACTCATCCATATGGACCTCAAGAGCCTCCACATTGGGCCCGAAATCTACGGCGAGCTGCTTCAGGCGTGTGTCTACGAGCGAGCTCTTCAAACCGGAAAGCAAATCCACGCCCGGATTATCAAGAAAGGTGAGCTGTTTTCAACAAATGAGTACATTGAAACTAAACTAGTGATCTTTTACGCAAAATGCGATGATGGGAAAGCTTCGAATCGTTTGTTTAGAAGGGTGAGATTGAAAAATGTGTTTTCTTGGGCTGCAGTTGTTGGATTGAATTGTAGATTGGGTTTTTACAAAGAGGCTTTGCTAGGGTTTATGGAAATGCATGAAGATGGGTTGCTTCCGGATAATTTTGTGGTTCCGAATGTGTTGAAGGCTTGCGGGGCGATGGAGTGGATTGGAGTTGGGAAAGCAGTTCATGGATATGTGGTCAAGATGGGTTGTAATGGGTGTGTGTTTGTTGCTAGTAGTCTTGTAGATATGTATGGGAAATGTGGGGTTGTGGATGATGCGAGGAAGGTGTTTGATGAAATGGATGGGAGAAATGTGATCACATGGAATTCGATGCTTGTGAGTTATGTGCAAAATGGATTGAATGAGGATGCTATCAAGGTGTTTTGTGATATGAGGGAGGAAGGTGTTGAACCTACCCATGTGACTGTGTCTAGCTTTCTGTCGGCTTCGGCTAATTTAGGTGCGCTAGAAGAAGGTAAGCAAGGGCATGCATTAGCAGTTGTGAGTGGACTTGAACTGAATACCATTTTGGGGAGTTCTATTATAAACTTTTATTCCAAGGTTGGTTTGATTGAGGATGCTGAAATAGTTTTTAGTAGGATGAATGAGAAAGATGTGGTTACATGGAATTTGCTCATATCTGGGTATGTGCAAATTGGGGAGGTTGACAAAGCTGTCGAGATGTGCCGCTTAATGAGACTAGAAAACTTGAGGTTTGACTCGGTGACTCTTGCATCCCTAATGTCTGCTTTTGCAGATACACGAAATTTGAAATTTGGCAAGGAGGCGCACTGTTATTGTATAAGAAACAACCTTGAGGATGATGTGGTTGTTGCAAGTAGCATTGTAGACTTGTATTCCAAATGTGAGAAAATTGATTATGCAAGACAAGCTTTCGAATCTGTAACAACTGGAGATCTTGTACTGTGGAACACACTGTTGGCGGCTTATGCAGGATTGGGACATAGTGGTGAGGCATTGAAATTGTTCTATCAGATGCAACTGGAGAGTGTGCCTCCAAATGTTATGACTTGGAACTCTCTGATATTTGGTTTTTTGAAAAATGACCAGGTCAATGAGGCTCAAGAAATGTTCCTGCAGATGCAGTCCCTTGGTGTCGAGCCAAACCTGGTAACTTGGACTACTATGATCTCTGGTCTGGCAGAGAATGGTTTTGGCCATGAAGCAATTCAGGCCTTCCATCGGATGCAAGAAGCGGGAATCAAACCCAATGTTGTGAGTATCATTTGTGTACTTAAGGCTTGTATAGAAATGGCATCATTACAGAATGGAAGAGTTATACATGGGTATTTGATTAGGCATTCCCTCTACCTTTCAATCCCAATTGCAACGTCTTTAGTGGATATGTATGCTAAATGTGGTAATGTAGAAGAAGCAAAGAGAGTGTTTGATATGGTGTCAGACAAGGAGTTACCAATCTATAATGCAATGATCTCCAGTTACGCGTTACATGGTCAAGCTGTGGAAGCTCTTGCCCTATACCAGCGTTTAAAGGAAGAAGGCTTACAACCCGACAGCGTAACCTTTACTAATGCCTTATATGCATGTAGCCATGCCAGTCTAGTTAGTGAAGGTTTGGAGCTTTTGGATGACTTGTTATCCAATCAAACTATAAATCCAAGTATTGAGCACTATGGTTGTGTGGTTAATCTTCTTTCTCGCTGTGGCAATGTGGATGAAGCTTTTAGGCTTATTGTCGTGATGCCTTATGAGCCTGATGCACAGATATTAGGATCATTACTTGCAGCTTGTAGAGAACAGAATAACATAGAACTAGAGGAGTATCTGTCCGATCAATTACTGAAATTAGAGCCAGAGAATTCTGGAAACTATGTAGCGATTTCAAATGCATATGCAGTTGCAGGAAGATGGGATGAGGTAAAGAAAGTGAGGCAGTTAATGAAAGAAAAGGGCCTGAGAAAGATTCCTGGATGCAGCTGGATTCAAATTGGAGAAGAAATCCATGCGTTTGTTGCTGGTGACAAATCACACCCAGAAACTGAGCAAATCTATATGACATTGGAATTGTTGGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016071 GO:0016556 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

859

Amino Acids

95.62

Weight (kDa)

5.28

Isoelectric Point (pI)

35.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	277 - 324	1.1e-11	PPR repeat family
PPR_3	PF13812	278 - 323	2.9e-06	Pentatricopeptide repeat domain
PPR	PF01535	280 - 310	2.1e-07	PPR repeat
PPR_2	PF13041	378 - 426	6.3e-09	PPR repeat family
PPR_1	PF12854	379 - 407	8.7e-06	PPR repeat
PPR	PF01535	381 - 410	3.9e-06	PPR repeat
PPR_2	PF13041	480 - 523	1.9e-08	PPR repeat family
PPR_long	PF17177	499 - 645	8.8e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	512 - 542	7.2e-06	PPR repeat
PPR_2	PF13041	514 - 562	4.2e-15	PPR repeat family
PPR	PF01535	518 - 547	5.1e-06	PPR repeat
PPR_3	PF13812	541 - 596	7.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	546 - 577	1.3e-06	PPR repeat
PPR_2	PF13041	549 - 596	3.7e-10	PPR repeat family
PPR	PF01535	552 - 582	3e-07	PPR repeat
PPR_2	PF13041	654 - 698	5e-08	PPR repeat family
E_motif	PF20431	769 - 830	4.8e-23	E motif
Eplus_motif	PF20430	835 - 856	1.1e-06	E+ motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55740
fragaria_vesca	FvH4_1g04140
malus_domestica	MD02G1039900.v1.1
prunus_persica	Prupe.7G235800_v2.0.a1
pyrus_communis	pycom02g03320
rosa_chinensis	RchiOBHm_Chr2g0089611
rosa_laevigata	RLG00000016045
rosa_multiflora	Rmu_sc0004662.1_g000003
rosa_roxburghii	Rroxscaffold_2G00151710
rosa_rugosa	Rorug02G0002100
rosa_samantha	Rh2AG047400 Rh2BG046100 Rh2CG048000 Rh2DG047500
rosa_wichuraiana	Rw2G003950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1067
AasI	GACNNNNNNGTC	1 cut(s)	1196
AccI	GTMKAC	3 cut(s)	285, 363, 1371
AccII	CGCG	1 cut(s)	1988
AccIII	TCCGGA	1 cut(s)	631
AciI	CCGC	5 cut(s)	11, 669, 1210, 1466, 1739
AclWI	GGATC	1 cut(s)	2284
AcoI	YGGCCR	1 cut(s)	1699
AcsI	RAATTY	6 cut(s)	270, 847, 1150, 1288, 1295, 2362
AcuI	CTGAAG	1 cut(s)	335
AfaI	GTAC	3 cut(s)	443, 1446, 1776
AfiI	CCNNNNNNNGG	2 cut(s)	1053, 1725
AflII	CTTAAG	1 cut(s)	1778
AflIII	ACRYGT	1 cut(s)	1986
AhlI	ACTAGT	1 cut(s)	457
AjnI	CCWGG	3 cut(s)	1590, 1650, 2470
AjuI	GAANNNNNNNTTGG	8 cut(s)	218, 250, 554, 586, 1034, 1066, 1445, 1477
Alw21I	GWGCWC	2 cut(s)	376, 2175
Alw26I	GTCTC	1 cut(s)	1213
AlwI	GGATC	1 cut(s)	2284
AlwNI	CAGNNNCTG	1 cut(s)	2543
Aor13HI	TCCGGA	1 cut(s)	631
AoxI	GGCC	4 cut(s)	326, 1699, 1716, 2455
ApaI	GGGCCC	1 cut(s)	330
ApeKI	GCWGC	4 cut(s)	346, 545, 2288, 2478
ApoI	RAATTY	6 cut(s)	270, 847, 1150, 1288, 1295, 2362
Asp700I	GAANNNNTTC	2 cut(s)	1508, 1710
AspLEI	GCGC	2 cut(s)	988, 1312
AspS9I	GGNCC	4 cut(s)	304, 326, 327, 2455
AsuC2I	CCSGG	1 cut(s)	403
AsuHPI	GGTGA	5 cut(s)	431, 529, 1258, 1505, 2534
AsuII	TTCGAA	2 cut(s)	497, 1419
AvaII	GGWCC	1 cut(s)	304
BaeGI	GKGCMC	1 cut(s)	330
BalI	TGGCCA	1 cut(s)	1701
BanII	GRGCYC	2 cut(s)	330, 376
BarI	GAAGNNNNNNTAC	2 cut(s)	2031, 2063
Bbv12I	GWGCWC	2 cut(s)	376, 2175
BbvI	GCAGC	4 cut(s)	333, 532, 2300, 2490
BccI	CCATC	8 cut(s)	175, 479, 614, 670, 718, 818, 1731, 2406
BceAI	ACGGC	2 cut(s)	266, 355
BciT130I	CCWGG	3 cut(s)	1592, 1652, 2472
BclI	TGATCA	1 cut(s)	837
BcnI	CCSGG	1 cut(s)	403
BcoDI	GTCTC	1 cut(s)	1213
BcuI	ACTAGT	1 cut(s)	457
BfaI	CTAG	9 cut(s)	59, 458, 596, 753, 954, 989, 1223, 2105, 2318
BfmI	CTRYAG	2 cut(s)	546, 1619
BfrI	CTTAAG	1 cut(s)	1778
BglII	AGATCT	1 cut(s)	1438
BisI	GCNGC	6 cut(s)	347, 546, 1210, 1467, 2289, 2479
BlsI	GCNGC	6 cut(s)	348, 547, 1211, 1468, 2290, 2480
Bme1390I	CCNGG	4 cut(s)	403, 1592, 1652, 2472
Bme18I	GGWCC	1 cut(s)	304
BmgT120I	GGNCC	4 cut(s)	304, 326, 327, 2455
BmiI	GGNNCC	2 cut(s)	328, 648
BmrFI	CCNGG	4 cut(s)	403, 1592, 1652, 2472
BplI	GAGNNNNNCTC	2 cut(s)	1522, 1554
BpmI	CTGGAG	4 cut(s)	241, 1455, 1547, 1963
Bpu14I	TTCGAA	2 cut(s)	497, 1419
BpuEI	CTTGAG	4 cut(s)	293, 1252, 1358, 1590
BpuMI	CCSGG	1 cut(s)	403
BsaJI	CCNNGG	2 cut(s)	1080, 1633
BsaWI	WCCGGW	2 cut(s)	383, 631
BsaXI	ACNNNNNCTCC	4 cut(s)	1428, 1458, 2315, 2345
Bsc4I	CCNNNNNNNGG	2 cut(s)	1053, 1725
Bse1I	ACTGG	4 cut(s)	1438, 1530, 1980, 2100
Bse3DI	GCAATG	2 cut(s)	1977, 2218
BseAI	TCCGGA	1 cut(s)	631
BseBI	CCWGG	3 cut(s)	1592, 1652, 2472
BseDI	CCNNGG	2 cut(s)	1080, 1633
BseLI	CCNNNNNNNGG	2 cut(s)	1053, 1725
BseMI	GCAATG	2 cut(s)	1977, 2218
BseMII	CTCAG	2 cut(s)	2450, 2535
BseNI	ACTGG	4 cut(s)	1438, 1530, 1980, 2100
BseRI	GAGGAG	1 cut(s)	2336
BseSI	GKGCMC	1 cut(s)	330
BseXI	GCAGC	4 cut(s)	333, 532, 2300, 2490
Bsh1236I	CGCG	1 cut(s)	1988
BshFI	GGCC	4 cut(s)	328, 1701, 1718, 2457
BsiHKAI	GWGCWC	2 cut(s)	376, 2175
BsiSI	CCGG	3 cut(s)	384, 403, 632
BslFI	GGGAC	2 cut(s)	1497, 1615
BslI	CCNNNNNNNGG	2 cut(s)	1053, 1725
BsmAI	GTCTC	1 cut(s)	1213
BsmFI	GGGAC	2 cut(s)	1497, 1615
BsmI	GAATGC	1 cut(s)	1843
BsnI	GGCC	4 cut(s)	328, 1701, 1718, 2457
Bsp119I	TTCGAA	2 cut(s)	497, 1419
Bsp120I	GGGCCC	1 cut(s)	326
Bsp1286I	GDGCHC	3 cut(s)	330, 376, 2175
Bsp13I	TCCGGA	1 cut(s)	631
Bsp143I	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
BspACI	CCGC	5 cut(s)	11, 669, 1210, 1466, 1739
BspANI	GGCC	4 cut(s)	328, 1701, 1718, 2457
BspCNI	CTCAG	2 cut(s)	2451, 2536
BspEI	TCCGGA	1 cut(s)	631
BspFNI	CGCG	1 cut(s)	1988
BspLI	GGNNCC	2 cut(s)	328, 648
BspMAI	CTGCAG	2 cut(s)	550, 1623
BspPI	GGATC	1 cut(s)	2284
BspQI	GCTCTTC	1 cut(s)	381
BspT104I	TTCGAA	2 cut(s)	497, 1419
BspTI	CTTAAG	1 cut(s)	1778
BsrDI	GCAATG	2 cut(s)	1977, 2218
BsrI	ACTGG	4 cut(s)	1438, 1530, 1980, 2100
BssECI	CCNNGG	2 cut(s)	1080, 1633
BssMI	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
BssT1I	CCWWGG	2 cut(s)	1080, 1633
Bst2UI	CCWGG	3 cut(s)	1592, 1652, 2472
Bst4CI	ACNGT	4 cut(s)	949, 1316, 1449, 1461
Bst6I	CTCTTC	4 cut(s)	82, 133, 381, 1810
BstAFI	CTTAAG	1 cut(s)	1778
BstBI	TTCGAA	2 cut(s)	497, 1419
BstC8I	GCNNGC	4 cut(s)	344, 372, 667, 1011
BstDEI	CTNAG	2 cut(s)	2459, 2544
BstFNI	CGCG	1 cut(s)	1988
BstHHI	GCGC	2 cut(s)	988, 1312
BstKTI	GATC	7 cut(s)	465, 840, 1441, 1674, 1977, 2279, 2338
BstMAI	GTCTC	1 cut(s)	1213
BstMBI	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
BstMWI	GCNNNNNNNGC	9 cut(s)	10, 671, 1412, 1472, 1715, 2009, 2084, 2254, 2516
BstNI	CCWGG	3 cut(s)	1592, 1652, 2472
BstNSI	RCATGY	2 cut(s)	1013, 2091
BstSCI	CCNGG	4 cut(s)	401, 1590, 1650, 2470
BstSFI	CTRYAG	2 cut(s)	546, 1619
BstSLI	GKGCMC	1 cut(s)	330
BstUI	CGCG	1 cut(s)	1988
BstV1I	GCAGC	4 cut(s)	333, 532, 2300, 2490
BstX2I	RGATCY	1 cut(s)	1438
BstXI	CCANNNNNNTGG	1 cut(s)	53
BstYI	RGATCY	1 cut(s)	1438
BsuRI	GGCC	4 cut(s)	328, 1701, 1718, 2457
BtgZI	GCGATG	2 cut(s)	495, 689
BtsIMutI	CAGTG	2 cut(s)	1312, 1457
Cac8I	GCNNGC	4 cut(s)	344, 372, 667, 1011
CaiI	CAGNNNCTG	1 cut(s)	2543
CfoI	GCGC	2 cut(s)	988, 1312
Cfr13I	GGNCC	4 cut(s)	304, 326, 327, 2455
CsiI	ACCWGGT	2 cut(s)	1590, 1650
Csp6I	GTAC	3 cut(s)	442, 1445, 1775
CviQI	GTAC	3 cut(s)	442, 1445, 1775
DdeI	CTNAG	2 cut(s)	2459, 2544
DpnI	GATC	7 cut(s)	464, 839, 1440, 1673, 1976, 2278, 2337
DpnII	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
DraI	TTTAAA	1 cut(s)	2034
DrdI	GACNNNNNNGTC	1 cut(s)	1196
DseDI	GACNNNNNNGTC	1 cut(s)	1196
EaeI	YGGCCR	1 cut(s)	1699
Eam1104I	CTCTTC	4 cut(s)	82, 133, 381, 1810
EarI	CTCTTC	4 cut(s)	82, 133, 381, 1810
Ecl136II	GAGCTC	1 cut(s)	374
Eco130I	CCWWGG	2 cut(s)	1080, 1633
Eco147I	AGGCCT	1 cut(s)	1718
Eco24I	GRGCYC	2 cut(s)	330, 376
Eco47I	GGWCC	1 cut(s)	304
Eco53kI	GAGCTC	1 cut(s)	374
Eco57I	CTGAAG	1 cut(s)	335
EcoICRI	GAGCTC	1 cut(s)	374
EcoO109I	RGGNCCY	1 cut(s)	2455
EcoRI	GAATTC	2 cut(s)	847, 2362
EcoRII	CCWGG	3 cut(s)	1590, 1650, 2470
EcoT14I	CCWWGG	2 cut(s)	1080, 1633
EcoT22I	ATGCAT	4 cut(s)	615, 1015, 2089, 2395
EcoT38I	GRGCYC	2 cut(s)	330, 376
ErhI	CCWWGG	2 cut(s)	1080, 1633
FalI	AAGNNNNNCTT	2 cut(s)	1399, 1431
FaqI	GGGAC	2 cut(s)	1497, 1615
FauI	CCCGC	3 cut(s)	18, 662, 1732
FauNDI	CATATG	2 cut(s)	300, 2395
FbaI	TGATCA	1 cut(s)	837
FblI	GTMKAC	3 cut(s)	285, 363, 1371
Fnu4HI	GCNGC	6 cut(s)	347, 546, 1210, 1467, 2289, 2479
FriOI	GRGCYC	2 cut(s)	330, 376
Fsp4HI	GCNGC	6 cut(s)	347, 546, 1210, 1467, 2289, 2479
FspBI	CTAG	9 cut(s)	59, 458, 596, 753, 954, 989, 1223, 2105, 2318
GlaI	GCGC	2 cut(s)	987, 1311
GluI	GCNGC	6 cut(s)	347, 546, 1210, 1467, 2289, 2479
GsuI	CTGGAG	4 cut(s)	241, 1455, 1547, 1963
HaeIII	GGCC	4 cut(s)	328, 1701, 1718, 2457
HapII	CCGG	3 cut(s)	384, 403, 632
HhaI	GCGC	2 cut(s)	988, 1312
Hin6I	GCGC	2 cut(s)	986, 1310
HinP1I	GCGC	2 cut(s)	986, 1310
HincII	GTYRAC	2 cut(s)	249, 1189
HindII	GTYRAC	2 cut(s)	249, 1189
HindIII	AAGCTT	4 cut(s)	188, 492, 1413, 2223
HinfI	GANTC	8 cut(s)	211, 499, 1241, 1249, 1421, 1743, 2467, 2485
HpaII	CCGG	3 cut(s)	384, 403, 632
HphI	GGTGA	5 cut(s)	431, 529, 1258, 1505, 2534
Hpy166II	GTNNAC	7 cut(s)	249, 286, 364, 1031, 1189, 1372, 1775
Hpy188I	TCNGA	7 cut(s)	243, 651, 1518, 1566, 1728, 1945, 2335
Hpy8I	GTNNAC	7 cut(s)	249, 286, 364, 1031, 1189, 1372, 1775
HpyAV	CCTTC	9 cut(s)	16, 507, 655, 799, 917, 989, 1729, 2036, 2108
HpyCH4III	ACNGT	4 cut(s)	949, 1316, 1449, 1461
HpyCH4IV	ACGT	1 cut(s)	1874
HpyF10VI	GCNNNNNNNGC	9 cut(s)	10, 671, 1412, 1472, 1715, 2009, 2084, 2254, 2516
HpyF3I	CTNAG	2 cut(s)	2459, 2544
HpySE526I	ACGT	1 cut(s)	1874
HspAI	GCGC	2 cut(s)	986, 1310
Kpn2I	TCCGGA	1 cut(s)	631
Ksp22I	TGATCA	1 cut(s)	837
Kzo9I	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
LguI	GCTCTTC	1 cut(s)	381
LmnI	GCTCC	1 cut(s)	2122
Lsp1109I	GCAGC	4 cut(s)	333, 532, 2300, 2490
MabI	ACCWGGT	2 cut(s)	1590, 1650
MaeI	CTAG	9 cut(s)	59, 458, 596, 753, 954, 989, 1223, 2105, 2318
MaeII	ACGT	1 cut(s)	1874
MalI	GATC	7 cut(s)	464, 839, 1440, 1673, 1976, 2278, 2337
MboI	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
MfeI	CAATTG	1 cut(s)	1866
MflI	RGATCY	1 cut(s)	1438
MhlI	GDGCHC	3 cut(s)	330, 376, 2175
MlsI	TGGCCA	1 cut(s)	1701
MluI	ACGCGT	1 cut(s)	1986
MluNI	TGGCCA	1 cut(s)	1701
MlyI	GAGTC	2 cut(s)	1235, 1243
MmeI	TCCRAC	2 cut(s)	535, 2553
Mox20I	TGGCCA	1 cut(s)	1701
Mph1103I	ATGCAT	4 cut(s)	615, 1015, 2089, 2395
MroI	TCCGGA	1 cut(s)	631
MroXI	GAANNNNTTC	2 cut(s)	1508, 1710
MscI	TGGCCA	1 cut(s)	1701
MseI	TTAA	6 cut(s)	27, 1214, 1779, 2033, 2190, 2441
MslI	CAYNNNNRTG	4 cut(s)	299, 618, 711, 1491
Msp20I	TGGCCA	1 cut(s)	1701
MspA1I	CMGCKG	1 cut(s)	2481
MspCI	CTTAAG	1 cut(s)	1778
MspI	CCGG	3 cut(s)	384, 403, 632
MspR9I	CCNGG	4 cut(s)	403, 1592, 1652, 2472
MunI	CAATTG	1 cut(s)	1866
Mva1269I	GAATGC	1 cut(s)	1843
MvaI	CCWGG	3 cut(s)	1592, 1652, 2472
MvnI	CGCG	1 cut(s)	1988
MwoI	GCNNNNNNNGC	9 cut(s)	10, 671, 1412, 1472, 1715, 2009, 2084, 2254, 2516
NciI	CCSGG	1 cut(s)	403
NdeI	CATATG	2 cut(s)	300, 2395
NdeII	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
NlaIV	GGNNCC	2 cut(s)	328, 648
NmuCI	GTSAC	3 cut(s)	943, 1246, 2522
NsiI	ATGCAT	4 cut(s)	615, 1015, 2089, 2395
NspI	RCATGY	2 cut(s)	1013, 2091
NspV	TTCGAA	2 cut(s)	497, 1419
PaeI	GCATGC	1 cut(s)	1013
PceI	AGGCCT	1 cut(s)	1718
PciSI	GCTCTTC	1 cut(s)	381
PcsI	WCGNNNNNNNCGW	1 cut(s)	477
PctI	GAATGC	1 cut(s)	1843
PdmI	GAANNNNTTC	2 cut(s)	1508, 1710
PfeI	GAWTC	6 cut(s)	211, 499, 1421, 1743, 2467, 2485
PflFI	GACNNNGTC	2 cut(s)	949, 1592
PfoI	TCCNGGA	1 cut(s)	2470
PkrI	GCNGC	6 cut(s)	348, 547, 1211, 1468, 2290, 2480
PleI	GAGTC	2 cut(s)	1235, 1243
PpsI	GAGTC	2 cut(s)	1235, 1243
PsiI	TTATAA	1 cut(s)	1067
Psp124BI	GAGCTC	1 cut(s)	376
Psp6I	CCWGG	3 cut(s)	1590, 1650, 2470
PspGI	CCWGG	3 cut(s)	1590, 1650, 2470
PspN4I	GGNNCC	2 cut(s)	328, 648
PspOMI	GGGCCC	1 cut(s)	326
PspPI	GGNCC	4 cut(s)	304, 326, 327, 2455
PstI	CTGCAG	2 cut(s)	550, 1623
PstNI	CAGNNNCTG	1 cut(s)	2543
PsuI	RGATCY	1 cut(s)	1438
PsyI	GACNNNGTC	2 cut(s)	949, 1592
PvuII	CAGCTG	1 cut(s)	2481
RsaI	GTAC	3 cut(s)	443, 1446, 1776
RsaNI	GTAC	3 cut(s)	442, 1445, 1775
RseI	CAYNNNNRTG	4 cut(s)	299, 618, 711, 1491
SacI	GAGCTC	1 cut(s)	376
SapI	GCTCTTC	1 cut(s)	381
SaqAI	TTAA	6 cut(s)	27, 1214, 1779, 2033, 2190, 2441
SatI	GCNGC	6 cut(s)	347, 546, 1210, 1467, 2289, 2479
Sau3AI	GATC	7 cut(s)	462, 837, 1438, 1671, 1974, 2276, 2335
Sau96I	GGNCC	4 cut(s)	304, 326, 327, 2455
SchI	GAGTC	2 cut(s)	1235, 1243
ScrFI	CCNGG	4 cut(s)	403, 1592, 1652, 2472
SduI	GDGCHC	3 cut(s)	330, 376, 2175
SexAI	ACCWGGT	2 cut(s)	1590, 1650
SfcI	CTRYAG	2 cut(s)	546, 1619
SfuI	TTCGAA	2 cut(s)	497, 1419
SinI	GGWCC	1 cut(s)	304
SmiMI	CAYNNNNRTG	4 cut(s)	299, 618, 711, 1491
SmlI	CTYRAG	5 cut(s)	308, 1231, 1337, 1605, 1778
SmoI	CTYRAG	5 cut(s)	308, 1231, 1337, 1605, 1778
SpeI	ACTAGT	1 cut(s)	457
SphI	GCATGC	1 cut(s)	1013
SseBI	AGGCCT	1 cut(s)	1718
SsiI	CCGC	5 cut(s)	11, 669, 1210, 1466, 1739
SspMI	CTAG	9 cut(s)	59, 458, 596, 753, 954, 989, 1223, 2105, 2318
SstI	GAGCTC	1 cut(s)	376
StuI	AGGCCT	1 cut(s)	1718
StyD4I	CCNGG	4 cut(s)	401, 1590, 1650, 2470
StyI	CCWWGG	2 cut(s)	1080, 1633
TaaI	ACNGT	4 cut(s)	949, 1316, 1449, 1461
TaiI	ACGT	1 cut(s)	1877
TaqI	TCGA	5 cut(s)	497, 851, 1200, 1419, 1641
TatI	WGTACW	3 cut(s)	441, 1444, 1774
TauI	GCSGC	2 cut(s)	1212, 1469
TfiI	GAWTC	6 cut(s)	211, 499, 1421, 1743, 2467, 2485
Tru1I	TTAA	6 cut(s)	27, 1214, 1779, 2033, 2190, 2441
Tru9I	TTAA	6 cut(s)	27, 1214, 1779, 2033, 2190, 2441
TscAI	CASTG	2 cut(s)	1319, 1464
TseFI	GTSAC	3 cut(s)	943, 1246, 2522
TseI	GCWGC	4 cut(s)	346, 545, 2288, 2478
Tsp45I	GTSAC	3 cut(s)	943, 1246, 2522
TspDTI	ATGAA	8 cut(s)	120, 630, 695, 831, 1139, 1719, 2235, 2459
TspRI	CASTG	2 cut(s)	1319, 1464
Tth111I	GACNNNGTC	2 cut(s)	949, 1592
Vha464I	CTTAAG	1 cut(s)	1778
VpaK11BI	GGWCC	1 cut(s)	304
XapI	RAATTY	6 cut(s)	270, 847, 1150, 1288, 1295, 2362
XceI	RCATGY	2 cut(s)	1013, 2091
XmiI	GTMKAC	3 cut(s)	285, 363, 1371
XmnI	GAANNNNTTC	2 cut(s)	1508, 1710
XspI	CTAG	9 cut(s)	59, 458, 596, 753, 954, 989, 1223, 2105, 2318
Zsp2I	ATGCAT	4 cut(s)	615, 1015, 2089, 2395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.